LOCUS       NM_000698               2519 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens arachidonate 5-lipoxygenase (ALOX5), transcript
            variant 1, mRNA.
ACCESSION   NM_000698 XM_937295
VERSION     NM_000698.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Kahnt,A.S., Hafner,A.K. and Steinhilber,D.
  TITLE     The role of human 5-Lipoxygenase (5-LO) in carcinogenesis - a
            question of canonical and non-canonical functions
  JOURNAL   Oncogene 43 (18), 1319-1327 (2024)
   PUBMED   38575760
  REMARK    GeneRIF: The role of human 5-Lipoxygenase (5-LO) in carcinogenesis
            - a question of canonical and non-canonical functions.
            Review article
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Cai,H., Zhang,J., Xu,H., Sun,W., Wu,W., Dong,C., Zhou,P., Xue,C.,
            Nan,Y., Ni,Y., Wu,X., Gu,Z., Chen,M. and Wang,Y.
  TITLE     ALOX5 drives the pyroptosis of CD4+ T cells and tissue inflammation
            in rheumatoid arthritis
  JOURNAL   Sci Signal 17 (825), eadh1178 (2024)
   PUBMED   38412254
  REMARK    GeneRIF: ALOX5 drives the pyroptosis of CD4[+] T cells and tissue
            inflammation in rheumatoid arthritis.
REFERENCE   3  (bases 1 to 2519)
  AUTHORS   Han,L., Chen,Y., Huang,N., Zhou,X., Lv,Y., Li,H., Chai,D., Zheng,J.
            and Wang,G.
  TITLE     Cancer-educated neutrophils promote lung cancer progression via
            PARP-1-ALOX5-mediated MMP-9 expression
  JOURNAL   Cancer Biol Med 21 (2), 175-192 (2024)
   PUBMED   38172525
  REMARK    GeneRIF: Cancer-educated neutrophils promote lung cancer
            progression via PARP-1-ALOX5-mediated MMP-9 expression.
REFERENCE   4  (bases 1 to 2519)
  AUTHORS   Huang,J., Xie,Y., Chen,B., Xia,Y., Jiang,Y., Sun,Z. and Liu,Y.
  TITLE     GPR146 regulates pulmonary vascular remodeling by promoting
            pulmonary artery smooth muscle cell proliferation through
            5-lipoxygenase
  JOURNAL   Eur J Pharmacol 961, 176123 (2023)
   PUBMED   37926274
  REMARK    GeneRIF: GPR146 regulates pulmonary vascular remodeling by
            promoting pulmonary artery smooth muscle cell proliferation through
            5-lipoxygenase.
REFERENCE   5  (bases 1 to 2519)
  AUTHORS   Li,X., Chen,G., Wang,F., Guo,X., Zhang,R., Liu,P., Dong,L., Yu,W.,
            Wang,H., Wang,H. and Yu,J.
  TITLE     Oncogenic PIK3CA recruits myeloid-derived suppressor cells to shape
            the immunosuppressive tumour microenvironment in luminal breast
            cancer through the 5-lipoxygenase-dependent arachidonic acid
            pathway
  JOURNAL   Clin Transl Med 13 (11), e1483 (2023)
   PUBMED   37965796
  REMARK    GeneRIF: Oncogenic PIK3CA recruits myeloid-derived suppressor cells
            to shape the immunosuppressive tumour microenvironment in luminal
            breast cancer through the 5-lipoxygenase-dependent arachidonic acid
            pathway.
REFERENCE   6  (bases 1 to 2519)
  AUTHORS   Ishii,S., Noguchi,M., Miyano,M., Matsumoto,T. and Noma,M.
  TITLE     Mutagenesis studies on the amino acid residues involved in the
            iron-binding and the activity of human 5-lipoxygenase
  JOURNAL   Biochem Biophys Res Commun 182 (3), 1482-1490 (1992)
   PUBMED   1540191
  REMARK    Erratum:[Biochem Biophys Res Commun. 1992 Apr 30;184(2):1133-4.
            doi: 10.1016/0006-291x(92)90710-3.. PMID: 1575732]
REFERENCE   7  (bases 1 to 2519)
  AUTHORS   Nguyen,T., Falgueyret,J.P., Abramovitz,M. and Riendeau,D.
