LOCUS       NM_052947               7437 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens alpha kinase 2 (ALPK2), mRNA.
ACCESSION   NM_052947
VERSION     NM_052947.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7437)
  AUTHORS   Yan,J.S., Chen,Q., Li,Y.L. and Gao,Y.Q.
  TITLE     Hsa_circ_0065217 promotes growth and metastasis of renal cancer
            through regulating the miR-214-3p-ALPK2 axis
  JOURNAL   Cell Cycle 20 (23), 2519-2530 (2021)
   PUBMED   34705617
  REMARK    GeneRIF: Hsa_circ_0065217 promotes growth and metastasis of renal
            cancer through regulating the miR-214-3p-ALPK2 axis.
REFERENCE   2  (bases 1 to 7437)
  AUTHORS   Wang,Y., Wu,J., Luo,W., Zhang,H., Shi,G., Shen,Y., Zhu,Y., Ma,C.,
            Dai,B., Ye,D. and Zhu,Y.
  TITLE     ALPK2 acts as tumor promotor in development of bladder cancer
            through targeting DEPDC1A
  JOURNAL   Cell Death Dis 12 (7), 661 (2021)
   PUBMED   34210956
  REMARK    GeneRIF: ALPK2 acts as tumor promotor in development of bladder
            cancer through targeting DEPDC1A.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7437)
  AUTHORS   McIntosh,A.T., Wei,R., Ahn,J., Aouizerat,B.E., Kassaye,S.G.,
            Augenbraun,M.H., Price,J.C., French,A.L., Gange,S.J., Anastos,K.M.
            and Goldman,R.
  TITLE     A genomic variant of ALPK2 is associated with increased liver
            fibrosis risk in HIV/HCV coinfected women
  JOURNAL   PLoS One 16 (3), e0247277 (2021)
   PUBMED   33705408
  REMARK    GeneRIF: A genomic variant of ALPK2 is associated with increased
            liver fibrosis risk in HIV/HCV coinfected women.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7437)
  AUTHORS   Jiang,J., Han,P., Qian,J., Zhang,S., Wang,S., Cao,Q. and Shao,P.
  TITLE     Knockdown of ALPK2 blocks development and progression of renal cell
            carcinoma
  JOURNAL   Exp Cell Res 392 (2), 112029 (2020)
   PUBMED   32330508
  REMARK    GeneRIF: Knockdown of ALPK2 blocks development and progression of
            renal cell carcinoma.
REFERENCE   5  (bases 1 to 7437)
  AUTHORS   Hofsteen,P., Robitaille,A.M., Strash,N., Palpant,N., Moon,R.T.,
            Pabon,L. and Murry,C.E.
  TITLE     ALPK2 Promotes Cardiogenesis in Zebrafish and Human Pluripotent
            Stem Cells
  JOURNAL   iScience 2, 88-100 (2018)
   PUBMED   29888752
REFERENCE   6  (bases 1 to 7437)
  AUTHORS   Nishi,K., Luo,H., Nakabayashi,K., Doi,K., Ishikura,S., Iwaihara,Y.,
            Yoshida,Y., Tanisawa,K., Arai,T., Mori,S., Sawabe,M., Muramatsu,M.,
            Tanaka,M., Sakata,T., Shirasawa,S. and Tsunoda,T.
  TITLE     An Alpha-kinase 2 Gene Variant Disrupts Filamentous Actin
            Localization in the Surface Cells of Colorectal Cancer Spheroids
  JOURNAL   Anticancer Res 37 (7), 3855-3862 (2017)
   PUBMED   28668886
  REMARK    GeneRIF: Results showed that p.Q1853E variant of ALPK2, which had
            been accumulating in the Japanese population, induced a metastatic
            phenotype of colorectal tumors by disrupting ALPK2 function.
REFERENCE   7  (bases 1 to 7437)
  AUTHORS   Yoshida,Y., Tsunoda,T., Doi,K., Fujimoto,T., Tanaka,Y., Ota,T.,
            Ogawa,M., Matsuzaki,H., Kuroki,M., Iwasaki,A. and Shirasawa,S.
  TITLE     ALPK2 is crucial for luminal apoptosis and DNA repair-related gene
            expression in a three-dimensional colonic-crypt model
  JOURNAL   Anticancer Res 32 (6), 2301-2308 (2012)
   PUBMED   22641666
  REMARK    GeneRIF: ALPK2 is crucial for luminal apoptosis and expression of
            DNA repair-related genes, possibly in the transition of normal
            colonic crypt to adenoma.
