LOCUS       NM_001353               6543 bp    mRNA    linear   PRI 27-APR-2025
DEFINITION  Homo sapiens aldo-keto reductase family 1 member C1 (AKR1C1), mRNA.
ACCESSION   NM_001353 XM_001724839
VERSION     NM_001353.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Paul,M., Barreda,A.P., Gregson,A., Kahl,R., King,M., Hussein,W.M.,
            Walker,F.R., Smith,R., Zakar,T. and Paul,J.W.
  TITLE     Regulation of 20alpha-Hydroxysteroid Dehydrogenase Expression in
            Term Pregnant Human Myometrium Ex Vivo
  JOURNAL   Reprod Sci 31 (1), 150-161 (2024)
   PUBMED   37648943
  REMARK    GeneRIF: Regulation of 20alpha-Hydroxysteroid Dehydrogenase
            Expression in Term Pregnant Human Myometrium Ex Vivo.
REFERENCE   2  (bases 1 to 6543)
  AUTHORS   Kaftalli,J., Bonetti,G., Marceddu,G., Dhuli,K., Maltese,P.E.,
            Donato,K., Herbst,K.L., Michelini,S., Chiurazzi,P., Hill,M.,
            Michelini,S., Michelini,S., Bernini,A. and Bertelli,M.
  TITLE     AKR1C1 and hormone metabolism in lipedema pathogenesis: a
            computational biology approach
  JOURNAL   Eur Rev Med Pharmacol Sci 27 (6 Suppl), 137-147 (2023)
   PUBMED   38112954
  REMARK    GeneRIF: AKR1C1 and hormone metabolism in lipedema pathogenesis: a
            computational biology approach.
REFERENCE   3  (bases 1 to 6543)
  AUTHORS   Paul,M., Zakar,T., Phung,J., Gregson,A., Barreda,A.P., Butler,T.A.,
            Walker,F.R., Pennell,C., Smith,R. and Paul,J.W.
  TITLE     20alpha-Hydroxysteroid Dehydrogenase Expression in the Human
            Myometrium at Term and Preterm Birth: Relationships to Fetal Sex
            and Maternal Body Mass Index
  JOURNAL   Reprod Sci 30 (8), 2512-2523 (2023)
   PUBMED   36765000
  REMARK    GeneRIF: 20alpha-Hydroxysteroid Dehydrogenase Expression in the
            Human Myometrium at Term and Preterm Birth: Relationships to Fetal
            Sex and Maternal Body Mass Index.
REFERENCE   4  (bases 1 to 6543)
  AUTHORS   Zuo,X., Zeng,H., Wang,B., Yang,X., He,D., Wang,L., Ouyang,H. and
            Yuan,J.
  TITLE     AKR1C1 Protects Corneal Epithelial Cells Against Oxidative
            Stress-Mediated Ferroptosis in Dry Eye
  JOURNAL   Invest Ophthalmol Vis Sci 63 (10), 3 (2022)
   PUBMED   36066316
  REMARK    GeneRIF: AKR1C1 Protects Corneal Epithelial Cells Against Oxidative
            Stress-Mediated Ferroptosis in Dry Eye.
REFERENCE   5  (bases 1 to 6543)
  AUTHORS   Phoo,N.L.L., Dejkriengkraikul,P., Khaw-On,P. and Yodkeeree,S.
  TITLE     Transcriptomic Profiling Reveals AKR1C1 and AKR1C3 Mediate
            Cisplatin Resistance in Signet Ring Cell Gastric Carcinoma via
            Autophagic Cell Death
  JOURNAL   Int J Mol Sci 22 (22), 12512 (2021)
   PUBMED   34830394
  REMARK    GeneRIF: Transcriptomic Profiling Reveals AKR1C1 and AKR1C3 Mediate
            Cisplatin Resistance in Signet Ring Cell Gastric Carcinoma via
            Autophagic Cell Death.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6543)
  AUTHORS   Khanna,M., Qin,K.N. and Cheng,K.C.
