LOCUS       NM_001128               6602 bp    mRNA    linear   PRI 29-APR-2025
DEFINITION  Homo sapiens adaptor related protein complex 1 subunit gamma 1
            (AP1G1), transcript variant 2, mRNA.
ACCESSION   NM_001128
VERSION     NM_001128.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   Hoshi,N., Uemura,T., Tachibana,K., Abe,S., Murakami-Nishimagi,Y.,
            Okano,M., Noda,M., Saito,K., Kono,K., Ohtake,T. and Waguri,S.
  TITLE     Endosomal protein expression of gamma1-adaptin is associated with
            tumor growth activity and relapse-free survival in breast cancer
  JOURNAL   Breast Cancer 31 (2), 305-316 (2024)
   PUBMED   38265632
  REMARK    GeneRIF: Endosomal protein expression of gamma1-adaptin is
            associated with tumor growth activity and relapse-free survival in
            breast cancer.
REFERENCE   2  (bases 1 to 6602)
  AUTHORS   Usmani,M.A., Ahmed,Z.M., Magini,P., Pienkowski,V.M.,
            Rasmussen,K.J., Hernan,R., Rasheed,F., Hussain,M., Shahzad,M.,
            Lanpher,B.C., Niu,Z., Lim,F.Y., Pippucci,T., Ploski,R., Kraus,V.,
            Matuszewska,K., Palombo,F., Kianmahd,J., Martinez-Agosto,J.A.,
            Lee,H., Colao,E., Motazacker,M.M., Brigatti,K.W.,
            Puffenberger,E.G., Riazuddin,S.A., Gonzaga-Jauregui,C., Chung,W.K.,
            Wagner,M., Schultz,M.J., Seri,M., Kievit,A.J.A., Perrotti,N.,
            Wassink-Ruiter,J.S.K., van Bokhoven,H., Riazuddin,S. and
            Riazuddin,S.
  CONSRTM   UCLA Clinical Genomics Center
  TITLE     De novo and bi-allelic variants in AP1G1 cause neurodevelopmental
            disorder with developmental delay, intellectual disability, and
            epilepsy
  JOURNAL   Am J Hum Genet 108 (7), 1330-1341 (2021)
   PUBMED   34102099
  REMARK    GeneRIF: De novo and bi-allelic variants in AP1G1 cause
            neurodevelopmental disorder with developmental delay, intellectual
            disability, and epilepsy.
REFERENCE   3  (bases 1 to 6602)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 6602)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 6602)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 6602)
  AUTHORS   Hirst,J., Lui,W.W., Bright,N.A., Totty,N., Seaman,M.N. and
            Robinson,M.S.
  TITLE     A family of proteins with gamma-adaptin and VHS domains that
            facilitate trafficking between the trans-Golgi network and the
            vacuole/lysosome
  JOURNAL   J Cell Biol 149 (1), 67-80 (2000)
   PUBMED   10747088
REFERENCE   7  (bases 1 to 6602)
  AUTHORS   Folsch,H., Ohno,H., Bonifacino,J.S. and Mellman,I.
  TITLE     A novel clathrin adaptor complex mediates basolateral targeting in
            polarized epithelial cells
  JOURNAL   Cell 99 (2), 189-198 (1999)
   PUBMED   10535737
REFERENCE   8  (bases 1 to 6602)
  AUTHORS   Page,L.J., Sowerby,P.J., Lui,W.W. and Robinson,M.S.
  TITLE     Gamma-synergin: an EH domain-containing protein that interacts with
            gamma-adaptin
  JOURNAL   J Cell Biol 146 (5), 993-1004 (1999)
   PUBMED   10477754
REFERENCE   9  (bases 1 to 6602)
  AUTHORS   Takatsu,H., Sakurai,M., Shin,H.W., Murakami,K. and Nakayama,K.
  TITLE     Identification and characterization of novel clathrin
            adaptor-related proteins
  JOURNAL   J Biol Chem 273 (38), 24693-24700 (1998)
   PUBMED   9733768
REFERENCE   10 (bases 1 to 6602)
  AUTHORS   Peyrard,M., Parveneh,S., Lagercrantz,S., Ekman,M., Fransson,I.,
            Sahlen,S. and Dumanski,J.P.
  TITLE     Cloning, expression pattern, and chromosomal assignment to 16q23 of
            the human gamma-adaptin gene (ADTG)
  JOURNAL   Genomics 50 (2), 275-280 (1998)
   PUBMED   9653655
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BU902252.1, AB015317.1,
            BC036283.1 and AC009097.9.
