LOCUS       NM_000037               8292 bp    mRNA    linear   PRI 01-JUL-2024
DEFINITION  Homo sapiens ankyrin 1 (ANK1), transcript variant 3, mRNA.
ACCESSION   NM_000037
VERSION     NM_000037.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8292)
  AUTHORS   Antikainen,A.A., Haukka,J.K., Kumar,A., Syreeni,A.,
            Hagg-Holmberg,S., Ylinen,A., Kilpelainen,E., Kytola,A., Palotie,A.,
            Putaala,J., Thorn,L.M., Harjutsalo,V., Groop,P.H. and Sandholm,N.
  CONSRTM   FinnDiane Study Group
  TITLE     Whole-genome sequencing identifies variants in ANK1, LRRN1, HAS1,
            and other genes and regulatory regions for stroke in type 1
            diabetes
  JOURNAL   Sci Rep 14 (1), 13453 (2024)
   PUBMED   38862513
  REMARK    GeneRIF: Whole-genome sequencing identifies variants in ANK1,
            LRRN1, HAS1, and other genes and regulatory regions for stroke in
            type 1 diabetes.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8292)
  AUTHORS   Lu,Z., Zhang,H., Yang,Y. and Zhao,H.
  TITLE     Sex differences of the shared genetic landscapes between type 2
            diabetes and peripheral artery disease in East Asians and Europeans
  JOURNAL   Hum Genet 142 (7), 965-980 (2023)
   PUBMED   37341850
  REMARK    GeneRIF: Sex differences of the shared genetic landscapes between
            type 2 diabetes and peripheral artery disease in East Asians and
            Europeans.
REFERENCE   3  (bases 1 to 8292)
  AUTHORS   More,T.A., Devendra,R., Dongerdiye,R., Warang,P. and Kedar,P.
  TITLE     Targeted next-generation sequencing identifies novel deleterious
            variants in ANK1 gene causing severe hereditary spherocytosis in
            Indian patients: expanding the molecular and clinical spectrum
  JOURNAL   Mol Genet Genomics 298 (2), 427-439 (2023)
   PUBMED   36598564
  REMARK    GeneRIF: Targeted next-generation sequencing identifies novel
            deleterious variants in ANK1 gene causing severe hereditary
            spherocytosis in Indian patients: expanding the molecular and
            clinical spectrum.
REFERENCE   4  (bases 1 to 8292)
  AUTHORS   Wang,Y., Huang,L., Zhu,Y., An,X., Li,J., Zhen,J. and Yu,J.
  TITLE     De novo variations of ANK1 gene caused hereditary spherocytosis in
            two Chinese children by affecting pre-mRNA splicing
  JOURNAL   BMC Pediatr 23 (1), 23 (2023)
   PUBMED   36647015
  REMARK    GeneRIF: De novo variations of ANK1 gene caused hereditary
            spherocytosis in two Chinese children by affecting pre-mRNA
            splicing.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8292)
  AUTHORS   Xu,L., Wei,X., Liang,G., Zhu,D., Zhang,Y., Zhang,Y. and Shang,X.
  TITLE     A novel splicing mutation of ANK1 is associated with phenotypic
            heterogeneity of hereditary spherocytosis in a Chinese family
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1869 (1), 166595 (2023)
   PUBMED   36336297
  REMARK    GeneRIF: A novel splicing mutation of ANK1 is associated with
            phenotypic heterogeneity of hereditary spherocytosis in a Chinese
            family.
REFERENCE   6  (bases 1 to 8292)
  AUTHORS   Fujimoto,T., Lee,K., Miwa,S. and Ogawa,K.
  TITLE     Immunocytochemical localization of fodrin and ankyrin in bovine
            chromaffin cells in vitro
  JOURNAL   J Histochem Cytochem 39 (11), 1485-1493 (1991)
   PUBMED   1833445
REFERENCE   7  (bases 1 to 8292)
  AUTHORS   Lux,S.E., John,K.M. and Bennett,V.
