LOCUS       NM_001378454           12844 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens ALMS1 centrosome and basal body associated protein
            (ALMS1), transcript variant 2, mRNA.
ACCESSION   NM_001378454
VERSION     NM_001378454.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12844)
  AUTHORS   Zhang,S., Gong,S., Li,M., Cai,X., Liu,W., Lou,Y., Ma,Y., Zhang,X.,
            Ren,Q., Zhu,Y., Wu,J., Zhou,L., Li,Y., Zhou,X., Han,X. and Ji,L.
  TITLE     Overburden of rare ALMS1 deleterious variants in Chinese
            early-onset type 2 diabetes with severe insulin resistance
  JOURNAL   Diabetes Metab Res Rev 40 (4), e3788 (2024)
   PUBMED   38546151
  REMARK    GeneRIF: Overburden of rare ALMS1 deleterious variants in Chinese
            early-onset type 2 diabetes with severe insulin resistance.
REFERENCE   2  (bases 1 to 12844)
  AUTHORS   Queiroz,I.C., Carasek,N., Ferreira,L.C.V., Oliveira,L.A.T.,
            Correia,F.M., Elias,T.G.A. and Bahmad,F. Jr.
  TITLE     New variants of ALMS1 gene and familial Alstrom syndrome case
            series
  JOURNAL   Braz J Otorhinolaryngol 90 (3), 101402 (2024)
   PUBMED   38428329
  REMARK    GeneRIF: New variants of ALMS1 gene and familial Alstrom syndrome
            case series.
REFERENCE   3  (bases 1 to 12844)
  AUTHORS   Hu,X., Sun,Y., Wang,S., Zhao,H., Wei,Y., Fu,J., Huang,Y., Wu,W.,
            Li,J., Liu,J., Gong,S., Zhao,Q., Wang,L., Jiang,R., Song,X. and
            Yuan,P.
  TITLE     CircALMS1 Alleviates Pulmonary Microvascular Endothelial Cell
            Dysfunction in Pulmonary Hypertension
  JOURNAL   J Am Heart Assoc 13 (6), e031867 (2024)
   PUBMED   38497483
  REMARK    GeneRIF: CircALMS1 Alleviates Pulmonary Microvascular Endothelial
            Cell Dysfunction in Pulmonary Hypertension.
REFERENCE   4  (bases 1 to 12844)
  AUTHORS   Hearn,T., Renforth,G.L., Spalluto,C., Hanley,N.A., Piper,K.,
            Brickwood,S., White,C., Connolly,V., Taylor,J.F.,
            Russell-Eggitt,I., Bonneau,D., Walker,M. and Wilson,D.I.
  TITLE     Mutation of ALMS1, a large gene with a tandem repeat encoding 47
            amino acids, causes Alstrom syndrome
  JOURNAL   Nat Genet 31 (1), 79-83 (2002)
   PUBMED   11941370
  REMARK    GeneRIF: Mutation of ALMS1, a large gene with a tandem repeat
            encoding 47 amino acids, causes Alstrom syndrome.
REFERENCE   5  (bases 1 to 12844)
  AUTHORS   Collin,G.B., Marshall,J.D., Ikeda,A., So,W.V., Russell-Eggitt,I.,
            Maffei,P., Beck,S., Boerkoel,C.F., Sicolo,N., Martin,M.,
            Nishina,P.M. and Naggert,J.K.
  TITLE     Mutations in ALMS1 cause obesity, type 2 diabetes and neurosensory
            degeneration in Alstrom syndrome
  JOURNAL   Nat Genet 31 (1), 74-78 (2002)
   PUBMED   11941369
  REMARK    GeneRIF: Mutations in ALMS1 cause obesity, type 2 diabetes and
            neurosensory degeneration in Alstrom syndrome.
REFERENCE   6  (bases 1 to 12844)
  AUTHORS   Macari,F., Lautier,C., Girardet,A., Dadoun,F., Darmon,P.,
            Dutour,A., Renard,E., Bouvagnet,P., Claustres,M., Oliver,C. and
            Grigorescu,F.
  TITLE     Refinement of genetic localization of the Alstrom syndrome on
            chromosome 2p12-13 by linkage analysis in a North African family
  JOURNAL   Hum Genet 103 (6), 658-661 (1998)
   PUBMED   9921899
REFERENCE   7  (bases 1 to 12844)
  AUTHORS   Collin,G.B., Marshall,J.D., Cardon,L.R. and Nishina,P.M.
  TITLE     Homozygosity mapping at Alstrom syndrome to chromosome 2p
  JOURNAL   Hum Mol Genet 6 (2), 213-219 (1997)
   PUBMED   9063741
REFERENCE   8  (bases 1 to 12844)
  AUTHORS   Golsteyn,R.M., Mundt,K.E., Fry,A.M. and Nigg,E.A.
  TITLE     Cell cycle regulation of the activity and subcellular localization
            of Plk1, a human protein kinase implicated in mitotic spindle
            function
  JOURNAL   J Cell Biol 129 (6), 1617-1628 (1995)
   PUBMED   7790358
REFERENCE   9  (bases 1 to 12844)
  AUTHORS   Kumaran,N., Pennesi,M.E., Yang,P., Trzupek,K.M., Schlechter,C.,
            Moore,A.T., Weleber,R.G. and Michaelides,M.
