LOCUS       NM_001134831            6063 bp    mRNA    linear   PRI 09-OCT-2024
DEFINITION  Homo sapiens Abelson helper integration site 1 (AHI1), transcript
            variant 1, mRNA.
ACCESSION   NM_001134831 XM_005267038
VERSION     NM_001134831.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Sakrajda,K., Bilska,K., Czerski,P.M., Narozna,B.,
            Dmitrzak-Weglarz,M., Heilmann-Heimbach,S., Brockschmidt,F.F.,
            Herms,S., Nothen,M.M., Cichon,S., Wieckowska,B., Rybakowski,J.K.,
            Pawlak,J. and Szczepankiewicz,A.
  TITLE     Abelson Helper Integration Site 1 haplotypes and peripheral blood
            expression associates with lithium response and immunomodulation in
            bipolar patients
  JOURNAL   Psychopharmacology (Berl) 241 (4), 727-738 (2024)
   PUBMED   38036661
  REMARK    GeneRIF: Abelson Helper Integration Site 1 haplotypes and
            peripheral blood expression associates with lithium response and
            immunomodulation in bipolar patients.
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Karamzade,A., Babaei,M., Saberi,M., Golchin,N., Khalil Nejad Sani
            Banaei,A., Eshaghkhani,Y., Golchehre,Z. and Keramatipour,M.
  TITLE     Identification of a novel truncating variant in AHI1 gene and a
            brief review on mutations spectrum
  JOURNAL   Mol Biol Rep 48 (6), 5339-5345 (2021)
   PUBMED   34191236
  REMARK    GeneRIF: Identification of a novel truncating variant in AHI1 gene
            and a brief review on mutations spectrum.
            Review article
REFERENCE   3  (bases 1 to 6063)
  AUTHORS   Bai,Z., Hu,S., Liu,N., Wu,Q. and Kong,X.
  TITLE     [Genetic testing and prenatal diagnosis for two families affected
            with Joubert syndrome]
  JOURNAL   Zhonghua Yi Xue Yi Chuan Xue Za Zhi 37 (5), 509-513 (2020)
   PUBMED   32335874
  REMARK    GeneRIF: The proband of family 1 with Joubert syndrome was found to
            harbor homozygous c.2072delT (p.F691S*fs19) frameshift variant of
            the AHI1 gene, which may cause premature termination of translation
            of the Abelson helper integration site 1 after the 691st amino acid
REFERENCE   4  (bases 1 to 6063)
  AUTHORS   Sheu,J.J., Yang,L.Y., Sanotra,M.R., Wang,S.T., Lu,H.T., Kam,R.S.Y.,
            Hsu,I.U., Kao,S.H., Lee,C.K., Shieh,J.C. and Lin,Y.F.
  TITLE     Reduction of AHI1 in the serum of Taiwanese with probable
            Alzheimer's disease
  JOURNAL   Clin Biochem 76, 24-30 (2020)
   PUBMED   31786207
  REMARK    GeneRIF: An early event of AHI1 reduction in the body of AD
            patients was observed. Serum AHI1 may be valuable for early
            diagnosis of Alzheimer disease
REFERENCE   5  (bases 1 to 6063)
  AUTHORS   Close,J., Game,L., Clark,B., Bergounioux,J., Gerovassili,A. and
            Thein,S.L.
  TITLE     Genome annotation of a 1.5 Mb region of human chromosome 6q23
            encompassing a quantitative trait locus for fetal hemoglobin
            expression in adults
  JOURNAL   BMC Genomics 5 (1), 33 (2004)
   PUBMED   15169551
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6063)
  AUTHORS   Jiang,X., Zhao,Y., Chan,W.Y., Vercauteren,S., Pang,E., Kennedy,S.,
            Nicolini,F., Eaves,A. and Eaves,C.
  TITLE     Deregulated expression in Ph+ human leukemias of AHI-1, a gene
            activated by insertional mutagenesis in mouse models of leukemia
  JOURNAL   Blood 103 (10), 3897-3904 (2004)
   PUBMED   14751929
  REMARK    GeneRIF: in both mice and humans, Ahi-1/AHI-1 expression is highest
            in the most primitive hematopoietic cells with specific patterns of
            down-regulation in different lineages. Cells from CML patients show
            elevated AHI-1 transcripts in all disease phases
REFERENCE   7  (bases 1 to 6063)
  AUTHORS   Lagier-Tourenne,C., Boltshauser,E., Breivik,N., Gribaa,M.,
            Betard,C., Barbot,C. and Koenig,M.
