LOCUS       NM_031313               2492 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens alkaline phosphatase, germ cell (ALPG), mRNA.
ACCESSION   NM_031313
VERSION     NM_031313.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Su,Y., Zhang,X., Bidlingmaier,S., Behrens,C.R., Lee,N.K. and Liu,B.
  TITLE     ALPPL2 Is a Highly Specific and Targetable Tumor Cell Surface
            Antigen
  JOURNAL   Cancer Res 80 (20), 4552-4564 (2020)
   PUBMED   32868383
  REMARK    GeneRIF: ALPPL2 Is a Highly Specific and Targetable Tumor Cell
            Surface Antigen.
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Bi,Y., Tu,Z., Zhang,Y., Yang,P., Guo,M., Zhu,X., Zhao,C., Zhou,J.,
            Wang,H., Wang,Y. and Gao,S.
  TITLE     Identification of ALPPL2 as a Naive Pluripotent State-Specific
            Surface Protein Essential for Human Naive Pluripotency Regulation
  JOURNAL   Cell Rep 30 (11), 3917-3931 (2020)
   PUBMED   32187559
  REMARK    GeneRIF: Identification of ALPPL2 as a Naive Pluripotent
            State-Specific Surface Protein Essential for Human Naive
            Pluripotency Regulation.
REFERENCE   3  (bases 1 to 2492)
  AUTHORS   Dudenkov,T.M., Liu,D., Cairns,J., Devarajan,S., Zhuang,Y.,
            Ingle,J.N., Buzdar,A.U., Robson,M.E., Kubo,M., Batzler,A.,
            Barman,P., Jenkins,G.D., Carlson,E.E., Goetz,M.P., Northfelt,D.W.,
            Moreno-Aspitia,A., Desta,Z., Reid,J.M., Kalari,K.R., Wang,L. and
            Weinshilboum,R.M.
  TITLE     Anastrozole Aromatase Inhibitor Plasma Drug Concentration
            Genome-Wide Association Study: Functional Epistatic Interaction
            Between SLC38A7 and ALPPL2
  JOURNAL   Clin Pharmacol Ther 106 (1), 219-227 (2019)
   PUBMED   30648747
  REMARK    GeneRIF: GWAS identified a novel gene encoding an anastrozole
            transporter, SLC38A7, as well as epistatic interaction between SNPs
            in that gene and SNPs near ALPPL2 that influenced both the
            expression of the transporter and anastrozole plasma concentrations
REFERENCE   4  (bases 1 to 2492)
  AUTHORS   Liu,S., Mao,Q., Xue,W., Zhang,X., Qi,Y., Wang,Y., Chen,P. and
            Zhou,Q.
  TITLE     High expression of ALPPL2 is associated with poor prognosis in
            gastric cancer
  JOURNAL   Hum Pathol 86, 49-56 (2019)
   PUBMED   30496798
  REMARK    GeneRIF: Our results indicated that the expression of ALPPL2 was
            significantly increased in gastric adenocarcinoma and was an
            independent factor that could provide reliable prognostic
            information on gastric adenocarcinoma patients
REFERENCE   5  (bases 1 to 2492)
  AUTHORS   Dua,P., Kang,H.S., Hong,S.M., Tsao,M.S., Kim,S. and Lee,D.K.
  TITLE     Alkaline phosphatase ALPPL-2 is a novel pancreatic
            carcinoma-associated protein
  JOURNAL   Cancer Res 73 (6), 1934-1945 (2013)
   PUBMED   23467613
  REMARK    GeneRIF: aptamer-mediated identification of ALPPL-2 on the cell
            surface and cell secretions of pancreatic cancer cells supports its
            potential use in the serum- and membrane-based diagnosis of PDAC
REFERENCE   6  (bases 1 to 2492)
  AUTHORS   Lewis-Jones,D.I., Johnson,P.M., Desmond,A.D. and McLaughlin,P.J.
  TITLE     Germ cell alkaline phosphatase in human seminal plasma following
            vasectomy
  JOURNAL   Br J Urol 69 (4), 418-420 (1992)
   PUBMED   1581814
REFERENCE   7  (bases 1 to 2492)
  AUTHORS   Lowe,M.E.
  TITLE     Site-specific mutations in the COOH-terminus of placental alkaline
            phosphatase: a single amino acid change converts a
            phosphatidylinositol-glycan-anchored protein to a secreted protein
  JOURNAL   J Cell Biol 116 (3), 799-807 (1992)
   PUBMED   1730777
REFERENCE   8  (bases 1 to 2492)
  AUTHORS   Hoylaerts,M.F. and Millan,J.L.
  TITLE     Site-directed mutagenesis and epitope-mapped monoclonal antibodies
            define a catalytically important conformational difference between
            human placental and germ cell alkaline phosphatase
  JOURNAL   Eur J Biochem 202 (2), 605-616 (1991)
   PUBMED   1722150
REFERENCE   9  (bases 1 to 2492)
  AUTHORS   Watanabe,T., Wada,N., Kim,E.E., Wyckoff,H.W. and Chou,J.Y.
