LOCUS       NM_213599               6742 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens anoctamin 5 (ANO5), transcript variant 1, mRNA.
ACCESSION   NM_213599
VERSION     NM_213599.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6742)
  AUTHORS   Zhou,Z., Zhang,Y., Zhu,L., Cui,Y., Gao,Y. and Zhou,C.X.
  TITLE     Familial gigantiform cementoma with recurrent ANO5 p.Cys356Tyr
            mutations: Clinicopathological and genetic study with literature
            review
  JOURNAL   Mol Genet Genomic Med 12 (1), e2277 (2024)
   PUBMED   37649308
  REMARK    GeneRIF: Familial gigantiform cementoma with recurrent ANO5
            p.Cys356Tyr mutations: Clinicopathological and genetic study with
            literature review.
            Review article
REFERENCE   2  (bases 1 to 6742)
  AUTHORS   de Bruyn,A., Montagnese,F., Holm-Yildiz,S., Scharff Poulsen,N.,
            Stojkovic,T., Behin,A., Palmio,J., Jokela,M., De Bleecker,J.L., de
            Visser,M., van der Kooi,A.J., Ten Dam,L., Dominguez Gonzalez,C.,
            Maggi,L., Gallone,A., Kostera-Pruszczyk,A., Macias,A.,
            Lusakowska,A., Nedkova,V., Olive,M., Alvarez-Velasco,R.,
            Wanschitz,J., Paradas,C., Mavillard,F., Querin,G.,
            Fernandez-Eulate,G., Quinlivan,R., Walter,M.C., Depuydt,C.E.,
            Udd,B., Vissing,J., Schoser,B. and Claeys,K.G.
  TITLE     Anoctamin-5 related muscle disease: clinical and genetic findings
            in a large European cohort
  JOURNAL   Brain 146 (9), 3800-3815 (2023)
   PUBMED   36913258
  REMARK    GeneRIF: Anoctamin-5 related muscle disease: clinical and genetic
            findings in a large European cohort.
REFERENCE   3  (bases 1 to 6742)
  AUTHORS   Fukami,T., Shiozaki,A., Kosuga,T., Kudou,M., Shimizu,H., Ohashi,T.,
            Arita,T., Konishi,H., Komatsu,S., Kubota,T., Fujiwara,H.,
            Okamoto,K., Kishimoto,M., Morinaga,Y., Konishi,E. and Otsuji,E.
  TITLE     Anoctamin 5 regulates the cell cycle and affects prognosis in
            gastric cancer
  JOURNAL   World J Gastroenterol 28 (32), 4649-4667 (2022)
   PUBMED   36157935
  REMARK    GeneRIF: Anoctamin 5 regulates the cell cycle and affects prognosis
            in gastric cancer.
REFERENCE   4  (bases 1 to 6742)
  AUTHORS   Hartzell,H.C., Yu,K., Xiao,Q., Chien,L.T. and Qu,Z.
  TITLE     Anoctamin/TMEM16 family members are Ca2+-activated Cl- channels
  JOURNAL   J Physiol 587 (Pt 10), 2127-2139 (2009)
   PUBMED   19015192
  REMARK    Review article
REFERENCE   5  (bases 1 to 6742)
  AUTHORS   Jarry,J., Rioux,M.F., Bolduc,V., Robitaille,Y., Khoury,V.,
            Thiffault,I., Tetreault,M., Loisel,L., Bouchard,J.P. and Brais,B.
  TITLE     A novel autosomal recessive limb-girdle muscular dystrophy with
            quadriceps atrophy maps to 11p13-p12
  JOURNAL   Brain 130 (Pt 2), 368-380 (2007)
   PUBMED   17008331
REFERENCE   6  (bases 1 to 6742)
  AUTHORS   Katoh,M. and Katoh,M.
