LOCUS       NM_052997               4664 bp    mRNA    linear   PRI 12-JUN-2024
DEFINITION  Homo sapiens ankyrin repeat domain 30A (ANKRD30A), mRNA.
ACCESSION   NM_052997 XM_940707
VERSION     NM_052997.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Sun,L., Li,P., Ren,H., Liu,G. and Sun,L.
  TITLE     A four-gene expression-based signature predicts the clinical
            outcome of melanoma
  JOURNAL   J BUON 24 (5), 2161-2167 (2019)
   PUBMED   31786890
  REMARK    GeneRIF: This observation was confirmed in four independent
            cohorts, GSE22138, GSE54467, GSE65904 and E-MTAB-4725. In addition,
            the melanoma score was independent of most clinically used
            indicators.
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Biserni,G.B., Di Oto,E., Moskovszky,L.E., Foschini,M.P. and
            Varga,Z.
  TITLE     Preferential expression of NY-BR-1 and GATA-3 in male breast cancer
  JOURNAL   J Cancer Res Clin Oncol 144 (2), 199-204 (2018)
   PUBMED   29116378
  REMARK    GeneRIF: Authors found a high sensitivity for all the markers
            analyzed. Moreover, the expression of NY-BR-1 and GATA-3 seemed the
            most effective for labelling male breast cancer in primary and
            metastatic setting.
REFERENCE   3  (bases 1 to 4664)
  AUTHORS   Kosaloglu,Z., Bitzer,J., Halama,N., Huang,Z., Zapatka,M.,
            Schneeweiss,A., Jager,D. and Zornig,I.
  TITLE     In silico SNP analysis of the breast cancer antigen NY-BR-1
  JOURNAL   BMC Cancer 16 (1), 901 (2016)
   PUBMED   27863482
  REMARK    GeneRIF: Over 2800 SNPs are recorded in the dbSNP and NHLBI Exome
            Sequencing Project (ESP) databases for the NY-BR-1 gene.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4664)
  AUTHORS   Abu El-Nazar,S.Y., Ghazy,A.A., Ghoneim,H.E., Zoheir,M., Ahmed,A.S.,
            Sorour,S.S. and Abouelella,A.M.
  TITLE     NY-BR-1 Antigen Expression and anti-NY-BR-1 IgG in Egyptian Breast
            Cancer Patients: Clinicopathological and Prognostic Significance
  JOURNAL   Egypt J Immunol 22 (1), 1-8 (2015)
   PUBMED   26415367
  REMARK    GeneRIF: Results revealed a significant difference in NY-BR-1 Ag
            expression between benign and malignant breast cancer patients
REFERENCE   5  (bases 1 to 4664)
  AUTHORS   Theurillat,J.P., Zurrer-Hardi,U., Varga,Z., Barghorn,A., Saller,E.,
            Frei,C., Storz,M., Behnke,S., Seifert,B., Fehr,M., Fink,D.,
            Rageth,C., Linsenmeier,C., Pestalozzi,B., Chen,Y.T., Knuth,A.,
            Jager,D. and Moch,H.
  TITLE     Distinct expression patterns of the immunogenic differentiation
            antigen NY-BR-1 in normal breast, testis and their malignant
            counterparts
  JOURNAL   Int J Cancer 122 (7), 1585-1591 (2008)
   PUBMED   18041742
  REMARK    GeneRIF: We identified estrogen-response element (ERE)-like
            sequences nearby the NY-BR-1 promoter region, suggesting that
            NY-BR-1 expression might be regulated through estrogen receptors.
REFERENCE   6  (bases 1 to 4664)
  AUTHORS   Seil,I., Frei,C., Sultmann,H., Knauer,S.K., Engels,K., Jager,E.,
            Zatloukal,K., Pfreundschuh,M., Knuth,A., Tseng-Chen,Y.,
            Jungbluth,A.A., Stauber,R.H. and Jager,D.
