LOCUS       NM_144988               9375 bp    mRNA    linear   PRI 09-OCT-2024
DEFINITION  Homo sapiens ALG14 UDP-N-acetylglucosaminyltransferase subunit
            (ALG14), transcript variant 1, mRNA.
ACCESSION   NM_144988
VERSION     NM_144988.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9375)
  AUTHORS   Wang,C.D., Xu,S., Chen,S., Chen,Z.H., Dean,N., Wang,N. and Gao,X.D.
  TITLE     An in vitro assay for enzymatic studies on human ALG13/14
            heterodimeric UDP-N-acetylglucosamine transferase
  JOURNAL   Front Cell Dev Biol 10, 1008078 (2022)
   PUBMED   36200043
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9375)
  AUTHORS   Palombo,F., Piccolo,B., Saccani,E., Fiorini,C., Capristo,M.,
            Caporali,L., Pisani,F. and Carelli,V.
  TITLE     A novel ALG14 missense variant in an alive child with myopathy,
            epilepsy, and progressive cerebral atrophy
  JOURNAL   Am J Med Genet A 185 (6), 1918-1921 (2021)
   PUBMED   33751823
  REMARK    GeneRIF: A novel ALG14 missense variant in an alive child with
            myopathy, epilepsy, and progressive cerebral atrophy.
REFERENCE   3  (bases 1 to 9375)
  AUTHORS   Kvarnung,M., Taylan,F., Nilsson,D., Anderlid,B.M., Malmgren,H.,
            Lagerstedt-Robinson,K., Holmberg,E., Burstedt,M., Nordenskjold,M.,
            Nordgren,A. and Lundberg,E.S.
  TITLE     Genomic screening in rare disorders: New mutations and phenotypes,
            highlighting ALG14 as a novel cause of severe intellectual
            disability
  JOURNAL   Clin Genet 94 (6), 528-537 (2018)
   PUBMED   30221345
  REMARK    GeneRIF: We highlight the findings in two affected siblings with
            splice altering variants in ALG14 and propose a new clinical
            entity, which includes severe intellectual disability, epilepsy,
            behavioral problems and mild dysmorphic features, caused by
            biallelic variants in ALG14.
REFERENCE   4  (bases 1 to 9375)
  AUTHORS   Schorling,D.C., Rost,S., Lefeber,D.J., Brady,L., Muller,C.R.,
            Korinthenberg,R., Tarnopolsky,M., Bonnemann,C.G., Rodenburg,R.J.,
            Bugiani,M., Beytia,M., Kruger,M., van der Knaap,M. and Kirschner,J.
  TITLE     Early and lethal neurodegeneration with myasthenic and myopathic
            features: A new ALG14-CDG
  JOURNAL   Neurology 89 (7), 657-664 (2017)
   PUBMED   28733338
  REMARK    GeneRIF: New ALG14 congenital disorder of glycosylation with early
            and lethal neurodegeneration with myasthenic and myopathic
            features.
REFERENCE   5  (bases 1 to 9375)
  AUTHORS   Cossins,J., Belaya,K., Hicks,D., Salih,M.A., Finlayson,S.,
            Carboni,N., Liu,W.W., Maxwell,S., Zoltowska,K., Farsani,G.T.,
            Laval,S., Seidhamed,M.Z., Donnelly,P., Bentley,D., McGowan,S.J.,
            Muller,J., Palace,J., Lochmuller,H. and Beeson,D.
  CONSRTM   WGS500 Consortium
  TITLE     Congenital myasthenic syndromes due to mutations in ALG2 and ALG14
  JOURNAL   Brain 136 (Pt 3), 944-956 (2013)
   PUBMED   23404334
  REMARK    GeneRIF: We identify ALG14 and ALG2 as novel genes in which
            mutations cause a congenital myasthenic syndrome
REFERENCE   6  (bases 1 to 9375)
  AUTHORS   Averbeck,N., Keppler-Ross,S. and Dean,N.
  TITLE     Membrane topology of the Alg14 endoplasmic reticulum UDP-GlcNAc
            transferase subunit
  JOURNAL   J Biol Chem 282 (40), 29081-29088 (2007)
   PUBMED   17686769
REFERENCE   7  (bases 1 to 9375)
  AUTHORS   Gao,X.D., Tachikawa,H., Sato,T., Jigami,Y. and Dean,N.
