LOCUS       NM_005858               3665 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens A-kinase anchoring protein 8 (AKAP8), mRNA.
ACCESSION   NM_005858
VERSION     NM_005858.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Kong,X., An,P., Xu,J., Liu,W., Lin,F. and Yang,Y.
  TITLE     A-Kinase Anchor Protein 95 Is Involved in ERK1/2-Elk-1 Signal
            Transduction in Colon Cancer
  JOURNAL   Anal Cell Pathol (Amst) 2023, 8242646 (2023)
   PUBMED   36691407
  REMARK    GeneRIF: A-Kinase Anchor Protein 95 Is Involved in ERK1/2-Elk-1
            Signal Transduction in Colon Cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 3665)
  AUTHORS   Li,W., Hu,J., Shi,B., Palomba,F., Digman,M.A., Gratton,E. and
            Jiang,H.
  TITLE     Biophysical properties of AKAP95 protein condensates regulate
            splicing and tumorigenesis
  JOURNAL   Nat Cell Biol 22 (8), 960-972 (2020)
   PUBMED   32719551
  REMARK    GeneRIF: Biophysical properties of AKAP95 protein condensates
            regulate splicing and tumorigenesis.
REFERENCE   4  (bases 1 to 3665)
  AUTHORS   Zuo,H., Trombetta-Lima,M., Heijink,I.H., van der Veen,C.H.T.J.,
            Hesse,L., Faber,K.N., Poppinga,W.J., Maarsingh,H., Nikolaev,V.O.
            and Schmidt,A.M.
  TITLE     A-Kinase Anchoring Proteins Diminish TGF-beta1/Cigarette
            Smoke-Induced Epithelial-To-Mesenchymal Transition
  JOURNAL   Cells 9 (2), 356 (2020)
   PUBMED   32028718
  REMARK    GeneRIF: A-Kinase Anchoring Proteins Diminish TGF-beta1/Cigarette
            Smoke-Induced Epithelial-To-Mesenchymal Transition.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3665)
  AUTHORS   Hu,X., Harvey,S.E., Zheng,R., Lyu,J., Grzeskowiak,C.L., Powell,E.,
            Piwnica-Worms,H., Scott,K.L. and Cheng,C.
  TITLE     The RNA-binding protein AKAP8 suppresses tumor metastasis by
            antagonizing EMT-associated alternative splicing
  JOURNAL   Nat Commun 11 (1), 486 (2020)
   PUBMED   31980632
  REMARK    GeneRIF: Identification of A-Kinase Anchor Protein (AKAP8) as a
            splicing regulatory factor that impedes EMT and breast cancer
            metastasis. AKAP8 not only is capable of inhibiting splicing
            activity of the EMT-promoting splicing regulator hnRNPM through
            protein-protein interaction, it also directly binds to RNA and
            alters splicing outcomes. Genome-wide analysis shows that AKAP8
            promotes an epithelial cell state splicing program.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3665)
  AUTHORS   Akileswaran,L., Taraska,J.W., Sayer,J.A., Gettemy,J.M. and
            Coghlan,V.M.
  TITLE     A-kinase-anchoring protein AKAP95 is targeted to the nuclear matrix
            and associates with p68 RNA helicase
  JOURNAL   J Biol Chem 276 (20), 17448-17454 (2001)
   PUBMED   11279182
REFERENCE   7  (bases 1 to 3665)
  AUTHORS   Steen,R.L., Cubizolles,F., Le Guellec,K. and Collas,P.
  TITLE     A kinase-anchoring protein (AKAP)95 recruits human
            chromosome-associated protein (hCAP)-D2/Eg7 for chromosome
            condensation in mitotic extract
  JOURNAL   J Cell Biol 149 (3), 531-536 (2000)
   PUBMED   10791967
REFERENCE   8  (bases 1 to 3665)
  AUTHORS   Collas,P., Le Guellec,K. and Tasken,K.
  TITLE     The A-kinase-anchoring protein AKAP95 is a multivalent protein with
            a key role in chromatin condensation at mitosis
  JOURNAL   J Cell Biol 147 (6), 1167-1180 (1999)
   PUBMED   10601332
REFERENCE   9  (bases 1 to 3665)
  AUTHORS   Eide,T., Coghlan,V., Orstavik,S., Holsve,C., Solberg,R.,
            Skalhegg,B.S., Lamb,N.J., Langeberg,L., Fernandez,A., Scott,J.D.,
            Jahnsen,T. and Tasken,K.
  TITLE     Molecular cloning, chromosomal localization, and cell
            cycle-dependent subcellular distribution of the A-kinase anchoring
            protein, AKAP95
  JOURNAL   Exp Cell Res 238 (2), 305-316 (1998)
   PUBMED   9473338
REFERENCE   10 (bases 1 to 3665)
  AUTHORS   Lester,L.B. and Scott,J.D.
