LOCUS       NM_004857               6487 bp    mRNA    linear   PRI 05-DEC-2024
DEFINITION  Homo sapiens A-kinase anchoring protein 5 (AKAP5), mRNA.
ACCESSION   NM_004857
VERSION     NM_004857.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Tutzauer,J., Serafin,D.S., Schmidt,T., Olde,B., Caron,K.M. and
            Leeb-Lundberg,L.M.F.
  TITLE     G protein-coupled estrogen receptor (GPER)/GPR30 forms a complex
            with the beta1-adrenergic receptor, a membrane-associated guanylate
            kinase (MAGUK) scaffold protein, and protein kinase A anchoring
            protein (AKAP) 5 in MCF7 breast cancer cells
  JOURNAL   Arch Biochem Biophys 752, 109882 (2024)
   PUBMED   38211639
  REMARK    GeneRIF: G protein-coupled estrogen receptor (GPER)/GPR30 forms a
            complex with the beta1-adrenergic receptor, a membrane-associated
            guanylate kinase (MAGUK) scaffold protein, and protein kinase A
            anchoring protein (AKAP) 5 in MCF7 breast cancer cells.
REFERENCE   2  (bases 1 to 6487)
  AUTHORS   Lin,Y.P., Scappini,E., Landaverde,C., Parekh-Glitsch,F.,
            Tucker,C.J., Mirams,G.R. and Parekh,A.B.
  TITLE     Nuanced Interactions between AKAP79 and STIM1 with Orai1 Ca2+
            Channels at Endoplasmic Reticulum-Plasma Membrane Junctions Sustain
            NFAT Activation
  JOURNAL   Mol Cell Biol 42 (11), e0017522 (2022)
   PUBMED   36317924
  REMARK    GeneRIF: Nuanced Interactions between AKAP79 and STIM1 with Orai1
            Ca[2+] Channels at Endoplasmic Reticulum-Plasma Membrane Junctions
            Sustain NFAT Activation.
REFERENCE   3  (bases 1 to 6487)
  AUTHORS   Watson,M., Almeida,T.B., Ray,A., Hanack,C., Elston,R., Btesh,J.,
            McNaughton,P.A. and Stott,K.
  TITLE     Hidden Multivalency in Phosphatase Recruitment by a Disordered AKAP
            Scaffold
  JOURNAL   J Mol Biol 434 (16), 167682 (2022)
   PUBMED   35697294
REFERENCE   4  (bases 1 to 6487)
  AUTHORS   Kar,P., Barak,P., Zerio,A., Lin,Y.P., Parekh,A.J., Watts,V.J.,
            Cooper,D.M.F., Zaccolo,M., Kramer,H. and Parekh,A.B.
  TITLE     AKAP79 Orchestrates a Cyclic AMP Signalosome Adjacent to Orai1 Ca2+
            Channels
  JOURNAL   Function (Oxf) 2 (5), zqab036 (2021)
   PUBMED   34458850
  REMARK    GeneRIF: AKAP79 Orchestrates a Cyclic AMP Signalosome Adjacent to
            Orai1 Ca[2+] Channels.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6487)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 6487)
  AUTHORS   Coghlan,V.M., Perrino,B.A., Howard,M., Langeberg,L.K., Hicks,J.B.,
            Gallatin,W.M. and Scott,J.D.
  TITLE     Association of protein kinase A and protein phosphatase 2B with a
            common anchoring protein
  JOURNAL   Science 267 (5194), 108-111 (1995)
   PUBMED   7528941
REFERENCE   7  (bases 1 to 6487)
  AUTHORS   Carr,D.W., Stofko-Hahn,R.E., Fraser,I.D., Cone,R.D. and Scott,J.D.
  TITLE     Localization of the cAMP-dependent protein kinase to the
            postsynaptic densities by A-kinase anchoring proteins.
            Characterization of AKAP 79
  JOURNAL   J Biol Chem 267 (24), 16816-16823 (1992)
   PUBMED   1512224
REFERENCE   8  (bases 1 to 6487)
  AUTHORS   Carr,D.W., Hausken,Z.E., Fraser,I.D., Stofko-Hahn,R.E. and
            Scott,J.D.
  TITLE     Association of the type II cAMP-dependent protein kinase with a
            human thyroid RII-anchoring protein. Cloning and characterization
            of the RII-binding domain
  JOURNAL   J Biol Chem 267 (19), 13376-13382 (1992)
   PUBMED   1618839
REFERENCE   9  (bases 1 to 6487)
  AUTHORS   Hirsch,A.H., Glantz,S.B., Li,Y., You,Y. and Rubin,C.S.
