LOCUS       NM_001370694            4560 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens anoctamin 7 (ANO7), transcript variant 1, mRNA.
ACCESSION   NM_001370694 NM_001001891
VERSION     NM_001370694.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Metsala,O., Wahlstrom,G., Taimen,P., Kellokumpu-Lehtinen,P.L. and
            Schleutker,J.
  TITLE     Transcripts of the Prostate Cancer-Associated Gene ANO7 Are
            Retained in the Nuclei of Prostatic Epithelial Cells
  JOURNAL   Int J Mol Sci 24 (2), 1052 (2023)
   PUBMED   36674564
  REMARK    GeneRIF: Transcripts of the Prostate Cancer-Associated Gene ANO7
            Are Retained in the Nuclei of Prostatic Epithelial Cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Wahlstrom,G., Heron,S., Knuuttila,M., Kaikkonen,E., Tulonen,N.,
            Metsala,O., Lof,C., Ettala,O., Bostrom,P.J., Taimen,P., Poutanen,M.
            and Schleutker,J.
  TITLE     The variant rs77559646 associated with aggressive prostate cancer
            disrupts ANO7 mRNA splicing and protein expression
  JOURNAL   Hum Mol Genet 31 (12), 2063-2077 (2022)
   PUBMED   35043958
  REMARK    GeneRIF: The variant rs77559646 associated with aggressive prostate
            cancer disrupts ANO7 mRNA splicing and protein expression.
REFERENCE   3  (bases 1 to 4560)
  AUTHORS   Marx,A., Koopmann,L., Hoflmayer,D., Buscheck,F., Hube-Magg,C.,
            Steurer,S., Eichenauer,T., Clauditz,T.S., Wilczak,W., Simon,R.,
            Sauter,G., Izbicki,J.R., Huland,H., Heinzer,H., Graefen,M.,
            Haese,A., Schlomm,T., Bernreuther,C., Lebok,P. and Bonk,S.
  TITLE     Reduced anoctamin 7 (ANO7) expression is a strong and independent
            predictor of poor prognosis in prostate cancer
  JOURNAL   Cancer Biol Med 18 (1), 245-255 (2021)
   PUBMED   33628598
  REMARK    GeneRIF: Reduced anoctamin 7 (ANO7) expression is a strong and
            independent predictor of poor prognosis in prostate cancer.
REFERENCE   4  (bases 1 to 4560)
  AUTHORS   Kaikkonen,E., Takala,A., Pursiheimo,J.P., Wahlstrom,G. and
            Schleutker,J.
  TITLE     The interactome of the prostate-specific protein Anoctamin 7
  JOURNAL   Cancer Biomark 28 (1), 91-100 (2020)
   PUBMED   32176628
  REMARK    GeneRIF: The interactome of the prostate-specific protein Anoctamin
            7.
REFERENCE   5  (bases 1 to 4560)
  AUTHORS   Kaikkonen,E., Rantapero,T., Zhang,Q., Taimen,P., Laitinen,V.,
            Kallajoki,M., Jambulingam,D., Ettala,O., Knaapila,J., Bostrom,P.J.,
            Wahlstrom,G., Sipeky,C., Pursiheimo,J.P., Tammela,T.,
            Kellokumpu-Lehtinen,P.L., Fey,V., Maehle,L., Wiklund,F., Wei,G.H.
            and Schleutker,J.
  CONSRTM   PRACTICAL Consortium
  TITLE     ANO7 is associated with aggressive prostate cancer
  JOURNAL   Int J Cancer 143 (10), 2479-2487 (2018)
   PUBMED   30157291
  REMARK    GeneRIF: ANO7 genotypes correlate with expression and biochemical
            relapse, suggesting that ANO7 is a potential PrCa susceptibility
            gene and that its elevated expression correlates with disease
            severity and outcome.
REFERENCE   6  (bases 1 to 4560)
  AUTHORS   Das,S., Hahn,Y., Walker,D.A., Nagata,S., Willingham,M.C.,
            Peehl,D.M., Bera,T.K., Lee,B. and Pastan,I.
