LOCUS       NM_001393427            1688 bp    mRNA    linear   PRI 03-MAY-2024
DEFINITION  Homo sapiens anaphase promoting complex subunit 15 (ANAPC15),
            transcript variant 13, mRNA.
ACCESSION   NM_001393427
VERSION     NM_001393427.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Yau,R.G., Doerner,K., Castellanos,E.R., Haakonsen,D.L., Werner,A.,
            Wang,N., Yang,X.W., Martinez-Martin,N., Matsumoto,M.L., Dixit,V.M.
            and Rape,M.
  TITLE     Assembly and Function of Heterotypic Ubiquitin Chains in Cell-Cycle
            and Protein Quality Control
  JOURNAL   Cell 171 (4), 918-933 (2017)
   PUBMED   29033132
REFERENCE   3  (bases 1 to 1688)
  AUTHORS   Li,Q., Chang,L., Aibara,S., Yang,J., Zhang,Z. and Barford,D.
  TITLE     WD40 domain of Apc1 is critical for the coactivator-induced
            allosteric transition that stimulates APC/C catalytic activity
  JOURNAL   Proc Natl Acad Sci U S A 113 (38), 10547-10552 (2016)
   PUBMED   27601667
  REMARK    GeneRIF: cryo-EM structure of an APC/C-Cdh1 complex with Apc1(WD40)
            deleted showed that the mutant APC/C is locked into an inactive
            conformation in which the UbcH10-binding site of the catalytic
            module is inaccessible. Additionally, an EM density for Apc15 is
            not visible
REFERENCE   4  (bases 1 to 1688)
  AUTHORS   Zhang,S., Chang,L., Alfieri,C., Zhang,Z., Yang,J., Maslen,S.,
            Skehel,M. and Barford,D.
  TITLE     Molecular mechanism of APC/C activation by mitotic phosphorylation
  JOURNAL   Nature 533 (7602), 260-264 (2016)
   PUBMED   27120157
REFERENCE   5  (bases 1 to 1688)
  AUTHORS   Uzunova,K., Dye,B.T., Schutz,H., Ladurner,R., Petzold,G.,
            Toyoda,Y., Jarvis,M.A., Brown,N.G., Poser,I., Novatchkova,M.,
            Mechtler,K., Hyman,A.A., Stark,H., Schulman,B.A. and Peters,J.M.
  TITLE     APC15 mediates CDC20 autoubiquitylation by APC/C(MCC) and
            disassembly of the mitotic checkpoint complex
  JOURNAL   Nat Struct Mol Biol 19 (11), 1116-1123 (2012)
   PUBMED   23007861
  REMARK    GeneRIF: APC15 is required for anaphase-promoting
            complex/cyclosome-bound mitotic checkpoint complex-dependent CDC20
            autoubiquitylation and degradation and for timely anaphase
            initiation.
REFERENCE   6  (bases 1 to 1688)
  AUTHORS   Mansfeld,J., Collin,P., Collins,M.O., Choudhary,J.S. and Pines,J.
  TITLE     APC15 drives the turnover of MCC-CDC20 to make the spindle assembly
            checkpoint responsive to kinetochore attachment
  JOURNAL   Nat Cell Biol 13 (10), 1234-1243 (2011)
   PUBMED   21926987
  REMARK    GeneRIF: Anaphase promoting complex subunit 15(APC15) mediates the
            constant turnover of CDC20 and mitotic checkpoint protein
            complexes, allowing the spindle checkpoint assembly to respond to
            the attachment state of kinetochores.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 1688)
  AUTHORS   Castro,A., Bernis,C., Vigneron,S., Labbe,J.C. and Lorca,T.
