LOCUS       NM_145865               4323 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens ankyrin repeat and sterile alpha motif domain
            containing 4B (ANKS4B), mRNA.
ACCESSION   NM_145865
VERSION     NM_145865.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Graves,M.J., Matoo,S., Choi,M.S., Storad,Z.A., El Sheikh
            Idris,R.A., Pickles,B.K., Acharya,P., Shinder,P.E., Arvay,T.O. and
            Crawley,S.W.
  TITLE     A cryptic sequence targets the adhesion complex scaffold ANKS4B to
            apical microvilli to promote enterocyte brush border assembly
  JOURNAL   J Biol Chem 295 (36), 12588-12604 (2020)
   PUBMED   32636301
  REMARK    GeneRIF: A cryptic sequence targets the adhesion complex scaffold
            ANKS4B to apical microvilli to promote enterocyte brush border
            assembly.
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Choi,M.S., Graves,M.J., Matoo,S., Storad,Z.A., El Sheikh
            Idris,R.A., Weck,M.L., Smith,Z.B., Tyska,M.J. and Crawley,S.W.
  TITLE     The small EF-hand protein CALML4 functions as a critical myosin
            light chain within the intermicrovillar adhesion complex
  JOURNAL   J Biol Chem 295 (28), 9281-9296 (2020)
   PUBMED   32209652
REFERENCE   3  (bases 1 to 4323)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 4323)
  AUTHORS   He,Y., Li,J. and Zhang,M.
  TITLE     Myosin VII, USH1C, and ANKS4B or USH1G Together Form Condensed
            Molecular Assembly via Liquid-Liquid Phase Separation
  JOURNAL   Cell Rep 29 (4), 974-986 (2019)
   PUBMED   31644917
  REMARK    GeneRIF: Myosin VII, USH1C, and ANKS4B or USH1G Together Form
            Condensed Molecular Assembly via Liquid-Liquid Phase Separation.
REFERENCE   5  (bases 1 to 4323)
  AUTHORS   Tomkins,J.E., Dihanich,S., Beilina,A., Ferrari,R., Ilacqua,N.,
            Cookson,M.R., Lewis,P.A. and Manzoni,C.
  TITLE     Comparative Protein Interaction Network Analysis Identifies Shared
            and Distinct Functions for the Human ROCO Proteins
  JOURNAL   Proteomics 18 (10), e1700444 (2018)
   PUBMED   29513927
REFERENCE   6  (bases 1 to 4323)
  AUTHORS   Crawley,S.W., Weck,M.L., Grega-Larson,N.E., Shifrin,D.A. Jr. and
            Tyska,M.J.
  TITLE     ANKS4B Is Essential for Intermicrovillar Adhesion Complex Formation
  JOURNAL   Dev Cell 36 (2), 190-200 (2016)
   PUBMED   26812018
  REMARK    GeneRIF: ankyrin repeat and sterile alpha motif domain containing
            4B (ANKS4B) localizes to the tips of adherent brush border
            microvilli and is essential for intermicrovillar adhesion
REFERENCE   7  (bases 1 to 4323)
  AUTHORS   Reyniers,L., Del Giudice,M.G., Civiero,L., Belluzzi,E.,
            Lobbestael,E., Beilina,A., Arrigoni,G., Derua,R., Waelkens,E.,
            Li,Y., Crosio,C., Iaccarino,C., Cookson,M.R., Baekelandt,V.,
            Greggio,E. and Taymans,J.M.
  TITLE     Differential protein-protein interactions of LRRK1 and LRRK2
            indicate roles in distinct cellular signaling pathways
  JOURNAL   J Neurochem 131 (2), 239-250 (2014)
   PUBMED   24947832
REFERENCE   8  (bases 1 to 4323)
  AUTHORS   Sato,Y., Hatta,M., Karim,M.F., Sawa,T., Wei,F.Y., Sato,S.,
            Magnuson,M.A., Gonzalez,F.J., Tomizawa,K., Akaike,T., Yoshizawa,T.
            and Yamagata,K.
  TITLE     Anks4b, a novel target of HNF4alpha protein, interacts with GRP78
            protein and regulates endoplasmic reticulum stress-induced
            apoptosis in pancreatic beta-cells
  JOURNAL   J Biol Chem 287 (27), 23236-23245 (2012)
   PUBMED   22589549
REFERENCE   9  (bases 1 to 4323)
  AUTHORS   Johnston,A.M., Naselli,G., Niwa,H., Brodnicki,T., Harrison,L.C. and
            Gonez,L.J.
