LOCUS       NM_001145128            6317 bp    mRNA    linear   PRI 13-FEB-2025
DEFINITION  Homo sapiens adenylate kinase 9 (AK9), transcript variant 1, mRNA.
ACCESSION   NM_001145128
VERSION     NM_001145128.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Hua,T., Zhang,C., Fu,Y., Qin,N., Liu,S., Chen,C., Gong,L., Ma,H.,
            Ding,Y., Wei,X., Jin,C., Jin,C., Zhu,M., Zhang,E., Dai,J. and Ma,H.
  TITLE     Integrative analyses of N6-methyladenosine-associated
            single-nucleotide polymorphisms (m6A-SNPs) identify tumor
            suppressor gene AK9 in lung cancer
  JOURNAL   Mol Carcinog 63 (3), 538-548 (2024)
   PUBMED   38051288
  REMARK    GeneRIF: Integrative analyses of N6-methyladenosine-associated
            single-nucleotide polymorphisms (m6A-SNPs) identify tumor
            suppressor gene AK9 in lung cancer.
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   Yang,H.W., Lee,S., Berry,B.C., Yang,D., Zheng,S., Carroll,R.S.,
            Park,P.J. and Johnson,M.D.
  TITLE     A role for mutations in AK9 and other genes affecting ependymal
            cells in idiopathic normal pressure hydrocephalus
  JOURNAL   Proc Natl Acad Sci U S A 120 (51), e2300681120 (2023)
   PUBMED   38100419
  REMARK    GeneRIF: A role for mutations in AK9 and other genes affecting
            ependymal cells in idiopathic normal pressure hydrocephalus.
REFERENCE   3  (bases 1 to 6317)
  AUTHORS   Sha,Y., Liu,W., Li,S., Osadchuk,L.V., Chen,Y., Nie,H., Gao,S.,
            Xie,L., Qin,W., Zhou,H. and Li,L.
  TITLE     Deficiency in AK9 causes asthenozoospermia and male infertility by
            destabilising sperm nucleotide homeostasis
  JOURNAL   EBioMedicine 96, 104798 (2023)
   PUBMED   37713809
  REMARK    GeneRIF: Deficiency in AK9 causes asthenozoospermia and male
            infertility by destabilising sperm nucleotide homeostasis.
            Erratum:[EBioMedicine. 2024 Jan;99:104957. doi:
            10.1016/j.ebiom.2023.104957.. PMID: 38171079]
REFERENCE   4  (bases 1 to 6317)
  AUTHORS   Lam,C.W., Wong,K.S., Leung,H.W. and Law,C.Y.
  TITLE     Limb girdle myasthenia with digenic RAPSN and a novel disease gene
            AK9 mutations
  JOURNAL   Eur J Hum Genet 25 (2), 192-199 (2017)
   PUBMED   27966543
  REMARK    GeneRIF: AK2 identified as a novel disease modifier in
            RASPN-associated limb girdle myasthenia.
REFERENCE   5  (bases 1 to 6317)
  AUTHORS   Amiri,M., Conserva,F., Panayiotou,C., Karlsson,A. and Solaroli,N.
  TITLE     The human adenylate kinase 9 is a nucleoside mono- and diphosphate
            kinase
  JOURNAL   Int J Biochem Cell Biol 45 (5), 925-931 (2013)
   PUBMED   23416111
REFERENCE   6  (bases 1 to 6317)
  AUTHORS   Van Rompay,A.R., Johansson,M. and Karlsson,A.
  TITLE     Identification of a novel human adenylate kinase. cDNA cloning,
            expression analysis, chromosome localization and characterization
            of the recombinant protein
  JOURNAL   Eur J Biochem 261 (2), 509-517 (1999)
   PUBMED   10215863
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL133472.12, AL121788.17,
            AL139391.11 and AL109947.19.
            
            On Aug 14, 2020 this sequence version replaced NM_001145128.2.
            
            Summary: The protein encoded by this gene catalyzes the
            interconversion of nucleosides, possessing both nucleoside
            monophosphate and diphosphate kinase activities. The encoded
            protein uses these interconversions to maintain nucleoside
            homeostasis. [provided by RefSeq, Jul 2016].
