LOCUS       NM_017747               8195 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1),
            transcript variant 1, mRNA.
ACCESSION   NM_017747
VERSION     NM_017747.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Mullenger,J.L., Zeidler,M.P. and Fragiadaki,M.
  TITLE     Evaluating the Molecular Properties and Function of ANKHD1, and Its
            Role in Cancer
  JOURNAL   Int J Mol Sci 24 (16), 12834 (2023)
   PUBMED   37629022
  REMARK    GeneRIF: Evaluating the Molecular Properties and Function of
            ANKHD1, and Its Role in Cancer.
            Review article
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8195)
  AUTHORS   Yao,P.A., Wu,Y., Zhao,K., Li,Y., Cao,J. and Xing,C.
  TITLE     The feedback loop of ANKHD1/lncRNA MALAT1/YAP1 strengthens the
            radioresistance of CRC by activating YAP1/AKT signaling
  JOURNAL   Cell Death Dis 13 (2), 103 (2022)
   PUBMED   35110552
  REMARK    GeneRIF: The feedback loop of ANKHD1/lncRNA MALAT1/YAP1 strengthens
            the radioresistance of CRC by activating YAP1/AKT signaling.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8195)
  AUTHORS   Dhyani,A., Favaro,P. and Olalla Saad,S.T.
  TITLE     ANKHD1 is an S phase protein required for histone synthesis and DNA
            repair in multiple myeloma cells
  JOURNAL   Blood Cells Mol Dis 84, 102460 (2020)
   PUBMED   32562952
  REMARK    GeneRIF: ANKHD1 is an S phase protein required for histone
            synthesis and DNA repair in multiple myeloma cells.
REFERENCE   4  (bases 1 to 8195)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 8195)
  AUTHORS   Almeida,B.O. and Machado-Neto,J.A.
  TITLE     Emerging functions for ANKHD1 in cancer-related signaling pathways
            and cellular processes
  JOURNAL   BMB Rep 53 (8), 413-418 (2020)
   PUBMED   32635985
  REMARK    GeneRIF: Emerging functions for ANKHD1 in cancer-related signaling
            pathways and cellular processes.
            Review article
REFERENCE   6  (bases 1 to 8195)
  AUTHORS   Wang,J., Huo,K., Ma,L., Tang,L., Li,D., Huang,X., Yuan,Y., Li,C.,
            Wang,W., Guan,W., Chen,H., Jin,C., Wei,J., Zhang,W., Yang,Y.,
            Liu,Q., Zhou,Y., Zhang,C., Wu,Z., Xu,W., Zhang,Y., Liu,T., Yu,D.,
            Zhang,Y., Chen,L., Zhu,D., Zhong,X., Kang,L., Gan,X., Yu,X., Ma,Q.,
            Yan,J., Zhou,L., Liu,Z., Zhu,Y., Zhou,T., He,F. and Yang,X.
  TITLE     Toward an understanding of the protein interaction network of the
            human liver
  JOURNAL   Mol Syst Biol 7, 536 (2011)
   PUBMED   21988832
  REMARK    Erratum:[Mol Syst Biol. 2017 Dec 18;13(12):965. doi:
            10.15252/msb.20178107.. PMID: 29254952]
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 8195)
  AUTHORS   Traina,F., Favaro,P.M., Medina Sde,S., Duarte Ada,S.,
            Winnischofer,S.M., Costa,F.F. and Saad,S.T.
  TITLE     ANKHD1, ankyrin repeat and KH domain containing 1, is overexpressed
            in acute leukemias and is associated with SHP2 in K562 cells
  JOURNAL   Biochim Biophys Acta 1762 (9), 828-834 (2006)
   PUBMED   16956752
  REMARK    GeneRIF: These findings suggest a role for ANKHD1 as a scaffolding
            protein that may be associated with the abnormal phenotype of
            leukemia cells.
REFERENCE   8  (bases 1 to 8195)
  AUTHORS   Santos Duarte Ada,S., Traina,F., Favaro,P.M., Basseres,D.S., de
            Carvalho,I.C., Medina Sde,S., Costa,F.F. and Saad,S.T.