REFERENCE   8  (bases 1 to 7437)
  AUTHORS   Sawcer,S., Hellenthal,G., Pirinen,M., Spencer,C.C.,
            Patsopoulos,N.A., Moutsianas,L., Dilthey,A., Su,Z., Freeman,C.,
            Hunt,S.E., Edkins,S., Gray,E., Booth,D.R., Potter,S.C., Goris,A.,
            Band,G., Oturai,A.B., Strange,A., Saarela,J., Bellenguez,C.,
            Fontaine,B., Gillman,M., Hemmer,B., Gwilliam,R., Zipp,F.,
            Jayakumar,A., Martin,R., Leslie,S., Hawkins,S., Giannoulatou,E.,
            D'alfonso,S., Blackburn,H., Martinelli Boneschi,F., Liddle,J.,
            Harbo,H.F., Perez,M.L., Spurkland,A., Waller,M.J., Mycko,M.P.,
            Ricketts,M., Comabella,M., Hammond,N., Kockum,I., McCann,O.T.,
            Ban,M., Whittaker,P., Kemppinen,A., Weston,P., Hawkins,C.,
            Widaa,S., Zajicek,J., Dronov,S., Robertson,N., Bumpstead,S.J.,
            Barcellos,L.F., Ravindrarajah,R., Abraham,R., Alfredsson,L.,
            Ardlie,K., Aubin,C., Baker,A., Baker,K., Baranzini,S.E.,
            Bergamaschi,L., Bergamaschi,R., Bernstein,A., Berthele,A.,
            Boggild,M., Bradfield,J.P., Brassat,D., Broadley,S.A., Buck,D.,
            Butzkueven,H., Capra,R., Carroll,W.M., Cavalla,P., Celius,E.G.,
            Cepok,S., Chiavacci,R., Clerget-Darpoux,F., Clysters,K., Comi,G.,
            Cossburn,M., Cournu-Rebeix,I., Cox,M.B., Cozen,W., Cree,B.A.,
            Cross,A.H., Cusi,D., Daly,M.J., Davis,E., de Bakker,P.I.,
            Debouverie,M., D'hooghe,M.B., Dixon,K., Dobosi,R., Dubois,B.,
            Ellinghaus,D., Elovaara,I., Esposito,F., Fontenille,C., Foote,S.,
            Franke,A., Galimberti,D., Ghezzi,A., Glessner,J., Gomez,R.,
            Gout,O., Graham,C., Grant,S.F., Guerini,F.R., Hakonarson,H.,
            Hall,P., Hamsten,A., Hartung,H.P., Heard,R.N., Heath,S., Hobart,J.,
            Hoshi,M., Infante-Duarte,C., Ingram,G., Ingram,W., Islam,T.,
            Jagodic,M., Kabesch,M., Kermode,A.G., Kilpatrick,T.J., Kim,C.,
            Klopp,N., Koivisto,K., Larsson,M., Lathrop,M., Lechner-Scott,J.S.,
            Leone,M.A., Leppa,V., Liljedahl,U., Bomfim,I.L., Lincoln,R.R.,
            Link,J., Liu,J., Lorentzen,A.R., Lupoli,S., Macciardi,F., Mack,T.,
            Marriott,M., Martinelli,V., Mason,D., McCauley,J.L., Mentch,F.,
            Mero,I.L., Mihalova,T., Montalban,X., Mottershead,J., Myhr,K.M.,
            Naldi,P., Ollier,W., Page,A., Palotie,A., Pelletier,J., Piccio,L.,
            Pickersgill,T., Piehl,F., Pobywajlo,S., Quach,H.L., Ramsay,P.P.,
            Reunanen,M., Reynolds,R., Rioux,J.D., Rodegher,M., Roesner,S.,
            Rubio,J.P., Ruckert,I.M., Salvetti,M., Salvi,E., Santaniello,A.,
            Schaefer,C.A., Schreiber,S., Schulze,C., Scott,R.J., Sellebjerg,F.,
            Selmaj,K.W., Sexton,D., Shen,L., Simms-Acuna,B., Skidmore,S.,
            Sleiman,P.M., Smestad,C., Sorensen,P.S., Sondergaard,H.B.,
            Stankovich,J., Strange,R.C., Sulonen,A.M., Sundqvist,E.,
            Syvanen,A.C., Taddeo,F., Taylor,B., Blackwell,J.M., Tienari,P.,
            Bramon,E., Tourbah,A., Brown,M.A., Tronczynska,E., Casas,J.P.,
            Tubridy,N., Corvin,A., Vickery,J., Jankowski,J., Villoslada,P.,
            Markus,H.S., Wang,K., Mathew,C.G., Wason,J., Palmer,C.N.,
            Wichmann,H.E., Plomin,R., Willoughby,E., Rautanen,A.,
            Winkelmann,J., Wittig,M., Trembath,R.C., Yaouanq,J.,
            Viswanathan,A.C., Zhang,H., Wood,N.W., Zuvich,R., Deloukas,P.,
            Langford,C., Duncanson,A., Oksenberg,J.R., Pericak-Vance,M.A.,
            Haines,J.L., Olsson,T., Hillert,J., Ivinson,A.J., De Jager,P.L.,
            Peltonen,L., Stewart,G.J., Hafler,D.A., Hauser,S.L., McVean,G.,
            Donnelly,P. and Compston,A.
