LOCUS       NM_013366               2655 bp    mRNA    linear   PRI 03-MAY-2024
DEFINITION  Homo sapiens anaphase promoting complex subunit 2 (ANAPC2), mRNA.
ACCESSION   NM_013366 XM_942539 XM_946085 XM_946087 XM_946089
VERSION     NM_013366.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Wu,H., Zhang,X., Shen,Q., Liu,Y., Gao,Y., Wang,G., Lv,M., Hua,R.,
            Xu,Y., Zhou,P., Wei,Z., Tao,F., He,X., Cao,Y. and Liu,M.
  TITLE     A homozygous loss-of-function mutation in FBXO43 causes human
            non-obstructive azoospermia
  JOURNAL   Clin Genet 101 (1), 55-64 (2022)
   PUBMED   34595750
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 2655)
  AUTHORS   Yau,R.G., Doerner,K., Castellanos,E.R., Haakonsen,D.L., Werner,A.,
            Wang,N., Yang,X.W., Martinez-Martin,N., Matsumoto,M.L., Dixit,V.M.
            and Rape,M.
  TITLE     Assembly and Function of Heterotypic Ubiquitin Chains in Cell-Cycle
            and Protein Quality Control
  JOURNAL   Cell 171 (4), 918-933 (2017)
   PUBMED   29033132
REFERENCE   4  (bases 1 to 2655)
  AUTHORS   Zhang,S., Chang,L., Alfieri,C., Zhang,Z., Yang,J., Maslen,S.,
            Skehel,M. and Barford,D.
  TITLE     Molecular mechanism of APC/C activation by mitotic phosphorylation
  JOURNAL   Nature 533 (7602), 260-264 (2016)
   PUBMED   27120157
REFERENCE   5  (bases 1 to 2655)
  AUTHORS   Chang,L., Zhang,Z., Yang,J., McLaughlin,S.H. and Barford,D.
  TITLE     Atomic structure of the APC/C and its mechanism of protein
            ubiquitination
  JOURNAL   Nature 522 (7557), 450-454 (2015)
   PUBMED   26083744
REFERENCE   6  (bases 1 to 2655)
  AUTHORS   Kramer,E.R., Scheuringer,N., Podtelejnikov,A.V., Mann,M. and
            Peters,J.M.
  TITLE     Mitotic regulation of the APC activator proteins CDC20 and CDH1
  JOURNAL   Mol Biol Cell 11 (5), 1555-1569 (2000)
   PUBMED   10793135
REFERENCE   7  (bases 1 to 2655)
  AUTHORS   Lukas,C., Sorensen,C.S., Kramer,E., Santoni-Rugiu,E., Lindeneg,C.,
            Peters,J.M., Bartek,J. and Lukas,J.
  TITLE     Accumulation of cyclin B1 requires E2F and cyclin-A-dependent
            rearrangement of the anaphase-promoting complex
  JOURNAL   Nature 401 (6755), 815-818 (1999)
   PUBMED   10548110
REFERENCE   8  (bases 1 to 2655)
  AUTHORS   Gieffers,C., Peters,B.H., Kramer,E.R., Dotti,C.G. and Peters,J.M.
  TITLE     Expression of the CDH1-associated form of the anaphase-promoting
            complex in postmitotic neurons
  JOURNAL   Proc Natl Acad Sci U S A 96 (20), 11317-11322 (1999)
   PUBMED   10500174
REFERENCE   9  (bases 1 to 2655)
  AUTHORS   Grossberger,R., Gieffers,C., Zachariae,W., Podtelejnikov,A.V.,
            Schleiffer,A., Nasmyth,K., Mann,M. and Peters,J.M.
  TITLE     Characterization of the DOC1/APC10 subunit of the yeast and the
            human anaphase-promoting complex
  JOURNAL   J Biol Chem 274 (20), 14500-14507 (1999)
   PUBMED   10318877
REFERENCE   10 (bases 1 to 2655)
  AUTHORS   Yu,H., Peters,J.M., King,R.W., Page,A.M., Hieter,P. and
            Kirschner,M.W.
  TITLE     Identification of a cullin homology region in a subunit of the
            anaphase-promoting complex
  JOURNAL   Science 279 (5354), 1219-1222 (1998)
   PUBMED   9469815
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BM925063.1 and BC032503.1.
            
            On Nov 22, 2018 this sequence version replaced NM_013366.3.