  TITLE     Leber Congenital Amaurosis / Early-Onset Severe Retinal Dystrophy
            Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   30285347
REFERENCE   10 (bases 1 to 12844)
  AUTHORS   Paisey,R.B., Steeds,R., Barrett,T., Williams,D., Geberhiwot,T. and
            Gunay-Aygun,M.
  TITLE     Alstrom Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301444
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC074008.5, KF573641.1,
            AC096546.1 and AC092653.3.
            
            Summary: This gene encodes a protein containing a large
            tandem-repeat domain as well as additional low complexity regions.
            The encoded protein functions in microtubule organization,
            particularly in the formation and maintanance of cilia. Mutations
            in this gene cause Alstrom syndrome. There is a pseudogene for this
            gene located adjacent in the same region of chromosome 2.
            Alternative splice variants have been described but their full
            length nature has not been determined. [provided by RefSeq, Apr
            2014].
            
            Transcript Variant: This variant (2) encodes isoform 2. This
            transcript and NM_015120.4 have the same exon combination but
            differ in the length of the encoded proteins.
            
            Sequence Note: NM_001378454.1 and NM_015120.4 have the same exon
            combination but differ in the length of their coding sequence (CDS)
            by 1 aa, caused by a 3-nt insert in NM_015120.4 in a simple repeat
            region, compared to NM_001378454.1. NM_015120.4 is the reference
            transcript on public LRG_741; NM_001378454.1 is the MANE Select
            transcript.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ417593.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000613296.6/ ENSP00000482968.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-357               AC074008.5         55331-55687
            358-483             AC074008.5         78117-78242
            484-679             AC074008.5         88618-88813
            680-797             AC074008.5         92352-92469
            798-1270            AC074008.5         93925-94397
            1271-1371           AC074008.5         95948-96048
            1372-1465           AC074008.5         101693-101786
            1466-1779           KF573641.1         361-674
            1780-7573           AC074008.5         117768-123561
            7574-7707           AC074008.5         124656-124789
            7708-9572           AC096546.1         1689-3553
            9573-9814           AC096546.1         31830-32071
            9815-9940           AC096546.1         46879-47004
            9941-10111          AC096546.1         62322-62492
            10112-10246         AC096546.1         69275-69409
            10247-10417         AC096546.1         71027-71197
            10418-11580         AC096546.1         84317-85479
            11581-11701         AC092653.3         9534-9654
            11702-11905         AC092653.3         10811-11014
            11906-12147         AC092653.3         11328-11569
            12148-12331         AC092653.3         12318-12501
            12332-12395         AC092653.3         13374-13437
            12396-12495         AC092653.3         18608-18707
            12496-12844         AC092653.3         19701-20049
FEATURES             Location/Qualifiers
     source          1..12844
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p13.1"
     gene            1..12844
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /note="ALMS1 centrosome and basal body associated protein"
                     /db_xref="GeneID:7840"
                     /db_xref="HGNC:HGNC:428"
                     /db_xref="MIM:606844"
     exon            1..357
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     CDS             34..12540
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /note="isoform 2 is encoded by transcript variant 2;