  TITLE     Cloning and expression of an intron-less gene for AKAP 75, an
            anchor protein for the regulatory subunit of cAMP-dependent protein
            kinase II beta
  JOURNAL   J Biol Chem 267 (4), 2131-2134 (1992)
   PUBMED   1733921
REFERENCE   10 (bases 1 to 6487)
  AUTHORS   Carr,D.W., Stofko-Hahn,R.E., Fraser,I.D., Bishop,S.M., Acott,T.S.,
            Brennan,R.G. and Scott,J.D.
  TITLE     Interaction of the regulatory subunit (RII) of cAMP-dependent
            protein kinase with RII-anchoring proteins occurs through an
            amphipathic helix binding motif
  JOURNAL   J Biol Chem 266 (22), 14188-14192 (1991)
   PUBMED   1860836
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA942424.1, BC057229.1,
            AL122035.6 and BC131516.1.
            
            On Oct 12, 2007 this sequence version replaced NM_004857.2.
            
            Summary: The A-kinase anchor proteins (AKAPs) are a group of
            structurally diverse proteins, which have the common function of
            binding to the regulatory subunit of protein kinase A (PKA) and
            confining the holoenzyme to discrete locations within the cell.
            This gene encodes a member of the AKAP family. The encoded protein
            binds to the RII-beta regulatory subunit of PKA, and also to
            protein kinase C and the phosphatase calcineurin. It is
            predominantly expressed in cerebral cortex and may anchor the PKA
            protein at postsynaptic densities (PSD) and be involved in the
            regulation of postsynaptic events. It is also expressed in T
            lymphocytes and may function to inhibit interleukin-2 transcription
            by disrupting calcineurin-dependent dephosphorylation of NFAT.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK315336.1, SRR1660809.28018.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000394718.4/ ENSP00000378207.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-89                DA942424.1         1-89
            90-985              BC057229.1         4-899
            986-986             AL122035.6         17633-17633         c
            987-1247            BC057229.1         901-1161
            1248-1689           BC131516.1         1480-1921
            1690-6487           AL122035.6         12132-16929         c
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q23.3"
     gene            1..6487
                     /gene="AKAP5"
                     /gene_synonym="AKAP75; AKAP79; H21"
                     /note="A-kinase anchoring protein 5"
                     /db_xref="GeneID:9495"
                     /db_xref="HGNC:HGNC:375"
                     /db_xref="MIM:604688"
     exon            1..115
                     /gene="AKAP5"
                     /gene_synonym="AKAP75; AKAP79; H21"
                     /inference="alignment:Splign:2.1.0"
     exon            116..6487
                     /gene="AKAP5"
                     /gene_synonym="AKAP75; AKAP79; H21"
                     /inference="alignment:Splign:2.1.0"
     CDS             379..1662
                     /gene="AKAP5"
                     /gene_synonym="AKAP75; AKAP79; H21"
                     /note="A-kinase anchoring protein 75/79; AKAP 79; A-kinase
                     anchor protein 79 kDa; cAMP-dependent protein kinase
