LOCUS       NP_665872                417 aa            linear   PRI 27-APR-2025
DEFINITION  ankyrin repeat and SAM domain-containing protein 4B [Homo sapiens].
ACCESSION   NP_665872
VERSION     NP_665872.2
DBSOURCE    REFSEQ: accession NM_145865.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 417)
  AUTHORS   Graves,M.J., Matoo,S., Choi,M.S., Storad,Z.A., El Sheikh
            Idris,R.A., Pickles,B.K., Acharya,P., Shinder,P.E., Arvay,T.O. and
            Crawley,S.W.
  TITLE     A cryptic sequence targets the adhesion complex scaffold ANKS4B to
            apical microvilli to promote enterocyte brush border assembly
  JOURNAL   J Biol Chem 295 (36), 12588-12604 (2020)
   PUBMED   32636301
  REMARK    GeneRIF: A cryptic sequence targets the adhesion complex scaffold
            ANKS4B to apical microvilli to promote enterocyte brush border
            assembly.
REFERENCE   2  (residues 1 to 417)
  AUTHORS   Choi,M.S., Graves,M.J., Matoo,S., Storad,Z.A., El Sheikh
            Idris,R.A., Weck,M.L., Smith,Z.B., Tyska,M.J. and Crawley,S.W.
  TITLE     The small EF-hand protein CALML4 functions as a critical myosin
            light chain within the intermicrovillar adhesion complex
  JOURNAL   J Biol Chem 295 (28), 9281-9296 (2020)
   PUBMED   32209652
REFERENCE   3  (residues 1 to 417)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (residues 1 to 417)
  AUTHORS   He,Y., Li,J. and Zhang,M.
  TITLE     Myosin VII, USH1C, and ANKS4B or USH1G Together Form Condensed
            Molecular Assembly via Liquid-Liquid Phase Separation
  JOURNAL   Cell Rep 29 (4), 974-986 (2019)
   PUBMED   31644917
  REMARK    GeneRIF: Myosin VII, USH1C, and ANKS4B or USH1G Together Form
            Condensed Molecular Assembly via Liquid-Liquid Phase Separation.
REFERENCE   5  (residues 1 to 417)
  AUTHORS   Tomkins,J.E., Dihanich,S., Beilina,A., Ferrari,R., Ilacqua,N.,
            Cookson,M.R., Lewis,P.A. and Manzoni,C.
  TITLE     Comparative Protein Interaction Network Analysis Identifies Shared
            and Distinct Functions for the Human ROCO Proteins
  JOURNAL   Proteomics 18 (10), e1700444 (2018)
   PUBMED   29513927
REFERENCE   6  (residues 1 to 417)
  AUTHORS   Crawley,S.W., Weck,M.L., Grega-Larson,N.E., Shifrin,D.A. Jr. and
            Tyska,M.J.
  TITLE     ANKS4B Is Essential for Intermicrovillar Adhesion Complex Formation
  JOURNAL   Dev Cell 36 (2), 190-200 (2016)
   PUBMED   26812018
  REMARK    GeneRIF: ankyrin repeat and sterile alpha motif domain containing
            4B (ANKS4B) localizes to the tips of adherent brush border
            microvilli and is essential for intermicrovillar adhesion
REFERENCE   7  (residues 1 to 417)
  AUTHORS   Reyniers,L., Del Giudice,M.G., Civiero,L., Belluzzi,E.,
            Lobbestael,E., Beilina,A., Arrigoni,G., Derua,R., Waelkens,E.,
            Li,Y., Crosio,C., Iaccarino,C., Cookson,M.R., Baekelandt,V.,
            Greggio,E. and Taymans,J.M.
  TITLE     Differential protein-protein interactions of LRRK1 and LRRK2
            indicate roles in distinct cellular signaling pathways
  JOURNAL   J Neurochem 131 (2), 239-250 (2014)
   PUBMED   24947832
REFERENCE   8  (residues 1 to 417)
  AUTHORS   Sato,Y., Hatta,M., Karim,M.F., Sawa,T., Wei,F.Y., Sato,S.,
            Magnuson,M.A., Gonzalez,F.J., Tomizawa,K., Akaike,T., Yoshizawa,T.
            and Yamagata,K.
  TITLE     Anks4b, a novel target of HNF4alpha protein, interacts with GRP78
            protein and regulates endoplasmic reticulum stress-induced
            apoptosis in pancreatic beta-cells
  JOURNAL   J Biol Chem 287 (27), 23236-23245 (2012)
   PUBMED   22589549
REFERENCE   9  (residues 1 to 417)
  AUTHORS   Johnston,A.M., Naselli,G., Niwa,H., Brodnicki,T., Harrison,L.C. and
            Gonez,L.J.