  TITLE     Characterisation of a new splice variant of MASK-BP3(ARF) and MASK
            human genes, and their expression patterns during haematopoietic
            cell differentiation
  JOURNAL   Gene 363, 113-122 (2005)
   PUBMED   16297570
  REMARK    GeneRIF: identification of new splice variant with upregulation of
            its mRNA considerably higher than other variants during erythroid
            differentiation
REFERENCE   9  (bases 1 to 8195)
  AUTHORS   Miles,M.C., Janket,M.L., Wheeler,E.D., Chattopadhyay,A.,
            Majumder,B., Dericco,J., Schafer,E.A. and Ayyavoo,V.
  TITLE     Molecular and functional characterization of a novel splice variant
            of ANKHD1 that lacks the KH domain and its role in cell survival
            and apoptosis
  JOURNAL   FEBS J 272 (16), 4091-4102 (2005)
   PUBMED   16098192
  REMARK    GeneRIF: VBARP is a novel splice variant of ANKHD1 and may play a
            role in cellular apoptosis (antiapoptotic) and cell survival
            pathway(s
REFERENCE   10 (bases 1 to 8195)
  AUTHORS   Poulin,F., Brueschke,A. and Sonenberg,N.
  TITLE     Gene fusion and overlapping reading frames in the mammalian genes
            for 4E-BP3 and MASK
  JOURNAL   J Biol Chem 278 (52), 52290-52297 (2003)
   PUBMED   14557257
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA509209.1, AF521882.1 and
            AK000904.1.
            
            On Nov 22, 2018 this sequence version replaced NM_017747.2.
            
            Summary: This gene encodes a protein with multiple ankyrin repeat
            domains and a single KH-domain. The protein is thought to function
            as a scaffolding protein, and it may be involved in the regulation
            of caspases and thereby play an antiapoptotic role in cell
            survival. Alternative splicing results in multiple transcript
            variants, one of which generates a fusion transcript (MASK-BP3)
            with the downstream eIF4E-binding protein 3 (EIF4EBP3) gene,
            resulting in a protein comprised of the ANKHD1 sequence for the
            majority of the protein and a different C-terminus due to an
            alternate reading frame for the EIF4EBP3 segments. [provided by
            RefSeq, Sep 2010].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and it encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF521882.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000360839.7/ ENSP00000354085.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-75                DA509209.1         20-94
            76-3008             AF521882.1         1-2933
            3009-3701           AK000904.1         868-1560
            3702-8195           AF521882.1         3627-8120
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q31.3"
     gene            1..8195
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /note="ankyrin repeat and KH domain containing 1"
                     /db_xref="GeneID:54882"
                     /db_xref="HGNC:HGNC:24714"
                     /db_xref="MIM:610500"
     exon            1..441
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     CDS             136..7764
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /note="isoform 1 is encoded by transcript variant 1;
                     multiple ankyrin repeats, single KH-domain homolog; HIV-1
                     Vpr-binding ankyrin repeat protein"