  TITLE     Topology of NGEP, a prostate-specific cell:cell junction protein
            widely expressed in many cancers of different grade level
  JOURNAL   Cancer Res 68 (15), 6306-6312 (2008)
   PUBMED   18676855
  REMARK    GeneRIF: NGEP is a glycoprotein with predicted glycosylation sites
            at N809 and N824. When these residues were converted to glutamine,
            glycosylation was abolished, confirming that the residues are
            extracellular
REFERENCE   7  (bases 1 to 4560)
  AUTHORS   Kiessling,A., Weigle,B., Fuessel,S., Ebner,R., Meye,A.,
            Rieger,M.A., Schmitz,M., Temme,A., Bachmann,M., Wirth,M.P. and
            Rieber,E.P.
  TITLE     D-TMPP: a novel androgen-regulated gene preferentially expressed in
            prostate and prostate cancer that is the first characterized member
            of an eukaryotic gene family
  JOURNAL   Prostate 64 (4), 387-400 (2005)
   PUBMED   15761874
  REMARK    GeneRIF: description of the novel prostate-restricted molecule
            D-TMPP widely expressed in prostate cancer tissues
REFERENCE   8  (bases 1 to 4560)
  AUTHORS   Katoh,M. and Katoh,M.
  TITLE     Characterization of human TMEM16G gene in silico
  JOURNAL   Int J Mol Med 14 (4), 759-764 (2004)
   PUBMED   15375614
  REMARK    GeneRIF: Complete coding sequence of TMEM16G cDNA was determined by
            assembling 25 exons of TMEM16G gene
REFERENCE   9  (bases 1 to 4560)
  AUTHORS   Bera,T.K., Das,S., Maeda,H., Beers,R., Wolfgang,C.D., Kumar,V.,
            Hahn,Y., Lee,B. and Pastan,I.
  TITLE     NGEP, a gene encoding a membrane protein detected only in prostate
            cancer and normal prostate
  JOURNAL   Proc Natl Acad Sci U S A 101 (9), 3059-3064 (2004)
   PUBMED   14981236
  REMARK    GeneRIF: expressed only in prostate and prostate camcer is a
            promising target for the antibody-based therapies of prostate
            cancer
REFERENCE   10 (bases 1 to 4560)
  AUTHORS   Walker,M.G., Volkmuth,W., Sprinzak,E., Hodgson,D. and Klingler,T.
  TITLE     Prediction of gene function by genome-scale expression analysis:
            prostate cancer-associated genes
  JOURNAL   Genome Res 9 (12), 1198-1203 (1999)
   PUBMED   10613842
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC005237.2, KF456776.1,
            AC111201.6 and AC104841.6.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Aug 30, 2019 this sequence version replaced NM_001370694.1.
            
            Summary: This prostate-specific gene encodes a cytoplasmic protein,
            as well as a polytopic membrane protein which may serve as a target
            in prostate cancer diagnosis and immunotherapy. Alternative
            splicing results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Aug 2011].
            
            Transcript Variant: This variant (1, also known as NGEP-L)
            represents the longer transcript and encodes the longer isoform
            (isoform 1, also known as NGEP long).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY617079.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2142586
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: lack of evidence for use of
                                                upstream AUG
            MANE Ensembl match               :: ENST00000674324.2/
                                                ENSP00000501393.1
            RefSeq Select criteria           :: based on manual assertion,
                                                conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-90                AC005237.2         156644-156733
            91-205              AC005237.2         158024-158138
            206-263             AC005237.2         159161-159218
            264-406             AC005237.2         163670-163812
            407-514             AC005237.2         167283-167390