  TITLE     Analysis of cDNA for human erythrocyte ankyrin indicates a repeated
            structure with homology to tissue-differentiation and cell-cycle
            control proteins
  JOURNAL   Nature 344 (6261), 36-42 (1990)
   PUBMED   2137557
REFERENCE   8  (bases 1 to 8292)
  AUTHORS   Lambert,S., Yu,H., Prchal,J.T., Lawler,J., Ruff,P., Speicher,D.,
            Cheung,M.C., Kan,Y.W. and Palek,J.
  TITLE     cDNA sequence for human erythrocyte ankyrin
  JOURNAL   Proc Natl Acad Sci U S A 87 (5), 1730-1734 (1990)
   PUBMED   1689849
REFERENCE   9  (bases 1 to 8292)
  AUTHORS   Bennett,V.
  TITLE     Immunoreactive forms of human erythrocyte ankyrin are present in
            diverse cells and tissues
  JOURNAL   Nature 281 (5732), 597-599 (1979)
   PUBMED   492324
REFERENCE   10 (bases 1 to 8292)
  AUTHORS   Bennett,V. and Stenbuck,P.J.
  TITLE     The membrane attachment protein for spectrin is associated with
            band 3 in human erythrocyte membranes
  JOURNAL   Nature 280 (5722), 468-473 (1979)
   PUBMED   379653
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from M28880.1, AB209418.1, X16609.1,
            AC113133.5 and N63745.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_000037.3.
            
            Summary: Ankyrins are a family of proteins that link the integral
            membrane proteins to the underlying spectrin-actin cytoskeleton and
            play key roles in activities such as cell motility, activation,
            proliferation, contact and the maintenance of specialized membrane
            domains. Multiple isoforms of ankyrin with different affinities for
            various target proteins are expressed in a tissue-specific,
            developmentally regulated manner. Most ankyrins are typically
            composed of three structural domains: an amino-terminal domain
            containing multiple ankyrin repeats; a central region with a highly
            conserved spectrin binding domain; and a carboxy-terminal
            regulatory domain which is the least conserved and subject to
            variation. Ankyrin 1, the prototype of this family, was first
            discovered in the erythrocytes, but since has also been found in
            brain and muscles. Mutations in erythrocytic ankyrin 1 have been
            associated in approximately half of all patients with hereditary
            spherocytosis. Complex patterns of alternative splicing in the
            regulatory domain, giving rise to different isoforms of ankyrin 1
            have been described. Truncated muscle-specific isoforms of ankyrin
            1 resulting from usage of an alternate promoter have also been
            identified. [provided by RefSeq, Dec 2008].
            
            Transcript Variant: This variant (3) has an alternate segment in
            the 3' coding region, which includes a stop codon, compared to
            variant 1. The resulting isoform (3) has a distinct and one aa
            shorter C-terminus, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M28880.1, SRR14038196.1349941.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000289734.13/ ENSP00000289734.8
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-398               M28880.1           1-398
            399-400             AB209418.1         696-697
            401-770             M28880.1           401-770
            771-774             X16609.1           771-774
            775-3029            M28880.1           775-3029
            3030-3032           X16609.1           3030-3032
            3033-3307           M28880.1           3033-3307