            
            Transcript Variant: This variant (1) is the longest transcript and
            encodes the longest isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The splice pattern of this variant is inferred
            from partial transcripts and orthologous data, and has not been
            experimentally confirmed.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000424296.7/ ENSP00000410186.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-56                AL133472.12        18551-18606         c
            57-184              AL133472.12        3033-3160           c
            185-248             AL133472.12        1602-1665           c
            249-301             AL121788.17        134498-134550       c
            302-398             AL121788.17        134302-134398       c
            399-511             AL121788.17        124934-125046       c
            512-697             AL121788.17        121611-121796       c
            698-826             AL121788.17        119139-119267       c
            827-901             AL121788.17        106997-107071       c
            902-1000            AL121788.17        103901-103999       c
            1001-1140           AL121788.17        95567-95706         c
            1141-1321           AL121788.17        95306-95486         c
            1322-1466           AL121788.17        81475-81619         c
            1467-1562           AL121788.17        76768-76863         c
            1563-1676           AL121788.17        76566-76679         c
            1677-1760           AL121788.17        74393-74476         c
            1761-1909           AL121788.17        72748-72896         c
            1910-2066           AL121788.17        48299-48455         c
            2067-2181           AL121788.17        47506-47620         c
            2182-2258           AL121788.17        41933-42009         c
            2259-2411           AL121788.17        35825-35977         c
            2412-2501           AL121788.17        27139-27228         c
            2502-2702           AL121788.17        26463-26663         c
            2703-2818           AL121788.17        25980-26095         c
            2819-3031           AL121788.17        12473-12685         c
            3032-3292           AL121788.17        8250-8510           c
            3293-3417           AL121788.17        4430-4554           c
            3418-3637           AL139391.11        3546-3765           c
            3638-3700           AL109947.19        128166-128228       c
            3701-3913           AL109947.19        115585-115797       c
            3914-4132           AL109947.19        115012-115230       c
            4133-4346           AL109947.19        113379-113592       c
            4347-4548           AL109947.19        108334-108535       c
            4549-4695           AL109947.19        105809-105955       c
            4696-4916           AL109947.19        105482-105702       c
            4917-5113           AL109947.19        98199-98395         c
            5114-5283           AL109947.19        96951-97120         c
            5284-5382           AL109947.19        96620-96718         c
            5383-5485           AL109947.19        94493-94595         c
            5486-5600           AL109947.19        93136-93250         c
            5601-6317           AL109947.19        92010-92726         c
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q21"
     gene            1..6317
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /note="adenylate kinase 9"
                     /db_xref="GeneID:221264"
                     /db_xref="HGNC:HGNC:33814"
                     /db_xref="MIM:615358"
