LOCUS       NM_015985               4692 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens angiopoietin 4 (ANGPT4), transcript variant 1, mRNA.
ACCESSION   NM_015985
VERSION     NM_015985.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Jeong,S.Y., Kang,M.L., Park,J.W. and Im,G.I.
  TITLE     Dual functional nanoparticles containing SOX duo and ANGPT4 shRNA
            for osteoarthritis treatment
  JOURNAL   J Biomed Mater Res B Appl Biomater 108 (1), 234-242 (2020)
   PUBMED   30957437
  REMARK    GeneRIF: Dual functional nanoparticles containing SOX duo and
            ANGPT4 shRNA for osteoarthritis treatment.
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Kesler,C.T., Pereira,E.R., Cui,C.H., Nelson,G.M., Masuck,D.J.,
            Baish,J.W. and Padera,T.P.
  TITLE     Angiopoietin-4 increases permeability of blood vessels and promotes
            lymphatic dilation
  JOURNAL   FASEB J 29 (9), 3668-3677 (2015)
   PUBMED   25977256
  REMARK    GeneRIF: Ang-4 also promoted survival of LECs. Thus, blocking Ang-4
            may prune the draining lymphatic vasculature and decrease
            interstitial fluid pressure (IFP) by reducing vascular
            permeability.
REFERENCE   3  (bases 1 to 4692)
  AUTHORS   Frenzel,E., Wrenger,S., Immenschuh,S., Koczulla,R., Mahadeva,R.,
            Deeg,H.J., Dinarello,C.A., Welte,T., Marcondes,A.M. and
            Janciauskiene,S.
  TITLE     Acute-phase protein alpha1-antitrypsin--a novel regulator of
            angiopoietin-like protein 4 transcription and secretion
  JOURNAL   J Immunol 192 (11), 5354-5362 (2014)
   PUBMED   24760148
  REMARK    GeneRIF: acute-phase protein A1AT is a physiological regulator of
            angptl4, another acute-phase protein.
REFERENCE   4  (bases 1 to 4692)
  AUTHORS   Gray,N.E., Lam,L.N., Yang,K., Zhou,A.Y., Koliwad,S. and Wang,J.C.
  TITLE     Angiopoietin-like 4 (Angptl4) protein is a physiological mediator
            of intracellular lipolysis in murine adipocytes
  JOURNAL   J Biol Chem 287 (11), 8444-8456 (2012)
   PUBMED   22267746
  REMARK    GeneRIF: Angptl4 is a glucocorticoid-responsive mediator of
            fasting-induced intracellular lipolysis and stimulates cAMP
            signaling in adipocytes
            Erratum:[J Biol Chem. 2017 Sep 29;292(39):16135. doi:
            10.1074/jbc.A111.294124.. PMID: 28963337]
REFERENCE   5  (bases 1 to 4692)
  AUTHORS   Andersen,S., Donnem,T., Al-Shibli,K., Al-Saad,S., Stenvold,H.,
            Busund,L.T. and Bremnes,R.M.
  TITLE     Prognostic impacts of angiopoietins in NSCLC tumor cells and
            stroma: VEGF-A impact is strongly associated with Ang-2
  JOURNAL   PLoS One 6 (5), e19773 (2011)
   PUBMED   21603628
  REMARK    GeneRIF: In tumor cells, only Ang-4 expression has prognostic
            impact in NSCLC. In NSCLC tumor stroma, Ang-4 and Ang-2 are
            independently associated with survival.
REFERENCE   6  (bases 1 to 4692)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
REFERENCE   7  (bases 1 to 4692)
  AUTHORS   Nishimura,M., Miki,T., Yashima,R., Yokoi,N., Yano,H., Sato,Y. and
            Seino,S.
  TITLE     Angiopoietin-3, a novel member of the angiopoietin family
  JOURNAL   FEBS Lett 448 (2-3), 254-256 (1999)
   PUBMED   10218486
REFERENCE   8  (bases 1 to 4692)
  AUTHORS   Valenzuela,D.M., Griffiths,J.A., Rojas,J., Aldrich,T.H.,
            Jones,P.F., Zhou,H., McClain,J., Copeland,N.G., Gilbert,D.J.,
            Jenkins,N.A., Huang,T., Papadopoulos,N., Maisonpierre,P.C.,
            Davis,S. and Yancopoulos,G.D.
  TITLE     Angiopoietins 3 and 4: diverging gene counterparts in mice and
            humans
  JOURNAL   Proc Natl Acad Sci U S A 96 (5), 1904-1909 (1999)
   PUBMED   10051567
REFERENCE   9  (bases 1 to 4692)
  AUTHORS   Kim,I., Kwak,H.J., Ahn,J.E., So,J.N., Liu,M., Koh,K.N. and Koh,G.Y.