            
            On Jun 2, 2019 this sequence version replaced NM_001128.5.
            
            Summary: Adaptins are important components of clathrin-coated
            vesicles transporting ligand-receptor complexes from the plasma
            membrane or from the trans-Golgi network to lysosomes. The adaptin
            family of proteins is composed of four classes of molecules named
            alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together
            with medium and small subunits, form a heterotetrameric complex
            called an adaptor, whose role is to promote the formation of
            clathrin-coated pits and vesicles. The protein encoded by this gene
            is a gamma-adaptin protein and it belongs to the adaptor complexes
            large subunits family. Two transcript variants encoding different
            isoforms have been found for this gene. [provided by RefSeq, Jul
            2008].
            
            Transcript Variant: This variant (2) lacks an in-frame exon
            compared to variant 1. The resulting isoform (b) has the same N-
            and C-termini but is shorter compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853559.12715.1,
                                           SRR11853562.13898.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000299980.9/ ENSP00000299980.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-640               BU902252.1         240-879
            641-1727            AB015317.1         585-1671
            1728-4088           BC036283.1         1941-4301
            4089-6602           AC009097.9         189781-192294       c
FEATURES             Location/Qualifiers
     source          1..6602
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q22.2"
     gene            1..6602
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /note="adaptor related protein complex 1 subunit gamma 1"
                     /db_xref="GeneID:164"
                     /db_xref="HGNC:HGNC:555"
                     /db_xref="MIM:603533"
     exon            1..72
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     exon            73..276
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     CDS             76..2544
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /note="isoform b is encoded by transcript variant 2;
                     clathrin-associated/assembly/adaptor protein, large, gamma
                     1; gamma1-adaptin; golgi adaptor HA1/AP1 adaptin gamma
                     subunit; clathrin assembly protein complex 1 gamma large
                     chain; gamma adaptin; AP-1 complex subunit gamma-1;
                     adaptor protein complex AP-1 subunit gamma-1; golgi
                     adaptor HA1/AP1 adaptin subunit gamma-1; testicular tissue
                     protein Li 21; adaptor related protein complex 1 gamma 1
                     subunit; adapter-related protein complex 1 subunit
                     gamma-1; clathrin assembly protein complex 1 gamma-1 large
                     chain"
                     /codon_start=1
                     /product="AP-1 complex subunit gamma-1 isoform b"
                     /protein_id="NP_001119.3"
                     /db_xref="CCDS:CCDS32480.1"
                     /db_xref="GeneID:164"
                     /db_xref="HGNC:HGNC:555"
                     /db_xref="MIM:603533"
                     /translation="MPAPIRLRELIRTIRTARTQAEEREMIQKECAAIRSSFREEDNT
                     YRCRNVAKLLYMHMLGYPAHFGQLECLKLIASQKFTDKRIGYLGAMLLLDERQDVHLL
                     MTNCIKNDLNHSTQFVQGLALCTLGCMGSSEMCRDLAGEVEKLLKTSNSYLRKKAALC
                     AVHVIRKVPELMEMFLPATKNLLNEKNHGVLHTSVVLLTEMCERSPDMLAHFRKLVPQ
