LOCUS       NM_001323342            8887 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens AT-hook containing transcription factor 1 (AHCTF1),
            transcript variant 2, mRNA.
ACCESSION   NM_001323342 XM_006711759
VERSION     NM_001323342.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   James,C., Moller,U., Spillner,C., Konig,S., Dybkov,O., Urlaub,H.,
            Lenz,C. and Kehlenbach,R.H.
  TITLE     Phosphorylation of ELYS promotes its interaction with VAPB at
            decondensing chromosomes during mitosis
  JOURNAL   EMBO Rep 25 (5), 2391-2417 (2024)
   PUBMED   38605278
  REMARK    GeneRIF: Phosphorylation of ELYS promotes its interaction with VAPB
            at decondensing chromosomes during mitosis.
REFERENCE   2  (bases 1 to 8887)
  AUTHORS   Chachoua,I., Tzelepis,I., Dai,H., Lim,J.P.,
            Lewandowska-Ronnegren,A., Casagrande,F.B., Wu,S., Vestlund,J.,
            Mallet de Lima,C.D., Bhartiya,D., Scholz,B.A., Martino,M.,
            Mehmood,R. and Gondor,A.
  TITLE     Canonical WNT signaling-dependent gating of MYC requires a
            noncanonical CTCF function at a distal binding site
  JOURNAL   Nat Commun 13 (1), 204 (2022)
   PUBMED   35017527
  REMARK    GeneRIF: Canonical WNT signaling-dependent gating of MYC requires a
            noncanonical CTCF function at a distal binding site.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8887)
  AUTHORS   Scholz,B.A., Sumida,N., de Lima,C.D.M., Chachoua,I., Martino,M.,
            Tzelepis,I., Nikoshkov,A., Zhao,H., Mehmood,R., Sifakis,E.G.,
            Bhartiya,D., Gondor,A. and Ohlsson,R.
  TITLE     WNT signaling and AHCTF1 promote oncogenic MYC expression through
            super-enhancer-mediated gene gating
  JOURNAL   Nat Genet 51 (12), 1723-1731 (2019)
   PUBMED   31784729
  REMARK    GeneRIF: AHCTF1 regulates the super-enhancer-mediated gene gating
            process.The cancer-cell-specific gating of MYC is regulated by
            AHCTF1, which connects nucleoporins to the oncogenic super-enhancer
            via beta-catenin.
            Erratum:[Nat Genet. 2020 Nov;52(11):1265. doi:
            10.1038/s41588-020-00715-9.. PMID: 32948851]
REFERENCE   4  (bases 1 to 8887)
  AUTHORS   Jevtic,P., Schibler,A.C., Wesley,C.C., Pegoraro,G., Misteli,T. and
            Levy,D.L.
  TITLE     The nucleoporin ELYS regulates nuclear size by controlling NPC
            number and nuclear import capacity
  JOURNAL   EMBO Rep 20 (6) (2019)
   PUBMED   31085625
  REMARK    GeneRIF: we identify ELYS as a novel positive effector of mammalian
            nuclear size and propose that nuclear size is sensitive to NPC
            density and nuclear import capacity.
REFERENCE   5  (bases 1 to 8887)
  AUTHORS   Mimura,Y., Takagi,M., Clever,M. and Imamoto,N.
  TITLE     ELYS regulates the localization of LBR by modulating its
            phosphorylation state
  JOURNAL   J Cell Sci 129 (22), 4200-4212 (2016)
   PUBMED   27802161
  REMARK    GeneRIF: These observations point to a new mechanism regulating the
            localization of LBR, which is governed by an ELYS-mediated
            phosphorylation network.
REFERENCE   6  (bases 1 to 8887)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   7  (bases 1 to 8887)
  AUTHORS   Rasala,B.A., Orjalo,A.V., Shen,Z., Briggs,S. and Forbes,D.J.