  TITLE     Alg14 recruits Alg13 to the cytoplasmic face of the endoplasmic
            reticulum to form a novel bipartite UDP-N-acetylglucosamine
            transferase required for the second step of N-linked glycosylation
  JOURNAL   J Biol Chem 280 (43), 36254-36262 (2005)
   PUBMED   16100110
  REMARK    GeneRIF: ALG13 and ALG14 form a functional endoplasmic reticulum
            UDP-N-acetylglucosamine transferase
REFERENCE   8  (bases 1 to 9375)
  AUTHORS   Chantret,I., Dancourt,J., Barbat,A. and Moore,S.E.
  TITLE     Two proteins homologous to the N- and C-terminal domains of the
            bacterial glycosyltransferase Murg are required for the second step
            of dolichyl-linked oligosaccharide synthesis in Saccharomyces
            cerevisiae
  JOURNAL   J Biol Chem 280 (10), 9236-9242 (2005)
   PUBMED   15615718
  REMARK    Erratum:[J Biol Chem. 2005 May 6;280(18):18551]
REFERENCE   9  (bases 1 to 9375)
  AUTHORS   Abicht,A., Muller,J.S. and Lochmuller,H.
  TITLE     Congenital Myasthenic Syndromes Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301347
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK289395.1 and AC105942.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_144988.3.
            
            Summary: This gene is a member of the glycosyltransferase 1 family.
            The encoded protein and ALG13 are thought to be subunits of
            UDP-GlcNAc transferase, which catalyzes the first two committed
            steps in endoplasmic reticulum N-linked glycosylation. Mutations in
            this gene have been linked to congenital myasthenic syndrome
            (CMSWTA). Alternatively spliced transcript variants have been
            identified. [provided by RefSeq, Mar 2015].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK289395.1, SRR3476690.303093.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2153932 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000370205.6/ ENSP00000359224.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1057              AK289395.1         1-1057
            1058-9375           AC105942.2         48416-56733
FEATURES             Location/Qualifiers
     source          1..9375
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p21.3"
     gene            1..9375
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
                     /note="ALG14 UDP-N-acetylglucosaminyltransferase subunit"
                     /db_xref="GeneID:199857"
                     /db_xref="HGNC:HGNC:28287"
                     /db_xref="MIM:612866"
     exon            1..189
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
                     /inference="alignment:Splign:2.1.0"
     CDS             54..704
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
                     /note="isoform 1 is encoded by transcript variant 1;
                     UDP-N-acetylglucosamine transferase subunit ALG14 homolog"
                     /codon_start=1
                     /product="UDP-N-acetylglucosamine transferase subunit
                     ALG14 isoform 1"
                     /protein_id="NP_659425.1"
                     /db_xref="CCDS:CCDS752.1"
                     /db_xref="GeneID:199857"
                     /db_xref="HGNC:HGNC:28287"
                     /db_xref="MIM:612866"