                     Alstrom syndrome 1; Alstrom syndrome protein 1;
                     centrosome-associated protein ALMS1"
                     /codon_start=1
                     /product="centrosome-associated protein ALMS1 isoform 2"
                     /protein_id="NP_001365383.1"
                     /db_xref="CCDS:CCDS42697.1"
                     /db_xref="GeneID:7840"
                     /db_xref="HGNC:HGNC:428"
                     /db_xref="MIM:606844"
                     /translation="MEPEDLPWPGELEEEEEEEEEEEEEEEEAAAAAAANVDDVVVVE
                     EVEEEAGRELDSDSHYGPQHLESIDDEEDEEAKAWLQAHPGRILPPLSPPQHRYSEGE
                     RTSLEKIVPLTCHVWQQIVYQGNSRTQISDTNVVCLETTAQRGSGDDQKTESWHCLPQ
                     EMDSSQTLDTSQTRFNVRTEDTEVTDFPSLEEGILTQSENQVKEPNRDLFCSPLLVIQ
                     DSFASPDLPLLTCLTQDQEFAPDSLFHQSELSFAPLRGIPDKSEDTEWSSRPSEVSEA
                     LFQATAEVASDLASSRFSVSQHPLIGSTAVGSQCPFLPSEQGNNEETISSVDELKIPK
                     DCDRYDDLCSYMSWKTRKDTQWPENNLADKDQVSVATSFDITDENIATKRSDHFDAAR
                     SYGQYWTQEDSSKQAETYLTKGLQGKVESDVITLDGLNENAVVCSERVAELQRKPTRE
                     SEYHSSDLRMLRMSPDTVPKAPKHLKAGDTSKGGIAKVTQSNLKSGITTTPVDSDIGS
                     HLSLSLEDLSQLAVSSPLETTTGQHTDTLNQKTLADTHLTEETLKVTAIPEPADQKTA
                     TPTVLSSSHSHRGKPSIFYQQGLPDSHLTEEALKVSAAPGLADQTTGMSTLTSTSYSH
                     REKPGTFYQQELPESNLTEEPLEVSAAPGPVEQKTGIPTVSSTSHSHVEDLLFFYRQT
                     LPDGHLTDQALKVSAVSGPADQKTGTATVLSTPHSHREKPGIFYQQEFADSHQTEETL
                     TKVSATPGPADQKTEIPAVQSSSYSQREKPSILYPQDLADSHLPEEGLKVSAVAGPAD
                     QKTGLPTVPSSAYSHREKLLVFYQQALLDSHLPEEALKVSAVSGPADGKTGTPAVTST
                     SSASSSLGEKPSAFYQQTLPNSHLTEEALKVSIVPGPGDQKTGIPSAPSSFYSHREKP
                     IIFSQQTLPDFLFPEEALKVSAVSVLAAQKTGTPTVSSNSHSHSEKSSVFYQQELPDS
                     DLPRESLKMSAIPGLTDQKTVPTPTVPSGSFSHREKPSIFYQQEWPDSYATEKALKVS
                     TGPGPADQKTEIPAVQSSSYPQREKPSVLYPQVLSDSHLPEESLKVSAFPGPADQMTD
                     TPAVPSTFYSQREKPGIFYQQTLPESHLPKEALKISVAPGLADQKTGTPTVTSTSYSQ
                     HREKPSIFHQQALPGTHIPEEAQKVSAVTGPGNQKTWIPRVLSTFYSQREKPGIFYQQ
                     TLPGSHIPEEAQKVSPVLGPADQKTGTPTPTSASYSHTEKPGIFYQQVLPDNHPTEEA
                     LKISVASEPVDQTTGTPAVTSTSYSQYREKPSIFYQQSLPSSHLTEEAKNVSAVPGPA
                     DQKTVIPILPSTFYSHTEKPGVFYQQVLPHSHPTEEALKISVASEPVDQTTGTPTVTS
                     TSYSQHTEKPSIFYQQSLPGSHLTEEAKNVSAVPGPGDRKTGIPTLPSTFYSHTEKPG
                     SFYQQVLPHSHLPEEALEVSVAPGPVDQTIGTPTVTSPSSSFGEKPIVIYKQAFPEGH
                     LPEESLKVSVAPGPVGQTTGAPTITSPSYSQHRAKSGSFYQLALLGSQIPEEALRVSS
                     APGPADQTTGIPTITSTSYSFGEKPIVNYKQAFPDGHLPEEALKVSIVSGPTEKKTDI
                     PAGPLGSSALGEKPITFYRQALLDSPLNKEVVKVSAAPGPADQKTETLPVHSTSYSNR
                     GKPVIFYQQTLSDSHLPEEALKVPPVPGPDAQKTETPSVSSSLYSYREKPIVFYQQAL
                     PDSELTQEALKVSAVPQPADQKTGLSTVTSSFYSHTEKPNISYQQELPDSHLTEEALK
                     VSNVPGPADQKTGVSTVTSTSYSHREKPIVSYQRELPHFTEAGLKILRVPGPADQKTG
                     INILPSNSYPQREHSVISYEQELPDLTEVTLKAIGVPGPADQKTGIQIASSSSYSNRE
                     KASIFHQQELPDVTEEALNVFVVPGQGDRKTEIPTVPLSYYSRREKPSVISQQELPDS
                     HLTEEALKVSPVSIPAEQKTGIPIGLSSSYSHSHKEKLKISTVHIPDDQKTEFPAATL
                     SSYSQIEKPKISTVIGPNDQKTPSQTAFHSSYSQTVKPNILFQQQLPDRDQSKGILKI
                     SAVPELTDVNTGKPVSLSSSYFHREKSNIFSPQELPGSHVTEDVLKVSTIPGPAGQKT
                     VLPTALPSSFSHREKPDIFYQKDLPDRHLTEDALKISSALGQADQITGLQTVPSGTYS
                     HGENHKLVSEHVQRLIDNLNSSDSSVSSNNVLLNSQADDRVVINKPESAGFRDVGSEE
                     IQDAENSAKTLKEIRTLLMEAENMALKRCNFPAPLARFRDISDISFIQSKKVVCFKEP
                     SSTGVSNGDLLHRQPFTEESPSSRCIQKDIGTQTNLKCRRGIENWEFISSTTVRSPLQ
                     EAESKVSMALEETLRQYQAAKSVMRSEPEGCSGTIGNKIIIPMMTVIKSDSSSDASDG
                     NGSCSWDSNLPESLESVSDVLLNFFPYVSPKTSITDSREEEGVSESEDGGGSSVDSLA
                     AHVKNLLQCESSLNHAKEILRNAEEEESRVRAHAWNMKFNLAHDCGYSISELNEDDRR
                     KVEEIKAELFGHGRTTDLSKGLQSPRGMGCKPEAVCSHIIIESHEKGCFRTLTSEHPQ
                     LDRHPCAFRSAGPSEMTRGRQNPSSCRAKHVNLSASLDQNNSHFKVWNSLQLKSHSPF
