LOCUS       NM_005100               8466 bp    mRNA    linear   PRI 17-MAR-2025
DEFINITION  Homo sapiens A-kinase anchoring protein 12 (AKAP12), transcript
            variant 1, mRNA.
ACCESSION   NM_005100
VERSION     NM_005100.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8466)
  AUTHORS   Qasim,H., Rajaei,M., Xu,Y., Reyes-Alcaraz,A., Abdelnasser,H.Y.,
            Stewart,M.D., Lahiri,S.K., Wehrens,X.H.T. and McConnell,B.K.
  TITLE     AKAP12 Upregulation Associates With PDE8A to Accelerate Cardiac
            Dysfunction
  JOURNAL   Circ Res 134 (8), 1006-1022 (2024)
   PUBMED   38506047
  REMARK    GeneRIF: AKAP12 Upregulation Associates With PDE8A to Accelerate
            Cardiac Dysfunction.
REFERENCE   2  (bases 1 to 8466)
  AUTHORS   Li,X., Dong,H., Zheng,Y., Ding,S., Li,Y., Li,H., Huang,H.,
            Zhong,C., Xie,T. and Xu,Y.
  TITLE     AKAP12 inhibits esophageal squamous carcinoma cell proliferation,
            migration, and cell cycle via the PI3K/AKT signaling pathway
  JOURNAL   Mol Cell Probes 72, 101939 (2023)
   PUBMED   37879503
  REMARK    GeneRIF: AKAP12 inhibits esophageal squamous carcinoma cell
            proliferation, migration, and cell cycle via the PI3K/AKT signaling
            pathway.
REFERENCE   3  (bases 1 to 8466)
  AUTHORS   Li,K., Wu,X., Li,Y., Hu,T.T., Wang,W., Gonzalez,F.J. and Liu,W.
  TITLE     AKAP12 promotes cancer stem cell-like phenotypes and activates
            STAT3 in colorectal cancer
  JOURNAL   Clin Transl Oncol 25 (11), 3263-3276 (2023)
   PUBMED   37326825
  REMARK    GeneRIF: AKAP12 promotes cancer stem cell-like phenotypes and
            activates STAT3 in colorectal cancer.
REFERENCE   4  (bases 1 to 8466)
  AUTHORS   Chen,Z., Gong,X., Cheng,C., Fu,Y., Wu,W. and Luo,Z.
  TITLE     Circ_0001777 Affects Triple-negative Breast Cancer Progression
            Through the miR-95-3p/AKAP12 Axis
  JOURNAL   Clin Breast Cancer 23 (2), 143-154 (2023)
   PUBMED   36513585
  REMARK    GeneRIF: Circ_0001777 Affects Triple-negative Breast Cancer
            Progression Through the miR-95-3p/AKAP12 Axis.
REFERENCE   5  (bases 1 to 8466)
  AUTHORS   Shi,Y., Xu,X., He,Z., Tao,H., Chen,Y., Zhang,L., Tao,S., Ding,B.,
            Wang,C. and Yu,L.
  TITLE     AKAP12 and IGFBP4 Are Prognostic Factors for Chronic Lymphocytic
            Leukemia
  JOURNAL   Acta Haematol 146 (6), 474-481 (2023)
   PUBMED   37605556
  REMARK    GeneRIF: AKAP12 and IGFBP4 Are Prognostic Factors for Chronic
            Lymphocytic Leukemia.
REFERENCE   6  (bases 1 to 8466)
  AUTHORS   Shih,M., Lin,F., Scott,J.D., Wang,H.Y. and Malbon,C.C.
  TITLE     Dynamic complexes of beta2-adrenergic receptors with protein
            kinases and phosphatases and the role of gravin
  JOURNAL   J Biol Chem 274 (3), 1588-1595 (1999)
   PUBMED   9880537
REFERENCE   7  (bases 1 to 8466)
  AUTHORS   Sato,N., Kokame,K., Shimokado,K., Kato,H. and Miyata,T.
  TITLE     Changes of gene expression by lysophosphatidylcholine in vascular
            endothelial cells: 12 up-regulated distinct genes including 5 cell
            growth-related, 3 thrombosis-related, and 4 others
  JOURNAL   J Biochem 123 (6), 1119-1126 (1998)
   PUBMED   9604001
REFERENCE   8  (bases 1 to 8466)
  AUTHORS   Nauert,J.B., Klauck,T.M., Langeberg,L.K. and Scott,J.D.