  TITLE     Anchoring and scaffold proteins for kinases and phosphatases
  JOURNAL   Recent Prog Horm Res 52, 409-29 (1997)
   PUBMED   9238861
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from Y11997.1, CA429585.1,
            AI581319.1 and AC093060.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_005858.3.
            
            Summary: This gene encodes a member of the A-kinase anchor protein
            family. A-kinase anchor proteins are scaffold proteins that contain
            a binding domain for the RI/RII subunit of protein kinase A (PKA)
            and recruit PKA and other signaling molecules to specific
            subcellular locations. This gene encodes a nuclear A-kinase anchor
            protein that binds to the RII alpha subunit of PKA and may play a
            role in chromosome condensation during mitosis by targeting PKA and
            the condensin complex to chromatin. A pseudogene of this gene is
            located on the short arm of chromosome 9. [provided by RefSeq, May
            2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.161666.1,
                                           SRR1803614.116134.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000269701.7/ ENSP00000269701.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2940              Y11997.1           6-2945
            2941-3419           CA429585.1         120-598             c
            3420-3529           AI581319.1         7-116               c
            3530-3665           AC093060.2         3854-3989           c
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.12"
     gene            1..3665
                     /gene="AKAP8"
                     /gene_synonym="AKAP 95; AKAP-8; AKAP-95; AKAP95"
                     /note="A-kinase anchoring protein 8"
                     /db_xref="GeneID:10270"
                     /db_xref="HGNC:HGNC:378"
                     /db_xref="MIM:604692"
     exon            1..75
                     /gene="AKAP8"
                     /gene_synonym="AKAP 95; AKAP-8; AKAP-95; AKAP95"
                     /inference="alignment:Splign:2.1.0"
     CDS             57..2135
                     /gene="AKAP8"
                     /gene_synonym="AKAP 95; AKAP-8; AKAP-95; AKAP95"
                     /note="A-kinase anchor protein, 95kDa; A kinase (PRKA)
                     anchor protein 8"
                     /codon_start=1
                     /product="A-kinase anchor protein 8"
                     /protein_id="NP_005849.1"
                     /db_xref="CCDS:CCDS12329.1"
                     /db_xref="GeneID:10270"
                     /db_xref="HGNC:HGNC:378"
                     /db_xref="MIM:604692"
                     /translation="MDQGYGGYGAWSAGPANTQGAYGTGVASWQGYENYNYYGAQNTS
                     VTTGATYSYGPASWEAAKANDGGLAAGAPAMHMASYGPEPCTDNSDSLIAKINQRLDM
                     MSKEGGRGGSGGGGEGIQDRESSFRFQPFESYDSRPCLPEHNPYRPSYSYDYEFDLGS
                     DRNGSFGGQYSECRDPARERGSLDGFMRGRGQGRFQDRSNPGTFMRSDPFVPPAASSE
                     PLSTPWNELNYVGGRGLGGPSPSRPPPSLFSQSMAPDYGVMGMQGAGGYDSTMPYGCG
                     RSQPRMRDRDRPKRRGFDRFGPDGTGRKRKQFQLYEEPDTKLARVDSEGDFSENDDAA
                     GDFRSGDEEFKGEDELCDSGRQRGEKEDEDEDVKKRREKQRRRDRTRDRAADRIQFAC
                     SVCKFRSFDDEEIQKHLQSKFHKETLRFISTKLPDKTVEFLQEYIVNRNKKIEKRRQE
                     LMEKETAKPKPDPFKGIGQEHFFKKIEAAHCLACDMLIPAQPQLLQRHLHSVDHNHNR
                     RLAAEQFKKTSLHVAKSVLNNRHIVKMLEKYLKGEDPFTSETVDPEMEGDDNLGGEDK
                     KETPEEVAADVLAEVITAAVRAVDGEGAPAPESSGEPAEDEGPTDTAEAGSDPQAEQL
                     LEEQVPCGTAHEKGVPKARSEAAEAGNGAETMAAEAESAQTRVAPAPAAADAEVEQTD
                     AESKDAVPTE"
     exon            76..114
                     /gene="AKAP8"
                     /gene_synonym="AKAP 95; AKAP-8; AKAP-95; AKAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            115..147
