LOCUS       NM_020919               6675 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens alsin Rho guanine nucleotide exchange factor ALS2
            (ALS2), transcript variant 1, mRNA.
ACCESSION   NM_020919
VERSION     NM_020919.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Miceli,M., Cannariato,M., Tortarolo,R., Pallante,L., Zizzi,E.A. and
            Deriu,M.A.
  TITLE     Conformational Dynamics and Molecular Characterization of Alsin
            MORN Monomer and Dimeric Assemblies
  JOURNAL   Proteins 92 (11), 1343-1353 (2024)
   PUBMED   39023312
  REMARK    GeneRIF: Conformational Dynamics and Molecular Characterization of
            Alsin MORN Monomer and Dimeric Assemblies.
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Silva,P., Hernandez,N., Tapia,H., Gaete-Ramirez,B., Torres,P.,
            Flores,T., Herrera,D., Caceres-Verschae,A., Acuna,R.A.,
            Varas-Godoy,M. and Torres,V.A.
  TITLE     Tumor-derived hypoxic small extracellular vesicles promote
            endothelial cell migration and tube formation via
            ALS2/Rab5/beta-catenin signaling
  JOURNAL   FASEB J 38 (11), e23716 (2024)
   PUBMED   38847490
  REMARK    GeneRIF: Tumor-derived hypoxic small extracellular vesicles promote
            endothelial cell migration and tube formation via
            ALS2/Rab5/beta-catenin signaling.
REFERENCE   3  (bases 1 to 6675)
  AUTHORS   Shimakura,K., Sato,K., Mitsui,S., Ono,S., Otomo,A. and Hadano,S.
  TITLE     The N-terminal intrinsically disordered region mediates
            intracellular localization and self-oligomerization of ALS2
  JOURNAL   Biochem Biophys Res Commun 569, 106-111 (2021)
   PUBMED   34243065
  REMARK    GeneRIF: The N-terminal intrinsically disordered region mediates
            intracellular localization and self-oligomerization of ALS2.
REFERENCE   4  (bases 1 to 6675)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 6675)
  AUTHORS   Hadano,S., Hand,C.K., Osuga,H., Yanagisawa,Y., Otomo,A.,
            Devon,R.S., Miyamoto,N., Showguchi-Miyata,J., Okada,Y.,
            Singaraja,R., Figlewicz,D.A., Kwiatkowski,T., Hosler,B.A.,
            Sagie,T., Skaug,J., Nasir,J., Brown,R.H. Jr., Scherer,S.W.,
            Rouleau,G.A., Hayden,M.R. and Ikeda,J.E.
  TITLE     A gene encoding a putative GTPase regulator is mutated in familial
            amyotrophic lateral sclerosis 2
  JOURNAL   Nat Genet 29 (2), 166-173 (2001)
   PUBMED   11586298
REFERENCE   6  (bases 1 to 6675)
  AUTHORS   Yang,Y., Hentati,A., Deng,H.X., Dabbagh,O., Sasaki,T., Hirano,M.,
            Hung,W.Y., Ouahchi,K., Yan,J., Azim,A.C., Cole,N., Gascon,G.,
            Yagmour,A., Ben-Hamida,M., Pericak-Vance,M., Hentati,F. and
            Siddique,T.
  TITLE     The gene encoding alsin, a protein with three guanine-nucleotide
            exchange factor domains, is mutated in a form of recessive
            amyotrophic lateral sclerosis
  JOURNAL   Nat Genet 29 (2), 160-165 (2001)
   PUBMED   11586297
REFERENCE   7  (bases 1 to 6675)
  AUTHORS   Hadano,S., Nichol,K., Brinkman,R.R., Nasir,J., Martindale,D.,
            Koop,B.F., Nicholson,D.W., Scherer,S.W., Ikeda,J.E. and Hayden,M.R.
