LOCUS       NM_015114               4590 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens ankyrin repeat and LEM domain containing 2 (ANKLE2),
            mRNA.
ACCESSION   NM_015114 XM_925991
VERSION     NM_015114.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Thomas,A.X., Link,N., Robak,L.A., Demmler-Harrison,G., Pao,E.C.,
            Squire,A.E., Michels,S., Cohen,J.S., Comi,A., Prontera,P., Verrotti
            di Pianella,A., Di Cara,G., Garavelli,L., Caraffi,S.G., Fusco,C.,
            Zuntini,R., Parks,K.C., Sherr,E.H., Hashem,M.O., Maddirevula,S.,
            Alkuraya,F.S., Contractar,I.A.F., Neil,J.E., Walsh,C.A.,
            Bellen,H.J., Chao,H.T., Clark,R.D. and Mirzaa,G.M.
  TITLE     ANKLE2-related microcephaly: A variable microcephaly syndrome
            resembling Zika infection
  JOURNAL   Ann Clin Transl Neurol 9 (8), 1276-1288 (2022)
   PUBMED   35871307
  REMARK    GeneRIF: ANKLE2-related microcephaly: A variable microcephaly
            syndrome resembling Zika infection.
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Qin,Y., Huttlin,E.L., Winsnes,C.F., Gosztyla,M.L., Wacheul,L.,
            Kelly,M.R., Blue,S.M., Zheng,F., Chen,M., Schaffer,L.V., Licon,K.,
            Backstrom,A., Vaites,L.P., Lee,J.J., Ouyang,W., Liu,S.N., Zhang,T.,
            Silva,E., Park,J., Pitea,A., Kreisberg,J.F., Gygi,S.P., Ma,J.,
            Harper,J.W., Yeo,G.W., Lafontaine,D.L.J., Lundberg,E. and Ideker,T.
  TITLE     A multi-scale map of cell structure fusing protein images and
            interactions
  JOURNAL   Nature 600 (7889), 536-542 (2021)
   PUBMED   34819669
REFERENCE   3  (bases 1 to 4590)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 4590)
  AUTHORS   Link,N., Chung,H., Jolly,A., Withers,M., Tepe,B., Arenkiel,B.R.,
            Shah,P.S., Krogan,N.J., Aydin,H., Geckinli,B.B., Tos,T., Isikay,S.,
            Tuysuz,B., Mochida,G.H., Thomas,A.X., Clark,R.D., Mirzaa,G.M.,
            Lupski,J.R. and Bellen,H.J.
  TITLE     Mutations in ANKLE2, a ZIKA Virus Target, Disrupt an Asymmetric
            Cell Division Pathway in Drosophila Neuroblasts to Cause
            Microcephaly
  JOURNAL   Dev Cell 51 (6), 713-729 (2019)
   PUBMED   31735666
  REMARK    GeneRIF: that NS4A, ANKLE2, VRK1, and LLGL1 define a pathway
            impinging on asymmetric determinants of neural stem cell division
REFERENCE   5  (bases 1 to 4590)
  AUTHORS   Halfmann,C.T., Sears,R.M., Katiyar,A., Busselman,B.W., Aman,L.K.,
            Zhang,Q., O'Bryan,C.S., Angelini,T.E., Lele,T.P. and Roux,K.J.
  TITLE     Repair of nuclear ruptures requires barrier-to-autointegration
            factor
  JOURNAL   J Cell Biol 218 (7), 2136-2149 (2019)
   PUBMED   31147383
REFERENCE   6  (bases 1 to 4590)
  AUTHORS   Yamamoto,S., Jaiswal,M., Charng,W.L., Gambin,T., Karaca,E.,
            Mirzaa,G., Wiszniewski,W., Sandoval,H., Haelterman,N.A., Xiong,B.,
            Zhang,K., Bayat,V., David,G., Li,T., Chen,K., Gala,U., Harel,T.,
            Pehlivan,D., Penney,S., Vissers,L.E.L.M., de Ligt,J.,
            Jhangiani,S.N., Xie,Y., Tsang,S.H., Parman,Y., Sivaci,M.,
            Battaloglu,E., Muzny,D., Wan,Y.W., Liu,Z., Lin-Moore,A.T.,
            Clark,R.D., Curry,C.J., Link,N., Schulze,K.L., Boerwinkle,E.,
            Dobyns,W.B., Allikmets,R., Gibbs,R.A., Chen,R., Lupski,J.R.,
            Wangler,M.F. and Bellen,H.J.