  TITLE     Gravin, an autoantigen recognized by serum from myasthenia gravis
            patients, is a kinase scaffold protein
  JOURNAL   Curr Biol 7 (1), 52-62 (1997)
   PUBMED   9000000
REFERENCE   9  (bases 1 to 8466)
  AUTHORS   Lester,L.B. and Scott,J.D.
  TITLE     Anchoring and scaffold proteins for kinases and phosphatases
  JOURNAL   Recent Prog Horm Res 52, 409-29 (1997)
   PUBMED   9238861
  REMARK    Review article
REFERENCE   10 (bases 1 to 8466)
  AUTHORS   Gordon,T., Grove,B., Loftus,J.C., O'Toole,T., McMillan,R.,
            Lindstrom,J. and Ginsberg,M.H.
  TITLE     Molecular cloning and preliminary characterization of a novel
            cytoplasmic antigen recognized by myasthenia gravis sera
  JOURNAL   J Clin Invest 90 (3), 992-999 (1992)
   PUBMED   1522245
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL356535.19, BC047728.1,
            AB003476.1, U81607.1, AL590413.18 and CN480611.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_005100.3.
            
            Summary: The A-kinase anchor proteins (AKAPs) are a group of
            structurally diverse proteins, which have the common function of
            binding to the regulatory subunit of protein kinase A (PKA) and
            confining the holoenzyme to discrete locations within the cell.
            This gene encodes a member of the AKAP family. The encoded protein
            is expressed in endothelial cells, cultured fibroblasts, and
            osteosarcoma cells. It associates with protein kinases A and C and
            phosphatase, and serves as a scaffold protein in signal
            transduction. This protein and RII PKA colocalize at the cell
            periphery. This protein is a cell growth-related protein.
            Antibodies to this protein can be produced by patients with
            myasthenia gravis. Alternative splicing of this gene results in two
            transcript variants encoding different isoforms. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.242888.1,
                                           SRR1660807.14857.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2142680
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000402676.7/ ENSP00000384537.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-32                AL356535.19        36068-36099
            33-1249             BC047728.1         1-1217
            1250-4426           AB003476.1         857-4033
            4427-6130           U81607.1           4343-6046
            6131-8370           AL590413.18        16929-19168
            8371-8466           CN480611.1         17-112              c
FEATURES             Location/Qualifiers
     source          1..8466
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q25.1"
     gene            1..8466
                     /gene="AKAP12"
                     /gene_synonym="AKAP250; SSeCKS"
                     /note="A-kinase anchoring protein 12"
                     /db_xref="GeneID:9590"
                     /db_xref="HGNC:HGNC:370"
                     /db_xref="MIM:604698"
     exon            1..93
                     /gene="AKAP12"
