LOCUS       NM_173473               3231 bp    mRNA    linear   PRI 03-MAY-2024
DEFINITION  Homo sapiens anaphase promoting complex subunit 16 (ANAPC16),
            transcript variant 2, mRNA.
ACCESSION   NM_173473
VERSION     NM_173473.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Wild,T., Budzowska,M., Hellmuth,S., Eibes,S., Karemore,G.,
            Barisic,M., Stemmann,O. and Choudhary,C.
  TITLE     Deletion of APC7 or APC16 Allows Proliferation of Human Cells
            without the Spindle Assembly Checkpoint
  JOURNAL   Cell Rep 25 (9), 2317-2328 (2018)
   PUBMED   30485802
  REMARK    GeneRIF: the contribution of the anaphase-promoting
            complex/cyclosome subunits APC7 and APC16 to APC/C composition and
            function in human cells.
REFERENCE   3  (bases 1 to 3231)
  AUTHORS   Yau,R.G., Doerner,K., Castellanos,E.R., Haakonsen,D.L., Werner,A.,
            Wang,N., Yang,X.W., Martinez-Martin,N., Matsumoto,M.L., Dixit,V.M.
            and Rape,M.
  TITLE     Assembly and Function of Heterotypic Ubiquitin Chains in Cell-Cycle
            and Protein Quality Control
  JOURNAL   Cell 171 (4), 918-933 (2017)
   PUBMED   29033132
REFERENCE   4  (bases 1 to 3231)
  AUTHORS   Zhang,S., Chang,L., Alfieri,C., Zhang,Z., Yang,J., Maslen,S.,
            Skehel,M. and Barford,D.
  TITLE     Molecular mechanism of APC/C activation by mitotic phosphorylation
  JOURNAL   Nature 533 (7602), 260-264 (2016)
   PUBMED   27120157
REFERENCE   5  (bases 1 to 3231)
  AUTHORS   Yamaguchi,M., Yu,S., Qiao,R., Weissmann,F., Miller,D.J.,
            VanderLinden,R., Brown,N.G., Frye,J.J., Peters,J.M. and
            Schulman,B.A.
  TITLE     Structure of an APC3-APC16 complex: insights into assembly of the
            anaphase-promoting complex/cyclosome
  JOURNAL   J Mol Biol 427 (8), 1748-1764 (2015)
   PUBMED   25490258
  REMARK    GeneRIF: The structures show how one APC16 binds asymmetrically to
            the symmetric APC3 dimer and, together with biochemistry and prior
            data, explain how APC16 recruits APC7 to APC3.
REFERENCE   6  (bases 1 to 3231)
  AUTHORS   Cui,X.Y., Chen,J.X., Liu,B.W., Xiao,L.Y. and Fang,D.Z.
  TITLE     Identification of a novel gene, MSAG, regulated by high levels of
            glucose and insulin
  JOURNAL   Biochemistry (Mosc) 74 (1), 22-28 (2009)
   PUBMED   19232044
  REMARK    GeneRIF: The predicted protein encoded by MSAG contains 110 amino
            acids and has a theoretical molecular weight of 11667.04 and an
            isoelectric point of 4.91 (MSAG).
REFERENCE   7  (bases 1 to 3231)
  AUTHORS   Oh,J.H., Yang,J.O., Hahn,Y., Kim,M.R., Byun,S.S., Jeon,Y.J.,
            Kim,J.M., Song,K.S., Noh,S.M., Kim,S., Yoo,H.S., Kim,Y.S. and
            Kim,N.S.
  TITLE     Transcriptome analysis of human gastric cancer
  JOURNAL   Mamm Genome 16 (12), 942-954 (2005)
   PUBMED   16341674
REFERENCE   8  (bases 1 to 3231)
  AUTHORS   Ozyildirim,A.M., Wistow,G.J., Gao,J., Wang,J., Dickinson,D.P.,
            Frierson,H.F. Jr. and Laurie,G.W.
  TITLE     The lacrimal gland transcriptome is an unusually rich source of
            rare and poorly characterized gene transcripts
  JOURNAL   Invest Ophthalmol Vis Sci 46 (5), 1572-1580 (2005)
   PUBMED   15851553
REFERENCE   9  (bases 1 to 3231)
  AUTHORS   Castro,A., Bernis,C., Vigneron,S., Labbe,J.C. and Lorca,T.