  TITLE     A yeast artificial chromosome-based physical map of the juvenile
            amyotrophic lateral sclerosis (ALS2) critical region on human
            chromosome 2q33-q34
  JOURNAL   Genomics 55 (1), 106-112 (1999)
   PUBMED   9889004
REFERENCE   8  (bases 1 to 6675)
  AUTHORS   Hosler,B.A., Sapp,P.C., Berger,R., O'Neill,G., Bejaoui,K.,
            Hamida,M.B., Hentati,F., Chin,W., McKenna-Yasek,D., Haines,J.L.,
            Patterson,D., Horvitz,H.R., Brown,R.H. Jr. and Day,C.B.
  TITLE     Refined mapping and characterization of the recessive familial
            amyotrophic lateral sclerosis locus (ALS2) on chromosome 2q33
  JOURNAL   Neurogenetics 2 (1), 34-42 (1998)
   PUBMED   9933298
REFERENCE   9  (bases 1 to 6675)
  AUTHORS   Siddique,N. and Siddique,T.
  TITLE     Amyotrophic Lateral Sclerosis Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301623
REFERENCE   10 (bases 1 to 6675)
  AUTHORS   Orrell,R.W.
  TITLE     ALS2-Related Disorder
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301421
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK291029.1, AF391100.1 and
            BM978733.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_020919.3.
            
            Summary: The protein encoded by this gene contains an
            ATS1/RCC1-like domain, a RhoGEF domain, and a vacuolar protein
            sorting 9 (VPS9) domain, all of which are guanine-nucleotide
            exchange factors that activate members of the Ras superfamily of
            GTPases. The protein functions as a guanine nucleotide exchange
            factor for the small GTPase RAB5. The protein localizes with RAB5
            on early endosomal compartments, and functions as a modulator for
            endosomal dynamics. Mutations in this gene result in several forms
            of juvenile lateral sclerosis and infantile-onset ascending spastic
            paralysis. Multiple transcript variants encoding different isoforms
            have been found for this gene. [provided by RefSeq, Oct 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1), also known as the LF
            or long form.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB053305.1, AF391100.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000264276.11/ ENSP00000264276.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3429              AK291029.1         24-3452
            3430-6388           AF391100.1         3436-6394
            6389-6675           BM978733.1         22-308              c
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q33.1"
     gene            1..6675
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /note="alsin Rho guanine nucleotide exchange factor ALS2"
                     /db_xref="GeneID:57679"
                     /db_xref="HGNC:HGNC:443"
                     /db_xref="MIM:606352"
     exon            1..57
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            58..137
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..5091
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /note="isoform 1 is encoded by transcript variant 1;
                     alsin; amyotrophic lateral sclerosis 2 protein; ALS2,
                     alsin Rho guanine nucleotide exchange factor; amyotrophic
                     lateral sclerosis 2 chromosomal region candidate gene 6
                     protein; amyotrophic lateral sclerosis 2 (juvenile)"
                     /codon_start=1
                     /product="alsin isoform 1"
