LOCUS       NM_016377               3150 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens A-kinase anchoring protein 7 (AKAP7), transcript
            variant gamma, mRNA.
ACCESSION   NM_016377
VERSION     NM_016377.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   O'Connell,G.C., Treadway,M.B., Petrone,A.B., Tennant,C.S.,
            Lucke-Wold,N., Chantler,P.D. and Barr,T.L.
  TITLE     Peripheral blood AKAP7 expression as an early marker for
            lymphocyte-mediated post-stroke blood brain barrier disruption
  JOURNAL   Sci Rep 7 (1), 1172 (2017)
   PUBMED   28446746
  REMARK    GeneRIF: AKAP7 expression levels may have clinical utility as a
            prognostic biomarker for post-stroke blood brain complications
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3150)
  AUTHORS   Bjerregaard-Andersen,K., Ostensen,E., Scott,J.D., Tasken,K. and
            Morth,J.P.
  TITLE     Malonate in the nucleotide-binding site traps human
            AKAP18gamma/delta in a novel conformational state
  JOURNAL   Acta Crystallogr F Struct Biol Commun 72 (Pt 8), 591-597 (2016)
   PUBMED   27487922
  REMARK    GeneRIF: Malonate in the nucleotide-binding site traps human
            AKAP18-gamma in a novel conformational state.
REFERENCE   4  (bases 1 to 3150)
  AUTHORS   Burgers,P.P., van der Heyden,M.A., Kok,B., Heck,A.J. and
            Scholten,A.
  TITLE     A systematic evaluation of protein kinase A-A-kinase anchoring
            protein interaction motifs
  JOURNAL   Biochemistry 54 (1), 11-21 (2015)
   PUBMED   25097019
REFERENCE   5  (bases 1 to 3150)
  AUTHORS   Greenwald,E.C., Redden,J.M., Dodge-Kafka,K.L. and Saucerman,J.J.
  TITLE     Scaffold state switching amplifies, accelerates, and insulates
            protein kinase C signaling
  JOURNAL   J Biol Chem 289 (4), 2353-2360 (2014)
   PUBMED   24302730
  REMARK    GeneRIF: PKC tethered to AKAP7alpha was less susceptible to
            inhibition from the ATP-competitive inhibitor Go6976 and the
            substrate-competitive inhibitor PKC 20-28, but not the
            activation-competitive inhibitor calphostin C.
REFERENCE   6  (bases 1 to 3150)
  AUTHORS   Brown,R.L., August,S.L., Williams,C.J. and Moss,S.B.
  TITLE     AKAP7gamma is a nuclear RI-binding AKAP
  JOURNAL   Biochem Biophys Res Commun 306 (2), 394-401 (2003)
   PUBMED   12804576
  REMARK    GeneRIF: AKAP7gamma is the first nuclear AKAP to bind RI and may be
            responsible for positioning PKA via RI and/or RII to regulate
            PKA-mediated gene transcription in both somatic cells and oocytes
REFERENCE   7  (bases 1 to 3150)
  AUTHORS   Alto,N.M., Soderling,S.H., Hoshi,N., Langeberg,L.K., Fayos,R.,
            Jennings,P.A. and Scott,J.D.
  TITLE     Bioinformatic design of A-kinase anchoring protein-in silico: a
            potent and selective peptide antagonist of type II protein kinase A
            anchoring
  JOURNAL   Proc Natl Acad Sci U S A 100 (8), 4445-4450 (2003)
   PUBMED   12672969
REFERENCE   8  (bases 1 to 3150)
  AUTHORS   Potet,F., Scott,J.D., Mohammad-Panah,R., Escande,D. and Baro,I.
  TITLE     AKAP proteins anchor cAMP-dependent protein kinase to KvLQT1/IsK
            channel complex
  JOURNAL   Am J Physiol Heart Circ Physiol 280 (5), H2038-H2045 (2001)
   PUBMED   11299204
REFERENCE   9  (bases 1 to 3150)
  AUTHORS   Trotter,K.W., Fraser,I.D., Scott,G.K., Stutts,M.J., Scott,J.D. and
            Milgram,S.L.