  TITLE     Evaluation of the role of conserved His and Met residues among
            lipoxygenases by site-directed mutagenesis of recombinant human
            5-lipoxygenase
  JOURNAL   J Biol Chem 266 (32), 22057-22062 (1991)
   PUBMED   1939225
REFERENCE   8  (bases 1 to 2519)
  AUTHORS   Hoshiko,S., Radmark,O. and Samuelsson,B.
  TITLE     Characterization of the human 5-lipoxygenase gene promoter
  JOURNAL   Proc Natl Acad Sci U S A 87 (23), 9073-9077 (1990)
   PUBMED   2251250
REFERENCE   9  (bases 1 to 2519)
  AUTHORS   Funk,C.D., Hoshiko,S., Matsumoto,T., Rdmark,O. and Samuelsson,B.
  TITLE     Characterization of the human 5-lipoxygenase gene
  JOURNAL   Proc Natl Acad Sci U S A 86 (8), 2587-2591 (1989)
   PUBMED   2565035
REFERENCE   10 (bases 1 to 2519)
  AUTHORS   Matsumoto,T., Funk,C.D., Radmark,O., Hoog,J.O., Jornvall,H. and
            Samuelsson,B.
  TITLE     Molecular cloning and amino acid sequence of human 5-lipoxygenase
  JOURNAL   Proc Natl Acad Sci U S A 85 (1), 26-30 (1988)
   PUBMED   2829172
  REMARK    Erratum:[Proc Natl Acad Sci U S A 1988 May;85(10):3406]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC132677.1 and AW403395.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_000698.4.
            
            Summary: This gene encodes a member of the lipoxygenase gene family
            and plays a dual role in the synthesis of leukotrienes from
            arachidonic acid. The encoded protein, which is expressed
            specifically in bone marrow-derived cells, catalyzes the conversion
            of arachidonic acid to
            5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and
            further to the allylic epoxide
            5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene
            A4). Leukotrienes are important mediators of a number of
            inflammatory and allergic conditions. Mutations in the promoter
            region of this gene lead to a diminished response to
            antileukotriene drugs used in the treatment of asthma and may also
            be associated with atherosclerosis and several cancers.
            Alternatively spliced transcript variants encoding different
            isoforms have been found for this gene. [provided by RefSeq, Jan
            2012].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.917501.1,
                                           SRR3476690.1169434.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000374391.7/ ENSP00000363512.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2346              BC132677.1         4-2349
            2347-2519           AW403395.1         48-220              c
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q11.21"
     gene            1..2519
                     /gene="ALOX5"
                     /gene_synonym="5-LO; 5-LOX; 5LPG; LOG5"
                     /note="arachidonate 5-lipoxygenase"
                     /db_xref="GeneID:240"
                     /db_xref="HGNC:HGNC:435"
                     /db_xref="MIM:152390"
     exon            1..214
                     /gene="ALOX5"
                     /gene_synonym="5-LO; 5-LOX; 5LPG; LOG5"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..2089
                     /gene="ALOX5"
                     /gene_synonym="5-LO; 5-LOX; 5LPG; LOG5"
                     /EC_number="1.13.11.34"
                     /note="isoform 1 is encoded by transcript variant 1;
                     arachidonic acid 5-lipoxygenase; leukotriene A4 synthase;
                     LOX-5; polyunsaturated fatty acid 5-lipoxygenase"
                     /codon_start=1
