LOCUS       NM_016237               2574 bp    mRNA    linear   PRI 03-MAY-2024
DEFINITION  Homo sapiens anaphase promoting complex subunit 5 (ANAPC5),
            transcript variant 1, mRNA.
ACCESSION   NM_016237
VERSION     NM_016237.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Yau,R.G., Doerner,K., Castellanos,E.R., Haakonsen,D.L., Werner,A.,
            Wang,N., Yang,X.W., Martinez-Martin,N., Matsumoto,M.L., Dixit,V.M.
            and Rape,M.
  TITLE     Assembly and Function of Heterotypic Ubiquitin Chains in Cell-Cycle
            and Protein Quality Control
  JOURNAL   Cell 171 (4), 918-933 (2017)
   PUBMED   29033132
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Xu,M., Liu,X., Xu,Y., Zhu,S. and Gao,Y.
  TITLE     Co-expression of Axin and APC gene fragments inhibits colorectal
            cancer cell growth via regulation of the Wnt signaling pathway
  JOURNAL   Mol Med Rep 16 (4), 3783-3790 (2017)
   PUBMED   28731177
  REMARK    GeneRIF: coexpression of APC5 and Axin genes significantly
            downregulated Wnt signaling in human SW480 CRC cells and inhibited
            cell growth.
REFERENCE   3  (bases 1 to 2574)
  AUTHORS   Zhang,S., Chang,L., Alfieri,C., Zhang,Z., Yang,J., Maslen,S.,
            Skehel,M. and Barford,D.
  TITLE     Molecular mechanism of APC/C activation by mitotic phosphorylation
  JOURNAL   Nature 533 (7602), 260-264 (2016)
   PUBMED   27120157
REFERENCE   4  (bases 1 to 2574)
  AUTHORS   Song,M.S., Carracedo,A., Salmena,L., Song,S.J., Egia,A.,
            Malumbres,M. and Pandolfi,P.P.
  TITLE     Nuclear PTEN regulates the APC-CDH1 tumor-suppressive complex in a
            phosphatase-independent manner
  JOURNAL   Cell 144 (2), 187-199 (2011)
   PUBMED   21241890
REFERENCE   5  (bases 1 to 2574)
  AUTHORS   Tran,K., Kamil,J.P., Coen,D.M. and Spector,D.H.
  TITLE     Inactivation and disassembly of the anaphase-promoting complex
            during human cytomegalovirus infection is associated with
            degradation of the APC5 and APC4 subunits and does not require
            UL97-mediated phosphorylation of Cdh1
  JOURNAL   J Virol 84 (20), 10832-10843 (2010)
   PUBMED   20686030
  REMARK    GeneRIF: Inactivation and disassembly of the anaphase-promoting
            complex during human cytomegalovirus infection is associated with
            degradation of the APC5 and APC4 subunits and does not require
            UL97-mediated phosphorylation of Cdh1.
REFERENCE   6  (bases 1 to 2574)
  AUTHORS   Kraft,C., Herzog,F., Gieffers,C., Mechtler,K., Hagting,A., Pines,J.
            and Peters,J.M.
  TITLE     Mitotic regulation of the human anaphase-promoting complex by
            phosphorylation
  JOURNAL   EMBO J 22 (24), 6598-6609 (2003)
   PUBMED   14657031
REFERENCE   7  (bases 1 to 2574)
  AUTHORS   Vodermaier,H.C., Gieffers,C., Maurer-Stroh,S., Eisenhaber,F. and
            Peters,J.M.
  TITLE     TPR subunits of the anaphase-promoting complex mediate binding to
            the activator protein CDH1
  JOURNAL   Curr Biol 13 (17), 1459-1468 (2003)
   PUBMED   12956947
REFERENCE   8  (bases 1 to 2574)
  AUTHORS   Autieri,M.V.
  TITLE     Expression of anaphase-promoting complex 5 in balloon
            angioplasty-injured rat carotid arteries and mitogen-stimulated
            human vascular smooth muscle cells
  JOURNAL   Biochem Biophys Res Commun 282 (3), 723-728 (2001)
   PUBMED   11401522
REFERENCE   9  (bases 1 to 2574)
  AUTHORS   Grossberger,R., Gieffers,C., Zachariae,W., Podtelejnikov,A.V.,
            Schleiffer,A., Nasmyth,K., Mann,M. and Peters,J.M.
  TITLE     Characterization of the DOC1/APC10 subunit of the yeast and the
            human anaphase-promoting complex
  JOURNAL   J Biol Chem 274 (20), 14500-14507 (1999)
   PUBMED   10318877
REFERENCE   10 (bases 1 to 2574)
  AUTHORS   Yu,H., Peters,J.M., King,R.W., Page,A.M., Hieter,P. and
            Kirschner,M.W.
  TITLE     Identification of a cullin homology region in a subunit of the
            anaphase-promoting complex
  JOURNAL   Science 279 (5354), 1219-1222 (1998)
   PUBMED   9469815
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AI879332.1, BC001950.1,
            AA349724.1 and BX381618.2.
