LOCUS       NM_001098633            1775 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens AKT1 substrate 1 (AKT1S1), transcript variant 3, mRNA.
ACCESSION   NM_001098633
VERSION     NM_001098633.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 1775)
  AUTHORS   Zhang,T., Wang,Y., Yu,H., Zhang,T., Guo,L., Xu,J., Wei,X., Wang,N.,
            Wu,Y., Wang,X. and Huang,L.
  TITLE     PGK1 represses autophagy-mediated cell death to promote the
            proliferation of liver cancer cells by phosphorylating PRAS40
  JOURNAL   Cell Death Dis 13 (1), 68 (2022)
   PUBMED   35058442
  REMARK    GeneRIF: PGK1 represses autophagy-mediated cell death to promote
            the proliferation of liver cancer cells by phosphorylating PRAS40.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1775)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 1775)
  AUTHORS   Kim,K., Lee,S., Kang,H., Shin,E., Kim,H.Y., Youn,H. and Youn,B.
  TITLE     Dual Specificity Kinase DYRK3 Promotes Aggressiveness of
            Glioblastoma by Altering Mitochondrial Morphology and Function
  JOURNAL   Int J Mol Sci 22 (6), 2982 (2021)
   PUBMED   33804169
  REMARK    GeneRIF: Dual Specificity Kinase DYRK3 Promotes Aggressiveness of
            Glioblastoma by Altering Mitochondrial Morphology and Function.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1775)
  AUTHORS   Jin,J., Smith,F.D., Stark,C., Wells,C.D., Fawcett,J.P.,
            Kulkarni,S., Metalnikov,P., O'Donnell,P., Taylor,P., Taylor,L.,
            Zougman,A., Woodgett,J.R., Langeberg,L.K., Scott,J.D. and Pawson,T.
  TITLE     Proteomic, functional, and domain-based analysis of in vivo 14-3-3
            binding proteins involved in cytoskeletal regulation and cellular
            organization
  JOURNAL   Curr Biol 14 (16), 1436-1450 (2004)
   PUBMED   15324660
REFERENCE   7  (bases 1 to 1775)
  AUTHORS   Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
            Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
  TITLE     Large-scale characterization of HeLa cell nuclear phosphoproteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
   PUBMED   15302935
REFERENCE   8  (bases 1 to 1775)
  AUTHORS   Meek,S.E., Lane,W.S. and Piwnica-Worms,H.
  TITLE     Comprehensive proteomic analysis of interphase and mitotic
            14-3-3-binding proteins
  JOURNAL   J Biol Chem 279 (31), 32046-32054 (2004)
   PUBMED   15161933
REFERENCE   9  (bases 1 to 1775)
  AUTHORS   Saito,A., Narasimhan,P., Hayashi,T., Okuno,S., Ferrand-Drake,M. and
            Chan,P.H.
  TITLE     Neuroprotective role of a proline-rich Akt substrate in apoptotic
            neuronal cell death after stroke: relationships with nerve growth
            factor
  JOURNAL   J Neurosci 24 (7), 1584-1593 (2004)
   PUBMED   14973226
REFERENCE   10 (bases 1 to 1775)
  AUTHORS   Kovacina,K.S., Park,G.Y., Bae,S.S., Guzzetta,A.W., Schaefer,E.,
            Birnbaum,M.J. and Roth,R.A.
  TITLE     Identification of a proline-rich Akt substrate as a 14-3-3 binding
            partner
  JOURNAL   J Biol Chem 278 (12), 10189-10194 (2003)
   PUBMED   12524439
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA848626.1 and BC051844.2.
            
            On Jun 2, 2019 this sequence version replaced NM_001098633.3.
            
            Summary: AKT1S1 is a proline-rich substrate of AKT (MIM 164730)
            that binds 14-3-3 protein (see YWHAH, MIM 113508) when
            phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied
            by OMIM, Mar 2008].
            