  TITLE     The differentiation antigen NY-BR-1 is a potential target for
            antibody-based therapies in breast cancer
  JOURNAL   Int J Cancer 120 (12), 2635-2642 (2007)
   PUBMED   17330232
  REMARK    GeneRIF: The strong expression of NY-BR-1 in breast tumors, its
            cytoplasmic and membrane localization and accessibility to an
            ectopically applied antibody now suggest to pursue NY-BR-1 as a
            potential target for antibody-based therapies in breast cancer
            patients.
REFERENCE   7  (bases 1 to 4664)
  AUTHORS   Jager,D., Filonenko,V., Gout,I., Frosina,D., Eastlake-Wade,S.,
            Castelli,S., Varga,Z., Moch,H., Chen,Y.T., Busam,K.J., Seil,I.,
            Old,L.J., Nissan,A., Frei,C., Gure,A.O., Knuth,A. and
            Jungbluth,A.A.
  TITLE     NY-BR-1 is a differentiation antigen of the mammary gland
  JOURNAL   Appl Immunohistochem Mol Morphol 15 (1), 77-83 (2007)
   PUBMED   17536312
  REMARK    GeneRIF: testing demonstrates NY-BR-1 in a mostly focal fashion in
            the epithelia of ducts and acini of the mammary gland. In tumors,
            homogenous staining can be seen in almost all ductal carcinomas in
            situ and/or the intraductal component of invasive carcinomas.
REFERENCE   8  (bases 1 to 4664)
  AUTHORS   Varga,Z., Theurillat,J.P., Filonenko,V., Sasse,B., Odermatt,B.,
            Jungbluth,A.A., Chen,Y.T., Old,L.J., Knuth,A., Jager,D. and Moch,H.
  TITLE     Preferential nuclear and cytoplasmic NY-BR-1 protein expression in
            primary breast cancer and lymph node metastases
  JOURNAL   Clin Cancer Res 12 (9), 2745-2751 (2006)
   PUBMED   16675566
  REMARK    GeneRIF: NY-BR-1 is a differentiation antigen of the breast, which
            is present in normal and tumorous mammary epithelium.
REFERENCE   9  (bases 1 to 4664)
  AUTHORS   Jager,D., Karbach,J., Pauligk,C., Seil,I., Frei,C., Chen,Y.T.,
            Old,L.J., Knuth,A. and Jager,E.
  TITLE     Humoral and cellular immune responses against the breast cancer
            antigen NY-BR-1: definition of two HLA-A2 restricted peptide
            epitopes
  JOURNAL   Cancer Immun 5, 11 (2005)
   PUBMED   16335914
  REMARK    GeneRIF: In this study we extended the expression analysis to
            various other normal and tumor tissues and found inconsistent
            expression of NY-BR-1 in normal prostate, but consistent NY-BR-1
            mRNA expression in 32% of prostate cancers, but not in other tumor
            types
            Publication Status: Online-Only
REFERENCE   10 (bases 1 to 4664)
  AUTHORS   Jager,D., Stockert,E., Gure,A.O., Scanlan,M.J., Karbach,J.,
            Jager,E., Knuth,A., Old,L.J. and Chen,Y.T.
  TITLE     Identification of a tissue-specific putative transcription factor
            in breast tissue by serological screening of a breast cancer
            library
  JOURNAL   Cancer Res 61 (5), 2055-2061 (2001)
   PUBMED   11280766
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC067744.6, AL357148.22 and
            AL157387.9.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Oct 9, 2020 this sequence version replaced NM_052997.2.
            
            Summary: This gene encodes a DNA-binding transcription factor that
            is uniquely expressed in mammary epithelium and the testis. Altered
            expression levels have been associated with breast cancer
            progression. [provided by RefSeq, Nov 2016].
            
            Sequence Note: This transcript contains splicing in the 3' UTR that
            makes it a candidate for nonsense-mediated mRNA decay (NMD).
