LOCUS       NM_015365               5350 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens AMMECR nuclear protein 1 (AMMECR1), transcript variant
            1, mRNA.
ACCESSION   NM_015365
VERSION     NM_015365.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 5350)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5350)
  AUTHORS   Ge,H., Cheng,N., Xu,X., Yang,Z., Hoffman,R.M. and Zhu,J.
  TITLE     AMMECR1 Inhibits Apoptosis and Promotes Cell-cycle Progression and
            Proliferation of the A549 Human Lung Cancer Cell Line
  JOURNAL   Anticancer Res 39 (9), 4637-4642 (2019)
   PUBMED   31519561
  REMARK    GeneRIF: AMMECR1 plays a critical role in cell proliferation,
            cell-cycle progression, and apoptosis of human lung cancer cells,
            and may serve as a potential therapeutic target for non-small-cell
            lung cancer.
REFERENCE   5  (bases 1 to 5350)
  AUTHORS   Moyses-Oliveira,M., Giannuzzi,G., Fish,R.J., Rosenfeld,J.A.,
            Petit,F., Soares,M.F., Kulikowski,L.D., Di-Battista,A.,
            Zamariolli,M., Xia,F., Liehr,T., Kosyakova,N., Carvalheira,G.,
            Parker,M., Seaby,E.G., Ennis,S., Gilbert,R.D., Hagelstrom,R.T.,
            Cremona,M.L., Li,W.L., Malhotra,A., Chandrasekhar,A., Perry,D.L.,
            Taft,R.J., McCarrier,J., Basel,D.G., Andrieux,J., Stumpp,T.,
            Antunes,F., Pereira,G.J., Neerman-Arbez,M., Meloni,V.A.,
            Drummond-Borg,M., Melaragno,M.I. and Reymond,A.
  TITLE     Inactivation of AMMECR1 is associated with growth, bone, and heart
            alterations
  JOURNAL   Hum Mutat 39 (2), 281-291 (2018)
   PUBMED   29193635
  REMARK    GeneRIF: results suggest that AMMECR1 is potentially involved in
            cell cycle control and linked to a new syndrome with growth, bone,
            heart, and kidney alterations with or without elliptocytosis.
REFERENCE   6  (bases 1 to 5350)
  AUTHORS   Tanneberger,K., Pfister,A.S., Brauburger,K., Schneikert,J.,
            Hadjihannas,M.V., Kriz,V., Schulte,G., Bryja,V. and Behrens,J.
  TITLE     Amer1/WTX couples Wnt-induced formation of PtdIns(4,5)P2 to LRP6
            phosphorylation
  JOURNAL   EMBO J 30 (8), 1433-1443 (2011)
   PUBMED   21304492
REFERENCE   7  (bases 1 to 5350)
  AUTHORS   Ross,M.T., Grafham,D.V., Coffey,A.J., Scherer,S., McLay,K.,
            Muzny,D., Platzer,M., Howell,G.R., Burrows,C., Bird,C.P.,
            Frankish,A., Lovell,F.L., Howe,K.L., Ashurst,J.L., Fulton,R.S.,
            Sudbrak,R., Wen,G., Jones,M.C., Hurles,M.E., Andrews,T.D.,
            Scott,C.E., Searle,S., Ramser,J., Whittaker,A., Deadman,R.,
            Carter,N.P., Hunt,S.E., Chen,R., Cree,A., Gunaratne,P., Havlak,P.,
            Hodgson,A., Metzker,M.L., Richards,S., Scott,G., Steffen,D.,
            Sodergren,E., Wheeler,D.A., Worley,K.C., Ainscough,R.,
            Ambrose,K.D., Ansari-Lari,M.A., Aradhya,S., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Ballabio,A., Banerjee,R., Barker,G.E.,
            Barlow,K.F., Barrett,I.P., Bates,K.N., Beare,D.M., Beasley,H.,
            Beasley,O., Beck,A., Bethel,G., Blechschmidt,K., Brady,N.,
