LOCUS       NM_018836              12102 bp    mRNA    linear   PRI 12-AUG-2024
DEFINITION  Homo sapiens adherens junctions associated protein 1 (AJAP1),
            transcript variant 1, mRNA.
ACCESSION   NM_018836
VERSION     NM_018836.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12102)
  AUTHORS   Fruh,S., Boudkkazi,S., Koppensteiner,P., Sereikaite,V., Chen,L.Y.,
            Fernandez-Fernandez,D., Rem,P.D., Ulrich,D., Schwenk,J., Chen,Z.,
            Le Monnier,E., Fritzius,T., Innocenti,S.M., Besseyrias,V.,
            Trovo,L., Stawarski,M., Argilli,E., Sherr,E.H., van Bon,B.,
            Kamsteeg,E.J., Iascone,M., Pilotta,A., Cutri,M.R., Azamian,M.S.,
            Hernandez-Garcia,A., Lalani,S.R., Rosenfeld,J.A., Zhao,X.,
            Vogel,T.P., Ona,H., Scott,D.A., Scheiffele,P., Stromgaard,K.,
            Tafti,M., Gassmann,M., Fakler,B., Shigemoto,R. and Bettler,B.
  TITLE     Monoallelic de novo AJAP1 loss-of-function variants disrupt
            trans-synaptic control of neurotransmitter release
  JOURNAL   Sci Adv 10 (28), eadk5462 (2024)
   PUBMED   38985877
  REMARK    GeneRIF: Monoallelic de novo AJAP1 loss-of-function variants
            disrupt trans-synaptic control of neurotransmitter release.
REFERENCE   2  (bases 1 to 12102)
  AUTHORS   Yang,L., Liu,J., Li,S., Liu,X., Zheng,F., Xu,S., Fu,B. and Xiong,J.
  TITLE     Based on disulfidptosis, revealing the prognostic and immunological
            characteristics of renal cell carcinoma with tumor thrombus of vena
            cava and identifying potential therapeutic target AJAP1
  JOURNAL   J Cancer Res Clin Oncol 149 (12), 9787-9804 (2023)
   PUBMED   37247081
  REMARK    GeneRIF: Based on disulfidptosis, revealing the prognostic and
            immunological characteristics of renal cell carcinoma with tumor
            thrombus of vena cava and identifying potential therapeutic target
            AJAP1.
REFERENCE   3  (bases 1 to 12102)
  AUTHORS   Li,R., Chen,Y., Wu,J., Cui,X., Zheng,S., Yan,H., Wu,Y. and Wang,F.
  TITLE     LncRNA FGF14-AS2 represses growth of prostate carcinoma cells via
            modulating miR-96-5p/AJAP1 axis
  JOURNAL   J Clin Lab Anal 35 (11), e24012 (2021)
   PUBMED   34655124
  REMARK    GeneRIF: LncRNA FGF14-AS2 represses growth of prostate carcinoma
            cells via modulating miR-96-5p/AJAP1 axis.
REFERENCE   4  (bases 1 to 12102)
  AUTHORS   Xu,C., Liu,F., Xiang,G., Cao,L., Wang,S., Liu,J., Meng,Q., Xu,D.,
            Lv,S., Jiao,J. and Niu,Y.
  TITLE     beta-Catenin nuclear localization positively feeds back on
            EGF/EGFR-attenuated AJAP1 expression in breast cancer
  JOURNAL   J Exp Clin Cancer Res 38 (1), 238 (2019)
   PUBMED   31171012
  REMARK    GeneRIF: This study demonstrated that AJAP1 acted as a putative
            tumor suppressor while beta-catenin nuclear localization positively
            fed back on EGF/EGFR-attenuated AJAP1 expression in breast cancer,
            which might be beneficial to develop new therapeutic targets for
            decreasing nuclear beta-catenin-mediated malignancy in breast
            cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 12102)
  AUTHORS   Trynka,G., Zhernakova,A., Romanos,J., Franke,L., Hunt,K.A.,
            Turner,G., Bruinenberg,M., Heap,G.A., Platteel,M., Ryan,A.W., de
            Kovel,C., Holmes,G.K., Howdle,P.D., Walters,J.R., Sanders,D.S.,
            Mulder,C.J., Mearin,M.L., Verbeek,W.H., Trimble,V., Stevens,F.M.,
            Kelleher,D., Barisani,D., Bardella,M.T., McManus,R., van Heel,D.A.
            and Wijmenga,C.