  TITLE     ELYS is a dual nucleoporin/kinetochore protein required for nuclear
            pore assembly and proper cell division
  JOURNAL   Proc Natl Acad Sci U S A 103 (47), 17801-17806 (2006)
   PUBMED   17098863
  REMARK    GeneRIF: Results suggets that ELYS is an unexpected member of the
            nuclear pore and kinetochore that functions in both pore assembly
            at the nucleus and faithful cell division.
REFERENCE   8  (bases 1 to 8887)
  AUTHORS   Beausoleil,S.A., Villen,J., Gerber,S.A., Rush,J. and Gygi,S.P.
  TITLE     A probability-based approach for high-throughput protein
            phosphorylation analysis and site localization
  JOURNAL   Nat Biotechnol 24 (10), 1285-1292 (2006)
   PUBMED   16964243
REFERENCE   9  (bases 1 to 8887)
  AUTHORS   Nousiainen,M., Sillje,H.H., Sauer,G., Nigg,E.A. and Korner,R.
  TITLE     Phosphoproteome analysis of the human mitotic spindle
  JOURNAL   Proc Natl Acad Sci U S A 103 (14), 5391-5396 (2006)
   PUBMED   16565220
REFERENCE   10 (bases 1 to 8887)
  AUTHORS   Kimura,N., Takizawa,M., Okita,K., Natori,O., Igarashi,K., Ueno,M.,
            Nakashima,K., Nobuhisa,I. and Taga,T.
  TITLE     Identification of a novel transcription factor, ELYS, expressed
            predominantly in mouse foetal haematopoietic tissues
  JOURNAL   Genes Cells 7 (4), 435-446 (2002)
   PUBMED   11952839
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC113174.3.
            
            On Jun 2, 2019 this sequence version replaced NM_001323342.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.2249526.1,
                                           SRR14038192.1639088.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000648844.2/ ENSP00000497061.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-371               AC113174.3         122659-123029       c
            372-499             AC113174.3         109331-109458       c
            500-753             AC113174.3         107223-107476       c
            754-934             AC113174.3         104313-104493       c
            935-1142            AC113174.3         98632-98839         c
            1143-1259           AC113174.3         96622-96738         c
            1260-1344           AC113174.3         95030-95114         c
            1345-1495           AC113174.3         93606-93756         c
            1496-1628           AC113174.3         91418-91550         c
            1629-1810           AC113174.3         91146-91327         c
            1811-1872           AC113174.3         90532-90593         c
            1873-2001           AC113174.3         89289-89417         c
            2002-2092           AC113174.3         86916-87006         c
            2093-2182           AC113174.3         85740-85829         c
            2183-2323           AC113174.3         82860-83000         c
            2324-2428           AC113174.3         82037-82141         c
            2429-2522           AC113174.3         81047-81140         c
            2523-2646           AC113174.3         79475-79598         c
            2647-2703           AC113174.3         79258-79314         c
            2704-2850           AC113174.3         78292-78438         c
            2851-3038           AC113174.3         76574-76761         c