  TITLE     Alternative splicing regulates the subcellular localization of
            A-kinase anchoring protein 18 isoforms
  JOURNAL   J Cell Biol 147 (7), 1481-1492 (1999)
   PUBMED   10613906
REFERENCE   10 (bases 1 to 3150)
  AUTHORS   Fraser,I.D., Tavalin,S.J., Lester,L.B., Langeberg,L.K.,
            Westphal,A.M., Dean,R.A., Marrion,N.V. and Scott,J.D.
  TITLE     A novel lipid-anchored A-kinase Anchoring Protein facilitates
            cAMP-responsive membrane events
  JOURNAL   EMBO J 17 (8), 2261-2272 (1998)
   PUBMED   9545239
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL137222.17, DN998807.1,
            AK300587.1 and BC110897.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 29, 2018 this sequence version replaced NM_016377.3.
            
            Summary: This gene encodes a member of the A-kinase anchoring
            protein (AKAP) family, a group of functionally related proteins
            that bind to a regulatory subunit (RII) of cAMP-dependent protein
            kinase A (PKA) and target the enzyme to specific subcellular
            compartments. AKAPs have a common RII-binding domain, but contain
            different targeting motifs responsible for directing PKA to
            distinct intracellular locations. Three alternatively spliced
            transcript variants encoding different isoforms have been
            described.[provided by RefSeq, Apr 2011].
            
            Transcript Variant: This variant (gamma) differs in he 5' UTR and
            has multiple difference in the coding region, compared to variant
            alpha. The encoded isoform (gamma) is longer and has a distinct
            N-terminus, compared to isoform alpha.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.16846.1,
                                           SRR1803615.246646.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            MANE Ensembl match               :: ENST00000431975.7/
                                                ENSP00000405252.2
            RefSeq Select criteria           :: based on manual assertion,
                                                conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-219               AL137222.17        59483-59701
            220-264             DN998807.1         7-51
            265-857             AK300587.1         1-593
            858-858             AL137222.17        93261-93261
            859-1503            AK300587.1         595-1239
            1504-3150           BC110897.1         507-2153
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q23.2"
     gene            1..3150
                     /gene="AKAP7"
                     /gene_synonym="AKAP15; AKAP18"
                     /note="A-kinase anchoring protein 7"
                     /db_xref="GeneID:9465"
                     /db_xref="HGNC:HGNC:377"
                     /db_xref="MIM:604693"
     exon            1..316
                     /gene="AKAP7"
                     /gene_synonym="AKAP15; AKAP18"
                     /inference="alignment:Splign:2.1.0"
     CDS             298..1344
                     /gene="AKAP7"
                     /gene_synonym="AKAP15; AKAP18"
                     /note="isoform gamma is encoded by transcript variant
                     gamma; A-kinase anchor protein 9 kDa; AKAP 18; A-kinase
