LOCUS       NM_003659               7633 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens alkylglycerone phosphate synthase (AGPS), mRNA.
ACCESSION   NM_003659
VERSION     NM_003659.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Zhang,Y., Huang,Z., Li,K., Xie,G., Feng,Y., Wang,Z., Li,N., Liu,R.,
            Ding,Y., Wang,J., Yang,J. and Jia,Z.
  TITLE     TrkA promotes MDM2-mediated AGPS ubiquitination and degradation to
            trigger prostate cancer progression
  JOURNAL   J Exp Clin Cancer Res 43 (1), 16 (2024)
   PUBMED   38200609
  REMARK    GeneRIF: TrkA promotes MDM2-mediated AGPS ubiquitination and
            degradation to trigger prostate cancer progression.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7633)
  AUTHORS   Zhou,W., Liu,Y., Li,H., Song,Z., Ma,Y. and Zhu,Y.
  TITLE     Mass Spectrometry and Computer Simulation Predict the Interactions
            of AGPS and HNRNPK in Glioma
  JOURNAL   Biomed Res Int 2021, 6181936 (2021)
   PUBMED   34621897
  REMARK    GeneRIF: Mass Spectrometry and Computer Simulation Predict the
            Interactions of AGPS and HNRNPK in Glioma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7633)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 7633)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7633)
  AUTHORS   Zhu,Y., Liu,A., Zhang,X., Qi,L., Zhang,L., Xue,J., Liu,Y. and
            Yang,P.
  TITLE     The effect of benzyl isothiocyanate and its computer-aided design
            derivants targeting alkylglycerone phosphate synthase on the
            inhibition of human glioma U87MG cell line
  JOURNAL   Tumour Biol 36 (5), 3499-3509 (2015)
   PUBMED   25542233
  REMARK    GeneRIF: we optimized the BITC construction targeting
            alkylglycerone phosphate synthase (AGPS) by computer-aided design,
            and the derivants also showed anti-tumor potential in vitro.
REFERENCE   6  (bases 1 to 7633)
  AUTHORS   Wanders,R.J., Dekker,C., Hovarth,V.A., Schutgens,R.B., Tager,J.M.,
            Van Laer,P. and Lecoutere,D.
  TITLE     Human alkyldihydroxyacetonephosphate synthase deficiency: a new
            peroxisomal disorder
  JOURNAL   J Inherit Metab Dis 17 (3), 315-318 (1994)
   PUBMED   7807941
REFERENCE   7  (bases 1 to 7633)
  AUTHORS   Brown,A. and Snyder,F.
  TITLE     Alkyldihydroxyacetonephosphate synthase
  JOURNAL   Methods Enzymol 209, 377-384 (1992)
   PUBMED   1495418
REFERENCE   8  (bases 1 to 7633)
  AUTHORS   Singh,H., Usher,S. and Poulos,A.
  TITLE     Dihydroxyacetone phosphate acyltransferase and
            alkyldihydroxyacetone phosphate synthase activities in rat liver
            subcellular fractions and human skin fibroblasts
  JOURNAL   Arch Biochem Biophys 268 (2), 676-686 (1989)
   PUBMED   2464339
REFERENCE   9  (bases 1 to 7633)
  AUTHORS   Webber,K.O., Datta,N.S. and Hajra,A.K.
  TITLE     Properties of the enzymes catalyzing the biosynthesis of
            lysophosphatidate and its ether analog in cultured fibroblasts from
            Zellweger syndrome patients and normal controls
  JOURNAL   Arch Biochem Biophys 254 (2), 611-620 (1987)
   PUBMED   3646870
REFERENCE   10 (bases 1 to 7633)
  AUTHORS   Schrakamp,G., Roosenboom,C.F., Schutgens,R.B., Wanders,R.J.,
            Heymans,H.S., Tager,J.M. and van den Bosch,H.
  TITLE     Alkyl dihydroxyacetone phosphate synthase in human fibroblasts and
            its deficiency in Zellweger syndrome
  JOURNAL   J Lipid Res 26 (7), 867-873 (1985)
   PUBMED   4031664
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK314259.1, AC073834.6 and
            AA832354.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_003659.3.
            
            Summary: This gene is a member of the FAD-binding
            oxidoreductase/transferase type 4 family. It encodes a protein that
            catalyzes the second step of ether lipid biosynthesis in which
            acyl-dihydroxyacetonephosphate (DHAP) is converted to alkyl-DHAP by
            the addition of a long chain alcohol and the removal of a
            long-chain acid anion. The protein is localized to the inner aspect
            of the peroxisomal membrane and requires FAD as a cofactor.
