LOCUS       NM_001330063            7506 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens ankyrin repeat and FYVE domain containing 1 (ANKFY1),
            transcript variant 5, mRNA.
ACCESSION   NM_001330063 XM_005256679
VERSION     NM_001330063.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7506)
  AUTHORS   Tanaka,M., Nakamura,S., Maekawa,M., Higashiyama,S. and Hara,H.
  TITLE     ANKFY1 is essential for retinal endothelial cell proliferation and
            migration via VEGFR2/Akt/eNOS pathway
  JOURNAL   Biochem Biophys Res Commun 533 (4), 1406-1412 (2020)
   PUBMED   33092793
  REMARK    GeneRIF: ANKFY1 is essential for retinal endothelial cell
            proliferation and migration via VEGFR2/Akt/eNOS pathway.
REFERENCE   2  (bases 1 to 7506)
  AUTHORS   Bahl,K., Xie,S., Spagnol,G., Sorgen,P., Naslavsky,N. and Caplan,S.
  TITLE     EHD3 Protein Is Required for Tubular Recycling Endosome
            Stabilization, and an Asparagine-Glutamic Acid Residue Pair within
            Its Eps15 Homology (EH) Domain Dictates Its Selective Binding to
            NPF Peptides
  JOURNAL   J Biol Chem 291 (26), 13465-13478 (2016)
   PUBMED   27189942
REFERENCE   3  (bases 1 to 7506)
  AUTHORS   Nehru,V., Voytyuk,O., Lennartsson,J. and Aspenstrom,P.
  TITLE     RhoD binds the Rab5 effector Rabankyrin-5 and has a role in
            trafficking of the platelet-derived growth factor receptor
  JOURNAL   Traffic 14 (12), 1242-1254 (2013)
   PUBMED   24102721
  REMARK    GeneRIF: Rabankyrin-5 is required for internalization and
            trafficking of the PDGF receptor.
REFERENCE   4  (bases 1 to 7506)
  AUTHORS   McGrath,L.M., Cornelis,M.C., Lee,P.H., Robinson,E.B., Duncan,L.E.,
            Barnett,J.H., Huang,J., Gerber,G., Sklar,P., Sullivan,P.,
            Perlis,R.H. and Smoller,J.W.
  TITLE     Genetic predictors of risk and resilience in psychiatric disorders:
            a cross-disorder genome-wide association study of functional
            impairment in major depressive disorder, bipolar disorder, and
            schizophrenia
  JOURNAL   Am J Med Genet B Neuropsychiatr Genet 162B (8), 779-788 (2013)
   PUBMED   24039173
REFERENCE   5  (bases 1 to 7506)
  AUTHORS   Kang,D., Oh,S., Ahn,S.M., Lee,B.H. and Moon,M.H.
  TITLE     Proteomic analysis of exosomes from human neural stem cells by flow
            field-flow fractionation and nanoflow liquid chromatography-tandem
            mass spectrometry
  JOURNAL   J Proteome Res 7 (8), 3475-3480 (2008)
   PUBMED   18570454
REFERENCE   6  (bases 1 to 7506)
  AUTHORS   Schroder,B., Wrocklage,C., Pan,C., Jager,R., Kosters,B.,
            Schafer,H., Elsasser,H.P., Mann,M. and Hasilik,A.
  TITLE     Integral and associated lysosomal membrane proteins
  JOURNAL   Traffic 8 (12), 1676-1686 (2007)
   PUBMED   17897319
REFERENCE   7  (bases 1 to 7506)
  AUTHORS   Bouslam,N., Bouhouche,A., Benomar,A., Hanein,S., Klebe,S.,
            Azzedine,H., Di Giandomenico,S., Boland-Auge,A., Santorelli,F.M.,
            Durr,A., Brice,A., Yahyaoui,M. and Stevanin,G.
  TITLE     A novel locus for autosomal recessive spastic ataxia on chromosome
            17p
  JOURNAL   Hum Genet 121 (3-4), 413-420 (2007)
   PUBMED   17273843
REFERENCE   8  (bases 1 to 7506)
  AUTHORS   Schnatwinkel,C., Christoforidis,S., Lindsay,M.R.,
            Uttenweiler-Joseph,S., Wilm,M., Parton,R.G. and Zerial,M.