  TITLE     Coeliac disease-associated risk variants in TNFAIP3 and REL
            implicate altered NF-kappaB signalling
  JOURNAL   Gut 58 (8), 1078-1083 (2009)
   PUBMED   19240061
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   6  (bases 1 to 12102)
  AUTHORS   Resch,E., Quaiser,S., Quaiser,T., Schneider,G.,
            Starzinski-Powitz,A. and Schreiner,A.
  TITLE     Synergism of shrew-1's signal peptide and transmembrane segment
            required for plasma membrane localization
  JOURNAL   Traffic 9 (8), 1344-1353 (2008)
   PUBMED   18485053
  REMARK    GeneRIF: synergism between a signal peptide and a transmembrane
            segment to mediate plasma membrane localization is essential for
            structural and/or functional integrity of shrew-1
REFERENCE   7  (bases 1 to 12102)
  AUTHORS   Schreiner,A., Ruonala,M., Jakob,V., Suthaus,J., Boles,E.,
            Wouters,F. and Starzinski-Powitz,A.
  TITLE     Junction protein shrew-1 influences cell invasion and interacts
            with invasion-promoting protein CD147
  JOURNAL   Mol Biol Cell 18 (4), 1272-1281 (2007)
   PUBMED   17267690
  REMARK    GeneRIF: shrew-1 has a function in the regulation of cellular
            invasion, which may involve its interaction with CD147
REFERENCE   8  (bases 1 to 12102)
  AUTHORS   Jakob,V., Schreiner,A., Tikkanen,R. and Starzinski-Powitz,A.
  TITLE     Targeting of transmembrane protein shrew-1 to adherens junctions is
            controlled by cytoplasmic sorting motifs
  JOURNAL   Mol Biol Cell 17 (8), 3397-3408 (2006)
   PUBMED   16707570
  REMARK    GeneRIF: Adaptor protein complex AP-1B is involved in retaining
            shrew-1 at the basolateral membrane by postendocytic sorting
            mechanisms.
REFERENCE   9  (bases 1 to 12102)
  AUTHORS   McDonald,J.M., Dunlap,S., Cogdell,D., Dunmire,V., Wei,Q.,
            Starzinski-Powitz,A., Sawaya,R., Bruner,J., Fuller,G.N., Aldape,K.
            and Zhang,W.
  TITLE     The SHREW1 gene, frequently deleted in oligodendrogliomas,
            functions to inhibit cell adhesion and migration
  JOURNAL   Cancer Biol Ther 5 (3), 300-304 (2006)
   PUBMED   16410724
  REMARK    GeneRIF: While a mutation was not detected in the coding region of
            the SHREW1 gene in oligodendrogliomas, SHREW1 expression
            restoration resulted in suppression of cell adhesion and migration,
            thus, SHREW1 inactivation may lead to oligodendroglial
            tumorigenesis.
REFERENCE   10 (bases 1 to 12102)
  AUTHORS   Bharti,S., Handrow-Metzmacher,H., Zickenheiner,S., Zeitvogel,A.,
            Baumann,R. and Starzinski-Powitz,A.
  TITLE     Novel membrane protein shrew-1 targets to cadherin-mediated
            junctions in polarized epithelial cells
  JOURNAL   Mol Biol Cell 15 (1), 397-406 (2004)
   PUBMED   14595118
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL391808.9, AY282806.1, AF175409.1 and AL023586.1.
            
            On May 17, 2019 this sequence version replaced NM_018836.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Both variants 1 and 2 encode the same protein.
            
            CCDS Note: This CCDS ID represents the protein described in PMIDs:
            14595118 and 17267690. This protein is encoded by two different
            splice variants that are supported by AF175409.1 and AL049673.1. It
            should be noted that this transcript is predicted to undergo
            nonsense-mediated mRNA decay (NMD). However, the protein is
            represented because it was detected endogenously in PMID: 14595118.
