LOCUS       NM_152424               8407 bp    mRNA    linear   PRI 06-OCT-2024
DEFINITION  Homo sapiens APC membrane recruitment protein 1 (AMER1), mRNA.
ACCESSION   NM_152424
VERSION     NM_152424.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Lei,S., Chen,C., Han,F., Deng,J., Huang,D., Qian,L., Zhu,M., Ma,X.,
            Lai,M., Xu,E. and Zhang,H.
  TITLE     AMER1 deficiency promotes the distant metastasis of colorectal
            cancer by inhibiting SLC7A11- and FTL-mediated ferroptosis
  JOURNAL   Cell Rep 42 (9), 113110 (2023)
   PUBMED   37682704
  REMARK    GeneRIF: AMER1 deficiency promotes the distant metastasis of
            colorectal cancer by inhibiting SLC7A11- and FTL-mediated
            ferroptosis.
REFERENCE   2  (bases 1 to 8407)
  AUTHORS   Chen,Y.J., Luo,S.N., Wu,H., Zhang,N.P., Dong,L., Liu,T.T., Liang,L.
            and Shen,X.Z.
  TITLE     IRF-2 inhibits cancer proliferation by promoting AMER-1
            transcription in human gastric cancer
  JOURNAL   J Transl Med 20 (1), 68 (2022)
   PUBMED   35115027
  REMARK    GeneRIF: IRF-2 inhibits cancer proliferation by promoting AMER-1
            transcription in human gastric cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8407)
  AUTHORS   Wegert,J., Wittmann,S., Leuschner,I., Geissinger,E., Graf,N. and
            Gessler,M.
  TITLE     WTX inactivation is a frequent, but late event in Wilms tumors
            without apparent clinical impact
  JOURNAL   Genes Chromosomes Cancer 48 (12), 1102-1111 (2009)
   PUBMED   19760609
  REMARK    GeneRIF: WTX inactivation is associated with Wilms tumors
REFERENCE   4  (bases 1 to 8407)
  AUTHORS   Rivera,M.N., Kim,W.J., Wells,J., Stone,A., Burger,A., Coffman,E.J.,
            Zhang,J. and Haber,D.A.
  TITLE     The tumor suppressor WTX shuttles to the nucleus and modulates WT1
            activity
  JOURNAL   Proc Natl Acad Sci U S A 106 (20), 8338-8343 (2009)
   PUBMED   19416806
  REMARK    GeneRIF: WTX binds WT1 and enhances WT1-mediated transcription,
            suggesting a role for WTX in nuclear pathways implicated in the
            transcriptional regulation of cellular differentiation programs
REFERENCE   5  (bases 1 to 8407)
  AUTHORS   Yoo,N.J., Kim,S. and Lee,S.H.
  TITLE     Mutational analysis of WTX gene in Wnt/ beta-catenin pathway in
            gastric, colorectal, and hepatocellular carcinomas
  JOURNAL   Dig Dis Sci 54 (5), 1011-1014 (2009)
   PUBMED   18720004
  REMARK    GeneRIF: Data indicate that WTX mutation is rare in colorectal,
            gastric, and hepatocellular carcinomas.
REFERENCE   6  (bases 1 to 8407)
  AUTHORS   Major,M.B., Camp,N.D., Berndt,J.D., Yi,X., Goldenberg,S.J.,
            Hubbert,C., Biechele,T.L., Gingras,A.C., Zheng,N., Maccoss,M.J.,
            Angers,S. and Moon,R.T.
  TITLE     Wilms tumor suppressor WTX negatively regulates WNT/beta-catenin
            signaling
  JOURNAL   Science 316 (5827), 1043-1046 (2007)
   PUBMED   17510365
  REMARK    GeneRIF: findings show that WTX, a protein encoded by a gene
            mutated in Wilms tumors, forms a complex with beta-catenin, AXIN1,
            beta-TrCP2 and APC; data provide a possible mechanistic explanation
            for the tumor suppressor activity of WTX
REFERENCE   7  (bases 1 to 8407)
  AUTHORS   Rivera,M.N., Kim,W.J., Wells,J., Driscoll,D.R., Brannigan,B.W.,
            Han,M., Kim,J.C., Feinberg,A.P., Gerald,W.L., Vargas,S.O., Chin,L.,
            Iafrate,A.J., Bell,D.W. and Haber,D.A.
