LOCUS       NM_022662               7762 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens anaphase promoting complex subunit 1 (ANAPC1), mRNA.
ACCESSION   NM_022662 XM_938503
VERSION     NM_022662.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7762)
  AUTHORS   Mann,G., Sulkshane,P., Sadhu,P., Ziv,T., Glickman,M.H. and Brik,A.
  TITLE     Antibody for Serine 65 Phosphorylated Ubiquitin Identifies
            PLK1-Mediated Phosphorylation of Mitotic Proteins and APC1
  JOURNAL   Molecules 27 (15), 4867 (2022)
   PUBMED   35956818
  REMARK    GeneRIF: Antibody for Serine 65 Phosphorylated Ubiquitin Identifies
            PLK1-Mediated Phosphorylation of Mitotic Proteins and APC1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7762)
  AUTHORS   Liess,A.K.L., Kucerova,A., Schweimer,K., Schlesinger,D., Dybkov,O.,
            Urlaub,H., Mansfeld,J. and Lorenz,S.
  TITLE     Dimerization regulates the human APC/C-associated
            ubiquitin-conjugating enzyme UBE2S
  JOURNAL   Sci Signal 13 (654) (2020)
   PUBMED   33082289
  REMARK    GeneRIF: Dimerization regulates the human APC/C-associated
            ubiquitin-conjugating enzyme UBE2S.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7762)
  AUTHORS   Ajeawung,N.F., Nguyen,T.T.M., Lu,L., Kucharski,T.J., Rousseau,J.,
            Molidperee,S., Atienza,J., Gamache,I., Jin,W., Plon,S.E., Lee,B.H.,
            Teodoro,J.G., Wang,L.L. and Campeau,P.M.
  TITLE     Mutations in ANAPC1, Encoding a Scaffold Subunit of the
            Anaphase-Promoting Complex, Cause Rothmund-Thomson Syndrome Type 1
  JOURNAL   Am J Hum Genet 105 (3), 625-630 (2019)
   PUBMED   31303264
  REMARK    GeneRIF: Fibroblast studies showed that the intronic mutation
            causes the activation of a 95 bp pseudoexon, leading to mRNAs with
            premature termination codons and nonsense-mediated decay, decreased
            ANAPC1 protein levels, and prolongation of interphase
REFERENCE   4  (bases 1 to 7762)
  AUTHORS   Ivarsdottir,E.V., Benonisdottir,S., Thorleifsson,G., Sulem,P.,
            Oddsson,A., Styrkarsdottir,U., Kristmundsdottir,S.,
            Arnadottir,G.A., Thorgeirsson,G., Jonsdottir,I., Zoega,G.M.,
            Thorsteinsdottir,U., Gudbjartsson,D.F., Jonasson,F., Holm,H. and
            Stefansson,K.
  TITLE     Sequence variation at ANAPC1 accounts for 24% of the variability in
            corneal endothelial cell density
  JOURNAL   Nat Commun 10 (1), 1284 (2019)
   PUBMED   30894546
  REMARK    GeneRIF: Sequence variation at ANAPC1 accounts for 24% of the
            variability in corneal endothelial cell density.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7762)
  AUTHORS   Watson,E.R., Brown,N.G., Peters,J.M., Stark,H. and Schulman,B.A.
  TITLE     Posing the APC/C E3 Ubiquitin Ligase to Orchestrate Cell Division
  JOURNAL   Trends Cell Biol 29 (2), 117-134 (2019)
   PUBMED   30482618
  REMARK    GeneRIF: The anaphase promoting complex/cyclosome (APC/C) E3 ligase
            is not simply an interaction hub, but a dynamic, multifunctional
            molecular machine whose structure is remodeled by binding partners
            to achieve temporal ubiquitylation regulating cell division
            [Review].
            Review article
REFERENCE   6  (bases 1 to 7762)
  AUTHORS   Kramer,E.R., Scheuringer,N., Podtelejnikov,A.V., Mann,M. and
            Peters,J.M.
  TITLE     Mitotic regulation of the APC activator proteins CDC20 and CDH1
  JOURNAL   Mol Biol Cell 11 (5), 1555-1569 (2000)
   PUBMED   10793135
REFERENCE   7  (bases 1 to 7762)
  AUTHORS   Lukas,C., Sorensen,C.S., Kramer,E., Santoni-Rugiu,E., Lindeneg,C.,
            Peters,J.M., Bartek,J. and Lukas,J.
