LOCUS       NM_001305242            9412 bp    mRNA    linear   PRI 11-OCT-2024
DEFINITION  Homo sapiens ALG14 UDP-N-acetylglucosaminyltransferase subunit
            (ALG14), transcript variant 2, mRNA.
ACCESSION   NM_001305242
VERSION     NM_001305242.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Wang,C.D., Xu,S., Chen,S., Chen,Z.H., Dean,N., Wang,N. and Gao,X.D.
  TITLE     An in vitro assay for enzymatic studies on human ALG13/14
            heterodimeric UDP-N-acetylglucosamine transferase
  JOURNAL   Front Cell Dev Biol 10, 1008078 (2022)
   PUBMED   36200043
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   Palombo,F., Piccolo,B., Saccani,E., Fiorini,C., Capristo,M.,
            Caporali,L., Pisani,F. and Carelli,V.
  TITLE     A novel ALG14 missense variant in an alive child with myopathy,
            epilepsy, and progressive cerebral atrophy
  JOURNAL   Am J Med Genet A 185 (6), 1918-1921 (2021)
   PUBMED   33751823
  REMARK    GeneRIF: A novel ALG14 missense variant in an alive child with
            myopathy, epilepsy, and progressive cerebral atrophy.
REFERENCE   3  (bases 1 to 9412)
  AUTHORS   Kvarnung,M., Taylan,F., Nilsson,D., Anderlid,B.M., Malmgren,H.,
            Lagerstedt-Robinson,K., Holmberg,E., Burstedt,M., Nordenskjold,M.,
            Nordgren,A. and Lundberg,E.S.
  TITLE     Genomic screening in rare disorders: New mutations and phenotypes,
            highlighting ALG14 as a novel cause of severe intellectual
            disability
  JOURNAL   Clin Genet 94 (6), 528-537 (2018)
   PUBMED   30221345
  REMARK    GeneRIF: We highlight the findings in two affected siblings with
            splice altering variants in ALG14 and propose a new clinical
            entity, which includes severe intellectual disability, epilepsy,
            behavioral problems and mild dysmorphic features, caused by
            biallelic variants in ALG14.
REFERENCE   4  (bases 1 to 9412)
  AUTHORS   Schorling,D.C., Rost,S., Lefeber,D.J., Brady,L., Muller,C.R.,
            Korinthenberg,R., Tarnopolsky,M., Bonnemann,C.G., Rodenburg,R.J.,
            Bugiani,M., Beytia,M., Kruger,M., van der Knaap,M. and Kirschner,J.
  TITLE     Early and lethal neurodegeneration with myasthenic and myopathic
            features: A new ALG14-CDG
  JOURNAL   Neurology 89 (7), 657-664 (2017)
   PUBMED   28733338
  REMARK    GeneRIF: New ALG14 congenital disorder of glycosylation with early
            and lethal neurodegeneration with myasthenic and myopathic
            features.
REFERENCE   5  (bases 1 to 9412)
  AUTHORS   Cossins,J., Belaya,K., Hicks,D., Salih,M.A., Finlayson,S.,
            Carboni,N., Liu,W.W., Maxwell,S., Zoltowska,K., Farsani,G.T.,
            Laval,S., Seidhamed,M.Z., Donnelly,P., Bentley,D., McGowan,S.J.,
            Muller,J., Palace,J., Lochmuller,H. and Beeson,D.
  CONSRTM   WGS500 Consortium
  TITLE     Congenital myasthenic syndromes due to mutations in ALG2 and ALG14
  JOURNAL   Brain 136 (Pt 3), 944-956 (2013)
   PUBMED   23404334
  REMARK    GeneRIF: We identify ALG14 and ALG2 as novel genes in which
            mutations cause a congenital myasthenic syndrome
REFERENCE   6  (bases 1 to 9412)
  AUTHORS   Averbeck,N., Keppler-Ross,S. and Dean,N.
  TITLE     Membrane topology of the Alg14 endoplasmic reticulum UDP-GlcNAc
            transferase subunit
  JOURNAL   J Biol Chem 282 (40), 29081-29088 (2007)
   PUBMED   17686769
REFERENCE   7  (bases 1 to 9412)
  AUTHORS   Gao,X.D., Tachikawa,H., Sato,T., Jigami,Y. and Dean,N.