  TITLE     Distribution of 3 alpha-hydroxysteroid dehydrogenase in rat brain
            and molecular cloning of multiple cDNAs encoding structurally
            related proteins in humans
  JOURNAL   J Steroid Biochem Mol Biol 53 (1-6), 41-46 (1995)
   PUBMED   7626489
REFERENCE   7  (bases 1 to 6543)
  AUTHORS   Khanna,M., Qin,K.N., Klisak,I., Belkin,S., Sparkes,R.S. and
            Cheng,K.C.
  TITLE     Localization of multiple human dihydrodiol dehydrogenase (DDH1 and
            DDH2) and chlordecone reductase (CHDR) genes in chromosome 10 by
            the polymerase chain reaction and fluorescence in situ
            hybridization
  JOURNAL   Genomics 25 (2), 588-590 (1995)
   PUBMED   7789999
REFERENCE   8  (bases 1 to 6543)
  AUTHORS   Ciaccio,P.J. and Tew,K.D.
  TITLE     cDNA and deduced amino acid sequences of a human colon dihydrodiol
            dehydrogenase
  JOURNAL   Biochim Biophys Acta 1186 (1-2), 129-132 (1994)
   PUBMED   8011662
REFERENCE   9  (bases 1 to 6543)
  AUTHORS   Ciaccio,P.J., Jaiswal,A.K. and Tew,K.D.
  TITLE     Regulation of human dihydrodiol dehydrogenase by Michael acceptor
            xenobiotics
  JOURNAL   J Biol Chem 269 (22), 15558-15562 (1994)
   PUBMED   7515059
REFERENCE   10 (bases 1 to 6543)
  AUTHORS   Lou,H., Hammond,L., Sharma,V., Sparkes,R.S., Lusis,A.J. and
            Stolz,A.
  TITLE     Genomic organization and chromosomal localization of a novel human
            hepatic dihydrodiol dehydrogenase with high affinity bile acid
            binding
  JOURNAL   J Biol Chem 269 (11), 8416-8422 (1994)
   PUBMED   8132567
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC040210.1, CB119749.1,
            M86609.1 and AL713867.6.
            
            On Nov 22, 2018 this sequence version replaced NM_001353.5.
            
            Summary: This gene encodes a member of the aldo/keto reductase
            superfamily, which consists of more than 40 known enzymes and
            proteins. These enzymes catalyze the conversion of aldehydes and
            ketones to their corresponding alcohols by utilizing NADH and/or
            NADPH as cofactors. The enzymes display overlapping but distinct
            substrate specificity. This enzyme catalyzes the reaction of
            progesterone to the inactive form 20-alpha-hydroxy-progesterone.
            This gene shares high sequence identity with three other gene
            members and is clustered with those three genes at chromosome
            10p15-p14. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.26350.1,
                                           SRR1660805.179145.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000380872.9/ ENSP00000370254.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-470               BC040210.1         813-1282
            471-475             CB119749.1         440-444
            476-1026            BC040210.1         1288-1838
            1027-1226           M86609.1           1003-1202
            1227-6543           AL713867.6         9633-14949
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p15.1"
     gene            1..6543
                     /gene="AKR1C1"
                     /gene_synonym="2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1;
                     DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB"
                     /note="aldo-keto reductase family 1 member C1"
                     /db_xref="GeneID:1645"
                     /db_xref="HGNC:HGNC:384"
                     /db_xref="MIM:600449"
     exon            1..114
                     /gene="AKR1C1"
                     /gene_synonym="2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1;
