LOCUS       NM_031418               5937 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens anoctamin 3 (ANO3), transcript variant 2, mRNA.
ACCESSION   NM_031418
VERSION     NM_031418.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Ousingsawat,J., Talbi,K., Gomez-Martin,H., Koy,A.,
            Fernandez-Jaen,A., Tekgul,H., Serdaroglu,E., Schreiber,R.,
            Ortigoza-Escobar,J.D. and Kunzelmann,K.
  TITLE     Broadening the clinical spectrum: molecular mechanisms and new
            phenotypes of ANO3-dystonia
  JOURNAL   Brain 147 (6), 1982-1995 (2024)
   PUBMED   38079528
  REMARK    GeneRIF: Broadening the clinical spectrum: molecular mechanisms and
            new phenotypes of ANO3-dystonia.
REFERENCE   2  (bases 1 to 5937)
  AUTHORS   Percetti,M., Zini,M., Soliveri,P., Cogiamanian,F., Ferrara,M.,
            Orunesu,E., Ranghetti,A., Ferrarese,C., Pezzoli,G., Garavaglia,B.,
            Isaias,I.U. and Sacilotto,G.
  TITLE     The Clinical Spectrum of ANO3-Report of a New Family and Literature
            Review
  JOURNAL   Mov Disord Clin Pract 11 (3), 289-297 (2024)
   PUBMED   38284143
  REMARK    GeneRIF: The Clinical Spectrum of ANO3-Report of a New Family and
            Literature Review.
            Review article
REFERENCE   3  (bases 1 to 5937)
  AUTHORS   Esposito,M., Trinchillo,A., Piceci-Sparascio,F., D'Asdia,M.C.,
            Consoli,F. and De Luca,A.
  TITLE     A novel ANO3 variant in two siblings with different phenotypes
  JOURNAL   Parkinsonism Relat Disord 111, 105413 (2023)
   PUBMED   37116293
  REMARK    GeneRIF: A novel ANO3 variant in two siblings with different
            phenotypes.
REFERENCE   4  (bases 1 to 5937)
  AUTHORS   Jiang,L.T., Li,L.X., Liu,Y., Zhang,X.L., Pan,Y.G., Wang,L.,
            Wan,X.H. and Jin,L.J.
  TITLE     The expanding clinical and genetic spectrum of ANO3 dystonia
  JOURNAL   Neurosci Lett 746, 135590 (2021)
   PUBMED   33388357
  REMARK    GeneRIF: The expanding clinical and genetic spectrum of ANO3
            dystonia.
REFERENCE   5  (bases 1 to 5937)
  AUTHORS   Ma,L.Y., Wang,L., Yang,Y.M., Feng,T. and Wan,X.H.
  TITLE     Mutations in ANO3 and GNAL gene in thirty-three isolated dystonia
            families
  JOURNAL   Mov Disord 30 (5), 743-744 (2015)
   PUBMED   25847575
  REMARK    GeneRIF: This study demonstrated that Mutations in ANO3 may cause
            Dystonia.
REFERENCE   6  (bases 1 to 5937)
  AUTHORS   Huang,F., Wang,X., Ostertag,E.M., Nuwal,T., Huang,B., Jan,Y.N.,
            Basbaum,A.I. and Jan,L.Y.
  TITLE     TMEM16C facilitates Na(+)-activated K+ currents in rat sensory
            neurons and regulates pain processing
  JOURNAL   Nat Neurosci 16 (9), 1284-1290 (2013)
   PUBMED   23872594
  REMARK    GeneRIF: rat Ano3 (also known as Tmem16c) interacts with, and
            alters the activity of the sodium-activated potassium channel
            Slack. Reduced expression of Ano3 in rat models results in
            increased pain sensitivity.
REFERENCE   7  (bases 1 to 5937)
  AUTHORS   Charlesworth,G., Plagnol,V., Holmstrom,K.M., Bras,J., Sheerin,U.M.,
            Preza,E., Rubio-Agusti,I., Ryten,M., Schneider,S.A., Stamelou,M.,
            Trabzuni,D., Abramov,A.Y., Bhatia,K.P. and Wood,N.W.
  TITLE     Mutations in ANO3 cause dominant craniocervical dystonia: ion
            channel implicated in pathogenesis
  JOURNAL   Am J Hum Genet 91 (6), 1041-1050 (2012)
   PUBMED   23200863
  REMARK    GeneRIF: Mutations in ANO3 are a cause of autosomal-dominant
            craniocervical dystonia.