  CONSRTM   International Multiple Sclerosis Genetics Consortium; Wellcome
            Trust Case Control Consortium 2
  TITLE     Genetic risk and a primary role for cell-mediated immune mechanisms
            in multiple sclerosis
  JOURNAL   Nature 476 (7359), 214-219 (2011)
   PUBMED   21833088
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 7437)
  AUTHORS   McDonough,C.W., Bostrom,M.A., Lu,L., Hicks,P.J., Langefeld,C.D.,
            Divers,J., Mychaleckyj,J.C., Freedman,B.I. and Bowden,D.W.
  TITLE     Genetic analysis of diabetic nephropathy on chromosome 18 in
            African Americans: linkage analysis and dense SNP mapping
  JOURNAL   Hum Genet 126 (6), 805-817 (2009)
   PUBMED   19690890
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   10 (bases 1 to 7437)
  AUTHORS   Ryazanov,A.G., Pavur,K.S. and Dorovkov,M.V.
  TITLE     Alpha-kinases: a new class of protein kinases with a novel
            catalytic domain
  JOURNAL   Curr Biol 9 (2), R43-R45 (1999)
   PUBMED   10021370
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC104971.5 and AC105105.10.
            
            On Nov 23, 2018 this sequence version replaced NM_052947.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX647796.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000361673.4/ ENSP00000354991.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-134               AC104971.5         68389-68522
            135-328             AC104971.5         68523-68716
            329-457             AC104971.5         85663-85791
            458-575             AC104971.5         90041-90158
            576-2310            AC104971.5         116932-118666
            2311-5701           AC105105.10        12117-15507
            5702-5849           AC105105.10        21103-21250
            5850-5977           AC105105.10        26279-26406
            5978-6013           AC105105.10        26500-26535
            6014-6288           AC105105.10        33159-33433
            6289-6377           AC105105.10        35260-35348
            6378-6595           AC105105.10        46193-46410
            6596-6644           AC105105.10        52244-52292
            6645-7437           AC105105.10        68302-69094
FEATURES             Location/Qualifiers
     source          1..7437
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q21.31-q21.32"
     gene            1..7437
                     /gene="ALPK2"
                     /gene_synonym="HAK"
                     /note="alpha kinase 2"
                     /db_xref="GeneID:115701"
                     /db_xref="HGNC:HGNC:20565"
                     /db_xref="MIM:619965"
     exon            1..328
                     /gene="ALPK2"
                     /gene_synonym="HAK"
                     /inference="alignment:Splign:2.1.0"
     exon            329..457
                     /gene="ALPK2"
                     /gene_synonym="HAK"
                     /inference="alignment:Splign:2.1.0"
     CDS             349..6861
