LOCUS       NM_018043               4777 bp    mRNA    linear   PRI 19-AUG-2024
DEFINITION  Homo sapiens anoctamin 1 (ANO1), transcript variant 1, mRNA.
ACCESSION   NM_018043
VERSION     NM_018043.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Yang,X., Cui,L., Liu,Z., Li,Y., Wu,X., Tian,R., Jia,C., Ren,C.,
            Mou,Y. and Song,X.
  TITLE     TMEM16A inhibits autophagy and promotes the invasion of
            hypopharyngeal squamous cell carcinoma through mTOR pathway
  JOURNAL   Carcinogenesis 45 (8), 569-581 (2024)
   PUBMED   38470063
  REMARK    GeneRIF: TMEM16A inhibits autophagy and promotes the invasion of
            hypopharyngeal squamous cell carcinoma through mTOR pathway.
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Shin,Y., Kim,S. and An,W.
  TITLE     Promoter hypermethylation as a novel regulator of ANO1 expression
            and function in prostate cancer bone metastasis
  JOURNAL   Sci Rep 14 (1), 11595 (2024)
   PUBMED   38773164
  REMARK    GeneRIF: Promoter hypermethylation as a novel regulator of ANO1
            expression and function in prostate cancer bone metastasis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4777)
  AUTHORS   Liu,X.M., Li,J., Chen,D., Li,H., Qin,X.Y., Wang,Y.X., Gu,Y.Z.,
            Li,N., Zhou,L.G. and Feng,M.
  TITLE     Ano1 regulates embryo transport by modulating intracellular calcium
            levels in oviduct smooth muscle
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (4), 167059 (2024)
   PUBMED   38336104
  REMARK    GeneRIF: Ano1 regulates embryo transport by modulating
            intracellular calcium levels in oviduct smooth muscle.
REFERENCE   4  (bases 1 to 4777)
  AUTHORS   Zhang,G., Shu,Z., Yu,J., Li,J., Yi,P., Wu,B., Deng,D., Yan,S.,
            Li,Y., Ren,D., Hou,Y. and Lan,C.
  TITLE     High ANO1 expression is a prognostic factor and correlated with an
            immunosuppressive tumor microenvironment in pancreatic cancer
  JOURNAL   Front Immunol 15, 1341209 (2024)
   PUBMED   38352864
  REMARK    GeneRIF: High ANO1 expression is a prognostic factor and correlated
            with an immunosuppressive tumor microenvironment in pancreatic
            cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4777)
  AUTHORS   He,C., Dai,F.F., Liu,J.S., Geng,Y.S., Zhou,J.X., Hu,Y.Z., Zheng,B.
            and Wang,S.S.
  TITLE     [Expressions of zinc homeostasis proteins, GPR39 and ANO1 mRNA in
            the sperm of asthenozoospermia patients and their clinical
            significance]
  JOURNAL   Zhonghua Nan Ke Xue 30 (1), 18-25 (2024)
   PUBMED   39046409
  REMARK    GeneRIF: [Expressions of zinc homeostasis proteins, GPR39 and ANO1
            mRNA in the sperm of asthenozoospermia patients and their clinical
            significance].
REFERENCE   6  (bases 1 to 4777)
  AUTHORS   Yang,Y.D., Cho,H., Koo,J.Y., Tak,M.H., Cho,Y., Shim,W.S.,
            Park,S.P., Lee,J., Lee,B., Kim,B.M., Raouf,R., Shin,Y.K. and Oh,U.
  TITLE     TMEM16A confers receptor-activated calcium-dependent chloride
            conductance
  JOURNAL   Nature 455 (7217), 1210-1215 (2008)
   PUBMED   18724360
REFERENCE   7  (bases 1 to 4777)
  AUTHORS   Caputo,A., Caci,E., Ferrera,L., Pedemonte,N., Barsanti,C.,
            Sondo,E., Pfeffer,U., Ravazzolo,R., Zegarra-Moran,O. and
            Galietta,L.J.