  TITLE     The anaphase-promoting complex: a key factor in the regulation of
            cell cycle
  JOURNAL   Oncogene 24 (3), 314-325 (2005)
   PUBMED   15678131
  REMARK    Review article
REFERENCE   8  (bases 1 to 1688)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AP000812.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038196.525964.1,
                                           SRR14478891.343159.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-46                AP000812.4         19536-19581         c
            47-131              AP000812.4         18298-18382         c
            132-256             AP000812.4         18043-18167         c
            257-316             AP000812.4         17430-17489         c
            317-454             AP000812.4         16974-17111         c
            455-1688            AP000812.4         13258-14491         c
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.4"
     gene            1..1688
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /note="anaphase promoting complex subunit 15"
                     /db_xref="GeneID:25906"
                     /db_xref="HGNC:HGNC:24531"
                     /db_xref="MIM:614717"
     exon            1..46
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /inference="alignment:Splign:2.1.0"
     exon            47..131
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /inference="alignment:Splign:2.1.0"
     exon            132..256
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /inference="alignment:Splign:2.1.0"
     CDS             137..709
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /note="isoform c is encoded by transcript variant 13"
                     /codon_start=1
                     /product="anaphase-promoting complex subunit 15 isoform c"
                     /protein_id="NP_001380356.1"
                     /db_xref="CCDS:CCDS81593.1"
                     /db_xref="GeneID:25906"
                     /db_xref="HGNC:HGNC:24531"
                     /db_xref="MIM:614717"
                     /translation="MSTLFPSLFPRVTETLWFNLDRPCVEETELQQQEQQHQAWLQSI
                     AEKDNNLVPIGKPASEHYDDEEEEDDEDDEDSEEDSEDDEDMQDMDEMNDYNESPDDG
                     EVNEVHLSVLPTCGLYHSHPKQLCPESIKLSHSFMPHVYMWLALPFPPPSPSAWPTQN
                     FQIQFKCCSFSMKSQAETTTLSFRFMKGLC"
     exon            257..316
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /inference="alignment:Splign:2.1.0"
     exon            317..454
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /inference="alignment:Splign:2.1.0"
     exon            455..1688
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1668..1673
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /note="hexamer: AATAAA"
     polyA_site      1688
                     /gene="ANAPC15"
                     /gene_synonym="APC15; C11orf51; HSPC020"
                     /note="major polyA site"
ORIGIN      
        1 gggtgaagcg cttccgggtc gccgccggca gcagcctccc ggcgcgatga agacactgag
       61 gctcagagag gttaagtgac tcagccaagg tcaaacagct agtaagtggt ggagccagga
      121 ctcaaagcca ggagccatgt ccactttgtt cccctcactc ttccctcgtg tgactgagac
      181 tctgtggttt aatctggatc gaccctgtgt ggaagagaca gagctgcagc agcaggaaca
      241 gcagcatcag gcctggctcc aaagcatcgc ggagaaagac aacaacctgg ttcctattgg
      301 caagccagcc tcagagcact atgatgacga ggaagaagag gatgatgaag atgatgagga
      361 tagtgaagag gactcagagg atgatgagga tatgcaggac atggacgaga tgaatgacta
      421 caatgagtca ccggatgatg gagaggtcaa tgaggttcat ctctcagtac ttcccacctg
      481 tggcctgtat cacagccatc ccaaacaact gtgcccagaa tccatcaagc tgtctcattc
      541 cttcatgcca catgtgtata tgtggctggc tttgcccttc ccacccccat cgccatctgc
      601 ctggccaact cagaacttcc agattcagtt caaatgttgc tctttctcca tgaagtccca
      661 ggcagaaaca accaccctat ctttcagatt tatgaaaggt ctctgttaga atttgtagtt
      721 tcattcccct tttattgctc atcaaatgta tttctgatct tggaattgga tgaactttta
      781 tttatttatt tttgagacca agtcttgctg tgttgcccag gctggagtgc agtagcatga
      841 tcacggctca ctgcagcctt gaccacccag gctcaggcaa tcctcccacc tcagcatttc
      901 cagtagctgg aaccacagtt actcaccacc acacccggct aatttttaaa ttttttgtag
      961 aaacgggggt cttgctttgt tacccaggct agtctcgaac tcctgggctc aagtgatcct
     1021 cctgctttgg cctcccaaag tgctgggatt acaggcatga gccaccatgc ccagccagtg
     1081 aatttctttt cttttctttt tctttttttt tttttttttg agacaggttc ttgctctgtc
     1141 acccatgctg gagtgcagtg gcacaatcac agctcactgc agcctcagcc tcctgggctc
     1201 aagcaatcct cccacctcag cctcccaagt agctgggacc acaggcatgt gccaccatgc
     1261 ctgggtaatt tttgtatttt ttgtagagat gggtttttgc catgttgccc aagccggtct
     1321 caaactcctg agctcaagca atctgcccac ctcggcctct caaagtgctg ggattacagg
     1381 caccagccac cacacagccg aatttcttaa ataagaccct aaaagcactt atgctgggat
     1441 tgagataaat ccaggcagac agctacccta aatggtatgt ggaagcctcc atggtggaga
     1501 ggaaagatgt ggagacagat aattacaaag ctatgggtta tctgctgaga tggttattcc
     1561 actgtgtatt atggttcctt tgaggccagc atttgtggct cattcatctc tgtggcctct
     1621 acccctctcc ctggcaccta gcacattcct aatacaaaag aggtggcaat aaatgtttgc
     1681 tgaataaa
//