            3039-3183           AC113174.3         68239-68383         c
            3184-3315           AC113174.3         68031-68162         c
            3316-3466           AC113174.3         67118-67268         c
            3467-3617           AC113174.3         58742-58892         c
            3618-3725           AC113174.3         58325-58432         c
            3726-3918           AC113174.3         55005-55197         c
            3919-4113           AC113174.3         53040-53234         c
            4114-4510           AC113174.3         51980-52376         c
            4511-4634           AC113174.3         48772-48895         c
            4635-4732           AC113174.3         46811-46908         c
            4733-4941           AC113174.3         44172-44380         c
            4942-6769           AC113174.3         40696-42523         c
            6770-6903           AC113174.3         34876-35009         c
            6904-6986           AC113174.3         33775-33857         c
            6987-8887           AC113174.3         30179-32079         c
FEATURES             Location/Qualifiers
     source          1..8887
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q44"
     gene            1..8887
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /note="AT-hook containing transcription factor 1"
                     /db_xref="GeneID:25909"
                     /db_xref="HGNC:HGNC:24618"
                     /db_xref="MIM:610853"
     exon            1..371
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            372..499
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     CDS             379..7179
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /note="isoform 2 is encoded by transcript variant 2;
                     protein ELYS; ELYS transcription factor-like protein
                     TMBS62; embryonic large molecule derived from yolk sac;
                     putative AT-hook-containing transcription factor 1"
                     /codon_start=1
                     /product="protein ELYS isoform 2"
                     /protein_id="NP_001310271.1"
                     /db_xref="CCDS:CCDS91190.1"
                     /db_xref="GeneID:25909"
                     /db_xref="HGNC:HGNC:24618"
                     /db_xref="MIM:610853"
                     /translation="MRDLRAQVTSGLLPFPEVTLQALGEDEITLESVLRGKFAAGKNG
                     LACLACGPQLEVVNSITGERLSAYRFSGVNEQPPVVLAVKEFSWQKRTGLLIGLEETE
                     GSVLCLYDLGISKVVKAVVLPGRVTAIEPIINHGGASASTQHLHPSLRWLFGVAAVVT
                     DVGQILLVDLCLDDLSCNQNEVEASDLEVLTGIPAEVPHIRESVMRQGRHLCFQLVSP
                     TGTAVSTLSYISRTNQLAVGFSDGYLALWNMKSMKREYYIQLESGQVPVYAVTFQEPE
                     NDPRNCCYLWAVQSTQDSEGDVLSLHLLQLAFGNRKCLASGQILYEGLEYCEERYTLD
                     LTGGMFPLRGQTSNTKLLGCQSIEKFRSHGDREEGVNEALSPDTSVSVFTWQVNIYGQ
                     GKPSVYLGLFDINRWYHAQMPDSLRSGEYLHNCSYFALWSLESVVSRTSPHGILDILV
                     HERSLNRGVPPSYPPPEQFFNPSTYNFDATCLLNSGVVHLTCTGFQKETLTFLKKSGP
                     SLNELIPDGYNRCLVAGLLSPRFVDVQPSSLSQEEQLEAILSAAIQTSSLGLLTGYIR
                     RWITEEQPNSATNLRFVLEWTWNKVVLTKEEFDRLCVPLFDGSCHFMDPQTIQSIQQC
                     YLLLSNLNIVLSCFASEAREITERGLIDLSNKFVVSHLICQYAQVVLWFSHSGLLPEG
                     IDDSVQLSRLCYNYPVIQNYYTSRRQKFERLSRGKWNPDCLMIDGLVSQLGERIEKLW