            Bray-Allen,S., Bridgeman,A.M., Brown,A.J., Brown,M.J., Bonnin,D.,
            Bruford,E.A., Buhay,C., Burch,P., Burford,D., Burgess,J.,
            Burrill,W., Burton,J., Bye,J.M., Carder,C., Carrel,L., Chako,J.,
            Chapman,J.C., Chavez,D., Chen,E., Chen,G., Chen,Y., Chen,Z.,
            Chinault,C., Ciccodicola,A., Clark,S.Y., Clarke,G., Clee,C.M.,
            Clegg,S., Clerc-Blankenburg,K., Clifford,K., Cobley,V., Cole,C.G.,
            Conquer,J.S., Corby,N., Connor,R.E., David,R., Davies,J., Davis,C.,
            Davis,J., Delgado,O., Deshazo,D., Dhami,P., Ding,Y., Dinh,H.,
            Dodsworth,S., Draper,H., Dugan-Rocha,S., Dunham,A., Dunn,M.,
            Durbin,K.J., Dutta,I., Eades,T., Ellwood,M., Emery-Cohen,A.,
            Errington,H., Evans,K.L., Faulkner,L., Francis,F., Frankland,J.,
            Fraser,A.E., Galgoczy,P., Gilbert,J., Gill,R., Glockner,G.,
            Gregory,S.G., Gribble,S., Griffiths,C., Grocock,R., Gu,Y.,
            Gwilliam,R., Hamilton,C., Hart,E.A., Hawes,A., Heath,P.D.,
            Heitmann,K., Hennig,S., Hernandez,J., Hinzmann,B., Ho,S., Hoffs,M.,
            Howden,P.J., Huckle,E.J., Hume,J., Hunt,P.J., Hunt,A.R.,
            Isherwood,J., Jacob,L., Johnson,D., Jones,S., de Jong,P.J.,
            Joseph,S.S., Keenan,S., Kelly,S., Kershaw,J.K., Khan,Z.,
            Kioschis,P., Klages,S., Knights,A.J., Kosiura,A., Kovar-Smith,C.,
            Laird,G.K., Langford,C., Lawlor,S., Leversha,M., Lewis,L., Liu,W.,
            Lloyd,C., Lloyd,D.M., Loulseged,H., Loveland,J.E., Lovell,J.D.,
            Lozado,R., Lu,J., Lyne,R., Ma,J., Maheshwari,M., Matthews,L.H.,
            McDowall,J., McLaren,S., McMurray,A., Meidl,P., Meitinger,T.,
            Milne,S., Miner,G., Mistry,S.L., Morgan,M., Morris,S., Muller,I.,
            Mullikin,J.C., Nguyen,N., Nordsiek,G., Nyakatura,G., O'Dell,C.N.,
            Okwuonu,G., Palmer,S., Pandian,R., Parker,D., Parrish,J.,
            Pasternak,S., Patel,D., Pearce,A.V., Pearson,D.M., Pelan,S.E.,
            Perez,L., Porter,K.M., Ramsey,Y., Reichwald,K., Rhodes,S.,
            Ridler,K.A., Schlessinger,D., Schueler,M.G., Sehra,H.K.,
            Shaw-Smith,C., Shen,H., Sheridan,E.M., Shownkeen,R., Skuce,C.D.,
            Smith,M.L., Sotheran,E.C., Steingruber,H.E., Steward,C.A.,
            Storey,R., Swann,R.M., Swarbreck,D., Tabor,P.E., Taudien,S.,
            Taylor,T., Teague,B., Thomas,K., Thorpe,A., Timms,K., Tracey,A.,
            Trevanion,S., Tromans,A.C., d'Urso,M., Verduzco,D., Villasana,D.,
            Waldron,L., Wall,M., Wang,Q., Warren,J., Warry,G.L., Wei,X.,
            West,A., Whitehead,S.L., Whiteley,M.N., Wilkinson,J.E.,
            Willey,D.L., Williams,G., Williams,L., Williamson,A.,
            Williamson,H., Wilming,L., Woodmansey,R.L., Wray,P.W., Yen,J.,
            Zhang,J., Zhou,J., Zoghbi,H., Zorilla,S., Buck,D., Reinhardt,R.,
            Poustka,A., Rosenthal,A., Lehrach,H., Meindl,A., Minx,P.J.,
            Hillier,L.W., Willard,H.F., Wilson,R.K., Waterston,R.H., Rice,C.M.,
            Vaudin,M., Coulson,A., Nelson,D.L., Weinstock,G., Sulston,J.E.,
            Durbin,R., Hubbard,T., Gibbs,R.A., Beck,S., Rogers,J. and
            Bentley,D.R.