                     anchor protein 18 kDa; A kinase (PRKA) anchor protein 7"
                     /codon_start=1
                     /product="A-kinase anchoring protein 7 isoform gamma"
                     /protein_id="NP_057461.2"
                     /db_xref="CCDS:CCDS5142.2"
                     /db_xref="GeneID:9465"
                     /db_xref="HGNC:HGNC:377"
                     /db_xref="MIM:604693"
                     /translation="MERPEAGGINSNECENVSRKKKMSEEFEANTMDSLVDMPFATVD
                     IQDDCGITDEPQINLKRSQENEWVKSDQVKKRKKKRKDYQPNYFLSIPITNKEIIKGI
                     KILQNAIIQQDERLAKAMVSDGSFHITLLVMQLLNEDEVNIGIDALLELKPFIEELLQ
                     GKHLTLPFQGIGTFGNQVGFVKLAEGDHVNSLLEIAETANRTFQEKGILVGESRSFKP
                     HLTFMKLSKSPWLRKNGVKKIDPDLYEKFISHRFGEEILYRIDLCSMLKKKQSNGYYH
                     CESSIVIGEKNGGEPDDAELVRLSKRLVENAVLKAVQQYLEETQNKNKPGEGSSVKTE
                     AADQNGNDNENNRK"
     exon            317..448
                     /gene="AKAP7"
                     /gene_synonym="AKAP15; AKAP18"
                     /inference="alignment:Splign:2.1.0"
     exon            449..588
                     /gene="AKAP7"
                     /gene_synonym="AKAP15; AKAP18"
                     /inference="alignment:Splign:2.1.0"
     exon            589..725
                     /gene="AKAP7"
                     /gene_synonym="AKAP15; AKAP18"
                     /inference="alignment:Splign:2.1.0"
     exon            726..886
                     /gene="AKAP7"
                     /gene_synonym="AKAP15; AKAP18"
                     /inference="alignment:Splign:2.1.0"
     exon            887..999
                     /gene="AKAP7"
                     /gene_synonym="AKAP15; AKAP18"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1147
                     /gene="AKAP7"
                     /gene_synonym="AKAP15; AKAP18"
                     /inference="alignment:Splign:2.1.0"
     exon            1148..3150
                     /gene="AKAP7"
                     /gene_synonym="AKAP15; AKAP18"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2543..2548
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AKAP7"
                     /gene_synonym="AKAP15; AKAP18"
                     /note="hexamer: AATAAA"
     polyA_site      2566
                     /gene="AKAP7"
                     /gene_synonym="AKAP15; AKAP18"
     regulatory      3129..3134
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AKAP7"
                     /gene_synonym="AKAP15; AKAP18"
                     /note="hexamer: AATAAA"
     polyA_site      3150
                     /gene="AKAP7"
                     /gene_synonym="AKAP15; AKAP18"
                     /note="major polyA site"
ORIGIN      
        1 ctggcttgtt ccggcgtccg gcctggcatg cgggtgctgc ggctgctgcg gctgccgccg
       61 ccgctgctgc cgctgccgcg ggggctgcgg cttgggaagc tccgcgcttc cgcaggcctg
      121 gcctccgccg cccgggcccc cgcagcctgt cgctggaccc cgcgccggcc cagcgcaccg
      181 ccctcaggcc ccgagccccg ccctggcctc cgcctcggcc tcgcctccag ccccgggacg
      241 cggcccgcca ccgccgctgc cgccagccca gacgcgccgc ccgcatgcgc cgcgaccatg
      301 gagcgccccg aagcgggagg aattaattcc aatgagtgtg aaaatgtatc aagaaaaaag
      361 aaaatgtcag aggaatttga agccaatact atggattctc tggtagacat gccatttgct
      421 actgtagata ttcaggatga ctgtggaatc actgatgaac ctcaaataaa tttgaagaga