  TITLE     Molecular cloning and characterization of a novel angiopoietin
            family protein, angiopoietin-3
  JOURNAL   FEBS Lett 443 (3), 353-356 (1999)
   PUBMED   10025962
REFERENCE   10 (bases 1 to 4692)
  AUTHORS   Grosios,K., Leek,J.P., Markham,A.F., Yancopoulos,G.D. and
            Jones,P.F.
  TITLE     Assignment of ANGPT4, ANGPT1, and ANGPT2 encoding angiopoietins 4,
            1 and 2 to human chromosome bands 20p13, 8q22.3-->q23 and 8p23.1,
            respectively, by in situ hybridization and radiation hybrid mapping
  JOURNAL   Cytogenet Cell Genet 84 (1-2), 118-120 (1999)
   PUBMED   10343124
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK304756.1, AF074332.1,
            AL161939.10 and BM999095.1.
            
            On Nov 22, 2018 this sequence version replaced NM_015985.3.
            
            Summary: Angiopoietins are proteins with important roles in
            vascular development and angiogenesis. All angiopoietins bind with
            similar affinity to an endothelial cell-specific tyrosine-protein
            kinase receptor. The mechanism by which they contribute to
            angiogenesis is thought to involve regulation of endothelial cell
            interactions with supporting perivascular cells. The protein
            encoded by this gene functions as an agonist and is an
            angiopoietin. [provided by RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF074332.1, SRR1803611.235771.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145245, SAMEA2156099
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000381922.5/ ENSP00000371347.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1032              AK304756.1         1-1032
            1033-1511           AF074332.1         1018-1496
            1512-1799           AK304756.1         1381-1668
            1800-4185           AL161939.10        13687-16072         c
            4186-4692           BM999095.1         20-526              c
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p13"
     gene            1..4692
                     /gene="ANGPT4"
                     /gene_synonym="ANG3; ANG4"
                     /note="angiopoietin 4"
                     /db_xref="GeneID:51378"
                     /db_xref="HGNC:HGNC:487"
                     /db_xref="MIM:603705"
     exon            1..429
                     /gene="ANGPT4"
                     /gene_synonym="ANG3; ANG4"
                     /inference="alignment:Splign:2.1.0"
     CDS             121..1632
                     /gene="ANGPT4"
                     /gene_synonym="ANG3; ANG4"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1"
                     /codon_start=1
                     /product="angiopoietin-4 isoform 1 precursor"
                     /protein_id="NP_057069.1"
                     /db_xref="CCDS:CCDS13009.1"
                     /db_xref="GeneID:51378"
                     /db_xref="HGNC:HGNC:487"
                     /db_xref="MIM:603705"
                     /translation="MLSQLAMLQGSLLLVVATMSVAQQTRQEADRGCETLVVQHGHCS
                     YTFLLPKSEPCPPGPEVSRDSNTLQRESLANPLHLGKLPTQQVKQLEQALQNNTQWLK
                     KLERAIKTILRSKLEQVQQQMAQNQTAPMLELGTSLLNQTTAQIRKLTDMEAQLLNQT
                     SRMDAQMPETFLSTNKLENQLLLQRQKLQQLQGQNSALEKRLQALETKQQEELASILS
                     KKAKLLNTLSRQSAALTNIERGLRGVRHNSSLLQDQQHSLRQLLVLLRHLVQERANAS
                     APAFIMAGEQVFQDCAEIQRSGASASGVYTIQVSNATKPRKVFCDLQSSGGRWTLIQR
                     RENGTVNFQRNWKDYKQGFGDPAGEHWLGNEVVHQLTRRAAYSLRVELQDWEGHEAYA
                     QYEHFHLGSENQLYRLSVVGYSGSAGRQSSLVLQNTSFSTLDSDNDHCLCKCAQVMSG
                     GWWFDACGLSNLNGVYYHAPDNKYKMDGIRWHYFKGPSYSLRASRMMIRPLDI"
     exon            430..585
                     /gene="ANGPT4"
                     /gene_synonym="ANG3; ANG4"