  TITLE     The DNA sequence of the human X chromosome
  JOURNAL   Nature 434 (7031), 325-337 (2005)
   PUBMED   15772651
REFERENCE   8  (bases 1 to 5350)
  AUTHORS   Tajika,Y., Sakai,N., Tamura,T., Yao,M., Watanabe,N. and Tanaka,I.
  TITLE     Crystal structure of PH0010 from Pyrococcus horikoshii, which is
            highly homologous to human AMMECR 1C-terminal region
  JOURNAL   Proteins 58 (2), 501-503 (2005)
   PUBMED   15558565
  REMARK    GeneRIF: PH0010 from Pyrococcus horikoshii is highly homologous to
            human AMMECR 1C-terminal region
REFERENCE   9  (bases 1 to 5350)
  AUTHORS   Vitelli,F., Piccini,M., Caroli,F., Franco,B., Malandrini,A.,
            Pober,B., Jonsson,J., Sorrentino,V. and Renieri,A.
  TITLE     Identification and characterization of a highly conserved protein
            absent in the Alport syndrome (A), mental retardation (M), midface
            hypoplasia (M), and elliptocytosis (E) contiguous gene deletion
            syndrome (AMME)
  JOURNAL   Genomics 55 (3), 335-340 (1999)
   PUBMED   10049589
REFERENCE   10 (bases 1 to 5350)
  AUTHORS   Piccini,M., Vitelli,F., Bruttini,M., Pober,B.R., Jonsson,J.J.,
            Villanova,M., Zollo,M., Borsani,G., Ballabio,A. and Renieri,A.
  TITLE     FACL4, a new gene encoding long-chain acyl-CoA synthetase 4, is
            deleted in a family with Alport syndrome, elliptocytosis, and
            mental retardation
  JOURNAL   Genomics 47 (3), 350-358 (1998)
   PUBMED   9480748
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AJ007014.1, BC060813.1,
            BC037983.1 and BC051895.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_015365.2.
            
            Summary: The exact function of this gene is not known, however,
            submicroscopic deletion of the X chromosome including this gene,
            COL4A5, and FACL4 genes, result in a contiguous gene deletion
            syndrome, the AMME complex (Alport syndrome, mental retardation,
            midface hypoplasia, and elliptocytosis). Alternatively spliced
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Jan 2010].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.76746.1,
                                           SRR1660809.67929.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262844.10/ ENSP00000262844.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1146              AJ007014.1         155-1300
            1147-3000           BC060813.1         1036-2889
            3001-5229           BC037983.1         33-2261
            5230-5350           BC051895.1         2197-2317
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq23"
     gene            1..5350
                     /gene="AMMECR1"
                     /gene_synonym="AMMERC1; MFHIEN"
                     /note="AMMECR nuclear protein 1"
                     /db_xref="GeneID:9949"
                     /db_xref="HGNC:HGNC:467"
                     /db_xref="MIM:300195"
     exon            1..487
                     /gene="AMMECR1"
                     /gene_synonym="AMMERC1; MFHIEN"
