LOCUS       NM_001387437            1584 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens amylase alpha 2B (AMY2B), transcript variant 3, mRNA.
ACCESSION   NM_001387437
VERSION     NM_001387437.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Date,K., Yamazaki,T., Toyoda,Y., Hoshi,K. and Ogawa,H.
  TITLE     alpha-Amylase expressed in human small intestinal epithelial cells
            is essential for cell proliferation and differentiation
  JOURNAL   J Cell Biochem 121 (2), 1238-1249 (2020)
   PUBMED   31478242
  REMARK    GeneRIF: alpha-Amylase expressed in human small intestinal
            epithelial cells is essential for cell proliferation and
            differentiation.
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Peng,Y.F., Goyal,H., Lin,H., Liu,D.C. and Li,L.
  TITLE     Serum amylase activity altered by the ABO blood group system in
            Chinese subjects
  JOURNAL   J Clin Lab Anal 33 (5), e22883 (2019)
   PUBMED   30938472
  REMARK    GeneRIF: A significant link between serum amylase activity and ABO
            blood type in Chinese subjects.
REFERENCE   3  (bases 1 to 1584)
  AUTHORS   Heianza,Y., Sun,D., Wang,T., Huang,T., Bray,G.A., Sacks,F.M. and
            Qi,L.
  TITLE     Starch Digestion-Related Amylase Genetic Variant Affects 2-Year
            Changes in Adiposity in Response to Weight-Loss Diets: The POUNDS
            Lost Trial
  JOURNAL   Diabetes 66 (9), 2416-2423 (2017)
   PUBMED   28659346
  REMARK    GeneRIF: It was concluded that the genetic variant determining
            starch metabolism influences the response to weight-loss dietary
            intervention. Overweight and obese individuals carrying the
            AMY1-AMY2 rs11185098 genotype associated with higher amylase
            activity may have greater loss of adiposity during low-calorie diet
            interventions.
REFERENCE   4  (bases 1 to 1584)
  AUTHORS   Omichi,K. and Hase,S.
  TITLE     Detection of human urinary alpha-amylase encoded by the AMY2B gene
            using a fluorogenic substrate, FG5P
  JOURNAL   J Biochem 112 (3), 303-305 (1992)
   PUBMED   1429515
REFERENCE   5  (bases 1 to 1584)
  AUTHORS   Tomita,N., Horii,A., Doi,S., Yokouchi,H., Shiosaki,K.,
            Higashiyama,M., Matsuura,N., Ogawa,M., Mori,T. and Matsubara,K.
  TITLE     A novel type of human alpha-amylase produced in lung carcinoid
            tumor
  JOURNAL   Gene 76 (1), 11-18 (1989)
   PUBMED   2701942
REFERENCE   6  (bases 1 to 1584)
  AUTHORS   Groot,P.C., Bleeker,M.J., Pronk,J.C., Arwert,F., Mager,W.H.,
            Planta,R.J., Eriksson,A.W. and Frants,R.R.
  TITLE     Human pancreatic amylase is encoded by two different genes
  JOURNAL   Nucleic Acids Res 16 (10), 4724 (1988)
   PUBMED   3260028
REFERENCE   7  (bases 1 to 1584)
  AUTHORS   Gumucio,D.L., Wiebauer,K., Caldwell,R.M., Samuelson,L.C. and
            Meisler,M.H.
  TITLE     Concerted evolution of human amylase genes
  JOURNAL   Mol Cell Biol 8 (3), 1197-1205 (1988)
   PUBMED   2452973
REFERENCE   8  (bases 1 to 1584)
  AUTHORS   Tricoli,J.V. and Shows,T.B.
  TITLE     Regional assignment of human amylase (AMY) to p22----p21 of
            chromosome 1
  JOURNAL   Somat Cell Mol Genet 10 (2), 205-210 (1984)
   PUBMED   6608795
REFERENCE   9  (bases 1 to 1584)
  AUTHORS   Rosenblum,B.B. and Merritt,A.D.
  TITLE     Human pancreatic alpha-amylase: phenotypic codominance and new
            electrophoretic variants
  JOURNAL   Am J Hum Genet 30 (4), 434-441 (1978)
   PUBMED   309725
REFERENCE   10 (bases 1 to 1584)
  AUTHORS   Kaczmarek,M.J. and Rosenmund,H.