            It is likely that the majority of transcripts representing this
            variant will undergo NMD, while some low level of NMD escape may
            allow for the expression of this protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF175409.1, AY282806.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1970526, SAMEA2145245
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000378191.5/ ENSP00000367433.3
            NMD candidate          :: PMID: 14595118
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-436               AL391808.9         6516-6951
            437-939             AY282806.1         1-503
            940-2757            AF175409.1         816-2633
            2758-3359           AL023586.1         49554-50155
            3360-12102          AL023586.1         50156-58898
FEATURES             Location/Qualifiers
     source          1..12102
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.32"
     gene            1..12102
                     /gene="AJAP1"
                     /gene_synonym="MOT8; SHREW-1; SHREW1"
                     /note="adherens junctions associated protein 1"
                     /db_xref="GeneID:55966"
                     /db_xref="HGNC:HGNC:30801"
                     /db_xref="MIM:610972"
     exon            1..846
                     /gene="AJAP1"
                     /gene_synonym="MOT8; SHREW-1; SHREW1"
                     /inference="alignment:Splign:2.1.0"
     CDS             818..2053
                     /gene="AJAP1"
                     /gene_synonym="MOT8; SHREW-1; SHREW1"
                     /note="adherens junction-associated protein 1;
                     transmembrane protein SHREW1; membrane protein shrew-1"
                     /codon_start=1
                     /product="adherens junction-associated protein 1
                     precursor"
                     /protein_id="NP_061324.1"
                     /db_xref="CCDS:CCDS54.1"
                     /db_xref="GeneID:55966"
                     /db_xref="HGNC:HGNC:30801"
                     /db_xref="MIM:610972"
                     /translation="MWIQQLLGLSSMSIRWPGRPLGSHAWILIAMFQLAVDLPACEAL
                     GPGPEFWLLPRSPPRPPRLWSFRSGQPARVPAPVWSPRPPRVERIHGQMQMPRARRAH
                     RPRDQAAALVPKAGLAKPPAAAKSSPSLASSSSSSSSAVAGGAPEQQALLRRGKRHLQ
                     GDGLSSFDSRGSRPTTETEFIAWGPTGDEEALESNTFPGVYGPTTVSILQTRKTTVAA
                     TTTTTTTATPMTLQTKGFTESLDPRRRIPGGVSTTEPSTSPSNNGEVTQPPRILGEAS
                     GLAVHQIITITVSLIMVIAALITTLVLKNCCAQSGNTRRNSHQRKTNQQEESCQNLTD
                     FPSARVPSSLDIFTAYNETLQCSHECVRASVPVYTDETLHSTTGEYKSTFNGNRPSSS
                     DRHLIPVAFVSEKWFEISC"
     exon            847..1646
                     /gene="AJAP1"
                     /gene_synonym="MOT8; SHREW-1; SHREW1"
                     /inference="alignment:Splign:2.1.0"
     exon            1647..1734
                     /gene="AJAP1"
                     /gene_synonym="MOT8; SHREW-1; SHREW1"
                     /inference="alignment:Splign:2.1.0"
     exon            1735..1980
                     /gene="AJAP1"
                     /gene_synonym="MOT8; SHREW-1; SHREW1"
                     /inference="alignment:Splign:2.1.0"
     exon            1981..2112
                     /gene="AJAP1"
                     /gene_synonym="MOT8; SHREW-1; SHREW1"