                     /product="polyunsaturated fatty acid 5-lipoxygenase
                     isoform 1"
                     /protein_id="NP_000689.1"
                     /db_xref="CCDS:CCDS7212.1"
                     /db_xref="GeneID:240"
                     /db_xref="HGNC:HGNC:435"
                     /db_xref="MIM:152390"
                     /translation="MPSYTVTVATGSQWFAGTDDYIYLSLVGSAGCSEKHLLDKPFYN
                     DFERGAVDSYDVTVDEELGEIQLVRIEKRKYWLNDDWYLKYITLKTPHGDYIEFPCYR
                     WITGDVEVVLRDGRAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKC
                     HKDLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNT
                     ISERVMNHWQEDLMFGYQFLNGCNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQE
                     VQQGNIFIVDFELLDGIDANKTDPCTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGD
                     ENPIFLPSDAKYDWLLAKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHP
                     IFKLLVAHVRFTIAINTKAREQLICECGLFDKANATGGGGHVQMVQRAMKDLTYASLC
                     FPEAIKARGMESKEDIPYYFYRDDGLLVWEAIRTFTAEVVDIYYEGDQVVEEDPELQD
                     FVNDVYVYGMRGRKSSGFPKSVKSREQLSEYLTVVIFTASAQHAAVNFGQYDWCSWIP
                     NAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCWHLGAVWALSQFQENELFLGMYPEE
                     HFIEKPVKEAMARFRKNLEAIVSVIAERNKKKQLPYYYLSPDRIPNSVAI"
     exon            215..413
                     /gene="ALOX5"
                     /gene_synonym="5-LO; 5-LOX; 5LPG; LOG5"
                     /inference="alignment:Splign:2.1.0"
     exon            414..495
                     /gene="ALOX5"
                     /gene_synonym="5-LO; 5-LOX; 5LPG; LOG5"
                     /inference="alignment:Splign:2.1.0"
     exon            496..618
                     /gene="ALOX5"
                     /gene_synonym="5-LO; 5-LOX; 5LPG; LOG5"
                     /inference="alignment:Splign:2.1.0"
     exon            619..725
                     /gene="ALOX5"
                     /gene_synonym="5-LO; 5-LOX; 5LPG; LOG5"
                     /inference="alignment:Splign:2.1.0"
     exon            726..898
                     /gene="ALOX5"
                     /gene_synonym="5-LO; 5-LOX; 5LPG; LOG5"
                     /inference="alignment:Splign:2.1.0"
     exon            899..1045
                     /gene="ALOX5"
                     /gene_synonym="5-LO; 5-LOX; 5LPG; LOG5"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1249
                     /gene="ALOX5"
                     /gene_synonym="5-LO; 5-LOX; 5LPG; LOG5"
                     /inference="alignment:Splign:2.1.0"
     exon            1250..1336
                     /gene="ALOX5"
                     /gene_synonym="5-LO; 5-LOX; 5LPG; LOG5"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..1515
                     /gene="ALOX5"
                     /gene_synonym="5-LO; 5-LOX; 5LPG; LOG5"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..1637
                     /gene="ALOX5"
                     /gene_synonym="5-LO; 5-LOX; 5LPG; LOG5"
                     /inference="alignment:Splign:2.1.0"
     exon            1638..1738
                     /gene="ALOX5"
                     /gene_synonym="5-LO; 5-LOX; 5LPG; LOG5"
                     /inference="alignment:Splign:2.1.0"
     exon            1739..1909
                     /gene="ALOX5"
                     /gene_synonym="5-LO; 5-LOX; 5LPG; LOG5"
                     /inference="alignment:Splign:2.1.0"
     exon            1910..2519
                     /gene="ALOX5"