            Transcript Variant: This variant (3) differs in the 5' UTR and
            coding sequence compared to variant 1. The resulting isoform (b) is
            shorter at the N-terminus compared to isoform a. Variants 2, 3, 4,
            and 5 encode the same isoform (b).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038196.2466897.1,
                                           SRR11853565.15684.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000344175.10/ ENSP00000341698.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-226               DA848626.1         35-260
            227-1775            BC051844.2         363-1911
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.33"
     gene            1..1775
                     /gene="AKT1S1"
                     /gene_synonym="Lobe; PRAS40"
                     /note="AKT1 substrate 1"
                     /db_xref="GeneID:84335"
                     /db_xref="HGNC:HGNC:28426"
                     /db_xref="MIM:610221"
     exon            1..119
                     /gene="AKT1S1"
                     /gene_synonym="Lobe; PRAS40"
                     /inference="alignment:Splign:2.1.0"
     exon            120..505
                     /gene="AKT1S1"
                     /gene_synonym="Lobe; PRAS40"
                     /inference="alignment:Splign:2.1.0"
     CDS             127..897
                     /gene="AKT1S1"
                     /gene_synonym="Lobe; PRAS40"
                     /note="isoform b is encoded by transcript variant 3; 40
                     kDa proline-rich AKT substrate; AKT1 substrate 1 (proline
                     rich)"
                     /codon_start=1
                     /product="proline-rich AKT1 substrate 1 isoform b"
                     /protein_id="NP_001092103.1"
                     /db_xref="CCDS:CCDS12784.1"
                     /db_xref="GeneID:84335"
                     /db_xref="HGNC:HGNC:28426"
                     /db_xref="MIM:610221"
                     /translation="MASGRPEELWEAVVGAAERFRARTGTELVLLTAAPPPPPRPGPC
                     AYAAHGRGALAEAARRCLHDIALAHRAATAARPPAPPPAPQPPSPTPSPPRPTLARED
                     NEEDEDEPTETETSGEQLGISDNGGLFVMDEDATLQDLPPFCESDPESTDDGSLSEET
                     PAGPPTCSVPPASALPTQQYAKSLPVSVPVWGFKEKRTEARSSDEENGPPSSPDLDRI
                     AASMRALVLREAEDTQVFGDLPRPRLNTSDFQKLKRKY"
     exon            506..583
                     /gene="AKT1S1"
                     /gene_synonym="Lobe; PRAS40"
                     /inference="alignment:Splign:2.1.0"
     exon            584..753
                     /gene="AKT1S1"
                     /gene_synonym="Lobe; PRAS40"
                     /inference="alignment:Splign:2.1.0"
     exon            754..1775
                     /gene="AKT1S1"
                     /gene_synonym="Lobe; PRAS40"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1749..1754
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AKT1S1"
                     /gene_synonym="Lobe; PRAS40"
                     /note="hexamer: ATTAAA"
     polyA_site      1775
                     /gene="AKT1S1"
                     /gene_synonym="Lobe; PRAS40"
                     /note="major polyA site"
ORIGIN      
        1 accattttgc tcgagggcat ggcctaagcc ggtcagctaa ggccatgtta atacggggct
       61 gtcccatctc tctgcggggc gcgacagctg gaagagccga acggataaga gaagaggagg
      121 gcgcggatgg cgtcggggcg ccccgaggag ctgtgggagg ccgtggtggg ggccgctgag
      181 cgcttccggg cccggactgg cacggagctg gtgctgctga ccgcggcccc gccgccacca
      241 ccccgcccgg gcccctgtgc ctatgctgcc catggtcgag gagccctggc ggaggcagcg
      301 cgccgttgcc tccacgacat cgcactggcc cacagggctg ccactgctgc tcggcctcct
      361 gcgcccccac cagcaccaca gccacccagt cccacaccca gcccaccccg gcctaccctg
      421 gccagagagg acaacgagga ggacgaggat gagcccacag agacagagac ctccggggag
      481 cagctgggca ttagtgataa tggagggctc tttgtgatgg atgaggacgc caccctccag
      541 gaccttcccc ccttctgtga gtcagacccc gagagtacag atgatggcag cctgagcgag
      601 gagacccccg ccggcccccc cacctgctca gtgcccccag cctcagccct acccacacag
      661 cagtacgcca agtccctgcc tgtgtctgtg cccgtctggg gcttcaagga gaagaggaca
      721 gaggcgcggt catcagatga ggagaatggg ccgccctctt cgcccgacct ggaccgcatc
      781 gcggcgagca tgcgcgcgct ggtgctgcga gaggccgagg acacccaggt cttcggggac
      841 ctgccacggc cgcggcttaa caccagcgac ttccagaagc tgaagcggaa atattgaagt
      901 ccagggaggg agcgccccgg gccgcgtccg ccccgtccca cactacgccc ccgccccact
      961 cccggggcct gctaatctga ggccgatccg ggaccggcct ccttgcgtct cccattccca
     1021 agattgtccc gcctctgcca atccccgccg tccttccagc ccacgacctg ccgcgccgag
     1081 gagcggcatc tgtcccgttt cccgattggg tctgtcgtct ctctccgcct agcgacagat
     1141 tccttctatt aagggattgg ctcgctgagt tctaagctct aaatgggtca actcctttgt
     1201 tttccgccta gcgacaaggg atttgctcgc acggcattgg ctccatcccc tagtcgctgg
     1261 acagctcttt ttttgattgg ctcaaatcct gtaaagggct tgaccagtct ctacatagtc
     1321 accgtccgct tttcctgagt tctccctccc aattggctcc agcttcctgg gggcgtggcc
     1381 aagccctcct cttcccagaa ttggcccggg gccttcaatt tacgttcttt acactacggg
     1441 gactggggtc gtctttgccc acgtcccgac aacttgttcc ctgaccccct cagggatggc
     1501 cccaaactgt ccctgcctct ggcaccccct ttcattggtt ccatccatcc ccacaacagc
     1561 ctgccaatcg aagcccgtcc ctgcatccag gatggtacca gctcccgccc ctcgcccccc
     1621 acctccacag gtgccttaaa gggccctcgt ccacccaagg tggggggcag gggccctcac
     1681 tctccggccc tggtgtgggg gagagagtga ggggttgggg gatcggcagt tgggaggggc
     1741 gctctgagat taaagagttt tacctctgag ataaa
//