                     QNFIPDEFKISKGLRMPFDEKMDPWLSELVEPAFVPPKEVDFHSSSQMPSPEPMKKFT
                     TSITFSSHRHSKCISNSSVVKVGVTEGSQCTGASVGVFNSHFTEEQNPPRDLKQKTSS
                     PSSFKMHSNSQDKEVTILAEGRRQSQKLPVDFERSFQEEKPLERSDFTGSHSEPSTRA
                     NCSNFKEIQISDNHTLISMGRPSSTLGVNRSSSRLGVKEKNVTITPDLPSCIFLEQRE
                     LFEQSKAPRADDHVRKHHSPSPQHQDYVAPDLPSCIFLEQRELFEQCKAPYVDHQMRE
                     NHSPLPQGQDSIASDLPSPISLEQCQSKAPGVDDQMNKHHFPLPQGQDCVVEKNNQHK
                     PKSHISNINVEAKFNTVVSQSAPNHCTLAASASTPPSNRKALSCVHITLCPKTSSKLD
                     SGTLDERFHSLDAASKARMNSEFNFDLHTVSSRSLEPTSKLLTSKPVAQDQESLGFLG
                     PKSSLDFQVVQPSLPDSNTITQDLKTIPSQNSQIVTSRQIQVNISDFEGHSNPEGTPV
                     FADRLPEKMKTPLSAFSEKLSSDAVTQITTESPEKTLFSSEIFINAEDRGHEIIEPGN
                     QKLRKAPVKFASSSSVQQVTFSRGTDGQPLLLPYKPSGSTKMYYVPQLRQIPPSPDSK
                     SDTTVESSHSGSNDAIAPDFPAQVLGTRDDDLSATVNIKHKEGIYSKRVVTKASLPVG
                     EKPLQNENADASVQVLITGDENLSDKKQQEIHSTRAVTEAAQAKEKESLQKDTADSSA
                     AAAAEHSAQVGDPEMKNLPDTKAITQKEEIHRKKTVPEEAWPNNKESLQINIEESECH
                     SEFENTTRSVFRSAKFYIHHPVHLPSDQDICHESLGKSVFMRHSWKDFFQHHPDKHRE
                     HMCLPLPYQNMDKTKTDYTRIKSLSINVNLGNKEVMDTTKSQVRDYPKHNGQISDPQR
                     DQKVTPEQTTQHTVSLNELWNKYRERQRQQRQPELGDRKELSLVDRLDRLAKILQNPI
                     THSLQVSESTHDDSRGERSVKEWSGRQQQRNKLQKKKRFKSLEKSHKNTGELKKSKVL
                     SHHRAGRSNQIKIEQIKFDKYILSKQPGFNYISNTSSDCRPSEESELLTDTTTNILSG
                     TTSTVESDILTQTDREVALHERSSSVSTIDTARLIQAFGHERVCLSPRRIKLYSSITN
                     QQRRYLEKRSKHSKKVLNTGHPLVTSEHTRRRHIQVANHVISSDSISSSASSFLSSNS
                     TFCNKQNVHMLNKGIQAGNLEIVNGAKKHTRDVGITFPTPSSSEAKLEENSDVTSWSE
                     EKREEKMLFTGYPEDRKLKKNKKNSHEGVSWFVPVENVESRSKKENVPNTCGPGISWF
                     EPITKTRPWREPLREQNCQGQHLDGRGYLAGPGREAGRDLLRPFVRATLQESLQFHRP
                     DFISRSGERIKRLKLIVQERKLQSMLQTERDALFNIDRERQGHQNRMCPLPKRVFLAI
                     QKNKPISKKEMIQRSKRIYEQLPEVQKKREEEKRKSEYKSYRLRAQLYKKRVTNQLLG
                     RKVPWD"
     exon            358..483
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     exon            484..679
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     exon            680..797
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     exon            798..1270
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..1371
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     exon            1372..1465
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     exon            1466..7573
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     exon            7574..7707
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     exon            7708..9572
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     exon            9573..9814
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     exon            9815..9940
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     exon            9941..10111
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     exon            10112..10246
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     exon            10247..10417
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     exon            10418..11580
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     exon            11581..11701
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     exon            11702..11905
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     exon            11906..12147
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     exon            12148..12331
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     exon            12332..12395
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     exon            12396..12495
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     exon            12496..12844
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      12826..12831
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /note="hexamer: AATAAA"
     polyA_site      12844