  TITLE     GDD1 is identical to TMEM16E, a member of the TMEM16 family
  JOURNAL   Am J Hum Genet 75 (5), 927-8 (2004)
   PUBMED   15457408
REFERENCE   7  (bases 1 to 6742)
  AUTHORS   Tsutsumi,S., Kamata,N., Vokes,T.J., Maruoka,Y., Nakakuki,K.,
            Enomoto,S., Omura,K., Amagasa,T., Nagayama,M., Saito-Ohara,F.,
            Inazawa,J., Moritani,M., Yamaoka,T., Inoue,H. and Itakura,M.
  TITLE     The novel gene encoding a putative transmembrane protein is mutated
            in gnathodiaphyseal dysplasia (GDD)
  JOURNAL   Am J Hum Genet 74 (6), 1255-1261 (2004)
   PUBMED   15124103
REFERENCE   8  (bases 1 to 6742)
  AUTHORS   Katoh,M. and Katoh,M.
  TITLE     Identification and characterization of TMEM16E and TMEM16F genes in
            silico
  JOURNAL   Int J Oncol 24 (5), 1345-1349 (2004)
   PUBMED   15067359
REFERENCE   9  (bases 1 to 6742)
  AUTHORS   Penttila,S., Vihola,A., Palmio,J. and Udd,B.
  TITLE     ANO5 Muscle Disease
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   23193613
REFERENCE   10 (bases 1 to 6742)
  AUTHORS   Pegoraro,E. and Hoffman,E.P.
  TITLE     Limb-Girdle Muscular Dystrophy Overview - RETIRED CHAPTER, FOR
            HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301582
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC107886.2, AB125267.1,
            AK125046.1, AC104009.7, AL833271.1 and BU742779.1.
            
            On Dec 4, 2019 this sequence version replaced NM_213599.2.
            
            Summary: This gene encodes a member of the anoctamin family of
            transmembrane proteins. The encoded protein is likely a calcium
            activated chloride channel. Mutations in this gene have been
            associated with gnathodiaphyseal dysplasia. Alternatively spliced
            transcript variants have been described. [provided by RefSeq, Nov
            2009].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL833271.1, AB125267.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000324559.9/ ENSP00000315371.9
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-91                AC107886.2         52081-52171
            92-674              AB125267.1         1-583
            675-675             AC107886.2         80179-80179
            676-3238            AB125267.1         585-3147
            3239-3262           AK125046.1         1049-1072
            3263-3263           AC104009.7         32248-32248
            3264-3645           AK125046.1         1073-1454
            3646-3899           AL833271.1         3574-3827
            3900-4435           AK125046.1         1709-2244
            4436-5210           AL833271.1         4364-5138
            5211-5646           AK125046.1         3015-3450
            5647-6151           AL833271.1         5575-6079
            6152-6742           BU742779.1         16-606              c
FEATURES             Location/Qualifiers
     source          1..6742
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p14.3"
     gene            1..6742
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /note="anoctamin 5"
                     /db_xref="GeneID:203859"
                     /db_xref="HGNC:HGNC:27337"
                     /db_xref="MIM:608662"
     exon            1..448