            
            On Nov 23, 2018 this sequence version replaced NM_016237.4.
            
            Summary: This gene encodes a tetratricopeptide repeat-containing
            component of the anaphase promoting complex/cyclosome (APC/C), a
            large E3 ubiquitin ligase that controls cell cycle progression by
            targeting a number of cell cycle regulators such as B-type cyclins
            for 26S proteasome-mediated degradation through ubiquitination. The
            encoded protein is required for the proper ubiquitination function
            of APC/C and for the interaction of APC/C with transcription
            coactivators. It also interacts with polyA binding protein and
            represses internal ribosome entry site-mediated translation.
            Multiple transcript variants encoding different isoforms have been
            found for this gene. These differences cause translation initiation
            at a downstream AUG and result in a shorter protein (isoform b),
            compared to isoform a. [provided by RefSeq, Nov 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.4105352.1, BC001081.2
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261819.8/ ENSP00000261819.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                AI879332.1         54-69
            17-2462             BC001950.1         1-2446
            2463-2498           AA349724.1         75-110              c
            2499-2574           BX381618.2         433-508
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.31"
     gene            1..2574
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
                     /note="anaphase promoting complex subunit 5"
                     /db_xref="GeneID:51433"
                     /db_xref="HGNC:HGNC:15713"
                     /db_xref="MIM:606948"
     exon            1..278
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
                     /inference="alignment:Splign:2.1.0"
     CDS             72..2339
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
                     /note="isoform a is encoded by transcript variant 1;
                     cyclosome subunit 5"
                     /codon_start=1
                     /product="anaphase-promoting complex subunit 5 isoform a"
                     /protein_id="NP_057321.2"
                     /db_xref="CCDS:CCDS9220.1"
                     /db_xref="GeneID:51433"
                     /db_xref="HGNC:HGNC:15713"
                     /db_xref="MIM:606948"
                     /translation="MASVHESLYFNPMMTNGVVHANVFGIKDWVTPYKIAVLVLLNEM
                     SRTGEGAVSLMERRRLNQLLLPLLQGPDITLSKLYKLIEESCPQLANSVQIRIKLMAE
                     GELKDMEQFFDDLSDSFSGTEPEVHKTSVVGLFLRHMILAYSKLSFSQVFKLYTALQQ
                     YFQNGEKKTVEDADMELTSRDEGERKMEKEELDVSVREEEVSCSGPLSQKQAEFFLSQ
                     QASLLKNDETKALTPASLQKELNNLLKFNPDFAEAHYLSYLNNLRVQDVFSSTHSLLH
                     YFDRLILTGAESKSNGEEGYGRSLRYAALNLAALHCRFGHYQQAELALQEAIRIAQES
                     NDHVCLQHCLSWLYVLGQKRSDSYVLLEHSVKKAVHFGLPYLASLGIQSLVQQRAFAG
                     KTANKLMDALKDSDLLHWKHSLSELIDISIAQKTAIWRLYGRSTMALQQAQMLLSMNS
                     LEAVNAGVQQNNTESFAVALCHLAELHAEQGCFAAASEVLKHLKERFPPNSQHAQLWM
                     LCDQKIQFDRAMNDGKYHLADSLVTGITALNSIEGVYRKAVVLQAQNQMSEAHKLLQK
                     LLVHCQKLKNTEMVISVLLSVAELYWRSSSPTIALPMLLQALALSKEYRLQYLASETV
                     LNLAFAQLILGIPEQALSLLHMAIEPILADGAILDKGRAMFLVAKCQVASAASYDQPK
                     KAEALEAAIENLNEAKNYFAKVDCKERIRDVVYFQARLYHTLGKTQERNRCAMLFRQL
                     HQELPSHGVPLINHL"
     exon            279..358
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
                     /inference="alignment:Splign:2.1.0"
     exon            359..468
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
                     /inference="alignment:Splign:2.1.0"
     exon            469..661
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
                     /inference="alignment:Splign:2.1.0"
     exon            662..728
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
                     /inference="alignment:Splign:2.1.0"
     exon            729..830
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
                     /inference="alignment:Splign:2.1.0"
     exon            831..1021
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..1103
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1193
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..1375
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
                     /inference="alignment:Splign:2.1.0"
     exon            1376..1511