  TITLE     The Rab5 effector Rabankyrin-5 regulates and coordinates different
            endocytic mechanisms
  JOURNAL   PLoS Biol 2 (9), E261 (2004)
   PUBMED   15328530
  REMARK    GeneRIF: Rab5 regulates and coordinates different endocytic
            mechanisms through its effector Rabankyrin-5
REFERENCE   9  (bases 1 to 7506)
  AUTHORS   Kuriyama,H., Asakawa,S., Minoshima,S., Maruyama,H., Ishii,N.,
            Ito,K., Gejyo,F., Arakawa,M., Shimizu,N. and Kuwano,R.
  TITLE     Characterization and chromosomal mapping of a novel human gene,
            ANKHZN
  JOURNAL   Gene 253 (2), 151-160 (2000)
   PUBMED   10940552
REFERENCE   10 (bases 1 to 7506)
  AUTHORS   Ito,K., Ishii,N., Miyashita,A., Tominaga,K., Kuriyama,H.,
            Maruyama,H., Shirai,M., Naito,M., Arakawa,M. and Kuwano,R.
  TITLE     Molecular cloning of a novel 130-kDa cytoplasmic protein, Ankhzn,
            containing Ankyrin repeats hooked to a zinc finger motif
  JOURNAL   Biochem Biophys Res Commun 257 (1), 206-213 (1999)
   PUBMED   10092534
  REMARK    GeneRIF: Characterization of the related mouse gene
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC087742.9, AC087292.14 and
            AA897044.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001330063.1.
            
            Summary: This gene encodes a cytoplasmic protein that contains a
            coiled-coil structure and a BTB/POZ domain at its N-terminus,
            ankyrin repeats in the middle portion, and a FYVE-finger motif at
            its C-terminus. This protein belongs to a subgroup of double zinc
            finger proteins which may be involved in vesicle or protein
            transport. Alternate splicing results in multiple transcript
            variants of this gene. [provided by RefSeq, Apr 2012].
            
            Transcript Variant: This variant (5) uses an alternate in-frame
            splice site in the 3' coding region compared to variant 1. The
            encoded isoform (4) is shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.69659.1,
                                           SRR1660807.197580.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000341657.9/ ENSP00000343362.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-48                AC087742.9         55891-55938
            49-241              AC087742.9         77422-77614
            242-360             AC087742.9         83980-84098
            361-496             AC087742.9         102752-102887
            497-620             AC087742.9         109923-110046
            621-770             AC087292.14        194985-195134       c
            771-936             AC087292.14        193373-193538       c
            937-1141            AC087292.14        184425-184629       c
            1142-1210           AC087292.14        182455-182523       c
            1211-1410           AC087292.14        182030-182229       c
            1411-1508           AC087292.14        176434-176531       c
            1509-1737           AC087292.14        171870-172098       c
            1738-1836           AC087292.14        170864-170962       c
            1837-1990           AC087292.14        170450-170603       c
            1991-2159           AC087292.14        169233-169401       c
            2160-2278           AC087292.14        168306-168424       c
            2279-2435           AC087292.14        166773-166929       c
            2436-2636           AC087292.14        165909-166109       c
            2637-2813           AC087292.14        164178-164354       c
            2814-2961           AC087292.14        160961-161108       c
            2962-3052           AC087292.14        160406-160496       c