                     /codon_start=1
                     /product="ankyrin repeat and KH domain-containing protein
                     1 isoform 1"
                     /protein_id="NP_060217.1"
                     /db_xref="CCDS:CCDS4225.1"
                     /db_xref="GeneID:54882"
                     /db_xref="HGNC:HGNC:24714"
                     /db_xref="MIM:610500"
                     /translation="MLTDSGGGGTSFEEDLDSVAPRSAPAGASEPPPPGGVGLGIRTV
                     RLFGEAGPASGVGSSGGGGSGSGTGGGDAALDFKLAAAVLRTGGGGGASGSDEDEVSE
                     VESFILDQEDLDNPVLKTTSEIFLSSTAEGADLRTVDPETQARLEALLEAAGIGKLST
                     ADGKAFADPEVLRRLTSSVSCALDEAAAALTRMKAENSHNAGQVDTRSLAEACSDGDV
                     NAVRKLLDEGRSVNEHTEEGESLLCLACSAGYYELAQVLLAMHANVEDRGNKGDITPL
                     MAASSGGYLDIVKLLLLHDADVNSQSATGNTALTYACAGGFVDIVKVLLNEGANIEDH
                     NENGHTPLMEAASAGHVEVARVLLDHGAGINTHSNEFKESALTLACYKGHLDMVRFLL
                     EAGADQEHKTDEMHTALMEACMDGHVEVARLLLDSGAQVNMPADSFESPLTLAACGGH
                     VELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLLAQGANINAQTEETQETA
                     LTLACCGGFSEVADFLIKAGADIELGCSTPLMEASQEGHLELVKYLLASGANVHATTA
                     TGDTALTYACENGHTDVADVLLQAGADLEHESEGGRTPLMKAARAGHLCTVQFLISKG
                     ANVNRATANNDHTVVSLACAGGHLAVVELLLAHGADPTHRLKDGSTMLIEAAKGGHTN
                     VVSYLLDYPNNVLSVPTTDVSQLPPPSQDQSQVPRVPTHTLAMVVPPQEPDRTSQENS
                     PALLGVQKGTSKQKSSSLQVADQDLLPSFHPYQPLECIVEETEGKLNELGQRISAIEK
                     AQLKSLELIQGEPLNKDKIEELKKNREEQVQKKKKILKELQKVERQLQMKTQQQFTKE
                     YLETKGQKDTVSLHQQCSHRGVFPEGEGDGSLPEDHFSELPQVDTILFKDNDVDDEQQ
                     SPPSAEQIDFVPVQPLSSPQCNFSSDLGSNGTNSLELQKVSGNQQIVGQPQIAITGHD
                     QGLLVQEPDGLMVATPAQTLTDTLDDLIAAVSTRVPTGSNSSSQTTECLTPESCSQTT
                     SNVASQSMPPVYPSVDIDAHTESNHDTALTLACAGGHEELVSVLIARDAKIEHRDKKG
                     FTPLILAATAGHVGVVEILLDKGGDIEAQSERTKDTPLSLACSGGRQEVVDLLLARGA
                     NKEHRNVSDYTPLSLAASGGYVNIIKILLNAGAEINSRTGSKLGISPLMLAAMNGHVP
                     AVKLLLDMGSDINAQIETNRNTALTLACFQGRAEVVSLLLDRKANVEHRAKTGLTPLM
                     EAASGGYAEVGRVLLDKGADVNAPPVPSSRDTALTIAADKGHYKFCELLIHRGAHIDV
                     RNKKGNTPLWLASNGGHFDVVQLLVQAGADVDAADNRKITPLMSAFRKGHVKVVQYLV
                     KEVNQFPSDIECMRYIATITDKELLKKCHQCVETIVKAKDQQAAEANKNASILLKELD
                     LEKSREESRKQALAAKREKRKEKRKKKKEEQKRKQEEDEENKPKENSELPEDEDEEEN
                     DEDVEQEVPIEPPSATTTTTIGISATSATFTNVFGKKRANVVTTPSTNRKNKKNKTKE
                     TPPTAHLILPEQHMSLAQQKADKNKINGEPRGGGAGGNSDSDNLDSTDCNSESSSGGK
                     SQELNFVMDVNSSKYPSLLLHSQEEKTSTATSKTQTRLEGEVTPNSLSTSYKTVSLPL
                     SSPNIKLNLTSPKRGQKREEGWKEVVRRSKKLSVPASVVSRIMGRGGCNITAIQDVTG
                     AHIDVDKQKDKNGERMITIRGGTESTRYAVQLINALIQDPAKELEDLIPKNHIRTPAS
                     TKSIHANFSSGVGTTAASSKNAFPLGAPTLVTSQATTLSTFQPANKLNKNVPTNVRSS
                     FPVSLPLAYPHPHFALLAAQTMQQIRHPRLPMAQFGGTFSPSPNTWGPFPVRPVNPGN
                     TNSSPKHNNTSRLPNQNGTVLPSESAGLATASCPITVSSVVAASQQLCVTNTRTPSSV
                     RKQLFACVPKTSPPATVISSVTSTCSSLPSVSSAPITSGQAPTTFLPASTSQAQLSSQ
                     KMESFSAVPPTKEKVSTQDQPMANLCTPSSTANSCSSSASNTPGAPETHPSSSPTPTS
                     SNTQEEAQPSSVSDLSPMSMPFASNSEPAPLTLTSPRMVAADNQDTSNLPQLAVPAPR
