LOCUS       NM_000216               6265 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens anosmin 1 (ANOS1), mRNA.
ACCESSION   NM_000216
VERSION     NM_000216.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Chu,G., Li,P., Zhao,Q., He,R. and Zhao,Y.
  TITLE     Mutation spectrum of Kallmann syndrome: identification of five
            novel mutations across ANOS1 and FGFR1
  JOURNAL   Reprod Biol Endocrinol 21 (1), 23 (2023)
   PUBMED   36859276
  REMARK    GeneRIF: Mutation spectrum of Kallmann syndrome: identification of
            five novel mutations across ANOS1 and FGFR1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6265)
  AUTHORS   Zeng,W., Li,J., Wang,X., Jiang,F. and Men,M.
  TITLE     ANOS1 variants in a large cohort of Chinese patients with
            congenital hypogonadotropic hypogonadism
  JOURNAL   Zhong Nan Da Xue Xue Bao Yi Xue Ban 47 (7), 847-857 (2022)
   PUBMED   36039580
  REMARK    GeneRIF: ANOS1 variants in a large cohort of Chinese patients with
            congenital hypogonadotropic hypogonadism.', trans 'ANOS1.
REFERENCE   3  (bases 1 to 6265)
  AUTHORS   Louden,E.D., Poch,A., Kim,H.G., Ben-Mahmoud,A., Kim,S.H. and
            Layman,L.C.
  TITLE     Genetics of hypogonadotropic Hypogonadism-Human and mouse genes,
            inheritance, oligogenicity, and genetic counseling
  JOURNAL   Mol Cell Endocrinol 534, 111334 (2021)
   PUBMED   34062169
  REMARK    GeneRIF: Genetics of hypogonadotropic Hypogonadism-Human and mouse
            genes, inheritance, oligogenicity, and genetic counseling.
            Review article
REFERENCE   4  (bases 1 to 6265)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 6265)
  AUTHORS   Jiang,X., Li,D., Gao,Y., Zhang,X., Wang,X., Yang,Y. and Shen,Y.
  TITLE     A novel splice site variant in ANOS1 gene leads to Kallmann
            syndrome in three siblings
  JOURNAL   Gene 726, 144177 (2020)
   PUBMED   31669640
  REMARK    GeneRIF: The deleterious effect of this mutation on the
            transcriptional level of ANOS1 gene.
REFERENCE   6  (bases 1 to 6265)
  AUTHORS   Balasubramanian,R. and Crowley,W.F. Jr.
  TITLE     Isolated Gonadotropin-Releasing Hormone (GnRH) Deficiency
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301509
REFERENCE   7  (bases 1 to 6265)
  AUTHORS   del Castillo,I., Cohen-Salmon,M., Blanchard,S., Lutfalla,G. and
            Petit,C.
  TITLE     Structure of the X-linked Kallmann syndrome gene and its homologous
            pseudogene on the Y chromosome
  JOURNAL   Nat Genet 2 (4), 305-310 (1992)
   PUBMED   1303284
REFERENCE   8  (bases 1 to 6265)
  AUTHORS   Guioli,S., Incerti,B., Zanaria,E., Bardoni,B., Franco,B.,
            Taylor,K., Ballabio,A. and Camerino,G.
  TITLE     Kallmann syndrome due to a translocation resulting in an X/Y fusion
            gene
  JOURNAL   Nat Genet 1 (5), 337-340 (1992)
   PUBMED   1302031
REFERENCE   9  (bases 1 to 6265)
  AUTHORS   Legouis,R., Hardelin,J.P., Levilliers,J., Claverie,J.M.,
            Compain,S., Wunderle,V., Millasseau,P., Le Paslier,D., Cohen,D.,
            Caterina,D. et al.