  TITLE     Accumulation of cyclin B1 requires E2F and cyclin-A-dependent
            rearrangement of the anaphase-promoting complex
  JOURNAL   Nature 401 (6755), 815-818 (1999)
   PUBMED   10548110
REFERENCE   8  (bases 1 to 7762)
  AUTHORS   Gieffers,C., Peters,B.H., Kramer,E.R., Dotti,C.G. and Peters,J.M.
  TITLE     Expression of the CDH1-associated form of the anaphase-promoting
            complex in postmitotic neurons
  JOURNAL   Proc Natl Acad Sci U S A 96 (20), 11317-11322 (1999)
   PUBMED   10500174
REFERENCE   9  (bases 1 to 7762)
  AUTHORS   Grossberger,R., Gieffers,C., Zachariae,W., Podtelejnikov,A.V.,
            Schleiffer,A., Nasmyth,K., Mann,M. and Peters,J.M.
  TITLE     Characterization of the DOC1/APC10 subunit of the yeast and the
            human anaphase-promoting complex
  JOURNAL   J Biol Chem 274 (20), 14500-14507 (1999)
   PUBMED   10318877
REFERENCE   10 (bases 1 to 7762)
  AUTHORS   Wang,L.L. and Plon,S.E.
  TITLE     Rothmund-Thomson Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301415
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC104651.2 and AC093166.6.
            
            On Nov 23, 2018 this sequence version replaced NM_022662.3.
            
            Summary: This gene encodes a subunit of the anaphase-promoting
            complex. This complex is an E3 ubiquitin ligase that regulates
            progression through the metaphase to anaphase portion of the cell
            cycle by ubiquitinating proteins which targets them for
            degradation. [provided by RefSeq, Dec 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074967.1049856.1,
                                           SRR14038193.2038024.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000341068.8/ ENSP00000339109.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-252               AC104651.2         1536-1787           c
            253-489             AC093166.6         210072-210308       c
            490-651             AC093166.6         208269-208430       c
            652-703             AC093166.6         203072-203123       c
            704-804             AC093166.6         202767-202867       c
            805-887             AC093166.6         202089-202171       c
            888-961             AC093166.6         197482-197555       c
            962-1107            AC093166.6         194265-194410       c
            1108-1328           AC093166.6         193134-193354       c
            1329-1538           AC093166.6         191848-192057       c
            1539-1634           AC093166.6         187765-187860       c
            1635-1725           AC093166.6         186255-186345       c
            1726-1791           AC093166.6         186073-186138       c
            1792-1926           AC093166.6         180235-180369       c
            1927-2067           AC093166.6         177184-177324       c
            2068-2128           AC093166.6         176597-176657       c
            2129-2316           AC093166.6         172871-173058       c
            2317-2391           AC093166.6         167897-167971       c
            2392-2660           AC093166.6         164063-164331       c
            2661-2752           AC093166.6         162679-162770       c
            2753-2901           AC093166.6         160745-160893       c
            2902-2980           AC093166.6         155236-155314       c
            2981-3017           AC093166.6         154590-154626       c
            3018-3088           AC093166.6         154425-154495       c
            3089-3267           AC093166.6         151881-152059       c
            3268-3482           AC093166.6         150698-150912       c
            3483-3601           AC093166.6         148299-148417       c