                     /gene="ALMS1"
                     /gene_synonym="ALSS"
                     /note="major polyA site"
ORIGIN      
        1 ctcctcctct gccgcccaga gcgagacacc aacatggagc ccgaggatct gccatggccg
       61 ggcgagctgg aggaggagga ggaggaggag gaggaggagg aggaggaaga ggaggaggct
      121 gcagcggcgg cggcggcgaa cgtggacgac gtagtggtcg tggaggaggt ggaggaagag
      181 gcggggcggg agttggactc cgactctcac tacgggcccc agcatctgga aagtatagac
      241 gacgaggagg acgaggaggc caaggcctgg ctgcaggcgc accccggcag gattttgcct
      301 ccgctgtcgc ccccgcagca ccgctactcg gagggcgagc ggacctccct ggagaagatt
      361 gttccattga cctgtcatgt atggcaacag atagtatatc aaggcaatag tagaacacaa
      421 atttctgata ctaatgtggt ctgtttggaa acaacagctc agcggggttc tggggatgat
      481 cagaaaacag aatcttggca ttgtcttcct caagaaatgg actcttccca aaccttggat
      541 acatcccaga ctaggtttaa tgtgagaacg gaagatactg aagtgacaga cttcccctct
      601 ctggaggagg gcatattgac gcaatcagaa aatcaagtaa aggaacccaa cagagatctc
      661 ttctgttctc cactgctagt catacaagat agctttgctt ctcctgattt gcctttgctg
      721 acctgtttga cacaagacca agaatttgcg cctgattctt tatttcatca aagtgaacta
      781 agttttgcac ctctgagggg aattcctgat aagtctgaag atactgaatg gtcttctcga
      841 ccatcggaag ttagtgaagc tttattccag gctactgcag aagtagcttc agacttagca
      901 agcagtcgct ttagtgtatc tcagcacccg cttataggca gcacagctgt tgggtctcag
      961 tgcccttttt taccttctga acaagggaat aatgaagaga ctatttcgtc tgttgatgaa
     1021 ctgaaaattc ccaaagactg tgatcgttat gatgatcttt gttcatatat gtcatggaag
     1081 acacgaaaag atacacagtg gcctgaaaac aatttagctg ataaagatca agtttcagtt
     1141 gcaacttcat ttgacataac tgatgaaaac atagctacta aaagaagtga ccattttgat
     1201 gctgctcgtt catatgggca gtattggaca caggaagatt catctaagca ggcagaaaca
     1261 tatttaacca agggcctgca ggggaaggtt gagtctgacg tcattactct ggatggccta
     1321 aatgaaaatg ctgttgtatg cagtgaaaga gttgctgaac tacaaagaaa gccaacaaga
     1381 gagtcggaat atcactcttc agatctcaga atgttgagga tgtctcctga cactgtgcca
     1441 aaggctccta aacatttaaa agcaggagac acttctaaag gaggcatagc taaagttact
     1501 caatccaact tgaagtcagg catcactacc actcctgttg attcagacat tggatctcat
     1561 ttatccttgt cccttgagga cctgtctcag ttggctgtaa gttctcctct agaaactact
     1621 actggtcaac acactgatac tctcaaccaa aagacattag cagatactca tctaactgaa
     1681 gagactctga aagtcacagc tattcctgaa ccagctgacc agaagactgc aacaccaaca
     1741 gtactctcta gttcccactc acataggggg aagcccagca ttttctacca gcagggcttg
     1801 ccagacagtc atctaactga agaggctttg aaagtttcag ctgctcctgg actagctgac
     1861 cagacaactg gcatgtcaac tctaacctct acttcctact cacatagaga gaagcctggt
     1921 actttttacc aacaagagtt accagagagt aacttaaccg aagagccttt ggaagtttca
     1981 gctgctcctg gcccagtgga gcagaagacg ggaataccta cagtatcctc tacatcccac
     2041 tcacatgtag aggacctcct ctttttctat cgacagacct tgccagatgg tcatctaact
     2101 gatcaggctc tgaaagtctc agctgtgtct ggaccagctg accagaagac tgggacagca
     2161 acagtactct ctactcccca ctcacataga gagaagcctg gtatttttta ccaacaagag
     2221 ttcgcagaca gtcatcaaac tgaagagact cttactaaag tttcagccac tcctggacca
     2281 gctgaccaga agactgagat accagcagta cagtctagtt cttactcaca aagagaaaag
     2341 cctagtattt tgtacccaca ggacttagca gacagtcatc tacctgaaga gggtctgaaa
     2401 gtttcagctg ttgctggacc agctgaccag aagactggcc taccaacagt accctctagt
     2461 gcatactcac acagagagaa gctccttgtt ttctaccaac aggccttgct ggacagccat
     2521 ctacccgaag aggctctgaa agtttcagct gtttctggac cagctgacgg aaagactggg
     2581 acaccagctg taacctctac ttcctctgcg tcctcttcac ttggagaaaa gcccagtgct
     2641 ttctatcagc agaccttacc caatagtcat ctaactgaag aggctctgaa agtatcaatt
     2701 gttcctggac caggtgatca gaagactggg ataccctcag caccatctag tttctactca
     2761 cacagagaga agcccattat tttttcccag cagaccctgc cagactttct tttccctgaa
     2821 gaagctctga aggtttcagc tgtttctgta ttggctgccc agaagactgg gacaccaaca
     2881 gtgtcctcta attctcactc acatagcgag aaatctagtg ttttctacca gcaagagttg
     2941 ccagacagtg atctacctag agaatctctg aaaatgtctg ctattcctgg actgactgac
     3001 cagaagactg tcccaacacc aacagtacct tcaggttcct tctcacatag agagaagccc
     3061 agtattttct atcaacagga gtggccagat agttatgcaa ctgaaaaggc tctgaaagtt