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /inference="alignment:Splign:2.1.0"
     CDS             409..3150
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /note="isoform a is encoded by transcript variant 1;
                     transmembrane protein 16E; integral membrane protein GDD1;
                     gnathodiaphyseal dysplasia 1 protein"
                     /codon_start=1
                     /product="anoctamin-5 isoform a"
                     /protein_id="NP_998764.1"
                     /db_xref="CCDS:CCDS31444.1"
                     /db_xref="GeneID:203859"
                     /db_xref="HGNC:HGNC:27337"
                     /db_xref="MIM:608662"
                     /translation="MGDPDLLEVLAEEGEKVNKHIDYSFQMSEQSLSSRETSFLINEE
                     TMPAKRFNLFLRRRLMFQKNQQSKDSIFFRDGIRQIDFVLSYVDDVKKDAELKAERRK
                     EFETNLRKTGLELEIEDKRDSEDGRTYFVKIHAPWEVLVTYAEVLGIKMPIKESDIPR
                     PKHTPISYVLGPVRLPLSVKYPHPEYFTAQFSRHRQELFLIEDQATFFPSSSRNRIVY
                     YILSRCPFGIEDGKKRFGIERLLNSNTYSSAYPLHDGQYWKPSEPPNPTNERYTLHQN
                     WARFSYFYKEQPLDLIKNYYGEKIGIYFVFLGFYTEMLFFAAVVGLACFIYGLLSMEH
                     NTSSTEICDPEIGGQMIMCPLCDQVCDYWRLNSTCLASKFSHLFDNESTVFFAIFMGI
                     WVTLFLEFWKQRQARLEYEWDLVDFEEEQQQLQLRPEFEAMCKHRKLNAVTKEMEPYM
                     PLYTRIPWYFLSGATVTLWMSLVVTSMVAVIVYRLSVFATFASFMESDASLKQVKSFL
                     TPQITTSLTGSCLNFIVILILNFFYEKISAWITKMEIPRTYQEYESSLTLKMFLFQFV
                     NFYSSCFYVAFFKGKFVGYPGKYTYLFNEWRSEECDPGGCLIELTTQLTIIMTGKQIF
                     GNIKEAIYPLALNWWRRRKARTNSEKLYSRWEQDHDLESFGPLGLFYEYLETVTQFGF
                     VTLFVASFPLAPLLALINNIVEIRVDAWKLTTQYRRTVASKAHSIGVWQDILYGMAVL
                     SVATNAFIVAFTSDIIPRLVYYYAYSTNATQPMTGYVNNSLSVFLIADFPNHTAPSEK
                     RDFITCRYRDYRYPPDDENKYFHNMQFWHVLAAKMTFIIVMEHVVFLVKFLLAWMIPD
                     VPKDVVERIKREKLMTIKILHDFELNKLKENLGINSNEFAKHVMIEENKAQLAKSTL"
     exon            449..495
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /inference="alignment:Splign:2.1.0"
     exon            496..546
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /inference="alignment:Splign:2.1.0"
     exon            547..588
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /inference="alignment:Splign:2.1.0"
     exon            589..702
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /inference="alignment:Splign:2.1.0"
     exon            703..771
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /inference="alignment:Splign:2.1.0"
     exon            772..1056
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1170
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..1286
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /inference="alignment:Splign:2.1.0"
     exon            1287..1421
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1527
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /inference="alignment:Splign:2.1.0"
     exon            1528..1588
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1740
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /inference="alignment:Splign:2.1.0"
     exon            1741..1815
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /inference="alignment:Splign:2.1.0"
     exon            1816..2038