            3602-3873           AC093166.6         144829-145100       c
            3874-4108           AC093166.6         138406-138640       c
            4109-4200           AC093166.6         135261-135352       c
            4201-4335           AC093166.6         133164-133298       c
            4336-4418           AC093166.6         132907-132989       c
            4419-4497           AC093166.6         131887-131965       c
            4498-4572           AC093166.6         130256-130330       c
            4573-4649           AC093166.6         124277-124353       c
            4650-4740           AC093166.6         123692-123782       c
            4741-4794           AC093166.6         123537-123590       c
            4795-4988           AC093166.6         121821-122014       c
            4989-5075           AC093166.6         117693-117779       c
            5076-5193           AC093166.6         114819-114936       c
            5194-5271           AC093166.6         113782-113859       c
            5272-5339           AC093166.6         113356-113423       c
            5340-5478           AC093166.6         111828-111966       c
            5479-5565           AC093166.6         109758-109844       c
            5566-5661           AC093166.6         108134-108229       c
            5662-5860           AC093166.6         106318-106516       c
            5861-5995           AC093166.6         101800-101934       c
            5996-7762           AC093166.6         97099-98865         c
FEATURES             Location/Qualifiers
     source          1..7762
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q13"
     gene            1..7762
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /note="anaphase promoting complex subunit 1"
                     /db_xref="GeneID:64682"
                     /db_xref="HGNC:HGNC:19988"
                     /db_xref="MIM:608473"
     exon            1..252
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            253..489
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     CDS             277..6111
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /note="anaphase-promoting complex 1 (meiotic checkpoint
                     regulator); cyclosome subunit 1; mitotic checkpoint
                     regulator; testis-specific gene 24 protein"
                     /codon_start=1
                     /product="anaphase-promoting complex subunit 1"
                     /protein_id="NP_073153.1"
                     /db_xref="CCDS:CCDS2093.1"
                     /db_xref="GeneID:64682"
                     /db_xref="HGNC:HGNC:19988"
                     /db_xref="MIM:608473"
                     /translation="MSNFYEERTTMIAARDLQEFVPFGRDHCKHHPNALNLQLRQLQP
                     ASELWSSDGAAGLVGSLQEVTIHEKQKESWQLRKGVSEIGEDVDYDEELYVAGNMVIW
                     SKGSKSQALAVYKAFTVDSPVQQALWCDFIISQDKSEKAYSSNEVEKCICILQSSCIN
                     MHSIEGKDYIASLPFQVANVWPTKYGLLFERSASSHEVPPGSPREPLPTMFSMLHPLD
                     EITPLVCKSGSLFGSSRVQYVVDHAMKIVFLNTDPSIVMTYDAVQNVHSVWTLRRVKS
                     EEENVVLKFSEQGGTPQNVATSSSLTAHLRSLSKGDSPVTSPFQNYSSIHSQSRSTSS
                     PSLHSRSPSISNMAALSRAHSPALGVHSFSGVQRFNISSHNQSPKRHSISHSPNSNSN
                     GSFLAPETEPIVPELCIDHLWTETITNIREKNSQASKVFITSDLCGQKFLCFLVESQL
                     QLRCVKFQESNDKTQLIFGSVTNIPAKDAAPVEKIDTMLVLEGSGNLVLYTGVVRVGK
                     VFIPGLPAPSLTMSNTMPRPSTPLDGVSTPKPLSKLLGSLDEVVLLSPVPELRDSSKL
                     HDSLYNEDCTFQQLGTYIHSIRDPVHNRVTLELSNGSMVRITIPEIATSELVQTCLQA
                     IKFILPKEIAVQMLVKWYNVHSAPGGPSYHSEWNLFVTCLMNMMGYNTDRLAWTRNFD
                     FEGSLSPVIAPKKARPSETGSDDDWEYLLNSDYHQNVESHLLNRSLCLSPSEASQMKD
                     EDFSQNLSLDSSTLLFTHIPAIFFVLHLVYEELKLNTLMGEGICSLVELLVQLARDLK