  TITLE     The anaphase-promoting complex: a key factor in the regulation of
            cell cycle
  JOURNAL   Oncogene 24 (3), 314-325 (2005)
   PUBMED   15678131
  REMARK    Review article
REFERENCE   10 (bases 1 to 3231)
  AUTHORS   Deloukas,P., Earthrowl,M.E., Grafham,D.V., Rubenfield,M.,
            French,L., Steward,C.A., Sims,S.K., Jones,M.C., Searle,S.,
            Scott,C., Howe,K., Hunt,S.E., Andrews,T.D., Gilbert,J.G.,
            Swarbreck,D., Ashurst,J.L., Taylor,A., Battles,J., Bird,C.P.,
            Ainscough,R., Almeida,J.P., Ashwell,R.I., Ambrose,K.D.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Bates,K.,
            Beasley,H., Bray-Allen,S., Brown,A.J., Brown,J.Y., Burford,D.C.,
            Burrill,W., Burton,J., Cahill,P., Camire,D., Carter,N.P.,
            Chapman,J.C., Clark,S.Y., Clarke,G., Clee,C.M., Clegg,S., Corby,N.,
            Coulson,A., Dhami,P., Dutta,I., Dunn,M., Faulkner,L., Frankish,A.,
            Frankland,J.A., Garner,P., Garnett,J., Gribble,S., Griffiths,C.,
            Grocock,R., Gustafson,E., Hammond,S., Harley,J.L., Hart,E.,
            Heath,P.D., Ho,T.P., Hopkins,B., Horne,J., Howden,P.J., Huckle,E.,
            Hynds,C., Johnson,C., Johnson,D., Kana,A., Kay,M., Kimberley,A.M.,
            Kershaw,J.K., Kokkinaki,M., Laird,G.K., Lawlor,S., Lee,H.M.,
            Leongamornlert,D.A., Laird,G., Lloyd,C., Lloyd,D.M., Loveland,J.,
            Lovell,J., McLaren,S., McLay,K.E., McMurray,A.,
            Mashreghi-Mohammadi,M., Matthews,L., Milne,S., Nickerson,T.,
            Nguyen,M., Overton-Larty,E., Palmer,S.A., Pearce,A.V., Peck,A.I.,
            Pelan,S., Phillimore,B., Porter,K., Rice,C.M., Rogosin,A.,
            Ross,M.T., Sarafidou,T., Sehra,H.K., Shownkeen,R., Skuce,C.D.,
            Smith,M., Standring,L., Sycamore,N., Tester,J., Thorpe,A.,
            Torcasso,W., Tracey,A., Tromans,A., Tsolas,J., Wall,M., Walsh,J.,
            Wang,H., Weinstock,K., West,A.P., Willey,D.L., Whitehead,S.L.,
            Wilming,L., Wray,P.W., Young,L., Chen,Y., Lovering,R.C.,
            Moschonas,N.K., Siebert,R., Fechtel,K., Bentley,D., Durbin,R.,
            Hubbard,T., Doucette-Stamm,L., Beck,S., Smith,D.R. and Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 10
  JOURNAL   Nature 429 (6990), 375-381 (2004)
   PUBMED   15164054
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA739927.1, AL607035.12 and CD724246.1.
            
            On Nov 23, 2018 this sequence version replaced NM_173473.3.