  TITLE     Harp (harmonin-interacting, ankyrin repeat-containing protein), a
            novel protein that interacts with harmonin in epithelial tissues
  JOURNAL   Genes Cells 9 (10), 967-982 (2004)
   PUBMED   15461667
REFERENCE   10 (bases 1 to 4323)
  AUTHORS   Weil,D., El-Amraoui,A., Masmoudi,S., Mustapha,M., Kikkawa,Y.,
            Laine,S., Delmaghani,S., Adato,A., Nadifi,S., Zina,Z.B., Hamel,C.,
            Gal,A., Ayadi,H., Yonekawa,H. and Petit,C.
  TITLE     Usher syndrome type I G (USH1G) is caused by mutations in the gene
            encoding SANS, a protein that associates with the USH1C protein,
            harmonin
  JOURNAL   Hum Mol Genet 12 (5), 463-471 (2003)
   PUBMED   12588794
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK096138.1, BC112368.1 and AF001550.1.
            
            On Nov 22, 2018 this sequence version replaced NM_145865.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK096138.1, BC111784.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000311620.7/ ENSP00000308772.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1290              AK096138.1         5-1294
            1291-1636           BC112368.1         1293-1638
            1637-1637           AF001550.1         118589-118589
            1638-2011           BC112368.1         1640-2013
            2012-2048           AK096138.1         2016-2052
            2049-2049           AF001550.1         119001-119001
            2050-2420           AK096138.1         2054-2424
            2421-2422           AF001550.1         119373-119374
            2423-2484           AK096138.1         2425-2486
            2485-2485           AF001550.1         119437-119437
            2486-2902           AK096138.1         2488-2904
            2903-4323           AF001550.1         119855-121275
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p12.2"
     gene            1..4323
                     /gene="ANKS4B"
                     /gene_synonym="HARP"
                     /note="ankyrin repeat and sterile alpha motif domain
                     containing 4B"
                     /db_xref="GeneID:257629"
                     /db_xref="HGNC:HGNC:26795"
                     /db_xref="MIM:609901"
     exon            1..203
                     /gene="ANKS4B"
                     /gene_synonym="HARP"
                     /inference="alignment:Splign:2.1.0"
     CDS             40..1293
                     /gene="ANKS4B"
                     /gene_synonym="HARP"
                     /note="harmonin-interacting ankyrin-repeat containing
                     protein"
                     /codon_start=1
                     /product="ankyrin repeat and SAM domain-containing protein
                     4B"
                     /protein_id="NP_665872.2"
                     /db_xref="CCDS:CCDS42130.1"
                     /db_xref="GeneID:257629"
                     /db_xref="HGNC:HGNC:26795"
                     /db_xref="MIM:609901"
                     /translation="MSTRYHQAASDSYLELLKEATKRDLNLSDEDGMTPTLLAAYHGN
                     LEALEIICSRGGDPDRCDIWGNTPLHFAASNGHAHCVSFLVNFGANIFALDNDLQTPL
                     DAAASREQNECVALLDKAATAQNIMNPKKVTRLKEQAQKNARRQIKECERLQEKHQNK
                     MAHTYSKEESGTLSSSKGTFSRSSPSNASAPGTFGSLSKGIKDTFKIKFKKNKDTAEQ
                     VGKEGRSGQRNVMEVFREEEEDSFSGDFKEKLQLSAEEDGSVHHESILNRPGLGSIVF
                     RRNRISSPEDISDSKREFGFKLPSELLQRQGASEADEGAADEEGEENGLKDDLPWDDD
                     EVEWEEDVVDATPLEVFLLSQHLEEFLPIFKREQIDLEALLLCSDEDLQSIQMQLGPR
                     KKVLNAINRRKQVLQQPGQLVDTSL"