  TITLE     Alg14 recruits Alg13 to the cytoplasmic face of the endoplasmic
            reticulum to form a novel bipartite UDP-N-acetylglucosamine
            transferase required for the second step of N-linked glycosylation
  JOURNAL   J Biol Chem 280 (43), 36254-36262 (2005)
   PUBMED   16100110
  REMARK    GeneRIF: ALG13 and ALG14 form a functional endoplasmic reticulum
            UDP-N-acetylglucosamine transferase
REFERENCE   8  (bases 1 to 9412)
  AUTHORS   Chantret,I., Dancourt,J., Barbat,A. and Moore,S.E.
  TITLE     Two proteins homologous to the N- and C-terminal domains of the
            bacterial glycosyltransferase Murg are required for the second step
            of dolichyl-linked oligosaccharide synthesis in Saccharomyces
            cerevisiae
  JOURNAL   J Biol Chem 280 (10), 9236-9242 (2005)
   PUBMED   15615718
  REMARK    Erratum:[J Biol Chem. 2005 May 6;280(18):18551]
REFERENCE   9  (bases 1 to 9412)
  AUTHORS   Abicht,A., Muller,J.S. and Lochmuller,H.
  TITLE     Congenital Myasthenic Syndromes Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301347
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK289395.1, AW242346.1 and
            AC105942.2.
            
            On May 31, 2019 this sequence version replaced NM_001305242.1.
            
            Summary: This gene is a member of the glycosyltransferase 1 family.
            The encoded protein and ALG13 are thought to be subunits of
            UDP-GlcNAc transferase, which catalyzes the first two committed
            steps in endoplasmic reticulum N-linked glycosylation. Mutations in
            this gene have been linked to congenital myasthenic syndrome
            (CMSWTA). Alternatively spliced transcript variants have been
            identified. [provided by RefSeq, Mar 2015].
            
            Transcript Variant: This variant (2) contains an alternate exon in
            the central coding region, which results in a frameshift and an
            early stop codon, compared to variant 1. The encoded isoform (2)
            has a shorter and distinct C-terminus, compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AW242346.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2154361, SAMN03465405
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-134               AK289395.1         1-134
            135-475             AW242346.1         49-389
            476-1094            AK289395.1         439-1057
            1095-9412           AC105942.2         48416-56733
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p21.3"
     gene            1..9412
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
                     /note="ALG14 UDP-N-acetylglucosaminyltransferase subunit"
                     /db_xref="GeneID:199857"
                     /db_xref="HGNC:HGNC:28287"
                     /db_xref="MIM:612866"
     exon            1..189
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
                     /inference="alignment:Splign:2.1.0"
     CDS             54..521
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
                     /note="isoform 2 is encoded by transcript variant 2;
                     UDP-N-acetylglucosamine transferase subunit ALG14 homolog"
                     /codon_start=1
                     /product="UDP-N-acetylglucosamine transferase subunit
                     ALG14 isoform 2"