  TITLE     A drosophila genetic resource of mutants to study mechanisms
            underlying human genetic diseases
  JOURNAL   Cell 159 (1), 200-214 (2014)
   PUBMED   25259927
REFERENCE   7  (bases 1 to 4590)
  AUTHORS   Asencio,C., Davidson,I.F., Santarella-Mellwig,R., Ly-Hartig,T.B.,
            Mall,M., Wallenfang,M.R., Mattaj,I.W. and Gorjanacz,M.
  TITLE     Coordination of kinase and phosphatase activities by Lem4 enables
            nuclear envelope reassembly during mitosis
  JOURNAL   Cell 150 (1), 122-135 (2012)
   PUBMED   22770216
  REMARK    GeneRIF: By coordinating VRK-1- and PP2A-mediated signaling on BAF,
            Lem4 and LEM-4L (Y55F3BR.8) controls postmitotic nuclear envelope
            formation in a function conserved from worms to humans.
REFERENCE   8  (bases 1 to 4590)
  AUTHORS   Ovsyannikova,I.G., Kennedy,R.B., O'Byrne,M., Jacobson,R.M.,
            Pankratz,V.S. and Poland,G.A.
  TITLE     Genome-wide association study of antibody response to smallpox
            vaccine
  JOURNAL   Vaccine 30 (28), 4182-4189 (2012)
   PUBMED   22542470
REFERENCE   9  (bases 1 to 4590)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   10 (bases 1 to 4590)
  AUTHORS   Beausoleil,S.A., Villen,J., Gerber,S.A., Rush,J. and Gygi,S.P.
  TITLE     A probability-based approach for high-throughput protein
            phosphorylation analysis and site localization
  JOURNAL   Nat Biotechnol 24 (10), 1285-1292 (2006)
   PUBMED   16964243
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB453975.1, DB451935.1,
            BC043157.1, AB014592.1, AK293028.1, BC062373.1, R59085.1 and
            AC135586.8.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_015114.2.
            
            Summary: This gene encodes a member of the LEM family of inner
            nuclear membrane proteins. The encoded protein functions as a
            mitotic regulator through postmitotic formation of the nuclear
            envelope. Mutations in this gene cause morphology defects in the
            nuclear envelope and BAF hyperphosphorylation. [provided by RefSeq,
            Mar 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.1179574.1,
                                           SRR18074967.2377414.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000357997.10/ ENSP00000350686.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-397               DB453975.1         57-453
            398-407             DB451935.1         431-440
            408-830             BC043157.1         410-832
            831-2192            AB014592.1         334-1695
            2193-3044           AK293028.1         573-1424
            3045-4422           BC062373.1         1109-2486
            4423-4426           R59085.1           18-21               c
            4427-4590           AC135586.8         23286-23449
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.33"
     gene            1..4590
                     /gene="ANKLE2"
                     /gene_synonym="KIAA0692; Lem4; LEMD7; MCPH16"
                     /note="ankyrin repeat and LEM domain containing 2"
                     /db_xref="GeneID:23141"
                     /db_xref="HGNC:HGNC:29101"
                     /db_xref="MIM:616062"
     exon            1..215
                     /gene="ANKLE2"
                     /gene_synonym="KIAA0692; Lem4; LEMD7; MCPH16"
                     /inference="alignment:Splign:2.1.0"
     CDS             35..2851
                     /gene="ANKLE2"
                     /gene_synonym="KIAA0692; Lem4; LEMD7; MCPH16"
                     /note="LEM domain containing 7; LEM domain-containing