  TITLE     An X chromosome gene, WTX, is commonly inactivated in Wilms tumor
  JOURNAL   Science 315 (5812), 642-645 (2007)
   PUBMED   17204608
  REMARK    GeneRIF: WTX, is inactivated in approximately one-third of Wilms
            tumors; it is inactivated by a monoallelic 'single-hit' event
            targeting the single X chromosome in tumors from males and the
            active X chromosome in tumors from females
REFERENCE   8  (bases 1 to 8407)
  AUTHORS   Ross,M.T., Grafham,D.V., Coffey,A.J., Scherer,S., McLay,K.,
            Muzny,D., Platzer,M., Howell,G.R., Burrows,C., Bird,C.P.,
            Frankish,A., Lovell,F.L., Howe,K.L., Ashurst,J.L., Fulton,R.S.,
            Sudbrak,R., Wen,G., Jones,M.C., Hurles,M.E., Andrews,T.D.,
            Scott,C.E., Searle,S., Ramser,J., Whittaker,A., Deadman,R.,
            Carter,N.P., Hunt,S.E., Chen,R., Cree,A., Gunaratne,P., Havlak,P.,
            Hodgson,A., Metzker,M.L., Richards,S., Scott,G., Steffen,D.,
            Sodergren,E., Wheeler,D.A., Worley,K.C., Ainscough,R.,
            Ambrose,K.D., Ansari-Lari,M.A., Aradhya,S., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Ballabio,A., Banerjee,R., Barker,G.E.,
            Barlow,K.F., Barrett,I.P., Bates,K.N., Beare,D.M., Beasley,H.,
            Beasley,O., Beck,A., Bethel,G., Blechschmidt,K., Brady,N.,
            Bray-Allen,S., Bridgeman,A.M., Brown,A.J., Brown,M.J., Bonnin,D.,
            Bruford,E.A., Buhay,C., Burch,P., Burford,D., Burgess,J.,
            Burrill,W., Burton,J., Bye,J.M., Carder,C., Carrel,L., Chako,J.,
            Chapman,J.C., Chavez,D., Chen,E., Chen,G., Chen,Y., Chen,Z.,
            Chinault,C., Ciccodicola,A., Clark,S.Y., Clarke,G., Clee,C.M.,
            Clegg,S., Clerc-Blankenburg,K., Clifford,K., Cobley,V., Cole,C.G.,
            Conquer,J.S., Corby,N., Connor,R.E., David,R., Davies,J., Davis,C.,
            Davis,J., Delgado,O., Deshazo,D., Dhami,P., Ding,Y., Dinh,H.,
            Dodsworth,S., Draper,H., Dugan-Rocha,S., Dunham,A., Dunn,M.,
            Durbin,K.J., Dutta,I., Eades,T., Ellwood,M., Emery-Cohen,A.,
            Errington,H., Evans,K.L., Faulkner,L., Francis,F., Frankland,J.,
            Fraser,A.E., Galgoczy,P., Gilbert,J., Gill,R., Glockner,G.,
            Gregory,S.G., Gribble,S., Griffiths,C., Grocock,R., Gu,Y.,
            Gwilliam,R., Hamilton,C., Hart,E.A., Hawes,A., Heath,P.D.,
            Heitmann,K., Hennig,S., Hernandez,J., Hinzmann,B., Ho,S., Hoffs,M.,
            Howden,P.J., Huckle,E.J., Hume,J., Hunt,P.J., Hunt,A.R.,
            Isherwood,J., Jacob,L., Johnson,D., Jones,S., de Jong,P.J.,
            Joseph,S.S., Keenan,S., Kelly,S., Kershaw,J.K., Khan,Z.,
            Kioschis,P., Klages,S., Knights,A.J., Kosiura,A., Kovar-Smith,C.,
            Laird,G.K., Langford,C., Lawlor,S., Leversha,M., Lewis,L., Liu,W.,