            515-651             AC005237.2         168056-168192
            652-709             AC005237.2         169265-169322
            710-820             AC005237.2         170161-170271
            821-971             KF456776.1         1-151               c
            972-986             AC005237.2         171451-171465
            987-1059            AC005237.2         172832-172904
            1060-1077           AC111201.6         1-18
            1078-1174           AC111201.6         2637-2733
            1175-1318           AC111201.6         4348-4491
            1319-1456           AC111201.6         4561-4698
            1457-1555           AC111201.6         5358-5456
            1556-1658           AC111201.6         5531-5633
            1659-1770           AC111201.6         7157-7268
            1771-1825           AC111201.6         7635-7689
            1826-1923           AC111201.6         9868-9965
            1924-2069           AC111201.6         11156-11301
            2070-2275           AC104841.6         131738-131943       c
            2276-2418           AC104841.6         131248-131390       c
            2419-2509           AC104841.6         126353-126443       c
            2510-2629           AC104841.6         125848-125967       c
            2630-2680           AC104841.6         125674-125724       c
            2681-4560           AC104841.6         123653-125532       c
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q37.3"
     gene            1..4560
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /note="anoctamin 7"
                     /db_xref="GeneID:50636"
                     /db_xref="HGNC:HGNC:31677"
                     /db_xref="MIM:605096"
     exon            1..90
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            91..205
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     CDS             98..2737
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /note="isoform 1 is encoded by transcript variant 1;
                     prostate cancer associated protein 5; transmembrane
                     protein 16G; Dresden-transmembrane protein of the
                     prostate"
                     /codon_start=1
                     /product="anoctamin-7 isoform 1"
                     /protein_id="NP_001357623.1"
                     /db_xref="CCDS:CCDS33423.2"
                     /db_xref="GeneID:50636"
                     /db_xref="HGNC:HGNC:31677"
                     /db_xref="MIM:605096"
                     /translation="MLRRRAQEEDSTVLIDVSPPEAEKRGSYGSTAHASEPGGQQAAA
                     CRAGSPAKPRIADFVLVWEEDLKLDRQQDSAARDRTDMHRTWRETFLDNLRAAGLCVD
                     QQDVQDGNTTVHYALLSASWAVLCYYAEDLRLKLPLQELPNQASNWSAGLLAWLGIPN
                     VLLEVVPDVPPEYYSCRFRVNKLPRFLGSDNQDTFFTSTKRHQILFEILAKTPYGHEK
                     KNLLGIHQLLAEGVLSAAFPLHDGPFKTPPEGPQAPRLNQRQVLFQHWARWGKWNKYQ
                     PLDHVRRYFGEKVALYFAWLGFYTGWLLPAAVVGTLVFLVGCFLVFSDIPTQELCGSK
                     DSFEMCPLCLDCPFWLLSSACALAQAGRLFDHGGTVFFSLFMALWAVLLLEYWKRKSA
                     TLAYRWDCSDYEDTEERPRPQFAASAPMTAPNPITGEDEPYFPERSRARRMLAGSVVI
                     VVMVAVVVMCLVSIILYRAIMAIVVSRSGNTLLAAWASRIASLTGSVVNLVFILILSK
                     IYVSLAHVLTRWEMHRTQTKFEDAFTLKVFIFQFVNFYSSPVYIAFFKGRFVGYPGNY
                     HTLFGVRNEECAAGGCLIELAQELLVIMVGKQVINNMQEVLIPKLKGWWQKFRLRSKK
                     RKAGASAGASQGPWEDDYELVPCEGLFDEYLEMVLQFGFVTIFVAACPLAPLFALLNN
                     WVEIRLDARKFVCEYRRPVAERAQDIGIWFHILAGLTHLAVISNAFLLAFSSDFLPRA
                     YYRWTRAHDLRGFLNFTLARAPSSFAAAHNRTCRYRAFRDDDGHYSQTYWNLLAIRLA
                     FVIVFEHVVFSVGRLLDLLVPDIPESVEIKVKREYYLAKQALAENEVLFGTNGTKDEQ
                     PEGSELSSHWTPFTVPKASQLQQ"
     exon            206..263
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            264..406
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            407..514