                     DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB"
                     /inference="alignment:Splign:2.1.0"
     CDS             31..1002
                     /gene="AKR1C1"
                     /gene_synonym="2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1;
                     DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB"
                     /EC_number="1.1.1.62"
                     /EC_number="1.3.1.20"
                     /EC_number="1.1.1.149"
                     /EC_number="1.1.1.112"
                     /EC_number="1.1.1.209"
                     /EC_number="1.1.1.51"
                     /EC_number="1.1.1.357"
                     /EC_number="1.1.1.210"
                     /EC_number="1.1.1.53"
                     /note="dihydrodiol dehydrogenase 1; 20-alpha
                     (3-alpha)-hydroxysteroid dehydrogenase; aldo-keto
                     reductase C; trans-1,2-dihydrobenzene-1,2-diol
                     dehydrogenase; 20 alpha-hydroxysteroid dehydrogenase; type
                     II 3-alpha-hydroxysteroid dehydrogenase; hepatic
                     dihydrodiol dehydrogenase; indanol dehydrogenase;
                     dihydrodiol dehydrogenase 1/2; chlordecone reductase
                     homolog HAKRC; high-affinity hepatic bile acid-binding
                     protein"
                     /codon_start=1
                     /product="aldo-keto reductase family 1 member C1"
                     /protein_id="NP_001344.2"
                     /db_xref="CCDS:CCDS7061.1"
                     /db_xref="GeneID:1645"
                     /db_xref="HGNC:HGNC:384"
                     /db_xref="MIM:600449"
                     /translation="MDSKYQCVKLNDGHFMPVLGFGTYAPAEVPKSKALEATKLAIEA
                     GFRHIDSAHLYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWCNSHRPELVRPALERS
                     LKNLQLDYVDLYLIHFPVSVKPGEEVIPKDENGKILFDTVDLCATWEAVEKCKDAGLA
                     KSIGVSNFNRRQLEMILNKPGLKYKPVCNQVECHPYFNQRKLLDFCKSKDIVLVAYSA
                     LGSHREEPWVDPNSPVLLEDPVLCALAKKHKRTPALIALRYQLQRGVVVLAKSYNEQR
                     IRQNVQVFEFQLTSEEMKAIDGLNRNVRYLTLDIFAGPPNYPFSDEY"
     exon            115..282
                     /gene="AKR1C1"
                     /gene_synonym="2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1;
                     DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB"
                     /inference="alignment:Splign:2.1.0"
     exon            283..399
                     /gene="AKR1C1"
                     /gene_synonym="2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1;
                     DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB"
                     /inference="alignment:Splign:2.1.0"
     exon            400..477
                     /gene="AKR1C1"
                     /gene_synonym="2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1;
                     DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB"
                     /inference="alignment:Splign:2.1.0"
     exon            478..600
                     /gene="AKR1C1"
                     /gene_synonym="2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1;
                     DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB"
                     /inference="alignment:Splign:2.1.0"
     exon            601..710
                     /gene="AKR1C1"
                     /gene_synonym="2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1;
                     DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB"
                     /inference="alignment:Splign:2.1.0"
     exon            711..876
                     /gene="AKR1C1"
