LOCUS       NM_001393459            1478 bp    mRNA    linear   PRI 03-MAY-2024
DEFINITION  Homo sapiens anaphase promoting complex subunit 15 (ANAPC15),
            transcript variant 45, mRNA.
ACCESSION   NM_001393459
VERSION     NM_001393459.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Yau,R.G., Doerner,K., Castellanos,E.R., Haakonsen,D.L., Werner,A.,
            Wang,N., Yang,X.W., Martinez-Martin,N., Matsumoto,M.L., Dixit,V.M.
            and Rape,M.
  TITLE     Assembly and Function of Heterotypic Ubiquitin Chains in Cell-Cycle
            and Protein Quality Control
  JOURNAL   Cell 171 (4), 918-933 (2017)
   PUBMED   29033132
REFERENCE   3  (bases 1 to 1478)
  AUTHORS   Li,Q., Chang,L., Aibara,S., Yang,J., Zhang,Z. and Barford,D.
  TITLE     WD40 domain of Apc1 is critical for the coactivator-induced
            allosteric transition that stimulates APC/C catalytic activity
  JOURNAL   Proc Natl Acad Sci U S A 113 (38), 10547-10552 (2016)
   PUBMED   27601667
  REMARK    GeneRIF: cryo-EM structure of an APC/C-Cdh1 complex with Apc1(WD40)
            deleted showed that the mutant APC/C is locked into an inactive
            conformation in which the UbcH10-binding site of the catalytic
            module is inaccessible. Additionally, an EM density for Apc15 is
            not visible
REFERENCE   4  (bases 1 to 1478)
  AUTHORS   Zhang,S., Chang,L., Alfieri,C., Zhang,Z., Yang,J., Maslen,S.,
            Skehel,M. and Barford,D.
  TITLE     Molecular mechanism of APC/C activation by mitotic phosphorylation
  JOURNAL   Nature 533 (7602), 260-264 (2016)
   PUBMED   27120157
REFERENCE   5  (bases 1 to 1478)
  AUTHORS   Uzunova,K., Dye,B.T., Schutz,H., Ladurner,R., Petzold,G.,
            Toyoda,Y., Jarvis,M.A., Brown,N.G., Poser,I., Novatchkova,M.,
            Mechtler,K., Hyman,A.A., Stark,H., Schulman,B.A. and Peters,J.M.
  TITLE     APC15 mediates CDC20 autoubiquitylation by APC/C(MCC) and
            disassembly of the mitotic checkpoint complex
  JOURNAL   Nat Struct Mol Biol 19 (11), 1116-1123 (2012)
   PUBMED   23007861
  REMARK    GeneRIF: APC15 is required for anaphase-promoting
            complex/cyclosome-bound mitotic checkpoint complex-dependent CDC20
            autoubiquitylation and degradation and for timely anaphase
            initiation.
REFERENCE   6  (bases 1 to 1478)
  AUTHORS   Mansfeld,J., Collin,P., Collins,M.O., Choudhary,J.S. and Pines,J.
  TITLE     APC15 drives the turnover of MCC-CDC20 to make the spindle assembly
            checkpoint responsive to kinetochore attachment
  JOURNAL   Nat Cell Biol 13 (10), 1234-1243 (2011)
   PUBMED   21926987
  REMARK    GeneRIF: Anaphase promoting complex subunit 15(APC15) mediates the
            constant turnover of CDC20 and mitotic checkpoint protein
            complexes, allowing the spindle checkpoint assembly to respond to
            the attachment state of kinetochores.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 1478)
  AUTHORS   Castro,A., Bernis,C., Vigneron,S., Labbe,J.C. and Lorca,T.