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            515..651
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            652..709
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            710..820
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            821..986
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1077
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            1078..1174
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            1175..1318
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            1319..1456
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            1457..1555
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            1556..1658
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            1659..1770
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            1771..1825
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            1826..1923
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            1924..2069
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            2070..2275
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            2276..2418
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            2419..2509
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            2510..2629
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            2630..2680
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     exon            2681..4560
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      3768
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
                     /note="major polyA site"
     polyA_site      4199
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
     polyA_site      4522
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
     polyA_site      4560
                     /gene="ANO7"
                     /gene_synonym="D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP;
                     PCANAP5; PCANAP5L; TMEM16G"
ORIGIN      
        1 agtgaggacg ggactctact gccgagacca ggctcacgct gagaggtggg ccatgacctc
       61 cgagacctct tccggaagcc actgtgccag gagcaggatg ctgcggcgac gggcccagga
      121 agaggacagc accgtcctga tcgatgtgag cccccctgag gcagagaaga ggggctctta
      181 cgggagcaca gcccacgcct cggagccagg tggacagcaa gcggccgcct gcagagctgg
      241 gagtcctgcc aagccccgga tcgcagactt cgtcctcgtt tgggaggagg acctgaagct
      301 agacaggcag caggacagtg ccgcccggga cagaacagac atgcacagga cctggcggga
      361 gacttttctg gataatcttc gtgcggctgg gctgtgtgta gaccagcagg acgtccagga
      421 cgggaacacc acagtgcact acgccctcct cagcgcctcc tgggctgtgc tctgctacta
      481 cgccgaagac ctgcgcctga agctgccctt gcaggagtta cccaaccagg cctccaactg
      541 gtcggccggc ctgctggcat ggctgggcat ccccaacgtc ctgctggagg ttgtgccaga
      601 cgtacccccc gagtactact cctgccggtt cagagtgaac aagctgccac gcttcctcgg
      661 gagtgacaac caggacacct tcttcacaag caccaagagg caccaaattc tgtttgagat
      721 cctggccaag accccgtatg gccacgagaa gaaaaacctg cttgggatcc accagctgct
      781 ggcagagggt gtcctcagtg ccgccttccc cctgcatgac ggccccttca agacgccccc
      841 agagggcccg caggctccac gcctcaacca gcgccaagtc cttttccagc actgggcgcg
      901 ctggggcaag tggaacaagt accagcccct ggaccacgtg cgcaggtact tcggggagaa
      961 ggtggccctc tacttcgcct ggctcgggtt ttacacaggc tggctcctgc cagcggcagt
     1021 ggtgggcaca ctggtgttcc tggtgggctg cttcctggtg ttctcagaca tacccacgca
     1081 ggaactgtgt ggcagcaagg acagcttcga gatgtgccca ctttgcctcg actgcccttt
     1141 ctggctgctc tccagcgcct gtgccctggc ccaggccggc cggctgttcg accacggcgg
     1201 caccgtgttc ttcagcttgt tcatggcact gtgggccgtg ctgctgctgg agtactggaa
     1261 gcggaagagc gccacgctgg cctaccgctg ggactgctct gactacgagg acactgagga
     1321 gaggcctcgg ccccagtttg ccgcctcagc ccccatgaca gccccgaacc ccatcacggg
     1381 tgaggacgag ccctacttcc ctgagaggag ccgcgcgcgc cgcatgctgg ccggctctgt
     1441 ggtgatcgtg gtgatggtgg ccgtggtggt catgtgcctc gtgtctatca tcctgtaccg