                     LGPYVDHYYRDYPTLVRTTGQVCTIDPGQTGFMHHPSFFTSEPPSIYQWVSSCLKGEG
                     MPPYPYLPGICERSRLVVLSIALYILGDESLVSDESSQYLTRITIAPQKLQVEQEENR
                     FSFRHSTSVSSLAERLVVWMTNVGFTLRDLETLPFGIALPIRDAIYHCREQPASDWPE
                     AVCLLIGRQDLSKQACEGNLPKGKSVLSSDVPSGTETEEEDDGMNDMNHEVMSLIWSE
                     DLRVQDVRRLLQSAHPVRVNVVQYPELSDHEFIEEKENRLLQLCQRTMALPVGRGMFT
                     LFSYHPVPTEPLPIPKLNLTGRAPPRNTTVDLNSGNIDVPPNMTSWASFHNGVAAGLK
                     IAPASQIDSAWIVYNKPKHAELANEYAGFLMALGLNGHLTKLATLNIHDYLTKGHEMT
                     SIGLLLGVSAAKLGTMDMSITRLLSIHIPALLPPTSTELDVPHNVQVAAVVGIGLVYQ
                     GTAHRHTAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLN
                     VPEQLYQYMVGGHRRFQTGMHREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKT
                     NNRSIADWLRAPDTMYLLDFVKPEFLLLRTLARCLILWDDILPNSKWVDSNVPQIIRE
                     NSISLSEIELPCSEDLNLETLSQAHVYIIAGACLSLGFRFAGSENLSAFNCLHKFAKD
                     FMTYLSAPNASVTGPHNLETCLSVVLLSLAMVMAGSGNLKVLQLCRFLHMKTGGEMNY
                     GFHLAHHMALGLLFLGGGRYSLSTSNSSIAALLCALYPHFPAHSTDNRYHLQALRHLY
                     VLAAEPRLLVPVDVDTNTPCYALLEVTYKGTQWYEQTKEELMAPTLLPELHLLKQIKV
                     KGPRYWELLIDLSKGTQHLKSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVANR
                     NSEARAFKPETISAFTSDPALLSFAEYFCKPTVNMGQKQEILDLFSSVLYECVTQETP
                     EMLPAYIAMDQAIRRLGRREMSETSELWQIKLVLEFFSSRSHQERLQNHPKRGLFMNS
                     EFLPVVKCTIDNTLDQWLQVGGDMCVHAYLSGQPLEESQLSMLACFLVYHSVPAPQHL
                     PPIGLEGSTSFAELLFKFKQLKMPVRALLRLAPLLLGNPQPMVM"
     exon            490..651
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            652..703
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            704..804
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            805..887
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            888..961
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1107
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1328
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..1538
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            1539..1634
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            1635..1725
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            1726..1791
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            1792..1926
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            1927..2067
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            2068..2128
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            2129..2316
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            2317..2391
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            2392..2660
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            2661..2752
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            2753..2901
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            2902..2980
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            2981..3017
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            3018..3088
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            3089..3267
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            3268..3482
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            3483..3601
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            3602..3873
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            3874..4108
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            4109..4200
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            4201..4335
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            4336..4418