                     /protein_id="NP_065970.2"
                     /db_xref="CCDS:CCDS42800.1"
                     /db_xref="GeneID:57679"
                     /db_xref="HGNC:HGNC:443"
                     /db_xref="MIM:606352"
                     /translation="MDSKKRSSTEAEGSKERGLVHIWQAGSFPITPERLPGWGGKTVL
                     QAALGVKHGVLLTEDGEVYSFGTLPWRSGPVEICPSSPILENALVGQYVITVATGSFH
                     SGAVTDNGVAYMWGENSAGQCAVANQQYVPEPNPVSIADSEASPLLAVRILQLACGEE
                     HTLALSISREIWAWGTGCQLGLITTAFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQ
                     CLPSQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLTESQAENHASTAL
                     SPSTETLDRQEEVFENTLVANDQSVATELNAVSAQITSSDAMSSQQNVMGTTEISSAR
                     NIPSYPDTQAVNEYLRKLSDHSVREDSEHGEKPVPSQPLLEEAIPNLHSPPTTSTSAL
                     NSLVVSCASAVGVRVAATYEAGALSLKKVMNFYSTTPCETGAQAGSSAIGPEGLKDSR
                     EEQVKQESMQGKKSSSLVDIREEETEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVV
                     LTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEA
                     GGYHSLALTAKSQVYSWGSNTFGQLGHSDFPTTVPRLAKISSENGVWSIAAGRDYSLF
                     LVDTEDFQPGLYYSGRQDPTEGDNLPENHSGSKTPVLLSCSKLGYISRVTAGKDSYLA
                     LVDKNIMGYIASLHELATTERRFYSKLSDIKSQILRPLLSLENLGTTTTVQLLQEVAS
                     RFSKLCYLIGQHGASLSSFLHGVKEARSLVILKHSSLFLDSYTEYCTSITNFLVMGGF
                     QLLAKPAIDFLNKNQELLQDLSEVNDENTQLMEILNTLFFLPIRRLHNYAKVLLKLAT
                     CFEVASPEYQKLQDSSSCYECLALHLGRKRKEAEYTLGFWKTFPGKMTDSLRKPERRL
                     LCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEEAGGVN
                     GLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGMSDLPPYGSGSSVQRQEPPI
                     SRSAKYTFYKDPRLKDATYDGRWLSGKPHGRGVLKWPDGKMYSGMFRNGLEDGYGEYR
                     IPNKAMNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSS
                     SPSMFIGQWVMDKKAGYGVFDDITRGEKYMGMWQDDVCQGNGVVVTQFGLYYEGNFHL
                     NKMMGNGVLLSEDDTIYEGEFSDDWTLSGKGTLTMPNGDYIEGYFSGEWGSGIKITGT
                     YFKPSLYESDKDRPKVFRKLGNLAVPADEKWKAVFDECWRQLGCEGPGQGEVWKAWDN
                     IAVALTTSRRQHRDSPEILSRSQTQTLESLEFIPQHVGAFSVEKYDDIRKYLIKACDT
                     PLHPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEE
                     GSTIPLSAPLPTERKSFCTGKSDSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFMLYA
                     LDNDREEDIYWECVLRLNKQPDIALLGFLGVQRKFWPATLSILGESKKVLPTTKDACF
                     ASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLHEDFLWSMDDLFPVFLYVVL
                     RARIRNLGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN"
     exon            138..292
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            293..1230
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1588
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1757
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1854
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            1855..1932
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            1933..2115
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            2116..2287
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            2288..2468
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            2469..2534
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            2535..2697
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            2698..2829