     1501 tgccatcatg gccatcgtgg tgtccaggtc gggcaacacc cttctcgcag cctgggcctc
     1561 tcgcatcgcc agcctcacgg ggtctgtagt gaacctcgtc ttcatcctca tcctctccaa
     1621 gatctatgta tccctggccc acgtcctgac acgatgggaa atgcaccgca cccagaccaa
     1681 gttcgaggac gccttcaccc tcaaggtgtt catcttccag ttcgtcaact tctactcctc
     1741 acccgtctac attgccttct tcaagggcag gtttgtggga tacccaggca actaccacac
     1801 cttgtttgga gtccgcaatg aggagtgcgc ggctggaggc tgcctgatcg agctggcaca
     1861 ggagctcctg gtcatcatgg tgggcaagca ggtcatcaac aacatgcagg aggtcctcat
     1921 cccgaagcta aagggctggt ggcagaagtt ccggcttcgc tccaagaaga ggaaggcggg
     1981 agcttctgca ggggctagcc aggggccctg ggaggacgac tatgagcttg tgccctgtga
     2041 gggtctgttt gacgagtacc tggaaatggt gctgcagttc ggcttcgtca ccatcttcgt
     2101 ggccgcctgt ccgctcgcgc cgctcttcgc cctgctcaac aactgggtgg agatccgctt
     2161 ggacgcgcgc aagttcgtct gcgagtaccg gcgcccggtg gccgagcgcg cccaggacat
     2221 cggcatctgg ttccacatcc tggcgggcct cacgcacctg gcggtcatca gcaacgcctt
     2281 cctcctggcc ttctcgtccg acttcctgcc gcgcgcctac taccggtgga cccgcgccca
     2341 cgacctgcgc ggcttcctca acttcacgct ggcgcgagcc ccgtcctcct tcgccgccgc
     2401 gcacaaccgc acgtgcaggt atcgggcttt ccgggatgac gatggacatt attcccagac
     2461 ctactggaat cttcttgcca tccgcctggc cttcgtcatt gtgtttgagc atgtggtttt
     2521 ctccgttggc cgcctcctgg acctcctggt gcctgacatc ccagagtctg tggagatcaa
     2581 agtgaagcgg gagtactacc tggctaagca ggcactggct gagaatgagg ttctttttgg
     2641 aacgaacgga acaaaggatg agcagcccga gggctcagag ctcagctccc actggacacc
     2701 cttcacggtt cccaaggcca gccagctgca gcagtgacgc ctggaaggac atctggtggt
     2761 ccttagggga gtggcccctc ctgagccctg cgagcagcgt ccttttcctc ttccctcagg
     2821 cagcggctgt gtgaaccgct ggctgctgtt gtgcctcatc tctgggcaca ttgcctgctt
     2881 ccccccagcg ccggcttctc tcctcagagc gcctgtcact ccatccccgg cagggaggga
     2941 ccgtcagctc acaaggccct ctttgtttcc tgctcccaga cataagccca aggggcccct
     3001 gcacccaagg gaccctgtcc ctcggtggcc tccccaggcc cctggacacg acagttctcc
     3061 tcaggcaggt gggctttgtg gtcctcgccg cccctggcca catcgccctc tcctcttaca
     3121 cctggtgacc ttcgaatgtt tcagagcgca gggccgttct ccctcgtgtc ctctggaccc
     3181 acccgcccct tcctgccctg tttgcgcagg gacatcaccc acatgcccca gctctcggac
     3241 cctgcagctc tgtgtcccag gccacagcaa aggtctgttg aacccctccc tccattccca
     3301 gttatctggg tcctctggat tcttctgttt cttgaatcag gctctgcttt ccccctagcc
     3361 actacaggca gcctctgaca gtgccgcttt acttgcattc tgcagcaatt acatgtgtcc
     3421 ttttgatcct tgcccaactt ccctccctct cccagctcct ggcccctggc ccagggcccc
     3481 tcttgctgtt tttacctctg ttccttgggg cctagtaccc agcaagcacc caaatggggg
     3541 aggttttggg atgagaggag gaaacgtgta tacctgtaac atctggtggc tcttccccca
     3601 gaagtttgtg ttcatacata attgttttcc acgctggatc ataatgtgac gtgcagttct
     3661 gccctgtgct ggggagccac atgaagcttc ccctggctaa cttgctaccc cgcagcaatc
     3721 ccagtgtggc cgtctgcttg ctaaaaaatg gatctgtgct catctgtatt gatgtccttg
     3781 gagttctaca agtggaactt aagtgtcaaa aagaatatgt ggtttttagc tgagcgtggt
     3841 ggctcacacc tgtaatccca ggactttggg aggctgaggc aggaggatta caaggtcagg
     3901 agttcgggac tagcctgtcc aacatggtga aaccctgtct ttactaaaaa tgcaaaaatt
     3961 attctgggtg tggtggcggg catctgtaat cccagctatt tgggaggctg aggcaggaga
     4021 atcgtttgaa cccgggaggc ggaggttgta gtgagctgag atctcaacac tgcactacag
     4081 cctgggcgac agagcaagac tccgtctcgg gaacacacac acacaaaaag aatatgtggt
     4141 tttaatgtgc tttgatgagt actgccaaac ttactccaca gaaaaggcct ctttctgaac
     4201 atcctcgcct gcgttctatt tcacccaccg tgatgcctgg cctgagggcg gcgtgcttgc
     4261 ttgtagcatt tcttgaggat ttgctacttg ttcacctgcc tcttaggagc actgtgccct
     4321 gcctccatgg aagggctctt ccggcaggga tgcaggctca cagcgccctg gggctggaca
     4381 ccaccggccg gagcatggcg gacagcacac acggcccggg gcgggaacct tggaaacttt
     4441 acacagatgg ggagctcagc cattccacgt gtgctttcgc tcagcacaat gcttactaca
     4501 aacccacgtg tacttccttc cagctggttg ctttttattg ttgctgtctt aaactccaaa
//