                     regulatory subunit II high affinity-binding protein; A
                     kinase (PRKA) anchor protein 5"
                     /codon_start=1
                     /product="A-kinase anchor protein 5"
                     /protein_id="NP_004848.3"
                     /db_xref="CCDS:CCDS9764.1"
                     /db_xref="GeneID:9495"
                     /db_xref="HGNC:HGNC:375"
                     /db_xref="MIM:604688"
                     /translation="METTISEIHVENKDEKRSAEGSPGAERQKEKASMLCFKRRKKAA
                     KALKPKAGSEAADVARKCPQEAGASDQPEPTRGAWASLKRLVTRRKRSESSKQQKPLE
                     GEMQPAINAEDADLSKKKAKSRLKIPCIKFPRGPKRSNHSKIIEDSDCSIKVQEEAEI
                     LDIQTQTPLNDQATKAKSTQDLSEGISRKDGDEVCESNVSNSTTSGEKVISVELGLDN
                     GHSAIQTGTLILEEIETIKEKQDVQPQQASPLETSETDHQQPVLSDVPPLPAIPDQQI
                     VEEASNSTLESAPNGKDYESTEIVAEETKPKDTELSQESDFKENGITEEKSKSEESKR
                     MEPIAIIITDTEISEFDVTKSKNVPKQFLISAENEQVGVFANDNGFEDRTSEQYETLL
                     IETASSLVKNAIQLSIEQLVNEMASDDNKINNLLQ"
     regulatory      4859..4864
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AKAP5"
                     /gene_synonym="AKAP75; AKAP79; H21"
                     /note="hexamer: ATTAAA"
     polyA_site      4883
                     /gene="AKAP5"
                     /gene_synonym="AKAP75; AKAP79; H21"
     regulatory      6467..6472
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AKAP5"
                     /gene_synonym="AKAP75; AKAP79; H21"
                     /note="hexamer: AATAAA"
     polyA_site      6487
                     /gene="AKAP5"
                     /gene_synonym="AKAP75; AKAP79; H21"
                     /note="major polyA site"
ORIGIN      
        1 gcagagggcg ccttgctccg ggtgcgaccg cggctccctg gaggcctggg cggcgggccc
       61 cgcgagcgcg ccgccacctc gggagcagtg ttggccaagg accccgacgc cctacgttga
      121 tctttaaata aagcaaaaat ttatccaaag aagggctttc atcaagaaat taaagagaga
      181 aattacaagc catagagaaa ctgggcattt ctatactaga gaaaccacct aaaacaactg
      241 tatggagtaa gatgaaaggt atgaatatgc ctggaagaaa ttttacctag tgtgacattt
      301 ttgctcatct ttttgtcaca aaaggctgct aaggaagaga gaatacagtt ttctagagaa
      361 taagagtgca gtgtaaaaat ggaaaccaca atttcagaaa ttcatgtaga aaacaaggat
      421 gagaagagat cagcagaagg tagtcctggg gctgaaaggc agaaggaaaa ggcatccatg
      481 ctttgcttca agagaagaaa gaaagcagcc aaagcactga agcccaaagc tggctctgaa
      541 gctgctgatg tggcaaggaa gtgtccacaa gaagcaggag cttctgatca gccagagccc
      601 acacgggggg cctgggcctc actcaaacgt cttgtaacac gcaggaaaag gtcagagtct
      661 tcaaagcagc aaaagccatt ggagggtgaa atgcaacctg caataaatgc tgaggatgct
      721 gatctttcta agaaaaaggc aaaatctaga cttaagattc cctgcataaa attcccaaga
      781 gggccaaaaa ggagtaatca ttccaaaatt atagaagact cagactgcag catcaaagtc
      841 caggaagaag ctgaaatttt ggatatacaa acacagaccc cattgaatga tcaggcaaca
      901 aaggctaagt caacccagga tctaagtgaa ggcatctcac ggaaagatgg tgatgaggtc
      961 tgtgaatcaa atgtgagcaa tagcacaact tctggagaga aagtgatttc agtagaactt
     1021 ggattagata atgggcattc tgctattcaa acgggaactc taatccttga agaaattgaa
     1081 acgatcaagg aaaaacaaga tgttcaaccc cagcaagcaa gcccacttga aacttcagaa
     1141 acagaccatc agcagccagt actttctgat gttcctcctt tacctgcaat tccagatcaa