  TITLE     The action of human pancreatic and salivary isoamylases on starch
            and glycogen
  JOURNAL   Clin Chim Acta 79 (1), 69-73 (1977)
   PUBMED   890964
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC105272.2.
            
            Summary: Amylases are secreted proteins that hydrolyze
            1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides,
            and thus catalyze the first step in digestion of dietary starch and
            glycogen. The human genome has a cluster of several amylase genes
            that are expressed at high levels in either salivary gland or
            pancreas. This gene encodes an amylase isoenzyme produced by the
            pancreas. [provided by RefSeq, Jun 2013].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M24895.1, DRR138519.536507.1
                                           [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000684275.1/ ENSP00000507176.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-182               AC105272.2         21896-22077
            183-329             AC105272.2         22417-22563
            330-527             AC105272.2         23370-23567
            528-758             AC105272.2         24015-24245
            759-892             AC105272.2         24567-24700
            893-1015            AC105272.2         25530-25652
            1016-1115           AC105272.2         25748-25847
            1116-1234           AC105272.2         27797-27915
            1235-1360           AC105272.2         28027-28152
            1361-1584           AC105272.2         29618-29841
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p21.1"
     gene            1..1584
                     /gene="AMY2B"
                     /gene_synonym="AMY2; AMY3; HXA"
                     /note="amylase alpha 2B"
                     /db_xref="GeneID:280"
                     /db_xref="HGNC:HGNC:478"
                     /db_xref="MIM:104660"
     exon            1..182
                     /gene="AMY2B"
                     /gene_synonym="AMY2; AMY3; HXA"
                     /inference="alignment:Splign:2.1.0"
     CDS             15..1550
                     /gene="AMY2B"
                     /gene_synonym="AMY2; AMY3; HXA"
                     /EC_number="3.2.1.1"
                     /note="glycogenase; alpha-amylase 2B; carcinoid
                     alpha-amylase; 1,4-alpha-D-glucan glucanohydrolase 2B;
                     amylase alpha 2B (pancreatic)"
                     /codon_start=1
                     /product="alpha-amylase 2B precursor"
                     /protein_id="NP_001374366.1"
                     /db_xref="CCDS:CCDS782.1"
                     /db_xref="GeneID:280"
                     /db_xref="HGNC:HGNC:478"
                     /db_xref="MIM:104660"
                     /translation="MKFFLLLFTIGFCWAQYSPNTQQGRTSIVHLFEWRWVDIALECE
                     RYLAPKGFGGVQVSPPNENVAIHNPFRPWWERYQPVSYKLCTRSGNEDEFRNMVTRCN
                     NVGVRIYVDAVINHMSGNAVSAGTSSTCGSYFNPGSRDFPAVPYSGWDFNDGKCKTGS
                     GDIENYNDATQVRDCRLVGLLDLALEKDYVRSKIAEYMNHLIDIGVAGFRLDASKHMW
                     PGDIKAILDKLHNLNSNWFPAGSKPFIYQEVIDLGGEPIKSSDYFGNGRVTEFKYGAK
                     LGTVIRKWNGEKMSYLKNWGEGWGFMPSDRALVFVDNHDNQRGHGAGGASILTFWDAR
                     LYKMAVGFMLAHPYGFTRVMSSYRWPRQFQNGNDVNDWVGPPNNNGVIKEVTINPDTT
                     CGNDWVCEHRWRQIRNMVNFRNVVDGQPFTNWYDNGSNQVAFGRGNRGFIVFNNDDWT
                     FSLTLQTGLPAGTYCDVISGDKINGNCTGIKIYVSDDGKAHFSISNSAEDPFIAIHAE
                     SKL"
     exon            183..329
                     /gene="AMY2B"
                     /gene_synonym="AMY2; AMY3; HXA"