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            4419..4497
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            4498..4572
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            4573..4649
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            4650..4740
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            4741..4794
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            4795..4988
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            4989..5075
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            5076..5193
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            5194..5271
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            5272..5339
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            5340..5478
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            5479..5565
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            5566..5661
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            5662..5860
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            5861..5995
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     exon            5996..7762
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7740..7745
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /note="hexamer: AATAAA"
     polyA_site      7762
                     /gene="ANAPC1"
                     /gene_synonym="APC1; MCPR; TSG24"
                     /note="major polyA site"
ORIGIN      
        1 gcgaggcaag acgggaaagt caagatggac gcgtccattt gaacgtctcg cacgccttcc
       61 tgccattagc actcgagccc gctgctgttg cccgttcttc ctccagaata ggggagggag
      121 agggaatgag aagctgctgc ggcccaagag tcactgtgaa ggaccccgcc gctgccctcg
      181 ggcctcctcg gcccctgcgc ctccggggag cagccggggc tcgccgcgcc tgacgcgtcc
      241 cgagttatac agaaataatg ttgatatttg gaacccatgt cgaacttcta tgaagaaagg
      301 acaacgatga ttgcagcaag ggatttgcag gaatttgttc cttttggtcg agaccactgc
      361 aagcaccacc ctaatgcttt gaaccttcaa cttcgccagc tgcagccagc ttctgaatta
      421 tggtcttctg atggtgctgc tggcttggtg ggatcccttc aggaggttac aatccacgag
      481 aaacagaagg aaagctggca gttaaggaaa ggagtaagtg aaattggaga agatgtggac
      541 tatgatgagg aactctatgt tgctggaaat atggtgatat ggagcaaagg aagtaaaagc
      601 caggcattgg cagtttataa agcatttaca gttgacagtc ctgttcagca ggcattgtgg
      661 tgtgacttca ttatatcaca ggataagtct gaaaaggcct acagtagcaa tgaagtagaa
      721 aaatgcatat gtatattgca aagctcatgt attaacatgc atagcataga aggaaaggat
      781 tacatagctt cattaccatt tcaggttgca aatgtttggc ccactaaata tggattgctg
      841 tttgaacgaa gcgcttcttc acatgaagta cctccaggtt cacccagaga acctttacct
      901 actatgttca gcatgctgca cccactagat gaaataactc cacttgtttg taaatctgga
      961 agtctttttg gttcatcacg ggtgcaatat gttgtagatc atgcaatgaa aattgttttc
     1021 ctcaatactg acccctctat tgtaatgact tatgatgctg ttcaaaatgt gcattctgtg
     1081 tggactctcc ggagagtcaa atcagaggaa gagaatgttg ttttaaagtt ctctgaacag
     1141 gggggaaccc cacagaatgt ggccactagc agctccctca cagcacatct cagaagcctc
     1201 tccaaaggag attcccctgt gacttcacct ttccagaatt actcctccat tcacagccag
     1261 agtcgctcaa cctcatcacc cagtctacat tctcgctcac cttctatttc caacatggca
     1321 gctctaagtc gtgctcattc tcctgcgtta ggagtgcact ctttttcagg ggtgcaaagg
     1381 ttcaacattt caagccataa tcagtctcca aagagacata gtatttctca ttctccaaat
     1441 agtaattcta atggctcctt tcttgcacca gaaacggagc caattgttcc tgaactgtgt
     1501 attgaccatt tgtggacaga aacgattact aatataagag agaaaaattc acaagcctca
     1561 aaagtgttta ttacatctga cctatgtggg caaaagttcc tgtgcttttt agtagagtcc
     1621 cagctccagt tacgctgtgt aaagtttcaa gagagtaatg ataaaaccca gctcatcttt
     1681 ggttcagtga ccaacatacc agcaaaggat gcagcaccag tggagaaaat agacaccatg
     1741 ctggtcttgg aaggcagtgg aaacctggtg ctatacacag gagtggttcg ggtgggaaag