                     KRDEGGTGKYPPASLHAVLDMYLLDGVTEAAKHSITIYLLLDIMYSFPNKTDTPIESF
                     PTVFAISWGQVKLIQGFWLIDHNDYESGLDLLFHPATAKPLSWQHSKIIQAFMSQGEH
                     RQALRYIQTMKPTVSSGNDVILHLTVLLFNRCMVEAWNFLRQHCNRLNIEELLKHMYE
                     VCQEMGLMEDLLKLPFTDTEQECLVKFLQSSASVQNHEFLLVHHLQRANYVPALKLNQ
                     TLKINVMNDRDPRLRERSLARNSILDQYGKILPRVHRKLAIERAKPYHLSTSSVFRLV
                     SRPKPLSAVPKQVVTGTVLTRSVFINNVLSKIGEVWASKEPINSTTPFNSSKIEEPSP
                     IVYSLPAPELPEAFFGTPISKASQKISRLLDLVVQPVPRPSQCSEFIQQSSMKSPLYL
                     VSRSLPSSSQLKGSPQAISRASELHLLETPLVVKKAKSLAMSVTTSGFSEFTPQSILR
                     STLRSTPLASPSPSPGRSPQRLKETRISFVEEDVHPKWIPGAADDSKLEVFTTPKKCA
                     VPVETEWLKSKDRTTSFFLNSPEKEHQEMDEGSQSLEKLDVSKGNSSVSITSDETTLE
                     YQDAPSPEDLEETVFTASKPKSSSTALTTNVTEQTEKDGDKDVFASEVTPSDLQKQMG
                     NLEDAETKDLLVAAEAFSELNHLSPVQGTEASLCAPSVYEGKIFTQKSKVPVLDEGLT
                     SVETYTPAIRANDNKSMADVLGDGGNSSLTISEGPIVSERRLNQEVALNLKEDHEVEV
                     GVLKESVDLPEEKLPISDSPPDTQEIHVIEQEKLEAQDSGEEARNLSFNELYPSGTLK
                     LQYNFDTIDQQFCDLADNKDTAECDIAEVDGELFVAQSNFTLILEGEEGEVEPGDFAS
                     SDVLPKAANTATEEKLVCSGENDNHGQIANLPSAVTSDQKSQKVDTLPYVPEPIKVAI
                     AENLLDVIKDTRSKEITSDTMEQSIHETIPLVSQNIMCPTKLVKSAFKTAQETSTMTM
                     NVSQVDDVVSSKTRTRGQRIQNVNVKSAQQEASADVATPKMPGQSVRKKTRKAKEISE
                     ASENIYSDVRGLSQNQQIPQNSVTPRRGRRKKEVNQDILENTSSVEQELQITTGRESK
                     RLKSSQLLEPAVEETTKKEVKVSSVTKRTPRRIKRSVENQESVEIINDLKVSTVTSPS
                     RMIRKLRSTNLDASENTGNKQDDKSSDKQLRIKHVRRVRGREVSPSDVREDSNLESSQ
                     LTVQAEFDMSAIPRKRGRPRKINPSEDVGSKAVKEERSPKKKEAPSIRRRSTRNTPAK
                     SENVDVGKPALGKSILVPNEELSMVMSSKKKLTKKTESQSQKRSLHSVSEERTDEMTH
                     KETNEQEERLLATASFTKSSRSSRTRSSKAILLPDLSEPNNEPLFSPASEVPRKAKAK
                     KIEVPAQLKELVSDLSSQFVISPPALRSRQKNTSNKNKLEDELKDDAQSVETLGKPKA
                     KRIRTSKTKQASKNTEKESAWSPPPIEIRLISPLASPADGVKSKPRKTTEVTGTGLGR
                     NRKKLSSYPKQILRRKML"
     exon            500..753
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            754..934
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1142
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            1143..1259
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            1260..1344
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            1345..1495
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            1496..1628
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            1629..1810
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            1811..1872
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            1873..2001
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            2002..2092
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            2093..2182
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            2183..2323
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            2324..2428
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            2429..2522
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            2523..2646
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            2647..2703