            3308-3571           AB209418.1         3629-3892
            3572-4184           M28880.1           3572-4184
            4185-4186           AB209418.1         4506-4507
            4187-4719           M28880.1           4187-4719
            4720-4721           X16609.1           4720-4721
            4722-6157           M28880.1           4722-6157
            6158-8291           AC113133.5         6261-8394           c
            8292-8292           N63745.1           9-9                 c
FEATURES             Location/Qualifiers
     source          1..8292
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p11.21"
     gene            1..8292
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /note="ankyrin 1"
                     /db_xref="GeneID:286"
                     /db_xref="HGNC:HGNC:492"
                     /db_xref="MIM:612641"
     exon            1..111
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     CDS             85..5727
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /note="isoform 3 is encoded by transcript variant 3;
                     ankyrin-R; ANK-1; erythrocyte ankyrin; ankyrin 1,
                     erythrocytic"
                     /codon_start=1
                     /product="ankyrin-1 isoform 3"
                     /protein_id="NP_000028.3"
                     /db_xref="CCDS:CCDS6121.1"
                     /db_xref="GeneID:286"
                     /db_xref="HGNC:HGNC:492"
                     /db_xref="MIM:612641"
                     /translation="MPYSVGFREADAATSFLRAARSGNLDKALDHLRNGVDINTCNQN
                     GLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGA
                     NVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVV
                     AHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYEN
                     LNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPL
                     HCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDI
                     TLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLK
                     TGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHT
                     EVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLH
                     IAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAG
                     KNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQY
                     GGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVP
                     VADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQ
                     AAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVLKVVTDETSFVLV
                     SDKHRMSFPETVDEILDVSEDEGEELISFKAERRDSRDVDEEKELLDFVPKLDQVVES
                     PAIPRIPCAMPETVVIRSEEQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFL
                     VSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLSTPPPLAEEEGLA
                     SRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHRSRYGESYLD
                     QILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLKSKLV
                     PLVQATFPENAVTKRVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIG
                     LRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLVYANECANFT
                     TNVSARFWLSDCPRTAEAVNFATLLYKELTAVPYMAKFVIFAKMNDPREGRLRCYCMT
                     DDKVDKTLEQHENFVEVARSRDIEVLEGMSLFAELSGNLVPVKKAAQQRSFHFQSFRE
                     NRLAMPVKVRDSSREPGGSLSFLRKAMKYEDTQHILCHLNITMPPCAKGSGAEDRRRT
                     PTPLALRYSILSESTPGSLSGTEQAEMKMAVISEHLGLSWAELARELQFSVEDINRIR
                     VENPNSLLEQSVALLNLWVIREGQNANMENLYTALQSIDRGEIVNMLEGSGRQSRNLK