     3121 tcaactggcc ctggaccagc tgaccagaag actgagatac cagcagtaca gtctagttct
     3181 tacccacaga gggagaagcc tagtgttttg tacccacagg tgttatcaga cagtcatcta
     3241 cctgaagaga gtctgaaagt ttcagccttc cctggaccag ctgaccagat gactgacaca
     3301 ccagcagtac cgtctacttt ctactcacaa agagagaagc ctggtatttt ctaccaacag
     3361 accttgccag agagtcatct gcctaaagag gctctgaaaa tttcagtagc tcctggacta
     3421 gcagaccaga agactggcac accaactgta acctcaactt cctactcaca acatagagaa
     3481 aagcccagca ttttccacca gcaggccttg ccaggtactc atatacctga agaggctcag
     3541 aaagtttcag ctgttactgg accaggtaac cagaagactt ggataccaag agtactttct
     3601 accttctact cacaaagaga gaaacctggt attttctatc aacagacctt gccaggtagt
     3661 cacatacctg aagaggcaca gaaagtttca cctgttcttg gaccagctga ccagaagact
     3721 gggacaccaa ctccaacctc tgcttcttac tcacacacag agaagcctgg tattttctac
     3781 caacaggtct tgccagataa tcatccaact gaagaggctc tgaaaatttc agttgcctct
     3841 gaaccagttg accagacaac tggcacacca gctgtaacct ctacttccta ctcacaatat
     3901 agagagaagc ccagcatttt ctaccaacag tcgttgccaa gtagtcatct aactgaagag
     3961 gctaagaatg tttcagcggt tcctggacca gctgaccaga agactgtgat accaatttta
     4021 ccctctactt tctactcaca cacagagaag cctggtgttt tctaccaaca ggtcttgcca
     4081 catagtcatc caactgaaga ggctctgaaa atttcagttg cctctgaacc agttgaccag
     4141 acaactggca caccaactgt aacctctact tcttactcac aacatacaga gaagccgagt
     4201 attttctacc aacagtcgtt gccaggtagt catctaactg aagaggctaa gaacgtttca
     4261 gcggttcctg gaccaggtga ccggaagact gggataccaa ctttaccctc tactttctac
     4321 tcacacacag agaagcctgg tagtttctac caacaggtct tgccacatag tcatctacct
     4381 gaagaggctt tggaagtttc agttgctcct ggaccagttg accagacgat tggcacacca
     4441 actgtaacct ccccttccag ctcatttgga gagaagccca ttgttatcta caaacaggcc
     4501 tttccagagg gtcatctacc tgaagagtct ctgaaagttt cagttgctcc tggaccagtt
     4561 ggccagacaa ctggcgcacc aactataacc tctccttcct actcacaaca tagagcaaag
     4621 tctggcagtt tctaccaact ggcattgcta ggtagtcaaa tacctgaaga ggctctcaga
     4681 gtttcttctg ctcctggacc agctgaccag acaactggca taccaaccat aacctctact
     4741 tcctactcat ttggagagaa gccgattgtt aactacaaac aggcctttcc agatggtcat
     4801 ctacctgaag aggctctgaa agtttccatt gtttctggac ctactgaaaa aaagactgac
     4861 ataccagcag gacctttagg ttccagtgca cttggagaga agcccattac tttctaccgg
     4921 caggctctgc tagacagtcc tctaaataaa gaggttgtga aagtttcagc tgctcctgga
     4981 ccagctgacc agaagactga gacattacca gtacattcta ctagctactc aaataggggg
     5041 aagcctgtca ttttctacca gcagacccta tcagacagtc atttacctga agaagctctg
     5101 aaagttccac ctgttcctgg accagatgcc cagaagactg agacaccatc agtatcctct
     5161 agtttatact catatagaga gaagcccatt gtcttctacc aacaggccct gccagacagt
     5221 gagctaactc aagaagctct gaaagtttca gctgttcctc aaccagctga ccagaagact
     5281 gggttatcta ctgtaacttc ctctttctat tcacatacag agaagcctaa tatttcttac
     5341 cagcaagagt tgccagatag tcatctaact gaagaggctc tgaaagtttc aaatgttcct
     5401 ggaccagctg accagaagac tggggtatca acagtaacct ctacttccta ctcacacaga
     5461 gagaagccca ttgtttccta ccagcgagag ttgccgcatt ttactgaagc aggtttgaaa
     5521 attttaagag ttcctggacc agctgaccag aagactggaa taaacatcct gccctctaat
     5581 tcctacccac agagagagca ctctgtcatt tcttatgagc aggagttgcc agatcttact
     5641 gaagtaactt tgaaagcaat aggggttcct gggcctgctg accagaagac tgggatacaa
     5701 atagcatcct ctagttccta ctcaaataga gagaaggcca gtatttttca tcagcaggag
     5761 ttgccagatg ttactgaaga agctttaaat gtttttgttg ttcctggaca aggtgaccgg
     5821 aagactgaga taccaacagt acctttaagt tactactcac gtagagagaa gcccagtgtt
     5881 atctctcaac aggagttgcc agacagtcat ctcacagaag aggctctgaa agtttcacct
     5941 gtttctatac cagcagagca gaagactggg ataccaatag gactgtctag ttcctactca
     6001 cattcacata aagagaaact caagatttca actgtgcata taccagatga ccagaaaact
     6061 gagtttccag cagctaccct tagttcctac tcacaaatag agaagcccaa gatttcaact
     6121 gtgattggac caaatgacca gaagactcca tcccagacag cttttcatag ttcctattct
     6181 caaacagtaa agcccaatat tttatttcaa cagcagttgc cagatagaga tcaaagtaaa
     6241 ggtattctaa agatttcagc tgtccctgaa ctaactgatg tgaatactgg aaaaccagta
     6301 tctctctcta gttcttattt tcacagagag aaatcgaata ttttcagtcc acaggaattg