            Lloyd,C., Lloyd,D.M., Loulseged,H., Loveland,J.E., Lovell,J.D.,
            Lozado,R., Lu,J., Lyne,R., Ma,J., Maheshwari,M., Matthews,L.H.,
            McDowall,J., McLaren,S., McMurray,A., Meidl,P., Meitinger,T.,
            Milne,S., Miner,G., Mistry,S.L., Morgan,M., Morris,S., Muller,I.,
            Mullikin,J.C., Nguyen,N., Nordsiek,G., Nyakatura,G., O'Dell,C.N.,
            Okwuonu,G., Palmer,S., Pandian,R., Parker,D., Parrish,J.,
            Pasternak,S., Patel,D., Pearce,A.V., Pearson,D.M., Pelan,S.E.,
            Perez,L., Porter,K.M., Ramsey,Y., Reichwald,K., Rhodes,S.,
            Ridler,K.A., Schlessinger,D., Schueler,M.G., Sehra,H.K.,
            Shaw-Smith,C., Shen,H., Sheridan,E.M., Shownkeen,R., Skuce,C.D.,
            Smith,M.L., Sotheran,E.C., Steingruber,H.E., Steward,C.A.,
            Storey,R., Swann,R.M., Swarbreck,D., Tabor,P.E., Taudien,S.,
            Taylor,T., Teague,B., Thomas,K., Thorpe,A., Timms,K., Tracey,A.,
            Trevanion,S., Tromans,A.C., d'Urso,M., Verduzco,D., Villasana,D.,
            Waldron,L., Wall,M., Wang,Q., Warren,J., Warry,G.L., Wei,X.,
            West,A., Whitehead,S.L., Whiteley,M.N., Wilkinson,J.E.,
            Willey,D.L., Williams,G., Williams,L., Williamson,A.,
            Williamson,H., Wilming,L., Woodmansey,R.L., Wray,P.W., Yen,J.,
            Zhang,J., Zhou,J., Zoghbi,H., Zorilla,S., Buck,D., Reinhardt,R.,
            Poustka,A., Rosenthal,A., Lehrach,H., Meindl,A., Minx,P.J.,
            Hillier,L.W., Willard,H.F., Wilson,R.K., Waterston,R.H., Rice,C.M.,
            Vaudin,M., Coulson,A., Nelson,D.L., Weinstock,G., Sulston,J.E.,
            Durbin,R., Hubbard,T., Gibbs,R.A., Beck,S., Rogers,J. and
            Bentley,D.R.
  TITLE     The DNA sequence of the human X chromosome
  JOURNAL   Nature 434 (7031), 325-337 (2005)
   PUBMED   15772651
REFERENCE   9  (bases 1 to 8407)
  AUTHORS   Viot,G., Lacombe,D., David,A., Mathieu,M., de Broca,A., Faivre,L.,
            Gigarel,N., Munnich,A., Lyonnet,S., Le Merrer,M. and
            Cormier-Daire,V.
  TITLE     Osteopathia striata cranial sclerosis: non-random X-inactivation
            suggestive of X-linked dominant inheritance
  JOURNAL   Am J Med Genet 107 (1), 1-4 (2002)
   PUBMED   11807859
REFERENCE   10 (bases 1 to 8407)
  AUTHORS   Gear,R. and Savarirayan,R.
  TITLE     Osteopathia Striata with Cranial Sclerosis
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   33856753
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA939151.1, EF186024.1 and
            AL355852.23.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jul 30, 2018 this sequence version replaced NM_152424.3.
            
            Summary: The protein encoded by this gene upregulates
            trancriptional activation by the Wilms tumor protein and interacts
            with many other proteins, including CTNNB1, APC, AXIN1, and AXIN2.