                     /gene_synonym="5-LO; 5-LOX; 5LPG; LOG5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2499..2504
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ALOX5"
                     /gene_synonym="5-LO; 5-LOX; 5LPG; LOG5"
                     /note="hexamer: AATAAA"
     polyA_site      2519
                     /gene="ALOX5"
                     /gene_synonym="5-LO; 5-LOX; 5LPG; LOG5"
                     /note="major polyA site"
ORIGIN      
        1 agatgcggac acctggaccg ccgcgccgag gctcccggcg ctcgctgctc ccgcggcccg
       61 cgccatgccc tcctacacgg tcaccgtggc cactggcagc cagtggttcg ccggcactga
      121 cgactacatc tacctcagcc tcgtgggctc ggcgggctgc agcgagaagc acctgctgga
      181 caagcccttc tacaacgact tcgagcgtgg cgcggtggat tcatacgacg tgactgtgga
      241 cgaggaactg ggcgagatcc agctggtcag aatcgagaag cgcaagtact ggctgaatga
      301 cgactggtac ctgaagtaca tcacgctgaa gacgccccac ggggactaca tcgagttccc
      361 ctgctaccgc tggatcaccg gcgatgtcga ggttgtcctg agggatggac gcgcaaagtt
      421 ggcccgagat gaccaaattc acattctcaa gcaacaccga cgtaaagaac tggaaacacg
      481 gcaaaaacaa tatcgatgga tggagtggaa ccctggcttc cccttgagca tcgatgccaa
      541 atgccacaag gatttacccc gtgatatcca gtttgatagt gaaaaaggag tggactttgt
      601 tctgaattac tccaaagcga tggagaacct gttcatcaac cgcttcatgc acatgttcca
      661 gtcttcttgg aatgacttcg ccgactttga gaaaatcttt gtcaagatca gcaacactat
      721 ttctgagcgg gtcatgaatc actggcagga agacctgatg tttggctacc agttcctgaa
      781 tggctgcaac cctgtgttga tccggcgctg cacagagctg cccgagaagc tcccggtgac
      841 cacggagatg gtagagtgca gcctggagcg gcagctcagc ttggagcagg aggtccagca
      901 agggaacatt ttcatcgtgg actttgagct gctggatggc atcgatgcca acaaaacaga
      961 cccctgcaca ctccagttcc tggccgctcc catctgcttg ctgtataaga acctggccaa
     1021 caagattgtc cccattgcca tccagctcaa ccaaatcccg ggagatgaga accctatttt
     1081 cctcccttcg gatgcaaaat acgactggct tttggccaaa atctgggtgc gttccagtga
     1141 cttccacgtc caccagacca tcacccacct tctgcgaaca catctggtgt ctgaggtttt
     1201 tggcattgca atgtaccgcc agctgcctgc tgtgcacccc attttcaagc tgctggtggc
     1261 acacgtgaga ttcaccattg caatcaacac caaggcccgt gagcagctca tctgcgagtg
     1321 tggcctcttt gacaaggcca acgccacagg gggcggtggg cacgtgcaga tggtgcagag
     1381 ggccatgaag gacctgacct atgcctccct gtgctttccc gaggccatca aggcccgggg
     1441 catggagagc aaagaagaca tcccctacta cttctaccgg gacgacgggc tcctggtgtg
     1501 ggaagccatc aggacgttca cggccgaggt ggtagacatc tactacgagg gcgaccaggt
     1561 ggtggaggag gacccggagc tgcaggactt cgtgaacgat gtctacgtgt acggcatgcg
     1621 gggccgcaag tcctcaggct tccccaagtc ggtcaagagc cgggagcagc tgtcggagta
     1681 cctgaccgtg gtgatcttca ccgcctccgc ccagcacgcc gcggtcaact tcggccagta
     1741 cgactggtgc tcctggatcc ccaatgcgcc cccaaccatg cgagccccgc caccgactgc
     1801 caagggcgtg gtgaccattg agcagatcgt ggacacgctg cccgaccgcg gccgctcctg
     1861 ctggcatctg ggtgcagtgt gggcgctgag ccagttccag gaaaacgagc tgttcctggg
     1921 catgtaccca gaagagcatt ttatcgagaa gcctgtgaag gaagccatgg cccgattccg
     1981 caagaacctc gaggccattg tcagcgtgat tgctgagcgc aacaagaaga agcagctgcc
     2041 atattactac ttgtccccag accggattcc gaacagtgtg gccatctgag cacactgcca
     2101 gtctcactgt gggaaggcca gctgccccag ccagatggac tccagcctgc ctggcaggct
     2161 gtctggccag gcctcttggc agtcacatct cttcctccga ggccagtacc tttccattta
     2221 ttctttgatc ttcagggaac tgcatagatt gatcaaagtg taaacaccat agggacccat
     2281 tctacacaga gcaggactgc acagcgtcct gtccacaccc agctcagcat ttccacacca
     2341 agcagcaaca gcaaatcacg accactgata gatgtctatt cttgttggag acatgggatg
     2401 attattttct gttctatttg tgcttagtcc aattccttgc acatagtagg tacccaattc
     2461 aattactatt gaatgaatta agaattggtt gccataaaaa taaatcagtt catttaaaa
//