     1201 caaattgtgg aagaagccag taacagtacc ctagaaagtg caccaaatgg aaaagactat
     1261 gaaagtacag agattgtagc tgaagaaact aagccaaaag atactgaatt gagccaagaa
     1321 tcagatttta aagaaaatgg gatcactgaa gagaaatcca aatcagaaga aagcaaaaga
     1381 atggagccaa ttgctattat tattacagac actgaaatca gtgaatttga tgttacaaaa
     1441 tctaaaaatg tccctaagca attcttaatt tcagctgaaa atgagcaagt aggggttttt
     1501 gctaatgata atggttttga ggatagaact tcagaacaat atgaaacact cttaattgaa
     1561 acagcctctt ctctagtcaa gaatgctatt cagttgtcaa tagaacagct ggttaatgaa
     1621 atggcctctg atgataataa aataaacaat cttctacagt gacttactct ccagagtcac
     1681 ggcagaaaaa acaagcttaa tgaagaattc ttttatacat ttgtggcatt tcttactcag
     1741 taacaaatga gagatttatc atctctagaa cttaaaaggt acaattccag cgcagaagtg
     1801 ctaaagatta agtcaagttt ataaaactct accactgaaa tgcagtcact tctggattgg
     1861 aggaagtcag cacagattgc agtgtaaaat gacataacat acagggagtt gctaaaatgg
     1921 catatggaga ttggagtgct ttgggggagg aaggtgtatt tattgagcac ttatggtatg
     1981 ccataagctt tttaatgacc tctgatataa caaagtctgg tttccttttg ataattcagc
     2041 tctgtgtata ggctaacatc acattaagtt tttaaaaact tattttttac agtgcacata
     2101 tatgtttaaa atggtgaata aggtgagcta cttttgtgaa tgtgatttgt aattgttaca
     2161 ttcctaagca gcaggcttaa ctcaaaaaat acattgcatt ttcccaattt cagcagatag
     2221 tgtgcagaat atgcatattg atattaaacg tgagtggttt cctagtctta agtctcttat
     2281 aagcgctatt tggccagggg cagtggctca cgcctataat cccagcactt tgggaggcca
     2341 aggcgggtgg atcagctaag gtcaggagtt cgagaccagc ctggccaatg tggtgaaacc
     2401 ccatctctac tgaaaataca aaaattagct gggcgtggtg gtgcacgcct gtaatcccag
     2461 ctacttggga ggctgaggca ggagaattgc ttgaacccga gaggtggagg ttgcagggag
     2521 ccaagattgc accactgcac tccagtctgg gcaacagagc gagactcctt ctcaaaaaaa
     2581 aaaaaaaaaa aaaagagaac cattcacaaa tccttcaatc aggcaaattg ttactaagtg
     2641 caaaagtgta gaaacaggga gagtgggttt ttcccccgct tttctttctg cctcaacaga
     2701 tctgtatata tccatttctg catggattta tctgtgaaaa gttaaaaaat gaaacagcaa
     2761 aaacaaaaca aggggaatat tattcttcat ccaggagaag ggaaaattcc caagtaaatt
     2821 atcacagata atataggatt catcttgcag aattacttct ttaagagcca tattcatcaa
     2881 cttctctcca aagaggggag agtagattca aagccagcct ggtagtaatg gtcctggaaa
     2941 atcctaataa tctagtgcaa atatttattc tcggccaaaa atgagccatg aatgtgggtg
     3001 aatagaaaag tgaaaagtta ttgctaaagc acattataat ttaaatgtca gtattccttc
     3061 atgtctgtgt gttgagacca gtaaaaagca ttttacatga atattgaaag ttaacttttt
     3121 catcactatt ttcttttctt tttttttttt ttttgagatg gagtctcact ctgtcgccca
     3181 ggctggagtg cagtggcgcg atcttggctc actgcaagct ccacctcctg ggttcacacc
     3241 attcttctgc ctcagcctcc caagtagctg ggactacagg cgcccactac aacgctcggc
     3301 taattttttg tatttttagt agagatgggg tttcaccgtg ttagccagga tggtcttgat
     3361 ctcctgacct catgatcctc ccgcctcggc ctcctaaagt gctgggatta caggcatgag
     3421 ccaccgcacc cggccttcat cactattttc aatccagata tactttacaa aaatgagaag
     3481 ggcttatttg catttttatt tacatattga aagggaagca tctgatctca tcaatgattg
     3541 ccatattttt tgaaaaagta atcctatttc aatctttcct tgtgtgaaat ggccataata
     3601 ccagagtgca cttccctacc tcacaggtta ggattcaaag tgtgtattcc cccattgtgt
     3661 aaattgagtg attgtgttac tacttgaaac agatgaagta ataaagatga agtatgagat
     3721 gtaaggtatt tgttcaagag ctccacttgg aggaaataat gcacattaga tgattttaaa
     3781 tgatctgggt gaattcttgc attgtgtatg gattgcatat agaaagtatt tgctactttg