                     /inference="alignment:Splign:2.1.0"
     exon            586..707
                     /gene="ANGPT4"
                     /gene_synonym="ANG3; ANG4"
                     /inference="alignment:Splign:2.1.0"
     exon            708..955
                     /gene="ANGPT4"
                     /gene_synonym="ANG3; ANG4"
                     /inference="alignment:Splign:2.1.0"
     exon            956..1071
                     /gene="ANGPT4"
                     /gene_synonym="ANG3; ANG4"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..1173
                     /gene="ANGPT4"
                     /gene_synonym="ANG3; ANG4"
                     /inference="alignment:Splign:2.1.0"
     exon            1174..1340
                     /gene="ANGPT4"
                     /gene_synonym="ANG3; ANG4"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1471
                     /gene="ANGPT4"
                     /gene_synonym="ANG3; ANG4"
                     /inference="alignment:Splign:2.1.0"
     exon            1472..4692
                     /gene="ANGPT4"
                     /gene_synonym="ANG3; ANG4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3318..3323
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANGPT4"
                     /gene_synonym="ANG3; ANG4"
                     /note="hexamer: AGTAAA"
     polyA_site      3338
                     /gene="ANGPT4"
                     /gene_synonym="ANG3; ANG4"
     regulatory      4667..4672
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANGPT4"
                     /gene_synonym="ANG3; ANG4"
                     /note="hexamer: AGTAAA"
     polyA_site      4692
                     /gene="ANGPT4"
                     /gene_synonym="ANG3; ANG4"
                     /note="major polyA site"
ORIGIN      
        1 agacagaggt ttgtagctgc agctgcaggc aagcctggcc actgttggct gcagcaggac
       61 atcccaggca cagcccctag ggctctgagc agacatccct cgccattgac acatcttcag
      121 atgctctccc agctagccat gctgcagggc agcctcctcc ttgtggttgc caccatgtct
      181 gtggctcaac agacaaggca ggaggcggat aggggctgcg agacacttgt agtccagcac
      241 ggccactgta gctacacctt cttgctgccc aagtctgagc cctgccctcc ggggcctgag
      301 gtctccaggg actccaacac cctccagaga gaatcactgg ccaacccact gcacctgggg
      361 aagttgccca cccagcaggt gaaacagctg gagcaggcac tgcagaacaa cacgcagtgg
      421 ctgaagaagc tagagagggc catcaagacg atcttgaggt cgaagctgga gcaggtccag
      481 cagcaaatgg cccagaatca gacggccccc atgctagagc tgggcaccag cctcctgaac
      541 cagaccactg cccagatccg caagctgacc gacatggagg ctcagctcct gaaccagaca
      601 tcaagaatgg atgcccagat gccagagacc tttctgtcca ccaacaagct ggagaaccag
      661 ctgctgctac agaggcagaa gctccagcag cttcagggcc aaaacagcgc gctcgagaag
      721 cggttgcagg ccctggagac caagcagcag gaggagctgg ccagcatcct cagcaagaag
      781 gcgaagctgc tgaacacgct gagccgccag agcgccgccc tcaccaacat cgagcgcggc
      841 ctgcgcggtg tcaggcacaa ctccagcctc ctgcaggacc agcagcacag cctgcgccag
      901 ctgctggtgt tgttgcggca cctggtgcaa gaaagggcta acgcctcggc cccggccttc
      961 ataatggcag gtgagcaggt gttccaggac tgtgcagaga tccagcgctc tggggccagt
     1021 gccagtggtg tctacaccat ccaggtgtcc aatgcaacga agcccaggaa ggtgttctgt
     1081 gacctgcaga gcagtggagg caggtggacc ctcatccagc gccgtgagaa tggcaccgtg
     1141 aattttcagc ggaactggaa ggattacaaa cagggcttcg gagacccagc tggggagcac
     1201 tggctgggca atgaagtggt gcaccagctc accagaaggg cagcctactc tctgcgtgtg
     1261 gagctgcaag actgggaagg ccacgaggcc tatgcccagt acgaacattt ccacctgggc
     1321 agtgagaacc agctatacag gctttctgtg gtcgggtaca gcggctcagc agggcgccag
     1381 agcagcctgg tcctgcagaa caccagcttt agcacccttg actcagacaa cgaccactgt
     1441 ctctgcaagt gtgcccaagt gatgtctgga gggtggtggt ttgacgcctg tggcctgtca
     1501 aacctcaacg gcgtctacta ccacgctccc gacaacaagt acaagatgga cggcatccgc
     1561 tggcactact tcaagggccc cagctactca ctgcgtgcct ctcgcatgat gatacggcct