     1801 gtttttattc ctggactgcc agctccctct ctgacgatgt ccaacacaat gcctcggccc
     1861 agtactccac tagatggcgt tagtactcca aagcctctta gtaaactcct tggatcattg
     1921 gacgaggttg ttctgttgtc cccagttcca gaactgaggg attcttcaaa acttcatgat
     1981 tctctctata atgaggattg tactttccaa cagcttggaa cttacattca ttctatcaga
     2041 gatcctgtcc ataacagagt caccctggaa ctgagtaatg gctccatggt taggatcact
     2101 attcctgaaa ttgccacctc tgagttagta caaacgtgtt tgcaagcaat taagtttatc
     2161 ctgccaaaag aaatagcagt tcagatgctt gtcaagtggt acaatgtcca cagtgctcca
     2221 ggaggaccca gttatcactc agagtggaat ttatttgtga cttgtctcat gaacatgatg
     2281 ggttataaca cagaccgctt agcatggact agaaattttg actttgaagg atcactttct
     2341 cctgtcattg cgcccaaaaa agcaaggcct tccgagactg gatctgatga tgactgggaa
     2401 tatttactaa attcagacta ccaccagaat gttgagtctc atcttttgaa cagatcttta
     2461 tgtctgagtc cttcagaagc ttcacagatg aaggatgagg atttttcaca gaatctcagt
     2521 ctggattctt ctacacttct ctttactcac atacctgcaa tttttttcgt tcttcacctt
     2581 gtgtatgagg agcttaagtt gaatactcta atgggagaag gaatttgttc acttgttgaa
     2641 cttctcgttc agttggcaag ggacttaaaa ttggggcctt atgtagatca ttactataga
     2701 gactacccaa cgcttgtcag aactactgga caagtgtgca caattgatcc aggtcaaaca
     2761 ggatttatgc atcatccatc attttttacg tctgagccac caagtattta tcagtgggtg
     2821 agttcttgtc tgaagggtga aggaatgcca ccttatcctt acctccctgg aatctgtgaa
     2881 agaagcagac ttgtagtctt gagtattgca ctgtacatac ttggtgatga gagcttggtt
     2941 tctgatgaat cctcacagta tttaaccaga ataactatag ccccccagaa gttgcaagta
     3001 gaacaagagg aaaacaggtt tagtttcagg cattctacat ctgtttctag tctagctgaa
     3061 agattggttg tctggatgac taatgtagga ttcactttaa gagatttgga aactcttccc
     3121 tttggaattg ctcttcccat cagagatgca atttatcact gtcgtgagca gcctgcctca
     3181 gactggccag aagctgtctg tctcttgatt ggacgtcagg atctttccaa gcaggcctgc
     3241 gaaggaaact tacccaaagg gaagtctgtg ctctcatcag atgttccttc aggaacagaa
     3301 actgaggagg aagatgacgg catgaatgac atgaatcacg aggtcatgtc attaatatgg
     3361 agtgaagatt taagggtgca ggatgtgcga aggcttcttc agagtgcgca tcctgtccgt
     3421 gtcaacgtag tgcagtaccc agagctcagt gaccacgagt tcatcgagga aaaggaaaac
     3481 agattgctcc aattgtgtca gcgaactatg gctcttcctg taggacgagg aatgtttacc
     3541 ttgttttcgt accatcctgt tccaacagag ccattgccta ttcctaaatt gaatctgact
     3601 gggcgtgccc ctcctcggaa cacaacagta gaccttaata gtggaaacat cgatgtgcct
     3661 cccaacatga caagctgggc cagctttcat aatggtgtgg ctgctggcct gaagatagct
     3721 cctgcctccc agatcgactc agcttggatt gtttacaata agcccaagca tgctgagttg
     3781 gccaatgagt atgctggctt tctcatggct ctgggtttga atgggcacct taccaagctg
     3841 gcgactctca atatccatga ctacttgacc aagggccatg aaatgacaag cattggactg
     3901 ctacttggtg tttctgctgc aaaactaggc accatggata tgtctattac tcggcttctt
     3961 agcattcaca ttcctgctct cttaccccca acgtccacag agctggatgt tcctcacaat
     4021 gtccaagtgg ctgcagtggt tggcattggc cttgtatatc aagggacagc tcacagacat
     4081 actgcagaag tcctgttggc tgagatagga cggcctcctg gtcctgaaat ggaatactgc
     4141 actgacagag agtcatactc cttagctgct ggcttggccc tgggcatggt ctgcttgggg
     4201 catggcagca atttgatagg tatgtctgat ctcaatgtgc ctgagcagct ctatcagtac
     4261 atggttggag gacataggcg ctttcaaaca ggaatgcata gggagaaaca taaatcacca
     4321 agttatcaaa tcaaagaagg agataccata aatgtggatg tgacttgtcc aggtgctact
     4381 ctagctttgg ctatgatcta cttaaaaacc aataacagat ctattgcaga ttggctccga
     4441 gcccctgaca ccatgtattt gttggacttt gtgaagccag aatttctctt gcttaggaca
     4501 cttgctcgat gcctgatttt gtgggatgat attttaccaa attccaagtg ggttgacagc
     4561 aatgttcctc aaattataag agaaaatagt atctctctca gtgaaatcga attgccgtgc
     4621 tcagaggatt tgaatttgga aactttgtcc caagcacatg tctacataat tgcaggagcc
     4681 tgcttgtctc tgggttttcg atttgctggc tcagaaaact tatcagcatt taactgtttg
     4741 cataaatttg ccaaagattt tatgacttat ttgtccgcac ctaatgcttc tgttacaggt
