LOCUS       NM_001002919            1184 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens ALK and LTK ligand 2 (ALKAL2), mRNA.
ACCESSION   NM_001002919 XM_211736
VERSION     NM_001002919.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Mazzeschi,M., Sgarzi,M., Romaniello,D., Gelfo,V., Cavallo,C.,
            Ambrosi,F., Morselli,A., Miano,C., Laprovitera,N., Girone,C.,
            Ferracin,M., Santi,S., Rihawi,K., Ardizzoni,A., Fiorentino,M.,
            D'Uva,G., Gyorffy,B., Palmer,R. and Lauriola,M.
  TITLE     The autocrine loop of ALK receptor and ALKAL2 ligand is an
            actionable target in consensus molecular subtype 1 colon cancer
  JOURNAL   J Exp Clin Cancer Res 41 (1), 113 (2022)
   PUBMED   35351152
  REMARK    GeneRIF: The autocrine loop of ALK receptor and ALKAL2 ligand is an
            actionable target in consensus molecular subtype 1 colon cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Reshetnyak,A.V., Rossi,P., Myasnikov,A.G., Sowaileh,M., Mohanty,J.,
            Nourse,A., Miller,D.J., Lax,I., Schlessinger,J. and Kalodimos,C.G.
  TITLE     Mechanism for the activation of the anaplastic lymphoma kinase
            receptor
  JOURNAL   Nature 600 (7887), 153-157 (2021)
   PUBMED   34819673
REFERENCE   3  (bases 1 to 1184)
  AUTHORS   De Munck,S., Provost,M., Kurikawa,M., Omori,I., Mukohyama,J.,
            Felix,J., Bloch,Y., Abdel-Wahab,O., Bazan,J.F., Yoshimi,A. and
            Savvides,S.N.
  TITLE     Structural basis of cytokine-mediated activation of ALK family
            receptors
  JOURNAL   Nature 600 (7887), 143-147 (2021)
   PUBMED   34646012
REFERENCE   4  (bases 1 to 1184)
  AUTHORS   Borenas,M., Umapathy,G., Lai,W.Y., Lind,D.E., Witek,B., Guan,J.,
            Mendoza-Garcia,P., Masudi,T., Claeys,A., Chuang,T.P., El Wakil,A.,
            Arefin,B., Fransson,S., Koster,J., Johansson,M., Gaarder,J., Van
            den Eynden,J., Hallberg,B. and Palmer,R.H.
  TITLE     ALK ligand ALKAL2 potentiates MYCN-driven neuroblastoma in the
            absence of ALK mutation
  JOURNAL   EMBO J 40 (3), e105784 (2021)
   PUBMED   33411331
  REMARK    GeneRIF: ALK ligand ALKAL2 potentiates MYCN-driven neuroblastoma in
            the absence of ALK mutation.
REFERENCE   5  (bases 1 to 1184)
  AUTHORS   Reshetnyak,A.V., Mohanty,J., Tome,F., Puleo,D.E., Plotnikov,A.N.,
            Ahmed,M., Kaur,N., Poliakov,A., Cinnaiyan,A.M., Lax,I. and
            Schlessinger,J.
  TITLE     Identification of a biologically active fragment of ALK and
            LTK-Ligand 2 (augmentor-alpha)
  JOURNAL   Proc Natl Acad Sci U S A 115 (33), 8340-8345 (2018)
   PUBMED   30061385
REFERENCE   6  (bases 1 to 1184)
  AUTHORS   Fadeev,A., Mendoza-Garcia,P., Irion,U., Guan,J., Pfeifer,K.,
            Wiessner,S., Serluca,F., Singh,A.P., Nusslein-Volhard,C. and
            Palmer,R.H.
  TITLE     ALKALs are in vivo ligands for ALK family receptor tyrosine kinases
            in the neural crest and derived cells
  JOURNAL   Proc Natl Acad Sci U S A 115 (4), E630-E638 (2018)
   PUBMED   29317532
  REMARK    GeneRIF: activation of ALK/LTK family receptors by small ALKAL
            proteins (FAM150, AUG) conserved in vertebrates
REFERENCE   7  (bases 1 to 1184)
  AUTHORS   Reshetnyak,A.V., Murray,P.B., Shi,X., Mo,E.S., Mohanty,J., Tome,F.,
            Bai,H., Gunel,M., Lax,I. and Schlessinger,J.