                     /gene_synonym="AKAP250; SSeCKS"
                     /inference="alignment:Splign:2.1.0"
     exon            94..434
                     /gene="AKAP12"
                     /gene_synonym="AKAP250; SSeCKS"
                     /inference="alignment:Splign:2.1.0"
     CDS             273..5621
                     /gene="AKAP12"
                     /gene_synonym="AKAP250; SSeCKS"
                     /note="isoform 1 is encoded by transcript variant 1;
                     kinase scaffold protein gravin; Src-Suppressed C Kinase
                     Substrate; A-kinase anchor protein, 250kDa; myasthenia
                     gravis autoantigen gravin; A kinase (PRKA) anchor protein
                     12"
                     /codon_start=1
                     /product="A-kinase anchor protein 12 isoform 1"
                     /protein_id="NP_005091.2"
                     /db_xref="CCDS:CCDS5229.1"
                     /db_xref="GeneID:9590"
                     /db_xref="HGNC:HGNC:370"
                     /db_xref="MIM:604698"
                     /translation="MGAGSSTEQRSPEQPPEGSSTPAEPEPSGGGPSAEAAPDTTADP
                     AIAASDPATKLLQKNGQLSTINGVAEQDELSLQEGDLNGQKGALNGQGALNSQEEEEV
                     IVTEVGQRDSEDVSKRDSDKEMATKSAVVHDITDDGQEETPEIIEQIPSSESNLEELT
                     QPTESQANDIGFKKVFKFVGFKFTVKKDKTEKPDTVQLLTVKKDEGEGAAGAGDHKDP
                     SLGAGEAASKESEPKQSTEKPEETLKREQSHAEISPPAESGQAVEECKEEGEEKQEKE
                     PSKSAESPTSPVTSETGSTFKKFFTQGWAGWRKKTSFRKPKEDEVEASEKKKEQEPEK
                     VDTEEDGKAEVASEKLTASEQAHPQEPAESAHEPRLSAEYEKVELPSEEQVSGSQGPS
                     EEKPAPLATEVFDEKIEVHQEEVVAEVHVSTVEERTEEQKTEVEETAGSVPAEELVEM
                     DAEPQEAEPAKELVKLKETCVSGEDPTQGADLSPDEKVLSKPPEGVVSEVEMLSSQER
                     MKVQGSPLKKLFTSTGLKKLSGKKQKGKRGGGDEESGEHTQVPADSPDSQEEQKGESS
                     ASSPEEPEEITCLEKGLAEVQQDGEAEEGATSDGEKKREGVTPWASFKKMVTPKKRVR
                     RPSESDKEDELDKVKSATLSSTESTASEMQEEMKGSVEEPKPEEPKRKVDTSVSWEAL
                     ICVGSSKKRARRGSSSDEEGGPKAMGGDHQKADEAGKDKETGTDGILAGSQEHDPGQG
                     SSSPEQAGSPTEGEGVSTWESFKRLVTPRKKSKSKLEEKSEDSIAGSGVEHSTPDTEP
                     GKEESWVSIKKFIPGRRKKRPDGKQEQAPVEDAGPTGANEDDSDVPAVVPLSEYDAVE
                     REKMEAQQAQKSAEQPEQKAATEVSKELSESQVHMMAAAVADGTRAATIIEERSPSWI
                     SASVTEPLEQVEAEAALLTEEVLEREVIAEEEPPTVTEPLPENREARGDTVVSEAELT
                     PEAVTAAETAGPLGAEEGTEASAAEETTEMVSAVSQLTDSPDTTEEATPVQEVEGGVP
                     DIEEQERRTQEVLQAVAEKVKEESQLPGTGGPEDVLQPVQRAEAERPEEQAEASGLKK
                     ETDVVLKVDAQEAKTEPFTQGKVVGQTTPESFEKAPQVTESIESSELVTTCQAETLAG
                     VKSQEMVMEQAIPPDSVETPTDSETDGSTPVADFDAPGTTQKDEIVEIHEENEVASGT
                     QSGGTEAEAVPAQKERPPAPSSFVFQEETKEQSKMEDTLEHTDKEVSVETVSILSKTE
                     GTQEADQYADEKTKDVPFFEGLEGSIDTGITVSREKVTEVALKGEGTEEAECKKDDAL
                     ELQSHAKSPPSPVEREMVVQVEREKTEAEPTHVNEEKLEHETAVTVSEEVSKQLLQTV
                     NVPIIDGAKEVSSLEGSPPPCLGQEEAVCTKIQVQSSEASFTLTAAAEEEKVLGETAN
                     ILETGETLEPAGAHLVLEEKSSEKNEDFAAHPGEDAVPTGPDCQAKSTPVIVSATTKK
