LOCUS       NM_198576               7326 bp    mRNA    linear   PRI 07-OCT-2024
DEFINITION  Homo sapiens agrin (AGRN), transcript variant 2, mRNA.
ACCESSION   NM_198576 XM_372195
VERSION     NM_198576.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Kamiya,K., Tachiki,T., Sato,Y., Kouda,K., Kajita,E., Tamaki,J.,
            Kagamimori,S. and Iki,M.
  TITLE     Association between the 110-kDa C-terminal agrin fragment and
            skeletal muscle decline among community-dwelling older women
  JOURNAL   J Cachexia Sarcopenia Muscle 14 (5), 2253-2263 (2023)
   PUBMED   37562951
  REMARK    GeneRIF: Association between the 110-kDa C-terminal agrin fragment
            and skeletal muscle decline among community-dwelling older women.
REFERENCE   2  (bases 1 to 7326)
  AUTHORS   Lv,R., Duan,L., Gao,J., Si,J., Feng,C., Hu,J. and Zheng,X.
  TITLE     Bioinformatics-based analysis of the roles of basement
            membrane-related gene AGRN in systemic lupus erythematosus and
            pan-cancer development
  JOURNAL   Front Immunol 14, 1231611 (2023)
   PUBMED   37841281
  REMARK    GeneRIF: Bioinformatics-based analysis of the roles of basement
            membrane-related gene AGRN in systemic lupus erythematosus and
            pan-cancer development.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7326)
  AUTHORS   Uyen Dao,T.M., Barbeau,S., Messeant,J., Della-Gaspera,B.,
            Bouceba,T., Semprez,F., Legay,C. and Dobbertin,A.
  TITLE     The collagen ColQ binds to LRP4 and regulates the activation of the
            Muscle-Specific Kinase-LRP4 receptor complex by agrin at the
            neuromuscular junction
  JOURNAL   J Biol Chem 299 (8), 104962 (2023)
   PUBMED   37356721
  REMARK    GeneRIF: The collagen ColQ binds to LRP4 and regulates the
            activation of the Muscle-Specific Kinase-LRP4 receptor complex by
            agrin at the neuromuscular junction.
REFERENCE   4  (bases 1 to 7326)
  AUTHORS   Zhang,H., Liang,J., Lu,T., Li,M., Shan,G., Bi,G., Zhao,M., Jin,X.,
            Wang,Q., Chen,Z. and Zhan,C.
  TITLE     AGRN promotes lung adenocarcinoma progression by activating Notch
            signaling pathway and acts as a therapeutic target
  JOURNAL   Pharmacol Res 194, 106819 (2023)
   PUBMED   37321467
  REMARK    GeneRIF: AGRN promotes lung adenocarcinoma progression by
            activating Notch signaling pathway and acts as a therapeutic
            target.
REFERENCE   5  (bases 1 to 7326)
  AUTHORS   Pratt,J., Whitton,L., Ryan,A., Juliusdottir,T., Dolan,J.,
            Conroy,J., Narici,M., De Vito,G. and Boreham,C.
  TITLE     Genes encoding agrin (AGRN) and neurotrypsin (PRSS12) are
            associated with muscle mass, strength and plasma C-terminal agrin
            fragment concentration
  JOURNAL   Geroscience 45 (3), 1289-1302 (2023)
   PUBMED   36609795
  REMARK    GeneRIF: Genes encoding agrin (AGRN) and neurotrypsin (PRSS12) are
            associated with muscle mass, strength and plasma C-terminal agrin
            fragment concentration.
REFERENCE   6  (bases 1 to 7326)
  AUTHORS   Denzer,A.J., Brandenberger,R., Gesemann,M., Chiquet,M. and
            Ruegg,M.A.
  TITLE     Agrin binds to the nerve-muscle basal lamina via laminin
  JOURNAL   J Cell Biol 137 (3), 671-683 (1997)
   PUBMED   9151673
REFERENCE   7  (bases 1 to 7326)
  AUTHORS   Abicht,A., Muller,J.S. and Lochmuller,H.