REFERENCE   8  (bases 1 to 5937)
  AUTHORS   Hartzell,H.C., Yu,K., Xiao,Q., Chien,L.T. and Qu,Z.
  TITLE     Anoctamin/TMEM16 family members are Ca2+-activated Cl- channels
  JOURNAL   J Physiol 587 (Pt 10), 2127-2139 (2009)
   PUBMED   19015192
  REMARK    Review article
REFERENCE   9  (bases 1 to 5937)
  AUTHORS   Katoh,M. and Katoh,M.
  TITLE     Identification and characterization of TMEM16E and TMEM16F genes in
            silico
  JOURNAL   Int J Oncol 24 (5), 1345-1349 (2004)
   PUBMED   15067359
REFERENCE   10 (bases 1 to 5937)
  AUTHORS   Katoh,M. and Katoh,M.
  TITLE     FLJ10261 gene, located within the CCND1-EMS1 locus on human
            chromosome 11q13, encodes the eight-transmembrane protein
            homologous to C12orf3, C11orf25 and FLJ34272 gene products
  JOURNAL   Int J Oncol 22 (6), 1375-1381 (2003)
   PUBMED   12739008
  REMARK    GeneRIF: C11orf25, FLJ10261 (ORAOV2), C12orf3 and FLJ34272
            constitute a family of eight-transmembrane proteins with N- and
            C-terminal tails facing the cytoplasm.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC021698.12, AJ300461.1,
            DA247850.1, AC079064.6, BQ430769.1, AC083755.10 and X81896.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_031418.3.
            
            Summary: The protein encoded by this gene belongs to the TMEM16
            family of predicted membrane proteins, that are also known as
            anoctamins. While little is known about the function of this gene,
            mutations in this gene have been associated with some cases of
            autosomal dominant craniocervical dystonia. Cells from individuals
            with a mutation in this gene exhibited abnormalities in endoplasmic
            reticulum-dependent calcium signaling. Studies in rat show that the
            rat ortholog of this protein interacts with, and modulates the
            activity of a sodium-activated potassium channel. Deletion of this
            gene caused increased pain sensitivity in the rat model system.
            Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Aug 2015].
            
            Transcript Variant: This variant (2) differs in the 5' UTR, lacks a
            portion of the 5' coding region, contains an alternate 5' terminal
            exon, and initiates translation at an alternate start codon,
            compared to variant 1. The encoded isoform (2) has a distinct
            N-terminus and is shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.53332.1,
                                           SRR1803611.60310.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2145743
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000256737.8/ ENSP00000256737.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 AC021698.12        92733-92733         c
            2-630               AJ300461.1         708-1336
            631-640             DA247850.1         590-599
            641-672             AJ300461.1         1347-1378
            673-673             AC079064.6         115969-115969
            674-911             AJ300461.1         1380-1617
            912-912             AC079064.6         139004-139004
            913-1303            AJ300461.1         1619-2009
            1304-1304           AC079064.6         155190-155190
            1305-2134           AJ300461.1         2011-2840
            2135-2821           BQ430769.1         5-691
            2822-2827           AJ300461.1         3528-3533
            2828-2829           BQ430769.1         698-699
            2830-3708           AJ300461.1         3536-4414
            3709-5063           AC083755.10        120578-121932       c
            5064-5937           X81896.1           334-1207
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p14.3-p14.2"
     gene            1..5937
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /note="anoctamin 3"
                     /db_xref="GeneID:63982"