     6361 ccaggtagtc atgtaactga agatgtgctg aaggtttcaa caattcctgg accagctggc
     6421 cagaaaacag tattaccaac agctcttcct agttcctttt cacatcgaga gaaaccagat
     6481 attttctatc aaaaggattt gccagataga catctaactg aagatgctct aaagatctca
     6541 agtgctcttg ggcaagctga tcaaattacc ggattacaaa cagttccctc tggtacttac
     6601 tcacatggtg agaatcacaa gcttgtttca gaacatgtcc aaaggctaat agataatttg
     6661 aattcttctg actccagtgt tagctcaaat aatgtgcttt taaattctca ggctgatgac
     6721 agagttgtaa taaataaacc agaatctgca ggttttagag atgttggctc tgaagaaatc
     6781 caggatgcag aaaatagtgc taaaactctt aaggaaattc ggacactttt gatggaggca
     6841 gaaaatatgg cactgaaacg atgcaatttt cctgctcccc ttgcccgttt cagagatatt
     6901 agtgatattt catttataca atctaagaag gtggtttgct tcaaagaacc ctcttccacg
     6961 ggtgtatcta atggtgattt gcttcacaga cagccattca cagaggaaag cccaagcagc
     7021 aggtgcatac agaaggatat tggcacacag acgaatttga aatgccggag aggcattgaa
     7081 aattgggagt ttattagttc aactacagtt agaagtcctc tacaggaagc agagagcaaa
     7141 gtcagtatgg cattagaaga aactcttagg caatatcaag cagccaaatc tgtaatgagg
     7201 tctgaacctg aagggtgtag tggaaccatt gggaataaaa ttattatccc tatgatgact
     7261 gtcataaaaa gtgattcaag tagtgatgcc agtgatggaa atggttcctg ctcgtgggac
     7321 agtaatttac cagagtcttt ggaatcagtt tctgatgttc ttctaaactt ctttccatat
     7381 gtttcaccca agacaagtat aacagatagc agggaggaag agggtgtgtc agagagtgag
     7441 gatggtggtg gtagcagtgt agattcactg gctgcacatg tgaaaaacct tctgcaatgt
     7501 gaatcctcac tgaatcatgc taaagaaata ctcagaaatg cagaggaaga ggaaagccgg
     7561 gtacgagcac atgcctggaa tatgaagttc aatttagcac atgattgtgg atactccatt
     7621 tcagaattaa atgaagatga caggaggaaa gtagaagaga tcaaggcaga gttatttggt
     7681 catggaagaa caactgactt gtccaagggt ttacagagtc cacggggaat gggatgcaag
     7741 ccagaagctg tatgtagtca cattattatt gagagccatg aaaagggatg tttccggact
     7801 ctaacttctg aacatccaca actagataga cacccttgtg ctttcagatc tgctggaccc
     7861 tcagaaatga ccagaggacg gcagaaccca tcatcatgca gagccaagca tgtcaacctt
     7921 tctgcatcct tagaccagaa caactcccat ttcaaagttt ggaattcctt gcagttaaaa
     7981 agtcattccc catttcagaa ctttatacct gatgaattca aaatcagcaa aggtcttcga
     8041 atgccattcg atgaaaagat ggacccttgg ctgtcagaat tagtagaacc tgcttttgtg
     8101 ccacctaaag aagtggattt tcattcttca tcacaaatgc cgtccccaga acccatgaaa
     8161 aagtttacta cctccatcac tttttcatct caccgacatt ctaaatgcat ttccaattcc
     8221 tctgttgtta aggttggtgt tactgaaggt agccagtgta ctggagcatc tgtgggggta
     8281 tttaattctc atttcactga agaacaaaat cctcccagag atcttaaaca gaaaacctct
     8341 tccccttcat catttaaaat gcatagtaat tcacaagata aagaagtgac tattttagca
     8401 gaaggtagaa ggcaaagcca aaaattacct gttgattttg agcgttcttt tcaagaagaa
     8461 aaacccttag aaagatcaga ttttacaggc agtcattctg agcccagtac cagggcaaat
     8521 tgtagcaatt tcaaggaaat tcagatttct gataaccata cccttattag catgggcaga
     8581 ccaagttcca ccctaggagt aaacagatcg agttccagac taggagtaaa agagaagaat
     8641 gtaactataa ctccagatct tccttcttgc atttttcttg aacaacgaga gctctttgaa
     8701 caaagcaaag ccccacgtgc agatgaccat gtgaggaaac accattctcc ctctcctcaa
     8761 catcaggatt atgtagctcc agaccttcct tcttgcattt ttcttgaaca acgagaactc
     8821 tttgaacagt gcaaagcccc atatgtagat catcaaatga gagaaaacca ttctcccctt
     8881 cctcaaggtc aggattctat agcttcagac cttccgtctc ccatttctct tgaacaatgc
     8941 caaagcaaag cgccaggtgt agatgaccaa atgaataaac accattttcc ccttcctcaa
     9001 ggtcaggatt gtgtagtgga aaagaataat caacataagc ctaaatcaca catttctaat
     9061 ataaatgttg aagccaagtt caatactgtg gtctcccagt cagccccaaa tcactgtaca
     9121 ttagcagcat ctgcatctac tcctccttca aatagaaaag cactttcttg tgttcatata
     9181 actctttgtc ccaagacttc ttccaagttg gatagtggaa ctttagatga aagattccat
     9241 tcattggatg ctgcttctaa agcgaggatg aatagtgagt ttaactttga cttacatact
     9301 gtatcttcga gatcactgga accaacctcc aaattattga ccagtaaacc tgtagcacag
     9361 gatcaagaat ctttaggttt tctaggacct aaatcttcac tggatttcca agtcgtacag
     9421 ccttctcttc cagacagtaa cactattact caggacttga aaaccatacc ttctcagaat
     9481 agccagatag taacctccag gcaaatacaa gtgaacattt cagatttcga aggacattcc
     9541 aatccagagg ggaccccagt atttgcagat cgattaccag agaagatgaa gaccccactt