                     /gene="AKAP8"
                     /gene_synonym="AKAP 95; AKAP-8; AKAP-95; AKAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            148..427
                     /gene="AKAP8"
                     /gene_synonym="AKAP 95; AKAP-8; AKAP-95; AKAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            428..917
                     /gene="AKAP8"
                     /gene_synonym="AKAP 95; AKAP-8; AKAP-95; AKAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            918..1047
                     /gene="AKAP8"
                     /gene_synonym="AKAP 95; AKAP-8; AKAP-95; AKAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            1048..1094
                     /gene="AKAP8"
                     /gene_synonym="AKAP 95; AKAP-8; AKAP-95; AKAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1128
                     /gene="AKAP8"
                     /gene_synonym="AKAP 95; AKAP-8; AKAP-95; AKAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            1129..1216
                     /gene="AKAP8"
                     /gene_synonym="AKAP 95; AKAP-8; AKAP-95; AKAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            1217..1358
                     /gene="AKAP8"
                     /gene_synonym="AKAP 95; AKAP-8; AKAP-95; AKAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            1359..1452
                     /gene="AKAP8"
                     /gene_synonym="AKAP 95; AKAP-8; AKAP-95; AKAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            1453..1583
                     /gene="AKAP8"
                     /gene_synonym="AKAP 95; AKAP-8; AKAP-95; AKAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            1584..1679
                     /gene="AKAP8"
                     /gene_synonym="AKAP 95; AKAP-8; AKAP-95; AKAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            1680..3665
                     /gene="AKAP8"
                     /gene_synonym="AKAP 95; AKAP-8; AKAP-95; AKAP95"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2398..2403
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AKAP8"
                     /gene_synonym="AKAP 95; AKAP-8; AKAP-95; AKAP95"
                     /note="hexamer: AATAAA"
     polyA_site      2418
                     /gene="AKAP8"
                     /gene_synonym="AKAP 95; AKAP-8; AKAP-95; AKAP95"
                     /note="major polyA site"
     regulatory      3508..3513
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AKAP8"
                     /gene_synonym="AKAP 95; AKAP-8; AKAP-95; AKAP95"
                     /note="hexamer: AATAAA"
     polyA_site      3527
                     /gene="AKAP8"
                     /gene_synonym="AKAP 95; AKAP-8; AKAP-95; AKAP95"
                     /note="major polyA site"
     regulatory      3644..3649
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AKAP8"
                     /gene_synonym="AKAP 95; AKAP-8; AKAP-95; AKAP95"
                     /note="hexamer: ATTAAA"
     polyA_site      3665
                     /gene="AKAP8"
                     /gene_synonym="AKAP 95; AKAP-8; AKAP-95; AKAP95"
ORIGIN      
        1 gcatgcgtgc tgtggtcgcc tagtaaacgg ggctgctggt gggccgcgtc gaagacatgg
       61 accagggcta cggaggctac ggggcgtgga gtgctggacc tgccaacacc cagggtgcat
      121 atggaactgg tgtggccagc tggcaaggtt atgaaaacta caattactat ggcgcccaga
      181 acaccagtgt caccacaggc gcaacctaca gctacggccc agcctcgtgg gaggccgcca
      241 aggccaatga tggcggcctg gcggccgggg cccctgccat gcacatggcc tcttacggcc
      301 cagagccatg caccgacaat tccgactccc tcattgccaa gatcaaccag cgtttggaca
      361 tgatgtccaa ggaaggaggc aggggcggga gcggcggcgg tggggagggc atacaggacc
      421 gggagagctc cttccgcttc cagccgttcg agtcctatga ctccaggccc tgcctgccgg
      481 agcacaaccc ctaccgcccc agctacagct acgactatga gttcgacctg gggtccgacc
      541 gcaatggcag ctttgggggg cagtacagtg aatgccgaga cccagcccgg gagcggggct