            
            Transcript Variant: This variant (2) differs in the 5' UTR,
            compared to variant 1, and encodes isoform 1. Variants 1-3 encode
            the same protein (isoform 1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.211521.1,
                                           SRR1660803.43517.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000299381.5/ ENSP00000299381.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                DA739927.1         13-42
            31-127              AL607035.12        39793-39889
            128-296             AL607035.12        47639-47807
            297-371             AL607035.12        54117-54191
            372-2952            AL607035.12        56752-59332
            2953-3231           CD724246.1         1-279
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q22.1"
     gene            1..3231
                     /gene="ANAPC16"
                     /gene_synonym="APC16; bA570G20.3; C10orf104; CENP-27;
                     MSAG"
                     /note="anaphase promoting complex subunit 16"
                     /db_xref="GeneID:119504"
                     /db_xref="HGNC:HGNC:26976"
                     /db_xref="MIM:613427"
     exon            1..127
                     /gene="ANAPC16"
                     /gene_synonym="APC16; bA570G20.3; C10orf104; CENP-27;
                     MSAG"
                     /inference="alignment:Splign:2.1.0"
     exon            128..296
                     /gene="ANAPC16"
                     /gene_synonym="APC16; bA570G20.3; C10orf104; CENP-27;
                     MSAG"
                     /inference="alignment:Splign:2.1.0"
     CDS             155..487
                     /gene="ANAPC16"
                     /gene_synonym="APC16; bA570G20.3; C10orf104; CENP-27;
                     MSAG"
                     /note="isoform 1 is encoded by transcript variant 2;
                     metabolic syndrome-associated protein; cyclosome subunit
                     16; centromere protein 27"
                     /codon_start=1
                     /product="anaphase-promoting complex subunit 16 isoform 1"
                     /protein_id="NP_775744.1"
                     /db_xref="CCDS:CCDS7314.1"
                     /db_xref="GeneID:119504"
                     /db_xref="HGNC:HGNC:26976"
                     /db_xref="MIM:613427"
                     /translation="MAASSSSSSAGGVSGSSVTGSGFSVSDLAPPRKALFTYPKGAGE
                     MLEDGSERFLCESVFSYQVASTLKQVKHDQQVARMEKLAGLVEELEADEWRFKPIEQL
                     LGFTPSSG"
     exon            297..371
                     /gene="ANAPC16"
                     /gene_synonym="APC16; bA570G20.3; C10orf104; CENP-27;
                     MSAG"
                     /inference="alignment:Splign:2.1.0"
     exon            372..3231
                     /gene="ANAPC16"
                     /gene_synonym="APC16; bA570G20.3; C10orf104; CENP-27;
                     MSAG"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1175..1180
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANAPC16"
                     /gene_synonym="APC16; bA570G20.3; C10orf104; CENP-27;
                     MSAG"
                     /note="hexamer: AATAAA"
     polyA_site      1200
                     /gene="ANAPC16"
                     /gene_synonym="APC16; bA570G20.3; C10orf104; CENP-27;
                     MSAG"
                     /note="major polyA site"
     regulatory      3213..3218
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANAPC16"
                     /gene_synonym="APC16; bA570G20.3; C10orf104; CENP-27;
                     MSAG"
                     /note="hexamer: AACAAA"
     polyA_site      3231
                     /gene="ANAPC16"
                     /gene_synonym="APC16; bA570G20.3; C10orf104; CENP-27;
                     MSAG"
ORIGIN      
        1 acgccgcctt cgccgctggc tccgtctgtt ggggggcgaa cacgccgcgg tcctcgtcgt