  TITLE     TMEM16A, a membrane protein associated with calcium-dependent
            chloride channel activity
  JOURNAL   Science 322 (5901), 590-594 (2008)
   PUBMED   18772398
  REMARK    GeneRIF: results indicate that TMEM16A is an intrinsic constituent
            of the calcium-dependent chloride channel
REFERENCE   8  (bases 1 to 4777)
  AUTHORS   Miwa,S., Nakajima,T., Murai,Y., Takano,Y. and Sugiyama,T.
  TITLE     Mutation assay of the novel gene DOG1 in gastrointestinal stromal
            tumors (GISTs)
  JOURNAL   J Gastroenterol 43 (7), 531-537 (2008)
   PUBMED   18648740
  REMARK    GeneRIF: Based on four cases, the DOG1 gene was found not to be
            mutated in gastrointestinal stromal tumors.
REFERENCE   9  (bases 1 to 4777)
  AUTHORS   Katoh,M. and Katoh,M.
  TITLE     Identification and characterization of TMEM16E and TMEM16F genes in
            silico
  JOURNAL   Int J Oncol 24 (5), 1345-1349 (2004)
   PUBMED   15067359
REFERENCE   10 (bases 1 to 4777)
  AUTHORS   Katoh,M. and Katoh,M.
  TITLE     FLJ10261 gene, located within the CCND1-EMS1 locus on human
            chromosome 11q13, encodes the eight-transmembrane protein
            homologous to C12orf3, C11orf25 and FLJ34272 gene products
  JOURNAL   Int J Oncol 22 (6), 1375-1381 (2003)
   PUBMED   12739008
  REMARK    GeneRIF: This gene, located within the CCND1-EMS1 locus on human
            chromosome 11q13, encodes the eight-transmembrane protein
            homologous to C12orf3, C11orf25 and FLJ34272 gene products and is
            amplified in various cancers
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AP000879.4.
            
            On Feb 18, 2021 this sequence version replaced NM_018043.6.
            
            Transcript Variant: This variant (1) encodes isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY728143.1, AL833582.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000355303.10/ ENSP00000347454.5
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-398               AP000879.4         3863-4260
            399-731             AP000879.4         13298-13630
            732-830             AP000879.4         28612-28710
            831-982             AP000879.4         29545-29696
            983-1037            AP000879.4         31280-31334
            1038-1089           AP000879.4         33899-33950
            1090-1145           AP000879.4         37253-37308
            1146-1187           AP000879.4         42004-42045
            1188-1252           AP000879.4         49896-49960
            1253-1387           AP000879.4         51607-51741
            1388-1548           AP000879.4         57465-57625
            1549-1631           AP000879.4         75256-75338
            1632-1643           AP000879.4         77996-78007
            1644-1715           AP000879.4         78603-78674
            1716-1793           AP000879.4         81457-81534
            1794-1868           AP000879.4         82493-82567
            1869-2070           AP000879.4         86707-86908
            2071-2182           AP000879.4         87168-87279
            2183-2240           AP000879.4         88829-88886
            2241-2341           AP000879.4         91016-91116
            2342-2487           AP000879.4         92788-92933
            2488-2640           AP000879.4         96433-96585
            2641-2693           AP000879.4         105550-105602
            2694-2878           AP000879.4         108048-108232
            2879-2984           AP000879.4         111136-111241
            2985-4777           AP000879.4         113284-115076
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.3"
     gene            1..4777