            Limited transcript data is available to validate the splice
            pattern, and alternate transcripts and proteins may be expressed in
            vivo.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF269087.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMEA1968968,
                                           SAMEA2148874 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000361713.2/ ENSP00000354432.2
            NMD candidate          :: PMID: 16675566, 17536312
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-411               AC067744.6         128763-129173
            412-526             AC067744.6         133058-133172
            527-700             AC067744.6         133370-133543
            701-807             AC067744.6         135405-135511
            808-945             AL357148.22        566-703
            946-1010            AL357148.22        3257-3321
            1011-1583           AL357148.22        8368-8940
            1584-1645           AL357148.22        11645-11706
            1646-1733           AL357148.22        14020-14107
            1734-1762           AL357148.22        16301-16329
            1763-1835           AL357148.22        16427-16499
            1836-1897           AL357148.22        18710-18771
            1898-1988           AL357148.22        20222-20312
            1989-2017           AL357148.22        25042-25070
            2018-2090           AL357148.22        25164-25236
            2091-2119           AL357148.22        29299-29327
            2120-2192           AL357148.22        29426-29498
            2193-2254           AL357148.22        31744-31805
            2255-2345           AL357148.22        33255-33345
            2346-2374           AL357148.22        36180-36208
            2375-2447           AL357148.22        36302-36374
            2448-2476           AL357148.22        40362-40390
            2477-2549           AL357148.22        40489-40561
            2550-2611           AL357148.22        42807-42868
            2612-2702           AL357148.22        56117-56207
            2703-2731           AL357148.22        59714-59742
            2732-2804           AL357148.22        59836-59908
            2805-2833           AL357148.22        63931-63959
            2834-2906           AL357148.22        64058-64130
            2907-2968           AL357148.22        66377-66438
            2969-3059           AL357148.22        67885-67975
            3060-3273           AL357148.22        82831-83044
            3274-3457           AL357148.22        84345-84528
            3458-3488           AL357148.22        85630-85660
            3489-4375           AL157387.9         2001-2887
            4376-4595           AL157387.9         14451-14670
            4596-4664           AL157387.9         15489-15557
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p11.21"
     gene            1..4664
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /note="ankyrin repeat domain 30A"
                     /db_xref="GeneID:91074"
                     /db_xref="HGNC:HGNC:17234"
                     /db_xref="MIM:610856"
     exon            1..411
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             191..4384
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /note="serologically defined breast cancer antigen
                     NY-BR-1"
                     /codon_start=1
                     /product="ankyrin repeat domain-containing protein 30A"