                     /inference="alignment:Splign:2.1.0"
     exon            2113..12102
                     /gene="AJAP1"
                     /gene_synonym="MOT8; SHREW-1; SHREW1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3314..3319
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AJAP1"
                     /gene_synonym="MOT8; SHREW-1; SHREW1"
                     /note="hexamer: ATTAAA"
     regulatory      3340..3345
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AJAP1"
                     /gene_synonym="MOT8; SHREW-1; SHREW1"
                     /note="hexamer: ATTAAA"
     polyA_site      3340
                     /gene="AJAP1"
                     /gene_synonym="MOT8; SHREW-1; SHREW1"
                     /note="major polyA site"
     polyA_site      3359
                     /gene="AJAP1"
                     /gene_synonym="MOT8; SHREW-1; SHREW1"
                     /note="major polyA site"
     regulatory      11660..11665
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AJAP1"
                     /gene_synonym="MOT8; SHREW-1; SHREW1"
                     /note="hexamer: AATAAA"
     polyA_site      11691
                     /gene="AJAP1"
                     /gene_synonym="MOT8; SHREW-1; SHREW1"
     regulatory      12013..12018
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AJAP1"
                     /gene_synonym="MOT8; SHREW-1; SHREW1"
                     /note="hexamer: AATGAA"
     polyA_site      12032
                     /gene="AJAP1"
                     /gene_synonym="MOT8; SHREW-1; SHREW1"
ORIGIN      
        1 agacgcgagg agggaggcgg ctgagcagcg cgggcggctc tgcggcgggc gcggtgggcg
       61 cgggcggcgg ggccccggga tccccgcgcg cctcctccgc gcggcgccgc cgccgcgcgt
      121 ccccacgccc cgcgctccac ggcgccctcg ccccgcgcgc ctctcgtgcc cgcctcctgc
      181 cagtctccgg gccgcggccg tctgcagagc gagcgctcag acggagcccc cgggcaactt
      241 gagtggcgcc gatcggcggc ggagcctctg gcagagcccc ccgacccggc agcgcggagg
      301 ggactcgcgt ccgtccgcgt cgcgtcaccc caaaccctaa gcagcgccgc cccatcggcg
      361 cggagctccg gctggaggca agagccgcgc gccgggagac acgcaccgtg agcggcagcg
      421 ccgccggcct ccctcctcgc cgccccggag ggcgaagccg cggctcccca gccctttgct
      481 gcgccgcgac agcgccggaa cacgccccgc ctcgctgccg ccttcgctgt gccgccggcg
      541 gagggggccg cgagccccgc gccccggccg gaggatgtgc gcccccgcgg gccgcccagc
      601 ctgagccatg cgccccaacg gcggcggcgc gccggccggc atggagcccc gcgcggccgc
      661 gctctgactc gctgtgcgcc ccgcggccgg cgggcggcgg gaggcggcgg accgagagcc
      721 ggagaccggc gccgcgggac ggaagcgagc gggcgcgggc gccgcgcaga tggcctgggc
      781 gagccaggtc tgaggccccg ctccccgaaa cgtgaccatg tggattcaac agcttttagg
      841 actcagctcc atgtccatcc gctggccggg ccgccccctc ggaagccatg cctggatact
      901 gatagccatg tttcagctcg ccgtggacct gcccgcctgt gaggccctgg gcccggggcc
      961 ggagttctgg ctcctgccgc ggtcgccgcc ccggccgccc cggctgtgga gttttaggag
     1021 tggacagcca gcgcgggtcc cggccccggt gtggagcccc cggccgcccc gagtggagcg
     1081 gatccacggg cagatgcaga tgcctcgagc cagacgggcc cacaggcccc gggaccaggc
     1141 ggccgccctc gtgcccaagg caggactggc caagccccca gctgctgcca aatccagccc
     1201 ttccctcgcc tcttcgtcct cgtcctcgtc ctccgcggtg gccggtgggg ccccggagca
     1261 gcaggccctc ctgaggaggg gcaagaggca cctgcagggg gacggtctca gcagcttcga
     1321 ctccagaggc agccggccca ccacagagac tgagttcatc gcctgggggc ccacggggga
     1381 cgaggaggcc ctggagtcca acacatttcc gggcgtttac ggccccacca cggtctccat