                     PDRRHTDRDYSLSPSQMNGYSSLQDELLSPASLGCALSSPLRADQYWNEVAVLDAIPL
                     AATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDATGHEWKLEGALSEEP
                     RGPELGSLELVEDDTVDSDATNGLIDLLEQEEGQRSEEKLPGSKRQDDATGAGQDSEN
                     EVSLVSGHQRGQARITHSPTVSQVTERSQDRLQDWDADGSIVSYLQDAAQGSWQEEVT
                     QGPHSFQGTSTMTEGLEPGGSQEYEKVLVSVSEHTWTEQPEAESSQADRDRRQQGQEE
                     QVQEAKNTFTQVVQGNEFQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQIDLSSADA
                     AQEHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ"
     exon            112..213
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            214..312
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            313..411
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            412..510
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            511..696
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            697..795
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            796..894
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            895..993
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1191
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1192..1290
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1291..1389
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1488
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1489..1686
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1687..1785
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1786..1884
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1885..2082
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2083..2181
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2182..2280
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2281..2379
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2380..2472
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2473..2545
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2546..2642
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2643..2721
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2722..2819
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2820..3044
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            3045..3199
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            3200..3411
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            3412..3616
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            3617..3713
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            3714..3942
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            3943..4068
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            4069..4188
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            4189..4267
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            4268..4342
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            4343..4474
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            4475..4621
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            4622..5180
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            5181..5478
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            5479..5562
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            5563..5628
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            5629..5763
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     exon            5764..8292
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6797..6802
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /note="hexamer: AATAAA"