            
            Summary: A large protein complex, termed the anaphase-promoting
            complex (APC), or the cyclosome, promotes metaphase-anaphase
            transition by ubiquitinating its specific substrates such as
            mitotic cyclins and anaphase inhibitor, which are subsequently
            degraded by the 26S proteasome. Biochemical studies have shown that
            the vertebrate APC contains eight subunits. The composition of the
            APC is highly conserved in organisms from yeast to humans. The
            product of this gene is a component of the complex and shares
            sequence similarity with a recently identified family of proteins
            called cullins, which may also be involved in ubiquitin-mediated
            degradation. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC032503.1, AF191337.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000323927.3/ ENSP00000314004.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-145               BM925063.1         45-189
            146-2655            BC032503.1         123-2632
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.3"
     gene            1..2655
                     /gene="ANAPC2"
                     /gene_synonym="APC2"
                     /note="anaphase promoting complex subunit 2"
                     /db_xref="GeneID:29882"
                     /db_xref="HGNC:HGNC:19989"
                     /db_xref="MIM:606946"
     exon            1..145
                     /gene="ANAPC2"
                     /gene_synonym="APC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             29..2497
                     /gene="ANAPC2"
                     /gene_synonym="APC2"
                     /note="cyclosome subunit 2"
                     /codon_start=1
                     /product="anaphase-promoting complex subunit 2"
                     /protein_id="NP_037498.1"
                     /db_xref="CCDS:CCDS7033.1"
                     /db_xref="GeneID:29882"
                     /db_xref="HGNC:HGNC:19989"
                     /db_xref="MIM:606946"
                     /translation="MAAAVVVAEGDSDSRPGQELLVAWNTVSTGLVPPAALGLVSSRT
                     SGAVPPKEEELRAAVEVLRGHGLHSVLEEWFVEVLQNDLQANISPEFWNAISQCENSA
                     DEPQCLLLLLDAFGLLESRLDPYLRSLELLEKWTRLGLLMGTGAQGLREEVHTMLRGV
                     LFFSTPRTFQEMIQRLYGCFLRVYMQSKRKGEGGTDPELEGELDSRYARRRYYRLLQS
                     PLCAGCSSDKQQCWCRQALEQFHQLSQVLHRLSLLERVSAEAVTTTLHQVTRERMEDR
                     CRGEYERSFLREFHKWIERVVGWLGKVFLQDGPARPASPEAGNTLRRWRCHVQRFFYR
                     IYASLRIEELFSIVRDFPDSRPAIEDLKYCLERTDQRQQLLVSLKAALETRLLHPGVN
                     TCDIITLYISAIKALRVLDPSMVILEVACEPIRRYLRTREDTVRQIVAGLTGDSDGTG
                     DLAVELSKTDPASLETGQDSEDDSGEPEDWVPDPVDADPGKSSSKRRSSDIISLLVSI
                     YGSKDLFINEYRSLLADRLLHQFSFSPEREIRNVELLKLRFGEAPMHFCEVMLKDMAD
                     SRRINANIREEDEKRPAEEQPPFGVYAVILSSEFWPPFKDEKLEVPEDIRAALEAYCK
                     KYEQLKAMRTLSWKHTLGLVTMDVELADRTLSVAVTPVQAVILLYFQDQASWTLEELS
                     KAVKMPVALLRRRMSVWLQQGVLREEPPGTFSVIEEERPQDRDNMVLIDSDDESDSGM
                     ASQADQKEEELLLFWTYIQAMLTNLESLSLDRIYNMLRMFVVTGPALAEIDLQELQGY
                     LQKKVRDQQLVYSAGVYRLPKNCS"
     exon            146..768
                     /gene="ANAPC2"
                     /gene_synonym="APC2"
                     /inference="alignment:Splign:2.1.0"
     exon            769..901
                     /gene="ANAPC2"
                     /gene_synonym="APC2"
                     /inference="alignment:Splign:2.1.0"
     exon            902..1076
                     /gene="ANAPC2"
                     /gene_synonym="APC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1196
                     /gene="ANAPC2"
                     /gene_synonym="APC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..1314
                     /gene="ANAPC2"
                     /gene_synonym="APC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1315..1496
                     /gene="ANAPC2"
                     /gene_synonym="APC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1497..1638
                     /gene="ANAPC2"
                     /gene_synonym="APC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1639..1714
                     /gene="ANAPC2"