                     /translation="MVCVLVLAAAAGAVAVFLILRIWVVLRSMDVTPRESLSILVVAG
                     SGGHTTEILRLLGSLSNAYSPRHYVIADTDEMSANKINSFELDRADRDPSNMYTKYYI
                     HRIPRSREVQQSWPSTVFTTLHSMWLSFPLIHRVKPDLVLCNGPGTCVPICVSALLLG
                     ILGIKKVIIVYVESICRVETLSMSGKILFHLSDYFIVQWPALKEKYPKSVYLGRIV"
     exon            190..341
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
                     /inference="alignment:Splign:2.1.0"
     exon            342..473
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
                     /inference="alignment:Splign:2.1.0"
     exon            474..9375
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      945..950
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
                     /note="hexamer: GATAAA"
     polyA_site      972
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
                     /note="major polyA site"
     regulatory      1015..1020
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
                     /note="hexamer: AATATA"
     polyA_site      1041
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
     regulatory      9351..9356
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
                     /note="hexamer: AATAAA"
     polyA_site      9375
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
ORIGIN      
        1 aactttgaca gcggctggtc cccggaagtt ggacgcatgc gccgtttctc tgcatggtgt
       61 gcgttctcgt tctagctgcg gccgcaggag ctgtggcggt tttcctaatc ctgcgaatat
      121 gggtagtgct tcgttccatg gacgttacgc cccgggagtc tctcagtatc ttggtagtgg
      181 ctgggtccgg tgggcatacc actgagatcc tgaggctgct tgggagcttg tccaatgcct
      241 actcacctag acattatgtc attgctgaca ctgatgaaat gagtgccaat aaaataaatt
      301 cttttgaact agatcgagct gatagagacc ctagtaacat gtataccaaa tactacattc
      361 accgaattcc aagaagccgg gaggttcagc agtcctggcc ctccaccgtt ttcaccacct
      421 tgcactccat gtggctctcc tttcccctaa ttcacagggt gaagccagat ttggtgttgt
      481 gtaacggacc aggaacatgt gttcctatct gtgtatctgc ccttctcctt gggatactag
      541 gaataaagaa agtgatcatt gtctacgttg aaagcatctg ccgtgtagaa acgttatcca
      601 tgtccggaaa gattctgttt catctctcag attacttcat tgttcagtgg ccggctctga
      661 aagaaaagta tcccaaatcg gtgtaccttg ggcgaattgt ttgacaaatg gcaactgact
      721 tctttagaat tttgcagtta acagtagtat gtactcaaat tggggggaaa aaaaccctac
      781 atgtttcttg taaaggcgtc tgacagtcct gagaattatt gatggtaagg aataaaaaat
      841 gtacagatga ctcagtgaag aaactgaggc ttctcttatg aaacaaacat tgataaacgt
      901 aactactaaa tgtttatgcc tctgtaaacc aaatttcttt tctagataaa aatatgtatt
      961 actacctgca aattttcttc tggctgtttt agtagtattt tttttacaga actaaatata
     1021 gagtttgtat gattagtaat atttcctgtc ctacagcttt tttttttttt tttttttttt
     1081 gtaaagtatt ctgcattgta aaagaagaga gaaaaaaagt atttctgagt tttggttcag
     1141 atacaaaagg ctttatttcc tgtatggaaa cagtgatgtc cccagtgaat cctttcagca
     1201 taaaggaagc agttatatta aattggcttc atgacaattt gcactccaca agcctccaga
     1261 accttgagca taaaccagta tcattttttg tacttcagtc ttgctttttg agtagttggc
     1321 cttaatacag agtatctttt aaaatctctt gtttaggccg gcatgacggc tcacacctgt
     1381 aatcccagca ccttgggagg ctaaggcggg tggatcactg gaggtcagga gtttgacacc
     1441 agcctgacca acatggtgaa accttgtctc tactaaaaat acaaaaatta gtcaggcgtg
     1501 gtggcacatg cctgtaatcc cagctactcg ggaggctgag gcaggagaat cgcttgaacc