                     /gene_synonym="2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1;
                     DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB"
                     /inference="alignment:Splign:2.1.0"
     exon            877..959
                     /gene="AKR1C1"
                     /gene_synonym="2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1;
                     DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB"
                     /inference="alignment:Splign:2.1.0"
     exon            960..6543
                     /gene="AKR1C1"
                     /gene_synonym="2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1;
                     DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1194..1199
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AKR1C1"
                     /gene_synonym="2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1;
                     DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB"
                     /note="hexamer: ATTAAA"
     polyA_site      1226
                     /gene="AKR1C1"
                     /gene_synonym="2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1;
                     DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB"
                     /note="major polyA site"
     regulatory      3414..3419
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AKR1C1"
                     /gene_synonym="2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1;
                     DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB"
                     /note="hexamer: AGTAAA"
     polyA_site      3433
                     /gene="AKR1C1"
                     /gene_synonym="2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1;
                     DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB"
     regulatory      4422..4427
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AKR1C1"
                     /gene_synonym="2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1;
                     DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB"
                     /note="hexamer: AGTAAA"
     polyA_site      4454
                     /gene="AKR1C1"
                     /gene_synonym="2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1;
                     DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB"
     regulatory      6523..6528
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AKR1C1"
                     /gene_synonym="2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1;
                     DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB"
                     /note="hexamer: AATAAA"
     polyA_site      6543
                     /gene="AKR1C1"
                     /gene_synonym="2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1;
                     DD1/DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB"
ORIGIN      
        1 aaacatttgc cagccaggct agtgacagaa atggattcga aatatcagtg tgtgaagctg
       61 aatgatggtc acttcatgcc tgtcctggga tttggcacct atgcgcctgc agaggttcct
      121 aaaagtaaag ctttagaggc caccaaattg gcaattgaag ctggcttccg ccatattgat
      181 tctgctcatt tatacaataa tgaggagcag gttggactgg ccatccgaag caagattgca
      241 gatggcagtg tgaagagaga agacatattc tacacttcaa agctttggtg caattcccat
      301 cgaccagagt tggtccgacc agccttggaa aggtcactga aaaatcttca attggattat
      361 gttgacctct accttattca ttttccagtg tctgtaaagc caggtgagga agtgatccca
      421 aaagatgaaa atggaaaaat actatttgac acagtggatc tctgtgccac atgggaggcc
      481 gtggagaagt gtaaagatgc aggattggcc aagtccatcg gggtgtccaa cttcaaccgc
      541 aggcagctgg agatgatcct caacaagcca gggctcaagt acaagcctgt ctgcaaccag
      601 gtggaatgtc atccttactt caaccagaga aaactgctgg atttctgcaa gtcaaaagac