     1441 cctacaaaca cggaagacaa ctgtggccgc caccaccacc accaccacca cggccacccc
     1501 catgacgctg cagactaagg ggttcaccga gtccttggat ccccggagaa ggatcccagg
     1561 tggggttagc acaacggagc cttccaccag tcccagcaac aacggggaag tcacccagcc
     1621 cccaaggatt ctgggggagg cctcaggtct ggctgtccat cagatcatca ccatcaccgt
     1681 ctccctcatc atggtcatag ctgctctcat cacaactctt gtcttaaaaa attgctgtgc
     1741 ccaaagcggg aacactcgtc ggaacagcca ccagcggaag accaaccagc aggaggagag
     1801 ctgccagaac ctcacggact tcccctcggc ccgggtgccc agcagcctgg acatattcac
     1861 ggcctataac gagaccctgc agtgttctca cgagtgcgtc agggcatctg tgcccgtgta
     1921 caccgatgag acgctgcact cgacgacggg ggagtacaaa tccacattta atggaaaccg
     1981 accctcctct tctgatcggc atcttattcc tgtggccttc gtgtctgaga aatggtttga
     2041 aatctcctgc tgactggccg aagtcttttt tacctcctgg gggcagggca gacgccgtgt
     2101 gtctgtttca cggattccgt tggtgaacct gtaaaaacaa aacaaacaaa acaaaacaaa
     2161 aaagacaaaa cctaaaactg agctatctaa gggggagggt ccccgcacct accacttctg
     2221 tttgccggtg ggaaactcac agagcaggac gctctaggcc aaatctattt ttgtaaaaat
     2281 gctcatgcct atgggtgact gccttctccc agagttttct ttggagaaca gaaagaagaa
     2341 aggaaagaaa ggaaccagag gcagagagac gaggataccc agcgaaaggg acgggaggaa
     2401 gcatccgaaa cctaggattc gtcctacgat tctgaacctg tgccaataat accattatgt
     2461 gccatgtact gacccgaaag gctcggccgc agagccgggg cccagcgaat cacgcagaga
     2521 aatcttacag aaaacagggg tgggaatctc ttccgataga gtcgctattt ctggttaata
     2581 tacatatata aatatataaa tacaaacaca cacacacact ttttttgtac tgtagcaatt
     2641 tttgaagatc ttaaatgttc ctttttaaaa aaaagaattg tgttataggt tacaaaatct
     2701 gatttattta acatgcttag tatgagcaga ataaaccagt gttttctact ttggcaactc
     2761 acgtcacaca catattacac acatgtgcgc attacacaca cacaatacac atacatgcat
     2821 atagacgcat ctattggaaa tgcagttcca caggtgagca tgttctttct ggtgacctgg
     2881 tattccatca ccattcaccc caggggacag cctcgaccga gacaaggagg cccttaaatg
     2941 acagcctgca tttgctagac ggttggtgag tggcatcaaa tgtgtgactt actatcttgg
     3001 gccagaacta agaatgccaa ggttttatat atgtgtgtgt atatatatat atatatatat
     3061 atatatatat atatatatgt ttgtgtgtgt atatatatat atatatatat atgtttgtgt
     3121 gtgtatatat atgtttgtgt atatatatac acatatgcat acatatgatt tttttttttc
     3181 atttaagtgt tggaagatgc tacctaacag ccacgttcac atttacgtag ctggttgctt
     3241 acaaacgggc ctgagcccct ggttgggtgg gtggtggatt cttggacgtg tgtgtcatac
     3301 aagcatagac tggattaaag aagttttcca gttccaaaaa ttaaaggaat atatccttat
     3361 gatgtgtgtg tgtaatatca gggcagaact tagacatacg tgaagggccc cggttggttt
     3421 gaaaacgaaa aatagtcatt ctgtgtgcaa accacaaggc tgccccagtc aggcagcgcc
     3481 ctgacctggc ctgtgctgca ttgccttccc ttgcgcaggt gggcaggtgt ggcccgcttt
     3541 ttctagggcc caagggtgac ttggaatctt gggggaagcc tcggcaaaga tgccgttctc
     3601 ccgtcagctt ccattggccg atggatgttt ctgttttgtc accgagaatc ctactataaa
     3661 ctacccagtg gaaacagggg ccccagacct gagcctcagt acagagaatc ccaaccacac
     3721 acactttatt aaaaatactg ccatgccgcc gggcctccct cacaagcctc acctgaaacg
     3781 ggctcactgg tgccccagac ttgctgtcaa cagcgggtgc tgtagggatc tagggatgca
     3841 tctcatggcc cacgctgtgc ctccttaaag gatgtttcct gtccatggga gacgcctaac
     3901 atggtgtggg tccctgtctc caaagactat atttctcacg cgtggcccac gtgcagcttt
     3961 aatttcagca gtagagtgca tgagccaatt cagtgaaatg tcacaggcca ggcaaaaagg
     4021 ttaaagatca gacagacagc tgaccttact gccctcaatg gccaggctga tgacctgatt