                     VSHRMQPRGSFYSMVPNATIHQDPQSIFVTNPVTLTPPQGPPAAVQLSSAVNIMNGSQ
                     MHINPANKSLPPTFGPATLFNHFSSLFDSSQVPANQGWGDGPLSSRVATDASFTVQSA
                     FLGNSVLGHLENMHPDNSKAPGFRPPSQRVSTSPVGLPSIDPSGSSPSSSSAPLASFS
                     GIPGTRVFLQGPAPVGTPSFNRQHFSPHPWTSASNSSTSAPPTLGQPKGVSASQDRKI
                     PPPIGTERLARIRQGGSVAQAPAGTSFVAPVGHSGIWSFGVNAVSEGLSGWSQSVMGN
                     HPMHQQLSDPSTFSQHQPMERDDSGMVAPSNIFHQPMASGFVDFSKGLPISMYGGTII
                     PSHPQLADVPGGPLFNGLHNPDPAWNPMIKVIQNSTECTDAQQIWPGTWAPHIGNMHL
                     KYVN"
     exon            442..595
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            596..752
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            753..900
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1048
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1282
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1377
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..1615
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            1616..1807
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            1808..1917
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            1918..2005
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            2006..2133
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            2134..2277
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            2278..2380
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            2381..3139
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            3140..3285
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            3286..3397
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            3398..3543
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            3544..3686
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            3687..3900
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            3901..4076
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            4077..4239
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            4240..4335
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            4336..4452
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            4453..4627
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            4628..5075
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            5076..5222
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            5223..5372
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            5373..6985
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            6986..7162
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            7163..7363
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            7364..7539
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            7540..7704
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     exon            7705..8195
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8177..8182
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /note="hexamer: AATAAA"
     polyA_site      8195
                     /gene="ANKHD1"
                     /gene_synonym="MASK; MASK1; PP2500; VBARP"
                     /note="major polyA site"
ORIGIN      
        1 agtgagaagt tagtggcgct gctgggacgg gggaaaggag acgcttcttc ctcttgctgc
       61 tcttctcgtt cccgagatca gcggcggcgg tgaccgcgag tgggtcggca ccgtctccgg