                     /gene="ALPK2"
                     /gene_synonym="HAK"
                     /EC_number="2.7.11.1"
                     /note="heart alpha-kinase; heart alpha-protein kinase"
                     /codon_start=1
                     /product="alpha-protein kinase 2"
                     /protein_id="NP_443179.3"
                     /db_xref="CCDS:CCDS11966.2"
                     /db_xref="GeneID:115701"
                     /db_xref="HGNC:HGNC:20565"
                     /db_xref="MIM:619965"
                     /translation="MKDSEGPQRPPLCFLSTLLSQKVPEKSDAVLRCIISGQPKPEVT
                     WYKNGQAIDGSGIISNYEFFENQYIHVLHLSCCTKNDAAVYQISAKNSFGMICCSASV
                     EVECSSENPQLSPNLEDDRDRGWKHETGTHEEERANQIDEKEHPYKEEESISPGTPRS
                     ADSSPSKSNHSLSLQSLGNLDISVSSSENPLGVKGTRHTGEAYDPSNTEEIANGLLFL
                     NSSHIYEKQDRCCHKTVHSMASKFTDGDLNNDGPHDEGLRSSQQNPKVQKYISFSLPL
                     SEATAHIYPGDSAVANKQPSPQLSSEDSDSDYELCPEITLTYTEEFSDDDLEYLECSD
                     VMTDYSNAVWQRNLLGTEHVFLLESDDEEMEFGEHCLGGCEHFLSGMGCGSRVSGDAG
                     PMVATAGFCGHHSQPQEVGVRSSRVSKHGPSSPQTGMTLILGPHQDGTSSVTEQGRYK
                     LPTAPEAAENDYPGIQGETRDSHQAREEFASDNLLNMDESVRETEMKLLSGESENSGM
                     SQCWETAADKRVGGKDLWSKRGSRKSARVRQPGMKGNPKKPNANLRESTTEGTLHLCS
                     AKESAEPPLTQSDKRETSHTTAAATGRSSHADARECAISTQAEQEAKTLQTSTDSVSK
                     EGNTNCKGEGMQVNTLFETSQVPDWSDPPQVQVQETVRETISCSQMPAFSEPAGEESP
                     FTGTTTISFSNLGGVHKENASLAQHSEVKPCTCGPQHEEKQDRDGNIPDNFREDLKYE
                     QSISEANDETMSPGVFSRHLPKDARADFREPVAVSVASPEPTDTALTLENVCDEPRDR
                     EAVCAMECFEAGDQGTCFDTIDSLVGRPVDKYSPQEICSVDTELAEGQNKVSDLCSSN
                     DKTLEVFFQTQVSETSVSTCKSSKDGNSVMSPLFTSTFTLNISHTASEGATGENLAKV
                     ENSTYPLASTVHAGQEQPSPSNSGGLDETQLLSSENNPLVQFKEGGDKSPSPSAADTT
                     ATPASYSSIVSFPWEKPTTLTANNECFQATRETEDTSTVTIATEVHPAKYLAVSIPED
                     KHAGGTEERFPRASHEKVSQFPSQVQLDHILSGATIKSTKELLCRAPSVPGVPHHVLQ
                     LPEGEGFCSNSPLQVDNLSGDKSQTVDRADFRSYEENFQERGSETKQGVQQQSLSQQG
                     SLSAPDFQQSLPTTSAAQEERNLVPTAHSPASSREGAGQRSGWGTRVSVVAETAGEED
                     SQALSNVPSLSDILLEESKEYRPGNWEAGNKLKIITLEASASEIWPPRQLTNSESKAS
                     DGGLIIPDKVWAVPDSLKADAVVPELAPSEIAALAHSPEDAESALADSRESHKGEEPT
                     ISVHWRSLSSRGFSQPRLLESSVDPVDEKELSVTDSLSAASETGGKENVNNVSQDQEE
                     KQLKMDHTAFFKKFLTCPKILESSVDPIDEISVIEYTRAGKPEPSETTPQGAREGGQS
                     NDGNMGHEAEIQPAILQVPCLQGTILSENRISRSQEGSMKQEAEQIQPEEAKTAIWQV
                     LQPSEGGERIPSGCSIGQIQESSDGSLGEAEQSKKDKAELISPTSPLSSCLPIMTHAS
                     LGVDTHNSTGQIHDVPENDIVEPRKRQYVFPVSQKRGTIENERGKPLPSSPDLTRFPC
                     TSSPEGNVTDFLISHKMEEPKIEVLQIGETKPPSSSSSSAKTLAFISGERELEKAPKL
                     LQDPCQKGTLGCAKKSREREKSLEARAGKSPGTLTAVTGSEEVKRKPEAPGSGHLAEG
                     VKKKILSRVAALRLKLEEKENIRKNSAFLKKMPKLETSLSHTEEKQDPKKPSCKREGR