     4081 cttcccttgg gctcagtctc cctctaaaac accttgtgat atgcttcaga agttcacttt
     4141 tgtatctcct gaaacatgtg aacctagagt aaggcctccg gaggtgaaag gattgttttt
     4201 gtgatgcagc agtgttgaga tctgtatcca tggtattgct cacaaattag gaaaagttgt
     4261 tctttagttt ttgttctgtc atttattttc cccccaagct ttggcacatc ctgagacaat
     4321 gcaaacatcc gccactggca tttggccgtc tccaggttcc tggcactcac ctgcatggcg
     4381 ctccagcctg cagctctctc tgcgcgccat ccggcctcgt ccatcccatt ctccttcaat
     4441 cactccctcc cgctgaggtg ggcctcctga ctccaggtga gcacagcgtg gtgcgagtgc
     4501 atggtgaggg ctggcggcgg ccaacaggca gtggggtggc tgctctggtc tctgacattc
     4561 tgagctccag agtgagccca actaaggagg aggtgacggc cggggggccc tgttggttcg
     4621 ctgatcatga gaacctagga ttcccgaggg actcctggta tctgctctgt caactcccag
     4681 ctgctcagcc cccttctctg accatgtgcg gcaccctgga tgccctgaac tctttcctgg
     4741 gtccataaga catcgagcct tggaaggaag cgtaatgcaa caaccctctt gtccccgata
     4801 agagccacag attcaaggca gggctgtgag acccacacag gcacaaacac cagacaagca
     4861 agtgcatgcc cattccacac ccaaaagtct ggttctgtac caacatgaca tccgtcaagg
     4921 taaatccaca caaaggctgc tatttctgga tgattgaggc agtgtctgta gacgtgttac
     4981 tttacttttt taaatggtta cctgctctcc cagacccctc acctgggatt gtcgtctagg
     5041 taagaggaga taatgagtga aagtgactgg gcctcggaaa tgcagcccca cccacggtga
     5101 agtctgcacc agagcacaga cttgtcctac ttcgggccat atgtataggt aatgcccatg
     5161 tactttgtgt gtcggtggct gctgatttgg cctgatcagt ccttctgcag ccttggggca
     5221 gtgtttcaag ctagttggtg tcctctttcc tctgcttgac ccctggaatt cagagtctaa
     5281 tagtagacac ctcccatccc ccacccaagg cctggtcctt tcctttgctt gagcttttat
     5341 tcctcaagaa aagaactcca aggaagggtg atgtggtttg ttagggttcc ccagttttcg
     5401 tccacttcca tcttattcct cggagatccc gcaattgcaa accccctggc agcctcctgg
     5461 cacctgcatt tgcctttaaa gcttagaaac agctgggaat gcactccttg tacagtccag
     5521 tccgttttca gacaaagtcg ctgctagtct tcacacgctt gatgtcactt tgtctgattg
     5581 gaatatgcgt gtcattgctc ttgtttccga gtttggcgtg agaaaggcag ggttctccag
     5641 gcatggtcag ggagcttccc caccgtttcc cattgggctc agattccagc cattaccgag
     5701 acaaatccaa gccttctcac gcaacgtgga agcgtggtga caccctgggg tgtgtggagg
     5761 tggctgctag tgttcctatt tactgccctc cttgcacaca gaagggagag gccacttagg
     5821 ggaccttgtc ttctccgtag tatttctagt ccttgccaat cttgaacttg agtggacttc
     5881 tcatattaca ttcagctccc agaaattcac tccagtcctt cctggagcat tgtgaaatta
     5941 gaaacctgaa ctcatcagca aaccccaccc actctttgaa tcgggacacc aggaaacccg
     6001 ttactactaa tgtgatctta ccatcttaat cctttctttc aaaaatacgt gactctttca
     6061 cagccatatt ttagcgtcag gaaaagccat gcttagaggg attggtcttc aaaattttgt
     6121 gtgtgatgcc caatgtgtag gccatattga aaggtgaacg caccaaagga tcgaccctgt
     6181 caccttggat ggtctctatt ttgaccctgt tgcccatgga gaggtatttt acccttttgc
     6241 ttctttgctc aggtggtcca gggttgtctg tagcccagga agcggactga ccctgtatct
     6301 tgaaagataa gaccaggaac cgggagtgag gctgggactc tcactcagcc ttttcacggt
     6361 cagtcttggt cttctcatac ttatttcaag atggcaccag gacacacctt cttttgtcca
     6421 cctgttgaca ccatcaccct ctttcatcaa ggtgatctct ttcatcaggg catatatgag
     6481 acttggtgct ccaccagact gtcttctccc ctacccctgc cccttccaca aagccatttc
     6541 tcaagtcgca gtgggaaaag ataccaagac acctgatttc ttgaaccatt cttgcttcag
     6601 catgtgaatt ctttaagaca ctactcacat atgccattgg agagaaacaa cccgttctgc
     6661 atcaccataa tgaaccgatc agtcattcat cctcttattc aacaaatact tgttccactt
     6721 ctaccacact ggccatacag ttggaggcaa gatgggcaca gttatggcct tgtgggcttt