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /note="anoctamin 1"
                     /db_xref="GeneID:55107"
                     /db_xref="HGNC:HGNC:21625"
                     /db_xref="MIM:610108"
     exon            1..398
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     CDS             291..3251
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /note="isoform a is encoded by transcript variant 1; oral
                     cancer overexpressed 2; transmembrane protein 16A (eight
                     membrane-spanning domains); tumor-amplified and
                     overexpressed sequence 2; discovered on gastrointestinal
                     stromal tumors protein 1; Ca2+-activated Cl- channel;
                     calcium activated chloride channel; anoctamin 1, calcium
                     activated chloride channel"
                     /codon_start=1
                     /product="anoctamin-1 isoform a"
                     /protein_id="NP_060513.5"
                     /db_xref="CCDS:CCDS44663.1"
                     /db_xref="GeneID:55107"
                     /db_xref="HGNC:HGNC:21625"
                     /db_xref="MIM:610108"
                     /translation="MRVNEKYSTLPAEDRSVHIINICAIEDIGYLPSEGTLLNSLSVD
                     PDAECKYGLYFRDGRRKVDYILVYHHKRPSGNRTLVRRVQHSDTPSGARSVKQDHPLP
                     GKGASLDAGSGEPPMDYHEDDKRFRREEYEGNLLEAGLELERDEDTKIHGVGFVKIHA
                     PWNVLCREAEFLKLKMPTKKMYHINETRGLLKKINSVLQKITDPIQPKVAEHRPQTMK
                     RLSYPFSREKQHLFDLSDKDSFFDSKTRSTIVYEILKRTTCTKAKYSMGITSLLANGV
                     YAAAYPLHDGDYNGENVEFNDRKLLYEEWARYGVFYKYQPIDLVRKYFGEKIGLYFAW
                     LGVYTQMLIPASIVGIIVFLYGCATMDENIPSMEMCDQRHNITMCPLCDKTCSYWKMS
                     SACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMRLNYRWDLTGFEEEEEAV
                     KDHPRAEYEARVLEKSLKKESRNKEKRRHIPEESTNKWKQRVKTAMAGVKLTDKVKLT
                     WRDRFPAYLTNLVSIIFMIAVTFAIVLGVIIYRISMAAALAMNSSPSVRSNIRVTVTA
                     TAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSFEERLIFKAFLLKFVNSYTPIFY
                     VAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQLIQNNLFEIG
                     IPKMKKLIRYLKLKQQSPPDHEECVKRKQRYEVDYNLEPFAGLTPEYMEMIIQFGFVT
                     LFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAVRAKDIGIWYNILRGIGKLAV
                     IINAFVISFTSDFIPRLVYLYMYSKNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLG
                     YEVQICRYKDYREPPWSENKYDISKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPD
                     IPKDISQQIHKEKVLMVELFMREEQDKQQLLETWMEKERQKDEPPCNHHNTKACPDSL
                     GSPAPSHAYHGGVL"
     exon            399..731
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            732..830
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            831..982
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            983..1037
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1089
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1090..1145
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1146..1187
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..1252
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..1387
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1388..1548
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1549..1631
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1632..1643
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1644..1715
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1716..1793
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1794..1868
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            1869..2070