      661 attgttctgg ttgcctatag tgctctggga tcccaccgag aagaaccatg ggtggacccg
      721 aactccccgg tgctcttgga ggacccagtc ctttgtgcct tggcaaaaaa gcacaagcga
      781 accccagccc tgattgccct gcgctaccag ctacagcgtg gggttgtggt cctggccaag
      841 agctacaatg agcagcgcat cagacagaac gtgcaggtgt ttgaattcca gttgacttca
      901 gaggagatga aagccataga tggcctaaac agaaatgtgc gatatttgac ccttgatatt
      961 tttgctggcc cccctaatta tccattttct gatgaatatt aacatggagg gcattgcatg
     1021 aggtctgcca gaaggccctg cgtgtggatg gtgacacaga ggatggctct atgctggtga
     1081 ctggacacat cgcctctggt taaatctctc ctgcttggtg atttcagcaa gctacagcaa
     1141 agcccattgg ccagaaagga aagacaataa ttttgttttt tcattttgaa aaaattaaat
     1201 gctctctcct aaagattctt cacctacttt ggtctccata acttctatgt tttctttcct
     1261 tctgacacac tagtgcccct aaattgtgat ttgcctatac gtttagggcc ggggttggaa
     1321 gatgttaaca accatttaag attcatttct gcagtgggag tgggtggagt ttcaccctct
     1381 gggaaagggg caggtgacag gtatttatca gtcagtgcct ctctagctct tgtaggaaga
     1441 agcacacgca ggatggagtc tagaggatga gcgatattga ctagcaattc atgggctccc
     1501 tccagcagtg cgagggtcag agtttctgga gccttgggag gaggcatccc tgtgaggggg
     1561 ggttagggag atgggagggc accaggaaaa gtgattagaa gtcaggtatg ggaaggctaa
     1621 ataggacaga gtcgagtaca tctctgcttg gaaaaacata tcaacaccct tttttttgaa
     1681 cattatatct tgctcataaa agaaaacttt ccacattgtt ttaacaaacc ccacagctga
     1741 gagtcaggcc tgaatctttg atgtgtgccc attcacaacg ttgaccctat tggtttgtgg
     1801 tggggcaggg catcaaagac atcattgact aatcacattc ccctgaatag ctcatattta
     1861 gaaaatattc ttagattcta aaaatgtact attaatttgt gatattcagt cttttaaata
     1921 ttttatacat taaacaggca tagttacaaa tataaaacaa aaatatccca aagccattat
     1981 gcatggcact caagattaaa atgggaaata atacatctaa taaatcaaat gttccaagac
     2041 ttcaaaggtc ttttggaaac aggctatgta aaacagcaca ctggtttcaa actttggtaa
     2101 attttaagaa caactcttac aaaggcattt aattcttata cataattttc aggggaccta
     2161 agttaatcag ctaatcatga agacatgatt ttcattttag aaaacacttt tgaaaacttg
     2221 ggataatctc atgccttaat gatcaaagca ttatgagaag gacagtggtt tttaacctgg
     2281 gcatatgttc taacacattt actctccact attcgtactc tggtagccat gttaacccca
     2341 tcagagattc cttctcaagc catgtctcag agctgagagg catcccagca agttttgcag
     2401 ctcacagttt tttccgtaaa ttacttattc tataaaattg gagtaggcca taaactttgg
     2461 agggccctag accaattttt tggattattt ttcgtcttct atcattccgc tgatcttaga
     2521 tattctctgc attaaatatt aaatatcact tctaggctga aaaatccccc taaaaatatt
     2581 tctagctcag atttttcctc caaattctgc aatagaagat cacaatgtga actctgcatc
     2641 tccatgttaa agtctaatgg acattcacac ttagcatgtc tcaaagaaat ctcatgtaaa
     2701 ccatggccat cctgttctac cttaactttc tgagtctatg gaatgataat ttcacatctc
     2761 ataaacttga ctgatgtaag tgtcaagaaa agattgacat tttgttaaaa gttagtagtg
     2821 aagtgtgtaa cgcttaagca aactttcata tttcaaatct ctttagcaag tgtaactctt
     2881 ttttcaagat gtgaaataat cattaggtca gtcatttgta aatagtacat ctgctatgga
     2941 ctttttccag ttcttcacca tccattttta taaaactctt attgttaaaa aaaaagttac
     3001 tcagaatttc ataaagccaa acacctgatt tcaggaacac ttgagatgta agaaaatttt
     3061 atagggacct ccaatcacta attttcctat tttttctctc aaagaaatgc tgaagggagg
     3121 aattcaggtt gaatgaaagg aaatagtaac ttacagccat atagagttat aaagacttct
     3181 tgtaaatgtg aacatatggt aaaatataaa aacatgtatt tttgaaattt tggattctac
     3241 tcattatttt acttcaattt aagatataaa tgtatagaaa taagtataat tctaagctaa
     3301 tacgtatgca atgtaggaag ctgtaattac tgaccaaaac tatgtgaagt ggagaaaacc
     3361 tggggaagtg gatggtttta gatgaaactg aagttaaatt catattgatt taaagtaaat
     3421 tgttataact ttataaagtt tttcatcatc accacagcaa tcacaaagag aataattatg
     3481 aatatacgca agaggaaatg agaagggaat ccaaatgtca ttaaaaaaaa atcacgccac
     3541 ctcacaaaat ggtaacagtg gatataaagg atagaaagct ataaacctgg taggaaaaca