     6781 atattctaca gtgggaggag gtggatggtt aatgtgaaag caaaccaaaa taatttccag
     6841 agagagaaga ggcaggaaga gagcagagca gagtgatggg gctgggggca gagaaggaga
     6901 gaggggaaga gggagagagt ggggggcatt gaaggggaag aaagagaggc aggggttgtc
     6961 tacgtctcct cctcctgcga cccatcacct actggaaaat taccacttgc tgcttgaggg
     7021 ttggtctggt gttggatgga ggaagaaggc cttagtctta gctcttggat aaaatgcagt
     7081 gcagcactga agataagaca tcgcagttgt tacgacgcct ggttctccac caaattcctc
     7141 tgtcattcca gcaagagtgg aagcagaggg gcaggggcag gggcaggatg agggctgctg
     7201 ccctggtgat gagcttgccc catccctggg cactggctct gcccagcccc tcccatgttg
     7261 gtcccatctg tcaggttgcc aggccaagca ggtctcaggt cagccaggtc tcaaggagga
     7321 taagggggtt caacgtcagc gacttggccc acggggcacg ggcaccttgg ggatgccatt
     7381 cttcttggtg ccagaaggca gctgcatctc cagaagctcc cccagcactg agctcactta
     7441 gtggcatcct tcttaaacag catctgcttt taagtaagca gctattccaa aacatgccgt
     7501 gattcatgta atttttgagt ggttactttg gtgcagtttg tcaatttgct ctaccatact
     7561 gttttttgat tatttgtttg ttttctagtg taaatagcac agcccacata aatgagcgaa
     7621 actcatcatc tgaggatctt tgattattct tccccactta ttgtacattg atgtgccgcc
     7681 tgctaggaag ataaactccc tctttgctca ctctgagcca ggatctctgt gttagttaca
     7741 ctcagcctta tctgagcatt ccagggaggt cttaaatgaa tgtttggggt tttgaaacat
     7801 gctaccagcc cttccttcca aagagcatga agcatggctc tgtgtggttt tgtgtgtcta
     7861 ggtttagagc ttgtatttgg atgtaaggag tgactaaaaa caggtctagg cagagaactg
     7921 ctatctttta aaccacccca gccctcagtc gaacagaaag aatgagcgtc ttctgatgtc
     7981 aaatcattct agaaaggtct tagctagtga gcacaaagta gccaatcccg ggagtttata
     8041 tgggcctcca tgtgaccaag ccatccctag ctaggaaact taactctttg cacaacctcc
     8101 ctttatgctg aagggccctt gggaacatgg gaacatttgc actttgtcag aggagtggcc
     8161 ccaccctact ctctgcctga aacacaaagc agctcccctc agaacagcca gcccggtgca
     8221 gaagcacttg aacttctcat cttccatctt atttcacttt ggttgcaggg agttaaatac
     8281 ataggccaca gcagactaag ggagcggtgt gcacaatggc ccccagaata tccagtgacc
     8341 caagtgatgt tagtcttagg gtccctaagg cagtagggca tggggaggaa agttctctcc
     8401 atcgattccc aaacacacca gccacaagtc cacactccac acctgtaaac gcacagccca
     8461 tgccccattg taaaggtgac tgaagagaat ggtcaactcg gtggtgatgg gcctgttttg
     8521 gagctaaaag cataaaatga atcctccctg tagctccatt gccttttcct cgtgaaggat
     8581 tccctgctaa atgttctaag agttactgta catagaatca agagagcagt tttgaatgag
     8641 ccgtaaatct gagtcatata taagaggcat aatataaatt gggccaagct tgcccatgtg
     8701 ggacacacag gtttatagag attaaacact agtgagtagc gggggaaggg aacagtgact
     8761 gtataaatgg ccatataaat gacctacttg tcttctcatg taggctggaa tcagaaccta
     8821 cgtatgctgt agataacagt caattaaatg ggttaatgtt ctaagccaag agagttctaa
     8881 gccaagacat gtgcttggga ctggattaac tcttgtcaat atcttggatt ccatagtaaa
     8941 acatctgtta agtaacatgc ggcaggaaag gatgcaggga gcattggact gtaggaaatt
     9001 ctcctttata caagttaatt agttttgtct ctggtggctg tctcttaaaa cagaatcccc
     9061 tggagacccg ggttttcatt ttgtaattca acacccaaat atttgcaaca ctaaataaca
     9121 actccctaaa gacttcagtc ttgcatgggc ataagcaact cacactggtt agcttttgtc
     9181 agatttcgtc gtttccgcag caaaatttct tagtgttgat gttgatagct tctgggttgg
     9241 caaactggca gttcactcta gtcttactgg gttgggcaag tccttgcccc tagaaaattg
     9301 gggatagctt tggccatggg aggagcaaga acactacgca gtcgtaagct ctgttttttg
     9361 ttttgttttg ttttgttttg ttttcggcac cattatgtac gatgaagact tacagccact