  TITLE     The candidate gene for the X-linked Kallmann syndrome encodes a
            protein related to adhesion molecules
  JOURNAL   Cell 67 (2), 423-435 (1991)
   PUBMED   1913827
REFERENCE   10 (bases 1 to 6265)
  AUTHORS   Franco,B., Guioli,S., Pragliola,A., Incerti,B., Bardoni,B.,
            Tonlorenzi,R., Carrozzo,R., Maestrini,E., Pieretti,M.,
            Taillon-Miller,P., Brown,C.J., Willard,H.F., Lawrence,C., Graziella
            Persico,M., Camerino,G. and Ballabio,A.
  TITLE     A gene deleted in Kallmann's syndrome shares homology with neural
            cell adhesion and axonal path-finding molecules
  JOURNAL   Nature 353 (6344), 529-536 (1991)
   PUBMED   1922361
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC137426.1, CD642906.1,
            CN417843.1, DA809233.1, DB214183.1, AC006062.5, M97252.1 and
            AA678961.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_000216.3.
            
            Summary: Mutations in this gene cause the X-linked Kallmann
            syndrome. The encoded protein is similar in sequence to proteins
            known to function in neural cell adhesion and axonal migration. In
            addition, this cell surface protein is N-glycosylated and may have
            anti-protease activity. [provided by RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M97252.1, AH003768.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262648.8/ ENSP00000262648.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1300              BC137426.1         15-1314
            1301-1990           CD642906.1         9-698
            1991-2308           CN417843.1         358-675
            2309-2804           DA809233.1         48-543
            2805-3118           DB214183.1         236-549
            3119-4462           AC006062.5         47836-49179
            4463-6263           M97252.1           4512-6312
            6264-6265           AA678961.1         11-12               c
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xp22.31"
     gene            1..6265
                     /gene="ANOS1"
                     /gene_synonym="ADMLX; HH1; HHA; KAL; KAL1; KALIG-1; KMS;
                     WFDC19"
                     /note="anosmin 1"
                     /db_xref="GeneID:3730"
                     /db_xref="HGNC:HGNC:6211"
                     /db_xref="MIM:300836"
     exon            1..308
                     /gene="ANOS1"
                     /gene_synonym="ADMLX; HH1; HHA; KAL; KAL1; KALIG-1; KMS;
                     WFDC19"
                     /inference="alignment:Splign:2.1.0"
     CDS             102..2144
                     /gene="ANOS1"
                     /gene_synonym="ADMLX; HH1; HHA; KAL; KAL1; KALIG-1; KMS;
                     WFDC19"
                     /EC_number="3.4.21.4"
                     /note="Kallmann syndrome-1 sequence (anosmin-1); adhesion
                     molecule-like X-linked; Kallmann syndrome interval gene 1;
                     kallmann syndrome protein; WAP four-disulfide core domain
                     19"
                     /codon_start=1