      121 ctccgggtgc gaacaatgct gactgatagc ggaggcggcg gcacctcctt tgaggaggac
      181 ctggactctg tggctccgcg atccgcccca gctggggcct cggagccgcc tccgccggga
      241 ggggtcggtc tggggatccg caccgtgagg ctctttgggg aggccgggcc agcgtcggga
      301 gtcggcagca gcggcggcgg cggcagcggc agcggtacgg gcggagggga cgcggcgctg
      361 gatttcaagt tggcggctgc cgtgctgagg accgggggtg gaggtggtgc ctctggcagt
      421 gacgaggacg aagtgtccga ggttgaatca tttattttgg accaagaaga tctggataac
      481 ccagtgctta aaacaacatc agagatattc ttatcaagta ctgcagaagg agcagactta
      541 cgcactgtgg atccagagac acaggcacga ctagaagcat tgctagaagc agcaggaatt
      601 ggcaaattgt caactgctga tggtaaagct tttgcagatc ctgaggtact ccggagactg
      661 acatcctcag ttagttgtgc actggatgaa gctgctgctg cactgacacg gatgaaagca
      721 gaaaacagcc acaatgcagg acaagtggac actcgcagtc tagcagaagc ttgttcagat
      781 ggggatgtta atgctgttcg taaattgcta gatgaaggca gaagtgtaaa tgaacataca
      841 gaagaaggag aaagcctgct gtgtttggct tgttcagcag ggtattatga attagcacaa
      901 gtattgcttg ctatgcatgc taatgttgaa gatcgaggga ataaaggaga cataactccc
      961 ctgatggcag cttccagtgg aggttactta gatattgtga aattattact tcttcatgat
     1021 gctgatgtca actcccagtc tgcaacagga aacactgcgc taacttatgc atgtgctgga
     1081 ggatttgttg acattgttaa agtgctcctt aatgaaggtg caaatataga agatcataat
     1141 gaaaatggac atactccctt aatggaagca gccagtgcag gtcatgtgga agttgcaaga
     1201 gttcttttag atcatggtgc aggcatcaac actcattcta atgaattcaa agaaagtgct
     1261 ctaacacttg cttgctacaa aggccatttg gatatggttc gctttctact tgaagctggt
     1321 gcagatcaag agcacaaaac agatgagatg cacactgcct taatggaggc ctgcatggat
     1381 ggacatgtag aggtggcacg tttgcttttg gatagtggtg ctcaagtgaa catgcctgca
     1441 gattcatttg aatctccatt gacgctagct gcctgtggag gacatgttga attggcagct
     1501 ctacttattg aaaggggagc aaatcttgaa gaagttaatg atgaaggata cactcccttg
     1561 atggaagctg cccgggaagg acatgaagaa atggtggcac tactcttagc acaaggagca
     1621 aatataaatg cccagacaga agaaactcaa gaaactgctc ttactttggc ttgctgtgga
     1681 ggattttctg aagttgcaga ctttcttatt aaggcagggg ctgatataga acttggctgc
     1741 tccacacctc tgatggaggc atctcaggag ggacacctgg aattggttaa atatttgctg
     1801 gcttctggcg ctaatgtgca tgctacaaca gcaacaggag acacagcctt aacctatgct
     1861 tgtgaaaatg gacatacgga tgttgcagat gttttacttc aagcaggggc tgatttagaa
     1921 catgaatctg aaggtggaag aacacctttg atgaaagctg caagagctgg tcatttgtgc
     1981 actgtgcagt ttcttattag caaaggtgcc aatgttaaca gggctacagc caataatgat
     2041 catacagtag tgtcgctggc atgtgcagga ggccacctgg cagttgttga gcttctcttg
     2101 gctcatgggg ctgaccctac tcatcgactc aaggatggtt caacaatgct cattgaagct
     2161 gcaaagggtg gccatactaa tgtagtttct tatctgttgg attatccaaa taatgttctg
     2221 tcagttccca ccacagatgt gtctcagctc cctccacctt ctcaagatca gtctcaggtg
     2281 ccacgtgtgc caacgcatac acttgccatg gttgtacctc cccaggaacc tgacagaact
     2341 tcacaggaga actctcctgc ccttttagga gtgcaaaaag gtacatccaa gcagaagtcc
     2401 agttccctcc aggtagcaga tcaggaccta ctgccatctt ttcacccata ccagcctttg
     2461 gagtgcatag tagaggagac tgaaggcaag ctgaatgaac tgggacaaag aattagtgct
     2521 attgaaaaag cacagcttaa gtcactggag ttaattcaag gtgaacctct gaacaaagat
     2581 aagatagaag aacttaaaaa gaacagagaa gagcaagtcc agaagaagaa gaaaatattg
     2641 aaagaactgc agaaagtgga aaggcagttg cagatgaaaa cacagcagca atttaccaaa
     2701 gaatacttgg aaaccaaagg tcagaaagac acagtgtctc tacaccaaca gtgctctcat
     2761 agaggagtct tcccagaagg ggaaggagat ggtagtctcc cagaggatca cttttcagag