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            2830..2958
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            2959..3029
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            3030..3096
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            3097..3299
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            3300..3365
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            3366..3464
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            3465..3629
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            3630..3741
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            3742..3819
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            3820..3953
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            3954..4121
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            4122..4239
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            4240..4397
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            4398..4520
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            4521..4697
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            4698..4743
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            4744..4805
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            4806..4955
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            4956..5052
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     exon            5053..6675
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6369..6374
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /note="hexamer: AATAAA"
     polyA_site      6389
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /note="major polyA site"
     regulatory      6655..6660
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
                     /note="hexamer: AATAAA"
     polyA_site      6675
                     /gene="ALS2"
                     /gene_synonym="ALS2CR6; ALSJ; IAHSP; PLSJ"
ORIGIN      
        1 actgggttgc caagctcgcg ccggatgcgg agcgcggtgc tgccggtgga gcttcaggtc
       61 ttgatagact ttctgtaaag aaggaatgat ttggtgatgg agtgttccca ctgaccgatg
      121 gactcaaaga agagaagctc aacagaggca gaaggatcca aggaaagagg cctggtccat
      181 atctggcagg caggatcctt tcccataaca ccagagagat tgccaggctg gggaggaaag
      241 actgttttgc aggcagccct cggagtgaaa catggagttc ttctgactga agatggtgag
      301 gtctacagct ttgggactct tccctggaga agtggaccag tggagatttg tccaagtagc
      361 cccattctag aaaatgccct ggttgggcaa tatgttatta ctgtggcaac aggaagcttc
      421 catagtggag cagtgacaga caatggtgtc gcgtacatgt ggggagagaa ttctgctggc
      481 cagtgtgcag tagccaacca gcagtatgtg ccggaaccaa atcctgtcag cattgctgat
      541 tctgaggcca gccctttgtt agcagtcagg attttacagt tggcgtgtgg cgaggagcac
      601 actctggcat tgtcaataag cagagagatt tgggcatggg gtaccggttg tcagttgggt
      661 ctcattacca ctgccttccc agtgacaaag ccgcaaaagg tagaacatct tgctgggcga
      721 gtggtgcttc aagttgcctg tggtgctttc cacagcttag cccttgtaca atgcctccct
      781 tcccaggatc tgaagccagt cccagaacga tgcaaccagt gcagccagct cttgattact
      841 atgactgaca aagaagacca tgtgattata tcagacagtc attgttgccc attaggtgtg
      901 acactgacag aatctcaggc agaaaaccat gccagcactg ctctcagccc ctccactgaa
      961 acccttgaca ggcaggaaga agtatttgag aacactcttg tagcaaatga tcagtctgtt
     1021 gctactgaac tgaatgcagt aagtgctcag atcacaagca gcgatgccat gtcctctcaa
     1081 caaaatgtca tgggaacaac tgaaatttcc tctgccagaa acataccatc ataccctgac