            Mutations in this gene have been associated with rhizomelic
            chondrodysplasia punctata, type 3 and Zellweger syndrome. [provided
            by RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.69648.1,
                                           SRR1660807.66305.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000264167.11/ ENSP00000264167.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1994              AK314259.1         1-1994
            1995-7426           AC073834.6         31292-36723
            7427-7633           AA832354.1         12-218              c
FEATURES             Location/Qualifiers
     source          1..7633
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q31.2"
     gene            1..7633
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /note="alkylglycerone phosphate synthase"
                     /db_xref="GeneID:8540"
                     /db_xref="HGNC:HGNC:327"
                     /db_xref="MIM:603051"
     exon            1..277
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /inference="alignment:Splign:2.1.0"
     CDS             18..1994
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /EC_number="2.5.1.26"
                     /note="alkyldihydroxyacetonephosphate synthase,
                     peroxisomal; alkyl-DHAP synthase; aging-associated protein
                     5; aging-associated gene 5 protein"
                     /codon_start=1
                     /product="alkyldihydroxyacetonephosphate synthase,
                     peroxisomal precursor"
                     /protein_id="NP_003650.1"
                     /db_xref="CCDS:CCDS2275.1"
                     /db_xref="GeneID:8540"
                     /db_xref="HGNC:HGNC:327"
                     /db_xref="MIM:603051"
                     /translation="MAEAAAAAGGTGLGAGASYGSAADRDRDPDPDRAGRRLRVLSGH
                     LLGRPREALSTNECKARRAASAATAAPTATPAAQESGTIPKKRQEVMKWNGWGYNDSK
                     FIFNKKGQIELTGKRYPLSGMGLPTFKEWIQNTLGVNVEHKTTSKASLNPSDTPPSVV
                     NEDFLHDLKETNISYSQEADDRVFRAHGHCLHEIFLLREGMFERIPDIVLWPTCHDDV
                     VKIVNLACKYNLCIIPIGGGTSVSYGLMCPADETRTIISLDTSQMNRILWVDENNLTA
                     HVEAGITGQELERQLKESGYCTGHEPDSLEFSTVGGWVSTRASGMKKNIYGNIEDLVV
                     HIKMVTPRGIIEKSCQGPRMSTGPDIHHFIMGSEGTLGVITEATIKIRPVPEYQKYGS
                     VAFPNFEQGVACLREIAKQRCAPASIRLMDNKQFQFGHALKPQVSSIFTSFLDGLKKF
                     YITKFKGFDPNQLSVATLLFEGDREKVLQHEKQVYDIAAKFGGLAAGEDNGQRGYLLT
                     YVIAYIRDLALEYYVLGESFETSAPWDRVVDLCRNVKERITRECKEKGVQFAPFSTCR
                     VTQTYDAGACIYFYFAFNYRGISDPLTVFEQTEAAAREEILANGGSLSHHHGVGKLRK
                     QWLKESISDVGFGMLKSVKEYVDPNNIFGNRNLL"
     exon            278..367
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            368..458
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            459..579
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            580..654
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            655..726
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            727..806
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            807..887
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1013
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1014..1122
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1123..1250
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..1302
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1303..1379
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1492
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1493..1562
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1563..1624
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1625..1714
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1715..1814