     exon            1..56
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            57..184
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     CDS             68..5803
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /EC_number="2.7.4.6"
                     /EC_number="2.7.4.4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     adenylate kinase domain containing 2; adenylate kinase
                     domain containing 1"
                     /codon_start=1
                     /product="adenylate kinase 9 isoform 1"
                     /protein_id="NP_001138600.2"
                     /db_xref="CCDS:CCDS55048.1"
                     /db_xref="GeneID:221264"
                     /db_xref="HGNC:HGNC:33814"
                     /db_xref="MIM:615358"
                     /translation="MTSQEKTEEYPFADIFDEDETERNFLLSKPVCFVVFGKPGVGKT
                     TLARYITQAWKCIRVEALPILEEQIAAETESGVMLQSMLISGQSIPDELVIKLMLEKL
                     NSPEVCHFGYIITEIPSLSQDAMTTLQQIELIKNLNLKPDVIINIKCPDYDLCQRISG
                     QRQHNNTGYIYSRDQWDPEVIENHRKKKKEAQKDGKGEEEEEEEEQEEEEAFIAEMQM
                     VAEILHHLVQRPEDYLENVENIVKLYKETILQTLEEVMAEHNPQYLIELNGNKPAEEL
                     FMIVMDRLKYLNLKRAAILTKLQGAEEEINDTMENDELFRTLASYKLIAPRYRWQRSK
                     WGRTCPVNLKDGNIYSGLPDYSVSFLGKIYCLSSEEALKPFLLNPRPYLLPPMPGPPC
                     KVFILGPQYSGKTTLCNMLAENYKGKVVDYAQLVQPRFDKARETLVENTIAEATAAAI
                     KVVKEKLLRELQARKQAETALREFQRQYEKMEFGVFPMEATHSSIDEEGYIQGSQRDR
                     GSSLVDTEEAKTKSENVLHDQAAKVDKDDGKETGETFTFKRHSQDASQDVKLYSDTAP
                     TEDLIEEVTADHPEVVTMIEETIKMSQDINFEQPYEKHAEILQEVLGEVMEENKDRFP
                     GAPKYGGWIVDNCPIVKELWMALIKKGIIPDLVIYLSDTENNGKCLFNRIYLQKKSEI
                     DSKILERLLEELQKKKKEEEEARKATEEELRLEEENRRLLELMKVKAKEAEETDNEDE
                     EEIEGDELEVHEEPEASHDTRGSWLPEEFEASEVPETEPEAVSEPIEETTVETEIPKG
                     SKEGLEIEKLSETVVLPEFPEDSYPDVPEMEPFKEKIGSFIILWKQLEATISEAYIKI
                     LNLEIADRTPQELLQKVVETMEKPFQYTAWELTGEDYEEETEDYQTEAEVDEELEEEE
                     EEEGEDKMKERKRHLGDTKHFCPVVLKENFILQPGNTEEAAKYREKIYYFSSAEAKEK
                     FLEHPEDYVAHEEPLKAPPLRICLVGPQGSGKTMCGRQLAEKLNIFHIQFEEVLQEKL
                     LLKTEKKVGPEFEEDSENEQAAKQELEELAIQANVKVEEENTKKQLPEVQLTEEEEVI
                     KSSLMENEPLPPEILEVILSEWWLKEPIRSTGFILDGFPRYPEEAQFLGDRGFFPDAA
                     VFIQVDDQDIFDRLLPAQIEKWKLKQKKKLERKKLIKDMKAKIRVDTIAKRRAELILE
                     RDKKRRENVVRDDEEISEEELEEDNDDIENILEDEFPKDEEEMSGEEDEEQETDAIER
                     LRGELGEKFEADTHNLQIIQDELERYLIPIISINGARRNHIVQYTLNMKLKPLVENRA
                     SIFEKCHPIPAPLAQKMLTFTYKYISSFGYWDPVKLSEGETIKPVENAENPIYPVIHR
                     QYIYFLSSKETKEKFMKNPIKYIRQPKPKPTVPIRIIIVGPPKSGKTTVAKKITSEYG
                     LKHLSIGGALRYVLNNHPETELALMLNWHLHKGMTAPDELAIQALELSLMESVCNTAG
                     VVIDGYPVTKHQMNLLEARSIIPMVIFELSVPSKEIFKRLLLEKENEQRLPYPLHNSA
                     QIVAVNNVKYRKNIGEIRQYYQEQHQNWYVIDGFHSKWWVWNEVIKNVQMVNKYMQTY
                     LERIKAGKAACIDKLCITPQELLSRLGEFEQFCPVSLAESQELFDCSATDSLEFAAEF
                     RGHYYKMSSQEKLNKFLENPELYVPPLAPHPLPSADMIPKRLTLSELKSRFPKCAELQ
                     GYCPVTYKDGNQRYEALVPGSINYALEYHNRIYICENKEKLQKFLRSPLKYWEQKLPH
                     KLPPLREPILLTSLPLPGYLEQGIATSLIKAMNAAGCLKPKFPFLSIRRSALLYIALH
                     LKAFNPKGSEYTRKKYKKKMEQFMESCELITYLGAKMTRKYKEPQFRAIDFDHKLKTF
                     LSLRNIDPING"
     exon            185..248
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            249..301
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            302..398
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            399..511