     3601 ttgaaaatgg cacaagtgac tccttccctg tcaccaatga cagtcatgtg ttgcataatg
     3661 cctgggctat cttctgagaa tggctttgtt aggcaactgt gtcatggtgg ggacatgata
     3721 gcattagaga gaattctctc aaacactgtc atggccttat caactatgct tctgtaatat
     3781 tctgaatcct tcgctgcctt tccaacagtg tttacaacgg tttcctaaga aatgaaatcc
     3841 atttcaccaa accacttttc ttgctcatcc ataagaaaca cttctccttt gttaaaatta
     3901 catcatgaga ttgcaagcat tcagtaactt gcaagctcca atttgaattc tagttgtctt
     3961 gctatttcca ccacatctgc aattactttc tccactgaaa tcttgcattc ctccaagtca
     4021 tccaggaggg ttgaattcac ttctttcaaa ctcctgttca agtaaatgtt ttgccctcct
     4081 gtcacgaatc atgaatgttt ttaatggcat ctagaatagt aaatcttttt cagaagattt
     4141 tcaattttct tttcccaggt tcttcagaca agtcaccatg gtagctgtag acttatgaaa
     4201 tgtatttcct gaaccataaa acaagaacat caaaattact ctatgatcca tgggaaacag
     4261 aatgcatgtt gtgttagcag gcatggaaag aacagtaatc tctttcaacc tctgtaagag
     4321 agttcttggt tgattaggta ccttgtcgat aggcagttct aatttcaaag aactttttct
     4381 tttgctgagc agtaagtctt aacagtgggc ttaaaatatt cagtaaacca ttatttaagg
     4441 agatgtggct taaatatcta ggctttcttg ctttatttat agaacactta cagagtagat
     4501 atagattaaa tcttaaggac cctaggattg ttagaatagt aaatgaacat catatgttgt
     4561 aatatagtta gccagggagg ggaaagatct ctacaagaaa taattttaaa aactactcaa
     4621 agaaatcaga gatatcacaa aatgacaaaa caagtacagg ggcagcaaaa ggtaacagat
     4681 gtcaactatg agatctcatc aagaactaag aaaatggaga aaagtaccag atggcagatg
     4741 ggaggcagtg tgagcatgcc tctcccactt ggagattagt gtgtagagat ttacattgtg
     4801 aatttctttt caagaaccaa tgcagagact taacagatat atgaaaaaat ttcacataca
     4861 ctctgaagaa gtctcaggca caatcctact ccatgaaata agaaaaaacc atcacatccc
     4921 cagattctga ggggagagag ttcactccag agcatacatc cccactgggg atctggaaat
     4981 tcaggccaca agagaaggct ttgatcctgt gcggacaagt gccttgagca gaatccacag
     5041 gggagagctg gaagtgcatg gcagatatga gcacagaagc tgggtgtcca gccttgtggg
     5101 cagaccggga gggatgtggc ctgaaagcca tggttattat ctcagcgtgt aagctgatga
     5161 tctgaggctg gtcagagttc tgtgcgcaga ctgcctggat gtaaacctgg cactgttagc
     5221 agagcactgc atgaatgaaa ggaatgaaaa tagccttgcc aactgcatag gtgctgggtg
     5281 aggctcactg tggggggcgc acggcaagct attcaacatt acgtacaggc atttgaggtc
     5341 ggggcatgga aaaatactga ggcagtgtgt gtatgttatt tgtgctgaga atgaaactcc
     5401 ttgaccttga aaacaggaca gggagtggtg tgtgtgctgt gataaggaat gctgaaaacg
     5461 gtctcctgag aatgcggttt gagtgctttt acaaggccac aggtgtctca cgacctgacc
     5521 tcataaagcc atctagtgga tgtttgtggc ttaacaagcc ccttcaataa gtacttggcg
     5581 gagggatgct ggggggacct tcttagaaga gctgcgcccc acccccctcc ctggacagcc
     5641 cagctacaat tgtctgagaa ctcactgcaa gctgtaagcg gcccactact tcccactccc
     5701 ttttttgaac tcttctgtgc agcagaggca attaaactct cctctggaac attaccccag
     5761 cagcctgaga accacccctt ggcgctcaca ggggctgcag cctgccttat ccaaggacag
     5821 ttagagtgca gacccaccta accctggacc cactggtggt acccatccac ccatcctggt
     5881 agcttaacac aaagatacac ttttaggagc ttcatagccc ctcttccccc attgcctgag
     5941 aaaccacttt ccctgggcaa cttagggcaa gctcaaatcc cactgctacc accacagctg
     6001 gtgctctttt gcaagtgcca cctcctagct ggagaccaac cagcacagtc cacacagcac
     6061 ctcaaggaag aataaataga actgttccca ggaaggagaa aatgcctgca tgagctcagc
     6121 tgttaccact gcgtaccaca ccctgaccag tcagaggtct tgagtcggtc cgcatgacaa
     6181 gttcaccgct cgcataacca gcattcaaga aagccagcac actaagccta tctacagcca
     6241 gggagtctca gagtctacgt cactcccctg ccacctcaat cagagctggt gctggtatcc
     6301 actgctggga gacttgtgct gaggtgaagc ctgggctgga tggctggagg atgaagaact
     6361 gcacggagga gatggaggca cccagacaga tgccccatgg acatcctgaa gcagagctgc
     6421 ctcactgccc tgcacacaca tgagagcgca gccaggaaca gaaggactgg gcaacattca
     6481 ggcggagccc aggccaattt tctaaacatg gaatatgagt taaataaatg gcttcgtatt
     6541 aaa
//
