LOCUS       NM_014042                801 bp    mRNA    linear   PRI 03-MAY-2024
DEFINITION  Homo sapiens anaphase promoting complex subunit 15 (ANAPC15),
            transcript variant 3, mRNA.
ACCESSION   NM_014042
VERSION     NM_014042.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 801)
  AUTHORS   Yau,R.G., Doerner,K., Castellanos,E.R., Haakonsen,D.L., Werner,A.,
            Wang,N., Yang,X.W., Martinez-Martin,N., Matsumoto,M.L., Dixit,V.M.
            and Rape,M.
  TITLE     Assembly and Function of Heterotypic Ubiquitin Chains in Cell-Cycle
            and Protein Quality Control
  JOURNAL   Cell 171 (4), 918-933 (2017)
   PUBMED   29033132
REFERENCE   3  (bases 1 to 801)
  AUTHORS   Li,Q., Chang,L., Aibara,S., Yang,J., Zhang,Z. and Barford,D.
  TITLE     WD40 domain of Apc1 is critical for the coactivator-induced
            allosteric transition that stimulates APC/C catalytic activity
  JOURNAL   Proc Natl Acad Sci U S A 113 (38), 10547-10552 (2016)
   PUBMED   27601667
  REMARK    GeneRIF: cryo-EM structure of an APC/C-Cdh1 complex with Apc1(WD40)
            deleted showed that the mutant APC/C is locked into an inactive
            conformation in which the UbcH10-binding site of the catalytic
            module is inaccessible. Additionally, an EM density for Apc15 is
            not visible
REFERENCE   4  (bases 1 to 801)
  AUTHORS   Zhang,S., Chang,L., Alfieri,C., Zhang,Z., Yang,J., Maslen,S.,
            Skehel,M. and Barford,D.
  TITLE     Molecular mechanism of APC/C activation by mitotic phosphorylation
  JOURNAL   Nature 533 (7602), 260-264 (2016)
   PUBMED   27120157
REFERENCE   5  (bases 1 to 801)
  AUTHORS   Uzunova,K., Dye,B.T., Schutz,H., Ladurner,R., Petzold,G.,
            Toyoda,Y., Jarvis,M.A., Brown,N.G., Poser,I., Novatchkova,M.,
            Mechtler,K., Hyman,A.A., Stark,H., Schulman,B.A. and Peters,J.M.
  TITLE     APC15 mediates CDC20 autoubiquitylation by APC/C(MCC) and
            disassembly of the mitotic checkpoint complex
  JOURNAL   Nat Struct Mol Biol 19 (11), 1116-1123 (2012)
   PUBMED   23007861
  REMARK    GeneRIF: APC15 is required for anaphase-promoting
            complex/cyclosome-bound mitotic checkpoint complex-dependent CDC20
            autoubiquitylation and degradation and for timely anaphase
            initiation.
REFERENCE   6  (bases 1 to 801)
  AUTHORS   Mansfeld,J., Collin,P., Collins,M.O., Choudhary,J.S. and Pines,J.
  TITLE     APC15 drives the turnover of MCC-CDC20 to make the spindle assembly
            checkpoint responsive to kinetochore attachment
  JOURNAL   Nat Cell Biol 13 (10), 1234-1243 (2011)
   PUBMED   21926987
  REMARK    GeneRIF: Anaphase promoting complex subunit 15(APC15) mediates the
            constant turnover of CDC20 and mitotic checkpoint protein
            complexes, allowing the spindle checkpoint assembly to respond to
            the attachment state of kinetochores.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 801)
  AUTHORS   Castro,A., Bernis,C., Vigneron,S., Labbe,J.C. and Lorca,T.
  TITLE     The anaphase-promoting complex: a key factor in the regulation of
            cell cycle
  JOURNAL   Oncogene 24 (3), 314-325 (2005)
   PUBMED   15678131
  REMARK    Review article
REFERENCE   8  (bases 1 to 801)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF077206.1.
            
            On Jun 2, 2019 this sequence version replaced NM_014042.2.
            
            Transcript Variant: This variant (3) uses an alternate in-frame
            splice junction at the 5' end of the last exon compared to variant
            1. The resulting isoform (b) has the same N- and C-termini but is
            shorter compared to isoform a. Variants 3-11 and 32-44 all encode
            the same isoform (b).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC005156.2, AF077206.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000227618.9/ ENSP00000227618.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-801               AF077206.1         83-883
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.4"
     gene            1..801
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /note="anaphase promoting complex subunit 15"
                     /db_xref="GeneID:25906"
                     /db_xref="HGNC:HGNC:24531"
                     /db_xref="MIM:614717"
     exon            1..46
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /inference="alignment:Splign:2.1.0"
     exon            47..131
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /inference="alignment:Splign:2.1.0"
     exon            132..261
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /inference="alignment:Splign:2.1.0"
     CDS             142..507
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /note="isoform b is encoded by transcript variant 3"
                     /codon_start=1
                     /product="anaphase-promoting complex subunit 15 isoform b"
                     /protein_id="NP_054761.1"
                     /db_xref="CCDS:CCDS8210.1"
                     /db_xref="GeneID:25906"
                     /db_xref="HGNC:HGNC:24531"
                     /db_xref="MIM:614717"
                     /translation="MSTLFPSLFPRVTETLWFNLDRPCVEETELQQQEQQHQAWLQSI
                     AEKDNNLVPIGKPASEHYDDEEEEDDEDDEDSEEDSEDDEDMQDMDEMNDYNESPDDG
                     EVNEVDMEGNEQDQDQWMI"
     exon            262..321
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /inference="alignment:Splign:2.1.0"
     exon            322..459
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /inference="alignment:Splign:2.1.0"
     exon            460..801
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /inference="alignment:Splign:2.1.0"
     regulatory      776..781
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /note="hexamer: AATAAA"
     polyA_site      801
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /note="major polyA site"
ORIGIN      
        1 gggtgaagcg cttccgggtc gccgccggca gcagcctccc ggcgcgatga agacactgag
       61 gctcagagag gttaagtgac tcagccaagg tcaaacagct agtaagtggt ggagccagga
      121 ctcaaagcca gtctaggagc catgtccact ttgttcccct cactcttccc tcgtgtgact
      181 gagactctgt ggtttaatct ggatcgaccc tgtgtggaag agacagagct gcagcagcag
      241 gaacagcagc atcaggcctg gctccaaagc atcgcggaga aagacaacaa cctggttcct
      301 attggcaagc cagcctcaga gcactatgat gacgaggaag aagaggatga tgaagatgat
      361 gaggatagtg aagaggactc agaggatgat gaggatatgc aggacatgga cgagatgaat
      421 gactacaatg agtcaccgga tgatggagag gtcaatgagg tggacatgga aggcaacgaa
      481 caggatcagg accagtggat gatctaggta gacaaggcag ggtggcctca gggagattcc
      541 aggccagccc aaactaccct gcatcccaac ccccaacccc tgcccacaga accagctgat
      601 ggccccagtg cctgaaagtg cccttgggca cctcctcagc tgctgccagg atctggtctc
      661 tttggcccct cccaggccat cagtctgcac ttgaaatccc cagggcctga aacctactcc
      721 accttcctgg ccagtacctc accccttgat tgccaggtct ggtctaagtt tctttaataa
      781 agacaaagga gtgattttcc a
//