                     /inference="alignment:Splign:2.1.0"
     exon            330..527
                     /gene="AMY2B"
                     /gene_synonym="AMY2; AMY3; HXA"
                     /inference="alignment:Splign:2.1.0"
     exon            528..758
                     /gene="AMY2B"
                     /gene_synonym="AMY2; AMY3; HXA"
                     /inference="alignment:Splign:2.1.0"
     exon            759..892
                     /gene="AMY2B"
                     /gene_synonym="AMY2; AMY3; HXA"
                     /inference="alignment:Splign:2.1.0"
     exon            893..1015
                     /gene="AMY2B"
                     /gene_synonym="AMY2; AMY3; HXA"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1115
                     /gene="AMY2B"
                     /gene_synonym="AMY2; AMY3; HXA"
                     /inference="alignment:Splign:2.1.0"
     exon            1116..1234
                     /gene="AMY2B"
                     /gene_synonym="AMY2; AMY3; HXA"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..1360
                     /gene="AMY2B"
                     /gene_synonym="AMY2; AMY3; HXA"
                     /inference="alignment:Splign:2.1.0"
     exon            1361..1584
                     /gene="AMY2B"
                     /gene_synonym="AMY2; AMY3; HXA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1559..1564
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AMY2B"
                     /gene_synonym="AMY2; AMY3; HXA"
                     /note="hexamer: ATTAAA"
     polyA_site      1584
                     /gene="AMY2B"
                     /gene_synonym="AMY2; AMY3; HXA"
                     /note="major polyA site"
ORIGIN      
        1 aacttcaaag caaaatgaag ttctttctgt tgcttttcac cattgggttc tgctgggctc
       61 agtattcccc aaatacacaa caaggacgga catctattgt tcatctgttt gaatggcgat
      121 gggttgatat tgctcttgaa tgtgagcgat atttagctcc caagggattt ggaggggttc
      181 aggtctctcc accaaatgaa aatgttgcaa ttcacaaccc tttcagacct tggtgggaaa
      241 gataccaacc agttagctat aaattatgca caagatctgg aaatgaagat gaatttagaa
      301 acatggtgac tagatgtaac aatgttgggg ttcgtattta tgtggatgct gtaattaatc
      361 atatgtctgg taatgctgtg agtgcaggaa caagcagtac ctgtggaagt tacttcaacc
      421 ctggaagtag ggactttcca gcagtcccat attctggatg ggattttaat gatggtaaat
      481 gtaaaactgg aagtggagat atcgagaact acaatgatgc tactcaggtc agagattgtc
      541 gtctggttgg tcttcttgat cttgcactgg agaaagatta tgtgcgttcc aagattgccg
      601 aatatatgaa tcatctcatt gacattggtg ttgcagggtt cagacttgat gcttccaagc
      661 acatgtggcc tggagacata aaggcaattt tggacaaact gcataatcta aacagtaact
      721 ggttccctgc aggaagtaaa cctttcattt accaggaggt aattgatctg ggtggtgagc
      781 caattaaaag cagtgactac tttggaaatg gccgggtgac agaattcaag tatggtgcaa
      841 aactcggcac agttattcgc aagtggaatg gagagaagat gtcttaccta aagaactggg
      901 gagaaggttg gggtttcatg ccttctgaca gagcacttgt ctttgtggat aaccatgaca
      961 atcaacgagg acatggggct ggaggagcct ctattcttac cttctgggat gctaggctgt
     1021 ataaaatggc agttggattt atgcttgctc atccttatgg ttttacacga gtaatgtcaa
     1081 gctaccgttg gccaagacag tttcaaaatg gaaacgatgt taatgattgg gttgggccac
     1141 caaataataa tggagtaatt aaagaagtta ctattaatcc agacactact tgtggcaatg
     1201 actgggtctg tgaacatcga tggcgccaaa taaggaacat ggttaatttc cgcaatgtag
     1261 tggatggcca gccttttaca aactggtatg ataatgggag caaccaagtg gcttttggga
     1321 gaggaaacag aggattcatt gttttcaaca atgatgactg gacattttct ttaactttgc
     1381 aaactggtct tcctgctggc acatactgtg atgtcatttc tggagataaa attaatggca
     1441 attgcacagg cattaaaatc tacgtttctg acgatggcaa agctcatttt tctattagta
     1501 actctgctga ggatccattt attgcaattc atgctgaatc taaattataa aatttaaaat
     1561 taaatgcata tcctcaaaac aata
//