  TITLE     Homozygosity mapping of a third Joubert syndrome locus to 6q23
  JOURNAL   J Med Genet 41 (4), 273-277 (2004)
   PUBMED   15060101
REFERENCE   8  (bases 1 to 6063)
  AUTHORS   Jiang,X., Hanna,Z., Kaouass,M., Girard,L. and Jolicoeur,P.
  TITLE     Ahi-1, a novel gene encoding a modular protein with WD40-repeat and
            SH3 domains, is targeted by the Ahi-1 and Mis-2 provirus
            integrations
  JOURNAL   J Virol 76 (18), 9046-9059 (2002)
   PUBMED   12186888
REFERENCE   9  (bases 1 to 6063)
  AUTHORS   Gunay-Aygun,M., Gahl,W.A. and Heller,T.
  TITLE     Congenital Hepatic Fibrosis Overview horizontal line RETIRED
            CHAPTER, FOR HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301743
REFERENCE   10 (bases 1 to 6063)
  AUTHORS   Parisi,M. and Glass,I.
  TITLE     Joubert Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301500
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AJ459824.1, AY133243.1,
            AI300295.1 and AL023693.25.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001134831.1.
            
            Summary: This gene is apparently required for both cerebellar and
            cortical development in humans. This gene mutations cause specific
            forms of Joubert syndrome-related disorders. Joubert syndrome (JS)
            is a recessively inherited developmental brain disorder with
            several identified causative chromosomal loci. Alternatively
            spliced transcript variants encoding different isoforms have been
            identified. [provided by RefSeq, Oct 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1-3 and 5 all encode the same isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DQ090887.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265602.11/ ENSP00000265602.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-220               AJ459824.1         6-225
            221-305             AY133243.1         130-214
            306-5609            AJ459824.1         226-5529
            5610-5623           AI300295.1         2-15                c
            5624-6063           AL023693.25        170846-171285       c
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q23.3"
     gene            1..6063
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /note="Abelson helper integration site 1"
                     /db_xref="GeneID:54806"
                     /db_xref="HGNC:HGNC:21575"
                     /db_xref="MIM:608894"
     exon            1..158
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            159..220
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            221..305
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            306..369
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     CDS             360..3950
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /note="isoform a is encoded by transcript variant 1;
                     contatins SH3 and WD40 domains; abelson helper integration
                     site 1 protein homolog"
                     /codon_start=1
                     /product="jouberin isoform a"
                     /protein_id="NP_001128303.1"
                     /db_xref="CCDS:CCDS47483.1"
                     /db_xref="GeneID:54806"
                     /db_xref="HGNC:HGNC:21575"
                     /db_xref="MIM:608894"
                     /translation="MPTAESEAKVKTKVRFEELLKTHSDLMREKKKLKKKLVRSEENI
                     SPDTIRSNLHYMKETTSDDPDTIRSNLPHIKETTSDDVSAANTNNLKKSTRVTKNKLR
                     NTQLATENPNGDASVEEDKQGKPNKKVIKTVPQLTTQDLKPETPENKVDSTHQKTHTK
                     PQPGVDHQKSEKANEGREETDLEEDEELMQAYQCHVTEEMAKEIKRKIRKKLKEQLTY
                     FPSDTLFHDDKLSSEKRKKKKEVPVFSKAETSTLTISGDTVEGEQKKESSVRSVSSDS