                     /product="anosmin-1 precursor"
                     /protein_id="NP_000207.2"
                     /db_xref="CCDS:CCDS14130.1"
                     /db_xref="GeneID:3730"
                     /db_xref="HGNC:HGNC:6211"
                     /db_xref="MIM:300836"
                     /translation="MVPGVPGAVLTLCLWLAASSGCLAAGPGAAAARRLDESLSAGSV
                     QRARCASRCLSLQITRISAFFQHFQNNGSLVWCQNHKQCSKCLEPCKESGDLRKHQCQ
                     SFCEPLFPKKSYECLTSCEFLKYILLVKQGDCPAPEKASGFAAACVESCEVDNECSGV
                     KKCCSNGCGHTCQVPKTLYKGVPLKPRKELRFTELQSGQLEVKWSSKFNISIEPVIYV
                     VQRRWNYGIHPSEDDATHWQTVAQTTDERVQLTDIRPSRWYQFRVAAVNVHGTRGFTA
                     PSKHFRSSKDPSAPPAPANLRLANSTVNSDGSVTVTIVWDLPEEPDIPVHHYKVFWSW
                     MVSSKSLVPTKKKRRKTTDGFQNSVILEKLQPDCDYVVELQAITYWGQTRLKSAKVSL
                     HFTSTHATNNKEQLVKTRKGGIQTQLPFQRRRPTRPLEVGAPFYQDGQLQVKVYWKKT
                     EDPTVNRYHVRWFPEACAHNRTTGSEASSGMTHENYIILQDLSFSCKYKVTVQPIRPK
                     SHSKAEAVFFTTPPCSALKGKSHKPVGCLGEAGHVLSKVLAKPENLSASFIVQDVNIT
                     GHFSWKMAKANLYQPMTGFQVTWAEVTTESRQNSLPNSIISQSQILPSDHYVLTVPNL
                     RPSTLYRLEVQVLTPGGEGPATIKTFRTPELPPSSAHRSHLKHRHPHHYKPSPERY"
     exon            309..356
                     /gene="ANOS1"
                     /gene_synonym="ADMLX; HH1; HHA; KAL; KAL1; KALIG-1; KMS;
                     WFDC19"
                     /inference="alignment:Splign:2.1.0"
     exon            357..419
                     /gene="ANOS1"
                     /gene_synonym="ADMLX; HH1; HHA; KAL; KAL1; KALIG-1; KMS;
                     WFDC19"
                     /inference="alignment:Splign:2.1.0"
     exon            420..642
                     /gene="ANOS1"
                     /gene_synonym="ADMLX; HH1; HHA; KAL; KAL1; KALIG-1; KMS;
                     WFDC19"
                     /inference="alignment:Splign:2.1.0"
     exon            643..827
                     /gene="ANOS1"
                     /gene_synonym="ADMLX; HH1; HHA; KAL; KAL1; KALIG-1; KMS;
                     WFDC19"
                     /inference="alignment:Splign:2.1.0"
     exon            828..957
                     /gene="ANOS1"
                     /gene_synonym="ADMLX; HH1; HHA; KAL; KAL1; KALIG-1; KMS;
                     WFDC19"
                     /inference="alignment:Splign:2.1.0"
     exon            958..1163
                     /gene="ANOS1"
                     /gene_synonym="ADMLX; HH1; HHA; KAL; KAL1; KALIG-1; KMS;
                     WFDC19"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1308
                     /gene="ANOS1"
                     /gene_synonym="ADMLX; HH1; HHA; KAL; KAL1; KALIG-1; KMS;
                     WFDC19"
                     /inference="alignment:Splign:2.1.0"
     exon            1309..1455
                     /gene="ANOS1"
                     /gene_synonym="ADMLX; HH1; HHA; KAL; KAL1; KALIG-1; KMS;
                     WFDC19"
                     /inference="alignment:Splign:2.1.0"
     exon            1456..1550
                     /gene="ANOS1"
                     /gene_synonym="ADMLX; HH1; HHA; KAL; KAL1; KALIG-1; KMS;