     1141 acccaagcag tcaatgaata cctacggaaa ctgtcagatc attcagtaag agaggactca
     1201 gagcatggtg aaaagccagt gccatctcag cctcttttag aagaagcaat tcctaatctc
     1261 cacagcccgc ctaccacaag cacctcagcc ctaaacagcc tggtggtctc ttgtgcatct
     1321 gctgttggtg tgagagtggc tgctacttat gaagctggtg ccttgtcact gaagaaagtt
     1381 atgaactttt atagtacaac cccttgtgaa actggagctc aggcaggcag tagtgccatt
     1441 ggccccgaag gtttgaaaga tagcagggaa gaacaggtta aacaggaatc aatgcaagga
     1501 aagaaaagtt caagtcttgt ggatatcaga gaagaagaaa cagagggagg cagtcgaaga
     1561 ctctccctcc ctggattgtt gtcacaagtt tcccccaggc tcttaagaaa ggctgcacgg
     1621 gtgaaaacga ggacagtggt tctgaccccc acatacagtg gagaagcaga tgcgctcctg
     1681 ccttctctga gaacagaagt gtggacctgg gggaaaggga aggaagggca gctggggcac
     1741 ggcgatgttc tgcctaggct tcaaccgttg tgtgtaaaat gtctggatgg caaagaagta
     1801 atccatctgg aggcaggtgg ttaccattct cttgcactta ctgcgaaatc ccaggtttac
     1861 tcatggggta gcaatacctt tggtcaactt gggcattccg attttccaac aacagttcct
     1921 cgtcttgcaa agataagcag tgaaaatgga gtctggagca tagctgcagg cagggattat
     1981 tccctgtttt tagtggatac agaagacttc cagcctgggt tatattacag tggccgacag
     2041 gaccctacag aaggtgacaa ccttccagag aatcacagtg gttctaagac tccagtactt
     2101 ctctcctgta gtaagcttgg atatataagc agagtgacag caggaaaaga tagctattta
     2161 gccttggtgg ataaaaacat tatggggtat attgccagtc tccacgagtt agctactaca
     2221 gaaagacgat tctattcaaa actaagtgat atcaaatctc agattctcag gcctcttctc
     2281 agtttagaaa atttgggcac tacaactaca gtccagctgt tgcaggaggt ggctagccga
     2341 ttcagcaagc tgtgttacct cattggtcag catggagcct cattgagcag cttccttcat
     2401 ggggtaaagg aagccaggag tttggtcatc ctgaagcatt caagtctctt cttggatagt
     2461 tatacagagt attgcacatc tattacaaat ttcctggtta tgggaggatt ccagcttctt
     2521 gctaagcctg ccattgattt cctaaataaa aaccaagagc tgttgcaaga tttgtcagaa
     2581 gtgaatgacg aaaacactca gttgatggaa atactgaata ctttgttttt cttgccaatc
     2641 agacgacttc ataattacgc aaaagttttg ctaaagcttg ctacttgttt tgaagtggca
     2701 tctccagaat atcagaaact gcaggattcc agttcttgtt atgagtgtct tgctctccat
     2761 ctcggcagga aaaggaagga agcagaatac acactgggct tctggaagac cttccccgga
     2821 aaaatgacgg attccttgag gaagccagag cgtcgactgc tgtgtgagag tagtaaccga
     2881 gccctgtctc tgcagcatgc tgggaggttt tccgtgaatt ggttcattct ctttaatgat
     2941 gccctggtcc atgcccagtt ctccacgcac catgttttcc ctctggccac gctgtgggca
     3001 gagccactgt ctgaagaagc tggtggtgtg aatggcttaa agataactac acctgaggag
     3061 cagttcactc tcatttcatc tacaccccag gaaaagacaa agtggctacg agctataagc
     3121 caagccgtag atcaggcttt gagagggatg tctgatctcc ccccttatgg aagtggtagc
     3181 agtgttcaga gacaggaacc acccatttca cgcagtgcca aatatacttt ctacaaggat
     3241 cctcgcctaa aggatgccac ctatgatgga cgctggcttt cagggaagcc tcatggcaga
     3301 ggggttttga agtggcctga tggaaagatg tattctggca tgttcaggaa tggcttggaa
     3361 gatgggtatg gagaatacag aatcccaaac aaggcaatga acaaagaaga ccattatgtg
     3421 ggccattgga aagaaggaaa aatgtgcggt caaggagtct acagctatgc ttctggtgaa
     3481 gtatttgagg gctgttttca agataatatg cgtcatggtc atggtcttct acgaagtggg
     3541 aaattgacgt cctcttctcc tagtatgttc attggccagt gggtaatgga taagaaagca
     3601 ggatatggtg tctttgatga tatcactagg ggggaaaagt atatgggaat gtggcaagat
     3661 gatgtgtgtc aagggaatgg tgtggtggtt acccagtttg gattatacta cgagggcaac
     3721 tttcacctta ataaaatgat gggaaatggg gttttgcttt ccgaagatga tactatctat
     3781 gaaggagaat tttcagatga ctggactctt agtggaaagg gaacactgac tatgccaaat
     3841 ggagactaca ttgaaggtta ttttagtgga gaatggggat ctgggataaa aatcactgga