  TITLE     Congenital Myasthenic Syndromes Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301347
REFERENCE   8  (bases 1 to 7326)
  AUTHORS   Rupp,F., Ozcelik,T., Linial,M., Peterson,K., Francke,U. and
            Scheller,R.
  TITLE     Structure and chromosomal localization of the mammalian agrin gene
  JOURNAL   J Neurosci 12 (9), 3535-3544 (1992)
   PUBMED   1326608
REFERENCE   9  (bases 1 to 7326)
  AUTHORS   Campanelli,J.T., Hoch,W., Rupp,F., Kreiner,T. and Scheller,R.H.
  TITLE     Agrin mediates cell contact-induced acetylcholine receptor
            clustering
  JOURNAL   Cell 67 (5), 909-916 (1991)
   PUBMED   1659950
REFERENCE   10 (bases 1 to 7326)
  AUTHORS   Rupp,F., Payan,D.G., Magill-Solc,C., Cowan,D.M. and Scheller,R.H.
  TITLE     Structure and expression of a rat agrin
  JOURNAL   Neuron 6 (5), 811-823 (1991)
   PUBMED   1851019
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL645608.30, AB191264.1,
            CN314674.1, CN314678.1, AW119098.1, BC007649.1 and CK000983.1.
            
            On Nov 22, 2018 this sequence version replaced NM_198576.3.
            
            Summary: This gene encodes one of several proteins that are
            critical in the development of the neuromuscular junction (NMJ), as
            identified in mouse knock-out studies. The encoded protein contains
            several laminin G, Kazal type serine protease inhibitor, and
            epidermal growth factor domains. Additional post-translational
            modifications occur to add glycosaminoglycans and disulfide bonds.
            In one family with congenital myasthenic syndrome affecting
            limb-girdle muscles, a mutation in this gene was found. Alternative
            splicing results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Mar 2015].
            
            Transcript Variant: This variant (2) lacks alternate in-frame exons
            in the 3' coding region, compared to variant 1. The encoded isoform
            (2) is shorter, compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB191264.1, AF016903.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000379370.7/ ENSP00000368678.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 AL645608.30        143970-143972
            4-20                AB191264.1         1-17
            21-98               CN314674.1         1-78
            99-2964             AB191264.1         96-2961
            2965-3119           CN314678.1         88-242
            3120-3441           AB191264.1         3117-3438
            3442-3611           AW119098.1         164-333             c
            3612-4723           AB191264.1         3609-4720
            4724-6033           BC007649.1         1-1310
            6034-6966           AL645608.30        178674-179606
            6967-7326           CK000983.1         7-366
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.33"
     gene            1..7326
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /note="agrin"
                     /db_xref="GeneID:375790"
                     /db_xref="HGNC:HGNC:329"
                     /db_xref="MIM:103320"
     exon            1..254
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     CDS             54..6191
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; agrin proteoglycan"
                     /codon_start=1
                     /product="agrin isoform 2 precursor"
                     /protein_id="NP_940978.2"
                     /db_xref="CCDS:CCDS30551.1"
                     /db_xref="GeneID:375790"
                     /db_xref="HGNC:HGNC:329"
                     /db_xref="MIM:103320"
                     /translation="MAGRSHPGPLRPLLPLLVVAACVLPGAGGTCPERALERREEEAN