            3053-3177           AC087292.14        159608-159732       c
            3178-3324           AC087292.14        157766-157912       c
            3325-3415           AC087292.14        156249-156339       c
            3416-7312           AC087292.14        151066-154962       c
            7313-7506           AA897044.1         17-210              c
FEATURES             Location/Qualifiers
     source          1..7506
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.2"
     gene            1..7506
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /note="ankyrin repeat and FYVE domain containing 1"
                     /db_xref="GeneID:51479"
                     /db_xref="HGNC:HGNC:20763"
                     /db_xref="MIM:607927"
     exon            1..48
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     CDS             39..3548
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /note="isoform 4 is encoded by transcript variant 5;
                     ankyrin repeat hooked to zinc finger motif; Rabankyrin-5;
                     rank-5; ankyrin repeat and FYVE domain-containing protein
                     1; ankyrin repeats hooked to a zinc finger motif"
                     /codon_start=1
                     /product="rabankyrin-5 isoform 4"
                     /protein_id="NP_001316992.1"
                     /db_xref="CCDS:CCDS82038.1"
                     /db_xref="GeneID:51479"
                     /db_xref="HGNC:HGNC:20763"
                     /db_xref="MIM:607927"
                     /translation="MAEEEVAKLEKHLMLLRQEYVKLQKKLAETEKRCALLAAQANKE
                     SSSESFISRLLAIVADLYEQEQYSDLKIKVGDRHISAHKFVLAARSDSWSLANLSSTK
                     ELDLSDANPEVTMTMLRWIYTDELEFREDDVFLTELMKLANRFQLQLLRERCEKGVMS
                     LVNVRNCIRFYQTAEELNASTLMNYCAEIIASHWDDLRKEDFSSMSAQLLYKMIKSKT
                     EYPLHKAIKVEREDVVFLYLIEMDSQLPGKLNEADHNGDLALDLALSRRLESIATTLV
                     SHKADVDMVDKSGWSLLHKGIQRGDLFAATFLIKNGAFVNAATLGAQETPLHLVALYS
                     SKKHSADVMSEMAQIAEALLQAGANPNMQDSKGRTPLHVSIMAGNEYVFSQLLQCKQL
                     DLELKDHEGSTALWLAVQHITVSSDQSVNPFEDVPVVNGTSFDENSFAARLIQRGSHT
                     DAPDTATGNCLLQRAAGAGNEAAALFLATNGAHVNHRNKWGETPLHTACRHGLANLTA
                     ELLQQGANPNLQTEEALPLPKEAASLTSLADSVHLQTPLHMAIAYNHPDVVSVILEQK
                     ANALHATNNLQIIPDFSLKDSRDQTVLGLALWTGMHTIAAQLLGSGAAINDTMSDGQT
                     LLHMAIQRQDSKSALFLLEHQADINVRTQDGETALQLAIRNQLPLVVDAICTRGADMS
                     VPDEKGNPPLWLALANNLEDIASTLVRHGCDATCWGPGPGGCLQTLLHRAIDENNEPT
                     ACFLIRSGCDVNSPRQPGANGEGEEEARDGQTPLHLAASWGLEETVQCLLEFGANVNA
                     QDAEGRTPIHVAISSQHGVIIQLLVSHPDIHLNVRDRQGLTPFACAMTFKNNKSAEAI
                     LKRESGAAEQVDNKGRNFLHVAVQNSDIESVLFLISVHANVNSRVQDASKLTPLHLAV
                     QAGSEIIVRNLLLAGAKVNELTKHRQTALHLAAQQDLPTICSVLLENGVDFAAVDENG
                     NNALHLAVMHGRLNNIRVLLTECTVDAEAFNLRGQSPLHILGQYGKENAAAIFDLFLE
                     CMPGYPLDKPDADGSTVLLLAYMKGNANLCRAIVRSGARLGVNNNQGVNIFNYQVATK
                     QLLFRLLDMLSKEPPWCDGSYCYECTARFGVTTRKHHCRHCGRLLCHKCSTKEIPIIK
                     FDLNKPVRVCNICFDVLTLGGVS"
     exon            49..241
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            242..360
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            361..496
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            497..620
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            621..770
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            771..936
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1141
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..1210