     1561 cgggaggcag aggctgcagt gagccgagat tgcaccattg cactccatcc agcctgggtg
     1621 acaagagtga aacttcgtct caaaaaaaaa aaaaaaaaac aaaactcaat gatgtaggga
     1681 ctgttattat tcccatttta cagatgactt gtccaagatc acatgagtag taagtagcaa
     1741 agccagaatt caagttcagg taaacctgac ttcttagtgg ggccagtttc acgggtgtgt
     1801 ggcctgtgca gtcacacaga gttctgcact gaagagggcc tccacttggt ttaatgttct
     1861 gctgtgactc tcttgaactt ctgaatattt ttttaaacaa tgaacttata ttttagaccc
     1921 tctgaattct ttcgctggtc ctgcttgaga ctgtgtttct agtggtgcca tgtctactgg
     1981 aatagaagta caatagttag tgcagtgcct ggcccaggag ctcctcaaaa acaggaattc
     2041 catttccttc cctctaacct ctgcttcttt aacaaagcag ccaaaaaaaa aaaaaacaaa
     2101 acaaaataaa aaacagaaaa gagaagattc tctcctgtag tatatatagg ttttttcctc
     2161 ttaaatcttt tgctttataa gtatgatata aatctaataa atagtgatta gtgaagtgat
     2221 tgaatttttg tgaaaagaga catgattatc cacgatcatg taagattaaa atcctgaggc
     2281 tgatccagcc ttttttcttt tttttttttt taaaaaaaaa aagcaaaaaa aacaaaaaaa
     2341 ctgtcttctc tgaatcatgt aacaggcgca tctgtcagcc ggacatcttc ctaggttttt
     2401 ggctggagaa atgtgtagcc gaaggtgtct cagaatggtg agaacacttt gctgtctgga
     2461 tttagtgggc ctggggcagc caaatgatag ttcattgtgc tttgatttta atcctcaaat
     2521 ctcagtcact ggttgcttcc ctggagtatc agagcgtatt agtatcatac aaccaggggc
     2581 tgaggtgaaa taagccttga ctgttctaga aagaaagtga ttttttcagg gacaacagca
     2641 tctgcatttt attgctgttt aaggctttct ctgctctgta ctttgttccc tgagtggcac
     2701 taggtaaatg aatggtaagg gaaagtgaac tgcgtttagt gaatgcctct ctgtgctgtg
     2761 gggcagcttc cctgggcccc cacaggtaat cctgctgcag aggcttctgt caggtcagag
     2821 cttaacaaag atcagaccag agagaattgt agaaaagatg ctataatttt ttccagaaac
     2881 atatgtccca gatgttttgc agagccttaa cttaagacag tggttagcct ggaatgtcag
     2941 agaaagaatg tgtatgtgtt atatcaccaa aagtttagaa atgttatatc tttcataggt
     3001 tcaggctgct gtgtactcat tcagagccag gctgagacat gtgttattgc atcttcctga
     3061 tcattcagat tactctggta aacaagtgca tgctctgctg tgtgttactg ttatggctaa
     3121 tttgagtcct caaggtccca ttagcagagg gggctggtgg cacaatttcc ctgctgtgca
     3181 tttctgtgac attcagaaag atagtgcgcc agtcccaact caaactgaag aagatgagta
     3241 gctagcttgt cccttccagt ttctcttaga acactctgaa tgcaactttt actgtaggag
     3301 actgttcatg aaaaaattaa ttgaatcagt cttcaaatga gaggcaggga aaccacacat
     3361 cctgcccaca cctacttccc tggaagattt ctatctacag tccagctatc ctaaaaggtc
     3421 tcagtagctt gccaggtcag aatgtctaac tgtttcaaaa aggatgccca aaaatcatga
     3481 caggaaactt tagcctggaa cgaagagtgc tatgaaaccg tgtcaacaag aggagtggtg
     3541 acaattgtga gttgaatagc aagatgtcct tgctaggaag gagatgaccc aagcttcccc
     3601 ttataaaagc aacttggaac acatcattct acctttcaaa gatacatggt aagttaaaat
     3661 tgagaaacgt acccctttac tatacagttc cttgggttga atgagtgctt tttttttttt
     3721 ctgaaaagcc ccatccccca aaaggcattt gatagcaatg tattaagcac cttttaccaa
     3781 caataagaac atcattgtaa gtgttaaaga aaattgtcct tctccctaca ggtcagcgtt
     3841 tgagcctcac ggggtttctt cccatgactt cagccctggc gcacaatgag cagacaccca
     3901 aatcttgtcc gccttcaaca ttcttttgtt agggcccatc cctctagcac tctaaaaaca
     3961 aatcatctac aactgtgttc ctgctgccca ctcattttaa acatgaattt gacatagctc
     4021 ctccttttcg cactggagta gttaactaat gctagcccac tagcttctat catgtccctc
     4081 aagcaacaaa ctttgggatc atttttgctt tttcactctt tctttaccca attataattg