  TITLE     The anaphase-promoting complex: a key factor in the regulation of
            cell cycle
  JOURNAL   Oncogene 24 (3), 314-325 (2005)
   PUBMED   15678131
  REMARK    Review article
REFERENCE   8  (bases 1 to 1478)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AP000812.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK311146.1, DA137927.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1968189 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-46                AP000812.4         19536-19581         c
            47-106              AP000812.4         17430-17489         c
            107-244             AP000812.4         16974-17111         c
            245-1478            AP000812.4         13258-14491         c
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.4"
     gene            1..1478
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /note="anaphase promoting complex subunit 15"
                     /db_xref="GeneID:25906"
                     /db_xref="HGNC:HGNC:24531"
                     /db_xref="MIM:614717"
     exon            1..46
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /inference="alignment:Splign:2.1.0"
     exon            47..106
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /inference="alignment:Splign:2.1.0"
     exon            107..244
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /inference="alignment:Splign:2.1.0"
     CDS             182..499
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /note="isoform f is encoded by transcript variant 45"
                     /codon_start=1
                     /product="anaphase-promoting complex subunit 15 isoform f"
                     /protein_id="NP_001380388.1"
                     /db_xref="CCDS:CCDS91530.1"
                     /db_xref="GeneID:25906"
                     /db_xref="HGNC:HGNC:24531"
                     /db_xref="MIM:614717"
                     /translation="MQDMDEMNDYNESPDDGEVNEVHLSVLPTCGLYHSHPKQLCPES
                     IKLSHSFMPHVYMWLALPFPPPSPSAWPTQNFQIQFKCCSFSMKSQAETTTLSFRFMK
                     GLC"
     exon            245..1478
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1458..1463
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /note="hexamer: AATAAA"
     polyA_site      1478
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /note="major polyA site"
ORIGIN      
        1 gggtgaagcg cttccgggtc gccgccggca gcagcctccc ggcgcgctcc aaagcatcgc
       61 ggagaaagac aacaacctgg ttcctattgg caagccagcc tcagagcact atgatgacga
      121 ggaagaagag gatgatgaag atgatgagga tagtgaagag gactcagagg atgatgagga
      181 tatgcaggac atggacgaga tgaatgacta caatgagtca ccggatgatg gagaggtcaa
      241 tgaggttcat ctctcagtac ttcccacctg tggcctgtat cacagccatc ccaaacaact
      301 gtgcccagaa tccatcaagc tgtctcattc cttcatgcca catgtgtata tgtggctggc
      361 tttgcccttc ccacccccat cgccatctgc ctggccaact cagaacttcc agattcagtt
      421 caaatgttgc tctttctcca tgaagtccca ggcagaaaca accaccctat ctttcagatt
      481 tatgaaaggt ctctgttaga atttgtagtt tcattcccct tttattgctc atcaaatgta
      541 tttctgatct tggaattgga tgaactttta tttatttatt tttgagacca agtcttgctg
      601 tgttgcccag gctggagtgc agtagcatga tcacggctca ctgcagcctt gaccacccag
      661 gctcaggcaa tcctcccacc tcagcatttc cagtagctgg aaccacagtt actcaccacc
      721 acacccggct aatttttaaa ttttttgtag aaacgggggt cttgctttgt tacccaggct
      781 agtctcgaac tcctgggctc aagtgatcct cctgctttgg cctcccaaag tgctgggatt
      841 acaggcatga gccaccatgc ccagccagtg aatttctttt cttttctttt tctttttttt
      901 tttttttttg agacaggttc ttgctctgtc acccatgctg gagtgcagtg gcacaatcac
      961 agctcactgc agcctcagcc tcctgggctc aagcaatcct cccacctcag cctcccaagt
     1021 agctgggacc acaggcatgt gccaccatgc ctgggtaatt tttgtatttt ttgtagagat
     1081 gggtttttgc catgttgccc aagccggtct caaactcctg agctcaagca atctgcccac
     1141 ctcggcctct caaagtgctg ggattacagg caccagccac cacacagccg aatttcttaa
     1201 ataagaccct aaaagcactt atgctgggat tgagataaat ccaggcagac agctacccta
     1261 aatggtatgt ggaagcctcc atggtggaga ggaaagatgt ggagacagat aattacaaag
     1321 ctatgggtta tctgctgaga tggttattcc actgtgtatt atggttcctt tgaggccagc
     1381 atttgtggct cattcatctc tgtggcctct acccctctcc ctggcaccta gcacattcct
     1441 aatacaaaag aggtggcaat aaatgtttgc tgaataaa
//