     exon            204..4323
                     /gene="ANKS4B"
                     /gene_synonym="HARP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2878..2883
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANKS4B"
                     /gene_synonym="HARP"
                     /note="hexamer: AATAAA"
     regulatory      2892..2897
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANKS4B"
                     /gene_synonym="HARP"
                     /note="hexamer: GATAAA"
     polyA_site      2901
                     /gene="ANKS4B"
                     /gene_synonym="HARP"
                     /note="major polyA site"
     polyA_site      2915
                     /gene="ANKS4B"
                     /gene_synonym="HARP"
                     /note="major polyA site"
     regulatory      3835..3840
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANKS4B"
                     /gene_synonym="HARP"
                     /note="hexamer: AATAAA"
     polyA_site      3857
                     /gene="ANKS4B"
                     /gene_synonym="HARP"
     regulatory      3887..3892
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANKS4B"
                     /gene_synonym="HARP"
                     /note="hexamer: AATAAA"
     polyA_site      3914
                     /gene="ANKS4B"
                     /gene_synonym="HARP"
     regulatory      4298..4303
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANKS4B"
                     /gene_synonym="HARP"
                     /note="hexamer: AATAAA"
     polyA_site      4323
                     /gene="ANKS4B"
                     /gene_synonym="HARP"
ORIGIN      
        1 ctggagagac atctggccaa gttctggtga gcaggaaaaa tgtctactcg ttaccaccaa
       61 gctgctagtg atagttacct ggaacttcta aaagaggcta ccaagcgaga tctaaatctt
      121 tcggatgaag acggcatgac tcctactctc ttggcagcct accatgggaa cttggaagcc
      181 ctagagataa tctgcagtag aggaggggac cctgataggt gtgacatctg gggaaacact
      241 cctctacatt ttgcagcctc caatggccat gcccactgcg tctcattcct ggtcaacttt
      301 ggtgccaaca tctttgccct ggataatgac ttacagactc cactggatgc tgctgccagc
      361 agggagcaga atgaatgtgt tgctctcctg gacaaggctg ccactgcaca gaacatcatg
      421 aaccccaaga aggtcaccag gctgaaggag caggctcaga agaatgccag gaggcagatc
      481 aaagagtgtg agaggctcca ggagaagcac caaaataaga tggcccacac ctacagcaag
      541 gaggaatccg ggactctctc ttcttccaag ggtaccttct ccagatcatc cccttcaaat
      601 gcttctgctc ctggcacatt cgggtcacta tctaagggca ttaaagacac tttcaagatc
      661 aagttcaaga agaacaaaga tacagcagaa caggtgggga aggaaggcag aagtgggcag
      721 aggaacgtga tggaagtgtt cagagaggaa gaggaagact cgttctcagg ggacttcaaa
      781 gagaagctcc agttgtcagc agaggaggac ggcagtgtgc accatgaatc cattctcaat
      841 cgtccaggtc taggaagtat tgtttttaga aggaacagga tatcgagtcc tgaagacatc
      901 tcagatagca agagagagtt tggttttaaa ctgcccagtg aattgcttca aagacaagga
      961 gcatcagagg ctgatgaggg tgcagctgat gaagagggag aggaaaacgg cctcaaagat
     1021 gatctgccgt gggatgacga tgaagtggag tgggaggaag atgtggtcga tgccacgccc
     1081 ctggaagtgt tcttgctgtc tcagcacctg gaagaattcc tgcctatctt caagagagag
     1141 cagattgatc tagaagctct gctgctctgc tctgatgagg accttcagag catacaaatg
     1201 cagctgggtc ccaggaagaa agttctgaat gctatcaaca ggaggaagca ggtgcttcaa
     1261 cagcctgggc agctggtcga caccagcctg tgatggagag ttttggcctg gagcattggg
     1321 gtgatgctgt ggcccgctgg cagcactcca ggcggcaccc cctctttacc caatgccaga
     1381 ccactgggaa tggattctag ggcatcggaa atgcctacct gagagagaga cccaaacttt
     1441 actctgggag gtaggctatg cccatccaaa taaatctcca tgagaaactt gaggagactt