                     LVRILKNLIMSGYSPEHDVSGISDPFLQVRILRLLRILGRNDDDSSEAMNDILAQVAT
                     NTETSKNVGNAILYETVLTIMDIKSESGLRVLAINILGRFLLNNDKNIRYVALTSLLK
                     TVQTDHNAVQRHRSTIVDCLKDLDVSIKRRAMELSFALVNGNNIRGMMKELLYFLDSC
                     EPEFKADCASGIFLAAEKYAPSKRWHIDTIMRVLTTAGSYVRDDAVPNLIQLITNSVE
                     MHAYTVQRLYKAILGDYSQQPLVQVAAWCIGEYGDLLVSGQCEEEEPIQVTEDEVLDI
                     LESVLISNMSTSVTRGYALTAIMKLSTRFTCTVNRIKKVVSIYGSSIDVELQQRAVEY
                     NALFKKYDHMRSALLERMPVMEKVTTNGPTEIVQTNGETEPAPLETKPPPSGPQPTSQ
                     ANDLLDLLGGNDITPVIPTAPTSKPSSAGGELLDLLGDINLTGAPAAAPAPASVPQIS
                     QPPFLLDGLSSQPLFNDIAAGIPSITAYSKNGLKIEFTFERSNTNPSVTVITIQASNS
                     TELDMTDFVFQAAVPKTFQLQLLSPSSSIVPAFNTGTITQVIKVLNPQKQQLRMRIKL
                     TYNHKGSAMQDLAEVNNFPPQSWQ"
     exon            277..401
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     exon            402..543
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     exon            544..640
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     exon            641..717
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     exon            718..813
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     exon            814..894
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     exon            895..993
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1049
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     exon            1050..1163
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1304
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     exon            1305..1359
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..1482
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     exon            1483..1572
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     exon            1573..1700
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     exon            1701..1805
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     exon            1806..1947
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     exon            1948..2074
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     exon            2075..2182
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     exon            2183..2343
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     exon            2344..2442
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     exon            2443..6602
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3698..3703
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /note="hexamer: AATAAA"
     polyA_site      3722
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /note="major polyA site"
     regulatory      6573..6578
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /note="hexamer: AATAAA"
     polyA_site      6602
                     /gene="AP1G1"
                     /gene_synonym="ADTG; CLAPG1; USRISD"
                     /note="major polyA site"
ORIGIN      
        1 ggatgttcgg ttcctgctgc cactgctgcc gccgcccccg gagctgctgg tttcattcga
       61 ggtttcgggc cgaggatgcc agcccccatc agattgcggg agctgatccg gaccatccgg
      121 acagcccgaa cccaagctga agaacgagaa atgatccaga aagaatgtgc tgcaatccgg
      181 tcatctttta gagaagaaga caatacatac cgatgtcgga atgtggcaaa attactgtat
      241 atgcacatgc tgggctaccc tgctcacttt ggacagttgg agtgcctcaa gcttattgcc
      301 tctcaaaaat ttacagacaa acgcattggc tatttagggg caatgctgct gttagatgaa
      361 agacaagatg tccatcttct catgaccaac tgtatcaaga atgatcttaa tcatagcacg
      421 caattcgtac aggggttagc actttgtacc ctcggctgca tgggctcctc agagatgtgc
      481 agagatcttg caggagaggt agagaagctc ctgaaaacct ccaactctta cttaagaaaa
      541 aaggcagcac tgtgtgctgt tcatgtcatc aggaaagttc ctgaacttat ggagatgttt
      601 ttaccagcaa caaaaaattt attgaatgag aagaaccatg gtgtcctcca cacatctgta