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            2704..2850
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            2851..3038
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            3039..3183
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            3184..3315
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            3316..3466
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            3467..3617
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            3618..3725
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            3726..3918
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            3919..4113
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            4114..4510
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            4511..4634
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            4635..4732
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            4733..4941
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            4942..6769
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            6770..6903
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            6904..6986
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     exon            6987..8887
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7424..7429
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /note="hexamer: AATAAA"
     polyA_site      7446
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /note="major polyA site"
     regulatory      8649..8654
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /note="hexamer: AATAAA"
     polyA_site      8669
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
     regulatory      8865..8870
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
                     /note="hexamer: AATAAA"
     polyA_site      8887
                     /gene="AHCTF1"
                     /gene_synonym="ELYS; MST108; MSTP108; TMBS62"
ORIGIN      
        1 ggttttgtcg gcgcagggta atgcgcagag cggggccggc gctgtccgag gcgagcaggc
       61 agcgttgcaa ggggaaggac ccagaagcgc ggcgggagca gcgactgcgt tttagccgct
      121 gagcggtctg caggcctaca gcccgcgaag gcactctcgg gggcgtgtgg ctctgaggtg
      181 gccggggagg tcgcaggcgg cggccggagc gcagaggagc ggcttcagcc gggcggaggg
      241 gtgtgagccc ggcccgtggc ggccacgtct cccgggagat gctgtgacgg acccgcacgg
      301 gaggagctcg cgcctggcct ggcgacccgg tggactcggc ccgcggcggc gcgctcaccc
      361 gccccgttca gtggaagtat gcgagactta agagctcaag tgactagtgg tctcctgcca
      421 tttccagaag tgactcttca agcccttgga gaagacgaaa taacattaga atctgtgctt
      481 cgtggaaagt ttgctgcagg gaaaaatgga cttgcttgct tggcttgtgg tccacaactt
      541 gaggtagtaa actctataac aggagagcga ttgtctgctt acagattcag tggagtcaat
      601 gaacagcctc ctgtagtttt agctgtgaaa gaattctctt ggcagaagag aactggatta
      661 ttaataggat tggaagaaac agaagggagt gttctctgtc tttatgacct tggaatatca
      721 aaagtagtta aagcagttgt tcttcctgga agggtaacag ctattgaacc tataattaat
      781 catggaggag ccagtgcaag cactcagcat ttacatccaa gtctgcgatg gctttttgga
      841 gtggcagctg tggtcactga tgttggacag atccttcttg ttgacctatg tttggatgac
      901 ttgtcatgca atcaaaatga agttgaagca tcagatcttg aagttctaac tggtatccca
      961 gctgaagtac cacacattag agaaagtgta atgagacaag ggcgccatct gtgtttccag