                     VVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESLLDGGNKVVISGFGDPLI
                     CDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGTH
                     FTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPSVVAPVCGSDASTYS
                     NECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPATC
                     RGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVN
                     PRTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGR
                     DQCPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQ
                     RAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSDGVT
                     YGSACELEATACTLGREIQVARKGPCDRCGQCRFGALCEAETGRCVCPSECVALAQPV
                     CGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQCVCPRCE
                     HPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDC
                     EQELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQR
                     GLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPH
                     GSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVR
                     DDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADASAPATCAEMRCEFGARCVE
                     ESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQEA
                     VAPSTHPTSASVTVTTPGLLLSQALPAPPGALPLAPSSTAHSQTTPPPSSRPRTTASV
                     PRTTVWPVLTVPPTAPSPAPSLVASAFGESGSTDGSSDEELSGDQEASGGGSGGLEPL
                     EGSSVATPGPPVERASCYNSALGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQ
                     ELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFRSVRLRDLGPGKSVRAIV
                     DVHFDPTTAFRAPDVARALLRQIQVSRRRSLGVRRPLQEHVRFMDFDWFPAFITGATS
                     GAIAAGATARATTASRLPSSAVTPRAPHPSHTSQPVAKTTAAPTTRRPPTTAPSRVPG
                     RRPPAPQQPPKPCDSQPCFHGGTCQDWALGGGFTCSCPAGRGGAVCEKVLGAPVPAFE
                     GRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLALALLDGRVQLRFDT
                     GSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLF
                     VGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHP
                     CLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPCQPNPCHGAAPCRVLPEGG
                     AQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVF
                     LARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTR
                     VSLERNGRKGALRVGDGPRVLGESPVPHTVLNLKEPLYVGGAPDFSKLARAAAVSSGF
                     DGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLNGASCVPREAAYVCL
                     CPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESEKALQSNHFELSLRTE
                     ATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAH
                     REQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGC
                     LRDVVVGRHPLHLLEDAVTKPELRPCPTP"
     exon            255..516
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            517..564
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            565..780
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            781..1005
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1230
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1437
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            1438..1656
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            1657..1851
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            1852..2052
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            2053..2201
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            2202..2307
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            2308..2424
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            2425..2589