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            512..697
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            698..826
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            827..901
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            902..1000
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1140
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            1141..1321
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            1322..1466
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            1467..1562
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            1563..1676
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            1677..1760
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            1761..1909
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            1910..2066
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            2067..2181
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            2182..2258
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            2259..2411
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            2412..2501
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            2502..2702
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            2703..2818
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            2819..3031
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            3032..3292
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            3293..3417
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            3418..3637
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            3638..3700
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            3701..3913
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            3914..4132
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            4133..4346
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            4347..4548
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            4549..4695
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            4696..4916
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            4917..5113
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            5114..5283
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            5284..5382
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            5383..5485
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            5486..5600
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     exon            5601..6317
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6050..6055
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /note="hexamer: AATAAA"
     polyA_site      6074
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /note="major polyA site"
     regulatory      6293..6298
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /note="hexamer: AATAAA"
     polyA_site      6317
                     /gene="AK9"
                     /gene_synonym="AK 9; AKD1; AKD2; C6orf199; C6orf224;
                     dJ70A9.1; SPGF89"
                     /note="major polyA site"
ORIGIN      
        1 agaggctccc gggacctgcg tgctgccgag agaggaagcg aagggcacca tctttgtatt
       61 ttgtgtcatg acttctcaag agaagacaga agagtatcct tttgcagata tatttgatga
      121 agatgaaact gaaaggaatt ttttgttgtc caaacctgtt tgctttgttg tatttgggaa
      181 accaggtgtt gggaaaacaa cattagcccg ttacataaca caggcatgga aatgtattcg
      241 tgttgaagct ttgccaattt tagaagaaca gattgctgct gaaaccgaat caggagttat
      301 gttgcaatca atgttgatca gcggtcaaag cattccagat gaacttgtca taaagctaat
      361 gttggagaag ctcaactccc cagaagtctg tcactttggt tatattatca ctgaaatacc