                     GLSSDLEGEKTTSLKWKSDEVDEQVACQEVKVSVAIEDLEPENGILELETKSSKLVQN
                     IIQTAVDQFVRTEETATEMLTSELQTQAHVIKADSQDAGQETEKEGEEPQASAQDETP
                     ITSAKEESESTAVGQAHSDISKDMSEASEKTMTVEVEGSTVNDQQLEEVVLPSEEEGG
                     GAGTKSVPEDDGHALLAERIEKSLVEPKEDEKGDDVDDPENQNSALADTDASGGLTKE
                     SPDTNGPKQKEKEDAQEVELQEGKVHSESDKAITPQAQEELQKQERESAKSELTES"
     exon            435..591
                     /gene="AKAP12"
                     /gene_synonym="AKAP250; SSeCKS"
                     /inference="alignment:Splign:2.1.0"
     exon            592..5633
                     /gene="AKAP12"
                     /gene_synonym="AKAP250; SSeCKS"
                     /inference="alignment:Splign:2.1.0"
     exon            5634..8466
                     /gene="AKAP12"
                     /gene_synonym="AKAP250; SSeCKS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6660..6665
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AKAP12"
                     /gene_synonym="AKAP250; SSeCKS"
                     /note="hexamer: AATAAA"
     polyA_site      6681
                     /gene="AKAP12"
                     /gene_synonym="AKAP250; SSeCKS"
                     /note="major polyA site"
     regulatory      8422..8427
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AKAP12"
                     /gene_synonym="AKAP250; SSeCKS"
                     /note="hexamer: AATAAA"
     regulatory      8438..8443
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AKAP12"
                     /gene_synonym="AKAP250; SSeCKS"
                     /note="hexamer: ATTAAA"
     polyA_site      8443
                     /gene="AKAP12"
                     /gene_synonym="AKAP250; SSeCKS"
                     /note="major polyA site"
     polyA_site      8466
                     /gene="AKAP12"
                     /gene_synonym="AKAP250; SSeCKS"
ORIGIN      
        1 ggagttaaac acaatctgca ggaaagcaaa tggggactcg gactcgctcc tgggcgagct
       61 gaaagtcggc tgcagcagaa gctcctgcct tgggagtttg ccgcgagcgc gtctccttca
      121 ttcgcaggct gggcgcgttc gcagtcggct ggcggcgaag gaaggcgctc tcgggacctc
      181 gcgggcgcgc gtcttttggc tcttgcccct gtccctgcgg cttggggaag gcgtaacccg
      241 gcggctaggc gcgggagaag tgcggaggag ccatgggcgc cgggagctcc accgagcagc
      301 gcagcccgga gcagccgccc gaggggagct ccacgccggc tgagcccgag cccagcggcg
      361 gcggcccctc ggccgaggcg gcgccagaca ccaccgcgga ccccgccatc gctgcctcgg
      421 accccgccac caagctccta cagaagaatg gtcagctgtc caccatcaat ggcgtagctg
      481 agcaagatga gctcagcctc caggagggtg acctaaatgg ccagaaagga gccctgaacg
      541 gtcaaggagc cctaaacagc caggaggaag aagaagtcat tgtcacagag gttggacaga
      601 gagactctga agatgtgagc aaaagagact ccgataaaga gatggctact aagtcagcgg
      661 ttgttcacga catcacagat gatgggcagg aggagacacc cgaaataatc gaacagattc
      721 cttcttcaga aagcaattta gaagagctaa cacaacccac tgagtcccag gctaatgata
      781 ttggatttaa gaaggtgttt aagtttgttg gctttaaatt cactgtgaaa aaggataaga
      841 cagagaagcc tgacactgtc cagctactca ctgtgaagaa agatgaaggg gagggagcag
      901 caggggctgg cgaccacaag gaccccagcc ttggggctgg agaagcagca tccaaagaaa
      961 gcgaacccaa acaatctaca gagaaacccg aagagaccct gaagcgtgag caaagccacg