     3841 tcctacagaa tcatcttcat ttctttctgt aaatacatat tgagacctct aacaaaagaa
     3901 actaatctga cttggaaatt gaaccaaatc atggatatta ttagcaagat tctttgctga
     3961 agtgcaaaaa tatttccccc agattttcct accttcatgc ttataatccg gggaggaggg
     4021 tggaaatcct gagcaagatg aaaagtgtca ttcaaaacac atgacccaag aagtcaattt
     4081 cttttaattg tacaactttt aaacattaaa aacaaaaaag acgagtcatc ttctctgaaa
     4141 acactcttta actcactata tgtggtgcta atggacttgg acagctacca gctatcatat
     4201 ttaaaagtga accattttaa aaacctgact aaggttggtg cattttacag tgtattgaag
     4261 aatcctgccc tcatttactg caaggatgcc tccaagccaa tacacttgca ttgtaaatgt
     4321 ttagtaatct catgaacaag caaaaagtat agtacttagc agttattgga aatattttgg
     4381 gggattttta aaaaaacaga taaatggtaa atatgtgcaa tgaatttttt ggttaagaaa
     4441 gtaaaatttt atctaatact aggtttttac ttttttcact gtggtttgat atatattttt
     4501 ggttagaatg tactgattgt ttttatgata agatgtatat tttacttgca ttcattctta
     4561 agaggtttct gtttttctat tgagaatatc tgttgaatta agtaggattt cagttagaaa
     4621 aaggaattcc gaaattaact ggtctcaatc tgattatatg tgctacatgg ccaaatccta
     4681 ttacagtgaa ttctaaggtg attcaaattg ttttattgtt atagaaatat gcagaaatca
     4741 gatggcttca gagtcataaa atatttttct tgtaatagta tttaagcaat ttgaccttat
     4801 atgcaaacgt taagtgaata taaataagtt ctgtttcaat tcatgtccat ttaacaacat
     4861 taaaataatt attaggaaca ccacatgaat tttctttaca tttgctgtgg tataaattct
     4921 gcttttaatg aatgtttatt tatttattag ccaaaattca gtttctcttt gaacccagaa
     4981 ttttgcaaag ctgggggttg gggaggggga ttattagcta cttgttaact ttttcagcat
     5041 attatcattc ctcttttaag actcaagtat tattcacttt gcttcttaca acccagagaa
     5101 tgtgaaaatt ctcctctatt ttatgtagca ctattcactt aacatttttt caaagcactt
     5161 tataggatat aaaaatagta cccggtaaca tttaagtaaa ggtagcagaa aacaaaagaa
     5221 ctttcaaaca gaaattatgt tccctgccac ctcctccacc tcaaagaata ataagcatgt
     5281 ggcataaatg gagttgttca gaaaagctgt ctgcataatt ttccttcaag gtaatgtatt
     5341 tgacagcatg aagtttgtga tgatcttact tggtctacta ctcagcggag tcttagaatc
     5401 tcttttattt acttctgaga gattgttatt tcaccagtat atgtgaacag gcttgctaaa
     5461 ggcattaaag atatggatca tggaggcacc aaatttggct tctgtatgca atccagcatt
     5521 aacttcatcc atacatttct aacttcatcc ataaatttct aacttcaagt taccttattt
     5581 tttatagtta agaatgtttt tcgaggttta caacagaatt taaaaaataa ttgttaggct
     5641 ttagaatccc actgtttgaa aagatccttt taatgtcaac tttattgaaa tatgtagtct
     5701 actttctaat ttcatctgat aatatagcag gatttgattt ttctgcctgc catttgtgca
     5761 tttaaaaaaa aatcctcatt taagatttag agttctttag gttcaaattc ctatgatgtt
     5821 ttattgggta acatgaactc tcttatacat aattcaaaat ttcaatccat ggtatatttt
     5881 atggtacatt ttgaaaagaa aatttgtaat gctccttagt tacaaaaatt agaaggaagc
     5941 tacataaaat tcaggtgtcc taattaaatt caatttgatt caacacacgt attaggcata
     6001 actcatacct aacactattt gataagggag acaggctccc tgccatccaa gaactcaacc
     6061 tagggagcag tgacaacaga aaccgggttg ggttaaggca aagagccatg gataatgctg
     6121 caacatgagt ggcacagagg ggagtacaca gagagagcaa ttaatttggt gtggggaagg
     6181 tggtgatctg aatacagcag cagtttgaaa gtgttccgtt tttaaataaa cagtatgctt
     6241 attttgattt gtgactaggt gtagaaaaaa gcctgcatag tgttacatat ttatagtagt
     6301 tctttgtaaa aatgttaagt ggatgagcta ttatgaaagt aaaagtttca ttttttctca
     6361 ttaaaattag tactaaatta ctatggatca gatgataata ttaaatagta ctctgtagaa
     6421 aaaaggtttc aagttgaatt aatttatgta ctgattatta atacagaata aatgttatat
     6481 tgactca
//