     9421 gctaatacag cataggggac actttgagga gccctagtat cattgtttga aatcattgta
     9481 cagggaactg caggggtgaa caccattgta ccaatccccg ggccagtgtc tcatttccta
     9541 acagaatcat cccattcttg ttcactcttc agtcatccca agccaaaagg gaaaattcga
     9601 aacccactgt gacaccccaa ccctaatttc caacctttgg aggactcacc tggggcagaa
     9661 gggagaaagg gctttgctga agtctcaggc tgccgagagc catgtatggg cataggagca
     9721 agctgatggc atggctcttg acaaggtttt ctatagggca gcagctgctg ctcccagggg
     9781 aaccctctcc tgttgcctcc gcagtctatt ctggggtgga aacaccattc tctattattt
     9841 ctctcacata catgtggatt tctaatatca agttatgtga ctgcctacca cttaaggcct
     9901 acaaaaccac atgggagttt ctttattgcg tatgcagcca gtcaacatga atgggaccct
     9961 agaaactgtg aggagcagga ttctctaagg tgctcttaga tacggtgtca cttccacata
    10021 caagcacaat tctcccagtt aaggggctgg aagacaagcg gggcaactgg ttttgaaaag
    10081 cccgctggcg tgccagaggg ggtgtttgcc cacaccctcc ttgaatttaa ctgccacaat
    10141 ctatccgcag atgtgttttg ttctatttct tgtttttcac tagcgtttgc gttgcttcct
    10201 ctgaagccag agggtgaaag gccctagcaa agttagttat cagtcaactg atgataactg
    10261 tgatccttaa agatgaattc ccagcctgag gtgacacaca gaggttcagc agacgtctca
    10321 ggatctgtca tatgtcatgt tgcttggtgt gaagatggaa gaacaaagtc cacatcagtt
    10381 tctgctcctt caaacagtgt gtcgatatga aacattgaga tttggcagaa acatgtgcct
    10441 agtttgcagc accaaatact ggtcttctca gaagtgtttc tgtttttttg tttttgtttt
    10501 tgtttttgtt tttgtttttg ttttgtttaa tttcctcttc tggcatccca atttggatca
    10561 tttcctctct ttctttcctg gagaaacagg atggcaggtg ctggagccca gagagggggt
    10621 caccctccag ctaaaatgat ttaaatggaa aggagaatga cagcaaagcc tcacgtgaac
    10681 tttaatgacc ttatcaatga cctttactaa ctgactcttg gtacccggat tgttgagagg
    10741 tgagtgggac taaaagccag gatcaagatc aggttgctcc acacatttgc agatcaggaa
    10801 ccccgggaat atgagttaga cccagaaagt attgaactgg acattgaatg aagcttgagg
    10861 ccatgcattg ataacatttt tagagtctgt tacattattt tctcaaaagg aacacatgtt
    10921 tactacaaac catttagaaa ataccaataa ccaaacagaa gggaaatgtc tggaaccttc
    10981 cagttgttta attctgtaga aactgttcac gaaaggctga atgagtgggg acaagtcaca
    11041 gagatctcgc catggttagc accagggaca agtctggctc taatgtatgt tttatgaagt
    11101 ctgattagct ttgaaatgca tctttgccag ctgaacttgt catgcactaa acttcccatt
    11161 gatcattaga gtgtctacga ttaagtggaa aaggtggtca accatttgga tgtttgactt
    11221 tcacaacatc aagcattggc cattgggcgt atctgggcat tggtatggaa taaacaggat
    11281 actcagtgaa acatccaaac tattgactgg atttttccca tggggcatct gtctaagagt
    11341 cacagatctg agaaatagaa ggaccaggtc cgtgtggatg ctcctagtgc gttcaaataa
    11401 cccttgaatt caccaggtct cattgcattt atttaaagat ggagcccatt gtcccccctc
    11461 ctcctcagct cacccatgga actagaaatt cacaaacctg aagctgaacc acaggacaag
    11521 aaattaaaca gcaacggcca catggcctct tttttcacag gttttgcatt gaatgtttta
    11581 gaactttatt cccccgcccc ctcacccatg tataccaaaa ctggttttat tttcattttc
    11641 ctaaattatt ttctgataca ataaactgtt aactgtttat ttatactttt acaaggagca
    11701 cttgttattt ttcaaaagga aaacaaaagc ctctctgttt tcatgttctc cgtatatcta
    11761 taccacagct gtctctatag acgtatttat atatcatctt cttgagagag ttaacctcct
    11821 gtagtttaat gttgtgtttc ttttctttaa atgcatatca taatcatgtt aataaacaaa
    11881 aatgacacaa tatatgtggg tggggggcgg ggggcaggtg gctgggtgtg atttccgtac
    11941 aggtgaactt ttattccaga tttttttaag ttgtttctgt atttcaaagc tatgtatttg
    12001 gaaaacattg taaatgaaac acctatatta actttcctgt gtatatttag tactctgatg
    12061 ttgctattaa aaaaaaaaac ataatatgaa aaaaaaaaaa aa
//