            Defects in this gene are a cause of osteopathia striata with
            cranial sclerosis (OSCS). [provided by RefSeq, May 2010].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EF186024.1, DRR138518.370883.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000374869.8/ ENSP00000364003.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-103               DA939151.1         37-139
            104-3674            EF186024.1         1-3571
            3675-8407           AL355852.23        99298-104030        c
FEATURES             Location/Qualifiers
     source          1..8407
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq11.2"
     gene            1..8407
                     /gene="AMER1"
                     /gene_synonym="FAM123B; OSCS; WTX"
                     /note="APC membrane recruitment protein 1"
                     /db_xref="GeneID:139285"
                     /db_xref="HGNC:HGNC:26837"
                     /db_xref="MIM:300647"
     exon            1..139
                     /gene="AMER1"
                     /gene_synonym="FAM123B; OSCS; WTX"
                     /inference="alignment:Splign:2.1.0"
     exon            140..8407
                     /gene="AMER1"
                     /gene_synonym="FAM123B; OSCS; WTX"
                     /inference="alignment:Splign:2.1.0"
     CDS             238..3645
                     /gene="AMER1"
                     /gene_synonym="FAM123B; OSCS; WTX"
                     /note="adenomatous polyposis coli membrane recruitment 1;
                     Wilms tumor on the X; Wilms tumor gene on the X chromosome
                     protein; protein FAM123B; family with sequence similarity
                     123B; RP11-403E24.2"
                     /codon_start=1
                     /product="APC membrane recruitment protein 1"
                     /protein_id="NP_689637.3"
                     /db_xref="CCDS:CCDS14377.2"
                     /db_xref="GeneID:139285"
                     /db_xref="HGNC:HGNC:26837"
                     /db_xref="MIM:300647"
                     /translation="METQKDEAAQAKGAAASGSTREQTAEKGAKNKAAEATEGPTSEP
                     SSSGPGRLKKTAMKLFGGKKGICTLPSFFGGGRSKGSGKGSSKKGLSKSKTHDGLSEA
                     AHGPEDVVSEGTGFSLPLPELPCQFPSSQSAHGALETGSRCKTSVAGATEKAVAEKFP
                     SMPKPKKGLKGFFSSIRRHRKSKVTGAEQSEPGAKGPERVRARPHEHVSSAPQVPCFE
                     ETFQAPRKENANPQDAPGPKVSPTPEPSPPATEKMACKDPEKPMEACASAHVQPKPAP
                     EASSLEEPHSPETGEKVVAGEVNPPNGPVGDPLSLLFGDVTSLKSFDSLTGCGDIIAE
                     QDMDSMTDSMASGGQRANRDGTKRSSCLVTYQGGGEEMALPDDDDEEEEEEEEVELEE
                     EEEEVKEEEEDDDLEYLWETAQMYPRPNMNLGYHPTTSPGHHGYMLLDPVRSYPGLAP
                     GELLTPQSDQQESAPNSDEGYYDSTTPGFEDDSGEALGLVRRDCLPRDSYSGDALYEF
                     YEPDDSLENSPPGDDCLYDLHGRSSEMFDPFLNFEPFLSSRPPGAMETEEERLVTIQK
                     QLLYWELRREQLEAQEARAREAHAREAHAREAYTREAYGREAYAREAHTWEAHGREAR