                     protein 4; LEM-domain protein 4"
                     /codon_start=1
                     /product="ankyrin repeat and LEM domain-containing protein
                     2"
                     /protein_id="NP_055929.1"
                     /db_xref="CCDS:CCDS41869.1"
                     /db_xref="GeneID:23141"
                     /db_xref="HGNC:HGNC:29101"
                     /db_xref="MIM:616062"
                     /translation="MLWPRLAAAEWAALAWELLGASVLLIAVRWLVRRLGPRPGGLGR
                     SGTPVPPPSAAAAPASGEMTMDALLARLKLLNPDDLREEIVKAGLKCGPITSTTRFIF
                     EKKLAQALLEQGGRLSSFYHHEAGVTALSQDPQRILKPAEGNPTDQAGFSEDRDFGYS
                     VGLNPPEEEAVTSKTCSVPPSDTDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYV
                     YENKKEALQAVKMIKGSRFKAFSTREDAEKFARGICDYFPSPSKTSLPLSPVKTAPLF
                     SNDRLKDGLCLSESETVNKERANSYKNPRTQDLTAKLRKAVEKGEEDTFSDLIWSNPR
                     YLIGSGDNPTIVQEGCRYNVMHVAAKENQASICQLTLDVLENPDFMRLMYPDDDEAML
                     QKRIRYVVDLYLNTPDKMGYDTPLHFACKFGNADVVNVLSSHHLIVKNSRNKYDKTPE
                     DVICERSKNKSVELKERIREYLKGHYYVPLLRAEETSSPVIGELWSPDQTAEASHVSR
                     YGGSPRDPVLTLRAFAGPLSPAKAEDFRKLWKTPPREKAGFLHHVKKSDPERGFERVG
                     RELAHELGYPWVEYWEFLGCFVDLSSQEGLQRLEEYLTQQEIGKKAQQETGEREASCR
                     DKATTSGSNSISVRAFLDEDDMSLEEIKNRQNAARNNSPPTVGAFGHTRCSAFPLEQE
                     ADLIEAAEPGGPHSSRNGLCHPLNHSRTLAGKRPKAPRGEEAHLPPVSDLTVEFDKLN
                     LQNIGRSVSKTPDESTKTKDQILTSRINAVERDLLEPSPADQLGNGHRRTESEMSARI
                     AKMSLSPSSPRHEDQLEVTREPARRLFLFGEEPSKLDQDVLAALECADVDPHQFPAVH
                     RWKSAVLCYSPSDRQSWPSPAVKGRFKSQLPDLSGPHSYSPGRNSVAGSNPAKPGLGS
                     PGRYSPVHGSQLRRMARLAELAAL"
     exon            216..674
                     /gene="ANKLE2"
                     /gene_synonym="KIAA0692; Lem4; LEMD7; MCPH16"
                     /inference="alignment:Splign:2.1.0"
     exon            675..881
                     /gene="ANKLE2"
                     /gene_synonym="KIAA0692; Lem4; LEMD7; MCPH16"
                     /inference="alignment:Splign:2.1.0"
     exon            882..1075
                     /gene="ANKLE2"
                     /gene_synonym="KIAA0692; Lem4; LEMD7; MCPH16"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1264
                     /gene="ANKLE2"
                     /gene_synonym="KIAA0692; Lem4; LEMD7; MCPH16"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1387
                     /gene="ANKLE2"
                     /gene_synonym="KIAA0692; Lem4; LEMD7; MCPH16"
                     /inference="alignment:Splign:2.1.0"
     exon            1388..1454
                     /gene="ANKLE2"
                     /gene_synonym="KIAA0692; Lem4; LEMD7; MCPH16"
                     /inference="alignment:Splign:2.1.0"
     exon            1455..1627
                     /gene="ANKLE2"
                     /gene_synonym="KIAA0692; Lem4; LEMD7; MCPH16"
                     /inference="alignment:Splign:2.1.0"
     exon            1628..1734
                     /gene="ANKLE2"
                     /gene_synonym="KIAA0692; Lem4; LEMD7; MCPH16"
                     /inference="alignment:Splign:2.1.0"
     exon            1735..1925
                     /gene="ANKLE2"
                     /gene_synonym="KIAA0692; Lem4; LEMD7; MCPH16"
                     /inference="alignment:Splign:2.1.0"
     exon            1926..2517
                     /gene="ANKLE2"
                     /gene_synonym="KIAA0692; Lem4; LEMD7; MCPH16"
                     /inference="alignment:Splign:2.1.0"