     1021 cagaaatttc tcccccagcc gaatctggcc aagcagtgga ggaatgcaaa gaggaaggag
     1081 aagagaaaca agaaaaagaa cctagcaagt ctgcagaatc tccgactagt cccgtgacca
     1141 gtgaaacagg atcaaccttc aaaaaattct tcactcaagg ttgggccggc tggcgcaaaa
     1201 agaccagttt caggaagccg aaggaggatg aagtggaagc ttcagagaag aaaaaggaac
     1261 aagagccaga aaaagtagac acagaagaag acggaaaggc agaggttgcc tccgagaaac
     1321 tgaccgcctc cgagcaagcc cacccacagg agccggcaga aagtgcccac gagccccggt
     1381 tatcagctga atatgagaaa gttgagctgc cctcagagga gcaagtcagt ggctcgcagg
     1441 gaccttctga agagaaacct gctccgttgg cgacagaagt gtttgatgag aaaatagaag
     1501 tccaccaaga agaggttgtg gccgaagtcc acgtcagcac cgtggaggag agaaccgaag
     1561 agcagaaaac ggaggtggaa gaaacagcag ggtctgtgcc agctgaagaa ttggttgaaa
     1621 tggatgcaga acctcaggaa gctgaacctg ccaaggagct ggtgaagctc aaagaaacgt
     1681 gtgtttccgg agaggaccct acacagggag ctgacctcag tcctgatgag aaggtgctgt
     1741 ccaaaccccc cgaaggcgtt gtgagtgagg tggaaatgct gtcatcacag gagagaatga
     1801 aggtgcaggg aagtccacta aagaagcttt ttaccagcac tggcttaaaa aagctttctg
     1861 gaaagaaaca gaaagggaaa agaggaggag gagacgagga atcaggggag cacactcagg
     1921 ttccagccga ttctccggac agccaggagg agcaaaaggg cgagagctct gcctcatccc
     1981 ctgaggagcc cgaggagatc acgtgtctgg aaaagggctt agccgaggtg cagcaggatg
     2041 gggaagctga agaaggagct acttccgatg gagagaaaaa aagagaaggt gtcactccct
     2101 gggcatcatt caaaaagatg gtgacgccca agaagcgtgt tagacggcct tcggaaagtg
     2161 ataaagaaga tgagctggac aaggtcaaga gcgctacctt gtcttccacc gagagcacag
     2221 cctctgaaat gcaagaagaa atgaaaggga gcgtggaaga gccaaagccg gaagaaccaa
     2281 agcgcaaggt ggatacctca gtatcttggg aagctttaat ttgtgtggga tcatccaaga
     2341 aaagagcaag gagagggtcc tcttctgatg aggaaggggg accaaaagca atgggaggag
     2401 accaccagaa agctgatgag gccggaaaag acaaagagac ggggacagac gggatccttg
     2461 ctggttccca agaacatgat ccagggcagg gaagttcctc cccggagcaa gctggaagcc
     2521 ctaccgaagg ggagggcgtt tccacctggg agtcatttaa aaggttagtc acgccaagaa
     2581 aaaaatcaaa gtccaagctg gaagagaaaa gcgaagactc catagctggg tctggtgtag
     2641 aacattccac tccagacact gaacccggta aagaagaatc ctgggtctca atcaagaagt
     2701 ttattcctgg acgaaggaag aaaaggccag atgggaaaca agaacaagcc cctgttgaag
     2761 acgcagggcc aacaggggcc aacgaagatg actctgatgt cccggccgtg gtccctctgt
     2821 ctgagtatga tgctgtagaa agggagaaaa tggaggcaca gcaagcccaa aaaagcgcag
     2881 agcagcccga gcagaaggca gccactgagg tgtccaagga gctcagcgag agtcaggttc
     2941 atatgatggc agcagctgtc gctgacggga cgagggcagc taccattatt gaagaaaggt
     3001 ctccttcttg gatatctgct tcagtgacag aacctcttga acaagtagaa gctgaagccg
     3061 cactgttaac tgaggaggta ttggaaagag aagtaattgc agaagaagaa ccccccacgg
     3121 ttactgaacc tctgccagag aacagagagg cccggggcga cacggtcgtt agtgaggcgg
     3181 aattgacccc cgaagctgtg acagctgcag aaactgcagg gccattgggt gccgaagaag
     3241 gaaccgaagc atctgctgct gaagagacca cagaaatggt gtcagcagtc tcccagttaa
     3301 ccgactcccc agacaccaca gaggaggcca ctccggtgca ggaggtggaa ggtggcgtac
     3361 ctgacataga agagcaagag aggcggactc aagaggtcct ccaggcagtg gcagaaaaag
     3421 tgaaagagga atcccagctg cctggcaccg gtgggccaga agatgtgctt cagcctgtgc