                     TREAQAREVRCRETQVRETQARQEKPVLEYQMRPLGPSVMGLAAGVSGTSQISHRGIT
                     SAFPTTASSEPDWRDFRPLEKRYEGTCSKKDQSTCLMQLFQSDAMFEPDMQEANFGGS
                     PRRAYPTYSPPEDPEEEEVEKEGNATVSFSQALVEFTSNGNLFSSMSCSSDSDSSFTQ
                     NLPELPPMVTFDIADVERDGEGKCEENPEFHNDEDLAASLEAFELGYYHKHAFNNYHS
                     RFYQGLPWGVSSLPRYLGLPGLHPRPPPAAMALNRRSRSLDTAETLEMELSNSHLVQG
                     YLESDELQAQQEDSDEEDEEEEEGEWSRDSPLSLYTEPPGAYDWPAWAPCPLPVGPGP
                     AWISPNQLDRPSSQSPYRQATCCIPPMTMSISLSVPESRAPGESGPQLARPSHLHLPM
                     GPCYNLQPQASQSMRARPRDVLLPVDEPSCSSSSGGFSPSPLPQAKPVGITHGIPQLP
                     RVRPEHPQPQPTHYGPSSLDLSKERAEQGASLATSYSSTAMNGNLAK"
     regulatory      8371..8376
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AMER1"
                     /gene_synonym="FAM123B; OSCS; WTX"
                     /note="hexamer: AATAAA"
     polyA_site      8407
                     /gene="AMER1"
                     /gene_synonym="FAM123B; OSCS; WTX"
                     /note="major polyA site"
ORIGIN      
        1 agtgctggag gtgactggag ccgcggcgac ggcggcagca gctgcggtgg cggcgctctc
       61 agccgtggca gccgaggctg cagcccggcc cagggcggct cagcggacaa taaccgggct
      121 aggaacctga ccgggctgga ccccactgtg atgcttcctg actgataatg ttataacagt
      181 gcctggaagc ctgaggctgc atttccagct ggacgtacct cagttgtccc catcatcatg
      241 gagacccaaa aggatgaagc tgctcaggcc aagggagctg cagcctctgg gagtacccgt
      301 gaacaaacag cagaaaaagg agccaagaac aaggcagctg aggcgacaga aggaccaacc
      361 tcagagccat cctcatccgg cccaggtagg ctgaagaaaa ctgccatgaa actctttggt
      421 ggcaagaagg gtatctgtac tctgcctagt ttctttggag ggggacggag caaaggttct
      481 gggaaaggca gctccaagaa aggtctcagc aagagcaaga cccacgatgg cctgagtgaa
      541 gcagcccatg gccctgaaga tgttgtcagt gaaggaactg gcttctccct gcctttgcct
      601 gagttaccct gccaatttcc cagctctcag agtgcccatg gggctttgga gacaggctcc
      661 agatgtaaga catctgtggc tggagccaca gagaaagctg tggctgagaa gtttccctct
      721 atgcccaagc caaagaaagg cctaaaaggc ttttttagca gtatccgccg tcaccggaag
      781 agcaaggtca ctggggctga gcaaagtgag ccaggggcca aggggcctga gagggtcaga
      841 gccaggcctc atgagcacgt gagctcagcc cctcaggtgc cctgctttga ggagaccttc
      901 caagccccta gaaaggaaaa tgctaacccc caagatgccc ctgggccaaa agtttctcca
      961 acaccagaac cttctccacc agctactgag aaaatggcct gtaaagatcc agaaaaaccc
     1021 atggaggcct gtgcctcagc acatgtgcaa cccaagcctg cccctgaagc cagtagccta
     1081 gaggagcccc atagcccaga aacaggggag aaggtagtag caggagaggt aaacccaccc
     1141 aatggccctg tgggggaccc actgagcctc ttgtttgggg atgtgacatc cctgaaaagc
     1201 tttgattcat tgacaggttg tggtgacata atagcagaac aggacatgga cagtatgaca
     1261 gacagcatgg cctctggggg ccagagagca aaccgagatg ggaccaagcg aagttcctgc
     1321 ctggtgacct accaaggagg tggggaggag atggccttgc cagatgatga tgacgaggag
     1381 gaagaagagg aagaagaggt ggaattagag gaggaagaag aggaggttaa ggaggaggaa
