LOCUS       NM_017621               2092 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens alkB homolog 4, lysine demethylase (ALKBH4), mRNA.
ACCESSION   NM_017621
VERSION     NM_017621.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Kogaki,T., Hase,H., Tanimoto,M., Tashiro,A., Kitae,K., Ueda,Y.,
            Jingushi,K. and Tsujikawa,K.
  TITLE     ALKBH4 is a novel enzyme that promotes translation through modified
            uridine regulation
  JOURNAL   J Biol Chem 299 (9), 105093 (2023)
   PUBMED   37507018
  REMARK    GeneRIF: ALKBH4 is a novel enzyme that promotes translation through
            modified uridine regulation.
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Yu,K., Qi,T.F., Miao,W., Liu,X. and Wang,Y.
  TITLE     Quantitative proteomics revealed new functions of ALKBH4
  JOURNAL   Proteomics 22 (7), e2100231 (2022)
   PUBMED   34951099
  REMARK    GeneRIF: Quantitative proteomics revealed new functions of ALKBH4.
REFERENCE   3  (bases 1 to 2092)
  AUTHORS   Jingushi,K., Aoki,M., Ueda,K., Kogaki,T., Tanimoto,M., Monoe,Y.,
            Ando,M., Matsumoto,T., Minami,K., Ueda,Y., Kitae,K., Hase,H.,
            Nagata,T., Harada-Takeda,A., Yamamoto,M., Kawahara,K., Tabata,K.,
            Furukawa,T., Sato,M. and Tsujikawa,K.
  TITLE     ALKBH4 promotes tumourigenesis with a poor prognosis in
            non-small-cell lung cancer
  JOURNAL   Sci Rep 11 (1), 8677 (2021)
   PUBMED   33883577
  REMARK    GeneRIF: ALKBH4 promotes tumourigenesis with a poor prognosis in
            non-small-cell lung cancer.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2092)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 2092)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2092)
  AUTHORS   Li,M.M., Nilsen,A., Shi,Y., Fusser,M., Ding,Y.H., Fu,Y., Liu,B.,
            Niu,Y., Wu,Y.S., Huang,C.M., Olofsson,M., Jin,K.X., Lv,Y., Xu,X.Z.,
            He,C., Dong,M.Q., Rendtlew Danielsen,J.M., Klungland,A. and
            Yang,Y.G.
  TITLE     ALKBH4-dependent demethylation of actin regulates actomyosin
            dynamics
  JOURNAL   Nat Commun 4, 1832 (2013)
   PUBMED   23673617
  REMARK    GeneRIF: ALKBH4-dependent actin demethylation regulates actomyosin
            function by promoting actin-non-muscle myosin II interaction.
REFERENCE   7  (bases 1 to 2092)
  AUTHORS   Bjornstad,L.G., Meza,T.J., Otterlei,M., Olafsrud,S.M.,
            Meza-Zepeda,L.A. and Falnes,P.O.
  TITLE     Human ALKBH4 interacts with proteins associated with transcription
  JOURNAL   PLoS One 7 (11), e49045 (2012)
   PUBMED   23145062
  REMARK    GeneRIF: The regions mediating binding to ALKBH4 comprised domains
            previously reported to be involved in interaction with DNA or
            chromatin.
REFERENCE   8  (bases 1 to 2092)
  AUTHORS   Yu,H., Tardivo,L., Tam,S., Weiner,E., Gebreab,F., Fan,C.,
            Svrzikapa,N., Hirozane-Kishikawa,T., Rietman,E., Yang,X.,
            Sahalie,J., Salehi-Ashtiani,K., Hao,T., Cusick,M.E., Hill,D.E.,
            Roth,F.P., Braun,P. and Vidal,M.
  TITLE     Next-generation sequencing to generate interactome datasets
  JOURNAL   Nat Methods 8 (6), 478-480 (2011)
   PUBMED   21516116
REFERENCE   9  (bases 1 to 2092)
  AUTHORS   Bjornstad,L.G., Zoppellaro,G., Tomter,A.B., Falnes,P.O. and
            Andersson,K.K.