     3901 acctacttca aacctagtct atatgagagt gataaagaca gacctaaagt tttcaggaag
     3961 ctaggaaacc tggcagtgcc agctgatgag aagtggaaag cggtgtttga cgaatgttgg
     4021 cgccaactgg gctgtgaggg cccaggccaa ggggaagttt ggaaagcatg ggacaatatt
     4081 gctgtggcct tgaccaccag tcggcgccag cacagagaca gtccagaaat actgagtcgt
     4141 tcacagactc agacactaga gagtttggaa ttcattccac agcatgttgg tgccttctct
     4201 gtggagaaat atgatgacat caggaaatat ttaataaagg cctgtgacac tcctctgcac
     4261 cccctgggca ggcttgtgga gacactggtt gcagtgtata gaatgacata cgtgggcgta
     4321 ggagccaacc gcaggttatt gcaggaggct gtaaaggaga ttaagtccta tcttaagcga
     4381 attttccagc tggtgaggtt cttatttcct gagctgcctg aagaaggcag cacaattcct
     4441 ctctctgctc ctctgccaac cgaaaggaag tctttttgca ctgggaagtc agattcccga
     4501 tctgaatcac cagagccagg ttatgtagta acgagttctg gattattgct tcctgtgctg
     4561 ctacctcggc tctacccacc gctgtttatg ctttatgctt tggataatga tcgcgaggaa
     4621 gacatttact gggaatgtgt ccttcgacta aataagcagc cagatattgc tctcctgggc
     4681 tttcttgggg tgcagaggaa attttggcca gcaaccttgt caatccttgg agagagtaaa
     4741 aaggttttgc caaccacgaa agatgcttgt tttgcctcag cagtagaatg tctgcagcag
     4801 atcagcacaa catttacccc atcagacaaa cttaaggtca tccagcagac ttttgaggag
     4861 atctctcaga gtgtcctggc gtcactccac gaagacttct tgtggtccat ggatgacttg
     4921 tttcctgttt tcttatatgt ggtgctacgg gccaggatta ggaatttagg ctctgaggta
     4981 cacctcattg aggatctaat ggacccctat cttcagcatg gggaacaggg tataatgttc
     5041 accaccttga aggcatgtta ctaccagatt cagcgtgaga agcttaacta ggctgcataa
     5101 cagcttgaaa actggattat ctactacaga gtgttataac accatctgga gtcttcctgt
     5161 agtggcaaaa aagaacagtg ttgaaattgg aaaggacttt gtgttattta ggttgttaga
     5221 atgagcctta ccaataataa gagccctgag cccagaaaaa aggactgtat agtttaaagg
     5281 gaggattgaa agggaggtaa aaaatcagat tagaccagtt cttggcctat gataagttcc
     5341 aaaaatacca tttatctact atttgaaaaa agaagaggat atcccttcct acagtaaagg
     5401 gtatgtcagc tacatgaagt tgtaagaaaa gcttccagta gagcttctta tattaaagaa
     5461 gttgatggat atttttgaat ttctggtttg cctgaatcca cctgcagtta ccccgatccg
     5521 tttgcaagaa ccagatcgta cttgaaacta tagtggccac actctgcctt cctgagtccc
     5581 ttccagtcat gtgtgcatca tgtctctttg ccaagggagg ggagaaagga acttttaaac
     5641 tgcagtttta actttttcta agctgtttct tgatgggaga ggttctgtgc aaaactacca
     5701 cattctgtcc ccaaaatgtg gaatgcatcc aaataggagt cttctgcctc ttaacttaaa
     5761 agaacatagg aattttgttt ttggtttctt tatcatgcta cagagagtga atacactgga
     5821 attcagacac cgactctgag ctgctaggaa cctcatttgt ccatgtgcaa acgctgtatt
     5881 ccaaggcctg tgaatggcag cctgaggaag ttttgcatgc aggctgtgtt ttcgagcagg
     5941 actaacaact gggaaataag caaaaaactg catcgatccc cagcctggtg ttgttcttcc
     6001 ctatacttca cactgaactc aggatgggaa gaaaaaggaa acaagctttg gctttttcca
     6061 tctcaaaagt attgtggcac ctcaacattt cagtgttttg ctttttaaaa aatgccctat
     6121 tgtaagttgt tggtttatac tgtataagta acactagtag ctgttttgaa taacataggt
     6181 gctcttcctc atctcatctc ctacaccgtg gtgagcatac agagtgtcct gatttgtgtt
     6241 aagtgactga gaagatgtta attacttttg aaaaaggatc atggtttttg ctctacttta
     6301 taatcaagac aagtgtttat taaaatactg ttttggaatg ttggctgtaa tgtaacagca
     6361 attttcataa taaaaggcat tcatctttat gtggttgttt tatgattgta gaagaaatga
     6421 acagggcagc ctaaaagatg aaaggaaacc cagacttagt atttctttag cccacattgt
     6481 aatccatgcc tctaagtatt ggcctcaaaa gtccctctgg ggttttagaa atctctgtct
     6541 ttaaaataaa cgtaaaccaa tagttatagc accctttcta gcaaagagca aattaacttt
     6601 ctgaccccaa taacccaagg acacgggtct ccaaagaaat ctattatttc tttgaataaa
     6661 aagcatattt tgata
//