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            2071..2182
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            2183..2240
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            2241..2341
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            2342..2487
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            2488..2640
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            2641..2693
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            2694..2878
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            2879..2984
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     exon            2985..4777
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4268..4273
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /note="hexamer: AATAAA"
     polyA_site      4290
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /note="major polyA site"
     regulatory      4655..4660
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /note="hexamer: ATTAAA"
     polyA_site      4680
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
     regulatory      4757..4762
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /note="hexamer: AATAAA"
     polyA_site      4777
                     /gene="ANO1"
                     /gene_synonym="DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A"
                     /note="major polyA site"
ORIGIN      
        1 ggcgtccaag ttcctgacca ggcgcgggcc ggcccgcggg accagcagcc gggtggcggc
       61 gcgatcggcc ccgagaggct caggcgcccc ccgcatcgag cgcgcgggcc gggcgggcca
      121 gggcggcggg cggagcggga ggcggccacg tccccggcgg gcctgggcgc ggggaggccc
      181 ggccccctgc gagcgcgccg cgaacgctgc ggtctccgcc cgcagaggcc gccggggccg
      241 tggatgggga gggcgcgccg cccggcggtc ccagcgcaca ggcggccacg atgagggtca
      301 acgagaagta ctcgacgctc ccggccgagg accgcagcgt ccacatcatc aacatctgcg
      361 ccatcgagga catcggctac ctgccgtccg agggcacgct gctgaactcc ttatctgtgg
      421 accctgatgc cgagtgcaag tatggcctgt acttcaggga cggccggcgc aaggtggact
      481 acatcctggt gtaccatcac aagaggccct cgggcaaccg gaccctggtc aggagggtgc
      541 agcacagcga caccccctct ggggctcgca gcgtcaagca ggaccacccc ctgccgggca
      601 agggggcgtc gctggatgca ggctcggggg agcccccgat ggactaccac gaggatgaca
      661 agcgcttccg cagggaggag tacgagggca acctcctgga ggcgggcctg gagctggagc
      721 gggacgagga cactaaaatc cacggagtcg ggtttgtgaa aatccatgcc ccctggaacg
      781 tgctgtgcag agaggccgag tttctgaaac tgaagatgcc gacgaagaag atgtaccaca
      841 ttaatgagac ccgtggcctc ctgaaaaaaa tcaactctgt gctccagaaa atcacagatc
      901 ccatccagcc caaagtggct gagcacaggc cccagaccat gaagagactc tcctatccct
      961 tctcccggga gaagcagcat ctatttgact tgtctgataa ggattccttt ttcgacagca
     1021 aaacccggag cacgattgtc tatgagatct tgaagagaac gacgtgtaca aaggccaagt
     1081 acagcatggg catcacgagc ctgctggcca atggtgtgta cgcggctgca tacccactgc
     1141 acgatggaga ctacaacggt gaaaacgtcg agttcaacga cagaaaactc ctgtacgaag
     1201 agtgggcacg ctatggagtt ttctataagt accagcccat cgacctggtc aggaagtatt
     1261 ttggggagaa gatcggcctg tacttcgcct ggctgggcgt gtacacccag atgctcatcc
     1321 ctgcctccat cgtgggaatc attgtcttcc tgtacggatg cgccaccatg gatgaaaaca
     1381 tccccagcat ggagatgtgt gaccagagac acaatatcac catgtgcccg ctttgcgaca
     1441 agacctgcag ctactggaag atgagctcag cctgcgccac ggcccgcgcc agccacctct
     1501 tcgacaaccc cgccacggtc ttcttctctg tcttcatggc cctctgggct gccaccttca
     1561 tggagcactg gaagcggaaa cagatgcgac tcaactaccg ctgggacctc acgggctttg
     1621 aagaggaaga ggaggctgtc aaggatcatc ctagagctga atacgaagcc agagtcttgg
     1681 agaagtctct gaagaaagag tccagaaaca aagagaagcg ccggcatatt ccagaggagt
     1741 caacaaacaa atggaagcag agggttaaga cagccatggc gggggtgaaa ttgactgaca
     1801 aagtgaagct gacatggaga gatcggttcc cagcctacct cactaacttg gtctccatca