     1621 ttggacatct aacgagcagc tgtgccagag gctggaccac acaggagaag ctcggacttg
     1681 gcactcctgg acaacctgga cccagatgca agacactgtg ccaccgcctt ccctgacacc
     1741 ctgggcttcc tgagccagcc ctccttgacc cagaagtcca gaagggtcat ctgcccccca
     1801 actcccctcc gtctgtgaca tggagggtgt tcggggccca tccctctgat gtagtcctcg
     1861 cccctcttct ctccctcccc cttcaggggc tccctgcctg agggtcacag taccttgaat
     1921 gggctgagaa cagaccaaac ttgattccca tgaccaatgg tggggttgca ggcaggtggg
     1981 aatgtatttg cacatcggaa gctgcccaga tggcccaggt tctctccctt ggattggcaa
     2041 gaaggccatc tcccattcta agctcctgtt ccaagatttt ctagtcttga gatgtccttg
     2101 aactttcttt tcaagtctga aggggctgca tccacccctt agtgggtggg ttaatcatta
     2161 tttccccttc acacttcacc acttctaggt tctaatgacc ctagatctca gggtctttag
     2221 acttcaccac ttctaggctt taccacttca ccacttctag gctccaatgt ttggagctca
     2281 gggtctttag gagacccaaa aggacatgct ccttcacctc cagcatgtcc tagaggatgt
     2341 gtcacaggga ataactatgg cttgtctcta aaagtaccta tgagcaatga gaaaaggaaa
     2401 cagcaggtta agtcaaagtg aacaggcact cttcactgca ggactgatca gagcctttaa
     2461 tatggccaag tgccttgtga ctacccatga aggggctaga gtgggcagct ttctccaaat
     2521 ttacttattt gaaaatgggc tcggtttgtc ccagagcatc tcacaggact gtagatgctc
     2581 ttggacaaag ctagtgctcc cctggcataa ggaggagccc tacgacccca tccccacccc
     2641 agctatactc accctttttg gctacaaggg ccacagtgac agcctcaaac aacctctaaa
     2701 aacaactgga aataaccttt cagttaaaac agataccatc cctgaagaag ggtctagaac
     2761 taggtccctg tctgtgttat aggctcatgt cctccaaggc tccttcaagt cccaggaagc
     2821 tgatctctac ctgggtggct tcccttagga ctccctgtaa cctcaactcc cccaggctca
     2881 attacaggga ctgttaggca ggacatctgt ctccaagtcc agatcctctc tgcctccaag
     2941 ccctaacccc tagcctccct cccttcccca tccagcagtg atgctgcctc tgtggtggta
     3001 ggtggggagc tgcaggggag gagataaggc ctctgcctga gtttgggaga ccagggccct
     3061 catagcttct ttcagaggat ggagtcagaa aggatccaca gctactctgt cacctgcccc
     3121 catcactgtg tcatgctgtc tgccctgttg tcatcagcca acacccaggc atagccagga
     3181 gcccacctgc cctaccgcca ggatacacct ctgtcctcag aaggttttct cctggatgag
     3241 actgagccaa tgggaatggg accccttcat ccccctggct cgccccagcc ctgagtccca
     3301 ctctcagccg atccctgagt aaacccagca cagactgact ttgatctcat tcctgggaat
     3361 tagcactctt ccccttcaag actcaaagga catggttgct aatggtggca tttcaggcat
     3421 gatgggaaat ctttaggggc agattgctgc ccagagagct caaatcgcct taagcagcat
     3481 ttgcccagca gacctttatt tagcctctac tgtgtgcagt gtggtgtggt gggcagggct
     3541 ttggagtcgg acaaacctgc tccagctctg acactttggt ccagtggctc agcctctcaa
     3601 ggcaccagtt atcttcacat catcaaagcc tcagttttcc catctgtaaa atggagatga
     3661 taatattcct tcctggctgg gctatggcaa ggaggaaatg agaccatgta tgtcatcttc
     3721 ttaatagagc ctggcatgaa gcaggtgcct aataaatgtt tgtcctcaaa gaggagaatg
     3781 gggtgaggaa ggcattcccc agcacatgcc gccccttctc ctgcactcag gtgaggaaaa
     3841 ggcattttat ttttgtatcc acatcattta tttttctatt gtagtttcta ggctgactgc
     3901 aagctagaga ggagacaggg caaagctgtg aggcccaggg acagaactcc tctgggtggg
     3961 ttgaaggccc aagtccctct ctactcccat tttataaggg ggcaggaagc tgatttgagt
     4021 tatcctcaga cacctgttct ttatgtaatt ttattttatt tttttgagac agagtctcac
     4081 tctgtcaccc aggctggagt gcagtggcat gatctcagat cactgcaatc tctgcctcct
     4141 ggttcaagtg attctcctac ctcagcctcc tgagtaatgg gattacagac gcctaccacc
     4201 acgcccggaa aacttttgta tttttagtag aaacgggttt tcaccatgtt ggccaggctg
     4261 gtctcaaact cctggcctca tgtgatccac ctgcctcagc ctcccaaagt gctgggatta
     4321 caggcatgag ccaccatacc cagcctcaga cacctgttct taaatattca tccttctttc
     4381 ttaccttcct tcctcttcca tgccaggact caggtataag ggatagaaat tctagcccta
     4441 aggaataaat tgactcacat aactggaaag tctaagggta aaggcaagtg aggttagatc
     4501 cagaggctca aatgatgtca gctccacctc tcagcccctc catctgcccc gttgacttca
     4561 ttctcagcca ggatctttcc tcacaagaag gctctggcag ccccaggctc atgtcctccc
     4621 agctcagcat ccctgacccg gggagctccc tcgtctccat gattccagta aaggaatgat
     4681 tttctgcagc ca
//