                     /gene_synonym="APC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1715..1918
                     /gene="ANAPC2"
                     /gene_synonym="APC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1919..2048
                     /gene="ANAPC2"
                     /gene_synonym="APC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2049..2284
                     /gene="ANAPC2"
                     /gene_synonym="APC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2285..2655
                     /gene="ANAPC2"
                     /gene_synonym="APC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2635..2640
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANAPC2"
                     /gene_synonym="APC2"
                     /note="hexamer: ATTAAA"
     polyA_site      2655
                     /gene="ANAPC2"
                     /gene_synonym="APC2"
                     /note="major polyA site"
ORIGIN      
        1 gagcgaatct cggagtcggt gggtgcagat ggcggcggca gttgtggtgg cggaggggga
       61 cagcgactcc cggcccggac aggagttgtt agtggcctgg aacaccgtga gcaccggcct
      121 ggtgccgccg gctgcgctgg ggctggtgtc ttcccggacc agcggtgcag tcccgccaaa
      181 ggaagaggag ctccgggcgg cggtggaggt tctgaggggc cacgggctac actcggtcct
      241 ggaggagtgg ttcgtggagg tgctgcagaa cgatctgcag gccaacatct cccctgagtt
      301 ctggaatgcc atctcccaat gcgagaactc tgcggatgag ccccagtgcc ttttgctact
      361 ccttgacgct tttggcctgc tggagagccg cctggatccc tacctgcgta gcctagagct
      421 gctggagaaa tggactcgcc tgggcttgct gatgggcact ggtgctcagg ggctgcgaga
      481 agaagtccac actatgttgc gcggagtctt gttctttagc acccccagaa ccttccaaga
      541 gatgatccag cgtctgtatg ggtgcttctt gagagtctat atgcagagta agaggaaggg
      601 ggaagggggc acagacccgg aactggaagg ggagctggac agccggtatg cccgtcgccg
      661 gtactaccgg ctcctgcaga gcccgctgtg tgcagggtgc agcagtgaca agcaacagtg
      721 ctggtgtcgc caggctctgg agcagttcca tcagctcagc caggtcttac acaggctcag
      781 tctgctggag cgggtcagtg ccgaggctgt gaccaccacc ctgcaccagg tgacccggga
      841 gaggatggag gaccgttgcc ggggcgagta cgagcgctcc ttcctgcgtg agttccacaa
      901 gtggatcgag cgggtggtcg gctggctcgg caaggtgttc ctgcaggacg gccccgccag
      961 gcccgcatct cccgaggccg gcaacaccct gcgccgctgg cgctgccacg tgcaaaggtt
     1021 cttctaccgc atctacgcca gcctgcgcat cgaggagctc ttcagcatcg tccgagactt
     1081 cccagactcc cggccagcca tcgaggacct caagtactgc ctggagagga cggaccagag
     1141 gcagcagctg ctcgtgtccc tcaaggctgc cctggagact cggctcctgc atccaggcgt
     1201 caacacgtgt gacatcatca ccctctatat ctctgccatc aaggcgctgc gcgtgctgga
     1261 cccttccatg gtcatcctgg aggtggcctg tgagcctatc cgccgctacc tgaggacgcg
     1321 ggaggacaca gtgcggcaga ttgtggctgg gctgacgggg gactcggacg ggacagggga
     1381 cctggctgtt gagctgtcca agaccgaccc ggcgagcctg gagacaggcc aggacagtga
     1441 ggatgactca ggcgagccag aggactgggt cccggaccct gtggatgccg atccagggaa
     1501 gtcgagctcc aagcggcgtt catcggacat catcagcctg ctggtcagca tctacggcag
     1561 caaggacctc ttcatcaatg agtaccgctc gctgctggcc gaccgcctgc tgcaccagtt
     1621 cagcttcagc cccgagcggg agatccgcaa cgtggagctg ctgaagctgc gctttggcga
     1681 ggccccaatg cacttctgtg aagtcatgct gaaggacatg gcggactccc gccgcatcaa
     1741 tgccaacatc cgggaggagg atgagaagcg gccagcagag gagcagccac cgttcggggt
     1801 ctacgctgtc atcctgtcca gtgagttctg gccgcccttc aaggacgaga agctggaggt
     1861 ccccgaggat atcagggcag ccctggaggc ttactgcaag aagtatgagc agctcaaggc
     1921 catgcggacc ctcagttgga agcacaccct gggcctggtg accatggacg tggagctggc
     1981 cgaccgcacg ctgtctgtgg cggtcacccc agtacaggcg gtgatcttgc tgtattttca
     2041 ggaccaagcc agctggaccc tggaggaact gagcaaggcg gtgaagatgc ccgtggcgct
     2101 gctgcggcgg cggatgtccg tgtggctgca gcagggtgtg ctgcgtgagg agccccccgg
     2161 caccttctct gtcattgagg aggagcggcc tcaggaccgg gacaacatgg tgctcattga
     2221 cagtgacgac gagagcgact ccggcatggc ctcccaggcc gaccagaagg aggaggagct
     2281 gctgctcttc tggacgtaca tccaggccat gctgaccaac ctggagagcc tctcactgga
     2341 tcgtatctac aacatgctcc gcatgtttgt ggtgactggg cctgcactgg ccgagattga
     2401 cctgcaggag ctgcagggct acctgcagaa gaaggtgcgg gaccagcagc tcgtctactc
     2461 ggccggcgtc taccgcctgc ccaagaactg cagctgacac atcgcccgcc cgcccgcccg
     2521 cccgccaggc gctgccctgc aggtgctctc gtcctcccgt gccagccccc gcccgcccgt
     2581 gtcccagaat gcactgctga ggagcatgcc cacccccacc cccgcagtgt gcagattaaa
     2641 gcaagtcaga tcatc
//