                     /db_xref="HGNC:HGNC:14004"
                     /db_xref="MIM:610110"
     exon            1..192
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     CDS             147..3092
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /note="isoform 2 is encoded by transcript variant 2;
                     transmembrane protein 16C (eight membrane-spanning
                     domains); dystonia 23"
                     /codon_start=1
                     /product="anoctamin-3 isoform 2"
                     /protein_id="NP_113606.2"
                     /db_xref="CCDS:CCDS31447.1"
                     /db_xref="GeneID:63982"
                     /db_xref="HGNC:HGNC:14004"
                     /db_xref="MIM:610110"
                     /translation="MVHHSGSIQSFKQQKGMNISKSEITKETSLKPSRRSLPCLAQSY
                     AYSKSLSQSTSLFQSTESESQAPTSITLISTDKAEQVNTEENKNDSVLRCSFADLSDF
                     CLALGKDKDYTDESEHATYDRSRLINDFVIKDKSEFKTKLSKNDMNYIASSGPLFKDG
                     KKRIDYILVYRKTNIQYDKRNTFEKNLRAEGLMLEKEPAIASPDIMFIKIHIPWDTLC
                     KYAERLNIRMPFRKKCYYTDGRSKSMGRMQTYFRRIKNWMAQNPMVLDKSAFPDLEES
                     DCYTGPFSRARIHHFIINNKDTFFSNATRSRIVYHMLERTKYENGISKVGIRKLINNG
                     SYIAAFPPHEGAYKSSQPIKTHGPQNNRHLLYERWARWGMWYKHQPLDLIRLYFGEKI
                     GLYFAWLGWYTGMLIPAAIVGLCVFFYGLFTMNNSQVSQEICKATEVFMCPLCDKNCS
                     LQRLNDSCIYAKVTYLFDNGGTVFFAIFMAIWATVFLEFWKRRRSILTYTWDLIEWEE
                     EEETLRPQFEAKYYKMEIVNPITGKPEPHQPSSDKVTRLLVSVSGIFFMISLVITAVF
                     GVVVYRLVVMEQFASFKWNFIKQYWQFATSAAAVCINFIIIMLLNLAYEKIAYLLTNL
                     EYPRTESEWENSFALKMFLFQFVNLNSSIFYIAFFLGRFVGHPGKYNKLFDRWRLEEC
                     HPSGCLIDLCLQMGVIMFLKQIWNNFMELGYPLIQNWWSRHKIKRGIHDASIPQWEND
                     WNLQPMNLHGLMDEYLEMVLQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFVTQW
                     RRPLPARATDIGIWLGILEGIGILAVITNAFVIAITSDYIPRFVYEYKYGPCANHVEP
                     SENCLKGYVNNSLSFFDLSELGMGKSGYCRYRDYRGPPWSSKPYEFTLQYWHILAARL
                     AFIIVFEHLVFGIKSFIAYLIPDVPKGLHDRIRREKYLVQEMMYEAELEHLQQQRRKS
                     GQPVHHEWP"
     exon            193..387
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            388..459
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            460..578
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            579..737
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            738..838
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            839..883
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            884..1015
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1122
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            1123..1178
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            1179..1300
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..1435
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            1436..1532
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            1533..1593
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            1594..1676
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            1677..1817
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            1818..1982
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            1983..2019
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            2020..2131
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            2132..2189
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            2190..2287
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            2288..2421
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            2422..2574
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            2575..2722
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            2723..2803
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            2804..2909