                     /protein_id="NP_443723.3"
                     /db_xref="CCDS:CCDS7193.2"
                     /db_xref="GeneID:91074"
                     /db_xref="HGNC:HGNC:17234"
                     /db_xref="MIM:610856"
                     /translation="MEEISAAAVKVVPGPERPSPFSQLVYTSNDSYIVHSGDLRKIHK
                     AASRGQVRKLEKMTKRKKTINLNIQDAQKRTALHWACVNGHEEVVTFLVDRKCQLDVL
                     DGEHRTPLMKALQCHQEACANILIDSGADINLVDVYGNTALHYAVYSEILSVVAKLLS
                     HGAVIEVHNKASLTPLLLSITKRSEQIVEFLLIKNANANAVNKYKCTALMLAVCHGSS
                     EIVGMLLQQNVDVFAADICGVTAEHYAVTCGFHHIHEQIMEYIRKLSKNHQNTNPEGT
                     SAGTPDEAAPLAERTPDTAESLVEKTPDEAAPLVERTPDTAESLVEKTPDEAASLVEG
                     TSDKIQCLEKATSGKFEQSAEETPREITSPAKETSEKFTWPAKGRPRKIAWEKKEDTP
                     REIMSPAKETSEKFTWAAKGRPRKIAWEKKETPVKTGCVARVTSNKTKVLEKGRSKMI
                     ACPTKESSTKASANDQRFPSESKQEEDEEYSCDSRSLFESSAKIQVCIPESIYQKVME
                     INREVEEPPKKPSAFKPAIEMQNSVPNKAFELKNEQTLRADPMFPPESKQKDYEENSW
                     DSESLCETVSQKDVCLPKATHQKEIDKINGKLEESPNKDGLLKATCGMKVSIPTKALE
                     LKDMQTFKAEPPGKPSAFEPATEMQKSVPNKALELKNEQTLRADEILPSESKQKDYEE
                     NSWDTESLCETVSQKDVCLPKAAHQKEIDKINGKLEGSPVKDGLLKANCGMKVSIPTK
                     ALELMDMQTFKAEPPEKPSAFEPAIEMQKSVPNKALELKNEQTLRADEILPSESKQKD
                     YEESSWDSESLCETVSQKDVCLPKATHQKEIDKINGKLEESPDNDGFLKAPCRMKVSI
                     PTKALELMDMQTFKAEPPEKPSAFEPAIEMQKSVPNKALELKNEQTLRADQMFPSESK
                     QKKVEENSWDSESLRETVSQKDVCVPKATHQKEMDKISGKLEDSTSLSKILDTVHSCE
                     RARELQKDHCEQRTGKMEQMKKKFCVLKKKLSEAKEIKSQLENQKVKWEQELCSVRLT
                     LNQEEEKRRNADILNEKIREELGRIEEQHRKELEVKQQLEQALRIQDIELKSVESNLN
                     QVSHTHENENYLLHENCMLKKEIAMLKLEIATLKHQYQEKENKYFEDIKILKEKNAEL
                     QMTLKLKEESLTKRASQYSGQLKVLIAENTMLTSKLKEKQDKEILEAEIESHHPRLAS
                     AVQDHDQIVTSRKSQEPAFHIAGDACLQRKMNVDVSSTIYNNEVLHQPLSEAQRKSKS
                     LKINLNYAGDALRENTLVSEHAQRDQRETQCQMKEAEHMYQNEQDNVNKHTEQQESLD
                     QKLFQLQSKNMWLQQQLVHAHKKADNKSKITIDIHFLERKMQHHLLKEKNEEIFNYNN
                     HLKNRIYQYEKEKAETENS"
     exon            412..526
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            527..700
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            701..807
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            808..945
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            946..1010
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1011..1583
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1584..1645
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1646..1733
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1734..1762
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1763..1835
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..1897
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1898..1988
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1989..2017
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2018..2090
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2091..2119
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2120..2192
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2193..2254
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2255..2345
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2346..2374
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2375..2447