                     HQDDEISSMEQSTEDSMQDDTKPKPKKTKKKTKAVADNNEDVDGDGVHEITSRDSPVY
                     PKCLLDDDLVLGVYIHRTDRLKSDFMISHPMVKIHVVDEHTGQYVKKDDSGRPVSSYY
                     EKENVDYILPIMTQPYDFKQLKSRLPEWEEQIVFNENFPYLLRGSDESPKVILFFEIL
                     DFLSVDEIKNNSEVQNQECGFRKIAWAFLKLLGANGNANINSKLRLQLYYPPTKPRSP
                     LSVVEAFEWWSKCPRNHYPSTLYVTVRGLKVPDCIKPSYRSMMALQEEKGKPVHCERH
                     HESSSVDTEPGLEESKEVIKWKRLPGQACRIPNKHLFSLNAGERGCFCLDFSHNGRIL
                     AAACASRDGYPIILYEIPSGRFMRELCGHLNIIYDLSWSKDDHYILTSSSDGTARIWK
                     NEINNTNTFRVLPHPSFVYTAKFHPAVRELVVTGCYDSMIRIWKVEMREDSAILVRQF
                     DVHKSFINSLCFDTEGHHMYSGDCTGVIVVWNTYVKINDLEHSVHHWTINKEIKETEF
                     KGIPISYLEIHPNGKRLLIHTKDSTLRIMDLRILVARKFVGAANYREKIHSTLTPCGT
                     FLFAGSEDGIVYVWNPETGEQVAMYSDLPFKSPIRDISYHPFENMVAFCAFGQNEPIL
                     LYIYDFHVAQQEAEMFKRYNGTFPLPGIHQSQDALCTCPKLPHQGSFQIDEFVHTESS
                     STKMQLVKQRLETVTEVIRSCAAKVNKNLSFTSPPAVSSQQSKLKQSNMLTAQEILHQ
                     FGFTQTGIISIERKPCNHQVDTAPTVVALYDYTANRSDELTIHRGDIIRVFFKDNEDW
                     WYGSIGKGQEGYFPANHVASETLYQELPPEIKERSPPLSPEEKTKIEKSPAPQKQSIN
                     KNKSQDFRLGSESMTHSEMRKEQSHEDQGHIMDTRMRKNKQAGRKVTLIE"
     exon            370..494
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            495..548
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            549..1108
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1290
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1291..1510
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1511..1703
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..1799
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..1985
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1986..2138
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2139..2271
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2272..2395
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2396..2625
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2626..2732
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2733..2851
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2852..2982
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2983..3123
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3124..3320
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3321..3347
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3348..3468
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3469..3524
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3525..3687
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3688..3785
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3786..3844
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3845..3947
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3948..6063
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4281..4286
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /note="hexamer: AATAAA"
     polyA_site      4300
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
     regulatory      5586..5591
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /note="hexamer: AATAAA"
     polyA_site      5609
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /note="major polyA site"
     regulatory      6041..6046
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
                     /note="hexamer: ATTAAA"
     polyA_site      6063
                     /gene="AHI1"
                     /gene_synonym="AHI-1; dJ71N10.1; JBTS3; ORF1"
ORIGIN      
        1 acagagccgc ggcttgcttt caggacacgg gtcgctggcg ggttaggtgg ctgcgtggcc
       61 gcctcacttc gcgcacgcgc cgctaggctg ggggagttga tttgcactgc tccgggtgtc