                     APVLLKKIQAEMFPEHSGNVKLSCQFAEIHEDSTICWTKDSKSIAQVQRSAGDNSTVS
                     FAIVQASPKDQGLYYCCIKNSYGKVTAEFNLTAEVLKQLSSRQDTKGCEEIEFSQLIF
                     KEDFLHDSYFGGRLRGQIATEELHFGEGVHRKAFRSTVMHGLMPVFKPGHACVLKVHN
                     AIAYGTRNNDELIQRNYKLAAQECYVQNTARYYAKIYAAEAQPLEGFGEVPEIIPIFL
                     IHRPENNIPYATVEEELIGEFVKYSIRDGKEINFLRRESEAGQKCCTFQHWVYQKTSG
                     CLLVTDMQGVGMKLTDVGIATLAKGYKGFKGNCSMTFIDQFKALHQCNKYCKMLGLKS
                     LQNNNQKQKQPSIGKSKVQTNSMTIKKAGPETPGEKKT"
     exon            458..575
                     /gene="ALPK2"
                     /gene_synonym="HAK"
                     /inference="alignment:Splign:2.1.0"
     exon            576..2310
                     /gene="ALPK2"
                     /gene_synonym="HAK"
                     /inference="alignment:Splign:2.1.0"
     exon            2311..5701
                     /gene="ALPK2"
                     /gene_synonym="HAK"
                     /inference="alignment:Splign:2.1.0"
     exon            5702..5849
                     /gene="ALPK2"
                     /gene_synonym="HAK"
                     /inference="alignment:Splign:2.1.0"
     exon            5850..5977
                     /gene="ALPK2"
                     /gene_synonym="HAK"
                     /inference="alignment:Splign:2.1.0"
     exon            5978..6013
                     /gene="ALPK2"
                     /gene_synonym="HAK"
                     /inference="alignment:Splign:2.1.0"
     exon            6014..6288
                     /gene="ALPK2"
                     /gene_synonym="HAK"
                     /inference="alignment:Splign:2.1.0"
     exon            6289..6377
                     /gene="ALPK2"
                     /gene_synonym="HAK"
                     /inference="alignment:Splign:2.1.0"
     exon            6378..6595
                     /gene="ALPK2"
                     /gene_synonym="HAK"
                     /inference="alignment:Splign:2.1.0"
     exon            6596..6644
                     /gene="ALPK2"
                     /gene_synonym="HAK"
                     /inference="alignment:Splign:2.1.0"
     exon            6645..7437
                     /gene="ALPK2"
                     /gene_synonym="HAK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7414..7419
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ALPK2"
                     /gene_synonym="HAK"
                     /note="hexamer: ATTAAA"
     polyA_site      7437
                     /gene="ALPK2"
                     /gene_synonym="HAK"
                     /note="major polyA site"
ORIGIN      
        1 agaaacagcc ctcaagtttc atcacaggga tgctaaaata tattctccag cttcagcaat
       61 gacctagttc actgaagggc ggggaaaaaa tgctcttctt ccagaacttc cccgggcatg
      121 gaatttcccc tcttaggaag agatgaactt tctggaagca gacccatcta tgctttgacc
      181 tgatcatgga agaccctgag ccctataaac tcctttgaat ccctgtcccg ggaacaggga
      241 cacccaatca atgcctcaca cacagcctgg ggaatccaca gctgagccag ccctcctaca