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2448..2476
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2477..2549
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2550..2611
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2612..2702
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2703..2731
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2732..2804
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2805..2833
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2834..2906
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2907..2968
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2969..3059
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3060..3273
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3274..3457
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3458..4375
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4376..4595
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4596..4664
                     /gene="ANKRD30A"
                     /gene_synonym="NY-BR-1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggctctgctc agcgcgattc tactgagaga ggcctgagtg tgcaagagct tggcgaacac
       61 agaacttttt acgggtatcg aggcggtgcg tggactgaag aagggcgagg gcgattgggg
      121 aggggtgggg ggtggtggct gggaagggcg atcgggaggc gcgggcactc tctagcaggt
      181 ggccgcagcc atggaggaga tctctgccgc cgctgtcaag gtcgtgccgg gcccggagcg
      241 cccgagccct ttcagccagc tagtctatac cagcaacgac tcctacatcg tccactctgg
      301 ggatcttaga aagatccata aagctgcctc ccggggacaa gtccggaagc tggagaagat
      361 gacaaagagg aagaagacca tcaaccttaa tatacaagac gcccagaaga ggactgctct
      421 acactgggcc tgtgtcaatg gccatgagga agtagtaaca tttctggtag acagaaagtg
      481 ccagcttgac gtccttgatg gcgaacacag gacacctctg atgaaggctc tacaatgcca
      541 tcaggaggct tgtgcaaata ttctgataga ttctggtgcc gatataaatc tcgtagatgt
      601 gtatggcaac acggctctcc attatgctgt ttatagtgag attttgtcag tggtggcaaa
      661 actgctgtcc catggtgcag tcatcgaagt gcacaacaag gctagcctca caccactttt
      721 actatccata acgaaaagaa gtgagcaaat tgtggaattt ttgctgataa aaaatgcaaa
      781 tgcgaatgca gttaataagt ataaatgcac agccctcatg cttgctgtat gtcatggatc
      841 atcagagata gttggcatgc ttcttcagca aaatgttgac gtctttgctg cagatatatg
      901 tggagtaact gcagaacatt atgctgttac ttgtggattt catcacattc atgaacaaat
      961 tatggaatat atacgaaaat tatctaaaaa tcatcaaaat accaatccag aaggaacatc
     1021 tgcaggaaca cctgatgagg ctgcaccctt ggcggaaaga acacctgaca cagctgaaag
     1081 cttggtggaa aaaacacctg atgaggctgc acccttggtg gaaagaacac ctgacacggc
     1141 tgaaagcttg gtggaaaaaa cacctgatga ggctgcatcc ttggtggagg gaacatctga
     1201 caaaattcaa tgtttggaga aagcgacatc tggaaagttc gaacagtcag cagaagaaac
     1261 acctagggaa attacgagtc ctgcaaaaga aacatctgag aaatttacgt ggccagcaaa
     1321 aggaagacct aggaagatcg catgggagaa aaaagaagac acacctaggg aaattatgag
     1381 tcccgcaaaa gaaacatctg agaaatttac gtgggcagca aaaggaagac ctaggaagat
     1441 cgcatgggag aaaaaagaaa cacctgtaaa gactggatgc gtggcaagag taacatctaa
     1501 taaaactaaa gttttggaaa aaggaagatc taagatgatt gcatgtccta caaaagaatc
     1561 atctacaaaa gcaagtgcca atgatcagag gttcccatca gaatccaaac aagaggaaga
     1621 tgaagaatat tcttgtgatt ctcggagtct ctttgagagt tctgcaaaga ttcaagtgtg
     1681 tatacctgag tctatatatc aaaaagtaat ggagataaat agagaagtag aagagcctcc
     1741 taagaagcca tctgccttca agcctgccat tgaaatgcaa aactctgttc caaataaagc
     1801 ctttgaattg aagaatgaac aaacattgag agcagatccg atgttcccac cagaatccaa
     1861 acaaaaggac tatgaagaaa attcttggga ttctgagagt ctctgtgaga ctgtttcaca