      121 ggctccggcg ggacgggcgg ttctgcgtac ccctctccag tgtcaacctg gggctgaatc
      181 ttcaacctgt caaagccttc ttaattatgt ctgagaaaag tactaaaata ggtgccattt
      241 taaattccca gacaaggaaa aaccaaggat tagaaattaa gtagcttgcc caaggccaca
      301 catcagatcc tgattgagtc aatgctttgc attctcagtc ctctgcataa agctgagaga
      361 tgcctacagc tgagagtgaa gcaaaagtaa aaaccaaagt tcgctttgaa gaattgctta
      421 agacccacag tgatctaatg cgtgaaaaga aaaaactgaa gaaaaaactt gtcaggtctg
      481 aagaaaacat ctcacctgac actattagaa gcaatcttca ctatatgaaa gaaactacaa
      541 gtgatgatcc cgacactatt agaagcaatc ttccccatat taaagaaact acaagtgatg
      601 atgtaagtgc tgctaacact aacaacctga agaagagcac gagagtcact aaaaacaaat
      661 tgaggaacac acagttagca actgaaaatc ctaatggtga tgctagtgta gaggaagaca
      721 aacaaggaaa gccaaataaa aaggtgataa agacggtgcc ccagttgact acacaagacc
      781 tgaaaccgga aactcctgag aataaggttg attctacaca ccagaaaaca catacaaagc
      841 cacagccagg cgttgatcat cagaaaagtg agaaggcaaa tgagggaaga gaagagactg
      901 atttagaaga ggatgaagaa ttgatgcaag catatcagtg ccatgtaact gaagaaatgg
      961 caaaggagat taagaggaaa ataagaaaga aactgaaaga acagttgact tactttccct
     1021 cagatacttt attccatgat gacaaactaa gcagtgaaaa aaggaaaaag aaaaaggaag
     1081 ttccagtctt ctctaaagct gaaacaagta cattgaccat ctctggtgac acagttgaag
     1141 gtgaacaaaa gaaagaatct tcagttagat cagtttcttc agattctcat caagatgatg
     1201 aaataagctc aatggaacaa agcacagaag acagcatgca agatgataca aaacctaaac
     1261 caaaaaaaac aaaaaagaag actaaagcag ttgcagataa taatgaagat gttgatggtg
     1321 atggtgttca tgaaataaca agccgagata gcccggttta tcccaaatgt ttgcttgatg
     1381 atgaccttgt cttgggagtt tacattcacc gaactgatag acttaagtca gattttatga
     1441 tttctcaccc aatggtaaaa attcatgtgg ttgatgagca tactggtcaa tatgtcaaga
     1501 aagatgatag tggacggcct gtttcatctt actatgaaaa agagaatgtg gattatattc
     1561 ttcctattat gacccagcca tatgatttta aacagttaaa atcaagactt ccagagtggg
     1621 aagaacaaat tgtatttaat gaaaattttc cctatttgct tcgaggctct gatgagagtc
     1681 ctaaagtcat cctgttcttt gagattcttg atttcttaag cgtggatgaa attaagaata
     1741 attctgaggt tcaaaaccaa gaatgtggct ttcggaaaat tgcctgggca tttcttaagc
     1801 ttctgggagc caatggaaat gcaaacatca actcaaaact tcgcttgcag ctatattacc
     1861 cacctactaa gcctcgatcc ccattaagtg ttgttgaggc atttgaatgg tggtcaaaat
     1921 gtccaagaaa tcattaccca tcaacactgt acgtaactgt aagaggactg aaagttccag
     1981 actgtataaa gccatcttac cgctctatga tggctcttca ggaggaaaaa ggtaaaccag
     2041 tgcattgtga acgtcaccat gagtcaagct cagtagacac agaacctgga ttagaagagt
     2101 caaaggaagt aataaagtgg aaacgactcc ctgggcaggc ttgccgtatc ccaaacaaac
     2161 acctcttctc actaaatgca ggagaacgag gatgtttttg tcttgatttc tcccacaatg
     2221 gaagaatatt agcagcagct tgtgccagcc gggatggata tccaattatt ttatatgaaa
     2281 ttccttctgg acgtttcatg agagaattgt gtggccacct caatatcatt tatgatcttt
     2341 cctggtcaaa agatgatcac tacatcctta cttcatcatc tgatggcact gccaggatat
     2401 ggaaaaatga aataaacaat acaaatactt tcagagtttt acctcatcct tcttttgttt
     2461 acacggctaa attccatcca gctgtaagag agctagtagt tacaggatgc tatgattcca
     2521 tgatacggat atggaaagtt gagatgagag aagattctgc catattggtc cgacagtttg
     2581 atgttcacaa aagttttatc aactcacttt gttttgatac tgaaggtcat catatgtatt
     2641 caggagattg tacaggggtg attgttgttt ggaataccta tgtcaagatt aatgatttgg
     2701 aacattcagt gcaccactgg actataaata aggaaattaa agaaactgag tttaagggaa
     2761 ttccaataag ttatttggag attcatccca atggaaaacg tttgttaatc cataccaaag
     2821 acagtacttt gagaattatg gatctccgga tattagtagc aaggaagttt gtaggagcag
     2881 caaattatcg ggagaagatt catagtactt tgactccatg tgggactttt ctgtttgctg
     2941 gaagtgagga tggtatagtg tatgtttgga acccagaaac aggagaacaa gtagccatgt
     3001 attctgactt gccattcaag tcacccattc gagacatttc ttatcatcca tttgaaaata
     3061 tggttgcatt ctgtgcattt gggcaaaatg agccaattct tctgtatatt tacgatttcc