                     WFDC19"
                     /inference="alignment:Splign:2.1.0"
     exon            1551..1722
                     /gene="ANOS1"
                     /gene_synonym="ADMLX; HH1; HHA; KAL; KAL1; KALIG-1; KMS;
                     WFDC19"
                     /inference="alignment:Splign:2.1.0"
     exon            1723..1943
                     /gene="ANOS1"
                     /gene_synonym="ADMLX; HH1; HHA; KAL; KAL1; KALIG-1; KMS;
                     WFDC19"
                     /inference="alignment:Splign:2.1.0"
     exon            1944..2085
                     /gene="ANOS1"
                     /gene_synonym="ADMLX; HH1; HHA; KAL; KAL1; KALIG-1; KMS;
                     WFDC19"
                     /inference="alignment:Splign:2.1.0"
     exon            2086..6265
                     /gene="ANOS1"
                     /gene_synonym="ADMLX; HH1; HHA; KAL; KAL1; KALIG-1; KMS;
                     WFDC19"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6244..6249
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANOS1"
                     /gene_synonym="ADMLX; HH1; HHA; KAL; KAL1; KALIG-1; KMS;
                     WFDC19"
                     /note="hexamer: AATAAA"
     polyA_site      6265
                     /gene="ANOS1"
                     /gene_synonym="ADMLX; HH1; HHA; KAL; KAL1; KALIG-1; KMS;
                     WFDC19"
                     /note="major polyA site"
ORIGIN      
        1 agtcgcggcg tctgcctggt cctcagcagt gcagccccgg cgcggagcag ggagcctcgg
       61 cccgcgcccg gcgccctcgc cctcgccctc gacccgcagc catggtgccc ggggtgcccg
      121 gcgcggtcct gaccctctgc ctctggctgg cggcctccag cggctgcctg gcggccggcc
      181 ccggcgcggc tgctgcgcgg cggctggacg agtcgctgtc tgccgggagc gtccagcgcg
      241 ctcgctgcgc ctccaggtgc ctgagcctgc agatcactcg catctccgcc ttcttccagc
      301 acttccagaa caatggttcc ctggtttggt gccagaatca caagcaatgt tctaagtgcc
      361 tggagccctg caaggaatca ggggacctga ggaaacacca gtgccaaagc ttttgtgagc
      421 ctctcttccc caagaagagc tacgaatgct tgaccagctg tgagttcctc aaatacatcc
      481 tgttggtgaa gcagggggac tgtccggctc ctgagaaagc cagtggattt gcggccgcct
      541 gtgttgaaag ctgcgaagtt gacaatgagt gctctggggt gaagaaatgt tgttcgaatg
      601 ggtgtggaca cacctgtcaa gtacccaaga ctctgtacaa aggtgtcccc ctgaagccca
      661 gaaaagagtt acgatttaca gaactgcagt ctggacagct ggaggttaag tggtcctcga
      721 aattcaatat ttctattgag cctgtgatct atgtggtaca aagaagatgg aattatggaa
      781 tccatcctag cgaagatgac gccactcact ggcagacagt ggcccagacc acagacgagc
      841 gagttcaact gactgacata agacccagcc gatggtacca gtttcgagtg gctgctgtga
      901 atgtgcatgg aactcgaggc ttcactgccc ccagcaaaca cttccgttct tccaaagatc
      961 catctgcccc accagcaccg gctaacctcc ggctggccaa ctccaccgtc aacagtgatg
     1021 ggagtgtgac cgtcactata gtttgggatc tccccgagga gccggacatc cctgtgcatc
     1081 attacaaggt cttttggagc tggatggtca gcagtaagtc tcttgtccca acaaagaaga
     1141 agcggagaaa gactacggat gggtttcaaa attctgtgat cctggagaaa ctccagccag
     1201 actgtgacta tgttgtggaa ttgcaagcca taacgtactg gggacagaca cggctgaaga
     1261 gtgcaaaggt gtcccttcac ttcacatcga cacatgcaac caacaacaaa gaacagcttg