                     /protein_id="NP_001292171.1"
                     /db_xref="GeneID:199857"
                     /db_xref="HGNC:HGNC:28287"
                     /db_xref="MIM:612866"
                     /translation="MVCVLVLAAAAGAVAVFLILRIWVVLRSMDVTPRESLSILVVAG
                     SGGHTTEILRLLGSLSNAYSPRHYVIADTDEMSANKINSFELDRADRDPSNMDFSVVW
                     HSLNNKVYQILHSPNSKKPGGSAVLALHRFHHLALHVALLSPNSQGEARFGVV"
     exon            190..341
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
                     /inference="alignment:Splign:2.1.0"
     exon            342..378
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
                     /inference="alignment:Splign:2.1.0"
     exon            379..510
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
                     /inference="alignment:Splign:2.1.0"
     exon            511..9412
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      982..987
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
                     /note="hexamer: GATAAA"
     polyA_site      1009
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
                     /note="major polyA site"
     regulatory      1052..1057
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
                     /note="hexamer: AATATA"
     polyA_site      1078
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
     regulatory      9388..9393
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
                     /note="hexamer: AATAAA"
     polyA_site      9412
                     /gene="ALG14"
                     /gene_synonym="CMS15; IDDEBF; MEPCA"
ORIGIN      
        1 aactttgaca gcggctggtc cccggaagtt ggacgcatgc gccgtttctc tgcatggtgt
       61 gcgttctcgt tctagctgcg gccgcaggag ctgtggcggt tttcctaatc ctgcgaatat
      121 gggtagtgct tcgttccatg gacgttacgc cccgggagtc tctcagtatc ttggtagtgg
      181 ctgggtccgg tgggcatacc actgagatcc tgaggctgct tgggagcttg tccaatgcct
      241 actcacctag acattatgtc attgctgaca ctgatgaaat gagtgccaat aaaataaatt
      301 cttttgaact agatcgagct gatagagacc ctagtaacat ggacttttca gtcgtgtggc
      361 attcactaaa taataaagta taccaaatac tacattcacc gaattccaag aagccgggag
      421 gttcagcagt cctggccctc caccgttttc accaccttgc actccatgtg gctctccttt
      481 cccctaattc acagggtgaa gccagatttg gtgttgtgta acggaccagg aacatgtgtt
      541 cctatctgtg tatctgccct tctccttggg atactaggaa taaagaaagt gatcattgtc
      601 tacgttgaaa gcatctgccg tgtagaaacg ttatccatgt ccggaaagat tctgtttcat
      661 ctctcagatt acttcattgt tcagtggccg gctctgaaag aaaagtatcc caaatcggtg
      721 taccttgggc gaattgtttg acaaatggca actgacttct ttagaatttt gcagttaaca
      781 gtagtatgta ctcaaattgg ggggaaaaaa accctacatg tttcttgtaa aggcgtctga
      841 cagtcctgag aattattgat ggtaaggaat aaaaaatgta cagatgactc agtgaagaaa
      901 ctgaggcttc tcttatgaaa caaacattga taaacgtaac tactaaatgt ttatgcctct
      961 gtaaaccaaa tttcttttct agataaaaat atgtattact acctgcaaat tttcttctgg
     1021 ctgttttagt agtatttttt ttacagaact aaatatagag tttgtatgat tagtaatatt
     1081 tcctgtccta cagctttttt tttttttttt tttttttgta aagtattctg cattgtaaaa
     1141 gaagagagaa aaaaagtatt tctgagtttt ggttcagata caaaaggctt tatttcctgt
     1201 atggaaacag tgatgtcccc agtgaatcct ttcagcataa aggaagcagt tatattaaat
     1261 tggcttcatg acaatttgca ctccacaagc ctccagaacc ttgagcataa accagtatca
     1321 ttttttgtac ttcagtcttg ctttttgagt agttggcctt aatacagagt atcttttaaa