     polyA_site      6817
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /note="major polyA site"
     regulatory      8270..8275
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /note="hexamer: AATAAA"
     polyA_site      8292
                     /gene="ANK1"
                     /gene_synonym="ANK; ankyrin-1; SPH1; SPH2"
                     /note="major polyA site"
ORIGIN      
        1 agaggctgcg gtgagtccgc cagccccagc tgctcctcct caagccccca aggcccttcg
       61 gcgggcccct gctgaaagac cggcatgccc tattctgtgg gcttccgcga agccgatgct
      121 gctaccagct ttctgagagc agcaagatca ggtaacttgg acaaagcttt ggatcacctg
      181 cggaatgggg tagatattaa cacctgtaac cagaatgggt tgaatggctt gcatctggct
      241 tctaaggaag gccatgtgaa aatggtggtt gaacttctgc acaaagaaat cattctagaa
      301 acgacaacca agaaggggaa cacggccctg cacatcgctg ctctagccgg gcaggatgag
      361 gtggtccggg agcttgtcaa ctatggagcc aacgtcaacg cccagtcaca gaaaggtttt
      421 acacccctgt acatggcagc acaagagaac cacttggaag tggttaagtt tttactggaa
      481 aatggagcta accagaatgt agccacagaa gacggcttca cgcctctggc ggtagccctg
      541 cagcagggcc atgagaacgt cgtcgcgcac ctcatcaact acggcaccaa ggggaaggtg
      601 cgcctcccgg ccctgcacat cgcggcccgc aacgacgaca cgcgcacggc tgcggtgctg
      661 ctgcagaacg accccaaccc ggacgtgctt tccaagacgg gattcacgcc cctgcacatt
      721 gcggctcact acgagaacct caacgtggcc cagttgctcc tcaacagagg agccagcgtc
      781 aatttcacac cacagaacgg catcacgcca ctgcacatcg cctcccgcag gggcaacgtg
      841 atcatggtgc ggctgctgct ggatcgggga gcccagatag aaaccaagac caaggacgaa
      901 ttgacacctc tccactgtgc agctcgaaat gggcacgtgc gaatctcaga gatcctgctg
      961 gaccacgggg caccaatcca agccaaaacc aagaacggcc tgtccccaat tcacatggcg
     1021 gctcagggag accacctcga ctgtgtccgg ctcctgttgc aatacgacgc agagatagac
     1081 gacatcaccc tggaccacct gaccccactc cacgtggctg cccactgtgg acaccacagg
     1141 gtggctaagg tccttctgga taaaggggcc aaacccaact ccagagccct gaatggcttt
     1201 acccccttac acatcgcctg caaaaagaac cacgtccgtg tcatggagct gctgctgaag
     1261 acgggagcct cgatcgacgc ggtcaccgag tctggcctga cacctctcca cgtggcctcc
     1321 ttcatggggc accttcccat cgtgaagaac ctcctgcagc ggggggcgtc gcccaacgtc
     1381 tccaacgtga aagtggagac cccgctacac atggcagcca gagccgggca cacggaagtg
     1441 gccaaatatt tactccagaa caaagccaaa gtcaatgcca aggccaagga tgaccagacc
     1501 ccacttcact gtgcagctcg catcggccac acaaacatgg tgaagctcct gctggaaaat
     1561 aacgccaacc ccaacctggc caccaccgcc gggcacaccc ccctgcacat tgcagcccgt
     1621 gagggccatg tggaaacagt cctggccctt ctggaaaagg aagcatccca ggcctgcatg
     1681 accaagaaag gatttacccc tctgcacgtg gcggccaagt acgggaaggt gcgggtggca
     1741 gagctgctgc tggagcggga cgcacacccg aatgctgccg gaaaaaatgg cctgaccccc
     1801 ctgcacgtgg ccgtccatca caacaacctg gacatcgtca agctgctgct tccccggggc
     1861 ggctccccgc acagccctgc ctggaatggc tacacccctt tgcacatcgc tgccaagcag
     1921 aaccaggtgg aggtggcccg tagtctgctg cagtatgggg gctcagcaaa cgccgagtcg
     1981 gtgcaaggtg tgacgcccct tcacctggcc gcccaggagg gccacgcaga gatggtggct
     2041 ctgctgctct cgaaacaagc caatggcaac ctggggaaca agagcggact cactcccctc
     2101 catctggtag cacaagaagg ccacgttcca gtggcagatg tgctgatcaa acacggcgtc
     2161 atggtggacg ccaccacccg gatgggctac actcccctcc atgtggccag tcactatgga
     2221 aacatcaagc tggtgaagtt tctgctgcag caccaggcag atgtcaatgc caagaccaag
     2281 ctaggataca gccccctgca ccaggcagcc cagcagggac acacagacat cgtgactctg
     2341 cttctgaaaa acggtgcttc cccaaacgag gtcagctcgg atggaaccac acctctggcc
     2401 atagccaagc gcttgggcta catttctgtc accgacgtgc tcaaggtcgt cacggatgaa
     2461 accagtttcg tgttagtcag tgataagcat cgaatgagtt tccctgagac agttgatgag