  TITLE     Augmentor alpha and beta (FAM150) are ligands of the receptor
            tyrosine kinases ALK and LTK: Hierarchy and specificity of
            ligand-receptor interactions
  JOURNAL   Proc Natl Acad Sci U S A 112 (52), 15862-15867 (2015)
   PUBMED   26630010
  REMARK    GeneRIF: Data show FAM150B, which was named as augmentor-alpha
            (AUG-alpha), binds and activtes both anaplastic lymphoma kinase
            (ALK) and tyrosine kinase (LTK).
REFERENCE   8  (bases 1 to 1184)
  AUTHORS   Guan,J., Umapathy,G., Yamazaki,Y., Wolfstetter,G., Mendoza,P.,
            Pfeifer,K., Mohammed,A., Hugosson,F., Zhang,H., Hsu,A.W.,
            Halenbeck,R., Hallberg,B. and Palmer,R.H.
  TITLE     FAM150A and FAM150B are activating ligands for anaplastic lymphoma
            kinase
  JOURNAL   Elife 4, e09811 (2015)
   PUBMED   26418745
  REMARK    GeneRIF: In conclusion, these data show that ALK is robustly
            activated by the FAM150A/B ligands.
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 1184)
  AUTHORS   Zhang,H., Pao,L.I., Zhou,A., Brace,A.D., Halenbeck,R., Hsu,A.W.,
            Bray,T.L., Hestir,K., Bosch,E., Lee,E., Wang,G., Liu,H., Wong,B.R.,
            Kavanaugh,W.M. and Williams,L.T.
  TITLE     Deorphanization of the human leukocyte tyrosine kinase (LTK)
            receptor by a signaling screen of the extracellular proteome
  JOURNAL   Proc Natl Acad Sci U S A 111 (44), 15741-15745 (2014)
   PUBMED   25331893
  REMARK    GeneRIF: Only two related secreted factors, FAM150A and FAM150B
            (family with sequence similarity 150 member A and member B),
            stimulated LTK phosphorylation.
REFERENCE   10 (bases 1 to 1184)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC033490.1, AI300876.1 and AC079779.7.
            
            On Nov 22, 2018 this sequence version replaced NM_001002919.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC033490.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000403610.9/ ENSP00000384604.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1168              BC033490.1         92-1259
            1169-1181           AI300876.1         6-18                c
            1182-1184           AC079779.7         90729-90731         c
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p25.3"
     gene            1..1184
                     /gene="ALKAL2"
                     /gene_synonym="AUGA; FAM150B; PRO1097; RGPG542"
                     /note="ALK and LTK ligand 2"
                     /db_xref="GeneID:285016"
                     /db_xref="HGNC:HGNC:27683"
                     /db_xref="MIM:619671"
     exon            1..79
                     /gene="ALKAL2"
                     /gene_synonym="AUGA; FAM150B; PRO1097; RGPG542"
                     /inference="alignment:Splign:2.1.0"
     exon            80..389
                     /gene="ALKAL2"
                     /gene_synonym="AUGA; FAM150B; PRO1097; RGPG542"
                     /inference="alignment:Splign:2.1.0"
     CDS             137..595
                     /gene="ALKAL2"
                     /gene_synonym="AUGA; FAM150B; PRO1097; RGPG542"
                     /note="protein FAM150B; AUG-alpha; family with sequence
                     similarity 150 member B; augmentor alpha; augmentor-beta"
                     /codon_start=1
                     /product="ALK and LTK ligand 2 precursor"