     1441 gaagatgatg acttagaata tctgtgggaa actgcccaaa tgtatccacg gcccaatatg
     1501 aacctgggct accatcccac cacatcccca ggccaccacg gctacatgct ccttgaccca
     1561 gttaggtctt atcctggcct agcccctggg gaacttttga ctcctcagag tgaccagcaa
     1621 gaatccgccc ccaatagtga tgaaggttat tatgactcca ccacacctgg atttgaggat
     1681 gattcaggtg aggccctggg gcttgtccgc agggattgtc taccccgaga cagctacagt
     1741 ggagatgccc tatatgagtt ctatgagcca gatgacagcc ttgagaactc tccacctgga
     1801 gatgactgcc tttatgacct ccatggtcga agctctgaga tgtttgaccc cttcttaaac
     1861 tttgagccct ttttgtcctc ccggccacct ggggcaatgg agacagagga agaacggcta
     1921 gtgaccatcc agaaacagtt gttgtattgg gagcttcggc gggagcagct tgaggcccag
     1981 gaggcacgtg cccgagaagc tcatgccagg gaggcccacg ccagggaggc ctatactcga
     2041 gaggcttatg gcagggaagc ctatgccagg gaggcccaca cttgggaagc tcatggcagg
     2101 gaggccagaa cccgagaagc ccaggcccga gaggttcgtt gtagagagac tcaagtccga
     2161 gagacccagg cccggcagga gaagcccgtc ttagagtatc agatgaggcc cttaggccca
     2221 tcagtgatgg gcctggcagc aggggtatca gggacctctc agatttccca ccggggaatt
     2281 acctcagctt tccccaccac tgcaagcagc gagccagact ggagggactt ccgtcctctg
     2341 gagaagcgtt atgaaggaac ctgctccaag aaagatcaaa gtacctgcct gatgcagctc
     2401 ttccagagtg atgccatgtt tgagccagac atgcaagaag caaattttgg aggatctccc
     2461 aggagggcct accctactta ttcaccccct gaagatccag aggaagagga ggttgagaag
     2521 gaagggaatg ccactgtgag tttctcacag gccctggtag agttcaccag caatgggaac
     2581 ctcttttcca gcatgtcctg cagctctgac tctgactcat ctttcactca aaacctccct
     2641 gagctgcctc ccatggtgac ctttgacatc gctgatgtgg aacgggatgg ggaaggcaag
     2701 tgtgaagaga atcctgagtt ccacaatgat gaagatcttg cagcctcctt ggaagccttt
     2761 gagctgggct actatcacaa acatgccttc aacaactacc atagtcgatt ctaccaaggc
     2821 ctgccctggg gtgtgagcag cctccctcga tacttgggac tgcctggcct gcaccctcga
     2881 cctccacctg ctgctatggc cctcaacagg agaagccgct ctctcgacac tgcagagacc
     2941 ctggagatgg agctctccaa ttcccacttg gtccagggct acctcgagtc tgatgagctg
     3001 caggcccagc aggaagattc agatgaggag gacgaagagg aggaagaagg agaatggagc
     3061 cgagacagtc ccctttccct ctatactgaa cccccagggg cctatgattg gcctgcttgg
     3121 gctccctgtc ctcttccagt ggggccaggt cctgcctgga taagccccaa ccagttggac
     3181 aggccttcca gccagtctcc atataggcag gcaacctgtt gcatacctcc tatgaccatg
     3241 tcaatatcac tatcagtgcc agagtcaagg gcacctgggg aatctgggcc tcaactagct
     3301 cgtccctcac acctacacct gcccatgggc ccttgctata acctccagcc acaggcctcc
     3361 cagagcatga gggccaggcc tcgagatgtg ctgctgcctg ttgatgagcc cagttgctct
     3421 tccagttctg gaggcttcag ccccagccct ctaccacagg ccaagcctgt gggcatcacc
     3481 catggcattc ctcagctgcc cagggtccgg cctgagcacc cccagcctca gcccactcac
     3541 tatgggcctt ccagccttga cctgtcaaag gagagggctg agcaaggtgc ctctcttgcc
     3601 accagctact cctccactgc catgaatgga aacctagcca agtagttatt atcaattctg
     3661 gagtagggac acggggcctg agcatgtgaa tggggatcag gggttgggca gaagggtggg
     3721 ggggtggggg ttgttgttta acttgaaaac cctttgggcc aaggaaaact cctgtgagtt