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /inference="alignment:Splign:2.1.0"
     exon            2039..2208
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /inference="alignment:Splign:2.1.0"
     exon            2209..2306
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /inference="alignment:Splign:2.1.0"
     exon            2307..2437
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /inference="alignment:Splign:2.1.0"
     exon            2438..2643
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /inference="alignment:Splign:2.1.0"
     exon            2644..2822
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /inference="alignment:Splign:2.1.0"
     exon            2823..2928
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /inference="alignment:Splign:2.1.0"
     exon            2929..6742
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6098..6103
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /note="hexamer: AATAAA"
     polyA_site      6117
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /note="major polyA site"
     regulatory      6718..6723
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /note="hexamer: AATAAA"
     polyA_site      6742
                     /gene="ANO5"
                     /gene_synonym="GDD1; LGMD2L; LGMDR12; TMEM16E"
                     /note="major polyA site"
ORIGIN      
        1 ggaaaggatc ctggcgagca ccgggaggag tggcggctgc gggatcagct gccgagcagg
       61 cacagggaca ggtgcctgga gaagtactgg gagagcgccc aggagcgcta ccggctgagg
      121 gtggggaagc gcagggccaa gcgcgcgaag caggttgtgg gggaccgggt cgagtggaag
      181 tacccgccgg agaggaaggc cggctggctg tggcgcccag agacggtgga gtccgaggag
      241 gaggagaagg aggcctgcag aaggaagagc aggcccttag aagtccagca gcagcaactc
      301 cggcggccca cagtcagatt cagcacctgc ctcagatctc cacgtctgtc tcagctgccc
      361 ctctcctgct gcctctcagg caccagtgcc attaacgagc tggcgaagat gggcgacccg
      421 gatctcctgg aagtgttggc ggaggaaggg gaaaaagtca ataagcatat agactactct
      481 ttccaaatga gtgagcagag cctgagcagc agagagacca gctttctcat caatgaagaa
      541 acaatgcctg caaagcgatt caatttgttc ctgaggcggc ggcttatgtt tcaaaaaaat
      601 cagcaaagca aagattctat cttcttccga gatgggatta ggcaaattga ttttgtgctt
      661 tcctacgttg atgatgtaaa gaaagacgca gagttaaagg cggaaagaag aaaagagttt
      721 gaaactaatc tcagaaaaac aggtcttgag ttggaaatag aagacaaaag ggactcggaa
      781 gatggaagaa cttattttgt caagatccat gccccttggg aggtattagt tacctatgct
      841 gaagtcttgg gaatcaaaat gcctattaag gagagtgata ttccccgccc taagcacact
      901 cctataagct atgtgcttgg acctgtaaga ctcccactga gtgtgaagta tccccatcct
      961 gaatatttta ctgcacaatt cagcagacat cggcaggagc tcttcctcat cgaagatcag
     1021 gcaaccttct ttccatcctc atcaagaaac agaattgtgt actatattct ctcaagatgt
     1081 ccttttggca tagaagatgg gaagaaaagg tttgggattg aaagactgct aaactctaac
     1141 acttactcat ctgcctatcc actccatgat ggccaatatt ggaagccatc agaacctccc
     1201 aatcctacca atgaaagata cacacttcac cagaattggg ctcgattttc ctatttctac
     1261 aaggagcagc ctttagactt gattaagaat tattatggag aaaaaattgg tatctatttt
     1321 gtctttcttg gattttacac agaaatgcta ttctttgcag ctgtagttgg cttagcttgt
     1381 tttatttatg gcttattatc aatggaacat aacacaagca gcactgaaat ctgtgaccct
     1441 gagattggtg gtcagatgat catgtgccca ctctgtgatc aagtgtgtga ttattggaga
     1501 ctaaatagta cgtgtttggc ttcaaagttc tcccatttgt ttgataatga gtcaacagtg