      301 acatttcttg gacaaagagt actgaaaaat ttggtgcggc atgaaaggat gaaagactcc
      361 gaagggcccc agaggccccc gctgtgtttt ttatctacat tgctttccca gaaggttcct
      421 gagaagtcag acgctgtgct tcgctgcata atatctggtc agcccaagcc agaggtaact
      481 tggtataaga atggtcaggc catcgatggg agtggcatta tttccaacta tgaattcttt
      541 gagaatcagt atattcatgt gttacatctc tcttgctgta ccaaaaatga tgctgctgtc
      601 tatcaaatct cggctaaaaa ctcttttgga atgatctgtt gttctgcttc cgttgaggtt
      661 gagtgctcat cagagaaccc acaattgtct cctaacctgg aagatgacag ggacaggggt
      721 tggaaacatg aaacagggac acatgaagaa gaaagggcaa atcagattga tgagaaggaa
      781 catccttata aggaagaaga aagcatctcc ccgggcactc ccaggtcagc tgactcctcc
      841 ccctccaaat ccaaccattc actctccctc cagtcattgg gcaatcttga cattagtgtg
      901 tccagttctg aaaatccttt gggtgttaaa ggaacaaggc acactggaga ggcttatgat
      961 ccaagtaaca cagaagaaat tgcaaatggg ttgctttttc ttaattcaag tcatatttat
     1021 gaaaaacaag acagatgttg ccacaagaca gtgcattcca tggcatcaaa gttcacggat
     1081 ggtgacctga acaatgatgg tcctcatgat gaaggcttac gctctagtca gcaaaatccc
     1141 aaagtacaga aatacattag cttcagcctc ccgctatctg aggcaactgc acacatttac
     1201 ccaggtgaca gtgccgtggc caacaaacaa cccagcccac agctttccag tgaagactct
     1261 gacagtgact atgaactttg cccagagata accctaacct acaccgagga gttttcagat
     1321 gatgacctgg agtatctgga atgttctgat gttatgacgg attactctaa tgcagtttgg
     1381 caaaggaacc tgctggggac tgagcatgtt tttttattag aaagcgatga cgaagagatg
     1441 gaattcggtg agcattgcct gggtgggtgt gagcatttcc tcagtggaat gggttgtggg
     1501 tctcgggtgt cgggtgacgc tgggcctatg gttgccactg ctggcttctg tggtcatcac
     1561 tcacaacccc aagaagttgg ggtgaggagc agcagagtct ccaagcacgg tccctcatcc
     1621 ccacaaacag ggatgactct cattttggga cctcaccagg atggaacgtc ttcagtgaca
     1681 gaacagggga gatataaact ccccactgct cccgaggctg ctgaaaatga ttatccagga
     1741 attcaaggag aaaccagaga cagccaccaa gcaagagagg aatttgccag tgacaatctg
     1801 ctcaacatgg atgaatcagt aagagagaca gagatgaagc tcttgtctgg tgagtcagaa
     1861 aactcaggga tgagccagtg ttgggagacg gcagctgaca agagagtggg gggaaaggac
     1921 ttatggagca agaggggttc aaggaaatct gccagggtga ggcagccggg aatgaaggga
     1981 aatcccaaga agccgaatgc caacctgaga gaaagtacaa cagaaggtac ccttcatctc
     2041 tgctctgcca aagaatctgc tgagccccca ctaacccaga gtgataaaag agagacttct
     2101 cacaccacag cagcagcgac tggtcggagt tcccatgctg atgcaagaga atgtgctatt
     2161 tcaacccagg cagagcaaga agcaaaaacc cttcaaactt caacagactc agtctccaaa
     2221 gaaggcaaca caaattgcaa gggagaaggc atgcaagtta atactctatt tgaaacaagc
     2281 caggttccag actggagtga tcctcctcag gtacaagttc aggaaacagt cagagagaca
     2341 atctcttgca gccagatgcc agctttctca gagcctgctg gggaggagtc cccattcact
     2401 gggaccacaa caatttcctt ctcaaactta ggaggggtcc acaaggaaaa tgcatcatta
     2461 gctcaacact cggaggtcaa accctgtacc tgtggtccac agcatgaaga aaaacaagac
     2521 agagatggca acatacctga caatttcagg gaagacctaa aatatgagca gagcatctca
     2581 gaagccaatg atgagactat gtccccaggt gtgttctcaa ggcatctccc caaggatgct
     2641 cgtgctgact tcagggagcc tgtggctgtc tctgttgctt cccctgaacc cacagatact