                     /inference="alignment:Splign:2.1.0"
     CDS             15..1016
                     /gene="AMMECR1"
                     /gene_synonym="AMMERC1; MFHIEN"
                     /note="isoform 1 is encoded by transcript variant 1; AMME
                     syndrome candidate gene 1 protein; nuclear protein
                     AMMECR1"
                     /codon_start=1
                     /product="nuclear protein AMMECR1 isoform 1"
                     /protein_id="NP_056180.1"
                     /db_xref="CCDS:CCDS14551.1"
                     /db_xref="GeneID:9949"
                     /db_xref="HGNC:HGNC:467"
                     /db_xref="MIM:300195"
                     /translation="MAAGCCGVKKQKLSSSPPSGSGGGGGASSSSHCSGESQCRAGEL
                     GLGGAGTRLNGLGGLTGGGSGSGCTLSPPQGCGGGGGGIALSPPPSCGVGTLLSTPAA
                     ATSSSPSSSSAASSSSPGSRKMVVSAEMCCFCFDVLYCHLYGYQQPRTPRFTNEPYPL
                     FVTWKIGRDKRLRGCIGTFSAMNLHSGLREYTLTSALKDSRFPPMTRDELPRLFCSVS
                     LLTNFEDVCDYLDWEVGVHGIRIEFINEKGSKRTATYLPEVAKEQGWDHIQTIDSLLR
                     KGGYKAPITNEFRKTIKLTRYRSEKMTLSYAEYLAHRQHHHFQNGIGHPLPPYNHYS"
     exon            488..598
                     /gene="AMMECR1"
                     /gene_synonym="AMMERC1; MFHIEN"
                     /inference="alignment:Splign:2.1.0"
     exon            599..713
                     /gene="AMMECR1"
                     /gene_synonym="AMMERC1; MFHIEN"
                     /inference="alignment:Splign:2.1.0"
     exon            714..804
                     /gene="AMMECR1"
                     /gene_synonym="AMMERC1; MFHIEN"
                     /inference="alignment:Splign:2.1.0"
     exon            805..901
                     /gene="AMMECR1"
                     /gene_synonym="AMMERC1; MFHIEN"
                     /inference="alignment:Splign:2.1.0"
     exon            902..5350
                     /gene="AMMECR1"
                     /gene_synonym="AMMERC1; MFHIEN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5332..5337
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AMMECR1"
                     /gene_synonym="AMMERC1; MFHIEN"
                     /note="hexamer: AATAAA"
     polyA_site      5350
                     /gene="AMMECR1"
                     /gene_synonym="AMMERC1; MFHIEN"
                     /note="major polyA site"
ORIGIN      
        1 gagactgttc caagatggcg gcgggttgct gcggggtgaa gaagcagaaa ctgtccagtt
       61 cgcccccctc tggctcgggt ggcggtggtg gcgcctcctc ctcctcccac tgcagcggag
      121 agagccagtg ccgagctggg gagctgggac taggaggcgc cggtacgcgg ctcaacgggc
      181 tgggaggtct aaccggagga ggtagcggca gcggctgtac cctctctccc ccccagggct
      241 gcggcggcgg cggcgggggg atcgccctgt cgccacctcc gagctgcgga gtggggaccc
      301 tactttctac cccggccgcc gccacctctt cctcaccctc ctcatcgtcc gccgcctcgt
      361 cctcatcgcc gggctcccgg aagatggtgg tgtcagcaga gatgtgctgc ttttgcttcg
      421 atgtgctcta ctgtcacctg tatggatacc agcagccccg gaccccccga ttcaccaacg
      481 agccctaccc actgtttgta acatggaaga ttggtcgaga caaaagatta cgtggatgca