     1921 gaaggatgtg tgtttaccca aggctacaca tcaaaaagaa atagataaaa taaatggaaa
     1981 attagaagag tctcctaata aagatggtct tctgaaggct acctgcggaa tgaaagtttc
     2041 tattccaact aaagccttag aattgaagga catgcaaact ttcaaagcag agcctccggg
     2101 gaagccatct gccttcgagc ctgccactga aatgcaaaag tctgtcccaa ataaagcctt
     2161 ggaattgaaa aatgaacaaa cattgagagc agatgagata ctcccatcag aatccaaaca
     2221 aaaggactat gaagaaaatt cttgggatac tgagagtctc tgtgagactg tttcacagaa
     2281 ggatgtgtgt ttacccaagg ctgcgcatca aaaagaaata gataaaataa atggaaaatt
     2341 agaagggtct cctgttaaag atggtcttct gaaggctaac tgcggaatga aagtttctat
     2401 tccaactaaa gccttagaat tgatggacat gcaaactttc aaagcagagc ctcccgagaa
     2461 gccatctgcc ttcgagcctg ccattgaaat gcaaaagtct gttccaaata aagccttgga
     2521 attgaagaat gaacaaacat tgagagcaga tgagatactc ccatcagaat ccaaacaaaa
     2581 ggactatgaa gaaagttctt gggattctga gagtctctgt gagactgttt cacagaagga
     2641 tgtgtgttta cccaaggcta cgcatcaaaa agaaatagat aaaataaatg gaaaattaga
     2701 agagtctcct gataatgatg gttttctgaa ggctccctgc agaatgaaag tttctattcc
     2761 aactaaagcc ttagaattga tggacatgca aactttcaaa gcagagcctc ccgagaagcc
     2821 atctgccttc gagcctgcca ttgaaatgca aaagtctgtt ccaaataaag ccttggaatt
     2881 gaagaatgaa caaacattga gagcagatca gatgttccct tcagaatcaa aacaaaagaa
     2941 ggttgaagaa aattcttggg attctgagag tctccgtgag actgtttcac agaaggatgt
     3001 gtgtgtaccc aaggctacac atcaaaaaga aatggataaa ataagtggaa aattagaaga
     3061 ttcaactagc ctatcaaaaa tcttggatac agttcattct tgtgaaagag caagggaact
     3121 tcaaaaagat cactgtgaac aacgtacagg aaaaatggaa caaatgaaaa agaagttttg
     3181 tgtactgaaa aagaaactgt cagaagcaaa agaaataaaa tcacagttag agaaccaaaa
     3241 agttaaatgg gaacaagagc tctgcagtgt gagattgact ttaaaccaag aagaagagaa
     3301 gagaagaaat gccgatatat taaatgaaaa aattagggaa gaattaggaa gaatcgaaga
     3361 gcagcatagg aaagagttag aagtgaaaca acaacttgaa caggctctca gaatacaaga
     3421 tatagaattg aagagtgtag aaagtaattt gaatcaggtt tctcacactc atgaaaatga
     3481 aaattatctc ttacatgaaa attgcatgtt gaaaaaggaa attgccatgc taaaactgga
     3541 aatagccaca ctgaaacacc aataccagga aaaggaaaat aaatactttg aggacattaa
     3601 gattttaaaa gaaaagaatg ctgaacttca gatgacccta aaactgaaag aggaatcatt
     3661 aactaaaagg gcatctcaat atagtgggca gcttaaagtt ctgatagctg agaacacaat
     3721 gctcacttct aaattgaagg aaaaacaaga caaagaaata ctagaggcag aaattgaatc
     3781 acaccatcct agactggctt ctgctgtaca agaccatgat caaattgtga catcaagaaa
     3841 aagtcaagaa cctgctttcc acattgcagg agatgcttgt ttgcaaagaa aaatgaatgt
     3901 tgatgtgagt agtacgatat ataacaatga ggtgctccat caaccacttt ctgaagctca
     3961 aaggaaatcc aaaagcctaa aaattaatct caattatgca ggagatgctc taagagaaaa
     4021 tacattggtt tcagaacatg cacaaagaga ccaacgtgaa acacagtgtc aaatgaagga
     4081 agctgaacac atgtatcaaa acgaacaaga taatgtgaac aaacacactg aacagcagga
     4141 gtctctagat cagaaattat ttcaactaca aagcaaaaat atgtggcttc aacagcaatt
     4201 agttcatgca cataagaaag ctgacaacaa aagcaagata acaattgata ttcattttct
     4261 tgagaggaaa atgcaacatc atctcctaaa agagaaaaat gaggagatat ttaattacaa
     4321 taaccattta aaaaaccgta tatatcaata tgaaaaagag aaagcagaaa cagaaaactc
     4381 atgagagaca agcagtaaga aacttctttt ggagaaacaa cagaccagat ctttactcac
     4441 aactcatgct aggaggccag tcctagcatc accttatgtt gaaaatctta ccaatagtct
     4501 gtgtcaacag aatacttatt ttagaagaaa aattcatgat ttcttcctga agcctacaga
     4561 cataaaataa cagtgtgaag aattacttgt tcacgaattg cataaagctg cacaggattc
     4621 ccatctaccc tgatgatgca gcagacatca ttcaatccaa ccag
//