  TITLE     Mutation of a single amino acid converts germ cell alkaline
            phosphatase to placental alkaline phosphatase
  JOURNAL   J Biol Chem 266 (31), 21174-21178 (1991)
   PUBMED   1939159
REFERENCE   10 (bases 1 to 2492)
  AUTHORS   Pan,C.J., Sartwell,A.D. and Chou,J.Y.
  TITLE     Transcriptional regulation and the effects of sodium butyrate and
            glycosylation on catalytic activity of human germ cell alkaline
            phosphatase
  JOURNAL   Cancer Res 51 (8), 2058-2062 (1991)
   PUBMED   2009524
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB194270.1, BC014139.2,
            J04948.1 and AI982769.1.
            
            On Nov 23, 2018 this sequence version replaced NM_031313.2.
            
            Summary: There are at least four distinct but related alkaline
            phosphatases: intestinal, placental, placental-like, and
            liver/bone/kidney (tissue non-specific). The product of this gene
            is a membrane bound glycosylated enzyme, localized to testis,
            thymus and certain germ cell tumors, that is closely related to
            both the placental and intestinal forms of alkaline phosphatase.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK293017.1, J04948.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2158569, SAMEA2161674
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000295453.8/ ENSP00000295453.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                DB194270.1         3-15
            14-829              BC014139.2         1-816
            830-1188            J04948.1           831-1189
            1189-1337           BC014139.2         1176-1324
            1338-1667           J04948.1           1339-1668
            1668-2487           BC014139.2         1655-2474
            2488-2492           AI982769.1         6-10                c
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q37.1"
     gene            1..2492
                     /gene="ALPG"
                     /gene_synonym="ALPPL; ALPPL2; GCAP"
                     /note="alkaline phosphatase, germ cell"
                     /db_xref="GeneID:251"
                     /db_xref="HGNC:HGNC:441"
                     /db_xref="MIM:171810"
     exon            1..118
                     /gene="ALPG"
                     /gene_synonym="ALPPL; ALPPL2; GCAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             52..1650
                     /gene="ALPG"
                     /gene_synonym="ALPPL; ALPPL2; GCAP"
                     /EC_number="3.1.3.1"
                     /note="testicular and thymus alkaline phosphatase; Nagao
                     isozyme; germ cell alkaline phosphatase; placental-like
                     alkaline phosphatase; ALP-1; alkaline phosphatase Nagao
                     isozyme; alkaline phosphatase, placental-like; alkaline
                     phosphatase, placental like 2; alkaline phosphatase, germ
                     cell type"
                     /codon_start=1
                     /product="alkaline phosphatase, germ cell type
                     preproprotein"
                     /protein_id="NP_112603.2"
                     /db_xref="CCDS:CCDS2491.1"
                     /db_xref="GeneID:251"
                     /db_xref="HGNC:HGNC:441"
                     /db_xref="MIM:171810"
                     /translation="MQGPWVLLLLGLRLQLSLGIIPVEEENPDFWNRQAAEALGAAKK
                     LQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPETFLAMDRFPYVALSKTY
                     SVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGK
                     SVGVVTTTRVQHASPAGAYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVI
                     LGGGRKYMFPMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKHQGARYVWNRTELLQAS
                     LDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALLLLSRNPRGFFLFVEGGRI
                     DHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSS
                     IFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDGET
                     HAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPRAGTTDAAHPG
                     PSVVPALLPLLAGTLLLLGTATAP"
     exon            119..235
                     /gene="ALPG"
                     /gene_synonym="ALPPL; ALPPL2; GCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            236..351
                     /gene="ALPG"
                     /gene_synonym="ALPPL; ALPPL2; GCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            352..526
                     /gene="ALPG"