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     exon            2910..5937
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5914..5919
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /note="hexamer: AATAAA"
     polyA_site      5937
                     /gene="ANO3"
                     /gene_synonym="C11orf25; DYT23; DYT24; GENX-3947; TMEM16C"
                     /note="major polyA site"
ORIGIN      
        1 ggagagcgaa gtgccggcta cagcaggtgt cggattgcag tgcgctcgct gaggctccgg
       61 accttggagc gtctagagtc tggctactgt tcctccgcct ccctctcggg cagctcccta
      121 agccggctgg gacgcgcaga gtgaaaatgg tccaccattc aggctccatt cagtccttta
      181 aacagcaaaa aggtatgaat ataagcaaga gtgagataac aaaagaaact tcgttaaaac
      241 cgtctcggag atccctgcct tgcctcgccc agagctacgc ttactcaaag agcttgagcc
      301 agtctacttc cctcttccag tcaaccgaga gtgaatctca ggctcccaca tctataacct
      361 taatctccac tgacaaagca gagcaagtta atactgagga gaataaaaac gactctgtgc
      421 tgagatgttc atttgctgac ctcagcgatt tttgtttggc cctaggaaaa gataaggatt
      481 acacggatga atcagaacac gctacttatg accgatctcg tctcattaat gactttgtta
      541 tcaaagataa atctgaattc aagacaaaat tatctaagaa tgacatgaat tacatagcat
      601 ccagtggacc tctgttcaaa gatggcaaaa agagaattga ttacatcttg gtttatagaa
      661 agacaaatat acaatatgat aaaagaaaca catttgaaaa gaacctcaga gcagaaggct
      721 tgatgttgga gaaggagcca gctattgcaa gccccgatat catgtttatt aaaattcaca
      781 ttccatggga cacgctgtgc aagtatgcag agaggctgaa tatcaggatg cccttcagga
      841 aaaaatgcta ttacactgac gggaggagca aatcaatggg caggatgcaa acttatttta
      901 gaagaatcaa aaactggatg gcccaaaacc caatggttct tgacaagtca gcttttccag
      961 acctagagga gtcagactgc tatactggcc ccttcagccg tgcacggatt caccacttca
     1021 taataaataa taaagacacc ttcttcagca atgctactcg aagcagaata gtctatcaca
     1081 tgctggaacg caccaaatat gaaaatggaa tatcaaaagt gggtatccgt aaacttataa
     1141 acaatggctc atacatagca gcgtttccac cacatgaggg agcctacaaa agtagccagc
     1201 ccattaaaac ccatggacct cagaataaca gacatctatt atatgagcgc tgggcacgct
     1261 ggggaatgtg gtataagcat cagcctctgg atttaatcag gctatacttt ggtgagaaga
     1321 ttggactata ctttgcttgg ctgggatggt atactggaat gttgattcct gcagcaattg
     1381 ttggtttgtg cgttttcttc tatggattat ttacaatgaa taatagtcaa gtaagccaag
     1441 aaatttgtaa agccactgaa gtctttatgt gccctctctg tgacaagaac tgctccctgc
     1501 agagactcaa cgacagctgt atctatgcca aggtgacata tttgttcgat aatggaggga
     1561 cagtcttctt tgctattttt atggcaatat gggccacagt cttcctggag ttttggaaaa
     1621 ggagaaggag tatactgacc tatacttggg accttatcga atgggaagaa gaggaggaaa
     1681 cacttcgtcc ccagtttgaa gccaagtatt acaagatgga gattgtaaat cccatcacgg
     1741 gaaaacctga accacatcag ccttcctcag acaaagtcac tcgtcttctt gtttctgtct
     1801 caggaatatt cttcatgata tccttggtga tcactgcagt gtttggagtt gtggtgtacc
     1861 gcctggttgt catggaacag tttgcatcat tcaagtggaa tttcatcaaa caatactggc
     1921 agtttgcaac atctgctgct gctgtctgta tcaatttcat aatcattatg ttgctgaatc
     1981 ttgcttatga aaaaattgct tacctcctca ccaatttaga atatcctcga acagaatcag
     2041 agtgggaaaa cagcttcgcc ctgaagatgt tcctcttcca gtttgtcaat ttaaacagtt
     2101 ccatcttcta tatcgctttc tttttgggaa gattcgtagg ccacccagga aaatacaata
     2161 aactttttga ccggtggaga ctggaggaat gtcatcctag tggctgtttg atagacctct
     2221 gcctccagat gggtgtcatc atgtttttga agcaaatatg gaacaacttc atggaactag
     2281 gatacccgtt gatccagaac tggtggtcac gacataaaat caagcgggga atacatgatg
     2341 cttccatacc tcagtgggaa aatgattgga atctgcagcc catgaacctt catggactga
     2401 tggatgagta cttagaaatg gttttgcaat ttggttttac caccatcttt gttgcggctt
     2461 ttcctctagc ccctcttttg gctttgttaa acaatatcat tgaaatcagg ctggatgcat
     2521 acaaatttgt cactcaatgg cggaggcctt tgccagcccg agcaactgac ataggtatct
     2581 ggcttggaat tctcgaagga atcggtatat tggctgtgat caccaatgca tttgtaattg
     2641 ctattacttc tgattacatc ccacgttttg tttatgaata caaatatggc ccctgtgcaa
     2701 atcatgtaga accaagtgaa aattgcttga agggatatgt caacaatagc ctatccttct
     2761 ttgacctgag tgagcttggt atgggaaaat ctggttattg caggtacaga gactacagag