     2821 ttacctcagg ttgacacaat cttatttaaa gataatgatg ttgatgatga gcaacagtct
     2881 ccaccatcgg cagaacagat tgattttgtc ccagtccagc ctttatcatc tccacagtgt
     2941 aacttttcca gtgacttagg ttctaatggg acaaattctc ttgaacttca gaaagtatca
     3001 ggtaatcagc agattgtagg acagcctcag attgctatta ctggacatga tcaggggctg
     3061 ttagttcaag aaccagatgg actaatggtt gcaactccag ctcagacgct taccgacact
     3121 cttgatgacc tgatagcagc tgtgagtacc agagtgccca ctggttccaa cagttcttct
     3181 cagaccacag agtgtcttac acctgaatcc tgttcgcaga ctacaagcaa tgtggcttcc
     3241 caatcgatgc ctcctgtgta tccttcagtt gacattgatg cacatactga gagcaatcat
     3301 gacacagcat taacactagc ttgtgcaggt ggtcatgaag aacttgtatc tgtgctcatt
     3361 gcacgggatg ccaaaattga acacagagac aaaaaaggtt tcacaccact aatcctggca
     3421 gcaacagcag ggcatgttgg agttgttgaa atccttttgg ataaaggtgg agatatagaa
     3481 gcacagtctg aacgaactaa ggatactccg ctttcattgg catgttctgg tggacgtcag
     3541 gaggtggtag acttgctgct ggctcgaggt gcaaataaag aacataggaa cgtatctgat
     3601 tatacaccac tgagtctagc tgcgtctgga ggatatgtta atatcattaa gattctgctt
     3661 aatgctgggg cagaaattaa ttcaaggact gggagtaaac taggtatttc tcccctgatg
     3721 ttggctgcaa tgaatggaca tgttcctgca gtaaaattgc tgctcgatat gggttcagac
     3781 attaatgccc aaatagagac caatcggaac acggctctca ccctggcctg tttccagggc
     3841 cgagcagaag tagtgagttt gcttctggac cgaaaagcca atgttgaaca tagggcaaag
     3901 acgggtctta cccccttgat ggaagcagct tctggagggt atgcagaggt tggaagagtt
     3961 cttcttgata aaggagcaga tgttaatgct ccccctgtgc cttcctcaag agatactgct
     4021 ttaacaatag cagcagacaa aggtcactac aaattttgtg aactcctgat tcatagggga
     4081 gcccacattg atgttcgtaa caaaaaggga aatacgccac tttggctggc atccaatgga
     4141 ggtcattttg atgttgtgca gttgctagtg caagcaggtg ctgatgtgga tgcagcagat
     4201 aaccggaaaa tcacacctct tatgtcagca tttcgcaagg gtcatgtaaa agttgttcaa
     4261 tatttggtaa aggaagtaaa tcagttccct tctgatatag aatgcatgag atacatagca
     4321 acaattacag ataaggaact gttgaaaaaa tgtcatcaat gtgtcgaaac cattgtgaag
     4381 gctaaagacc agcaagctgc agaagcaaat aagaatgcga gtattctttt aaaggaactt
     4441 gatctggaaa agtcaagaga agagagcaga aagcaggctc ttgctgctaa aagagaaaaa
     4501 agaaaagaaa agagaaaaaa gaaaaaagag gaacagaaaa ggaaacagga agaagatgaa
     4561 gaaaacaaac ctaaggagaa ttcggaacta ccagaggatg aagatgaaga ggagaatgat
     4621 gaagatgtgg agcaagaagt tcccatagaa cctcctagtg caaccaccac cactacgatt
     4681 ggaatctctg caacatctgc aacattcaca aatgtgtttg ggaaaaaaag ggccaatgtg
     4741 gtgacaactc ccagcaccaa tcggaaaaat aagaagaaca aaacaaaaga aacccctcct
     4801 acagcacatt taattttacc agaacaacat atgtctttag cccaacaaaa ggcagataaa
     4861 aataaaataa atggagaacc tagaggtggt ggtgcaggtg ggaatagtga ttcagataac
     4921 ttggacagca cagactgcaa cagtgagagt agcagtggtg gtaaaagcca agagttaaat
     4981 tttgtgatgg atgtgaattc ctctaaatac ccctcactgc tccttcattc ccaagaagaa
     5041 aagacaagta ctgctacttc caaaactcag acacgacttg aaggtgaagt gactcctaat
     5101 tccttgtcaa ccagctacaa gacagtgtca ttgccattaa gctctccaaa cataaagctg
     5161 aatctcacta gccctaaaag gggtcagaaa agagaagaag ggtggaaaga agttgtacga
     5221 aggtcaaaga aattgtctgt tccagcctca gtggtgtcga ggataatggg aagaggagga
     5281 tgcaacatca ctgcaataca ggatgttact ggtgcccata ttgatgtgga taaacaaaaa
     5341 gataagaatg gcgagagaat gatcacaata aggggtggca cagaatcaac aagatatgca
     5401 gttcaactaa tcaatgcact cattcaagat cctgctaagg aactggaaga cttgattcct
     5461 aaaaatcata tcagaacacc tgccagcacc aaatcaattc atgctaactt ctcatctgga