     1021 ttagtaagtc caacaggaac agctgtttca actcttagtt acataagcag gacaaatcag
     1081 cttgctgtag gtttttctga tggctatcta gcactttgga acatgaaaag catgaaaaga
     1141 gaatattaca tacaattgga aagtggacaa gttcctgtat atgctgtcac ttttcaagaa
     1201 cctgagaatg atcctcggaa ttgctgttac ttgtgggctg ttcagtctac acaagatagt
     1261 gaaggggatg ttttgagttt gcatctgctg cagctggcct ttggtaatag aaagtgtttg
     1321 gcatcaggac aaatcttata tgaggggtta gaatactgtg aagaaagata caccctggac
     1381 ctgacaggtg gcatgttccc tttgagggga cagacgagta ataccaaatt gttgggatgc
     1441 cagagtatag agaaatttcg atctcatggt gacagggagg aaggcgtgaa tgaagctcta
     1501 tcgcctgaca ctagtgtttc agtctttacc tggcaggtga atatatatgg acagggaaag
     1561 ccttctgtat atttggggct ttttgatata aatcgttggt atcatgcaca aatgccagat
     1621 tcgttaaggt caggagaata tctacataat tgctcttatt ttgcactgtg gtcattggag
     1681 tctgttgtaa gtaggacttc tccacatggc atcttggata tattagtaca tgagagaagt
     1741 ttaaatagag gagtccctcc ttcatatcca cctcccgagc agttttttaa tccaagcact
     1801 tataattttg atgccacttg tttgttaaac tcgggagttg ttcatttaac ttgtactggc
     1861 tttcagaagg agactttgac ttttttaaag aaatcaggtc catcactcaa tgaactcatt
     1921 cctgatggtt ataatcgatg tcttgtagct ggccttcttt ccccaagatt tgttgatgtt
     1981 cagccttcca gtttaagcca agaagaacag ttagaagcta tattgtcagc agcaattcag
     2041 actagttccc tgggactttt gactggttat atccgaagat ggataacaga agaacaacca
     2101 aattctgcca ctaatttgcg ctttgttctt gaatggacgt ggaataaagt ggttctcaca
     2161 aaagaggaat ttgacagact atgtgtgcca ttatttgatg gttcgtgtca tttcatggat
     2221 ccacaaacta tacagtctat ccagcaatgc tatttgcttc ttagcaatct taatatagtc
     2281 ttgagctgtt ttgcatcaga agcccgagag atcactgaga gaggactgat agacttaagc
     2341 aataagtttg tggtttccca cctcatctgt cagtatgcac aagtggttct ttggttctct
     2401 cattctgggc ttttaccaga aggcatagat gattctgtgc agttgtcaag gttatgctac
     2461 aactaccctg taattcagaa ctactacacc agtcgtcgac agaagtttga gcgtttatca
     2521 agagggaagt ggaatcccga ttgcttgatg attgatggac tggtttctca gttaggagag
     2581 cgaattgaga agttgtggaa acgagatgaa ggaggcacag gaaaatatcc tcctgctagt
     2641 ctgcatgcag tacttgatat gtacctatta gacggcgtta ctgaagcagc caaacactct
     2701 attaccattt atttgctact tgatattatg tattcctttc ccaacaaaac agacactccc
     2761 attgaatctt tcccaactgt atttgccatt tcttggggcc aagttaaact tattcagggg
     2821 ttttggttga tagatcataa tgactatgag agtggtttgg atcttttgtt tcatccagct
     2881 actgcaaaac ctttgtcatg gcaacattca aagattattc aggcattcat gagtcagggc
     2941 gagcacagac aagccctcag atatattcag acaatgaagc caacagtgtc cagtggtaac
     3001 gatgttatcc ttcacctcac tgttttgctt tttaataggt gtatggttga agcctggaat
     3061 tttttgcggc aacattgcaa taggttgaat atagaggagt tactgaagca catgtatgaa
     3121 gtctgtcagg aaatgggctt gatggaagat ttactgaagt taccatttac agacactgag
     3181 caggaatgtt tagtgaaatt tttgcagtcc agtgccagcg ttcagaatca tgaattcctt
     3241 ttagtgcacc atttgcagcg tgccaattat gtgcctgcct tgaagctgaa ccaaactctg
     3301 aagattaatg ttatgaatga tcgtgatcct cgtttgcggg agagatcact ggctcgaaat
     3361 tctatattag accagtatgg aaaaatcctt cctagagtcc atcgaaaatt agccattgaa
     3421 cgagctaagc cttatcatct gtcaacatca tcagtttttc gattagtttc tagacccaaa
     3481 ccattatcag cagttccaaa gcaagttgta acaggaactg tgttgacaag atctgttttc
     3541 atcaacaatg tgttatctaa aattggagaa gtttgggcaa gcaaagaacc tataaatagc
     3601 accacacctt tcaatagttc taaaatagaa gaaccatctc ctatagtgta ttcgctccca