     2521 atcctggatg tctcggaaga tgaaggggaa gaactcatca gcttcaaggc tgagaggcgg
     2581 gattccaggg atgttgatga agagaaggag ctgctggatt ttgtgccgaa gctagaccaa
     2641 gtggtggaat ctccagccat ccccaggatt ccctgtgcca tgcctgagac agtggtgatc
     2701 aggtcagaag agcaggagca ggcatctaaa gagtatgatg aggactccct catccccagc
     2761 agcccggcca ccgagacctc agacaacatc agcccggtgg ccagcccggt gcatacaggg
     2821 tttctggtga gcttcatggt tgacgcccgg ggtggttcca tgagaggaag tcgccacaac
     2881 ggcctgcgag tggtgatccc gccacggacg tgcgcagcgc ccacccgcat cacctgccgc
     2941 ctggtcaagc cccagaagct cagcacgccg cccccactgg ccgaggagga gggcctggcc
     3001 agcaggatca tagcactggg gcccacgggg gcacagttcc tgagccctgt aatcgtggag
     3061 atcccgcact ttgcctccca tggccgtgga gaccgcgagc tcgtggttct gaggagcgaa
     3121 aacggctccg tgtggaagga gcacaggagc cgctatggag agagctacct ggatcagatc
     3181 ctcaacggga tggacgaaga gctggggagc ctggaggagc tagagaagaa gagggtgtgc
     3241 cgaatcatca ccaccgactt cccgctgtac ttcgtgatca tgtcacggct ctgccaggac
     3301 tacgacacca tcggtcccga agggggctcc ctgaagagca agctggtgcc cctggtacag
     3361 gcaacgttcc cggagaatgc cgtcaccaag agagtgaagc tggctctgca ggcccagcct
     3421 gtcccggatg agcttgtcac taagctcctg ggcaaccagg ccacattcag ccccattgtc
     3481 accgtggagc cccggcgccg gaagttccac cgccccattg ggcttcggat cccactacct
     3541 ccttcctgga ccgacaaccc gagggacagc ggggagggag acaccaccag cctgcgcctg
     3601 ctttgcagcg tcattggagg aacagaccaa gcccagtggg aagacataac aggaaccacc
     3661 aaacttgtat atgccaacga gtgcgccaac ttcaccacca atgtctctgc caggttttgg
     3721 ctgtcggact gtcctcggac tgctgaggct gtgaactttg ccaccctgct gtacaaagag
     3781 ctcactgcag tgccctacat ggccaaattc gtcatctttg ccaagatgaa tgacccccga
     3841 gaggggcgcc tgcgctgcta ctgcatgaca gatgataaag tggacaagac cctggagcag
     3901 catgagaact tcgtggaggt ggcccggagc agggacatag aggtgttgga aggaatgtcc
     3961 ctgtttgcag aactctctgg gaacctggtg cctgtgaaga aagctgccca gcagcggagc
     4021 ttccacttcc agtcatttcg ggagaaccgt ctggccatgc ctgtaaaggt gagggacagc
     4081 agtcgagagc cgggagggtc cctgtcgttt ctgcgcaagg cgatgaagta cgaggacacc
     4141 cagcacattc tctgccacct gaacatcacc atgcccccct gcgccaaggg aagtggagcc
     4201 gaagatagga gaaggacccc gacgcccctg gccctgcgat acagcattct cagtgagtcc
     4261 acaccaggtt ctctcagtgg gacagagcag gcagagatga agatggctgt tatctcagag
     4321 cacctcggtc tcagctgggc agagttggcc cgggagctgc agttcagtgt ggaagacatc
     4381 aacaggatcc gagtggaaaa tcccaactcc ctgttggagc agagtgtggc cttgctgaac
     4441 ctctgggtca tccgtgaagg ccaaaacgca aacatggaga atctgtacac agccctgcag
     4501 agcattgacc gtggcgagat cgtgaacatg ctggagggtt ccggccgaca gagccgcaac
     4561 ttgaagccag acaggcggca caccgaccgc gactactcgc tgtcaccctc ccagatgaat
     4621 ggttactcct cactgcagga cgagctgctg tcccctgcct ccctgggctg tgcactttcc
     4681 tctccgctac gtgcagacca gtactggaat gaggtggccg tcctagacgc catccccttg
     4741 gcggccacgg agcatgacac catgctggag atgtctgaca tgcaggtgtg gtctgcgggc
     4801 ctcacgcctt ctctggtcac tgctgaggac tcctctctgg agtgtagcaa ggctgaggac
     4861 tctgatgcca caggtcacga gtggaagttg gagggggcac tctcagagga accgcggggc
     4921 cccgagttgg gctctctgga acttgtggag gacgacacag tggattcaga tgccacaaat
     4981 ggccttatcg atttgcttga acaggaggaa ggtcagaggt cagaagagaa gctgccaggt
     5041 tctaagaggc aggatgacgc gacaggtgca gggcaggact cagagaatga agtgtctctt
     5101 gtttcaggcc atcagagggg gcaagcccga atcacacatt cccccaccgt gagtcaggtg
     5161 acggagagga gtcaggacag actgcaggac tgggatgcag acggctcgat tgtctcatac
     5221 ctgcaagatg ctgcacaagg ttcctggcaa gaggaggtca cgcaaggtcc acactcattc
     5281 cagggaacaa gtaccatgac tgaagggcta gagcccggtg gatctcagga gtacgagaag
     5341 gtcctggtgt ctgtaagtga gcacacgtgg acagaacagc ccgaggctga gagctcccag
