LOCUS       NM_013367               2635 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens anaphase promoting complex subunit 4 (ANAPC4),
            transcript variant 2, mRNA.
ACCESSION   NM_013367
VERSION     NM_013367.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Wu,H., Zhang,X., Shen,Q., Liu,Y., Gao,Y., Wang,G., Lv,M., Hua,R.,
            Xu,Y., Zhou,P., Wei,Z., Tao,F., He,X., Cao,Y. and Liu,M.
  TITLE     A homozygous loss-of-function mutation in FBXO43 causes human
            non-obstructive azoospermia
  JOURNAL   Clin Genet 101 (1), 55-64 (2022)
   PUBMED   34595750
REFERENCE   3  (bases 1 to 2635)
  AUTHORS   Lee,C.C., Li,B., Yu,H. and Matunis,M.J.
  TITLE     Sumoylation promotes optimal APC/C Activation and Timely Anaphase
  JOURNAL   Elife 7, e29539 (2018)
   PUBMED   29517484
  REMARK    GeneRIF: We have also identified a functionally important SUMO
            interacting motif in the cullin-homology domain of APC2 located
            near the APC4 sumoylation sites and APC/C catalytic core. Our
            findings provide evidence of an important regulatory role for SUMO
            modification and binding in affecting APC/C activation and mitotic
            exit.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2635)
  AUTHORS   Yau,R.G., Doerner,K., Castellanos,E.R., Haakonsen,D.L., Werner,A.,
            Wang,N., Yang,X.W., Martinez-Martin,N., Matsumoto,M.L., Dixit,V.M.
            and Rape,M.
  TITLE     Assembly and Function of Heterotypic Ubiquitin Chains in Cell-Cycle
            and Protein Quality Control
  JOURNAL   Cell 171 (4), 918-933 (2017)
   PUBMED   29033132
REFERENCE   5  (bases 1 to 2635)
  AUTHORS   Zhang,S., Chang,L., Alfieri,C., Zhang,Z., Yang,J., Maslen,S.,
            Skehel,M. and Barford,D.
  TITLE     Molecular mechanism of APC/C activation by mitotic phosphorylation
  JOURNAL   Nature 533 (7602), 260-264 (2016)
   PUBMED   27120157
REFERENCE   6  (bases 1 to 2635)
  AUTHORS   Lukas,C., Sorensen,C.S., Kramer,E., Santoni-Rugiu,E., Lindeneg,C.,
            Peters,J.M., Bartek,J. and Lukas,J.
  TITLE     Accumulation of cyclin B1 requires E2F and cyclin-A-dependent
            rearrangement of the anaphase-promoting complex
  JOURNAL   Nature 401 (6755), 815-818 (1999)
   PUBMED   10548110
REFERENCE   7  (bases 1 to 2635)
  AUTHORS   Gieffers,C., Peters,B.H., Kramer,E.R., Dotti,C.G. and Peters,J.M.
  TITLE     Expression of the CDH1-associated form of the anaphase-promoting
            complex in postmitotic neurons
  JOURNAL   Proc Natl Acad Sci U S A 96 (20), 11317-11322 (1999)
   PUBMED   10500174
REFERENCE   8  (bases 1 to 2635)
  AUTHORS   Grossberger,R., Gieffers,C., Zachariae,W., Podtelejnikov,A.V.,
            Schleiffer,A., Nasmyth,K., Mann,M. and Peters,J.M.
  TITLE     Characterization of the DOC1/APC10 subunit of the yeast and the
            human anaphase-promoting complex
  JOURNAL   J Biol Chem 274 (20), 14500-14507 (1999)
   PUBMED   10318877
REFERENCE   9  (bases 1 to 2635)
  AUTHORS   Yu,H., Peters,J.M., King,R.W., Page,A.M., Hieter,P. and
            Kirschner,M.W.
  TITLE     Identification of a cullin homology region in a subunit of the
            anaphase-promoting complex
  JOURNAL   Science 279 (5354), 1219-1222 (1998)
   PUBMED   9469815
REFERENCE   10 (bases 1 to 2635)
  AUTHORS   Tekolf,W.A., Biddison,W.E., Aster,R.D. and Shaw,S.
  TITLE     Two subgroups of HLA Bw44 defined by cell-mediated lympholysis that
            differ in Bw44 expression on platelets and in patterns of genetic
            linkage disequilibrium
  JOURNAL   J Immunol 129 (4), 1474-1478 (1982)
   PUBMED   6180011
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BI546948.1, BC059383.1 and
            CA312572.1.
            
            On Jun 2, 2019 this sequence version replaced NM_013367.2.