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1815..1872
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1873..7633
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3212..3217
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /note="hexamer: ATTAAA"
     polyA_site      3236
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /note="major polyA site"
     regulatory      5817..5822
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /note="hexamer: AATAAA"
     polyA_site      5855
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /note="major polyA site"
     regulatory      7613..7618
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /note="hexamer: AATAAA"
     polyA_site      7633
                     /gene="AGPS"
                     /gene_synonym="ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3"
                     /note="major polyA site"
ORIGIN      
        1 agcacaaggc ggtagccatg gcggaggcgg cggctgcagc gggtgggact ggcttgggcg
       61 cgggcgcgag ctacgggtct gcagcggacc gggaccggga cccggacccg gaccgcgccg
      121 ggcggaggct gcgggttctc tctggccatc tgctgggccg gccccgggag gctctgagta
      181 ccaatgagtg caaagcgcgg agagccgcgt cggcggccac ggcagcgccc acggccactc
      241 ccgccgcgca ggagtcgggc accatcccaa agaagcggca agaagttatg aaatggaatg
      301 gatggggata taatgattct aaattcatct tcaataagaa gggccaaatt gaattgactg
      361 ggaaaaggta ccctcttagt ggcatgggtt taccaacatt taaagaatgg atccaaaata
      421 cccttggagt aaatgtggag cataaaacta cctctaaagc atccttaaat cctagtgata
      481 cacctccttc tgttgtaaat gaagattttc ttcatgacct taaagaaact aatatttcat
      541 attcacaaga ggcagatgat cgagtattta gagctcatgg tcattgtctt catgagatat
      601 ttttgctcag ggaaggaatg tttgagcgaa ttcctgatat agttttatgg ccaacatgcc
      661 atgatgatgt agttaagatt gtgaatctag cttgcaaata taatctttgt atcataccaa
      721 ttggtggagg aacaagtgtt tcatatggcc tgatgtgtcc tgcagatgag acaagaacaa
      781 ttatttcttt ggacacttca caaatgaatc gaattctctg ggttgatgag aacaatttga
      841 cagctcatgt agaggctggc ataacaggac aagagttgga aagacagctt aaagaaagtg
      901 gttattgtac aggtcatgaa ccagattccc tggagttcag tactgtagga ggatgggtat
      961 ctactcgcgc atcaggcatg aagaagaata tctatggcaa tatcgaggac ctggtggttc
     1021 atataaaaat ggtaacacct agaggtataa tagaaaaaag ctgtcaagga cctcgtatgt
     1081 caacaggccc tgatatccat cacttcatca tgggatctga aggaactctt ggtgtaataa
     1141 cagaagctac aataaaaatc agaccagtcc ctgaatacca aaagtatggc tcagtagctt
     1201 tccctaattt tgaacaagga gtagcctgtt taagagaaat tgcaaaacag agatgtgctc
     1261 cggcatctat tcgcctcatg gacaacaagc agtttcagtt tggtcatgct cttaaacctc
     1321 aggtttcctc tatttttaca tcatttttgg acggattaaa aaagttttat attacaaagt
     1381 ttaaaggatt tgacccaaat cagctaagtg tagccacatt actgtttgag ggggatcgtg
     1441 agaaggttct tcaacatgaa aaacaagtgt atgatattgc tgcaaaattt ggtgggttgg
     1501 cagctggaga agataatgga cagagaggtt atttgctgac ctatgttatt gcatacattc
     1561 gagacttggc tttggaatac tatgtattag gagaatcttt tgagacttct gctccttggg
     1621 acagggtggt agatctctgt agaaatgtaa aagaaagaat aacaagggaa tgcaaagaga
     1681 agggtgttca gtttgctcct ttttctacat gcagggtgac gcagacttac gatgcaggtg
     1741 cttgtatcta cttctatttt gcctttaact acaggggaat tagtgaccca ctgaccgtat
     1801 ttgaacaaac tgaggcagct gctagagaag aaatccttgc taatggaggg agcctgtcac
     1861 atcaccatgg agtgggcaag ttacggaagc aatggctaaa ggaaagtatc tctgatgtcg
     1921 gctttgggat gctgaagtct gtcaaggaat atgtggaccc caataacatc tttggaaaca
     1981 gaaacctttt ataaatccat tagtaccatt acaaaaaaat gtcaattttt tttttaagtt
     2041 ttcaactgtg gttatactag taatcaaata tatcatggac tatattttgg atacatttgt
     2101 ttctttggtt taaaataagt ttgttttcat tctgtagttt gttttgtttc tacatctatg
     2161 gattgacaga tagtattcct aaatctctct cattgtaggt acatcatttt acattcagcg
     2221 gttgtcattt caaattttag tcaggcagca caaagctgtc aattattcca gaggaagctg
     2281 ctgccagctg ttttgtattg ttgcttcctc ttggggaaca aagacagatt tggtatcatt