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            2590..2733
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            2734..2858
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            2859..2964
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            2965..3303
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            3304..3441
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            3442..3569
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            3570..3684
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            3685..3804
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            3805..4158
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            4159..4351
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            4352..4567
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            4568..4797
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            4798..4932
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            4933..5029
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            5030..5194
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            5195..5306
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            5307..5423
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            5424..5616
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            5617..5704
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            5705..5929
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            5930..6033
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     exon            6034..7326
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7304..7309
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /note="hexamer: AATAAA"
     polyA_site      7326
                     /gene="AGRN"
                     /gene_synonym="AGRIN; CMS8; CMSPPD"
                     /note="major polyA site"
ORIGIN      
        1 agtcccgtcc ccggcgcggc ccgcgcgctc ctccgccgcc tctcgcctgc gccatggccg
       61 gccggtccca cccgggcccg ctgcggccgc tgctgccgct ccttgtggtg gccgcgtgcg
      121 tcctgcccgg agccggcggg acatgcccgg agcgcgcgct ggagcggcgc gaggaggagg
      181 cgaacgtggt gctcaccggg acggtggagg agatcctcaa cgtggacccg gtgcagcaca
      241 cgtactcctg caaggttcgg gtctggcggt acttgaaggg caaagacctg gtggcccggg
      301 agagcctgct ggacggcggc aacaaggtgg tgatcagcgg ctttggagac cccctcatct
      361 gtgacaacca ggtgtccact ggggacacca ggatcttctt tgtgaaccct gcacccccat
      421 acctgtggcc agcccacaag aacgagctga tgctcaactc cagcctcatg cggatcaccc
      481 tgcggaacct ggaggaggtg gagttctgtg tggaagataa acccgggacc cacttcactc
      541 cagtgcctcc gacgcctcct gatgcgtgcc ggggaatgct gtgcggcttc ggcgccgtgt
      601 gcgagcccaa cgcggagggg ccgggccggg cgtcctgcgt ctgcaagaag agcccgtgcc
      661 ccagcgtggt ggcgcctgtg tgtgggtcgg acgcctccac ctacagcaac gaatgcgagc
      721 tgcagcgggc gcagtgcagc cagcagcgcc gcatccgcct gctcagccgc gggccgtgcg
      781 gctcgcggga cccctgctcc aacgtgacct gcagcttcgg cagcacctgt gcgcgctcgg
      841 ccgacgggct gacggcctcg tgcctgtgcc ccgcgacctg ccgtggcgcc cccgagggga
      901 ccgtctgcgg cagcgacggc gccgactacc ccggcgagtg ccagctcctg cgccgcgcct
      961 gcgcccgcca ggagaatgtc ttcaagaagt tcgacggccc ttgtgacccc tgtcagggcg
     1021 ccctccctga cccgagccgc agctgccgtg tgaacccgcg cacgcggcgc cctgagatgc
     1081 tcctacggcc cgagagctgc cctgcccggc aggcgccagt gtgtggggac gacggagtca
     1141 cctacgaaaa cgactgtgtc atgggccgat cgggggccgc ccggggtctc ctcctgcaga
     1201 aagtgcgctc cggccagtgc cagggtcgag accagtgccc ggagccctgc cggttcaatg
     1261 ccgtgtgcct gtcccgccgt ggccgtcccc gctgctcctg cgaccgcgtc acctgtgacg
     1321 gggcctacag gcccgtgtgt gcccaggacg ggcgcacgta tgacagtgat tgctggcggc