     3781 ttcaccttgg ttttcccact tgcctcttct gccatctatg gccacgttca actcaagtgc
     3841 atctgcccag ggaggctgct gctgctactg cactccagtt tttgctttcg aggtttcttc
     3901 ttgtgaccca cgctgggttt gggatgctgt aattttgtgc ctattccagt tgccaagtta
     3961 gtgattatat atatatatat atatatatat atatatatat atatatatac acacacacac
     4021 acacacacac acacacacat acatacacac acacacacaa acacacatgc acacacatat
     4081 atagcatgca tccttgacac aggctaaacc tttagtgccc cctgcccctt tcccccaatg
     4141 aatgatgaac cactgattag cagccaagtc tgcattgcct cccgcttggg gaagtggggc
     4201 ttatttgcta ttagcaatgt gaaattatgg tacagatcct ccccacccct attccttttg
     4261 gcctggcata gctgccatca ttatatcaga tgactataag ctattcatcc cagctagctg
     4321 tccttagaga ggatcaagta gcgtgtgtct cacagcctat cgctgtggac tttggcatca
     4381 tgaccagatg ggggcctttt aactgtcctg ccatagcttg ggttaaagac aaaaatgcta
     4441 atccccttga tggagtgata aaattcatcc atttgcaaac agaagacaaa acaaggttct
     4501 gttgtggaca ctgccataga gtgttgagct ccggaatgat aggatttttt ttccttcttt
     4561 taagcttgtg actacctgag ttgcagatgc ttttgaattt ttgttctttt ttcctctaaa
     4621 ggaaattttc ataatctcat cacaaggaac acggtctttt aattgagata agggaagaga
     4681 agcaacctaa attaggcttg actatagggg ctggggtttt tgtggagtgt aggggaaaga
     4741 tagaaaatga gggtccattt atcacagcca gcaagaaagg ggatttcaga ccatgcagag
     4801 cttaaggtat ctcagtttct taaaagggaa aactcccttc tcaagcctcc caacacaaaa
     4861 tgttccttaa gccttgccca tcccatcatt tggactctgt aataaggccc caggtttgca
     4921 ctcccaagac aggataccaa aggcatctca gctgctgttc tgggcccttt aaagagcttt
     4981 tatcttccaa ttagccgagt ccttagcatc atcaaatgac agggcttctt ggcagttgca
     5041 gccctttatg gaatgaatgg caccctccct tctcctgcat ccatgcagtt ttcatgttaa
     5101 actaggttgg aaatactggt ggggaagagc ttgctgtcag gaagacattg gaacaggcta
     5161 atcttgccaa gctcaacctc tcattaatca cctgtaggag agggttcctt ttacatgtct
     5221 ttattggaaa tggcatatgg aacatttttt gcttggctgt gttcactcaa attatgatta
     5281 ttgcaccctc gccctcacat cccaccatct ccatgcctgt tgagagaaga agtcaacagg
     5341 gttcttgcca ttcctgagat atggcaattt agcttgggaa cagatgctgt ttgcccccta
     5401 agtcaaggtg actttgtggg caaactgttt cttggccagg ggatgtgtcc atgctggtct
     5461 ttgtcaccat ttctcagatt ggggttggcc cagtgccctt actcacaaat agcagacctg
     5521 gctggtagaa gctgcacccc tggaagcacc acatgaattt gcatggcact aacaccacca
     5581 ctcaagggac ccagaccagc tcttcaggct gactttggct acagatacat tgtgaagaaa
     5641 tgtatctgtt gtggagctgg agaatgaaag agggtgagga aggccctcct taccccaagc
     5701 acagcattcc aagagagaag tgcccaaatt ccaactttct ggcctcttac caaagctgct
     5761 ctgggatggt ggggctgaaa ttggcctggc tatttggcag gaagtaaaca ctcaccaaat
     5821 gcctttacaa cagggaagaa cccacctctt gcctcttctt tcagggcagc ttgagctcct
     5881 gtctacatgt ggactgtgcc agccacagca ggcggtagag ccatcaccag cctccagacc
     5941 ctgctggaga agggccacca gcctatctgg gaagagaaaa aggaggtggc caacctttgg
     6001 ctaagggctg agagctgccc tcaggatcag gaaggcagca tgaggcattg tctctgaggg
     6061 gcactggcat agccctgaca cactttgttg ggctttggaa ggtcacacac cacagagtag
