LOCUS       NM_001393431            1959 bp    mRNA    linear   PRI 03-MAY-2024
DEFINITION  Homo sapiens anaphase promoting complex subunit 15 (ANAPC15),
            transcript variant 17, mRNA.
ACCESSION   NM_001393431
VERSION     NM_001393431.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Yau,R.G., Doerner,K., Castellanos,E.R., Haakonsen,D.L., Werner,A.,
            Wang,N., Yang,X.W., Martinez-Martin,N., Matsumoto,M.L., Dixit,V.M.
            and Rape,M.
  TITLE     Assembly and Function of Heterotypic Ubiquitin Chains in Cell-Cycle
            and Protein Quality Control
  JOURNAL   Cell 171 (4), 918-933 (2017)
   PUBMED   29033132
REFERENCE   3  (bases 1 to 1959)
  AUTHORS   Li,Q., Chang,L., Aibara,S., Yang,J., Zhang,Z. and Barford,D.
  TITLE     WD40 domain of Apc1 is critical for the coactivator-induced
            allosteric transition that stimulates APC/C catalytic activity
  JOURNAL   Proc Natl Acad Sci U S A 113 (38), 10547-10552 (2016)
   PUBMED   27601667
  REMARK    GeneRIF: cryo-EM structure of an APC/C-Cdh1 complex with Apc1(WD40)
            deleted showed that the mutant APC/C is locked into an inactive
            conformation in which the UbcH10-binding site of the catalytic
            module is inaccessible. Additionally, an EM density for Apc15 is
            not visible
REFERENCE   4  (bases 1 to 1959)
  AUTHORS   Zhang,S., Chang,L., Alfieri,C., Zhang,Z., Yang,J., Maslen,S.,
            Skehel,M. and Barford,D.
  TITLE     Molecular mechanism of APC/C activation by mitotic phosphorylation
  JOURNAL   Nature 533 (7602), 260-264 (2016)
   PUBMED   27120157
REFERENCE   5  (bases 1 to 1959)
  AUTHORS   Uzunova,K., Dye,B.T., Schutz,H., Ladurner,R., Petzold,G.,
            Toyoda,Y., Jarvis,M.A., Brown,N.G., Poser,I., Novatchkova,M.,
            Mechtler,K., Hyman,A.A., Stark,H., Schulman,B.A. and Peters,J.M.
  TITLE     APC15 mediates CDC20 autoubiquitylation by APC/C(MCC) and
            disassembly of the mitotic checkpoint complex
  JOURNAL   Nat Struct Mol Biol 19 (11), 1116-1123 (2012)
   PUBMED   23007861
  REMARK    GeneRIF: APC15 is required for anaphase-promoting
            complex/cyclosome-bound mitotic checkpoint complex-dependent CDC20
            autoubiquitylation and degradation and for timely anaphase
            initiation.
REFERENCE   6  (bases 1 to 1959)
  AUTHORS   Mansfeld,J., Collin,P., Collins,M.O., Choudhary,J.S. and Pines,J.
  TITLE     APC15 drives the turnover of MCC-CDC20 to make the spindle assembly
            checkpoint responsive to kinetochore attachment
  JOURNAL   Nat Cell Biol 13 (10), 1234-1243 (2011)
   PUBMED   21926987
  REMARK    GeneRIF: Anaphase promoting complex subunit 15(APC15) mediates the
            constant turnover of CDC20 and mitotic checkpoint protein
            complexes, allowing the spindle checkpoint assembly to respond to
            the attachment state of kinetochores.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 1959)
  AUTHORS   Castro,A., Bernis,C., Vigneron,S., Labbe,J.C. and Lorca,T.