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..1410
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1508
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            1509..1737
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            1738..1836
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            1837..1990
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            1991..2159
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            2160..2278
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            2279..2435
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            2436..2636
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            2637..2813
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            2814..2961
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            2962..3052
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            3053..3177
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            3178..3324
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            3325..3415
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     exon            3416..7506
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7399..7404
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /note="hexamer: AATAAA"
     polyA_site      7422
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
     regulatory      7485..7490
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /note="hexamer: ATTAAA"
     polyA_site      7506
                     /gene="ANKFY1"
                     /gene_synonym="ANKHZN; BTBD23; ZFYVE14"
                     /note="major polyA site"
ORIGIN      
        1 gcacttctcg cgaggttgca ggcagtgccg ggccagacat ggcggaagag gaggtggcca
       61 agttggagaa gcacttgatg cttctgcggc aggagtatgt caagctgcag aagaagctgg
      121 cggagacaga gaagcgctgc gctctcttgg ctgcgcaggc aaacaaggag agcagcagcg
      181 agtccttcat cagccgtctg ctggccatcg tggcagacct ctacgagcag gagcagtaca
      241 gcgatctgaa gataaaggtt ggggacaggc acatcagtgc tcacaagttt gtcctggcag
      301 cccgcagtga cagctggagt ctggctaact tgtcttccac taaagagttg gacctgtcag
      361 atgctaatcc tgaggtgacg atgacaatgc ttcgctggat ctatacagat gagctggagt
      421 tcagagagga tgatgtgttc ctgactgaac tgatgaaact agcaaatcgg tttcagctac
      481 agctcctcag ggagagatgt gagaagggtg ttatgtctct agtgaatgtc aggaactgta
      541 ttcgcttcta ccagacggca gaggagctga atgccagcac actgatgaac tactgtgcag
      601 aaattattgc aagtcattgg gacgacctga ggaaggagga tttcagcagc atgagcgctc
      661 agttgttata caaaatgatc aaatccaaga cagagtaccc gctacataaa gccatcaaag
      721 tggagagaga agacgtggtc ttcctgtatc tgattgaaat ggattcccag ctccctggga
      781 agctgaatga agcggatcat aacggagatc tggcattaga tctagccctc tcacgacgac
      841 tggagagtat tgccaccacg ctggttagtc acaaagctga tgtggacatg gtggacaaga
      901 gtggctggag cttgttacac aaaggaatcc aaagaggaga tctctttgct gccactttcc
      961 tcattaagaa tggggccttt gtcaacgctg ctacactggg tgcccaggag acaccactgc
     1021 accttgtggc cttgtacagt tcaaagaaac actcagcaga tgtgatgtct gagatggcgc
     1081 agattgcaga ggcccttctg caggctggtg ccaaccccaa catgcaggac agcaagggga
     1141 ggactccttt acatgtgtcc atcatggccg ggaatgaata tgtgttcagt cagctgctgc
     1201 agtgcaaaca actagattta gaactcaaag accacgaggg cagcacggct ctgtggctgg
     1261 cagtgcagca tatcacagtg tcttctgacc agtctgtgaa ccccttcgaa gatgtccccg
     1321 tggtaaatgg gacttcattt gatgagaaca gctttgcagc cagactcatc cagcgcggca
     1381 gccacacaga cgcacctgac acggcgacag gaaactgttt actacagcgg gcagctggag
     1441 caggaaacga ggcagcagct cttttcctgg caaccaacgg tgcccatgtc aaccacagaa
     1501 acaagtgggg agaaaccccg ttgcacacag cgtgtcggca tggcctggcc aacctcacgg
     1561 cagagctcct gcagcaaggc gccaacccaa acctgcagac ggaggaagct ctgcctctgc