     exon            2518..2649
                     /gene="ANKLE2"
                     /gene_synonym="KIAA0692; Lem4; LEMD7; MCPH16"
                     /inference="alignment:Splign:2.1.0"
     exon            2650..4590
                     /gene="ANKLE2"
                     /gene_synonym="KIAA0692; Lem4; LEMD7; MCPH16"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2943..2948
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANKLE2"
                     /gene_synonym="KIAA0692; Lem4; LEMD7; MCPH16"
                     /note="hexamer: ATTAAA"
     polyA_site      2961
                     /gene="ANKLE2"
                     /gene_synonym="KIAA0692; Lem4; LEMD7; MCPH16"
     regulatory      4405..4410
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANKLE2"
                     /gene_synonym="KIAA0692; Lem4; LEMD7; MCPH16"
                     /note="hexamer: AGTAAA"
     polyA_site      4425
                     /gene="ANKLE2"
                     /gene_synonym="KIAA0692; Lem4; LEMD7; MCPH16"
                     /note="major polyA site"
     regulatory      4574..4579
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANKLE2"
                     /gene_synonym="KIAA0692; Lem4; LEMD7; MCPH16"
                     /note="hexamer: AATGAA"
     polyA_site      4590
                     /gene="ANKLE2"
                     /gene_synonym="KIAA0692; Lem4; LEMD7; MCPH16"
ORIGIN      
        1 gggcttctcg gcggctgcgg cccgggcggc ggcgatgctg tggccgcggc tggcggcggc
       61 cgagtgggcg gcgctggcct gggagctgct gggcgcctcg gtgctgctga tcgctgtgcg
      121 gtggctggtg cggcggctgg gcccgcggcc gggaggtctg ggccgcagcg ggaccccagt
      181 tcctccgcca agcgcggccg ccgcccccgc ctcaggtgaa atgacaatgg atgctctgtt
      241 ggctcgattg aaacttctga atccagatga ccttagagaa gaaatcgtca aagccggatt
      301 gaaatgtgga cccattacat caactacaag gttcattttt gagaaaaaat tggctcaggc
      361 tttactggag caaggaggaa ggctgtcttc tttctaccac catgaggcag gtgtcacagc
      421 tctcagccag gacccacaaa ggattttgaa gccagctgaa gggaacccaa ctgatcaggc
      481 tggtttttct gaagacagag attttggtta cagtgtgggc ctgaatcctc cagaggagga
      541 agctgtgaca tccaagacct gctcggtgcc ccctagtgac accgacacct acagagctgg
      601 agcgactgcg tctaaggagc cgcccctgta ctatggggtg tgtccagtgt atgaggacgt
      661 cccagcgaga aatgaaagga tctatgttta tgaaaataaa aaggaagcat tgcaagctgt
      721 caagatgatc aaagggtccc gatttaaagc tttttctacc agagaagacg ctgagaaatt
      781 tgctagagga atttgtgatt atttcccttc tccaagcaaa acgtccttac cactgtctcc
      841 tgtgaaaaca gctccactct ttagcaatga caggttgaaa gatggtttgt gcttgtcgga
      901 atcagaaaca gtcaacaaag agcgagcgaa cagttacaaa aatccccgca cgcaggacct
      961 caccgccaag cttcggaaag ctgtggagaa gggagaggag gacacctttt ctgaccttat
     1021 ctggagcaac ccccggtatc tgataggctc aggagacaac cccactatcg tgcaggaagg
     1081 gtgcaggtac aacgtgatgc atgttgctgc caaagagaac caggcttcca tctgccagct
     1141 gactctggac gtcctggaga accctgactt catgaggctg atgtaccctg atgacgacga
     1201 ggccatgctg cagaagcgta tccgttacgt ggtggacctg tacctcaaca cccccgacaa
     1261 gatgggctat gacacaccgt tgcattttgc ttgtaagttt ggaaatgcag atgtagtcaa
     1321 cgtgctttcg tcacaccatt tgattgtaaa aaactcaagg aataaatatg ataaaacacc
     1381 tgaagatgta atttgtgaaa gaagcaaaaa taaatctgtg gaactgaagg agcggatcag
     1441 agagtattta aagggccact actacgtgcc cctcctgaga gcggaagaga cttcttctcc
     1501 agtcatcggg gagctgtggt ccccagacca gacggctgag gcctctcacg tcagccgcta
     1561 tggaggcagc cccagagacc cggtactgac cctgagagcc ttcgcagggc ccctgagtcc
     1621 agccaaggca gaagattttc gcaagctctg gaaaactcca cctcgagaga aagcaggctt
     1681 ccttcaccac gtcaagaagt cggacccgga aagaggcttt gagagagtgg gaagggagct