     5401 gccgacaggg accggaggca gcaaggccaa gaagagcagg tgcaggaggc caagaacacc
     5461 ttcacccaag tggtgcaggg gaatgagttt cagaatattc caggggagca ggtgacagag
     5521 gagcaattca cggatgagca gggcaacatt gtcaccaaga agatcattcg caaggtggtt
     5581 cgacagatag acttgtccag cgccgatgcc gcccaggagc acgaggaggt ggagctgaga
     5641 gggagtggcc tacagccgga cctgatagag ggcaggaagg gggcgcagat agtgaagcgg
     5701 gccagcctga aaagggggaa acagtgaccc cgagccgctc tccttggagt agcctctcgg
     5761 gaggatcaca cctcgacacc caacccctga accccacaca ctctgccatg cacacaggag
     5821 gagagctgga cctgagggcc accgcagcgg tgcacacatt cctctgggct gacggcatga
     5881 cctctgtaag ggactcctgc tagtcccctc ttggcatgaa tgactgactg tagacgcatg
     5941 acctccaggc ttcaatcctg cctcttgcaa tgacagctga tctgtcggaa ccaggacaca
     6001 aaagcagcaa gaagcgggga gagagaggga tagaaaacaa gcgcaggaga gcctgcgaac
     6061 gcaaaagtga atgagggctt tttgtggctg gggatgggtt ttggttttgg ggtttttttt
     6121 ttaaattgtt ttgacttcgt acagggtact ttttcccaac ctcatctgtc agaaatccat
     6181 gtgggcttcc tggaaagaaa aaaaaaaaaa agaaaactag gcatgaaatc agtttaacac
     6241 cttaatctta agccatgtcc tcatctgccc accctcccca acccacatac ctccattcca
     6301 cttgtgacac cccctcgact ccctggtgac gctcctccca gatcgctcta catgacattc
     6361 aggacacaca cacacacaca cacacaccac tcgcctccac tgaatctaca cacagatttt
     6421 actgtgactt ctgaagctgt atagactctg ctgtggataa attggaattt tttatgttgt
     6481 ctctctctct gccaatttca atacgaatca tcttccaatg gaaaatcatt accttgaaga
     6541 gtgcattcgg ggttccttgt gttagggact taagaatctg aggcgaggac ccccaggctt
     6601 acctgtagga ctcaaaggga gccaggaccc catctgaaag ggtctcctct ctcagttggg
     6661 ggacaggccg gtggctgacc caggattgca ccagcatgtc catagagaag aggttttcta
     6721 tgtcttcaag cactatatca tagtccgtgt tcaaagtgta aactgtacag taatcagcct
     6781 tgtgtatatg aaaaacaata aatactatgc aaaccaatag aaacatttag cagtacgtac
     6841 agagcctgac agcgcagctc ctgaggactt ctgcggctgc aggagaagga gctgtggcct
     6901 gtctttcagt gaaagaggaa ggaaaggagt agggctagtg aatgtatctg ccaggtctta
     6961 gaagtcagga ccacccaggg tctcccagag tgaacttggt gctctaagtc caggcagcct
     7021 gcaggctggg gaccctgggt gcaggtgcca ggctcgaggc tgttccctgt cctgcctcag
     7081 tgcctcccct ctgcccagcc cctgtggcct cttggagagg cgagccaggg ggctccccgg
     7141 accgcatggc cggtgagagt agagtcggag gcggagagcc tggacgccca gaggccgggg
     7201 ccaggctctg ggtcaaggca ggaaggagac agacagctgg cagttcctcg cctcggtgcc
     7261 atcagggagg agccttaagt tccactagag cctccggccc agggaggcca gaatcagcac
     7321 aatcccggcc ccgccctcgc ggcgggcgcc cccagggcca ggagagagag acagggctgg
     7381 ctcctctcgc ccctggagcc gcggtgtgga cggcccggcc ccgtccgccc cgccgctctg
     7441 cgggagtgtc ctcgggacac acttaaacct tccaagtagc acagaagccc cctccacgtc
     7501 agaggccccc tggccgcggg gcctgtgagc ggagaggggc cctgcttccc acacaaatcc
     7561 gccccggtgc acccccggcc aggcaggccc cggggcggcc taggcgcctt gtcggccggg
     7621 ggtggggctg cttttgcttc ctttagaggg gcgggcaggg agaggaagct ctagctccgg
     7681 gcctgagttt ccccgaggct gcgtcggagg agctgcaggg cgagcccgga cggctgccgt
     7741 ctccgccaga ggccaccccg gcccggcctc cagggcgcag aggcgccccc ctccatcctc
     7801 ccccatcccg ggggccacgg caggggggcc gcacagcgcg gctgggaccg attcggtgac
     7861 ggcccaagga agctgggctc agggctcgcc tctcccgcct cgatcctgca ccttcctctc
     7921 ccaggtcgct gctgcagcca acccagtagc ccccttagcg cccccgcgga ccccgcagct
     7981 ccagactccg ctttggcacc tctgctccgg ctggcatggc tgcacgctcc ggctgctagt
     8041 aggagccgtt aggggaggag tttggggtct ccacacgatg cctagagaat gctgcagtct
     8101 gcacattaga cgctttttag aagttttgaa attaccttga tttttttaat tgttatgaaa
     8161 atggatcttt tcttgactct cccacatgct ctgttatggg agagaatccc ctaccctact
     8221 ctgatgtata gaccattctc ccttcaccag ccgaaccaat gtcaaaatta ataaagaaat
     8281 ggactaatgg ca
//