  TITLE     Harp (harmonin-interacting, ankyrin repeat-containing protein), a
            novel protein that interacts with harmonin in epithelial tissues
  JOURNAL   Genes Cells 9 (10), 967-982 (2004)
   PUBMED   15461667
REFERENCE   10 (residues 1 to 417)
  AUTHORS   Weil,D., El-Amraoui,A., Masmoudi,S., Mustapha,M., Kikkawa,Y.,
            Laine,S., Delmaghani,S., Adato,A., Nadifi,S., Zina,Z.B., Hamel,C.,
            Gal,A., Ayadi,H., Yonekawa,H. and Petit,C.
  TITLE     Usher syndrome type I G (USH1G) is caused by mutations in the gene
            encoding SANS, a protein that associates with the USH1C protein,
            harmonin
  JOURNAL   Hum Mol Genet 12 (5), 463-471 (2003)
   PUBMED   12588794
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK096138.1, BC112368.1 and AF001550.1.
            
            On Jun 7, 2007 this sequence version replaced NP_665872.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK096138.1, BC111784.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000311620.7/ ENSP00000308772.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p12.2"
     Protein         1..417
                     /product="ankyrin repeat and SAM domain-containing protein
                     4B"
                     /note="harmonin-interacting ankyrin-repeat containing
                     protein"
                     /calculated_mol_wt=46466
     Region          1..252
                     /region_name="Mediates localization to microvilli.
                     /evidence=ECO:0000269|PubMed:26812018"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8N8V4.2)"
     Region          <1..163
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          3..29
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(6..7,10..12,14..15,19,22,29,31,33,37..38,41..43,
                     45..46,50,53,62,64,66,70..71,74..76,78..79,83,86,95)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          31..62
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          64..95
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          97..125
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          151..195
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8N8V4.2)"
     Region          253..346
                     /region_name="Mediates interaction with MYO7B.
                     /evidence=ECO:0000269|PubMed:26812018"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8N8V4.2)"
     Region          255..>281
                     /region_name="CEN_ANKS4B"
                     /note="central domain found in ankyrin repeat and SAM
                     domain-containing protein 4B; cd21802"
                     /db_xref="CDD:409641"
     Site            order(264..265,268..269,271..276)
                     /site_type="other"
                     /note="myosin tail binding site [polypeptide binding]"
                     /db_xref="CDD:409641"
     Site            283
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:24275569; propagated from
                     UniProtKB/Swiss-Prot (Q8N8V4.2)"
     Region          305..330
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8N8V4.2)"
     Region          345..410
                     /region_name="SAM_HARP"
                     /note="SAM domain of HARP subfamily; cd09587"
                     /db_xref="CDD:188986"
     Site            order(345,347,349..351,353..354,356,401..402,406)
                     /site_type="other"
                     /note="putative polypeptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:188986"
     Region          415..417
                     /region_name="PDZ-binding, mediates interaction with
                     USH1C. /evidence=ECO:0000269|PubMed:26812018"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8N8V4.2)"
     CDS             1..417
                     /gene="ANKS4B"
                     /gene_synonym="HARP"
                     /coded_by="NM_145865.3:40..1293"
                     /db_xref="CCDS:CCDS42130.1"
                     /db_xref="GeneID:257629"
                     /db_xref="HGNC:HGNC:26795"
                     /db_xref="MIM:609901"
ORIGIN      
        1 mstryhqaas dsylellkea tkrdlnlsde dgmtptllaa yhgnlealei icsrggdpdr
       61 cdiwgntplh faasnghahc vsflvnfgan ifaldndlqt pldaaasreq necvalldka
      121 ataqnimnpk kvtrlkeqaq knarrqikec erlqekhqnk mahtyskees gtlssskgtf
      181 srsspsnasa pgtfgslskg ikdtfkikfk knkdtaeqvg kegrsgqrnv mevfreeeed
      241 sfsgdfkekl qlsaeedgsv hhesilnrpg lgsivfrrnr isspedisds krefgfklps
      301 ellqrqgase adegaadeeg eenglkddlp wdddevewee dvvdatplev fllsqhleef
      361 lpifkreqid lealllcsde dlqsiqmqlg prkkvlnain rrkqvlqqpg qlvdtsl
//