     4801 cctcataacc tagaaacttg tctgagcgtg gtgctgctgt ctctcgccat ggtcatggct
     4861 ggctcaggaa acctaaaggt tttgcagctt tgtcgcttct tacacatgaa aacgggtggt
     4921 gaaatgaact atggttttca cttagcccac cacatggccc ttggacttct atttttggga
     4981 ggaggaaggt actctttgag cacatcaaat tcttccattg ccgctcttct ctgtgccctt
     5041 tatccgcact tcccagctca cagcactgac aaccggtatc atctccaggc tctccggcac
     5101 ctctatgtgc tggccgcgga gcccaggctt ctagtgcctg tggatgtgga cacaaacacg
     5161 ccctgctatg ccctcttaga agttacctac aagggcactc agtggtatga acaaaccaaa
     5221 gaagaattga tggctcctac ccttcttcca gaactccatc ttttaaagca gattaaagta
     5281 aaaggcccaa gatactggga actgctcata gatttaagca aaggaacaca acacttgaag
     5341 tccatccttt ccaaggatgg ggttttatat gttaaactcc gggcgggtca gctctcctac
     5401 aaagaagatc caatgggatg gcaaagtttg ttggctcaga ctgttgctaa caggaactct
     5461 gaagcccggg ctttcaagcc agaaacaatc tcagcattca cttctgatcc agcacttctg
     5521 tcatttgctg aatatttctg caagccaact gtgaacatgg gtcagaaaca ggaaattctg
     5581 gatctctttt cttcagtact ctatgaatgt gttacccagg agaccccaga gatgttgcct
     5641 gcatacatcg caatggatca ggctataaga agacttggga gaagagaaat gtctgagact
     5701 tctgaacttt ggcagataaa gttggtgtta gagtttttca gctcccgaag ccatcaggag
     5761 cggctgcaga accaccctaa gcgggggctc tttatgaact cggaattcct ccctgttgtg
     5821 aagtgcacca ttgataatac cctggaccag tggctacaag tcgggggtga tatgtgtgtg
     5881 cacgcctacc tcagcgggca gcccttggag gaatcacagc tgagcatgct ggcctgcttc
     5941 ctcgtctacc actctgtgcc agctccacag cacctgccac ctataggact agaagggagc
     6001 acaagctttg ctgaactgct cttcaaattt aagcagctaa aaatgccagt gcgagctttg
     6061 ctgagattgg ctcctttgct tcttggaaat ccacagccaa tggtgatgtg accgtgtctg
     6121 gcggtgaacc taccctgaaa cgtgacttct gcacaacaaa cgtgaccaaa catcaaagct
     6181 aaagcaatgt ttataaagtt ttatggtata actaggggga aatgagctgc acaaacctca
     6241 atgtatttta aatctgttgc tgtcatcatt aacggtatat gacatataaa agcaagttaa
     6301 aatttacttt tgtaaataaa gtttttggtt tgtttccaaa actcttgatg attgctttag
     6361 ttttggactt agagaataga gcaggggttg ctggagtgaa tattgatttt taaagtcttt
     6421 gaactgtggt ggtataggtg aagtgactat gcccaaaaat gccaagtttt aaaagaagct
     6481 atgtcataaa gttttacttt ctgtggcaaa agagcgcttt agcatttcct cagatgtcac
     6541 agttgtcctg tctaaaataa gtttgtactt ctggtgacaa tgccagacac tcttatgatg
     6601 tgatcacctt aaacaggaga aggagatttt tgccctcagt tgctcaacat gaagtactgt
     6661 ggactgaatt gtgtcctccc agaattatat attgaggccc taatccctgt gtgactgtat
     6721 taggcaatag ggcatatagg agattactaa ggttaagtga ggtcatcata agggtggggc
     6781 cttaattcag tagaattggt ggcctcctaa gcagaggaag agagattttt ctttctctct
     6841 ctgccatgtg aagacagtga ggagtcggcc gtctgcaagc caagaagagc ccttatcagg
     6901 aacagacttg gctagcacct tcatcgtgga cctccagcct ccagaattgc aagaaaatac
     6961 atttccgtcg ttgaaaccac ccagtctgtg gtattttgtt atggcagccc aggcagacta
     7021 atacgtgaag cctgctctaa atagataaaa taagaaatta ctacagaggg ctctttagaa
     7081 attgtattta aaaacaagac aatccatatt tacctaagat ttacagaatg tatgtctata
     7141 aaaggaggga tttctggact agatgatgat gaaaaatgtt catataaagg cacctccagc
     7201 ttcgagttgc caacacagga ggaagaatgc tccctgctgt tcagatgctg atatgtgtcc
     7261 tgtgcttcct ggatggccag tgggatcata agctggtaga agcaagacct tcatccactg
     7321 acttcatatt tcttccacat cctgaactgt ggtgtttgac tttaaaaata aattaaagca
     7381 aatagaaatg tttccattga gattttggca aaaacccaca tgacatttgc gtctgcagag
     7441 tagacttgga aggcatgcac ctggaggcca agtggtattc tctggactct ctgtccttgc
     7501 atttgcgtgg ctgccccgag gactcgcttc ttcctggatg tgaagcagag gcacgatggg
     7561 gctgactgag aggctgaaat gtgcttctct tcacagttac atagttttct tctaattaaa
     7621 aataaatttt aagtggtaga ttatgaaaat tccatttgaa tcaagatttt aaaaattcaa
     7681 tatcaaacca gttgttactg tcttcactct ccgttgttta cgattgtgtg cttttcctga
     7741 ataaagaatg actggagaga ca
//