  TITLE     Spectroscopic and magnetic studies of wild-type and mutant forms of
            the Fe(II)- and 2-oxoglutarate-dependent decarboxylase ALKBH4
  JOURNAL   Biochem J 434 (3), 391-398 (2011)
   PUBMED   21166655
  REMARK    GeneRIF: ALKBH4 represents an active Fe(II)/2OG-dependent
            decarboxylase and suggest that the cysteine cluster is involved in
            processes other than Fe co-ordination.
REFERENCE   10 (bases 1 to 2092)
  AUTHORS   Tsujikawa,K., Koike,K., Kitae,K., Shinkawa,A., Arima,H., Suzuki,T.,
            Tsuchiya,M., Makino,Y., Furukawa,T., Konishi,N. and Yamamoto,H.
  TITLE     Expression and sub-cellular localization of human ABH family
            molecules
  JOURNAL   J Cell Mol Med 11 (5), 1105-1116 (2007)
   PUBMED   17979886
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX353099.2 and BC017096.1.
            
            On Nov 23, 2018 this sequence version replaced NM_017621.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.1003033.1,
                                           SRR3476690.179501.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2151358, SAMN01820687
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000292566.4/ ENSP00000292566.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                BX353099.2         12-33
            23-2092             BC017096.1         1-2070
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q22.1"
     gene            1..2092
                     /gene="ALKBH4"
                     /gene_synonym="ABH4"
                     /note="alkB homolog 4, lysine demethylase"
                     /db_xref="GeneID:54784"
                     /db_xref="HGNC:HGNC:21900"
                     /db_xref="MIM:613302"
     exon            1..150
                     /gene="ALKBH4"
                     /gene_synonym="ABH4"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..936
                     /gene="ALKBH4"
                     /gene_synonym="ABH4"
                     /EC_number="1.14.11.51"
                     /note="alkylated DNA repair protein alkB homolog 4;
                     probable alpha-ketoglutarate-dependent dioxygenase ABH4;
                     alkB, alkylation repair homolog 4; alkB homolog 4, lysine
                     demthylase; lysine-specific demethylase ALKBH4; DNA
                     N6-methyl adenine demethylase ALKBH4"
                     /codon_start=1
                     /product="alpha-ketoglutarate-dependent dioxygenase alkB
                     homolog 4"
                     /protein_id="NP_060091.1"
                     /db_xref="CCDS:CCDS5723.1"
                     /db_xref="GeneID:54784"
                     /db_xref="HGNC:HGNC:21900"
                     /db_xref="MIM:613302"
                     /translation="MAAAAAETPEVLRECGCKGIRTCLICERQRGSDPPWELPPAKTY
                     RFIYCSDTGWAVGTEESDFEGWAFPFPGVMLIEDFVTREEEAELVRLMDRDPWKLSQS
                     GRRKQDYGPKVNFRKQKLKTEGFCGLPSFSREVVRRMGLYPGLEGFRPVEQCNLDYCP
                     ERGSAIDPHLDDAWLWGERLVSLNLLSPTVLSMCREAPGSLLLCSAPSAAPEALVDSV
                     IAPSRSVLCQEVEVAIPLPARSLLVLTGAARHQWKHAIHRRHIEARRVCVTFRELSAE
                     FGPGGRQQELGQELLRIALSFQGRPV"
     exon            151..348
                     /gene="ALKBH4"
                     /gene_synonym="ABH4"
                     /inference="alignment:Splign:2.1.0"
     exon            349..2092