     6121 ggcctggggc tgagtgagag ttcatggttt ggacagcctt gctgcctgca agtcccttca
     6181 ctgccctagc cagcccagga ataggatcct taccacctga agtggggcag ccaggatatg
     6241 tggctgcaga agcaggttcc tttgaatttc tagagctaag cagtttgtgg ctgaaaccca
     6301 atggcagcct aggggaggaa ggaagacctg tctggggacc catggtgttg acagcacagg
     6361 gtgacattgg gagagccagc caagcttcag gcctgtttcc aaacacagac tagtgctggc
     6421 ttgggtggca gcaaatacac cccagaccac taaaactgtt gatatcatgt ccacacctcc
     6481 aagagtacta ctagatgaac aacatgtctc cattgcaagg tcctggaata atgcttttgg
     6541 atgtgatttt aagggaaaat gggacttagt ggaagtagct gccttcatct tattacccaa
     6601 taggcctagt gccagacagc tgtgatcctg agaattccca gatgagactg gtccttggag
     6661 gaccagtgaa tgtggataga gaaggccacc tagtaggcct ctcccagggc caggatgcca
     6721 gtttcttggt gctttagagt ctccaaggaa gcactgcctt ggggcccagt tcaagccagc
     6781 cctacctatc tggcctcagc ttgggtacca ggccacaaga ctgcccggat aatgggttca
     6841 gtaaacacca gtctctctcc ctccttagtc ctatgccaag aagggaagta gcttcacaca
     6901 ctcaggaggc tgggtgtgta cccttcatcc tgctgccagc agctttcctt acctgtgcca
     6961 gtgccaccac cccacctgcc ctcagccccc aggcccagtt gctagaggga ggcatggggt
     7021 ttagcaggga gacttcaagt ttacccatgt gtatgttgca ttcaagttgt ttttaatata
     7081 tatatatata tatatcaaaa gtcaattatc tatttttata ttgtttgcat tttatattca
     7141 tggaaaacaa tgtttatcaa ttgttctttt ctgatttatt tttaagcggt gctgtttaca
     7201 gttttaaaca aaacaaagat gacacaaaaa ttgctgctgc ttgtgcagag ggctggcatc
     7261 tcaactgcct agggccctcc ctctttcccc tctccctacc ttagctgtac cacactgtcc
     7321 gttccctccc cccatgatag ggacagcaga agatgcccag gtactgaaga caaacgccaa
     7381 gtgactcagg cgtgtgggac ctggtagaca gcttgtcatt ttctggcttt attttatgtt
     7441 tttctttctt ttgaaccctt cttccccatg tctagaccct ctgagagtac tcatgtgtct
     7501 tatttttttt cctcgttatg ttttgtgctt tagttttgaa atcagagtgc ccatgttgtg
     7561 ctatttgtct tgttgagttt ctccaaagga gcattttggg aacattctcc acccaatggg
     7621 gaaggaaggg ggtttcaacc atgtcccctt tccccaagaa gctgtgggag acagatgaca
     7681 atgatgcttg cactatcagg aatttaccat ctcgttgagg aaacccttgc ataggacttc
     7741 ctaggagctc agttgattgt gacattttcc cagaagagag gctttgggga actccttcac
     7801 ccctcttgct gctttggggg aatctcctcc cctaccacca ttccccctcc cctgccctgc
     7861 tccagctcac acgtggatgg tactttgggg aacagtgact ttcagggtga tggcttgctg
     7921 gcctgatccc tgactttcca atcctatagt cactccaaag aagaaaatcc acatggtatg
     7981 tatatgtgtg agggttgggt ctattgagag gtgggacttt aattattgtt attattattg
     8041 gtgcttattt ttcctgctgt atatgggggt gggggtgggg gggaataaaa caggaatgta
     8101 tatttaggtc atgttaaata aaatggctgg aggaaggagg gaagaaatta ggaactgcag
     8161 tacatcatca ccccaggtgt aagagttatt tctctcccag tgcagaaagg ggagtgtggc
     8221 ccctggctgt acctgagctt cctcttctcc atccctgaga catcacagaa tcacccgggt
     8281 tctgacctct ccacttctag ctctcactga catctggata tcttgatgca ctgcctgttt
     8341 gtaacaacat atacttcttt gctgaatgct aataaacttc tgggttcaag agggaacccc
     8401 tcagtca
//