      481 agtcaagaaa atgaatgggt caagagtgat caagtaaaga agaggaaaaa aaagagaaaa
      541 gattatcaac ccaactattt cctgtccatt ccaatcacca acaaagagat tataaaagga
      601 attaagatcc tgcagaatgc aataatacaa caagatgagc gactggccaa agcaatggtc
      661 agtgatggtt cctttcatat taccctgctg gtgatgcaat tattaaatga agatgaagta
      721 aacattggta ttgatgctct tttggaattg aaaccattca tagaagaact cctccaggga
      781 aaacatttga ctttgccctt tcaagggatt ggtacttttg gaaatcaggt tggatttgtg
      841 aagctggcag aaggagatca tgtaaactca cttttggaga tagcagagac tgcaaatagg
      901 acatttcaag aaaaaggcat cctggtagga gagagcagaa gttttaaacc tcatttgacc
      961 ttcatgaagt tgtcaaaatc accgtggctc cgtaagaatg gagtgaaaaa aatagatcct
     1021 gatttatatg aaaagtttat cagtcacaga tttggagaag aaatattata tcgcatagat
     1081 ctttgctcca tgctgaagaa aaaacaaagt aatggttatt atcactgtga atcttccatt
     1141 gtgattggtg aaaagaacgg aggggagccc gatgacgctg aactagtaag gctcagtaag
     1201 aggctggtgg agaacgcggt gctcaaggct gtccagcagt atctggagga aacacagaat
     1261 aaaaacaagc cgggggaggg gagctctgtg aaaaccgaag cagctgatca gaatggcaat
     1321 gacaatgaga acaacaggaa atgagcccgg aacgcaggcc cccatgtctc tgtgcaaagc
     1381 ctccctgctt ccctctgctg agtctaggga ctgacttgca gcgtgctgtt taagttaagt
     1441 ttctctggtg caatctgtga agattgccta atacttttca tgatcgatgt gttcgcattg
     1501 ctgaaacaca acagaagaaa aatggagtgc tgggactggc agaggaaatt aattgatgaa
     1561 agaagaatgg cccaagtttc attcgccctc agccacgcac aagggaaagg gaactttggg
     1621 ttatgcctcc tggacgcaaa ttaaaggccg agaaagaggc cttgccatca atggaatact
     1681 gccatttata ttgcttagca gggcatttga ctactttatc tgaggccaga actctcacac
     1741 acagctatca agtgctaagt ttaaaataat cactgttgga attgtcatct gtacaattag
     1801 tccataatgt ttcatgtttg tcctaagtgt gctgttgcta tgcagtgtga tctttattta
     1861 tagtaaatta tgtttcatgt aaatgatata tttttggtga aatgcaacct tttctataaa
     1921 atgtgggcaa cattttaaag tttttttaaa atcctatttt gataagtcag tatgccatat
     1981 ttaatgaaat gttattatat aatttttttt tcttaggcaa gaaacctatt ggaattcgag
     2041 acttaattaa tgaagctttg catcgagaaa cgatgggtct gaagtccaaa gtgaaacaga
     2101 taaaggaact tttattaaag cctgagactc aggccagaat taggagggag ctttttgaag
     2161 gaagacttat taacaacagt aattcagcaa atgacgttga tttcagcaca actttgacat
     2221 aagctctaca ttgcgattgt gacaacatag cttatgaaat cttttcagct tattaagtag
     2281 ctctttggta aacaccaaag aagtttctga tagtgtctgc acaacagcaa accaacattt
     2341 ggtgaggaat tagcaatttc ttgccaaaga aaattgattc tgcccaatta ttttttgagc
     2401 tacacttgtg ttttagaata tctgtttctg taatattgag agttatttta tagaaatgat
     2461 ttcttaatta gctgttgtga gatatttctc gggtccttgc agaaaaaaac atacagactg
     2521 tgaacaaatc attcacaaac agaataaaac agagccaaca acagtatttt aagggtcact
     2581 tgcctcctgt tgacacaatt gttgctaaat caaaagaagc gttgtccagg tgtgtctaca
     2641 tctagtgtta cttttaatga gaatttgaat gtttattgaa caatagtact tgaatgaaca
     2701 tttataaatg taattattgc gatcactggt taagaatgtt ttatatatcc ttataatatt
     2761 tttcactgat caaaatgttg ttctgctttt tcatttctta aggaatacat gtttgggatt
     2821 tttatttttt acgtgtccga agataagctc caggtcttat cgtatccctt gccatctgaa
     2881 cttgtttgca ctgcttctgt ttgaaagagc atcttgaaaa acttccccgg tatgatgatt
     2941 gttggtaaca actttttcta tagtcattga tggagtagat catgatggag gggaaatcac
     3001 tggagatcaa atatgtaaaa tcatttcaaa tataaaatcc agtttactca tggattttag
     3061 ctattttttc actgggtaaa ttatactaca tttatttaca aatgagttta tgcattttca
     3121 tggctcttaa taaacatatt gttttccctt
//