      601 cccttgatgg cttcatgcgg ggccggggcc agggccgctt ccaggaccgg agcaaccctg
      661 gcaccttcat gcgcagcgac cccttcgtgc cccccgctgc gtcctctgag cccctgtcca
      721 cgccctggaa cgagctgaac tacgtgggtg gacggggcct gggagggccc tcccccagcc
      781 ggccacctcc gtccctcttc tcccagtcca tggctcccga ctacggcgtg atgggcatgc
      841 agggggcggg cggctatgac agcaccatgc cctacggatg tggccgctcg cagcctcgga
      901 tgcgggatcg ggatcggccc aagaggagag ggtttgaccg cttcggacca gatggcacgg
      961 gcaggaaacg gaagcagttc caactttacg aggagccaga caccaaactg gcccgggttg
     1021 acagtgaagg agatttctcc gaaaatgatg acgcagctgg tgacttccgc tcaggagatg
     1081 aagaattcaa gggtgaggat gaactctgcg actctgggag gcaaagagga gagaaggagg
     1141 acgaggacga ggatgtgaag aagagaaggg aaaagcaaag gagaagagac aggacgcggg
     1201 accgtgcagc cgacagaatt cagtttgcct gttctgtatg caagttccgt agctttgatg
     1261 acgaagagat ccagaagcat ctgcaaagca aatttcacaa agagaccctg cggttcataa
     1321 gcaccaagct gcccgacaag accgtggagt tcctccagga atacattgta aacagaaata
     1381 agaaaattga gaagcggcgt caggaattga tggagaaaga aaccgcaaaa ccaaaaccag
     1441 atcctttcaa agggattggc caggagcact tcttcaagaa gatcgaggct gctcactgcc
     1501 tggcctgcga catgctaatt cctgcacagc cgcagctcct ccagcggcac ctgcactccg
     1561 tggaccacaa tcacaaccgc aggttggctg ctgaacagtt caagaaaacc agtctccatg
     1621 tggctaagag tgttttgaac aacagacata tagtgaagat gctggaaaaa tacctcaagg
     1681 gtgaggaccc tttcaccagt gaaactgttg atccagaaat ggaaggagat gacaatttag
     1741 gaggtgagga taagaaagag acacctgagg aggtggccgc ggacgtctta gcagaggtga
     1801 ttacagcagc agtgagggcc gtagatgggg aaggagcgcc cgctccagag agcagcgggg
     1861 agccggctga ggacgaaggc cccacggaca cagcggaggc cggtagtgat cctcaagccg
     1921 aacagctgct ggaagagcag gtgccctgtg gaacggcaca tgagaagggc gtccccaagg
     1981 ccagaagtga ggctgcagag gctggaaatg gcgccgagac aatggcagca gaggcagaaa
     2041 gtgcccaaac cagagttgct cctgccccag ctgccgcgga tgctgaagtg gaacaaactg
     2101 atgcagagtc taaagacgct gttcccacag aatgatgctc atttccctgt tccagggaag
     2161 gcgttgggat gatggatgcg ttggtctttc tcccttggtt tgtaagcagt acaagggcgt
     2221 gtgctcccag aatatgctgt aatctaattt tggtgaagag acccagcgtt tcctcctgag
     2281 cagtgcctct cacggcttgt ctcatgcagt cgtgtggctt cttgcccagg tttcaaagct
     2341 gaagtacatt gtccttagcg gctgtaacat gtctcttgac agtagtgcac ttggaataat
     2401 aaaggttggg tgattatatc ttgatgatac attacttgtt caatacagcc actgatggaa
     2461 tgcttccttt tttatttttt tccttaattt ttttttttat ttggttggga acagctgaat
     2521 actaggaata tatcttgctc tatagaggat ttttttttgt atgtttcaag cttcagcctt
     2581 taacctatac ctttgtagtg caccatatgg tgtgtgactt tcacaggact tcgcagcacc
     2641 tggttcacat gtggcactga ccgcgtcaca tccacgcact cccaaaggcc agaagtatct
     2701 gaccgaccta cgccactgga aacacaccca ccgcaacctc aagaaccaga ctgtgcagag
     2761 ggcattgcgt cccaatcttt agtccttgct gaatcagttc tctaatattt tacctcattt
     2821 gtgttccacc tctagattac ttcaggtttt tttcctttaa aattagttac taccactcaa
     2881 atgtatttac aaagagaatt tggccaggca cggtgatgca tacctataat cccagcactt
     2941 cgggaggccg tggtgagagg atagcttaag cccaggagtt caagaccaac ctggacaaca
     3001 tagcaagacc ccatctctta aaaaaaaagg aaagaaaact tgatgtgatt gccataggtg
     3061 gaataatcca acataaattg ccatagatag aaggtatctg taatatatat atatatatat
     3121 aaaatgaaat atatgtttca ttttagagaa ataactatta ctttagatct ttccaaatct
     3181 gagaaaggga ggctagcatg tgttcaaggt tagcacgcaa cagaatttcc taaaatcaga
     3241 agaattggaa gatcctcccc ttttgaaatg gccctgctgt gtcagtttcc ctgtggcctt
     3301 ttgaactgta catctcacat gttgggaaac gctggccact gggaaatcat tagaaaggag
     3361 gctgtagaat atttgccgag cctctactgt ataccagggg ctaactcacc aagcacattc
     3421 taggaattgg gccctgctca tgaggagcct tagtggagat tccaggtgaa tatttatgaa
     3481 aaagtcaaca ttagaactga aaatggaaat aaactgcttg aaaagacgat ggtgcctctg
     3541 ggtcatttct gctcagcatc cgtttgtgtt gtgtagatgc gtcttaatcg cagggtgagc
     3601 atgcctttta agcttgttct cttaaggcaa aaatgtcggt agcattaaac agtagtgcag
     3661 aaaaa
//