     1621 caaaggaggc cgcatccctg accagcttgg cggacagcgt ccatctgcag acgccactgc
     1681 acatggcgat cgcctataac catccggatg tggtgtctgt catcctggag cagaaagcca
     1741 atgctcttca tgccaccaac aacttgcaga tcattccgga cttcagcctc aaagattccc
     1801 gagaccagac tgtgctgggc ctggcattat ggactggcat gcacacgatc gcagcccagc
     1861 tgctgggctc tggagccgcc atcaatgaca ccatgtcgga tgggcagacg ctactgcaca
     1921 tggccataca gcggcaggac agcaagagcg cactcttcct gctggagcac caggcagata
     1981 taaatgtcag gactcaggac ggggagacag ccctccagct ggccatcaga aaccagcttc
     2041 cactcgtagt tgatgccata tgcacccgag gagctgacat gtctgtgcca gatgagaagg
     2101 ggaacccccc gctgtggctt gcattggcaa acaatctgga ggacatcgca tccactctgg
     2161 tcagacatgg ctgtgatgcc acatgctggg gtccgggacc tggtgggtgc cttcagacgc
     2221 tcctgcacag agccattgat gaaaacaacg agcccaccgc ctgctttctt attcgcagtg
     2281 gctgtgacgt gaacagtccc agacaaccag gcgccaatgg agaaggagag gaagaggcta
     2341 gagatgggca gacccctttg catttggcag cctcttgggg gctggaagag acagtacagt
     2401 gtcttctgga gtttggtgcc aacgtgaacg cacaggatgc agaaggaaga acccccatcc
     2461 acgtggccat cagcagccaa cacggtgtca tcattcagct gttggtttct caccccgata
     2521 tccatttgaa tgtacgagac agacaagggc tgaccccgtt tgcctgtgcc atgactttca
     2581 agaacaacaa gtcagccgag gccattctca aacgagagtc cggggctgct gagcaggtgg
     2641 ataacaaggg ccggaatttc cttcatgtgg cagttcagaa ctctgatatt gaaagtgtgc
     2701 tgttcctgat cagtgtccac gctaatgtga attcaagagt ccaggatgcc tccaagttga
     2761 cccccctgca cctcgctgtc caagcaggct cagaaattat tgtccgcaat ttgcttcttg
     2821 cgggagccaa agtgaacgaa ttaaccaagc atcgccagac tgccctccat cttgctgccc
     2881 agcaggacct gcccaccatc tgctcagtcc tcctagagaa tggcgtggac tttgctgccg
     2941 tggatgagaa tggaaacaat gctcttcatc ttgctgtcat gcacggccgg ctcaacaaca
     3001 tccgggttct cctgacagag tgcacagtgg acgccgaagc ctttaatctc agaggccagt
     3061 caccactgca cattttggga caatatggca aggagaatgc agcggccatc tttgatctct
     3121 tcctagaatg catgccgggg tatcctctgg acaagccgga tgcagacggc agcacggtgc
     3181 tgctcctggc atacatgaaa gggaacgcca acttgtgccg cgccatcgtc cggtcggggg
     3241 ctcgcctcgg ggtgaataac aaccagggag tcaacatctt caactaccag gtcgccacca
     3301 agcagctcct gttccgactg ctggatatgc tgtccaagga gcctccgtgg tgtgacggct
     3361 cctactgcta tgagtgcact gccaggttcg gagtcaccac tcgcaaacac cactgtcgtc
     3421 actgcggacg tcttctttgc cataaatgct cgaccaagga gattcctatt ataaagtttg
     3481 atctgaacaa gcctgtgcgg gtttgcaaca tttgttttga tgtactgact ctgggtgggg
     3541 tttcttagtg agccccccgg agggtccagg ccacgtcctt ggtcacctcc ccagcagctg
     3601 ctctgctcac cagcctgacc ccacccagag caggagctgg cgggtgtctt cctgcggcaa
     3661 tagactggaa cgattaagga ccatggtgtg atagatccca tttcaaatga ttccatatga
     3721 ttgtcagtgt gtgtgtcaga ctgtgatcga tttcactaga tgtctcactc atcagaccag
     3781 gccattgggc ctaagtggta aatgtgatta ggaattagac ctctccccat tctgctagca
     3841 acatacaggg acacttggaa aaccatttcc ccttccagta gcttggtgtc catcccagag
     3901 cattcatgaa gtcttcccgc ctgggctgac tgatgaggca gagcctctca gcgtaggaag
     3961 ctggctgctt aatgagctgg ctttactcta gggtaagtgg ctgtggactt ttctgcacag
     4021 tgttttcata aagataatag gatcctcttg ccctgaagtc tttttttctt tttacgctaa
     4081 gctgtatttt tagtgagcta ccccttttaa aaaggtgaaa tctttcttaa cagggttcaa
     4141 agatgagagc tgaaaaatcg tggccttaac aactgaaagc tttacagtgt tcatgctact
     4201 gaggtgtcag gagtgcagct gggccgcttg acacctcgta gcagccgtct cattctctcg
     4261 tcttgctgcg tcctgtctgt ggagtcctca gtcactctgc tgttggagag tgctggagga
     4321 aatgcacttt tactgtgctg cactttttat agagctgcat tgtcggtgat tccaatttaa
     4381 aaatccatat tcaaaaatac cctcacacat ccctgcatca gtgattctaa tgatcagctt
     4441 tgactggacc tcagctcaca cgaatctcca aggaaaaact ctacagagca tcttttagag
     4501 caagagattc atacaggcca agtgatgaga gggggagtca ccaaggcttc agccccaagg