     3661 gctccagagc tgcctgaggc attttttgga acaccaattt caaaagcatc acaaaaaatt
     3721 tctagactgc tagatttggt tgttcagcct gtcccccggc cttctcagtg ttcggagttt
     3781 attcagcaaa gctccatgaa atctcctttg tacctagtat cccgttcact gccctcaagt
     3841 tcgcaattaa aaggatcgcc tcaggccatc tccagggctt cagaattaca tttgcttgaa
     3901 actcctcttg tagttaagaa agctaaaagt ttggccatgt cagttactac ttctggattt
     3961 tctgagttca ctcctcagtc catcctgagg tctactcttc gatcaacacc tttagcatct
     4021 ccctctccat cacctggaag gtctcctcaa cgacttaaag aaactagaat ttcatttgtg
     4081 gaagaagatg tccacccaaa atggattcct ggggctgcag atgatagcaa attagaagta
     4141 tttactacac ctaaaaaatg tgcagttcca gtggaaactg aatggctgaa gagcaaagat
     4201 aggaccacat cttttttcct gaacagccct gaaaaggagc atcaagaaat ggatgagggg
     4261 tcacaaagtt tagagaaact ggatgtgagc aaaggaaaca gcagtgtttc aatcacatcc
     4321 gatgagacta ccttagagta tcaggatgca ccgtcaccgg aagaccttga agagactgtt
     4381 ttcacggcct ctaagcccaa aagctcttcc actgcactaa ctactaatgt aactgaacaa
     4441 actgaaaagg atggagataa agatgtattt gcatcagaag taactccttc agacctacag
     4501 aaacaaatgg gcaatttaga agatgcagaa acaaaggatc tcttagttgc agcagaggca
     4561 ttttcagaat tgaatcactt aagcccggtt caaggaactg aagcttctct ttgtgcacca
     4621 tcagtctatg aagggaaaat cttcacccag aagtccaagg taccagtgtt ggacgaagga
     4681 ttaacatctg ttgaaaccta cacccctgca attagagcaa atgacaataa atctatggct
     4741 gatgtccttg gtgatggtgg aaactcctcg ctcactatct ctgaaggtcc tattgtctct
     4801 gagcgcaggc ttaaccagga agtagcgctg aacttaaaag aagatcatga agtagaagtt
     4861 ggtgtactaa aagaaagtgt tgacttacca gaagaaaagc ttccaatttc tgacagccct
     4921 cctgatactc aagaaattca tgtgattgaa caagaaaagc ttgaagctca agattcagga
     4981 gaagaggcta ggaatctttc atttaatgag ttatatccct ctggaacact taagcttcag
     5041 tacaattttg atactattga ccaacagttt tgtgacttag ctgataacaa agacactgct
     5101 gaatgtgaca ttgctgaagt agatggggaa ctttttgtgg ctcaaagcaa ctttaccttg
     5161 atattggaag gtgaagaagg agaagttgag ccaggtgatt ttgcatcatc tgatgtgtta
     5221 cctaaagcag ctaacacagc aactgaagaa aaacttgtat gcagtgggga aaatgataat
     5281 catggacaaa ttgcaaattt gccatctgcc gtaactagtg accaaaagtc ccaaaaagta
     5341 gacactttac catatgtgcc tgaacctatt aaagtagcaa ttgcagaaaa tttactagat
     5401 gtaattaaag acacaagaag taaagaaatt acttcagata caatggaaca gtccattcat
     5461 gaaacaatac ctttagtgag ccaaaacata atgtgtccca ctaaattggt caaatctgca
     5521 tttaagactg ctcaggaaac aagcacaatg actatgaatg tcagccaggt tgatgacgtg
     5581 gtttcctcca aaactcgtac gagaggtcaa cgtatccaaa acgtgaatgt caaatcagca
     5641 caacaggaag catcagcaga tgttgctact cctaagatgc cagggcagtc agtcaggaag
     5701 aaaactagga aggcaaaaga aatttctgaa gcttctgaaa acatctattc tgatgtcaga
     5761 ggactatctc agaaccagca aatacctcaa aattctgtta cgcctaggag aggaaggaga
     5821 aagaaagaag ttaatcagga catactagaa aacaccagtt ctgtggaaca agaattacag
     5881 atcactacag gtagggaatc aaaaagatta aaatcatctc agctgttgga accagcagtt
     5941 gaagaaacta ctaaaaaaga agttaaggtt tcatctgtta caaaaaggac tcctagaaga
     6001 attaaaagat ctgtagaaaa tcaggaaagt gttgaaatta taaatgatct aaaagttagt
     6061 acggtaacaa gtcctagcag aatgatcaga aaattgagaa gtactaattt agatgcttct
     6121 gaaaatacag gaaataagca agatgataaa tccagtgaca agcagctgcg tattaaacat
     6181 gttagaaggg tcagagggag agaagttagt ccatcagatg tgagagaaga ctccaacctt