     3121 atgttgccca gcaggaggct gaaatgttca aacgctacaa tggaacattt ccattacctg
     3181 gaatacacca aagtcaagat gccctatgta cctgtccaaa actaccccat caaggctctt
     3241 ttcagattga tgaatttgtc cacactgaaa gttcttcaac gaagatgcag ctagtaaaac
     3301 agaggcttga aactgtcaca gaggtgatac gttcctgtgc tgcaaaagtc aacaaaaatc
     3361 tctcatttac ttcaccacca gcagtttcct cacaacagtc taagttaaag cagtcaaaca
     3421 tgctgaccgc tcaagagatt ctacatcagt ttggtttcac tcagaccggg attatcagca
     3481 tagaaagaaa gccttgtaac catcaggtag atacagcacc aacggtagtg gctctttatg
     3541 actacacagc gaatcgatca gatgaactaa ccatccatcg cggagacatt atccgagtgt
     3601 ttttcaaaga taatgaagac tggtggtatg gcagcatagg aaagggacag gaaggttatt
     3661 ttccagctaa tcatgtggct agtgaaacac tgtatcaaga actgcctcct gagataaagg
     3721 agcgatcccc tcctttaagc cctgaggaaa aaactaaaat agaaaaatct ccagctcctc
     3781 aaaagcaatc aatcaataag aacaagtccc aggacttcag actaggctca gaatctatga
     3841 cacattctga aatgagaaaa gaacagagcc atgaggacca aggacacata atggatacac
     3901 ggatgaggaa gaacaagcaa gcaggcagaa aagtcactct aatagagtaa agaattgaag
     3961 aaaagttaag agctgccgaa atgcacagag gtgaaaatga caaaccaaat ggaatttctc
     4021 ttcagagttc agaattttca gatactaagg aggaagaaag gatccactac ttcttgttct
     4081 tatgaatgac tctagaaaaa tcagaatcaa gttgtgggtg gaaaaatcaa cgtggccttt
     4141 gagttcagtt gttataaacc attgtgacta ttgttggtca aagtattggt acttatattg
     4201 ttagtaattg catcataatt acattaccag tgttggaaaa ctaatgaaga aaacactgta
     4261 attgctactc agcaaatgtg aataaaaggt gtttgcgtta ttaggatgtc tgttaagtaa
     4321 tcatttaata ttattatatt ggtaatggtt gtatgtgtga tgctatgccc agaatatgaa
     4381 gtatctgttt ttgaaattca ctttatttaa aagataagca gctgactggg cacggtgcct
     4441 catgcctgta atcctagcac cttgggaggc tgaggcaggt ggatcaccta aggtcaggag
     4501 ttcaacaaca ccagcctgac caacatggtg aaaccccatc tctactaaaa atacaaaaat
     4561 cagccgggtc tcatggcagg cacctgtaat cccatctact gaggcaggag aattgcttga
     4621 cccaggaggc agaggttgca gtgagccaag atcacgccat tgcactccag cctgggggac
     4681 agagcaagac tctatctcca aaaaacaaaa aagataagca gctttagaat atggcgcatt
     4741 caaaacagtc tcagtaacaa agacattaaa agaaaacaat ttactttcta attaaaattt
     4801 tgtgtttctt aagatcaaat catataggta acttcataga cctaaattaa aagtgatttt
     4861 tggctggact ggcaacaatg ttcccaatgt ctttactttt taaaaaaggc ttttcatatt
     4921 taagcacata cctattttgt agacttacat tgtttaatat ttattttaat cttaatattt
     4981 ttacattatt atattgcatt atttattttt tctaagttcc agaataatag tgtcattatt
     5041 atagactata tgttttgaag tttgatatta taatgggata ttcatttttt gttcttttct
     5101 tgactccttt ctcaagtgtg tgataaggtc tgctgataaa atatttaacc ccaagaaagt
     5161 gaaaactaat ataaaattag aaagacctat ccaaattaga cagtcaattc cattaaaata
     5221 agaagtgaga aaaacaatgt tgggcattga ggtgtaaatt ttgcccagat gtatacccag
     5281 tgtgaaatat cttctaataa aaatatattt ggctcttatc cctgcacatg tagaggcata
     5341 aaaattggta aacatgtccc gctgtgtaga actttaaaaa aaaggcattt ttgaaagtgt
     5401 tgagtggcac tgataactgg tgaagcctac agccatccgc ccaaaagtct gttctgatgg
     5461 cactgagttt tcattgttct ggatgtataa gtctgtgtgt caggtacagc tgggcccagc
     5521 cagcttgagt cactcttgta caagcttgtt tttttctgtc ttgtgaatgc acttgataat
     5581 ttaaaaataa aaatatctgt ttctctgcag tttcagctaa taattttgag atatgtgttg
     5641 attttctgat tgaatcacca agaatttcaa caagtttaga aataatttaa gatgaatgaa
     5701 aaatatgtca gggggagcta agcatttgct aagctcttga tgtttatatg aaaaataaag
     5761 ggttgttttt atattgcaac tactaaagag aaaaatagca gttgaagcaa acaccaggaa
     5821 aatttggctc tactgaaagc ccagtccttt tcccctttgt ctcgctgaca aagtaagact
     5881 agggttatgt tcatgaatag attaataaaa gatgtttctt taccacacaa tctgagatga
     5941 gatggggtgg gggagatcat gacagtgccc tccaagcaat agatccagct gataatcaag
     6001 cagaaaatca gacaccagaa tccatttgat gatgtacggc attaaacatt tccttataac
     6061 aaa
//