     1861 tcttcatgat tgcagtgacg tttgccatcg tcctcggcgt catcatctac agaatctcca
     1921 tggccgccgc cttggccatg aactcctccc cctccgtgcg gtccaacatc cgggtcacag
     1981 tcacagccac cgcagtcatc atcaacctag tggtcatcat cctcctggac gaggtgtatg
     2041 gctgcatagc ccgatggctc accaagatcg aggtcccaaa gacggagaaa agctttgagg
     2101 agaggctgat cttcaaggct ttcctgctga agtttgtgaa ttcctacacc cccatctttt
     2161 acgtggcgtt cttcaaaggc cggtttgttg gacgcccggg cgactacgtg tacattttcc
     2221 gttccttccg aatggaagag tgtgcgccag ggggctgcct gatggagcta tgcatccagc
     2281 tcagcatcat catgctgggg aaacagctga tccagaacaa cctgttcgag atcggcatcc
     2341 cgaagatgaa gaagctcatc cgctacctga agctgaagca gcagagcccc cctgaccacg
     2401 aggagtgtgt gaagaggaaa cagcggtacg aggtggatta caacctggag cccttcgcgg
     2461 gcctcacccc agagtacatg gaaatgatca tccagtttgg cttcgtcacc ctgtttgtcg
     2521 cctccttccc cctggcccca ctgtttgcgc tgctgaacaa catcatcgag atccgcctgg
     2581 acgccaaaaa gtttgtcact gagctccgaa ggccggtagc tgtcagagcc aaagacatcg
     2641 gaatctggta caatatcctc agaggcattg ggaagcttgc tgtcatcatc aatgccttcg
     2701 tgatctcctt cacgtctgac ttcatcccgc gcctggtgta cctctacatg tacagtaaga
     2761 acgggaccat gcacggcttc gtcaaccaca ccctctcctc cttcaacgtc agtgacttcc
     2821 agaacggcac ggcccccaat gaccccctgg acctgggcta cgaggtgcag atctgcaggt
     2881 ataaagacta ccgagagccg ccgtggtcgg aaaacaagta cgacatctcc aaggacttct
     2941 gggccgtcct ggcagcccgg ctggcgtttg tcatcgtctt ccagaacctg gtcatgttca
     3001 tgagcgactt tgtggactgg gtcatcccgg acatccccaa ggacatcagc cagcagatcc
     3061 acaaggagaa ggtgctcatg gtggagctgt tcatgcggga ggagcaagac aagcagcagc
     3121 tgctggaaac ctggatggag aaggagcggc agaaggacga gccgccgtgc aaccaccaca
     3181 acaccaaagc ctgcccagac agcctcggca gcccagcccc cagccatgcc taccacgggg
     3241 gcgtcctgta gctatgccag cggggctggg caggccagcc gggcatcctg accgatgggc
     3301 accctctccc agggcaggcg gcttcccgct cccaccaggg cccggtgggt cctgggtttt
     3361 ctgcaaacat ggaggaccac tttctgatag gacattttcc tttcttcttt ctgttttctt
     3421 tcccttgttt ttgcacaaag ccattatgca gggaatattt tttaatctgt agtattcaag
     3481 atgaatcaaa atgatggctg gtaatacggc aataaggtag caaaggcagg tgctttgcag
     3541 aaagaatgct tggaaacttg agtctcccta gaggtgaaaa gtgagcagag gcccgtagaa
     3601 accctcctct gaatcctcct aattccttaa gatagatgca aaatggtaag ccgaggcatc
     3661 gcgcaaaagc tggtgcgatg cttcagggaa aatggaaaac ccacgcaaga ataatgattg
     3721 attccggttc caaaaggtgt cacctacctg tttcagaaaa gttagacttt ccatcgcctt
     3781 ttccttccat cagttgagtg gctgagagag aagtgcctca tccctgagcc acacaggggg
     3841 cgtgggagca tcccagttat ccctggaaag ctagaagggg acagaggtgt ccctgattaa
     3901 gcaggaaaca gcacccttgg cgtccccagc aggctcccca ctgtcagcca cacacctgcc
     3961 cccatcacac caagccgacc tcagagttgt tcatcttcct tatgggacaa aaccggttga
     4021 ccagaaaatg ggcagagaga gatgacctcg gaagcatttc cacagatggt gtcagggttt
     4081 caagaagtct tagggcttcc aggggtcccc tggaagcttt agaatattta tgggtttttt
     4141 ttcaaatatc aattatatgg tagattgagg attttttttc tgtagctcaa aggtggaggg
     4201 agtttattag ttaaccaaat atcgttgaga ggaatttaaa atactgttac taccaaagat
     4261 ttttattaat aaaggcttat attttggtaa cacttctcta tatttttact cacaggaatg
     4321 tcactgttgg acaattattt taaaagtgta taaaaccaag tctcataaat gatatgagtg
     4381 atctaaattt gcagcaatga tactaaacaa ctctctgaaa tttctcaagc accaagagaa
     4441 acatcatttt agcaaaggcc aggaggaaaa atagaaataa atttgtcttg aagatctcat
     4501 tgatgtgatg ttacattccc tttaatctgc caactgtggt caaagttcat aggtgtcgta
     4561 catttccatt atttgctaaa atcatgcaat ctgatgcttc tcttttctct tgtacagtaa
     4621 gtagtttgaa gtgggttttg tatataaata ctgtattaaa aattaggcaa ttaccaaaaa
     4681 tccttttatg gaaaccattt ttttaaaaag tgaatgtaca caaatccaca gaggactgtg
     4741 gctggacatt catctaaata aatttgaata tacgaca
//