     1561 ttctttgcaa tattcatggg aatttgggtc accttatttt tggagttttg gaaacaacga
     1621 caagccagac tggaatatga atgggacctg gtggactttg aagaggaaca gcagcagctt
     1681 cagctgagac cagaatttga agctatgtgt aaacacagga aattgaatgc agtgactaag
     1741 gagatggaac cttacatgcc tctatacacg cgtattccat ggtactttct ttcaggagcc
     1801 acagtgacat tatggatgtc tcttgtcgtc accagtatgg tagctgtaat tgtgtaccgc
     1861 ctgtcagtct ttgctacatt tgctagtttc atggaaagtg atgcatcctt aaagcaggtc
     1921 aaaagcttcc ttactcctca gataaccaca tcactcacag gatcatgctt gaactttatt
     1981 gtcatcttga tcttgaattt cttttatgaa aagatatctg cctggatcac aaaaatggaa
     2041 attcctcgaa cataccagga gtatgagagc agtcttacct tgaaaatgtt cctgtttcag
     2101 tttgtaaatt tttactcatc ctgcttctac gtagctttct ttaaagggaa gttcgtaggc
     2161 tatcctggaa aatacacata tttatttaat gagtggagaa gtgaagagtg tgatcctgga
     2221 ggctgtctta tagaattgac aacccaattg accattataa tgaccgggaa acagattttt
     2281 ggaaacatta aagaagccat ttatcccttg gctttgaatt ggtggagacg ccgaaaagct
     2341 cggacaaact ctgagaagct gtatagtcga tgggagcagg atcatgacct tgaaagtttt
     2401 ggaccccttg ggcttttcta tgagtactta gaaacagtta ctcaatttgg atttgttaca
     2461 ctatttgtgg cctcttttcc tttggctcct cttcttgctc tcataaataa tattgtagag
     2521 attcgagtgg atgcctggaa acttaccact caatacagga gaactgtagc ttctaaagct
     2581 catagcatag gtgtttggca agacattctt tatggaatgg ctgtcctttc tgttgcaact
     2641 aatgccttta ttgttgcatt tacgtcagac atcattcccc gtctagttta ctactatgct
     2701 tactcaacaa atgccacaca gcctatgaca ggatatgtga ataatagcct gtcagtattc
     2761 ctgatagctg attttccaaa ccacactgca ccttcggaaa aacgagactt catcacttgc
     2821 aggtacagag attacagata tcctcctgat gacgagaata aatattttca taatatgcaa
     2881 ttctggcatg tccttgctgc caagatgacc ttcatcattg ttatggaaca tgttgtgttt
     2941 ttagttaaat ttttgctggc ctggatgata cctgatgttc caaaagatgt tgtggagaga
     3001 atcaagagag aaaagttaat gactatcaag attctccatg attttgagct caacaaatta
     3061 aaagagaact tgggaattaa ttctaatgaa tttgccaagc atgtcatgat tgaggaaaac
     3121 aaagcacagc tggctaaatc aacactctaa tcagtatagt gaggaagcag caggtgatct
     3181 gccttacttc actttatcct ctggttttag ggccagacgc cagaagccat gtgtcaattt
     3241 taccctttct tttttttttt tttctttttt tttttaaact caaagttttt atacactttt
     3301 atagaggcca actttgtgat gttggaaatg tactacttct ctgcttcatt gactgggccc
     3361 tctccagatg ttgttttctg aggtgctgta aatgactgtt gaaagtgcag gtagaatcag
     3421 aatactggga aattatggag tcttgcagtt tagtaagaaa cactggcctt gggctgtccc
     3481 atcactttcc agtgcatcta tttatttttg tggtcttctc ttgggttatt tgatacctcc
     3541 ttccccatta agaaaaatgt tggggcaaaa agaaatggat caaagagact gactgagccc
     3601 tatatatcct atcattttaa aatatgcaaa tgaattgcca agatcagatg acataagaaa
     3661 actcacacat taaggtgtta atgtatcata gcagaggttt attcctaaca cattcaacta
     3721 ccatcagaat cccagatagt tcttcctggt aaaggcagaa ttccttttct cgagactgaa
     3781 attttgggtt tcaacataaa acaacttggt tcttagagat aataatttgg ttataatagt
     3841 ttcaagactg atcttatctg gaaagcaaca ttatgaagct gttagattgc ttcaggttct
     3901 caagcaaaga cacaatacag aagtaaatgt gttttcttag tagttaatgg atgcaggaca
     3961 atgtatattg attaatttgt tgattttaat ttagaaaatt gttaaattat ttcttaaaaa
     4021 tcacttttct tctggaatgc caatttcaca tcatgaagcc tttttgtata agttagatac
     4081 gagttgttta tgataaacat ttctttgctt taaaataatt gcaaatattt taataagttt