     2821 gcccgccctg gagttccaaa ccctatgagt ttactttaca atactggcat atccttgctg
     2881 ctagattggc cttcattatt gtgtttgagc accttgtttt tgggattaag tcattcatcg
     2941 catacctgat tccagacgta ccaaagggtc tacatgaccg aatacgacga gagaagtact
     3001 tagttcaaga aatgatgtat gaggctgaac tggaacattt gcaacaacaa cggagaaaaa
     3061 gtggtcagcc tgttcaccat gaatggcctt agttgacacc tgttacccat taggggtgat
     3121 aacattaatg ggaagaaatg atggcaactt tgaatgctag gtgaaatcta ggaggaaggc
     3181 atacttggca aaccacatgt ataatatgct acttggaaat gtatcacagc catctctggg
     3241 atttgaaata tccagacttg tagggaagaa aacaatgact tgacgacctt aaaaagggtt
     3301 agattgacat tgcaggaagc caggatgtaa ttctcagaac cagtctcagg gagatatatg
     3361 cttggagaac tctgccttcc atcaactgca gtgtaatggg aaagagggtg gtgaggtttt
     3421 gaaagacaga tttccatgta aatgtgcaca agccaggcat ggcttaaaat atcatgcagt
     3481 cttcactcac atactaattt gactttggaa agtatggttg agttcagata cttataagaa
     3541 atgacttaaa caaatccttg ccttggctgc cagaactcta catcccttca gtttacaggt
     3601 tggtaggaat tgcccatgtc tcttgctgag attatggatc tgtgccacag gggatttatg
     3661 ctgctttaca ttgactatgc agttgaagag ttgcacatca cctttagtag tcaaaccaaa
     3721 aatctaagat tccaaaaaga acagcattca ttgaaataat attccattgc attggctact
     3781 tcaagtctct tttacccctg gcagagccag ttgcagtgca attttttgta ctaaatgcca
     3841 ataaagccac atacttataa actatgaatg gctattctta tatagtttag aacacaacta
     3901 atccatactt tattgtggaa ggctgtttta tagtttattt aattttttct ctatgattta
     3961 ttacaactct cacggaatat taacttgaaa tgctgtaaat acgttggttt tccttgtccc
     4021 tttgcacctt gacagtacgt agtatacatt gtggtttatg cagctctgac accagttttt
     4081 gctattgtaa tatgtttatt cgggaaaact gaggccagat gctctgcaac acttacgctt
     4141 tttcaacaat gtgcactctt actcatgaac taatgaaaat aatgcatgaa tataaatcta
     4201 tattatttgc taaaaaaaga aaaaacattt gtgttttagt ttcttttttc atgactgaag
     4261 ggagttttat gttattattt cttccatagt tttgttttgg tttattttta acaacgctct
     4321 agaaacaaag tcaaattaat cacaaaagac ataattttgc tttgttgtgg aatttctatt
     4381 tcaacgtcaa ctctgtatct atgagtatgt ctgttccaca gacagatgag gcaggagtga
     4441 tggggcactc aagaaagttc agaggaggca taagctatgg aggttggaaa ggaagaaaga
     4501 gaagagctga agtaaatgta tggtagaaat taagacgttt ctttgcaaaa caaggaaatc
     4561 acacatatgc acacatacat atttatggtt actatgattg ttattatttg tccaatcaag
     4621 caaagcacca ttatttctaa catataaaag accagatcaa acacaatgga aataaagcta
     4681 ctacatatat atgccagtat ttctctagta agattatgtt tctcttacta gaaaactatt
     4741 ttctaaatat taacactgaa aatgttttgt tagcttttcc ttctttctct ccagaagaaa
     4801 catggataga tgatagctgt ttcattgttt gtttttgtca agcatattca ctttcctcct
     4861 tgtcctctga ttctgagcaa agggcctcag actctgaact tccctcaagt gccgttgtta
     4921 tgtgaaactc ttccattcag attccagaga ggttctcatg ctcccccccc tccttatttg
     4981 tagcaatcgt agcaactaat tccactaagt acaagggagt tttttacact cctccatttt
     5041 tatagcatct gcatttcttt ttctttgtta ggtacatgta tacacctgcc tgagtataaa
     5101 tactctctct acctaataat aacatcaacc aacatctttt ccaaattagg gccacagaac
     5161 agcaacattt gtctgacagt agtataaaga ataatgatag ctctatcctt aagaagtatt
     5221 tcctttcctt tttatatagt cccgttaggg tttaaaacca tattgatcaa ctagaaagaa
     5281 aaatatgaaa agagaaaaat attttaattt aaaaattgta atacattgat ttataaaatg
     5341 ccttctctga tacttttgaa acagatgtga aaaacagaaa aagaaaaaat tgtctgaaat
     5401 gtttattttg caaaacagtg caatagaatc tagttatgcc ttcatcactg ttgacagtaa
     5461 atactgacag ccccttgcag tgtgttagtt ttagatcact ctgttttagt tgagagaaat
     5521 gttttatatc atggttttta tatgaataca aattatttct caaagattta tagcacacac
     5581 tattctcagg aattctgtat tacatgaatg ctgcttatat attttcatat tctaacttgt
     5641 cttttcaagc aaataactaa tatatatgtg catgcagtct gccttgacaa gttgttccaa
     5701 gctgaagagc tttcactgta caatgtgtgg aaaatcacca tagatcatgg ctgaaatagt
     5761 ttgtaattgt ctgagtctgt gcacgtactt ttagataaaa tgctgctgag tgactgcatg
     5821 atgagataca acttctgaat gctgcacatt cttccaaaat gatccttagc acaatctatt
     5881 gtatgatgga atgaatagaa aactttttca ctcaataaat tattatttga tatggta
//