     2701 gccctcaccc tggaaaatgt gtgtgatgag ccaagggaca gagaagcagt gtgtgcaatg
     2761 gagtgttttg aggctggtga ccaaggaacg tgttttgata ccatagattc tcttgttggg
     2821 agaccagttg ataaatattc gcctcaagaa atttgctctg tagatacgga actggcagaa
     2881 ggtcaaaaca aagtatctga tttatgttct tctaatgaca agacactgga agtctttttt
     2941 cagacacaag tgtctgagac ttcagtgtct acgtgcaaaa gcagcaagga cggcaactca
     3001 gtcatgtccc ctctttttac cagtactttc accttgaaca tttcacacac agctagtgaa
     3061 ggtgccacag gagaaaatct agccaaggtg gagaattcca cctacccact ggcctccaca
     3121 gtacatgctg gccaggagca gccaagcccc agcaactcag gagggcttga tgaaacacag
     3181 ctcctttctt ctgagaacaa tcctttagtg caatttaaag aaggaggtga caagagcccc
     3241 agtcctagtg ccgcagacac cacagccaca ccagccagtt atagttcaat tgtgagtttt
     3301 ccttgggaga agccaacaac attaactgct aataatgagt gctttcaagc gaccagggag
     3361 actgaggaca catcaactgt taccattgcc accgaagtcc acccagccaa ataccttgct
     3421 gtgtcaattc ctgaggacaa gcatgcaggt ggcactgagg agaggttccc tcgtgcatcc
     3481 catgaaaagg tttcccaatt tccttcccaa gtgcagttgg atcatatttt aagtggtgct
     3541 accatcaaat ctacaaaaga gctactttgc agggcaccca gtgtgccagg agtcccacac
     3601 catgtcctgc agctcccaga gggagagggt ttctgcagta attcccctct tcaggttgat
     3661 aacctgtctg gagataagag ccagactgtg gacagagcag actttaggag ctatgaagag
     3721 aatttccaag aaagaggaag tgaaacaaag cagggggtcc agcagcagag cctgtcccag
     3781 cagggttctc tttctgcacc tgatttccaa caaagtttgc ctacgacatc tgctgcacaa
     3841 gaggaaagaa acttggtgcc cacggcccac tcacccgcaa gctctaggga aggagcaggg
     3901 cagcgctcag gttgggggac gagggtctcc gtggtggctg aaactgctgg ggaagaagac
     3961 agtcaggctc tgagcaacgt tccatctctc tctgatatcc ttttggaaga gtctaaagaa
     4021 tatagacctg gaaattggga ggcaggcaac aagctgaaga ttataactct agaggcttcc
     4081 gcttctgaaa tctggccacc acgacaactg acaaattctg agagcaaggc atcagacggt
     4141 ggtctcataa ttcctgacaa ggtctgggct gtacctgata gtctaaaggc agatgctgtt
     4201 gtgcctgaat tggccccctc tgaaatagca gcattggctc acagtccaga ggatgctgag
     4261 tcagcccttg ctgatagcag agaaagccat aaaggcgaag agcccaccat cagtgtacat
     4321 tggagaagtc tttcttcccg gggtttcagc caacccagac tcctggagtc atccgtggac
     4381 cctgtagatg aaaaggagtt atctgtcaca gattcactgt cagcggcttc tgaaactgga
     4441 gggaaggaaa atgttaacaa tgtgagtcaa gaccaggagg aaaaacaact caagatggat
     4501 cacactgcct tctttaaaaa gtttctgacc tgccctaaaa tcctagagtc ctctgtagat
     4561 cccattgatg agataagtgt gatagagtac accagggctg gaaaaccaga gccctctgaa
     4621 accacaccac agggcgccag agaagggggt caatcaaatg acggaaacat gggccacgaa
     4681 gcggaaatcc agccggccat tttgcaagtt ccatgtctcc agggaaccat tctgagtgaa
     4741 aatagaatca gcagaagcca agaaggcagt atgaagcagg aggctgaaca aattcaacct
     4801 gaggaggcaa aaactgccat ttggcaagtc ctgcaaccca gcgaaggcgg tgaaagaatt
     4861 ccaagtggat gtagcatagg ccaaatacaa gaaagcagtg atgggagctt aggggaggct
     4921 gagcaaagca aaaaggacaa agcagaattg atttccccca cttcacctct ttctagttgt
     4981 cttccaataa tgactcacgc ttctcttggg gttgacacgc acaactccac aggccaaatt