     3481 agagagcaga ggcagaaaga ccagaagagc aggctgaagc gtcgggtctg aagaaagaga
     3541 cggatgtagt gttgaaagta gatgctcagg aggcaaaaac tgagcctttt acacaaggga
     3601 aggtggtggg gcagaccacc ccagaaagct ttgaaaaagc tcctcaagtc acagagagca
     3661 tagagtccag tgagcttgta accacttgtc aagccgaaac cttagctggg gtaaaatcac
     3721 aggagatggt gatggaacag gctatccccc ctgactcggt ggaaacccct acagacagtg
     3781 agactgatgg aagcaccccc gtagccgact ttgacgcacc aggcacaacc cagaaagacg
     3841 agattgtgga aatccatgag gagaatgagg tcgcatctgg tacccagtca gggggcacag
     3901 aagcagaggc agttcctgca cagaaagaga ggcctccagc accttccagt tttgtgttcc
     3961 aggaagaaac taaagaacaa tcaaagatgg aagacactct agagcataca gataaagagg
     4021 tgtcagtgga aactgtatcc attctgtcaa agactgaggg gactcaagag gctgaccagt
     4081 atgctgatga gaaaaccaaa gacgtaccat ttttcgaagg acttgagggg tctatagaca
     4141 caggcataac agtcagtcgg gaaaaggtca ctgaagttgc ccttaaaggt gaagggacag
     4201 aagaagctga atgtaaaaag gatgatgctc ttgaactgca gagtcacgct aagtctcctc
     4261 catcccccgt ggagagagag atggtagttc aagtcgaaag ggagaaaaca gaagcagagc
     4321 caacccatgt gaatgaagag aagcttgagc acgaaacagc tgttaccgta tctgaagagg
     4381 tcagtaagca gctcctccag acagtgaatg tgcccatcat agatggggca aaggaagtca
     4441 gcagtttgga aggaagccct cctccctgcc taggtcaaga ggaggcagta tgcaccaaaa
     4501 ttcaagttca gagctctgag gcatcattca ctctaacagc ggctgcagag gaggaaaagg
     4561 tcttaggaga aactgccaac attttagaaa caggtgaaac gttggagcct gcaggtgcac
     4621 atttagttct ggaagagaaa tcctctgaaa aaaatgaaga ctttgccgct catccagggg
     4681 aagatgctgt gcccacaggg cccgactgtc aggcaaaatc gacaccagtg atagtatctg
     4741 ctactaccaa gaaaggctta agttccgacc tggaaggaga gaaaaccaca tcactgaagt
     4801 ggaagtcaga tgaagtcgat gagcaggttg cttgccagga ggtcaaagtg agtgtagcaa
     4861 ttgaggattt agagcctgaa aatgggattt tggaacttga gaccaaaagc agtaaacttg
     4921 tccaaaacat catccagaca gccgttgacc agtttgtacg tacagaagaa acagccaccg
     4981 aaatgttgac gtctgagtta cagacacaag ctcacgtgat aaaagctgac agccaggacg
     5041 ctggacagga aacggagaaa gaaggagagg aacctcaggc ctctgcacag gatgaaacac
     5101 caattacttc agccaaagag gagtcagagt caaccgcagt gggacaagca cattctgata
     5161 tttccaaaga catgagtgaa gcctcagaaa agaccatgac tgttgaggta gaaggttcca
     5221 ctgtaaatga tcagcagctg gaagaggtcg tcctcccatc tgaggaagag ggaggtggag
     5281 ctggaacaaa gtctgtgcca gaagatgatg gtcatgcctt gttagcagaa agaatagaga
     5341 agtcactagt tgaaccgaaa gaagatgaaa aaggtgatga tgttgatgac cctgaaaacc
     5401 agaactcagc cctggctgat actgatgcct caggaggctt aaccaaagag tccccagata
     5461 caaatggacc aaaacaaaaa gagaaggagg atgcccagga agtagaattg caggaaggaa
     5521 aagtgcacag tgaatcagat aaagcgatca caccccaagc acaggaggag ttacagaaac
     5581 aagagagaga atctgcaaag tcagaactta cagaatctta aaacatcatg cagttaaact
     5641 cattgtctgt ttggaagacc agaatgtgaa gacaagtagt agaagaaaat gaatgctgct
     5701 gctgagactg aagaccagta tttcagaact ttgagaattg gagagcaggc acatcaactg
     5761 atctcatttc tagagagccc ctgacaatcc tgaggcttca tcaggagcta gagccattta
     5821 acatttcctc tttccaagac caacctacaa ttttcccttg ataaccatat aaattctgat
     5881 ttaaggtcct aaattcttaa cctggaactg gagttggcaa tacctagttc tgcttctgaa
     5941 actggagtat cattctttac atatttatat gtatgtttta agtagtcctc ctgtatctat