     1321 tgaaaactag aaaaggtgga attcaaacac aactcccttt tcaaagacga cgacccactc
     1381 gcccgctgga agtcggagct cccttctatc aggatggcca actgcaagtt aaagtctact
     1441 ggaagaagac agaagatccc actgtcaacc gatatcatgt gcggtggttt cctgaagcgt
     1501 gtgcccacaa cagaacaacc ggatcagagg catcatctgg catgacccac gaaaattaca
     1561 taattcttca agatctgtca ttttcctgca agtataaggt gactgtccaa ccaatacggc
     1621 caaaaagtca ctccaaggca gaagctgttt tcttcactac tccaccatgc tctgctctta
     1681 aggggaagag ccacaagcct gttggctgcc tgggcgaagc aggtcatgtt ctttctaagg
     1741 tgctagctaa gcctgagaac ctttctgctt cattcatcgt ccaggatgtg aacatcaccg
     1801 gtcacttttc ttggaagatg gccaaggcca atctctatca gcccatgact gggtttcaag
     1861 tgacttgggc tgaggtcact acggaaagca gacagaacag cctacccaac agcattattt
     1921 cacagtccca gatcctgcct tccgatcatt atgtcctaac agtgcccaat ctgagaccat
     1981 ctactcttta ccgactggaa gtgcaagtgc tgaccccagg aggggagggg ccggccacca
     2041 tcaagacgtt ccggacgccg gagctcccac cctcttcagc acacagatct catcttaagc
     2101 atcgtcatcc acatcattac aagccttctc cagaaagata ctaaactgtt caaaaagatt
     2161 ttgtgaaatt gcacagatgt gtaagcttgt tgaacttcgg ccacgagaca tgcacacttc
     2221 cagaggcagt gggaactgct cagaggcccg gactctccta tgtgacttta gtgcaggaag
     2281 aacttctgtc aatcatggac gcatctggag acaagtgaga aacagtagat tggtgaagac
     2341 agacaccagt tccctacaag catggagaaa atgaagaata ggcctgttta atgctaaatt
     2401 ttgttttcat gtatggtgtc gctcatttct attgaattac aacagaactc agttttccct
     2461 gaatttggag caccaaactc cgccccaaaa aggagagtaa caaatacaca attcacacat
     2521 aacactaagc gtaaatctaa tcaataaaat atatttttga ctaaattatt gattcgatat
     2581 gaaaaatcaa ctaagattac acagctttgt ttttttgaat ctttcctaag atcattttta
     2641 tcctaggtga tttttaaatg aaaatgtgta atctaaaata taccagcgaa tttaaatcta
     2701 aaaatgctcc tactttaagt accttgtgct gctctttatg caaaggtaaa tcaaagttcc
     2761 ctctataaat tatgatttac aaaagacacc caagccagag gaactcaatg aaataagctg
     2821 ctaatcagat tttaccttgg agaaatgaaa attatttctt ggggatgcct tttaatattt
     2881 gatcctatta tgtgagagat tttcctgata tgttatctta tttatatttt cccttatttt
     2941 cctcaatgca gataatagct tttggtgcac ttttgtttca ccatctgaaa attcacaaaa
     3001 cttcttgctt caaatgaaaa aatcccaact attgagcatg tttaaatctt tgcagagatt
     3061 tgccttttct taatcaaaga aaggtctttg tgtgctagaa tattattggt aatgttttaa
     3121 aaattccttt gattgataga gaaggacagt tatttgcatt taattcaccc atatgctttc
     3181 aaatctagta tatcttactt tttggaaatg ttttatgcta caaattagtg ccttgtagca
     3241 tgaacttaag tcaaaacgtg ttatcaatat agagtgttgc agtgtatatt gtaacaacct
     3301 aaaacgcaga gaagtttaat ttaatactgt tttttttctt gaaggaatac tcacatacat
     3361 ggtttgaaat gtgcatagat atgcatgtct atataattat aaatgcatgt gtatatatat
     3421 gcaaatatat gtacatatac atgtatatac acacagacac atgcatatac atgaatatac
     3481 cttgagcatg aatccctgga gaaatcgttt tcgtagctca ccaatggtga gtaaagatac
     3541 agctctttta aaggtcataa ggataatata ttttccccat caatgctgat tctgagaaaa
     3601 gagcaattta tcaaaattaa acactgtaaa agaaaggtgt ccatatgtct ttacctacct
     3661 aagtaaaaca ggaagaaaat cagtaacatt atccttaggt tttgacaatg gtacttgctt
     3721 cttgttgttt tattgtttcc tgaattcatg cagatgcctg gccattcctg ggaagagtgg