                     /protein_id="NP_001002919.2"
                     /db_xref="CCDS:CCDS46218.1"
                     /db_xref="GeneID:285016"
                     /db_xref="HGNC:HGNC:27683"
                     /db_xref="MIM:619671"
                     /translation="MRGPGHPLLLGLLLVLGAAGRGRGGAEPREPADGQALLRLVVEL
                     VQELRKHHSAEHKGLQLLGRDCALGRAEAAGLGPSPEQRVEIVPRDLRMKDKFLKHLT
                     GPLYFSPKCSKHFHRLYHNTRDCTIPAYYKRCARLLTRLAVSPVCMEDKQ"
     exon            390..443
                     /gene="ALKAL2"
                     /gene_synonym="AUGA; FAM150B; PRO1097; RGPG542"
                     /inference="alignment:Splign:2.1.0"
     exon            444..524
                     /gene="ALKAL2"
                     /gene_synonym="AUGA; FAM150B; PRO1097; RGPG542"
                     /inference="alignment:Splign:2.1.0"
     exon            525..589
                     /gene="ALKAL2"
                     /gene_synonym="AUGA; FAM150B; PRO1097; RGPG542"
                     /inference="alignment:Splign:2.1.0"
     exon            590..1184
                     /gene="ALKAL2"
                     /gene_synonym="AUGA; FAM150B; PRO1097; RGPG542"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1146..1151
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ALKAL2"
                     /gene_synonym="AUGA; FAM150B; PRO1097; RGPG542"
                     /note="hexamer: AATAAA"
     regulatory      1163..1168
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ALKAL2"
                     /gene_synonym="AUGA; FAM150B; PRO1097; RGPG542"
                     /note="hexamer: AATGAA"
     polyA_site      1168
                     /gene="ALKAL2"
                     /gene_synonym="AUGA; FAM150B; PRO1097; RGPG542"
                     /note="major polyA site"
     polyA_site      1184
                     /gene="ALKAL2"
                     /gene_synonym="AUGA; FAM150B; PRO1097; RGPG542"
ORIGIN      
        1 gtgggcggga cctcgggcgt gatcgtcgag gagaccgcgg ctccctcacc tgccggcttg
       61 ttcctcgggg tccgcggagc gagcccagct ctcggcgcgt gtcggagtct cccagccccg
      121 cggccccgag cgcacgatgc gcggacccgg gcaccccctc ctcctggggc tgctgctggt
      181 gctgggggcg gcggggcgcg gccggggggg cgcggagccc cgggagccgg cggacggaca
      241 ggcgctgctg cggctggtgg tggaactcgt ccaggagctg cggaagcacc actcggcgga
      301 gcacaagggc ctgcagctcc tcgggcggga ctgcgccctg ggccgcgcgg aggcggcggg
      361 gctggggcct tcgccggagc agcgagtgga aattgttcct cgagatctga ggatgaagga
      421 caagtttcta aaacacctta caggccctct ttattttagt ccaaagtgca gcaaacactt
      481 ccatagactt tatcacaaca ccagagactg caccattcct gcatactata aaagatgcgc
      541 caggcttctt acccggctgg ctgtcagtcc agtgtgcatg gaggataagc agtgagcaga
      601 ccgtacagga gcagcacacc aggagccatg agaagtgcct tggaaaccaa cagggaaaca
      661 gaactatctt tatacacatc ccctcatgga caagagattt atttttgcag acagactctt
      721 ccataagtcc tttgagtttt gtatgttgtt gacagtttgc agatatatat tcgataaatc
      781 agtgtacttg acagtgttat ctgtcactta tttaaaaaaa aaacacaaaa ggaatgctcc
      841 acatttgacg tgtagtgcta taaaacacag aatatttcat tgtcttcatt aggtgaaatc
      901 gcaaaaaata tttctttaga aacataagca gaatcttaaa gtatattttc atataacata
      961 atttgatatt ctgtattact ttcactgtta aattctcaga gtattatttg gaacggcatg
     1021 aaaaattaaa atttcggtca tgttttagag acagtggagt gtaaatctgt ggctaattct
     1081 gttggtcgtt tgtattataa atgtaaaata gtattccagc tattgtgcaa tatgtaaata
     1141 gtgtaaataa acacaagtaa taaatgaagt gtttgttata atga
//