     4141 acaacctttt ctattgatgt atcatcttat acaatgctca gtgccttgtt ccaatacctc
     4201 tgacacacaa gaagtcatgt tgttagctag tgatttgatg tgatgtaaca tcttaaatgt
     4261 aagcttgtct taatgaaatt gtcagtgtaa taacaactac agtcttgaaa accaaaagtg
     4321 aatcaaccaa ctaagaatga gttcatggac ttaataatct aagggggaaa aaatgtttgt
     4381 tgaattattc ctctcaaatt taggcttgtg ttacatgcac aaaaatcctt gttctgtttt
     4441 cacttaaaaa aactaaatat gtataacttt gtgtatacac acacacacat ctatatatat
     4501 aattattagc actagaggga tatagtccag ttatgtagta tttaaatctc cagtttcaaa
     4561 ttataattca cctccaaaag aatagttttt taatcacaca cataagaaat tttatcacaa
     4621 tatttaaaac taatatttca ttatctaatg ctaataaatt attgtggtac tgccagtatt
     4681 aaatatatgg cagatggtat taactactga tcaatagtaa gcatacagaa ctggggatta
     4741 tggattttat aaactatgag acagtcaccc cagtttggac tgggactaat ccccagtact
     4801 gatttgtcat ccactgagta gactttatga atattttggg taatttgaaa tgatctcatt
     4861 attgaaagat gatttcatat gtagagaaga taatatttct ttcttgaaaa aacaagtcag
     4921 gcttaccatg atgtgtgcaa ccaatgtagg atctttggct tgtcaaatca gattctccat
     4981 tgctatagtg tacagtgcac acagctcata ttgcttcctt cctgggtgct gataaaaata
     5041 agaaagagca tggaaattgg tttcttgaat ataagcttta atttttaagg cttaaaagta
     5101 ttcatagagg tagactgtat gataaataaa aacaaattta attcacaaag ttatctgtac
     5161 actgcagttt taaaatatac caactaaatt attgggtttc tggaagtgaa tggagaaaac
     5221 agcaagggaa gaaatcgttt ttaagataag taaataattc ccatggattg ataaatattt
     5281 tccttttaaa atgttatgga ctgatatttt ttattcacct ttaaatttct tatcaagaag
     5341 tttatctttg tttttcagat ttaaaaatga aatacaggta ttcgtcactt tcctgaaacc
     5401 atgctaacca aaatcagtag ccaaaccaat tcagatagat gtgtctcatc taattaaacc
     5461 cattggtttt tatgggaggg ctgcattaag agcacccaac caccacatgt aagttgataa
     5521 ttaccagcat ggcaggtgat tttatctgct gaccaagcgc atagttttgt tttgttttca
     5581 gaatgttcta gggaacattt gagattttat gtgaaataaa attttaagtg ccaaagccaa
     5641 aaaaatactt aactcttttc aaaggccctc tttcatcctt gccttcgtca actttccttt
     5701 gcacacagga agcaaaatct acttcaagac atttatttta gaggaatcca ttaagaaagg
     5761 attgttttac ttaaatgaaa tgctgtctta tttttgctgt gtcttttgac acctcttagc
     5821 tttaacattc ttcttttaac caagccatta tgcaaagtta tcaaagaaga ggagaaggaa
     5881 aggaggacag atgaaagtga cgggaaatga gaaagaagag gagtgaggaa acagttattt
     5941 ccttatgaat cctgtgtgtt tctttggaag aaagagctgt tccgaggaag tttggtccag
     6001 ctgtggttga taagaggtat atctcttaaa aaagacacct aatgaaagtg agagaaaagc
     6061 taaagaaaat ttcaatgtga ccactattat gtgtcaaaat aaaacttaga ttccaaagtg
     6121 gttttgtagt gttgggtgct atgagtaggt atggatctct gttgattgac tccagttgaa
     6181 ggtgagaaat ctgtaccaat cattcaaaaa gggaatctat tgttttgaaa gaaactctct
     6241 catatttcct ttaaattgtt aatagttgtt atgcaactaa agaatgttat gaagaaaaat
     6301 agcattgcaa aaagtaccat tggccagcct tacaagtcag ccacaatgag tcggtataac
     6361 tactgtgtta ttctttttct agacaaattt tgactcttct acttttatgt gtaataattc
     6421 cagtattcta tttatttcag cattatgtga aaaatgataa gaatgttaaa aagaaaataa
     6481 tagtgtggtt taattggtat gagatacctg cttcctcctc ctcaaaggtt tgactgggtg
     6541 tatctctcct atgtgtgaca ttatgtctcc tggtgttaac acaggaaatg agtgctcctt
     6601 tttaaaattt ctttcttcca agtttttttt tccaggaaga gaaatatgca gttatgcagg
     6661 aaaagctctc ttaaataatg tgtacataaa tctcaagaga atcaaattca cagagtgaat
     6721 aaagtaataa tattaaacta ca
//