     1381 agcaggctga gtgccggcag cagcgtgcca tccccagcaa gcaccagggc ccgtgtgacc
     1441 aggccccgtc cccatgcctc ggggtgcagt gtgcatttgg ggcgacgtgt gctgtgaaga
     1501 acgggcaggc agcgtgtgaa tgcctgcagg cgtgctcgag cctctacgat cctgtgtgcg
     1561 gcagcgacgg cgtcacatac ggcagcgcgt gcgagctgga ggccacggcc tgtaccctcg
     1621 ggcgggagat ccaggtggcg cgcaaaggac cctgtgaccg ctgcgggcag tgccgctttg
     1681 gagccctgtg cgaggccgag accgggcgct gcgtgtgccc ctctgaatgc gtggctttgg
     1741 cccagcccgt gtgtggctcc gacgggcaca cgtaccccag cgagtgcatg ctgcacgtgc
     1801 acgcctgcac acaccagatc agcctgcacg tggcctcagc tggaccctgt gagacctgtg
     1861 gagatgccgt gtgtgctttt ggggctgtgt gctccgcagg gcagtgtgtg tgtccccggt
     1921 gtgagcaccc cccgcccggc cccgtgtgtg gcagcgacgg tgtcacctac ggcagtgcct
     1981 gcgagctacg ggaagccgcc tgcctccagc agacacagat cgaggaggcc cgggcagggc
     2041 cgtgcgagca ggccgagtgc ggttccggag gctctggctc tggggaggac ggtgactgtg
     2101 agcaggagct gtgccggcag cgcggtggca tctgggacga ggactcggag gacgggccgt
     2161 gtgtctgtga cttcagctgc cagagtgtcc caggcagccc ggtgtgcggc tcagatgggg
     2221 tcacctacag caccgagtgt gagctgaaga aggccaggtg tgagtcacag cgagggctct
     2281 acgtagcggc ccagggagcc tgccgaggcc ccaccttcgc cccgctgccg cctgtggccc
     2341 ccttacactg tgcccagacg ccctacggct gctgccagga caatatcacc gcagcccggg
     2401 gcgtgggcct ggctggctgc cccagtgcct gccagtgcaa cccccatggc tcttacggcg
     2461 gcacctgtga cccagccaca ggccagtgct cctgccgccc aggtgtgggg ggcctcaggt
     2521 gtgaccgctg tgagcctggc ttctggaact ttcgaggcat cgtcaccgat ggccggagtg
     2581 gctgtacacc ctgcagctgt gatccccaag gcgccgtgcg ggatgactgt gagcagatga
     2641 cggggctgtg ctcgtgtaag cccggggtgg ctggacccaa gtgtgggcag tgtccagacg
     2701 gccgtgccct gggccccgcg ggctgtgaag ctgacgcttc tgcgcctgcg acctgtgcgg
     2761 agatgcgctg tgagttcggt gcgcggtgcg tggaggagtc tggctcagcc cactgtgtct
     2821 gcccgatgct cacctgtcca gaggccaacg ctaccaaggt ctgtgggtca gatggagtca
     2881 catacggcaa cgagtgtcag ctgaagacca tcgcctgccg ccagggcctg caaatctcta
     2941 tccagagcct gggcccgtgc caggaggctg ttgctcccag cactcacccg acatctgcct
     3001 ccgtgactgt gaccacccca gggctcctcc tgagccaggc actgccggcc ccccccggcg
     3061 ccctccccct ggctcccagc agtaccgcac acagccagac cacccctccg ccctcatcac
     3121 gacctcggac cactgccagc gtccccagga ccaccgtgtg gcccgtgctg acggtgcccc
     3181 ccacggcacc ctcccctgca cccagcctgg tggcgtccgc ctttggtgaa tctggcagca
     3241 ctgatggaag cagcgatgag gaactgagcg gggaccagga ggccagtggg ggtggctctg
     3301 gggggctcga gcccttggag ggcagcagcg tggccacccc tgggccacct gtcgagaggg
     3361 cttcctgcta caactccgcg ttgggctgct gctctgatgg gaagacgccc tcgctggacg
     3421 cagagggctc caactgcccc gccaccaagg tgttccaggg cgtcctggag ctggagggcg
     3481 tcgagggcca ggagctgttc tacacgcccg agatggctga ccccaagtca gaactgttcg
     3541 gggagacagc caggagcatt gagagcaccc tggacgacct cttccggaat tcagacgtca
     3601 agaaggattt tcggagtgtc cgcttgcggg acctggggcc cggcaaatcc gtccgcgcca
     3661 ttgtggatgt gcactttgac cccaccacag ccttcagggc acccgacgtg gcccgggccc
     3721 tgctccggca gatccaggtg tccaggcgcc ggtccttggg ggtgaggcgg ccgctgcagg
     3781 agcacgtgcg atttatggac tttgactggt ttcctgcgtt tatcacgggg gccacgtcag
     3841 gagccattgc tgcgggagcc acggccagag ccaccactgc atcgcgcctg ccgtcctctg
     3901 ctgtgacccc tcgggccccg caccccagtc acacaagcca gcccgttgcc aagaccacgg
     3961 cagcccccac cacacgtcgg ccccccacca ctgcccccag ccgtgtgccc ggacgtcggc
     4021 ccccggcccc ccagcagcct ccaaagccct gtgactcaca gccctgcttc cacgggggga
     4081 cctgccagga ctgggcattg ggcgggggct tcacctgcag ctgcccggca ggcaggggag
     4141 gcgccgtctg tgagaaggtg cttggcgccc ctgtgccggc cttcgagggc cgctccttcc
     4201 tggccttccc cactctccgc gcctaccaca cgctgcgcct ggcactggaa ttccgggcgc
     4261 tggagcctca ggggctgctg ctgtacaatg gcaacgcccg gggcaaggac ttcctggcat
     4321 tggcgctgct agatggccgc gtgcagctca ggtttgacac aggttcgggg ccggcggtgc