     6001 tgtatatttt tttcttaatg tttaaggaaa tgtgcaggat actacatgct ttttgtatca
     6061 cacagtatat gatggggcat gtgccatagt gcaggcttgg ggagctttaa gcctcagtta
     6121 tataacccac gaaaaacaga gcctcctaga tgtaacattc ctgatcaagg tacaattctt
     6181 taaaattcac taatgattga ggtccatatt tagtggtact ctgaaattgg tcactttcct
     6241 attacacgga gtgtgctaaa actaaaaagc attttgaaac atacagaatg ttctattgtc
     6301 attgggaaat ttttctttct aacccagtgg aggttagaaa gaagttatat tctggtagca
     6361 aattaacttt acatcctttt tcctacttgt tatggttgtt tggaccgata agtgtgctta
     6421 atcctgaggc aaagtagtga atatgtttta tatgttatga agaaaagaat tgttgtaagt
     6481 ttttgattct actcttatat gctggactgc attcacacat ggcatgaaat aagtcaggtt
     6541 ctttacaaat ggtattttga tagatactgg attgtgtttg tgccatattt gtgccattct
     6601 tttaagaaca atgttgcaac acattcattt ggataagttg tgatttgacg actgatttaa
     6661 ataaaatatt tgcttcactt agatttgctg gttttattag ataccaggaa gcctgggaca
     6721 tatgtgtacc acattagaat tctaaagata atgattgata agctagaact ttctgatgta
     6781 gtcattacat gaaacccctt gtcactggtt tgtgtgttca gaggaagcca tggccgagat
     6841 agctttcctg aaataaacca gtagcttttc agattgacgt tcttgctaca attgtaccat
     6901 ctggtaattc ctgaaaatgt caattttttt gtgttaatat ttttggtttc aaacaataac
     6961 aaatgtctct agaaagaaat tttaagaaag cttaattaat agtaaaaatg cctttcctga
     7021 aataatcttg gaaaattttt taaatgtcaa aatgatgagt catgctaata cattgagggt
     7081 ttgttttttt gtttgtttgt ttgtttgttt ttgagacaga gtttcgctct tgttgcccag
     7141 gctggagtgc aatggcacga tctcagctca ccgcaacctc cacctcccgg attccagcga
     7201 ttctcctgcc tcagcctaca ttaagggttt tgtcagacaa ttgtcacacg aagaatagtg
     7261 tcacttatct actcttgaca cacagaactg gcctggcata tagctttcca gattttactc
     7321 aaacttggta ctccagtttg aaaatttaaa ttttgactgc tgattagctg gaaagcctag
     7381 ttttaatgga aagaaagttt gcttttaaaa ctgaaagtag tttctttttg ctaacaaatc
     7441 taacttcata cataattggc catattagta aaacacctca tgatagcagt gtatatatag
     7501 tcttgtttgt agttggaagt catcttttag gagttattct caaatatata taatagctac
     7561 ccatgcatca ttattaaaat ccccaaattc aaaaaacctc tgatatatat atataatttt
     7621 tttttttttt tttttttggc caactgagat tgaaatccaa gtgctggttt ctagttctga
     7681 acatcaacta aagagttttg gaaatgacag caatttataa caagttcata ttgacttcct
     7741 ctctatggca ggaagacatt ctgtgctgtt ttgaacagat taaagatttg tgtagtttgt
     7801 gggaaattga cgtttttgtt taaattccac ccgcgtttgt cttttcctac cacctgtggc
     7861 caggtgctcg ctggccatca cagttgcgat tccatgagta gctgctttat gactgctttt
     7921 tgtactatct ggatgtgccc agagttactt ctgtacaagc tctgtatcta tgtccgttga
     7981 gaacattatt ttaacaagaa gaacaccaac agtagcatga aatataatac tgttttataa
     8041 ttctaaagct gctgttaatt tatgaagtac ataataatct aatgtaaact gcagaagtca
     8101 gagcaagtgc ctacattttg ttatttttgg cattactaca gagccatgta caatagaaag
     8161 caatgcaaga cttgtaaact ctcaccactt cttgtaatat caaatgttcc ccctcaggtt
     8221 attttgctta tggtacccat gagttgcctc tctctgtaca tagataaatt gttccaatat
     8281 tttcctttga tgtttggaac tacagatagt caagggctgg aaattttagt tttcaatata
     8341 agcttccagc ttagcaatta cctctagtcg aagacaatat ttgattccta gttctgtttg
     8401 gggcaaattt tcatttatct aaataaaatg caatctaatt aaatgccatg gattttcttt
     8461 ctgtaa
//