     5521 gtaggtacca cagcagcttc cagtaaaaat gcatttcctt tgggtgctcc aactcttgta
     5581 acttcacagg caacaacgtt atctacgttc cagcccgcta ataaacttaa taagaatgtt
     5641 ccaacaaatg tacgttcttc tttcccagtt tctctaccct tagcttatcc tcaccctcat
     5701 tttgccctgc tggctgctca aactatgcaa cagattcggc atcctcgctt acccatggcc
     5761 cagtttggag gaaccttctc accttctcct aacacatggg gaccattccc agtgagacct
     5821 gtgaatcctg gcaacacaaa tagctctcca aagcataata acacaagccg tctacctaac
     5881 cagaacggga ctgttttacc ctcagagtct gctggactag ctactgccag ttgtcctatc
     5941 actgtctctt ctgtagttgc tgccagtcag caactgtgtg tcactaatac ccggactcct
     6001 tcatcagtca gaaagcagtt gtttgcctgt gtgcctaaga caagtcctcc agcaacagtg
     6061 atttcttctg tgacaagcac ttgtagttcc ctgccttctg tctcctctgc acctatcact
     6121 agcgggcaag ctcccaccac atttctacct gcaagtactt ctcaagcaca gctttcttca
     6181 caaaagatgg agtctttctc tgctgtgcca cccaccaaag agaaagtgtc cacacaggac
     6241 cagcccatgg caaacctatg taccccatct tcaactgcaa acagttgcag tagctctgcc
     6301 agcaacaccc cgggagctcc agaaactcac ccatccagta gtcccactcc tacttccagt
     6361 aacacacaag aggaggcaca gccatccagt gtgtctgatt taagtcctat gtcaatgcct
     6421 tttgcatcta actcagaacc tgctccattg actttgacat cacccagaat ggttgctgct
     6481 gataatcagg acaccagtaa tttacctcag ttagctgtac cagcacctcg agtttctcat
     6541 cgaatgcagc ccagaggttc tttttactcc atggtaccaa atgcaactat tcaccaggat
     6601 ccccagtcta tttttgttac gaatccagtt actttaacac cacctcaagg cccaccagct
     6661 gcagtgcagc tttcttcagc tgtgaacatt atgaatggtt ctcagatgca cataaaccca
     6721 gcaaataagt ctttgccacc tacatttggc ccagccacac ttttcaatca cttcagcagt
     6781 ctttttgata gtagtcaggt gccagctaac cagggctggg gagatggtcc actgtcctca
     6841 cgagttgcta cagatgcctc tttcactgtt cagtcagcgt tcctgggtaa ctcagtgctt
     6901 ggacacttgg aaaacatgca ccctgataac tcaaaggcac ctggcttcag accaccttcc
     6961 cagcgagttt ctactagtcc agttgggtta ccatccattg acccatcagg cagctcccca
     7021 tcttcctctt ctgctcctct ggcaagtttt tccggcatac caggaacaag ggttttcctg
     7081 caagggccag ctcctgttgg gactcctagt ttcaacagac aacatttttc tccccatcct
     7141 tggacaagcg cctcaaactc atccacttct gccccaccaa cgttgggcca accaaaagga
     7201 gtcagtgcca gtcaagatcg aaagatacct cccccaattg gaacagagag actggcccga
     7261 attcggcaag gagggtctgt tgcacaagcc ccggcgggga ccagttttgt cgctcccgtt
     7321 ggacacagtg gaatctggtc atttggtgtc aatgctgtgt cagaaggctt atcaggttgg
     7381 tcgcaatctg tgatggggaa ccatccaatg catcaacaat tatcagaccc aagcacattc
     7441 tcccaacatc agccaatgga gagagatgat tctggaatgg tagccccctc taacattttt
     7501 catcagccta tggcaagtgg ttttgtggat ttttctaaag gtctgccaat ttccatgtat
     7561 ggaggcacca taataccctc tcatcctcag cttgctgatg ttccaggagg ccctctgttt
     7621 aatggacttc acaatccaga tcctgcttgg aaccctatga taaaagttat ccaaaattca
     7681 actgaatgca ctgatgccca gcagatttgg cctggcacgt gggcacctca tattggaaac
     7741 atgcatctca aatatgtcaa ctaagttaga aggtctttac tctttagcct tgtttaagaa
     7801 acctatgacc ttggaagaac catggggatt tttttttaat gtgcctaaga aattttctct
     7861 gaggctttag caatggaaat ttgattgccc attgtataag aacaaattga tttcctatcc
     7921 acctgattat gttctctggt tagtttagcc attttgaact taagatcata tgaccttagt
     7981 gcttttggct aaacatacag aatactactt gtatgcagaa gagaattagt tgattacatg
     8041 tttcaacctt ttagggtgat aaatacatgt ataattgttt acatacttaa aaggaaaaag
     8101 ttgagtaaat ttcttgtcat atagtggctc tacgtaatgt agcctgtatt aatgtgaaat
     8161 atttaccaga atattcaata aaaagatgaa cagtc
//