     4381 tgaccagtgc cgtgccggta gagccgggcc agtggcaccg cctggagctg tcccggcact
     4441 ggcgccgggg caccctctcg gtggatggtg agacccctgt tctgggcgag agtcccagtg
     4501 gcaccgacgg cctcaacctg gacacagacc tctttgtggg cggcgtaccc gaggaccagg
     4561 ctgccgtggc gctggagcgg accttcgtgg gcgccggcct gagggggtgc atccgtttgc
     4621 tggacgtcaa caaccagcgc ctggagcttg gcattgggcc gggggctgcc acccgaggct
     4681 ctggcgtggg cgagtgcggg gaccacccct gcctgcccaa cccctgccat ggcggggccc
     4741 catgccagaa cctggaggct ggaaggttcc attgccagtg cccgcccggc cgcgtcggac
     4801 caacctgtgc cgatgagaag agcccctgcc agcccaaccc ctgccatggg gcggcgccct
     4861 gccgtgtgct gcccgagggt ggtgctcagt gcgagtgccc cctggggcgt gagggcacct
     4921 tctgccagac agcctcgggg caggacggct ctgggccctt cctggctgac ttcaacggct
     4981 tctcccacct ggagctgaga ggcctgcaca cctttgcacg ggacctgggg gagaagatgg
     5041 cgctggaggt cgtgttcctg gcacgaggcc ccagcggcct cctgctctac aacgggcaga
     5101 agacggacgg caagggggac ttcgtgtcgc tggcactgcg ggaccgccgc ctggagttcc
     5161 gctacgacct gggcaagggg gcagcggtca tcaggagcag ggagccagtc accctgggag
     5221 cctggaccag ggtctcactg gagcgaaacg gccgcaaggg tgccctgcgt gtgggcgacg
     5281 gcccccgtgt gttgggggag tccccggttc cgcacaccgt cctcaacctg aaggagccgc
     5341 tctacgtagg gggcgctccc gacttcagca agctggcccg tgctgctgcc gtgtcctctg
     5401 gcttcgacgg tgccatccag ctggtctccc tcggaggccg ccagctgctg accccggagc
     5461 acgtgctgcg gcaggtggac gtcacgtcct ttgcaggtca cccctgcacc cgggcctcag
     5521 gccacccctg cctcaatggg gcctcctgcg tcccgaggga ggctgcctat gtgtgcctgt
     5581 gtcccggggg attctcagga ccgcactgcg agaaggggct ggtggagaag tcagcggggg
     5641 acgtggatac cttggccttt gacgggcgga cctttgtcga gtacctcaac gctgtgaccg
     5701 agagcgagaa ggcactgcag agcaaccact ttgaactgag cctgcgcact gaggccacgc
     5761 aggggctggt gctctggagt ggcaaggcca cggagcgggc agactatgtg gcactggcca
     5821 ttgtggacgg gcacctgcaa ctgagctaca acctgggctc ccagcccgtg gtgctgcgtt
     5881 ccaccgtgcc cgtcaacacc aaccgctggt tgcgggtcgt ggcacatagg gagcagaggg
     5941 aaggttccct gcaggtgggc aatgaggccc ctgtgaccgg ctcctccccg ctgggcgcca
     6001 cgcagctgga cactgatgga gccctgtggc ttgggggcct gccggagctg cccgtgggcc
     6061 cagcactgcc caaggcctac ggcacaggct ttgtgggctg cttgcgggac gtggtggtgg
     6121 gccggcaccc gctgcacctg ctggaggacg ccgtcaccaa gccagagctg cggccctgcc
     6181 ccaccccatg agctggcacc agagccccgc gcccgctgta attattttct atttttgtaa
     6241 acttgttgct ttttgatatg attttcttgc ctgagtgttg gccggaggga ctgctggccc
     6301 ggcctccctt ccgtccaggc agccgtgctg cagacagacc tagtgccgag ggatggacag
     6361 gcgaggtggc agcgtggagg gctcggcgtg gatggcagcc tcaggacaca cacccctgcc
     6421 tcaaggtgct gagcccccgc cttgcactgc gcctgcccca cggtgtcccc gccgggaagc
     6481 agccccggct cctgaatcac cctcgctccg tcaggcggga ctcgtgtccc agagaggaag
     6541 gggctgctga ggtctgatgg ggcccttcct ccgggtgacc ccacagggcc tttccaagcc
     6601 cccatttgag ctgctccttc ctgtgtgtgc tctgggccct gcctcggcct cctgcgccaa
     6661 tactgtgact tccaaacaat gttactgctg ggcacagctc tgcgttgctc ccgtgctgcc
     6721 tgcgccagcc ccaggctgct gaggagcaga ggccagacca gggccgatct gggtgtcctg
     6781 accctcagct ggccctgccc agccaccctg gacgtgaccg tatccctctg ccacacccca
     6841 ggccctgcga ggggctatcg agaggagctc actgtgggat ggggttgacc tctgccgcct
     6901 gcctgggtat ctgggcctgg ccatggctgt gttcttcatg tgttgatttt atttgacccc
     6961 tggagtggtg ggtctcatct ttcccatctc gcctgagagc ggctgagggc tgcctcactg
     7021 caaatcctcc ccacagcgtc agtgaaagtc gtccttgtct cagaatgacc aggggccagc
     7081 cagtgtctga ccaaggtcaa ggggcaggtg cagaggtggc agggatggct ccgaagccag
     7141 aaatgcctta aactgcaacg tcccgtccct tccccacccc catcccatcc ccacccccag
     7201 ccccagccca gtcctcctag gagcaggacc cgatgaagcg ggcggcggtg gggctgggtg
     7261 ccgtgttact aactctagta tgtttctgtg tcaatcgctg tgaaataaag tctgaaaact
     7321 ttaaaa
//