     9601 tctgctttct ctgaaaaatt gtcatctgat gcagtcactc agataacaac agaaagtcca
     9661 gaaaagaccc tattttcatc tgagattttt attaatgctg aagatcgtgg acatgaaatt
     9721 atagagcctg gtaaccagaa gctacgcaaa gctcctgtca agtttgcctc atcatcttca
     9781 gtccaacagg ttactttttc tcgcggcaca gatggccagc ctttattatt gccatataag
     9841 ccttctggta gtaccaagat gtattatgtt ccacaattaa gacaaattcc tccatctccg
     9901 gattccaaat cagataccac cgttgaaagc tcccattcag gatccaatga tgccattgct
     9961 ccagacttcc cagctcaggt gctaggcaca agagatgatg acctctcagc cactgttaac
    10021 attaaacata aagaaggaat ctacagtaag agggtagtga ctaaggcatc cttgccagtg
    10081 ggagaaaaac ccttgcagaa tgaaaatgca gatgcctcag ttcaagtgct aatcactggg
    10141 gatgagaacc tctcagacaa aaaacagcaa gagattcaca gtacaagggc agtgactgag
    10201 gctgcccagg ctaaagaaaa agaatctttg cagaaagata ctgcagattc cagtgctgct
    10261 gctgctgcag agcactcagc tcaagtagga gacccagaaa tgaagaactt gccagacact
    10321 aaagccatta cacagaaaga ggagatccat aggaagaaga cagttcccga ggaagcctgg
    10381 ccaaacaata aagaatccct acagatcaat attgaagagt ccgaatgtca ttcagaattt
    10441 gaaaatacta cccgttctgt cttcaggtca gcaaagtttt acattcatca tcccgtacac
    10501 ctaccaagtg atcaagatat ttgccatgaa tctttgggaa agagtgtttt catgagacat
    10561 tcttggaaag atttctttca gcatcatcca gacaaacata gagaacacat gtgtcttcct
    10621 cttccttatc aaaacatgga caagactaag acagattata ccagaataaa gagcctcagc
    10681 atcaatgtga atttgggaaa caaagaagtg atggatacta ctaaaagtca agttagagat
    10741 tatccaaaac ataatggaca aattagtgat ccacaaaggg atcagaaggt caccccagag
    10801 caaacaactc agcacactgt gagtttgaat gaactgtgga acaagtatcg ggagcgacag
    10861 aggcaacaga gacagcctga gttgggtgac aggaaagaac tgtccttggt ggaccgactt
    10921 gatcgtttgg ctaaaattct tcagaatcca atcacacatt ctctccaggt ctcagaaagt
    10981 acacatgatg atagcagagg ggaacgaagt gtgaaggaat ggagtggtag acaacagcag
    11041 agaaataagc ttcagaaaaa gaagcggttt aaaagcctag agaaaagcca taaaaataca
    11101 ggcgagctta aaaaaagcaa ggtgctttct catcatcgag ctgggaggtc taatcaaatt
    11161 aaaattgaac agattaaatt tgataaatat attctgagta aacagccagg ttttaattat
    11221 ataagcaaca cttcttcgga ttgtcggccc tcagaggaga gtgagctgct cacagatact
    11281 accaccaaca tcctttccgg caccacttct actgtcgaat cagatatatt gacccaaaca
    11341 gatagagagg tggctctgca cgaaaggagt agctctgttt ccactattga cactgcccgg
    11401 ctgattcaag cttttggcca tgaaagagta tgcttgtcac ccagacgaat taaattatat
    11461 agcagcatca ccaaccaaca gaggagatac cttgagaagc ggagcaaaca cagcaagaaa
    11521 gtgctgaata caggtcatcc cctagtgact tctgagcaca ccagaaggag acacatccag
    11581 gtagcaaacc atgtgatttc ttctgactct atttcctctt ctgccagtag tttcctgagc
    11641 tcaaactcta ctttttgcaa caagcagaat gtacacatgt taaacaaggg catacaagca
    11701 ggtaacttgg agattgtgaa cggtgccaaa aaacacactc gagatgttgg gataactttc
    11761 ccaactccaa gttccagcga ggctaaattg gaagagaaca gtgatgtgac ttcttggtca
    11821 gaagaaaaac gtgaagagaa aatgctcttt accggttatc ctgaggacag aaagttaaaa
    11881 aagaacaaga agaattccca tgaaggagtt tcctggtttg ttcctgtgga aaatgtggag
    11941 tctagatcaa agaaggaaaa cgtgcctaac acttgtggcc ctggcatctc ctggtttgaa
    12001 ccaataacca agaccagacc ctggagggag ccactgcggg agcagaactg tcaggggcag
    12061 cacctggacg gtcggggcta cctggcaggc ccaggcagag aggctggcag agacctactg
    12121 aggccatttg tgagagcaac ccttcaggaa tcgcttcagt ttcacagacc tgacttcatc
    12181 tcccgctctg gggagcggat aaagcgcctg aagttaatag tccaggagag gaagctgcag
    12241 agcatgttac agaccgagcg ggatgcacta ttcaacattg acagggaacg gcagggccac
    12301 cagaatcgca tgtgcccgct gcccaagaga gtcttcctgg ctatccagaa gaacaagcct
    12361 atcagcaaga aggaaatgat tcagaggtcc aaacggattt atgagcagct tccagaagta
    12421 cagaaaaaga gagaagaaga gaagagaaaa tcagaatata agtcataccg gctgcgagcc
    12481 cagctatata aaaagagagt gaccaatcaa cttctgggga gaaaagttcc ctgggactga
    12541 cacaagttta ttttcctcag agccttggaa ttctatttta tgaacctaga gaagcagaat
    12601 ccttactttt gtgagtctgg ttgaataaag cttattcttt gtccatgtgt attttagaaa
    12661 tagtaacttc taaagagtct ggaacaaagt ggtgattaaa attcctaatg gtttgggagc
    12721 aatactttct gcatagtggc cttgtccaat ggcctgtgtg ttacaatgat atgatcattt
    12781 ctcaagaata agtccctttt tgtatgtgtt tttatacttt tagaaaataa aaactttaga
    12841 ttaa
//