      421 atcactttca caggatgcca tgactacctt acagcaaata gaattaatta aaaacttaaa
      481 cctgaaacct gatgttataa tcaatataaa gtgtcctgac tatgatttgt gccagagaat
      541 ttctgggcaa agacagcaca ataatacggg atacatatac agtagagacc agtgggatcc
      601 tgaagtcatt gagaatcata ggaaaaagaa gaaagaagcc caaaaggacg gaaaaggaga
      661 agaggaagaa gaggaagaag agcaagaaga agaagaggca tttattgccg aaatgcagat
      721 ggtggctgaa attcttcatc atctagttca gaggcctgaa gattatttgg aaaatgttga
      781 aaacattgtt aagctttata aggaaacaat tctccaaact ttagaagaag taatggctga
      841 acacaatccc cagtatctca ttgagctaaa tggaaataaa ccagcagagg agctctttat
      901 gattgttatg gatcgactta aatatctgaa cctaaaaaga gcagctattc taaccaaact
      961 tcagggtgca gaggaagaaa ttaatgacac aatggaaaat gatgagctat ttcgtactct
     1021 tgcatcttat aaacttattg caccaagata cagatggcaa agaagtaaat ggggacgtac
     1081 atgtcctgtg aatttaaaag atggtaacat ttattcagga ttaccagatt attctgtgag
     1141 ttttctaggt aaaatctact gtctttcatc agaagaagca ttaaaaccat ttttgttgaa
     1201 cccacgtccc tatctgcttc cacctatgcc aggaccacca tgtaaagtat tcatacttgg
     1261 acctcaatat tcagggaaaa caacactttg caatatgctt gcagaaaatt acaaaggaaa
     1321 ggtagtcgac tatgcccaac ttgttcagcc acgttttgat aaagcccgtg aaacattagt
     1381 agaaaatacc atagctgagg ccactgcagc agcaattaaa gttgtgaaag aaaagcttct
     1441 cagggaactg caagctagaa aacaagctga aacagcttta agagaatttc aaaggcaata
     1501 tgaaaaaatg gagtttggag tattcccaat ggaggcaaca cactcatcaa ttgatgaaga
     1561 agggtacatt caaggctccc aaagggacag aggcagctct ttagtggaca ccgaagaagc
     1621 caaaacaaag tcagaaaatg tcctccatga tcaagctgct aaagttgata aagatgatgg
     1681 aaaagaaact ggtgaaacat tcacatttaa aaggcattct caagatgcta gtcaagatgt
     1741 aaagttgtat tcagatacag ccccaacaga agacttgata gaagaggtaa ctgcagatca
     1801 tccagaggtt gtgaccatga ttgaagagac tataaaaatg tcacaggata taaactttga
     1861 acagccatat gaaaaacatg ctgaaatctt acaggaagtc cttggagagg taatggaaga
     1921 aaacaaggat aggtttcctg gtgccccaaa atatggaggc tggattgtgg acaactgccc
     1981 tattgtaaaa gaattgtgga tggccttaat caagaaagga attatacctg atttggtcat
     2041 ctatttatca gatacagaaa acaatggaaa atgtttattt aatagaatat atttacagaa
     2101 gaaatctgaa attgactcta agattttaga aagattatta gaagaactac aaaagaaaaa
     2161 aaaagaagaa gaagaagcaa gaaaagccac agaagaggaa ttgagactcg aagaagaaaa
     2221 tcgaaggcta ctggaactta tgaaagtgaa ggcaaaagaa gctgaagaga ctgataatga
     2281 ggatgaagag gagattgaag gtgatgagtt ggaagttcac gaagagcctg aggcatctca
     2341 cgatacccga gggtcatggt tacctgagga gtttgaagca tctgaggtcc ctgaaactga
     2401 gcctgaagca gtatctgagc ctatcgagga aactacagtg gaaacagaaa tcccaaaagg
     2461 atccaaagag ggcctggaaa ttgaaaaatt atctgaaaca gttgtactac ctgagtttcc
     2521 agaagactct tatcctgatg ttcccgaaat ggagccattt aaagagaaga ttggttcttt
     2581 catcatcctc tggaaacagc tagaagcaac aattagtgag gcttacatta aaattttaaa
     2641 cttggagatt gctgacagaa ctccacagga attacttcaa aaagtagttg agactatgga
     2701 aaaaccattt caatatactg catgggagtt aactggggaa gattatgagg aagaaacaga
     2761 agactaccag actgaagcag aggttgatga ggagctagag gaagaggaag aggaagaggg
     2821 tgaagataaa atgaaggaga gaaagaggca tttgggagac acaaaacact tttgtccggt
     2881 ggtcctcaaa gaaaacttca tcctgcaacc aggaaacaca gaagaagcag ccaagtatcg
     2941 agaaaagatc tactactttt caagtgctga ggctaaagaa aagtttttgg agcatcctga
     3001 ggattatgtg gctcatgaag aaccattgaa ggctcctcca ttaagaatat gccttgtcgg
     3061 cccccagggc tctggcaaaa ctatgtgtgg aagacagttg gcagaaaaat taaacatttt
     3121 tcacattcag tttgaagaag ttcttcaaga aaaactacta ctcaaaactg aaaagaaagt
     3181 gggacctgaa tttgaggaag attctgagaa cgagcaagct gccaaacaag aacttgaaga
     3241 gcttgcaatt caggccaatg tcaaagttga ggaagaaaat acaaaaaagc agcttccaga
     3301 agtacaactt acagaagaag aagaagtaat caaatcaagt ctaatggaaa atgagccctt