     3781 ataactcaga agtcactgta ctccacagag cctcactgca gtgtctaaag gtagatgcaa
     3841 attaaaatgc agggaaaata acttttctga tgttgatgca tgtctttggg aaacacattt
     3901 ataaacatgg atacctgata atagatattg aaacccattt cctgtgtgtt aaaatattta
     3961 aaaagtggat attccaggaa tgttttgcag ctttgtacaa gtaacataaa ttggacacct
     4021 cagaatgaaa gttcatgttg gttctgaatg gttcactgca gctcctgtca caagctggga
     4081 tggatttatc acattgagtt atgaaattac ctggttctaa gaatttttga gtggcaaaaa
     4141 tagaaaacaa tcttcatttg aaaacatccc taagcttgaa taaatggata ccatagatag
     4201 cttctctttt ttattctggt gtcattacca gcatctgaat ttcaagttct taaaatttca
     4261 aaaattaaaa tttttcatta ttagctatcc atttatcttt tacatgaact tgtcatgaac
     4321 aaattcaaat gtttatgcca gcaaattttt gtactgttgc atagttaaaa atgctgggag
     4381 tctctgcata gatacaaaat attattaaat tattacataa atttaatttt ataaaattta
     4441 atcatgcttc ttttgtctgg gtaatagaca ttggacagat atttttagtt cagatggtga
     4501 ttctgaagct tacatctccc ttaaaaaaat ctaaagcagc tcttatgggc ttctaatttt
     4561 aatataaata aataatttaa attttattgg tgttattgga agaaaaatgc tattaatggg
     4621 ctaataaaaa acatgtgttt ctcttatgga ttttaataag ctccagtatt attcaaatga
     4681 tcaaaaatat agttataatt ttttgaattt taaaaatgtg attgctctaa taaagaataa
     4741 aatctatgct ttttaacaaa catagttttg gtgcctaatt ctgtaatatg ttttattgaa
     4801 attagattca tttctctaat gtgagaaaaa tatatccagt aatagtattg actgtttaaa
     4861 aaattgagct catcaaaaat attgtcatca aatacaggtg gttaatctga catacattgc
     4921 agttacatgc attattttta tttacaacat ttgctcctta atgatgaatt tatctgtgtt
     4981 accctgtttt tctacctgga actccataga atgatgtttg caaaccaaca tgtgctcttt
     5041 tcagtcattc actgttttaa tatgacatgg tagagaagat aaggtttatg gcaggtaatt
     5101 ttttgtaatg tgtattaaac gaagttcaaa gattagaaat acatctgtgt cctgaaaacc
     5161 ttagatacat agccgactgt atacagaggt tcatctcaac ctcaacacta ttgacttttg
     5221 gggctggata gttctctgtt gtgggggttt gtcttgtgca ctgtaggttt ttagtagcat
     5281 ccacactttc tcctcaccag atgccagttg caccctcccc caagttgaga caaccaaaaa
     5341 tgtctccaga tattgccagc taccccttga gggatggtac ctctggttga gaaccattgc
     5401 tagagaatga tctttactga atttgccctt tataagaaac ccagtgaatt tctagagcaa
     5461 gtcccaaaaa ctaagggaca gctaagaagt tattatggtt gacttcaaag gcctaaactg
     5521 tgttttttat gtccactaaa caacttgatt aaaagacgga attttgactc gtgtctgtat
     5581 catacaagta caaatactaa ttttgcccta tgtatccgta aatgtcattt gtgattttga
     5641 cttatttatt taatgccctt tcttatgccg tgggttttca agtttactca tttctatggt
     5701 tgcaaataac tctaaaactt attatataaa ctttcatatt ataggcagaa cacaatggct
     5761 aaatatctgt tgcatgtact ttaaagttta ttataaaata taaacagata tataaagatg
     5821 ttgactctta cctgtgattt tgcatggtca gactcggtgt caggtacgga gaggattctc
     5881 atgactgtct tacctctact gaatattcta gtgagttata tgatttacgg agtgattaac
     5941 agaggtctat ataaagttac ttttcccctt tacttaatta tattgtagtg tgcagataac
     6001 aaaactgcta ccttctcatc caagtggtct gtagaattca tgtcccttac agtggtcatt
     6061 taaagtcaat atttatttat gtatgtaata aaaaaagttg gatttttgtg tatgtctgtc
     6121 acattattta gagagaagta atcttgtaaa aatgttttgt aaaaaacaaa aaagtattgt
     6181 aaatagtctt gatattctgt gactcattat tttcatgtta gagtttgtac atactggttc
     6241 aataataaag tatccttaaa ccaga
//