     1501 cataacaaga atctggcatt tctcttcagt tatcttatat gtacatataa ttgtttttgt
     1561 ggttgttttg ttttgttttg ttttgttttt tggagatgaa ggtctcagtc tcttacccag
     1621 gctagggtgc agtggtatga tcatagttca ctgtattctc aacctcctgg gctcaaatga
     1681 tctcctccca cctcagcctc ccaagtagct gagactacag gttcacaccc cccacacctg
     1741 gcttattttg tatgttttag tagaggtggg gtcttgccac attgcccagg ctggtctcaa
     1801 actcctggcc tcaagcaatc ctcccacctc agcctcctaa agcactggga ttacaggtgt
     1861 gaaccaccgt acccagccta tctttttgat acttttgaat aaagaaaggg tcatatgcat
     1921 gacaggaaaa tgaaagaaac ttcctttact tttctatctc tggatttaaa attataatct
     1981 catcacatta tcctgctgct tgctttccga tctgtgtaac ctgggaattc caattctttt
     2041 tctctcctga gatctatgac tttgcctagt ggtagagact agagttcttt cctggcctgc
     2101 ggcttgatgc ccaacttaaa tgcatctaac cctttaacaa atgtgtacat gtttacaagt
     2161 aatggaaatg cgtctataat actcctgcct gagaatagag acagagtggt ggtggggaga
     2221 gtgaagaaag agatagaata caggtggtac ctgttgtgga ctgaattgcg tcaaattcat
     2281 atgttggagc tctaacccct aatgtgactg taattggaaa taagaccttt aaagaagtga
     2341 ttaaggttaa atgaagtcat aagaatgcaa ccctaatcct gtaggactgg tgtccttttt
     2401 ttcccttttt tttttttttt ttgagatgga gccttgctct gtcactcatg ctggagtgca
     2461 gtggcgtgat ctcagctcac tgcaaccccc gcctcccagg ttcgagcact tttcatgcct
     2521 cagcctcctg agtagctggg attacaggcg tgcaccacaa cgcctggcta agtttttgta
     2581 tttttagtag aggcggggtt tcaccatgtt ggccaggctg gtctcaaact cctgacctca
     2641 ggtgatccac ctgcctcggc ctcccagagt gctgggatta caggcatgag ccactgcacc
     2701 tggcctagga ctggtgtcct aagaagagga agagacactt aggtggaagg cacacagaga
     2761 ggccacgtga ggacacagtg agaaggtggc cgtctgcaag ccgaggaggg ggcctcagga
     2821 gaaaccaacc ctgcaatcac cttgatcttg ggctttcagc ccccaaaggt gtgagaaaat
     2881 aaacttcggt tgataaactg ctgttgttga agccatccag tctgtggcat tttgttatgg
     2941 cagtcctagc agaataatac tgtcctaagt aagagggttg gggaggagac caagaaaaat
     3001 acagaaaaaa agtctgtcca actgcaattg atgagttttg taagggtaaa cacctagtga
     3061 aacttaaggg gaaaaaaaac taagttcttt ggagggaaga tttgattgtc aaaggaaatt
     3121 tcacattttc atgcttatta tgtacacatg gtttatttac tgttgtctgt caccattgcc
     3181 gcatatctga atatgtgtag gttccacgat agaaactgac aacacttggc tcatgcctgt
     3241 aatcccagca ctttgggagg cccaggcagg cagatcacct gaggtcagga gttcaagacc
     3301 agcctggcca acatggcgga aacccgtctc tactaaaaat acaaaaatta gccaggtgtg
     3361 gtggcgtgtg cctgtaatcc cagctacttg ggaggctgag gcaagagaat tgcttgaacc
     3421 caaaaggtgg aggttgcagt gagttgagat tgcaccactg cactctagcc caggcggtaa
     3481 gagagactcc atctcaaaaa aaaaaaaaaa aaaaaagaaa aaagaaactg acaacactgc
     3541 tgctgacatt ttttcaatgg caatcccaaa ttcaaactga aaccccactg aagagctaag
     3601 cttcattaga tctctacagg ctgacttaca tcaagtggaa tttactgttg attctgggta
     3661 taatacagaa acagctgttt atcttcagct tgctttctga tgcacatctg tttgggttac
     3721 ttcaagaggc atcatggagg attcaagttt agggaggata cagatgctca aatctgatga
     3781 acaattggct tattcttcct caatgaatat attcagaaag cttgttagcc attgaataaa
     3841 cacgttgcat taggggatga ttgtttacaa ataccttatc ttgtggaata aactgaagtt
     3901 gtgctttcct tcattaacgt gcacagaagc agttggcaaa tagaaagtgc tcaaaaaagt
     3961 ttgtagtctg tgcctgccac tattatattt catcatcata gatgccagaa tcccctcttc
     4021 acctattgcc cggaatctgc ctaatgagat atttgctcca ccccctgctg taactacatc
     4081 atagcaagag agccatcact caggttggta gaaatgaact tgaacattga ctctcatccc
     4141 tttttaggag atatttgcca ctaggggtct catcaggtac ctctatgctc ttcttagggg
     4201 ttcttttctt ttatgattga cagaattaat aggtgagcgt tctccaaagt tctcagcatc
     4261 aaggatatag tctcttcgtc acagagtatt gttatataat aaaaaaaggt ttataccatg
     4321 tta
//