     3361 gcctcctgaa attcttgaag taattctttc tgagtggtgg cttaaggaac caatacgttc
     3421 cacaggtttt atattagatg gtttcccacg atatccagaa gaggcccagt ttttgggaga
     3481 tcgtggattt ttcccagatg cagctgtttt tatacaagtt gatgatcaag atatttttga
     3541 tcgcctcctt cctgcccaaa ttgaaaagtg gaaactaaaa caaaagaaga aattagaaag
     3601 gaagaaactg atcaaagaca tgaaggcaaa aatcagggtt gatacgattg ctaaaagaag
     3661 ggctgaactt atattagaga gagataaaaa aaggagggag aatgttgtta gagatgatga
     3721 agagattagt gaggaagaac ttgaagaaga caatgatgat attgaaaaca tccttgaaga
     3781 tgagtttcca aaagatgagg aagagatgag tggcgaggaa gatgaagaac aggaaactga
     3841 tgcaattgag cgcctgagag gtgaactagg agaaaaattt gaagcagata cacataattt
     3901 acaaataata caggatgaac ttgagaggta tttgatacca ataatttcca ttaatggagc
     3961 tcggagaaat cacattgtac aatatacatt gaatatgaaa ctgaaaccac tggtggaaaa
     4021 tcgtgcaagc atttttgaga aatgtcatcc aataccagca ccccttgccc agaaaatgct
     4081 cacctttacc tacaagtata taagctcatt cggctattgg gaccctgtaa agctaagtga
     4141 aggagagaca atcaagccag ttgaaaatgc agagaatcca atttatcctg taatccatcg
     4201 tcagtatatt tattttttat ctagtaaaga aacaaaagaa aaatttatga agaacccaat
     4261 caaatatatc cgccaaccca aacctaagcc tactgtgccc attaggatta taattgtggg
     4321 gcctccaaaa tctgggaaaa ctacagttgc caaaaaaatt acaagtgaat atgggttaaa
     4381 gcatttatca ataggaggag ctttgcgtta tgtactaaac aatcacccgg aaacagagct
     4441 ggcacttatg ttaaattggc atcttcataa aggaatgaca gcacctgatg aactggctat
     4501 tcaagcctta gaactttctc tgatggaaag tgtgtgcaat actgcaggtg ttgtcatcga
     4561 tggatatcct gtaactaaac atcaaatgaa tctcttggaa gctaggtcaa tcattcccat
     4621 ggtcatcttt gaattgagtg tgccttccaa ggagattttc aaaagattgc tcctagaaaa
     4681 agaaaatgaa caaagattgc cttatccatt gcacaatagt gcacaaattg tagctgtcaa
     4741 taatgtaaag tatcgcaaaa atattggtga gattaggcaa tattatcaag aacagcatca
     4801 gaactggtat gtgattgatg gatttcacag caaatggtgg gtatggaatg aagtcattaa
     4861 gaatgttcaa atggtgaata aatacatgca gacatacctg gaaagaataa aagcaggaaa
     4921 agctgcctgc attgacaagt tatgtatcac acctcaagag ctgctttctc gcctgggaga
     4981 atttgaacag ttctgccctg tcagcctggc agaatcccag gaattatttg attgctctgc
     5041 aactgactcc ttggaatttg cagcagagtt cagggggcac tactataaaa tgagttctca
     5101 ggaaaaactg aataaattct tggagaaccc agaattgtac gtgcctccct tagcacctca
     5161 tccactccca tctgctgaca tgatccccaa aagactgaca ctgtcagagc tgaagagtcg
     5221 attccctaag tgtgcggagc tccagggcta ctgtccagtg acctataagg atggaaacca
     5281 aagatatgaa gctctagtac ctggtagcat taactatgct ttagaatatc ataatcgtat
     5341 atatatttgt gagaacaaag aaaaactcca gaaatttttg aggtcgccac tgaaatactg
     5401 ggaacagaag cttccacaca agcttccccc attaagggaa ccgatacttc ttactagtct
     5461 tcctttgcct ggatatctgg aacagggtat tgcaacttct ctaattaaag caatgaatgc
     5521 agcgggatgc ttaaagccca agttcccctt tttaagtata aggagatctg cactgctata
     5581 tatagcactt catctcaaag catttaatcc caaaggttcc gaatacacaa gaaaaaagta
     5641 taagaagaag atggagcagt ttatggagag ttgtgaactc ataacatact tgggtgccaa
     5701 gatgaccaga aaatacaagg aacctcagtt cagagccatt gactttgatc ataagttaaa
     5761 gacctttctc tctctcagaa atatagaccc aattaatggg tagtttactt aggtgatagc
     5821 agcctgaatc tcaagagtta tctgaaagtg atagagggaa actgagagaa gtagattgaa
     5881 aatctgggcc tcttggaagt acttttgcct cctgagcaag gtaccatggc tgccagactt
     5941 caggtgaact caaaggtctg ccagccagga aggagcactc ttatggaaac aagttttaat
     6001 acaattttaa aatgtattgc tctttgcctg aactttgatg ctttaacaaa ataaacattc
     6061 tatttataat tccatataga aaagttaagt gacttattta ataaatgtat tattttcctt
     6121 tttaacattt tcagtagaaa agtcagtctc tgttaaaatt actcattaaa tgttagaaag
     6181 ctttaagaca tttaacattg ttataaatga aaccaaaata tgggttatac attttacata
     6241 caaaactgtt tgtgaacttt gtgaacataa gatactatca ttttcccaat aaaataaatg
     6301 gattttgcaa caactta
//