     4561 gcatctccag cctcaggccg gcaggtccta tgtccttgac acaaatgctt cttgctgaaa
     4621 tcctccctag tgatgctgcg agtgctgttg gtcccagttc tgtgtagctt gccctgcccc
     4681 atgaagggcc accagccaag ggccctggcc acgtgtgcgc caccttactt tggaagtctc
     4741 tggcttgtgt gctggatgag gctgccagaa cttgttgggc gttcatcgct gttagtacat
     4801 atattaaccg tgaggtgtta aacttttctt ttgaaggttt ggccagtttc taaaaaatgc
     4861 acatttaaag agaagcatct accacggctt taaaacaaaa caactctgag atgaacaata
     4921 tgtgttatac tcagagatta acaatctcaa tcatacatac tgattctttc agacatttaa
     4981 taaccactac atttttttgc attaatgaag tttgactata tgtgtaaagg gactaaatat
     5041 ttttgcaaca gcctgttctt tgttcattct tttctggata gtgtgtcctc tgtattgcgg
     5101 tagatttata cattctgttg cctaaatatg tgtgtaaaat gagctgataa actggagtac
     5161 tacttaaaaa aaagtctgtg atttataaga tgtatatgct ttctatgtga atataagctt
     5221 gtgcacaatg tttaaaagaa aaacaatgaa ttagaagaga tcccccgtcc cccagtctga
     5281 catatttcat acagaatgtt taaaagaaaa actctgctag tcttggcaaa catttggcca
     5341 tgtgaggcat gtggtcagtt tccattttct attacctgga aagggccata aggaagcagg
     5401 cagggcttag gtagcttggt tacctaagtt agaaagtaag agaatcatga ttcggatatg
     5461 aattgaagtg gttggtacat ggatacctgt tctttaaaag gaatgtggga gatgagtcaa
     5521 gggtgaggcc gattgttctg cccatgccct accataccaa tttcaataac aatcttgcct
     5581 gtcattgcac tcagaatctt aagatcatgt ttcaaatatg ttgattcaaa cttgcttcta
     5641 agcctgagtc cagggatagc aatggtactg accagctatg gactgggaga acattgagtc
     5701 ctgttttcca ttggcttcag ggcgcatcca tgtggatata ggaaaagcct ctttccacag
     5761 acttaggaag ccccccgacc acgcggacgc tcgctgggct gcactcagca ggctgcttga
     5821 gtgggagctg actccaacat gccctgccct ggctgggact aggcatgctt ccagcccttc
     5881 ccgtgctctc cctgctgtca acacttgcgt acaaacctgc ctagctgttg taagttttca
     5941 tcagtagctt aagtactagt agcaaaaact taaattcatt cagaaagttg ccacttctga
     6001 atgagtagag caggaaaagt aaactttact gaaacataat ttgctgcaac agtcaacatt
     6061 gttatgctga atgtgctctc aactgccctt tgctcatcgc cagatttatt atccagtgta
     6121 ttcctaacag tcttttgttg tcgttgtttt taaaacaaag gtacggacca tgtgctatat
     6181 ataaatcgaa tggcttaaac taatttgcta tgatcctcta acaccgaaat ttcccataaa
     6241 cttaagtgcc tacacttagg acttttgaac tttcagtcat ctttttcgtg gtgtctcact
     6301 gaacgatgtg ttagaacatg tgttccattt ggagttttgg gcctgtgcac ggtcaacccc
     6361 aattaccaat ttagttgagg ctggattcga ttgctcagtt cacctgccac gttctctcca
     6421 aggagtgggg tctacgtgac ctttagtcct ctacctcaga gccccgagtc atttctgacc
     6481 accccgttct gtgtcctcac tcttgtccct gaatgggtcc ctgtgtggat ctcagtgtgt
     6541 gtgtggtttc tccactcctc cccgctcatg tcccacacct gccatattga accgtttctg
     6601 cactaatctt ctccacgggc acggagtgga gggaacgtct tgggaaaggg gagagcttga
     6661 cctccatcta ggtttctttt atctggagaa aaagaacact tttgaactat gtaatgcttc
     6721 accctgaaag gcaagctaac gctaacttcc caggtgacag tagcaggaac aaggaagggt
     6781 aatgtttcca tgacagacac ttgcttccct tgggacaagt cccagaagaa ctacctgaag
     6841 caccaaagct ccccacccca gcctggtggc agaggaagca ggtggcacca actcaccaaa
     6901 gggacttggg agccaccttc tcactctgtg gaagtacttt agtgagagct gactggcaag
     6961 ataaaaaccc tgaaattccc agaagttaca gatgccttaa agactttctt tggaagatca
     7021 ttgcctgtac tattcctttt ttgtgtggca taaataatat attaacctgt tacaactgac
     7081 aacattggtt ttctgcagct agggagtctt gagtcgagac tgctcgcctt gatgacttgc
     7141 tcttttttgg cagatgtgtg gagttggctg ttacgacatc cctgtgtgat cccagaggag
     7201 gggcagtagc tgggcagaaa ctgcaggcac acatttgggt ggatcacctg gcttccagcc
     7261 ccttcggggt ggtgctcagt gtggggtgat ggtatcatag aggaccacgg cgcttatcag
     7321 cattgtttct ttggagtctg tgtaatggaa tgtatttttc aaatactgat atttgcattt
     7381 tctgtaacaa ttccttataa taaaatgaag taaaattgtg catttaaagt ggaaacttta
     7441 ataagacatt tgtagttact tctaatgcag aagtaccaat aagcattaaa aacaaatttt
     7501 agaaaa
//