            
            Summary: A large protein complex, termed the anaphase-promoting
            complex (APC), or the cyclosome, promotes metaphase-anaphase
            transition by ubiquitinating its specific substrates such as
            mitotic cyclins and anaphase inhibitor, which are subsequently
            degraded by the 26S proteasome. Biochemical studies have shown that
            the vertebrate APC contains eight subunits. The composition of the
            APC is highly conserved in organisms from yeast to humans. The
            exact function of this gene product is not known. Two transcript
            variants encoding different isoforms have been found for this gene.
            [provided by RefSeq, Nov 2013].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice junction at the 3' end of an exon compared to variant 1. The
            resulting isoform (2) has the same N- and C-termini but is 1 aa
            shorter compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC059383.1, SRR1803612.59956.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000315368.8/ ENSP00000318775.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-360               BI546948.1         43-402
            361-2212            BC059383.1         371-2222
            2213-2635           CA312572.1         18-440              c
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4p15.2"
     gene            1..2635
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /note="anaphase promoting complex subunit 4"
                     /db_xref="GeneID:29945"
                     /db_xref="HGNC:HGNC:19990"
                     /db_xref="MIM:606947"
     exon            1..82
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            83..221
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     CDS             93..2519
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /note="isoform 2 is encoded by transcript variant 2;
                     cyclosome subunit 4"
                     /codon_start=1
                     /product="anaphase-promoting complex subunit 4 isoform 2"
                     /protein_id="NP_037499.2"
                     /db_xref="CCDS:CCDS3434.1"
                     /db_xref="GeneID:29945"
                     /db_xref="HGNC:HGNC:19990"
                     /db_xref="MIM:606947"
                     /translation="MLRFPTCFPSFRVVGEKQLPQEIIFLVWSPKRDLIALANTAGEV
                     LLHRLASFHRVWSFPPNENTGKEVTCLAWRPDGKLLAFALADTKKIVLCDVEKPESLH
                     SFSVEAPVSCMHWMEVTVESSVLTSFYNAEDESNLLLPKLPTLPKNYSNTSKIFSEEN
                     SDEIIKLLGDVRLNILVLGGSSGFIELYAYGMFKIARVTGIAGTCLALCLSSDLKSLS
                     VVTEVSTNGASEVSYFQLETNLLYSFLPEVTRMARKFTHISALLQYINLSLTCMCEAW
                     EEILMQMDSRLTKFVQEKNTTTSVQDEFMHLLLWGKASAELQTLLMNQLTVKGLKKLG
                     QSIESSYSSIQKLVISHLQSGSESLLYHLSELKGMASWKQKYEPLGLDAAGIEEAITA
                     VGSFILKANELLQVIDSSMKNFKAFFRWLYVAMLRMTEDHVLPELNKMTQKDITFVAE
                     FLTEHFNEAPDLYNRKGKYFNVERVGQYLKDEDDDLVSPPNTEGNQWYDFLQNSSHLK
                     ESPLLFPYYPRKSLHFVKRRMENIIDQCLQKPADVIGKSMNQAICIPLYRDTRSEDST
                     RRLFKFPFLWNNKTSNLHYLLFTILEDSLYKMCILRRHTDISQSVSNGLIAIKFGSFT
                     YATTEKVRRSIYSCLDAQFYDDETVTVVLKDTVGREGRDRLLVQLPLSLVYNSEDSAE
                     YQFTGTYSTRLDEQCSAIPTRTMHFEKHWRLLESMKAQYVAGNGFRKVSCVLSSNLRH
                     VRVFEMDIDDEWELDESSDEEEEASNKPVKIKEEVLSESEAENQQAGAAALAPEIVIK
                     VEKLDPELDS"
     exon            222..327
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            328..460
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            461..535
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            536..562
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            563..607
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            608..692
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            693..797
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            798..881
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            882..968
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            969..1033
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1034..1076
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1153
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1254
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1255..1306