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
                     /inference="alignment:Splign:2.1.0"
     exon            1512..1586
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
                     /inference="alignment:Splign:2.1.0"
     exon            1587..1708
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
                     /inference="alignment:Splign:2.1.0"
     exon            1709..1816
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
                     /inference="alignment:Splign:2.1.0"
     exon            1817..1964
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
                     /inference="alignment:Splign:2.1.0"
     exon            1965..2127
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
                     /inference="alignment:Splign:2.1.0"
     exon            2128..2574
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2443..2448
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
                     /note="hexamer: AATAAA"
     polyA_site      2462
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
                     /note="major polyA site"
     regulatory      2551..2556
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
                     /note="hexamer: AATAAA"
     polyA_site      2574
                     /gene="ANAPC5"
                     /gene_synonym="APC5"
ORIGIN      
        1 ggtagtgttg tgccttgtgg tgacaactgg cggcagcgcg ccgcgggccc gagacttagt
       61 ctcgggccgc catggccagc gtccacgaga gcctctactt caatcccatg atgaccaatg
      121 gggttgtgca cgccaatgtg ttcggcatca aggactgggt gacgccgtac aagatcgcgg
      181 tgctggtgct gctgaacgag atgagccgca caggcgaggg cgccgtcagc ctcatggagc
      241 ggcggaggct caaccagctg ctcctgcccc tgctgcaggg cccagatatt acactgtcaa
      301 aactttacaa gttaattgaa gagtcttgtc cacagctggc aaattcagtg cagatcagaa
      361 tcaaactgat ggctgaaggc gagttgaagg atatggaaca gttttttgat gacctttcag
      421 attctttctc tggaactgaa ccagaggttc acaaaacaag tgtagtaggt ttgtttctgc
      481 gtcacatgat cttggcctac agtaagcttt ctttcagcca agtgtttaaa ctgtacactg
      541 cccttcagca gtacttccag aatggtgaga aaaagacagt ggaggatgct gatatggaac
      601 tgaccagtag agatgagggt gaaagaaaaa tggaaaaaga agaacttgat gtatctgtaa
      661 gagaagagga ggtatcttgc agtgggcctc tgtcccaaaa acaagcagaa ttttttcttt
      721 ctcaacaggc ttctttgcta aagaatgatg agactaaggc cctcactcca gcttccttgc
      781 agaaggaatt aaacaatttg ttgaaattta atcctgattt tgctgaagcg cattatctca
      841 gctacttaaa caacctccgt gtccaagatg ttttcagttc aacacacagt ctcctccatt
      901 attttgatcg tctgattctt accggagccg aaagcaaaag taatggggaa gagggctatg
      961 gccggagctt gagatacgcc gctctgaatc ttgccgccct gcactgccgc ttcggtcact
     1021 atcaacaggc agagctcgcc ctgcaggagg caattaggat tgcccaggag tccaacgatc
     1081 acgtgtgtct ccagcactgt ttgagctggc tttatgtgct ggggcagaag agatccgata
     1141 gctatgttct gctggagcat tctgtgaaga aggcagtaca ttttgggtta ccgtacctcg
     1201 cctccctggg aatacagtcc cttgttcaac agagagcttt tgctgggaag acggcaaaca
     1261 agctgatgga tgccctaaag gactccgacc tcctgcactg gaaacacagc ctgtcagagc
     1321 tcatcgatat cagcatcgca cagaaaacgg ccatctggag gctgtatggc cgcagcacca
     1381 tggcactgca acaggcccag atgttgctga gcatgaacag cctggaggcg gtgaatgcgg
     1441 gcgtgcagca gaacaacaca gagtcctttg ctgtcgcact ctgccacctc gcagagctac
     1501 acgcggagca gggctgtttt gctgcagctt ctgaagtgtt aaagcacttg aaggaacgat
     1561 ttccgcctaa tagtcagcac gcccagttat ggatgctatg tgatcaaaaa atacagtttg
     1621 acagagcaat gaatgatggc aaatatcatt tggctgattc acttgttaca ggaatcacag
     1681 ctctcaatag catagagggt gtttatagga aagcggttgt attacaagct cagaaccaaa
     1741 tgtcagaggc acataagctt ttacaaaaat tgttggttca ttgtcagaaa ctgaagaaca
     1801 cagaaatggt gatcagtgtc ctactgtccg tggcagagct gtactggcga tcttcctccc
     1861 ctaccatcgc gctgcccatg ctcctgcagg ctctggccct ctccaaggag taccggttac
     1921 agtacttggc ctctgaaaca gtgctgaact tggcttttgc gcagctcatt cttggaatcc
     1981 cagaacaggc cttaagtctt ctccacatgg ccatcgagcc catcttggct gacggggcta
     2041 tcctggacaa aggtcgtgcc atgttcttag tggccaagtg ccaggtggct tcagcagctt
     2101 cctacgatca gccgaagaaa gcagaagctc tggaggctgc catcgagaac ctcaatgaag
     2161 ccaagaacta ttttgcaaag gttgactgca aagagcgcat cagggacgtc gtttacttcc
     2221 aggccagact ctaccatacc ctggggaaga cccaggagag gaaccggtgt gcgatgctct
     2281 tccggcagct gcatcaggag ctgccctctc atggggtacc cttgataaac catctctaga
     2341 gaggacatcc ctgctgggct gctgtgcaga gtataagatt ttggacttgt tcatgtcccc
     2401 tctctcccta taaatgatgt atttgtgaca ccctatcttg tcaataaaca gcattctgat
     2461 tagtttgtct tattttgttg ctagtaacta cgtatttgtt ttattcccct tttcttccct
     2521 tttggtagca aaggacacca actttttctt aataaatggg tattttgcta acaa
//