      541 taggtacttt ttctgccatg aatttgcatt caggactcag ggagtacaca cttaccagtg
      601 cccttaaaga tagccgtttt cccccaatga caagggatga gctgccacgg cttttctgct
      661 cagtgtctct gctcactaac tttgaagatg tctgtgatta tttggactgg gaggtgggtg
      721 tacatggcat tagaatagaa ttcatcaatg aaaaaggatc aaaacgcacc gccacctacc
      781 taccggaggt tgcaaaggag caaggatggg accatataca gaccatagac tccttattga
      841 ggaaaggagg atacaaagct ccgattacta atgaattcag gaaaaccata aaactgacca
      901 ggtatcgtag tgaaaagatg accctgagct atgctgaata ccttgctcat cgccagcatc
      961 atcatttcca aaatggcatt gggcatcccc ttccgccata caaccattat tcctgacact
     1021 gagccgcaca accagtcact gggcctctct gcagacctct tcccaggaga ccctacacct
     1081 tcttggtcta gctatctctt ttactgtacc attttatgat gatagtttcc gttgccatgg
     1141 tgaagcttcg acatcgtcaa ctaagatcat catggtaacg ggtagaaaaa tggcatttgt
     1201 taagatcctg ttttattatt ttagatcgtt gatttttttt ttgcagcata tataattttg
     1261 ggtttttttt tccttataat attatgtaga attttgcttt gctgtctcag tcaggtttct
     1321 gtttttctgt cctccttgcc tttcttcccg ttttgcttcc tgttttcctt cttccttccc
     1381 gttttccttc ttccttccct gcctcccttc ctttgactgt ggatggaaga aagtgtgcag
     1441 tttttaggga ttttacttag gttcgtcttt agttttcctc agtaagatag ttgttttttg
     1501 atacctgagt ttgggattaa ttcatatcaa attcaggtat ttgtatatta ctcttgattt
     1561 ttgtctgaaa ttcactttgc tatgacagcc tagtagttgg gtcttcactc cttaagtata
     1621 tgtttttccc atggtgaaaa tacatgaact ttagttctag tagtgagcat tcaaaggtcc
     1681 gtgatagggc ttgtcacaga gagagagaaa tcatttatcc ctattgtgct ggttatcata
     1741 gaagagactg cctcaccact ttatagaacc aatttgcaaa ctaaaacact gtttggagaa
     1801 ggtacaaact acctttcaaa aaggctacag gaaacatttt atgagcagca aatattagga
     1861 agagagcaga tacagaattt agtgcctttg agaagaatat aattaagtgg aattaagagg
     1921 ggttagaaaa aaggcaattt agaaaaatat tcttgtaaaa ctttattgtt tctatatgtg
     1981 aaacagaaga ttagacaaat tccttactct taagtatttt tttaagctga aaaaaaatta
     2041 ttttgagcaa ataaccaata agagacattt gtctatttta tattaattta aatttgttag
     2101 ctctaggttt caataagtca tgattggcta gaatccattt tagctaccat ctaaagatat
     2161 ggtaaagata atttagtaaa acaaaagttc atatttgcca tgatttcaga tatgtttgtc
     2221 atggggacaa tattagagtt ttagagatac tataaaatag cttgaatttc tttctgcact
     2281 acatactttt gatagaaaca catttactac attgataaga tgttatgtca cacctgtgaa
     2341 aatggagttg gcattcagat atgctatagt tagagcccag ctcatctgca agtatagtct
     2401 tcccatgatg catacaaaga taacttactg atgtcgatag gaggtccata catatatccc
     2461 caggaaaaat gacagattat tctggaggaa gatgaccttc attttatggt tatgtgtgtg
     2521 tgtctgtgtg tctttggaaa aatatatata attttttcaa ataggaagcc aacatatcaa
     2581 gtgatgaatt aaagtatgct gcagaatata tattctaaaa ctacaaaaaa gtcactgaat
     2641 atcaaaatga tacagcttat acatatagtt actgtgacaa gtgacagact gctagttcag
     2701 aattcaaaaa tcctttccta gtttgtgaga taatgggcta aattccttct gcctgccact
     2761 ggggcaaagc aaattgcttt agtttttgat gagagttctt agaagtttgt tggtattcct
     2821 tcatccacag catccattgt tgaaataacc attttcagtt gtgatgcctt aactaagaag
     2881 ccaattgtta gcctgaaatg caatcttggt agccagtttc aatgaagcta gagattagtc