     5041 catgacgtcc ctgaaaatga catagttgag cccagaaagc gtcagtatgt gtttcctgtt
     5101 tcacagaaaa ggggaactat tgagaatgag cgtgggaaac ctttgccctc ttctcctgat
     5161 cttaccaggt tcccttgtac ttcatctcct gaaggaaatg tcacagactt tttgataagc
     5221 cacaaaatgg aggaacctaa aatagaggtg cttcaaattg gggaaaccaa acccccaagc
     5281 tcatctagct cctcagcgaa gaccttggca tttatttcag gagaacgtga gttagagaaa
     5341 gcccctaaat tactgcagga tccatgtcaa aagggcaccc tgggctgtgc gaaaaagtcc
     5401 agggagagag agaagtccct ggaagcccga gcaggcaaat cgccagggac cctcacagca
     5461 gtgacggggt cagaggaggt caagaggaag ccagaagccc caggcagtgg acatttagct
     5521 gagggagtaa agaagaaaat tttgtccagg gtggcagcac tgaggctgaa actggaagaa
     5581 aaggaaaata tcagaaagaa ctcagccttt cttaaaaaga tgcccaaact cgaaacatca
     5641 ttatcacaca cagaagagaa acaagaccca aaaaagccat cttgcaaaag agaaggaaga
     5701 gctccagtat tactgaaaaa gatccaagct gagatgttcc ctgaacactc tggaaatgta
     5761 aaattaagct gccaatttgc agaaattcat gaagattcta ctatctgctg gacaaaagat
     5821 tcaaagtcca tagcccaagt gcagagaagt gcaggggaca actccactgt ttcctttgcc
     5881 atcgtgcaag ccagtccgaa ggaccaggga ctctattact gctgcatcaa gaacagctac
     5941 ggaaaagtga ctgctgaatt taacctcaca gctgaagttc tcaaacagct gtcaagtcgc
     6001 caggatacta aaggatgtga agagattgaa ttcagccaac tcatcttcaa agaagacttc
     6061 ctccatgaca gctactttgg gggccgcctg cgtggtcaga tcgccacgga ggagctgcac
     6121 tttggagaag gggttcaccg caaagccttc cgcagcacag tgatgcacgg cctcatgcct
     6181 gtcttcaaac ctggccatgc ctgtgtgctt aaggtgcaca atgccattgc ctatgggacc
     6241 agaaataatg atgagctcat ccaaaggaac tacaaactcg ctgcccagga atgctatgtt
     6301 caaaatactg ccaggtatta tgccaagatc tacgctgctg aagcacagcc tctggaaggc
     6361 tttggagaag tacctgagat cattcctatt tttcttatcc atcggcctga gaacaatatc
     6421 ccgtatgcta cagtggagga ggagctgatt ggagaatttg tgaagtattc catcagggat
     6481 gggaaagaaa taaacttctt gagaagagaa tcagaagctg gtcagaaatg ttgcaccttc
     6541 cagcactggg tgtaccagaa aacaagtggc tgcctcctgg tgacggacat gcaaggtgta
     6601 ggaatgaagc taactgacgt tggcatagca acgctggcta aagggtacaa gggatttaaa
     6661 ggcaactgtt ccatgacctt cattgatcag tttaaagcac tacaccagtg taacaagtat
     6721 tgcaaaatgc tgggactgaa atcccttcaa aacaacaacc agaaacagaa gcagccgagc
     6781 attgggaaaa gcaaagttca aacaaactct atgacaataa agaaggcagg gcctgagacc
     6841 ccaggcgaaa agaaaaccta acgtccctgg gtaacctaat ggccactggc tagcagcaca
     6901 caatctcgcc agggaaaatc tgaggccaca caggagagaa tatacagcct gcagagagtg
     6961 cgtggcaatc cttactccca gccgactgtg cgccaagatg cttctaaacc catcacctgc
     7021 tgtcttcact caaatgattt cagaacagga tttgcgacca ggtttatggg gagattgaat
     7081 caacgattgg tctcaaagac agtccattct ttatatacat gtttagcatt tttaccaacc
     7141 tcacatcatg tgtatatttg tgtatttgca catggttgtg ctgtcgagga cctggtgctg
     7201 agaagagtct gttcacagcc aaaattcttc ccactgtcat tcctaacctg ggatttctag
     7261 acacatcctg ctgtgatgta aacagaaatc acgaattcgc tcactggatc aagttgttcc
     7321 actggtgtct aatacgctat tgttgccgga ggtgggttct gtgacgtgaa gccatttccc
     7381 atcattcaac agccagttac aattttctgt ttaattaaat tcatatttaa acaaaaa
//