     4141 gttgcctttt ttgtttcatt atccccagaa ttttctcgtc ttcctcttct ctccttgaaa
     4201 ttacttttac tgttcctctc ctttgtcatc acctattgat tctgcctttg acttagttca
     4261 tatctaccct ttccttttca gtcccatagt tacaacctaa gccagctctt caacaaaaat
     4321 ttcttgcttg atgagcaagt tttattgagg aactgaggaa acacctgtgg aaggaacaaa
     4381 tgaatgattt tgttaattac ttatgtttgt cttcagagac ttcccctcat gctagttctg
     4441 tatacttgtt tcactttttt ctttcttttt tttttttttt tttttttttc gagacagtct
     4501 cgctccatcg cccaggctgg agtgcagtgg tgcaatctca ccttactggc tcactgtaac
     4561 ctctgcctcc tggcttcaag cgattcttgt gcctcagcct cccgagtagc tgggattaca
     4621 ggcatgcacc accacacctg gctaattttt gtatttttag tagagacagg gttttaccat
     4681 gttggccagg ctgatcttga actcccaacc tcaggtgatc cacccatctt ggcctcccaa
     4741 agtgctggaa ttacaggtgt aagccacacg tccggccctt gttccacttt ttataatatt
     4801 tacaccacag agaggttaag taatttatta aagattatag aactagaaag tgacaaagct
     4861 cagatttgaa ccaaggcatt ctagctccag ccaggcttac cttaatagct acgttaggtg
     4921 tttttttttt tttttaaaga aaaaagaaaa aatgtcctca aaaggaaata aatggggtaa
     4981 cagggtacta tgacggagta gaaggtagtt ggtgttctag atagggaaat caggaaaggc
     5041 ctttctaaga aagtggtatt ttacctgagt tccaaaggat aatgccttaa atatttgtcc
     5101 tccttctact tcactcccaa cttagatttt gccccaccca caaagtcttt gtcttcattt
     5161 agcttgagca aattctctaa ataactctgt gacatagcca cccataccaa aggttagtgc
     5221 tgagttatat accatcgaat tgcagtcctt gttcttagaa acacctgtgg aaggaataat
     5281 gaatgctatt gctgattatt tatgtttgtc ttcagggact gtacctcatt ctagttttgt
     5341 atccttgttc cacttttgat agtatttaca ccacagctct gtacaaaaca taggcactaa
     5401 ttaaatacat aaattatttg gccaggcatg gtggctcacg cctgtaatcc cagcactttg
     5461 ggaggccaag gtgggtgtat cacaaggtca ggagttcaag accagcctgg ccaagatggt
     5521 gaaaccccat ctctactaaa aatacaaaaa atttgccggc gtggtggcgg gcgcctgtaa
     5581 ttccagctac ttgggaggct gaggcaaata attgcttgaa ccagggaggt ggaggttgca
     5641 gtgaactgag atggcaccac tgcactccag cctgggggac agagcgagac tccacctcaa
     5701 aaaaaaaaaa agaaaaaaat tatagcttca gacttgtcta ggctcacttc agttatattt
     5761 tatctagacc agtgcttcac aaacgttttg atctcaggac ctcattatac taaaaaatta
     5821 ttgaaaactg gctgggcaca gtgtttcaca cttgtaatcc cagcactttg ggaggccgag
     5881 gtggatggat cacctgagcc caggagtttg agatcagcct gggcaacaca gtaaaacccc
     5941 atctctacaa aaaaaataca aaaattagcc aggcatggtg atgcacacct atagttccag
     6001 ctacttggga ggctgaggtg gtaggatcac ttgagcctgg gaggtggagg ttgcagtgag
     6061 ccgagatcat ggcactgcac tccagcctgg gtgacagagt gagaccctgc ctcagaaaaa
     6121 aaaaaaatat atcccaatga gcttttgttt gtgtgagcta tatttgtcaa tatttaccat
     6181 tgtagaaact agacaaaaat tttaaacgca agaatataaa aggacatatt ccactggctg
     6241 tcagaacaat atcatcacac atcttgtagc atctggaaaa ctataccatc ataagaatga
     6301 caaggaaaag gcaaataaca tcttaatatt actatgaaaa tagttttgac ctcataaacc
     6361 cctaaaaacg tcttgaagac tccccaggac cttggaccac attttaagaa ctgctgcttt
     6421 acataatgtc acatcagcga attcatctct tcttttttta aaataagagg cagggtttca
     6481 ctatgttgtc cagactggtc tcaaactcct ggtctcaagg aatcctccta tcttggcctt
     6541 ccaaagtgct ggaatcacag gcatgcggca ctgcgcccgg cctatctcct ctttagtcag
     6601 tcagtgatct gtgtattagt tttatcgctg tgtaaaaaat tgccacacat atattggctt
     6661 aaaacaatac attatctctg taggttagaa atccaggcaa agaaggtttt ctgctcaaga
     6721 tctcaaaagg ctgaaatcaa gatgatggcc aaggctgagt tctgatctgc aactctgaat