                     /gene="ALKBH4"
                     /gene_synonym="ABH4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1779..1784
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ALKBH4"
                     /gene_synonym="ABH4"
                     /note="hexamer: ATTAAA"
     polyA_site      1811
                     /gene="ALKBH4"
                     /gene_synonym="ABH4"
                     /note="major polyA site"
     regulatory      2072..2077
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ALKBH4"
                     /gene_synonym="ABH4"
                     /note="hexamer: AATAAA"
     polyA_site      2092
                     /gene="ALKBH4"
                     /gene_synonym="ABH4"
                     /note="major polyA site"
ORIGIN      
        1 ctgcgcactg gccacgcgga cggcgcgatg gcggcggctg ccgccgagac ccccgaagtc
       61 cttcgggaat gcggttgcaa gggcatccgg acctgtctga tctgcgagcg gcagcgcggc
      121 agtgacccgc cctgggagct gcccccagcg aaaacatacc gtttcattta ctgctccgac
      181 accggctggg ccgtgggcac agaggagtct gactttgagg gctgggcctt ccccttccca
      241 ggagtgatgc tgatcgagga ctttgtgacc cgggaggaag aagccgagtt ggtgcggctc
      301 atggaccgtg acccctggaa gctctcccag tctggacgga ggaagcagga ctatggcccc
      361 aaagtcaact ttcggaaaca gaagctaaag accgagggct tctgcggcct ccccagcttc
      421 agccgggagg tggtgcggag gatgggcctc tacccggggc tggagggctt ccggcccgtc
      481 gagcagtgca acctggacta ctgccccgag cggggctctg ccattgaccc ccacctggac
      541 gacgcctggc tgtgggggga gcggctggtc agcctcaacc tcctgtcccc caccgtgctg
      601 tccatgtgtc gggaggcgcc cgggagcctg ctcctctgct cggccccgtc ggctgccccg
      661 gaggccttgg tggacagcgt gatagcaccc agccggtcgg tgctatgcca ggaggtggag
      721 gtggccatcc ccttacccgc ccgctccctg ctggtcctca ccggggcggc acggcaccag
      781 tggaagcatg ccatccaccg cagacacatc gaggcccgcc gcgtctgcgt cactttccgg
      841 gagctgtcgg ctgagtttgg ccctggaggg aggcagcaag agctgggcca ggaactgctg
      901 cggatcgccc tctccttcca gggaagaccc gtgtgaaccg cctccttggc tccagacttg
      961 actgatcccg ggattgaaat gaggagcaca gaacagggcc tcctgcaact cacggggttt
     1021 caagagaaga tggctgaccc ctgatgctgt gagcagtgtg agccctgccc aggagcaggt
     1081 tttgatggga acgtacctcc aggcagcccc cttccacctg gaccgtggcc acactttttt
     1141 ggttatttag tttgtcacag tcttggggac atgggatcat ttgagcttaa aaaatactgg
     1201 gggccgggca cagtggctca cacctgtaat cctaacactt tgggaggctg aggtgggcgg
     1261 atcacttgat gccaggagtt cgagaccagc ctggccaaca cggtgaaaac ccgtctctac
     1321 aaaaactaca aaaattagcc gggtgtggtg actcacagcc gtaatcccag ctactcggga
     1381 ggctaaggtg ggagaattgc ttgaacctgg gaggcggagg ttgcagtgag ccaagatcac
     1441 gccactgcac tccagcctcg gtgacagagc aagactgttt tgaaaaaaaa aaaaaatggg
     1501 aacattttaa atgattttca cctttattat gcatctattt tcatggggtt tcccgatatc
     1561 tcactgtcca gtcccttcat ttggggaatg tgttggatta gggaacaggg ttgaagattt
     1621 gaagtttaga ctaaagagct gggaacagct tcagagtcag gctcagcctg actcatgctt
     1681 gacaccccca cgcccaggga gggttggggg atgtgaggag ggcagggaaa tctgagagcc
     1741 tccttccagc cccataacgc tgttaacaag taggaaaaat taaagctccc ggccaggcgc
     1801 ggtgactcac acctgtaatc cgagtacttt gcggggctca ggtgggagga ttgcttgagg
     1861 ccagcctggg caacatagtg agacccccat ctctacaaaa aatacaaaca ttagctgggc
     1921 gtctgggcat ggtggcacac acctgtagtc ccagctactc gaaaggctga ggcgggagga
     1981 tggctttacc accatgtcaa ggctgcagtg agctcatgat cataccactg cacttaactt
     2041 ggcaacagag caagaccctg tccctaaaat aaataaaagg aaaacaaaaa aa
//