     1381 atctcttgtt taggccggca tgacggctca cacctgtaat cccagcacct tgggaggcta
     1441 aggcgggtgg atcactggag gtcaggagtt tgacaccagc ctgaccaaca tggtgaaacc
     1501 ttgtctctac taaaaataca aaaattagtc aggcgtggtg gcacatgcct gtaatcccag
     1561 ctactcggga ggctgaggca ggagaatcgc ttgaacccgg gaggcagagg ctgcagtgag
     1621 ccgagattgc accattgcac tccatccagc ctgggtgaca agagtgaaac ttcgtctcaa
     1681 aaaaaaaaaa aaaaaacaaa actcaatgat gtagggactg ttattattcc cattttacag
     1741 atgacttgtc caagatcaca tgagtagtaa gtagcaaagc cagaattcaa gttcaggtaa
     1801 acctgacttc ttagtggggc cagtttcacg ggtgtgtggc ctgtgcagtc acacagagtt
     1861 ctgcactgaa gagggcctcc acttggttta atgttctgct gtgactctct tgaacttctg
     1921 aatatttttt taaacaatga acttatattt tagaccctct gaattctttc gctggtcctg
     1981 cttgagactg tgtttctagt ggtgccatgt ctactggaat agaagtacaa tagttagtgc
     2041 agtgcctggc ccaggagctc ctcaaaaaca ggaattccat ttccttccct ctaacctctg
     2101 cttctttaac aaagcagcca aaaaaaaaaa aaacaaaaca aaataaaaaa cagaaaagag
     2161 aagattctct cctgtagtat atataggttt tttcctctta aatcttttgc tttataagta
     2221 tgatataaat ctaataaata gtgattagtg aagtgattga atttttgtga aaagagacat
     2281 gattatccac gatcatgtaa gattaaaatc ctgaggctga tccagccttt tttctttttt
     2341 ttttttttaa aaaaaaaaag caaaaaaaac aaaaaaactg tcttctctga atcatgtaac
     2401 aggcgcatct gtcagccgga catcttccta ggtttttggc tggagaaatg tgtagccgaa
     2461 ggtgtctcag aatggtgaga acactttgct gtctggattt agtgggcctg gggcagccaa
     2521 atgatagttc attgtgcttt gattttaatc ctcaaatctc agtcactggt tgcttccctg
     2581 gagtatcaga gcgtattagt atcatacaac caggggctga ggtgaaataa gccttgactg
     2641 ttctagaaag aaagtgattt tttcagggac aacagcatct gcattttatt gctgtttaag
     2701 gctttctctg ctctgtactt tgttccctga gtggcactag gtaaatgaat ggtaagggaa
     2761 agtgaactgc gtttagtgaa tgcctctctg tgctgtgggg cagcttccct gggcccccac
     2821 aggtaatcct gctgcagagg cttctgtcag gtcagagctt aacaaagatc agaccagaga
     2881 gaattgtaga aaagatgcta taattttttc cagaaacata tgtcccagat gttttgcaga
     2941 gccttaactt aagacagtgg ttagcctgga atgtcagaga aagaatgtgt atgtgttata
     3001 tcaccaaaag tttagaaatg ttatatcttt cataggttca ggctgctgtg tactcattca
     3061 gagccaggct gagacatgtg ttattgcatc ttcctgatca ttcagattac tctggtaaac
     3121 aagtgcatgc tctgctgtgt gttactgtta tggctaattt gagtcctcaa ggtcccatta
     3181 gcagaggggg ctggtggcac aatttccctg ctgtgcattt ctgtgacatt cagaaagata
     3241 gtgcgccagt cccaactcaa actgaagaag atgagtagct agcttgtccc ttccagtttc
     3301 tcttagaaca ctctgaatgc aacttttact gtaggagact gttcatgaaa aaattaattg
     3361 aatcagtctt caaatgagag gcagggaaac cacacatcct gcccacacct acttccctgg
     3421 aagatttcta tctacagtcc agctatccta aaaggtctca gtagcttgcc aggtcagaat
     3481 gtctaactgt ttcaaaaagg atgcccaaaa atcatgacag gaaactttag cctggaacga
     3541 agagtgctat gaaaccgtgt caacaagagg agtggtgaca attgtgagtt gaatagcaag
     3601 atgtccttgc taggaaggag atgacccaag cttcccctta taaaagcaac ttggaacaca
     3661 tcattctacc tttcaaagat acatggtaag ttaaaattga gaaacgtacc cctttactat
     3721 acagttcctt gggttgaatg agtgcttttt tttttttctg aaaagcccca tcccccaaaa
     3781 ggcatttgat agcaatgtat taagcacctt ttaccaacaa taagaacatc attgtaagtg
     3841 ttaaagaaaa ttgtccttct ccctacaggt cagcgtttga gcctcacggg gtttcttccc
     3901 atgacttcag ccctggcgca caatgagcag acacccaaat cttgtccgcc ttcaacattc
     3961 ttttgttagg gcccatccct ctagcactct aaaaacaaat catctacaac tgtgttcctg
     4021 ctgcccactc attttaaaca tgaatttgac atagctcctc cttttcgcac tggagtagtt