     6781 ccttttcaga taccttcaga tggttgttgg cagaattcag ttccttgcag ttgtaggatt
     6841 tgtggtggcc tgcttcctat cttttaagga ctcacatgac tgagtcagtg taatctccct
     6901 tttgattaat tcaaggtcaa ctgattagga tcataattac gtcccttttg ccatgttaat
     6961 gtaacataat cagaggaatg acatgtcatg atattcacag atcccaccca tactaacgga
     7021 tagtagatta tccagggcat gtaaatcagg gggtggaata ttaggggaca tctttttttt
     7081 ttttctttaa gacggagtct cactctgtca cccaggctgg agtgctgtgg tgcaatctca
     7141 actcactgca acctctacct ccctggttca agcgattctc gtgcctcagc cgcctgacta
     7201 gctgggattt caggagtgca ccaccagccc ggctaacttt tgtattttta gcagagatgg
     7261 ggtttcacca tgttggccgg gctggtctcg aactcctggg ctcaagtgat ccgcccacct
     7321 cggcttccca aagtgctgga attacaggcg tcagccacca tgcccgttct gggggccatc
     7381 tgagacttct gccaaccaca atcagctaga tgttttttgt tttgttttga aaaaataata
     7441 ataataacct ttcacttggg ccaccaaact cctaccttag tatccaagtt ctctaatttt
     7501 tttttaatat gggaaatcca catctcccaa actctatccc ttttctggca agttacttga
     7561 cctttctgta aaataagcat aatgtacctg cgttagagtt gtagggagca ctaaacattc
     7621 agatgatgta tgtacagtgt ttagcacaga gcctgctaaa tagtaagccc tcaagccctc
     7681 agtcacattt gtctccatca gtggtagctc ccctggtgtg ttaaggaggc tcttctttct
     7741 tttttttttt tttttttttt tttgagacag agtctcgctc tgttgcccag gctgaagtgc
     7801 agtggcgcga tctcggctca ctgcaagctc cgcctcctgg gttcacgcca ttgtcctgcc
     7861 tcagcctccc gagtagctgg gattacaggc gcctgtcacc acgcccggct aatttttttt
     7921 ttgtttgttt tttttgtatt tttagtagag acagggtttc actgtgttag ccaggatggt
     7981 ctccatctcc tcacctcgtg atctgcccgc ctcggcctcc caaagtgctg ggattacagg
     8041 cgtgagccac tgcgcccggc caggaggctc tttttaaact aagggcagca accccctagg
     8101 gtggctatag tccaagaaaa cccagaaaat acatgctgcc aaggatttgg agaaattgga
     8161 gccctcctac attgctggta ggaatgtaga atggtgcagc agctgtggaa aacagttcag
     8221 cagtttcaca aaaatcaaac atagggttat catatgaccc agaaattcta cccataggtg
     8281 tataccctgg agaattaaaa acatgtattt acacaaaaac ttatatgttc gcagcagcgt
     8341 tattcataat aacccgaaag tagaaacaac ccaaatgctc tactaatgaa cagataaaca
     8401 aaatgtggta tatccatata gtggaatatt actcgatcac acaaaggaat gaagtactaa
     8461 tacatgctac agtgtggatg aaccttgtaa acagtaggct aagttacaga agccagccac
     8521 aaaagacaac aaatgcatga ttctattcat atgaaatgtc cagagtaggc aagtccatag
     8581 acagaaagta gcttagtggt ttccaggggc agtgagaagg ggaaatggag ggcaactcct
     8641 aatgggtatg aggcttcttt tggaggtgat gaaaatgttc tggaattcga cagtggtgat
     8701 ggttgtacat ctctgtgaat atactaaaag ccactgaatt gtatactggg tgaattttat
     8761 ggtatctgta gacattccaa ttgacagtca catcttagta tagactttga gtcatctcac
     8821 tggaggcact agacatgcaa gaggagaagt ctccagatta ttccagccct gccatttgag
     8881 tcactccagg ctttctggtc ttcccagctg aggctcagac accttggaag agagaaaagc
     8941 tctcccttct gtgccctgtg atttgctgat ccacagagtt cataggcatg attaaatggt
     9001 agtagttttc caccacgaag ttttggaata atttgtcaca aagcaatagg taattagaaa
     9061 aataaccttt cagaaggaga cagtttctcc aaagcttggg tactttcgct taatattaaa
     9121 agaggaagaa attttttgcc tgccaggcaa aaggcagaag atgtatttct acaccatgga
     9181 taggaaaatg attttttttc ctcctttctc cttggcctgc atgcagcagt gaaaaagaaa
     9241 ccaatggatt cttttaaatc tttaaccata tgcagatgaa taagcacatg gttggacttg
     9301 attcctaatc tcttttcctt tcctgtataa ctgagatttt tttccactct aataaacttg
     9361 agcatttaaa cagaa
//