     1741 agctcatgag ctggggtatc cctgggttga atactgggaa tttctgggct gttttgttga
     1801 tctgtcttcc caggaaggcc tgcaaagact agaagaatat ctcacacagc aggaaatagg
     1861 caaaaaggct caacaagaaa caggagaacg ggaagcctcc tgccgagata aagccaccac
     1921 gtctggcagc aattccattt ccgtgagggc gtttctagat gaagatgaca tgagcttgga
     1981 agaaataaaa aatcggcaaa atgcagctcg aaataacagc ccgcccacag tcggtgcttt
     2041 tggacatacg aggtgcagcg ccttcccctt ggagcaggag gcagacctca tagaagccgc
     2101 cgagccggga ggtccacaca gcagcagaaa tgggctctgc catcctctga atcacagcag
     2161 gaccctggcg ggcaagagac caaaggcccc ccgtggggag gaagcccatc tgccacctgt
     2221 ctcggatttg actgttgagt ttgataaact gaatttgcaa aatataggac gtagcgtttc
     2281 caagacacca gatgaaagta caaaaactaa agatcagatc ctgacttcaa gaatcaatgc
     2341 agtagaaaga gacttgttag agccttctcc cgcagaccaa ctcgggaatg gccacaggag
     2401 gacagaaagt gaaatgtcag ccaggatcgc taaaatgtcc ttgagtccca gcagccccag
     2461 gcacgaggat cagctcgagg tcaccaggga accggccagg cggctcttcc tttttggaga
     2521 ggagccatca aaactcgatc aggatgtttt ggccgctctt gaatgtgcag acgtcgaccc
     2581 ccatcagttc ccggccgtgc acagatggaa gagtgctgtc ctgtgctact caccctcgga
     2641 cagacagagt tggcccagtc ccgcggtgaa aggaaggttc aagtctcagc tgccagatct
     2701 cagtggccct cacagctaca gtccggggag aaacagcgtg gctggaagca accccgcaaa
     2761 gccaggcctg ggcagtcctg ggcgctacag ccccgtgcac gggagccagc tccgcaggat
     2821 ggcgcgcctg gctgagcttg ccgccctgta ggcttggcgc tgggctctcg gtttgttctt
     2881 catttttaaa gaaggaaggg tcatatgttt attgctaaac tgtcaaaaag gaatatattc
     2941 tgattaaatt attactcctc actttgaggg tgtgagaatt ttagaagatt taaatgttct
     3001 atataacact tagatttctg atattttgga agaagttaga agttaatgaa agcaaactca
     3061 gttaccaatt ttctggaaaa tatccatgtg gtaatggtag actttttagg tggcaatttc
     3121 taggtctgaa atatagcaga ggaaagggcg ctgaggcagt tgcaggcagg cagccctgta
     3181 cttaccctgt actcacctca tccgacagac gctgtggatg aggaggggct tggcggaggc
     3241 gtgagcaccg atgtcccttt gataacctgc actcaccaag atgaactatt tgccgccctg
     3301 tcttttcctg ggttgggggg tggcatctga tggtggcaga gtgcctgttg gttcgcccgt
     3361 gggtctcatg gttcagacag agggaggtgg acggcaggga tcagggagcc aggagcgcgc
     3421 ctcagacttg cagcaaccat tgtgatttgg gttgttcgga atatttaaat tactgatcag
     3481 aagatgaaag tagcttttct cttgggaagt cttgcagccc gtgggagtga taccaggagc
     3541 aacacagagc tcagcagcgg cgccaaggtg ttccctgttt cctcagcacg tgagccttca
     3601 ccgcctgctt cattcaggag ccagtgcagc agtaatacag tctatacatt gttctgtttt
     3661 caaatttatc ctgaggcttt gttgagcata aatgattata cgataaaggt atccgttatt
     3721 ttggaactca tttcagttgg gatctcctgt atgcagagtg ttgcatttag aggtttgagt
     3781 cccatcttgg tttcttgccg tgctgactgt agccttcacc ttgacttgaa tgaaggtctg
     3841 tggttggaat gtgtgaggag ccgctgaggt gttcaggagg tgctgcctgg aggtcggttt
     3901 cttcctgggt gttacgggca actgctcaca cagttgtttc tctgtgaaca tttccagtgt
     3961 ttaatccaaa atgaaaaccc accaatgctt ttgctaactt cagtgccttt tataaatcat
     4021 ttttaaattt cctgaacttg ctttttgagg atatacaggg atattaagta gacgcaggat
     4081 tgtttttgtt tgtaaaaatt ctgaattgaa actttgtttt aaaaaaaggc ttctttcttt
     4141 catatgacaa gagataggtc aggaatattg gaatcaagat ttaaatgtta aaattcgatt
     4201 ttgttacaca gggtgtgttc atttgttttg tagcagacaa gatctagatc ccagacagaa
     4261 acaacacatg ctattctaaa aagccgcatt ttaaaaggca ccttggttct caaaagaaat
     4321 cagaatatgg atattcgtag tgatgatctg ttttctctaa aatcttacca tattgtctgt
     4381 atatggttgt aaattcaaat ggaaagtaaa acgttttggc cctgattttg tatgtggacc
     4441 actgctcctg atttcccagg tcttaggcca cctttgactg tttctccgtt tgtttgtggg
     4501 cagcgattcc agtcccaacg gaggcattct cgtgtgtccc ggggggttat gtccttcaca
     4561 aaacacttaa tgaaatgaat tacttcaaaa
//