     4081 aactaatgct agcccactag cttctatcat gtccctcaag caacaaactt tgggatcatt
     4141 tttgcttttt cactctttct ttacccaatt ataattggtt gccttttttg tttcattatc
     4201 cccagaattt tctcgtcttc ctcttctctc cttgaaatta cttttactgt tcctctcctt
     4261 tgtcatcacc tattgattct gcctttgact tagttcatat ctaccctttc cttttcagtc
     4321 ccatagttac aacctaagcc agctcttcaa caaaaatttc ttgcttgatg agcaagtttt
     4381 attgaggaac tgaggaaaca cctgtggaag gaacaaatga atgattttgt taattactta
     4441 tgtttgtctt cagagacttc ccctcatgct agttctgtat acttgtttca cttttttctt
     4501 tctttttttt tttttttttt ttttttcgag acagtctcgc tccatcgccc aggctggagt
     4561 gcagtggtgc aatctcacct tactggctca ctgtaacctc tgcctcctgg cttcaagcga
     4621 ttcttgtgcc tcagcctccc gagtagctgg gattacaggc atgcaccacc acacctggct
     4681 aatttttgta tttttagtag agacagggtt ttaccatgtt ggccaggctg atcttgaact
     4741 cccaacctca ggtgatccac ccatcttggc ctcccaaagt gctggaatta caggtgtaag
     4801 ccacacgtcc ggcccttgtt ccacttttta taatatttac accacagaga ggttaagtaa
     4861 tttattaaag attatagaac tagaaagtga caaagctcag atttgaacca aggcattcta
     4921 gctccagcca ggcttacctt aatagctacg ttaggtgttt tttttttttt ttaaagaaaa
     4981 aagaaaaaat gtcctcaaaa ggaaataaat ggggtaacag ggtactatga cggagtagaa
     5041 ggtagttggt gttctagata gggaaatcag gaaaggcctt tctaagaaag tggtatttta
     5101 cctgagttcc aaaggataat gccttaaata tttgtcctcc ttctacttca ctcccaactt
     5161 agattttgcc ccacccacaa agtctttgtc ttcatttagc ttgagcaaat tctctaaata
     5221 actctgtgac atagccaccc ataccaaagg ttagtgctga gttatatacc atcgaattgc
     5281 agtccttgtt cttagaaaca cctgtggaag gaataatgaa tgctattgct gattatttat
     5341 gtttgtcttc agggactgta cctcattcta gttttgtatc cttgttccac ttttgatagt
     5401 atttacacca cagctctgta caaaacatag gcactaatta aatacataaa ttatttggcc
     5461 aggcatggtg gctcacgcct gtaatcccag cactttggga ggccaaggtg ggtgtatcac
     5521 aaggtcagga gttcaagacc agcctggcca agatggtgaa accccatctc tactaaaaat
     5581 acaaaaaatt tgccggcgtg gtggcgggcg cctgtaattc cagctacttg ggaggctgag
     5641 gcaaataatt gcttgaacca gggaggtgga ggttgcagtg aactgagatg gcaccactgc
     5701 actccagcct gggggacaga gcgagactcc acctcaaaaa aaaaaaaaga aaaaaattat
     5761 agcttcagac ttgtctaggc tcacttcagt tatattttat ctagaccagt gcttcacaaa
     5821 cgttttgatc tcaggacctc attatactaa aaaattattg aaaactggct gggcacagtg
     5881 tttcacactt gtaatcccag cactttggga ggccgaggtg gatggatcac ctgagcccag
     5941 gagtttgaga tcagcctggg caacacagta aaaccccatc tctacaaaaa aaatacaaaa
     6001 attagccagg catggtgatg cacacctata gttccagcta cttgggaggc tgaggtggta
     6061 ggatcacttg agcctgggag gtggaggttg cagtgagccg agatcatggc actgcactcc
     6121 agcctgggtg acagagtgag accctgcctc agaaaaaaaa aaaatatatc ccaatgagct
     6181 tttgtttgtg tgagctatat ttgtcaatat ttaccattgt agaaactaga caaaaatttt
     6241 aaacgcaaga atataaaagg acatattcca ctggctgtca gaacaatatc atcacacatc
     6301 ttgtagcatc tggaaaacta taccatcata agaatgacaa ggaaaaggca aataacatct
     6361 taatattact atgaaaatag ttttgacctc ataaacccct aaaaacgtct tgaagactcc
     6421 ccaggacctt ggaccacatt ttaagaactg ctgctttaca taatgtcaca tcagcgaatt
     6481 catctcttct ttttttaaaa taagaggcag ggtttcacta tgttgtccag actggtctca
     6541 aactcctggt ctcaaggaat cctcctatct tggccttcca aagtgctgga atcacaggca
     6601 tgcggcactg cgcccggcct atctcctctt tagtcagtca gtgatctgtg tattagtttt
     6661 atcgctgtgt aaaaaattgc cacacatata ttggcttaaa acaatacatt atctctgtag