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1307..1362
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1363..1409
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1410..1466
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1467..1523
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1524..1617
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1618..1715
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1716..1777
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1778..1816
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1817..1918
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1919..1993
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            1994..2167
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2168..2291
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     exon            2292..2635
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2610..2615
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /note="hexamer: AATAAA"
     polyA_site      2635
                     /gene="ANAPC4"
                     /gene_synonym="APC4"
                     /note="major polyA site"
ORIGIN      
        1 ctggaggctg tggcgcgcgg ccggcagagg gaggggagag gccactgggg ccgtgttagt
       61 ctgccggtgg ggactcttgc agggccgtcc ccatgttgcg ttttccgacc tgtttcccat
      121 ccttccgggt ggtgggagag aagcagctcc cgcaggagat tattttcctg gtctggtcgc
      181 ccaagcggga tctcattgct ttggccaaca cagctggcga ggttttactt catcgactgg
      241 caagttttca tcgagtttgg agttttccac caaatgaaaa tacaggaaag gaggtgacgt
      301 gtctggcatg gagaccagat ggcaaacttt tggcctttgc tcttgctgat accaagaaaa
      361 ttgttttgtg tgatgtagaa aaacctgaga gcttacactc tttttctgtg gaggctccag
      421 tttcctgtat gcattggatg gaagtgacag tagaaagcag tgttctcaca tcattttata
      481 atgctgagga tgaatcaaat cttctcttac ctaaactacc tacactgcca aaaaactata
      541 gcaacacctc aaaaatattt agtgaagaaa attctgatga aattattaag ctcttgggag
      601 acgtcaggct taatattctc gtccttggag gaagctctgg atttattgag ctttatgctt
      661 atggaatgtt taaaattgct cgagtcacag ggattgctgg tacttgtctt gcattatgtt
      721 tatcaagtga tttgaaatca ttatcagtgg tcacagaagt ctctaccaat ggtgcttcag
      781 aagtttcata ctttcagctt gaaactaatc tgttgtactc tttcttacct gaagtaactc
      841 ggatggccag aaagtttact catatttcag ctctgttaca gtatataaat ttgtcactaa
      901 catgtatgtg tgaagcatgg gaagaaatac taatgcagat ggattctcgt ctcaccaagt
      961 ttgtgcagga aaagaacaca accacatcag tgcaagatga gttcatgcac ttgctattat
     1021 gggggaaagc aagtgctgaa cttcagactc tcttgatgaa tcagttaaca gtaaagggct
     1081 tgaaaaagct tggccagtct atagagtcat catactccag tatacaaaaa ttggtcataa
     1141 gtcatttaca gagtggctcg gagtctttat tataccattt gagtgaattg aaaggaatgg
     1201 cttcatggaa gcaaaaatat gaacctcttg gactagatgc tgcaggaatc gaagaagcta
     1261 taactgctgt gggttctttt atactcaagg caaatgaact tcttcaagtt atagatagta
     1321 gtatgaaaaa cttcaaagca ttttttcggt ggctttatgt ggcaatgttg agaatgacag
     1381 aagaccatgt gcttcccgag ctgaataaga tgactcagaa agatatcaca tttgttgctg
     1441 aatttcttac tgaacatttc aatgaggctc cagaccttta taatcgaaaa ggaaaatact
     1501 ttaacgttga aagagttggt cagtacttga aagatgaaga tgatgatctt gtgtcacccc
     1561 ctaacacaga aggaaaccag tggtatgact ttcttcaaaa tagcagccac cttaaagaaa
     1621 gtcctttgct gtttccttat tatcctcgaa aatcattgca ttttgtgaaa aggcggatgg
     1681 agaatattat tgatcagtgt ttgcaaaagc cagcagatgt aattggaaaa tcgatgaatc
     1741 aagcaatctg tattccattg tatagagata ccagaagtga ggattctaca cgtagattgt
     1801 tcaaatttcc ttttctgtgg aataataaaa cttcaaatct acattatctt ctttttacta
     1861 ttctagaaga ttcactttat aaaatgtgca tcttaaggag acatactgat atttctcaat
     1921 ctgtgagtaa tggactaatt gctattaaat ttgggagctt tacatatgcc acaacagaaa
     1981 aagtcagaag aagcatctac agttgtttag atgcacagtt ttatgatgat gaaactgtaa
     2041 cagtagttct taaagacact gtaggacgtg aaggaagaga tagactcttg gtccagctgc
     2101 ctttgtcttt agtatataac agtgaagatt ctgcagaata tcagttcact gggacttatt
     2161 ctacaaggct agatgaacag tgtagtgcta ttcccacccg taccatgcat tttgagaagc
     2221 actggagatt actggaaagt atgaaagcac agtatgttgc tgggaatggt tttcgaaaag
     2281 tgtcctgtgt gttaagctca aatcttcgtc atgtgagagt atttgaaatg gacatagatg
     2341 atgaatggga gctcgatgag tcttcagatg aagaggagga ggccagtaat aagcctgtaa
     2401 aaataaagga agaagtgttg tcggagtcag aggcagagaa ccaacaagct ggtgctgccg
     2461 ctttagctcc agagatagtc attaaagtgg aaaaacttga ccctgagcta gactcctaat
     2521 ctagcttgcc attattgtgt gtgtaattat ggccaaaagg acataggaga tggactaaga
     2581 tgtcttggac cacctttgtg taacaaagaa ataaacagta aattttattt tttca
//