      661 gtcctcctca cagaaatgtg tgagcgaagc ccagacatgc ttgcgcattt cagaaagctt
      721 gtgccccaat tagttcgtat tttaaagaac ctcatcatgt ccggatattc accagaacat
      781 gatgtttctg gtatcagtga cccctttttg caggtacgaa ttttgcggtt attaagaatt
      841 ttaggacgaa atgatgatga ttcaagtgaa gctatgaatg atatattagc acaggttgcc
      901 actaatactg agactagtaa aaatgtagga aatgctattc tttatgaaac ggttttgact
      961 atcatggata ttaagtcaga gagtggattg cgagtcctag ccataaatat cctgggtcgt
     1021 ttcttattga acaatgacaa gaatattaga tatgtggctc tgacatcttt gttgaagact
     1081 gtacagacag atcataatgc agtacagagg cacagaagca caattgtgga ctgtcttaaa
     1141 gatttggatg tctcaataaa acggcgtgca atggaattga gttttgccct ggtaaatggg
     1201 aataatatcc gaggcatgat gaaagaatta ctttattttc tggattcgtg tgagccagaa
     1261 tttaaagcag actgtgcatc tggaatcttt cttgctgcag aaaagtatgc accttccaaa
     1321 cgatggcata tagacacaat tatgcgtgtt ttgacaacgg caggaagtta tgttcgtgat
     1381 gatgcagtcc ccaatttaat ccagttaata actaatagtg tggagatgca tgcctatact
     1441 gtccagcgcc tgtacaaagc aattcttggt gattattctc aacaaccttt ggtacaagtg
     1501 gctgcatggt gtataggtga atatggtgat cttcttgtat ctggccagtg tgaagaggaa
     1561 gagcctattc aggtaacaga ggatgaagtg ttggatattt tagaaagtgt cctaatctct
     1621 aatatgtcca cctctgtgac acgaggttat gccctcactg ccattatgaa gctttccact
     1681 cgattcactt gtactgtaaa ccgaattaag aaagtggttt ccatctacgg aagcagcatt
     1741 gatgtggaac tccagcagag ggcagtagaa tataatgcac ttttcaagaa atatgaccac
     1801 atgaggtctg ccctacttga gagaatgcct gtcatggaaa aagtgaccac aaatggccct
     1861 actgagattg tgcagacaaa tggagagaca gaaccagctc cactagagac caaaccgcca
     1921 ccctctgggc cacagcccac cagccaggcc aatgatttat tggatttgtt gggaggaaat
     1981 gacataacac ctgttattcc aactgcgcct acaagcaaac catcttctgc tggtggagaa
     2041 cttcttgatt tgctgggaga catcaacctt acaggtgctc cagctgctgc tcctgcccct
     2101 gcctcagtcc cacagatatc ccagcccccc ttcttgttgg atgggctttc atcacagcct
     2161 ctcttcaatg atattgctgc aggcatcccc tccatcacag catacagtaa gaatggcttg
     2221 aagatagaat tcacctttga acggtcaaat accaacccca gtgtaacagt gataacgata
     2281 caggcctcca acagcacaga gctagatatg acggactttg ttttccaagc tgcagtacca
     2341 aagacattcc agctgcagct cttgtctcct agcagcagca ttgtcccagc atttaacacg
     2401 gggaccatca cacaagtcat taaagttctg aaccctcaga agcaacagct gcgaatgcgg
     2461 atcaagctta catataatca caagggctca gcaatgcaag atctagcaga ggtgaacaac
     2521 tttccccctc agtcctggca atgagggttt ggcaccattc tcattcttta tcccactcaa
     2581 tcaaaggaac tctgggaagg aggttgtgat tgctggcaag tcccccccaa ctgtaccacg
     2641 ggcatgagga gctgaagaga actgctgagg aggattttcc taaagttact gctgaccttg
     2701 aagcattgtt aaagactaat gtcctctcct ccactgttga ggctggctgc ttctggaggc
     2761 tactttgcac tcttcctctt ctcctttttc cgcacttctc cacccctccc acatttacag
     2821 ccagaatcaa cattccctgg gcccctgagg aaataagcag ctggtctgga ggagaggact
     2881 gcaatccatg gcgaaaaaac actcactttg tctctgcagc aaagagttgc cccttctttc
     2941 tactgttgtt tctctgtgga ctgggcaagg tggggtattt attcctcact agctgggtta
     3001 ccatcttcag gcacttttaa catctggcat tcggaatgga aatgtaataa tggacattag
     3061 ggagccctgc ctttttctac tggttccccc aatgtttgaa agaggcatta ggctcctggt
     3121 agccttttct gtgcattgct gtatacacac agacacacac atgtatgttt gttaccaaga
     3181 actggtcaga ccttgcgagt ttatttgtaa acactggaca gatggagtta aaaagagctt
     3241 ttgttgagat ttggcatgaa ggatatggtg ctctatttgt aatagaaact tccaaggctc
     3301 ttccagctcc cctttctcgc cattctttag ctgtagtcat gaatagtctc catgattttc
     3361 aaaattgatt ccctttaaag tgcaaaatgg tcaccttcta aaagatatat tcatagttat
     3421 taatgaccct attcccacca caaattttaa agtgctccta agcccataac ttgcctgttt
     3481 gaactatggt aatgggtgga agaggagttc accagtttca aagatcagac tctgtatcaa
     3541 aagtaccttt gcccttagga agagtgagta ttggagtcat cttatctatt actccaaacc