     2941 agaaaaagtt agctgttggg ctttagaaag ggattttgag tcctgtcatt tctacttggg
     3001 agcattttgg agcagattag tctttcagta taaaaacaag tggctacctg atggaaactt
     3061 ttcttaccct tatagggaaa ctgagcacaa gctgaatgat attgtctgct gcaaaaaaaa
     3121 acaaacaaaa aaaaaacaaa acaaaaaaca aaaaaaagga aaaaaaggta aataaaacaa
     3181 gcaaactgaa gagagacaaa atagaccaat attatcgtga aatccatctt atccatcatc
     3241 tcatcggttc aacaggctcc tcgtcctggg tgagcttatg gttagaagtt tttttagcaa
     3301 agatttatta catacataga tgtgtgtgtg tacatatact tacacataca tacatacata
     3361 tatgtgtgtt tatttaacgg tgctaatcaa tcttgagcag gtcacgtgac tggtcatgcg
     3421 gactctaaaa tcatatcaga ttttttaaaa tccttgatat atgcagatgt tatacagatt
     3481 ttcttagcag tgtaataacg ttaaactgaa gaaataacca tcctgcaggc ttcaaaggac
     3541 gaggtcagca atatttccca acatggcttg ggggaaaagg atagttttgt ctccttttgt
     3601 attcaagtaa tgcaaagtgc atttttgtct ctaagtagct tttgactaaa agactttgta
     3661 gtgtaaaggg tgttatacaa actgtaatgg tgtgcttcaa acaaatgcta gaccctttca
     3721 cctttgtcgt atatacaaag ctgttaatta tgtggtatga attaactttg aacatgttga
     3781 aatatgtagc atgtctttaa ctcagacgaa gattctgatt gcacaggttg tgttctagcc
     3841 agttgtgatt tatttgttca gaaacacaaa tgtgaattgc actcttatca tcagaatatt
     3901 gtatgagttc agtgatcttt aaacagctca gaagtaatac ttgaacttca tttgagtagc
     3961 acaaagttta catagcccct tttaaatgtt aattcgttcc atttaagttt tccattttca
     4021 gatgcaactg ggtatttttc ctcttggaaa tagtacgtac tttgctttgg tatgtagcaa
     4081 aaggcttcca tttctggaag ggttttcttg ttcttaaaca aggtcagcca ggtaccagat
     4141 tctaaaagaa caaattgtcc cccacccctc aagtggcctt ctgactgcac gagaatgtgc
     4201 tactaccctc tgtagtctcc cctaacacga gaggtcagtg aaattgatgg aggaagtgca
     4261 aggcttgctt cccctcaccc ttaaagctta aagcagttgt aagattttca gtccagcaca
     4321 tacagtccca gtgcccagca caccggaagt ttgtatactg gcctcttatc ttgtcttcaa
     4381 atgtgcagga aatttgaaac tgtcatctgg aataggttcc atctcttatg tgtgttagat
     4441 caaaccaaag aagcccccag ccatgcccaa atgctgcttc aaaagcctgc cttccagtcc
     4501 ttacaaaaac cttgcaggat taaatgtgtt aactttttta tttttgtaca gtttctaagt
     4561 gatttttgct agtgatgtta aaatgaatta agtttatttg aggggtgtga gcacctcctc
     4621 catttaaata aactggtgac tttcctttta ttttttaaaa gtggaaaccc gttgtgtgcc
     4681 tctcgattta agggtttctg atgacattat tcttaagacc agcattgatc ctttatatac
     4741 aaatatatgt tttccttgtt ttttattgct atttcagaag aaaatgaacc ttttattttg
     4801 ctctacccag taactgcgct ggtacataga cgcactgagc aaacaaactt ttgtaaccac
     4861 ctctgactac ttttgtatat caaagttgat tacttttgaa atatttggat ctagtttcta
     4921 agttatttta gtgttttata tgttccccaa aattactttg aatctgtcac cagcataatg
     4981 agttcttggt gatacaatgg tgagacttgt aatgagcaaa aagctacctg gagtagctcc
     5041 tcttttcctt ctttcttagt aacctgtttt ttgattaccg taagacatca cagattacct
     5101 gaaacccctt ccagtataac taggccctcc ttccttccac atttaattgc ttaatagttt
     5161 gaagaaacta ttggaaatgg gcattttata tgacatgtat ggctttacaa tattaaatat
     5221 gagcaaaaag aaaaaatatg agaaggttta tgtgggtctg taaggtatgg ctgtggaaag
     5281 atattgtagt agttttgaca ctattgttat tacctttaaa atcatttacc caataaaatg
     5341 ttaaaaccga
//