  TITLE     The anaphase-promoting complex: a key factor in the regulation of
            cell cycle
  JOURNAL   Oncogene 24 (3), 314-325 (2005)
   PUBMED   15678131
  REMARK    Review article
REFERENCE   8  (bases 1 to 1959)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AP000812.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.3673373.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1968189 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-312               AP000812.4         18810-19121         c
            313-397             AP000812.4         18298-18382         c
            398-527             AP000812.4         18043-18172         c
            528-587             AP000812.4         17430-17489         c
            588-725             AP000812.4         16974-17111         c
            726-1959            AP000812.4         13258-14491         c
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.4"
     gene            1..1959
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /note="anaphase promoting complex subunit 15"
                     /db_xref="GeneID:25906"
                     /db_xref="HGNC:HGNC:24531"
                     /db_xref="MIM:614717"
     exon            1..312
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /inference="alignment:Splign:2.1.0"
     exon            313..397
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /inference="alignment:Splign:2.1.0"
     exon            398..527
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /inference="alignment:Splign:2.1.0"
     CDS             408..980
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /note="isoform c is encoded by transcript variant 17"
                     /codon_start=1
                     /product="anaphase-promoting complex subunit 15 isoform c"
                     /protein_id="NP_001380360.1"
                     /db_xref="CCDS:CCDS81593.1"
                     /db_xref="GeneID:25906"
                     /db_xref="HGNC:HGNC:24531"
                     /db_xref="MIM:614717"
                     /translation="MSTLFPSLFPRVTETLWFNLDRPCVEETELQQQEQQHQAWLQSI
                     AEKDNNLVPIGKPASEHYDDEEEEDDEDDEDSEEDSEDDEDMQDMDEMNDYNESPDDG
                     EVNEVHLSVLPTCGLYHSHPKQLCPESIKLSHSFMPHVYMWLALPFPPPSPSAWPTQN
                     FQIQFKCCSFSMKSQAETTTLSFRFMKGLC"
     exon            528..587
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /inference="alignment:Splign:2.1.0"
     exon            588..725
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /inference="alignment:Splign:2.1.0"
     exon            726..1959
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1939..1944
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /note="hexamer: AATAAA"
     polyA_site      1959
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /note="major polyA site"
ORIGIN      
        1 agtacttctt ggacatgagg gtgtttaccg tcgtgcctaa ttacgcgggt tgggaatctg
       61 agatacaggg aagggcagcg gctggccctg ggtcggtcag ataaattaag acaaaagcag
      121 gaccaggcat cgattatttt ctcctgctca tccctttcac ggaggaattc taagccccga
      181 cacactgtat gacctaaagc cagtctctgc ctgtcgctgg gcctcagttc gcctgtccta
      241 agtttaattc agattatctg gcactcctgg tcggctccct cagccgctca aagctgattg
      301 tacccttata atatgaagac actgaggctc agagaggtta agtgactcag ccaaggtcaa
      361 acagctagta agtggtggag ccaggactca aagccagtct aggagccatg tccactttgt
      421 tcccctcact cttccctcgt gtgactgaga ctctgtggtt taatctggat cgaccctgtg
      481 tggaagagac agagctgcag cagcaggaac agcagcatca ggcctggctc caaagcatcg
      541 cggagaaaga caacaacctg gttcctattg gcaagccagc ctcagagcac tatgatgacg
      601 aggaagaaga ggatgatgaa gatgatgagg atagtgaaga ggactcagag gatgatgagg
      661 atatgcagga catggacgag atgaatgact acaatgagtc accggatgat ggagaggtca
      721 atgaggttca tctctcagta cttcccacct gtggcctgta tcacagccat cccaaacaac
      781 tgtgcccaga atccatcaag ctgtctcatt ccttcatgcc acatgtgtat atgtggctgg
      841 ctttgccctt cccaccccca tcgccatctg cctggccaac tcagaacttc cagattcagt
      901 tcaaatgttg ctctttctcc atgaagtccc aggcagaaac aaccacccta tctttcagat
      961 ttatgaaagg tctctgttag aatttgtagt ttcattcccc ttttattgct catcaaatgt
     1021 atttctgatc ttggaattgg atgaactttt atttatttat ttttgagacc aagtcttgct
     1081 gtgttgccca ggctggagtg cagtagcatg atcacggctc actgcagcct tgaccaccca
     1141 ggctcaggca atcctcccac ctcagcattt ccagtagctg gaaccacagt tactcaccac
     1201 cacacccggc taatttttaa attttttgta gaaacggggg tcttgctttg ttacccaggc
     1261 tagtctcgaa ctcctgggct caagtgatcc tcctgctttg gcctcccaaa gtgctgggat
     1321 tacaggcatg agccaccatg cccagccagt gaatttcttt tcttttcttt ttcttttttt
     1381 tttttttttt gagacaggtt cttgctctgt cacccatgct ggagtgcagt ggcacaatca
     1441 cagctcactg cagcctcagc ctcctgggct caagcaatcc tcccacctca gcctcccaag
     1501 tagctgggac cacaggcatg tgccaccatg cctgggtaat ttttgtattt tttgtagaga
     1561 tgggtttttg ccatgttgcc caagccggtc tcaaactcct gagctcaagc aatctgccca
     1621 cctcggcctc tcaaagtgct gggattacag gcaccagcca ccacacagcc gaatttctta
     1681 aataagaccc taaaagcact tatgctggga ttgagataaa tccaggcaga cagctaccct
     1741 aaatggtatg tggaagcctc catggtggag aggaaagatg tggagacaga taattacaaa
     1801 gctatgggtt atctgctgag atggttattc cactgtgtat tatggttcct ttgaggccag
     1861 catttgtggc tcattcatct ctgtggcctc tacccctctc cctggcacct agcacattcc
     1921 taatacaaaa gaggtggcaa taaatgtttg ctgaataaa
//