       61 ggtgagcgca gccactcagg ctggtcctgg gggtggggct gtaggggaaa gtgctaaagc
      121 cgctgagtga agtaagaact ctgctagaga ggaaatggct gcttcatcat catcctcctc
      181 agctggtggg gtcagtggaa gttctgtcac tggatctggt ttcagtgtct cagaccttgc
      241 cccaccacgg aaagcccttt tcacctaccc caaaggagct ggagagatgt tagaagatgg
      301 ctctgagaga ttcctctgcg aatctgtttt tagctatcaa gtggcatcca cgcttaaaca
      361 ggtgaaacat gatcagcaag ttgctcggat ggaaaaacta gctggtttgg tagaagagct
      421 ggaggctgac gagtggcggt ttaagcccat cgagcagctg ctgggattca ccccctcttc
      481 aggttgatac tgcctggatg gtcacctctg gtgcgcagca agtgcaaagc cagtggggga
      541 ctttctcaca gcttacatag ccatccagag atccacagct acgtcactga attgttaatg
      601 cacatttgta cttggtttct ctgtatctat tcacaggcaa caaatactta tatgtgtgat
      661 ctttcaggga atgttttgtt tatttgtttt taaaagtatt gggaatcaga ttaagacaat
      721 cagtttcaga gaaccaggag gtttggggtt aagagatact caaaaatttt cacaagccaa
      781 gtagggcata tatcagattt ggccaactga atggcgtctg tcctgtcatc catatggtgc
      841 ctggaaatat ttaccagtca aggtcaaggt cagcatctgt ggttaaaaat atagcattct
      901 gacctaaaaa agttattttg cagatgaatg tgttttcaac tcaggaccta tccaaatgag
      961 gaatttttaa atattctttt ttttttccta tttttagaca tcaattctat agattctgac
     1021 tttttctaac ctcttataga catgccaaat gctggcaaaa agaagtgctt tttggatatg
     1081 gcagcacttg taaaaataaa gcagtaagca aaatcctttt aaacacagaa atcctgagtt
     1141 cttctcattg gtggactcaa gcaattctgt agcaaataaa tcctttgaaa gagctccaaa
     1201 ttggtggcat tatcctttca aaatctcagg gatttgggat gaagtgaaga ggtcaaatta
     1261 ctttttagaa gaagggaatc taaaaaccat ctctcctaaa caaatggtag actggcttta
     1321 cttaagggat atttgtcttt ataggagtac ataaatttat cttaatgata tttaagtagt
     1381 tttttttttt ttttgagacg aagttttgct cttgttgccc aggctgtatc gcaatggcgc
     1441 gatctctgct cactgcaaca tccacctccc aggttcaagc aattctcctg cctcagcctc
     1501 ccaagtagct gggattacag gcacccgcca ccacacccag ctaatttttt gtatatttag
     1561 tagagacggg gtttcaccat gttgaccagg ctagtctcga acttctgacc tcaggtgatc
     1621 cacctgcctc agcctcccaa attgctggga ttacaggcgt gagccacctt gcccagccaa
     1681 ttttttttta aagagacatg gtcttgctat gttgcccagg ttggtcttga actcctggcc
     1741 tcaagagatc cccccaacct tggcctccaa agtgctggga ttacaggcat gagccaccgc
     1801 gcctggcctc aagtaagttt ttataccatg ataggggaaa acctggaagg ctaagagatg
     1861 tgctgttttc cttcatggga ccccactttc cacagtaaac aactggtata atcatttatt
     1921 acgttgtatg caaattttgc cttagacaac atttgtctgg ttccttatta ataacttgca
     1981 tctgatctgc ttagagttgc ttaattataa gtaaatataa tcccagtagc atttagcctt
     2041 aatctcaaga gttcaaaatg tttaacacat tctacctgac gggaggtggt gacctcattc
     2101 agtcggttaa gagcctgagc cctgtgaagt taaacacctg ctcatggtca catagcccag
     2161 gcagaagaaa atctcggact tggaatagat cccagttgcc tgataattca tctcattatt
     2221 gcctctaaag caaattggag actgggcaca gtggctccca cctttaatcc cagcacctgg
     2281 ggaggctgag gtgagtggat cactagaggt caggagttca agaccagtct ggccgacatg
     2341 gtgaaactca gtctctattg aaaatacaaa aattagctca gtgtggtggc acacacttgt
     2401 aatcccagct actcaggagg ctgaggcatg agaatcgctt aaacccagga ggcggaggtt
     2461 gcagtgagct gagatcacac cattgcactc tagtctgggt gacagagcga gactctgtct
     2521 caaaaaaaaa aaaataaagc cgggcgtggt ggctcacgcc tgtaatcccg gcactttggg
     2581 aggctgaggc aggcggattg cctgagatcg ggagttcgag accagcctgg ccaacatggt
     2641 gaaaccttgt ctctactaaa actacaaaaa aaattagcca ggcgtggtgg cgggcgcctg
     2701 taatcccagc tactcaggag gctgaggcag cagaatcgtt tgaacccggg aggcggaggt
     2761 tgcagtgagt ggagatcgtg cctgtgtgct ccagcctgag caacaagagc gaaactgtct
     2821 caaaaaaaag aagaaaataa ataaataaaa atcagagcac aagaagtacc agtccaccaa
     2881 ctcacatttt ctatattaat aattcttaac taaaagaagt tccatattcg acagcatttt
     2941 agttttttaa tagtacttat tagcatcaac catcttgata atcaccaagg gaaaaattaa
     3001 aaatacctag actgcaataa acctttaaat tggattttca gtgagaattt agaagcaatc
     3061 actacctttc tcgtttcatt tgtgtaacca tgcagcatag gcactgtgtt acatcatctt
     3121 tacgctttgg tatttgaact tattacagaa ttttttctct aaattggatg tttctttata
     3181 tgaccttgga aactgaagtg atcttagcat taaacaaaaa tgctttttga a
//