                     /gene_synonym="ALPPL; ALPPL2; GCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            527..699
                     /gene="ALPG"
                     /gene_synonym="ALPPL; ALPPL2; GCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            700..834
                     /gene="ALPG"
                     /gene_synonym="ALPPL; ALPPL2; GCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            835..907
                     /gene="ALPG"
                     /gene_synonym="ALPPL; ALPPL2; GCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            908..1042
                     /gene="ALPG"
                     /gene_synonym="ALPPL; ALPPL2; GCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1234
                     /gene="ALPG"
                     /gene_synonym="ALPPL; ALPPL2; GCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..1351
                     /gene="ALPG"
                     /gene_synonym="ALPPL; ALPPL2; GCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1352..2492
                     /gene="ALPG"
                     /gene_synonym="ALPPL; ALPPL2; GCAP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2466..2471
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ALPG"
                     /gene_synonym="ALPPL; ALPPL2; GCAP"
                     /note="hexamer: AATAAA"
     polyA_site      2492
                     /gene="ALPG"
                     /gene_synonym="ALPPL; ALPPL2; GCAP"
                     /note="major polyA site"
ORIGIN      
        1 atactccata cctgggattt ccgcctcgcc gctctccgac tgcttccaga catgcagggg
       61 ccctgggtgc tgctcctgct gggcctgagg ctacagctct ccctgggcat catcccagtt
      121 gaggaggaga acccggactt ctggaaccgc caggcagccg aggccctggg tgccgccaag
      181 aagctgcagc ctgcacagac agccgccaag aacctcatca tcttcctggg tgacgggatg
      241 ggggtgtcta cggtgacagc tgccaggatc ctaaaagggc agaagaagga caaactgggg
      301 cctgagacct tcctggccat ggaccgcttc ccgtacgtgg ctctgtccaa gacatacagt
      361 gtagacaagc atgtgccaga cagtggagcc acagccacgg cctacctgtg cggggtcaag
      421 ggcaacttcc agaccattgg cttgagtgca gccgcccgct ttaaccagtg caacacgaca
      481 cgcggcaacg aggtcatctc cgtgatgaat cgggccaaga aagcaggaaa gtcagtggga
      541 gtggtaacca ccacacgggt gcagcatgcc tcgccagccg gcgcctacgc ccacacggtg
      601 aaccgcaact ggtactcgga tgccgacgtg cctgcctcgg cccgccagga ggggtgccag
      661 gacatcgcca cgcagctcat ctccaacatg gacattgatg tgatcctagg tggaggccga
      721 aagtacatgt ttcccatggg gaccccagac cctgagtacc cagatgacta cagccaaggt
      781 gggaccaggc tggacgggaa gaatctggtg caggaatggc tggcgaagca ccagggtgcc
      841 cggtacgtgt ggaaccgcac tgagctcctg caggcttccc tggacccgtc tgtgacccat
      901 ctcatgggtc tctttgagcc tggagacatg aaatacgaga tccaccgaga ctccacactg
      961 gacccctccc tgatggagat gacagaggct gccctgctcc tgctgagcag gaacccccgc
     1021 ggcttcttcc tcttcgtgga gggtggtcgc atcgaccatg gtcatcatga aagcagggct
     1081 taccgggcac tgactgagac gatcatgttc gacgacgcca ttgagagggc gggccagctc
     1141 accagcgagg aggacacgct gagcctcgtc actgccgacc actcccacgt cttctccttc
     1201 ggaggctacc ccctgcgagg gagctccatc ttcgggctgg cccctggcaa ggcccgggac
     1261 aggaaggcct acacggtcct cctatacgga aacggtccag gctatgtgct caaggacggc
     1321 gcccggccgg atgttacgga gagcgagagc gggagccccg agtatcggca gcagtcagca
     1381 gtgcccctgg acggagagac ccacgcaggc gaggacgtgg cggtgttcgc gcgcggcccg
     1441 caggcgcacc tggttcacgg cgtgcaggag cagaccttca tagcgcacgt catggccttc
     1501 gccgcctgcc tggagcccta caccgcctgc gacctggcgc cccgcgccgg caccaccgac
     1561 gccgcgcacc cggggccgtc cgtggtcccc gcgttgcttc ctctgctggc agggaccttg
     1621 ctgctgctgg ggacggccac tgctccctga gtgtcccgtc cctggggctc ctgcttcccc
     1681 atcccggagt tcccctgctc cccacctcca gtcgtcctgc cggacctcca cctggagctg
     1741 tcacccccgg agtcgccaca cagacgtcct gccatggaac cttcccctcc cggtgcaccc
     1801 tggggaccga gcccttgaca ccacgccctt tgctttatct tgctcttgaa attttggccc
     1861 caactccagg gactggggat ttgtgcctgg cagctgcctg catttcagga aaagaggagg
     1921 ctcagaccat ccagcccccg cccatatcct gaggtggatc aggcaggctc tctccccggg
     1981 gacatgaggc acccatacct aggaccccct gcgccttttt tagcttcagt catggcagca
     2041 cctgagggac acaaggactt gggtgcatca ggacgccttg gagaagcgtg gcttcctgcc
     2101 accctgcaac ccaccctccc agccaaggag gctgctgtgg tggggatccc caggggggct
     2161 ttgacacagt cctctgctgt ccctccactg ggctaattct acacccctgt gcccctccta
     2221 ggggcccatg agtcagagag gcttgcccca agtcacagcc actcagatgt tcgacgcccc
     2281 ctaaggtcca ttccagcacc cacctgagtt ccgaggagca cctgggaagc tctgggtgca
     2341 ggatagcagt ccagagtcca tggccccgcc taggccatct gggtgctggg catggatttc
     2401 tcagcaagga agactcatta ccttccctcc ctgggcctcc attcttctgg gaaacacaaa
     2461 gcaataataa aaggaagtgt tagacaatgt aa
//