     3601 tcccttttta tttcttgagc ctggcttgga ccttggcatt ccgtttgaat tccttctaac
     3661 tggaacattt gtgttgtatc tgtaacactg gcactgaaat aaagaccaca cggttaaaga
     3721 aatctttcca tattgtactt tatggtgttg gagtgaagcc ttgtagcttc cataccccta
     3781 tgtcagagga ggtcttacgg acaccatagg gtaggaatag cctttcctca gtctgagaaa
     3841 ttggtctctt ttaaaagacg aatctcatga atattcacat caaagacttg agctttttaa
     3901 actagtgaga gtgccaagtg ctttttagaa aggacccata tgttatcaaa ctttgaaatt
     3961 gagttgctgg aatgaagtag aggtgactct ctctgtggta cacattgaat gtactatgta
     4021 tgttcaagta ttcaggcgcc atgtcttata tactgaagaa agaaaaagtg aggcccacct
     4081 tgctcttaca atgtttgcaa ttgttactgt attgaataca gtataatgac tactatggct
     4141 tcaatcttaa acctggaaac aaatatccct ttttttcccc ttcatttcac caagccttta
     4201 cttaaaatct tcagtgtctt gtcaaatcta gctctgtatc agatgctgga atattcctaa
     4261 catttgacaa actggagttg aactaaaggc tccacgggaa agtttctggt cttactagtg
     4321 tgtatgagca agatctgcta aaacttactc cactgggtaa atggttgact gagtcaagaa
     4381 caggataata tctcctgcat agttttcagt aatgtaagtg tggactagtg catatttcag
     4441 acaactgctc tgcctgtgca atgaaaaata gcctttaagg gtttctttgc agactgattt
     4501 cattggatgg atacttaatg ctgtgaaaca tgataggatt aacataatgt tggtggattt
     4561 cttgaataga atttgtctta acattcctct ttgtgtagag gctttatttt ctctcttata
     4621 tttgtagcta accagctcag gtttttttat ttgaactggg ttgaatctct gaagaaatct
     4681 gttcaagacc atgctataag acactgtcag ctaatggagc tgggaagggt ctactctgct
     4741 gacagagcat ttccttgggt gatcatagtt tcgaggtaga gtttatgatc attcatagct
     4801 ttgtctagaa ggagtaaaat atcatggcct taacacaaag ggtgctgcgt agaatatgaa
     4861 ttgattttgg aatcagaaca caagcaccat actgaaggac tagcagccaa ataactgcct
     4921 aggatactga tggttgtgaa gactgtttca aatgattgga tctttgaaag cttcagcgtg
     4981 ccttagtttc taggatcaga attagttttc ctctcacttg gccttgcagc taaatggaga
     5041 aatgtttcaa tttctttgaa tacttgcaca tttcaataat tcctttcccg agtataacca
     5101 ctcaaggggg agcaaatttg gatggattta cgacttcaca ggcattgtga ggaaagagca
     5161 ttttccaagg ctgttttgat aaccctgggg tgataagcag tgagccctca cacacttact
     5221 ttgacaattt cacatgcact tgtacttcat tatttccctc ttcaagagtc gtttctattc
     5281 tagtttctgc cccatcccgg ggaatcctaa aggagaatta attcatctaa gtaatctcaa
     5341 aaaactgtag gaagggtgct ctccctgaga agcttctccc acagtgcttt ggtgctgtta
     5401 ccttgaggtg gtttggacag tcacggaagt tttaggctgt gcatagtgat catctgttaa
     5461 ttttaaggtc tttatcattt aaagaaacat tcctcagtgt aacatttggg aggggattct
     5521 ttcctcttgc tagtttaaag gtgtgatttg tactccttgt ttgtcccatt catatatgaa
     5581 aatagacttt taaaactgtc caacactaat ggtttatata acatgcttcc catttttttt
     5641 atgtcgtaga aattggaagt tagggagtac tgctttcaag gttcaacttc attatcttct
     5701 gcattggaaa atatttgggc catgagaact aggggaaagg agtttgaatg tgtctatttt
     5761 tttctagtga atgtatttta accacagtgt cctaaactga gaaaactaga gaggaaaaag
     5821 tgggtgttca tgaactttgt agttgggaga gtggttttac atgtctgtgt attcatgact
     5881 ttgggagtgg gtaggatcat tggagagaga attgcacaga aagtcctgaa gtttaaaaca
     5941 cttttgacca gctttggctc gggagagtgg ggctgcttgt agaactggaa gtgaataact
     6001 ttttcaagca atatcagtga gtgggtccca tcgacagggt tccaggacct ggaacacttt
     6061 aacagaagga aatgccgaag cagcttgcac agttgcttta cagacttcca agaggctgat
     6121 tctggcttca agatggagcc ttggagttgg tttttttttt tttttttttc ttccctcaaa
     6181 gaacctgcgg ttgcgctttg tgtgttttgt ttttgttttc catttggggg ccccatggga
     6241 aagagcttct gaactctttc ctttatgaac tcccactgtg ttcctataaa ggcccttttc
     6301 tttcttagtg ttgtaagtta cattttcatt atgccccatc acatcttctt tactgtaaaa
     6361 atattaaaaa gctgtttcca agtgggacag ctaatgaagc tctaattatt gcagacatat
     6421 ttttgagatg taaaaaaaaa aatttaaagt taaatgataa gtcttagagg cgagtgagga
     6481 ataaaatgga tgtaaacatt tacatgggat gcattagaat tctgctgtgt gtactgtctt
     6541 ttggttgaaa caaattatga acagtgacta ataataaaaa gtcaataccc aatgatttaa
     6601 aa
//