     6721 gttagaaatc caggcaaaga aggttttctg ctcaagatct caaaaggctg aaatcaagat
     6781 gatggccaag gctgagttct gatctgcaac tctgaatcct tttcagatac cttcagatgg
     6841 ttgttggcag aattcagttc cttgcagttg taggatttgt ggtggcctgc ttcctatctt
     6901 ttaaggactc acatgactga gtcagtgtaa tctccctttt gattaattca aggtcaactg
     6961 attaggatca taattacgtc ccttttgcca tgttaatgta acataatcag aggaatgaca
     7021 tgtcatgata ttcacagatc ccacccatac taacggatag tagattatcc agggcatgta
     7081 aatcaggggg tggaatatta ggggacatct tttttttttt tctttaagac ggagtctcac
     7141 tctgtcaccc aggctggagt gctgtggtgc aatctcaact cactgcaacc tctacctccc
     7201 tggttcaagc gattctcgtg cctcagccgc ctgactagct gggatttcag gagtgcacca
     7261 ccagcccggc taacttttgt atttttagca gagatggggt ttcaccatgt tggccgggct
     7321 ggtctcgaac tcctgggctc aagtgatccg cccacctcgg cttcccaaag tgctggaatt
     7381 acaggcgtca gccaccatgc ccgttctggg ggccatctga gacttctgcc aaccacaatc
     7441 agctagatgt tttttgtttt gttttgaaaa aataataata ataacctttc acttgggcca
     7501 ccaaactcct accttagtat ccaagttctc taattttttt ttaatatggg aaatccacat
     7561 ctcccaaact ctatcccttt tctggcaagt tacttgacct ttctgtaaaa taagcataat
     7621 gtacctgcgt tagagttgta gggagcacta aacattcaga tgatgtatgt acagtgttta
     7681 gcacagagcc tgctaaatag taagccctca agccctcagt cacatttgtc tccatcagtg
     7741 gtagctcccc tggtgtgtta aggaggctct tctttctttt tttttttttt tttttttttt
     7801 gagacagagt ctcgctctgt tgcccaggct gaagtgcagt ggcgcgatct cggctcactg
     7861 caagctccgc ctcctgggtt cacgccattg tcctgcctca gcctcccgag tagctgggat
     7921 tacaggcgcc tgtcaccacg cccggctaat tttttttttg tttgtttttt ttgtattttt
     7981 agtagagaca gggtttcact gtgttagcca ggatggtctc catctcctca cctcgtgatc
     8041 tgcccgcctc ggcctcccaa agtgctggga ttacaggcgt gagccactgc gcccggccag
     8101 gaggctcttt ttaaactaag ggcagcaacc ccctagggtg gctatagtcc aagaaaaccc
     8161 agaaaataca tgctgccaag gatttggaga aattggagcc ctcctacatt gctggtagga
     8221 atgtagaatg gtgcagcagc tgtggaaaac agttcagcag tttcacaaaa atcaaacata
     8281 gggttatcat atgacccaga aattctaccc ataggtgtat accctggaga attaaaaaca
     8341 tgtatttaca caaaaactta tatgttcgca gcagcgttat tcataataac ccgaaagtag
     8401 aaacaaccca aatgctctac taatgaacag ataaacaaaa tgtggtatat ccatatagtg
     8461 gaatattact cgatcacaca aaggaatgaa gtactaatac atgctacagt gtggatgaac
     8521 cttgtaaaca gtaggctaag ttacagaagc cagccacaaa agacaacaaa tgcatgattc
     8581 tattcatatg aaatgtccag agtaggcaag tccatagaca gaaagtagct tagtggtttc
     8641 caggggcagt gagaagggga aatggagggc aactcctaat gggtatgagg cttcttttgg
     8701 aggtgatgaa aatgttctgg aattcgacag tggtgatggt tgtacatctc tgtgaatata
     8761 ctaaaagcca ctgaattgta tactgggtga attttatggt atctgtagac attccaattg
     8821 acagtcacat cttagtatag actttgagtc atctcactgg aggcactaga catgcaagag
     8881 gagaagtctc cagattattc cagccctgcc atttgagtca ctccaggctt tctggtcttc
     8941 ccagctgagg ctcagacacc ttggaagaga gaaaagctct cccttctgtg ccctgtgatt
     9001 tgctgatcca cagagttcat aggcatgatt aaatggtagt agttttccac cacgaagttt
     9061 tggaataatt tgtcacaaag caataggtaa ttagaaaaat aacctttcag aaggagacag
     9121 tttctccaaa gcttgggtac tttcgcttaa tattaaaaga ggaagaaatt ttttgcctgc
     9181 caggcaaaag gcagaagatg tatttctaca ccatggatag gaaaatgatt ttttttcctc
     9241 ctttctcctt ggcctgcatg cagcagtgaa aaagaaacca atggattctt ttaaatcttt
     9301 aaccatatgc agatgaataa gcacatggtt ggacttgatt cctaatctct tttcctttcc
     9361 tgtataactg agattttttt ccactctaat aaacttgagc atttaaacag aa
//