     6241 gagtcatctc agttgactgt tcaagcagaa tttgatatgt ctgccatacc tagaaaacgt
     6301 ggtagaccaa gaaaaatcaa tccatctgaa gatgtaggat ctaaggctgt taaggaagag
     6361 agaagcccca agaagaaaga agctcccagc attagaagga gatctacaag aaatacccca
     6421 gctaaaagtg aaaatgttga tgttggaaaa ccagctttag gaaaatccat tttagtgcca
     6481 aacgaggaac tttcgatggt gatgagctct aagaaaaaac ttacaaaaaa gactgaaagt
     6541 caaagccaaa aacgttcatt gcactcagta tcagaagaac gcacagatga aatgacacat
     6601 aaagaaacaa atgagcagga agaaagattg ctcgccacag cttccttcac taaatcatcc
     6661 cgcagcagca ggactcggtc tagcaaggcc atcttgttgc cggacctttc tgaaccaaac
     6721 aatgagcctt tattttctcc agcgtcagaa gttccaagga aagcaaaagc taaaaaaata
     6781 gaggttcctg cacagctgaa agaattagtt tcggatttat cttctcagtt tgtcatctca
     6841 cctcctgctt taaggagcag acaaaaaaac acatccaata agaacaagct tgaagatgaa
     6901 ctgaaagatg atgcacaatc agtagaaact ctgggaaagc caaaagcgaa acgaatcagg
     6961 acgtcaaaaa caaaacaagc aagcaaaaac acagaaaaag aaagtgcttg gtcacctcct
     7021 cccatagaaa ttcggctgat ttcccccttg gctagcccag ctgacggagt caagagcaaa
     7081 ccaagaaaaa ctacagaagt gacaggaaca ggtcttggaa ggaacagaaa gaaactgtct
     7141 tcctatccaa agcaaatttt acgcagaaaa atgctgtaat ttcttgggaa gattttaatg
     7201 tacacctatt tgtaaagtca tcagaatagt gtggattatt aaatatctag tttggaagaa
     7261 aataatttat ataaattatt gtaaattttt atgtaaacag aaggtcttca ataagtaaag
     7321 taactccata tggagtgatt gtttcagtcc aggcaatttt tctattttat attaagactt
     7381 catacattta tatatgtaaa tatggcttat taatggaatg ttaaataaaa tgtatacttc
     7441 acagtcgttt gtgtcttgga tttttgaaag ggaggggata tctgtttaaa tagttttata
     7501 tgctcattgg tctcattttc tctataatta aaatactaga ccagtcttaa aatggggatg
     7561 attgaagtat tgatatttct ttttacagtt actattttat aatttatgca ctttgattct
     7621 gtgattcaga tttctaatca gaaaatgtat ttttttgttt ttggctgtta ctatgttaaa
     7681 attgaattat gggcatgtca ttttgccatc tttgtagttt cacaaatttt gtgtaatcta
     7741 cctcaaatga ataatccaag tattggttaa ctataatgtt ggcatctctt attcggcaag
     7801 cttaaaggct ctttaaagtc ttaattagtc aaagactaat ccaggttaga ttgaccggtt
     7861 cactgctcac ttgcaacctt atcaaagggt ttgacaaagg gaaatgtaaa ataaatctgt
     7921 ttatggatat tgagtgcatc ttgtatgtgc ctaatattga taggatgaga tgtctgaaca
     7981 aatttttata atattgctgt gaaggagctt gctattgaac cacagaaatc ccttaatatt
     8041 caggttttaa aactggcaaa ttctcacagg acctcaggca cagattattg aggttgggag
     8101 agagtgagta gatgtagaaa aggagaaaaa caacacacgc cctgttctct acagtacaac
     8161 tgtgtgcaat taagcaatgg tacttgatgt aggctctaac actcatcaat aaataagtgt
     8221 tgtaaaataa tttataacag gtaatcgata gtgtgtaatg aatggactat taataattga
     8281 ttatctagaa acgaactgct ttcgtgggct tttaatattt taatgtgaag catatgcagt
     8341 gtgctttctg catttatttt tctaccaaat aatacagata atgagaaatt ggtgaaaatg
     8401 cctacgcaaa gtgttgacag tgtgaaagca gtgcgagtgc ggccttttag tcaggttagt
     8461 gatggatgtt acgctgcctt gttgaaaatt tcactgactt tgattttatt acttttttaa
     8521 tgatagttat caaacttgta tttaagctgc ttgtcattta tggaatattg aacttattta
     8581 aatgaacttg ttaaatgaat aaagagctaa acataattca gtaaacaatt cctttgcgca
     8641 agtagcacaa taaacatgga tgcaacgtat gtcaagttaa tactttttta aaccaacgca
     8701 atttggtgaa tatagatgtg tggtacctgt ttttaataag tgtacttttt ttcccccctc
     8761 cgtgaatgta gatcataagc aaacaaattg cctgttctaa atgaacttta catatatttt
     8821 aaatgaatgt atgtacttac gtataaatgt ctttatatag cttgaataaa aacactgctc
     8881 attaaaa
//