     2341 gattgctcag ctagcctctt tttaaaagaa cgtttctggg aatcagatgt ctagggaagg
     2401 attccactga ggagtgatac acgtgtacat tgtttaagag catagtctag tgtgagtagg
     2461 ccattaaggg gaaggatatt catgaaagaa tttaggcaac ctgttattta gaaagccttc
     2521 acgtctgtaa ggtgcttggc ctactttttt ttttaaatta gtatacatgc ataaaacctt
     2581 gtatcatcaa tatacaaatt attctgttag gggacatgtt tttgttctcc atgtttctgt
     2641 ttctattgtg gtatgagttg tatttgtatc actgagataa cctattgtta tattctaatt
     2701 cattgataat atgttttgta agaaaagctg acatccttca ttgcaagtga agaacaggtt
     2761 taccatgaaa tgaatgcctt tattcaactt agagtcctaa tgtttctgat ttcacattct
     2821 tgttgctgaa atgcagaaga aacagctaca gcaagagcag aaggaaaact cttagactta
     2881 atgtctccaa ttgcaagaat tgtttcacta aagaaacagt catcattcaa ctactgaatt
     2941 tgaaagcctc agcagtttca atgacaaaaa tcatttttga ttttttaaaa ttaatcccaa
     3001 tggcatgata aattttcata taaaaagtaa agtttcaatt tagtttttaa taatggttta
     3061 atgccttttt tgaataactg gtttaaccta atttttttta aatgtaatgt attaatgcat
     3121 ataccataat caaaagtttg aaatatcctg tttcttaaat aatgagaaca ttaatcacat
     3181 ttagcaagca tttgtacatt caaacctgga tattaaagtg aaatgattac tttgaatcac
     3241 tgtctgccaa ggttcatgat tcacattttg agatggattc ttttcttaat gcaataccta
     3301 acttttgata attttttaaa actaatattt gatcagataa tgtaagtcaa aatgcagacg
     3361 atcctttaaa aggatgcatt gtctgtgtat gtagcaacag ctccaaagta tttttgggca
     3421 gtttgatacc ttttatatct gaagtagcct taaccagaaa aatggtaatt taaaattgac
     3481 aaaatgatat ttttaagact tcaaaatgat aaataatttt taactattaa aattggcctc
     3541 aaactaataa atctgtaatt aaattagaat taaatatcag tttaacaaca aaatgaaata
     3601 tttatttttt agaagctgcc tctcttcatt ggtcatttat ttttaattat aaaatttcca
     3661 acagatcttt gctttaagcc cttgatattt gacctagttg gacacttgat gaggaagttg
     3721 cctctagaat tataaaaatc tcagctttat aaaatgcatc tgttcacgaa ggtaatttct
     3781 ttaattggtg atcaaaatat aaaattaagg tactaatgtc tcactggaag tatatatata
     3841 tatatatata tatgtatgca tgtgtgtgtg tgtatatata tatatatata tatgtatatg
     3901 tttctgagta atttttaaaa aataaaacag gttaataaga agatccattc ctttgcatgt
     3961 gattgttata acattctttc ccagggagtt caatttcagc aacctcagaa atccatactt
     4021 gatattagga aactgtaaat gtaacacctg aaattattat gttcactatt ctacatgaac
     4081 cttatggaaa ttcatgattt atgtctttgt ttttcttttt ccaagtatat gcaggactcc
     4141 ccaaccttga ttttataatg gtgactgcat tttgtaggtg gaatcatttc taccgaaatc
     4201 attttgaaac acaagaatat tgatcagtac tgtttcactc tgatttagta aatcagaaag
     4261 cctcccccta cccccagccc ccactttcac ataccttctg ggaggtgcct cagtatagac
     4321 ttggtatctg tgaaggactg agttcatgaa tatgcacaga agcacttaaa ccatgcctct
     4381 tatttctata atatctgaaa atggcttcat aaaagttaag tcaggagaac actgtttaca
     4441 tgacacttct taactcattt ttaatgttta tttttaattt caaaaacttg ttgcctattt
     4501 ggttttcagt actgcataac actgactttc ttaaattagt catagttgca ataatttact
     4561 taaatttggt aatgtcacat ttgggttggg gtttcagtgg gaatagtaac ttgccgctag
     4621 tgagaaatgg cattgaatac taaagactca atatgaacag gctttataga cccttagaga
     4681 aactgttgcc ttaatgtttg gtgcaatttc tagtagtgca taagtgaatt tagtcttatg
     4741 ataagtaact ttgagctgtt tttagcttag taagaattgt ggaaaccaca tgtttattgt
     4801 gcccatcatt ttacacaaag aaaagaggat tatattattt cttctaagga agtaggagtt
     4861 ttcttcctaa aagtgcttac atattgtagt actattactt aaaagttatt tatgaagttg
     4921 ggagccttct agtttgcctg tgttgatgta ttttgaaaac aaaattgcaa taaaggatga
     4981 gcttccatcg gtgtgtatgg tataccagat acagatggtt gcaaggaaac cttttccccc
     5041 actagaatag tctattagta actgctaagt cctagcttgc atttttgaaa atgcctttct
     5101 gcatgttagc acccagactt tcctttgtca tattccttta tagctacaac ttaactgtag
     5161 cttatcagag aagctctgta gtcctacact gaagtgtgtt cccttggctg ttcatatggg
     5221 gctgggtttg gttgaacttc cattctgtat aaatgctaga acacagttac tacagattga
     5281 ttcacaaact gcatgtgctg gtgcttgagg cagccaaaat ggaatgctgt ggaaagcata
     5341 tctatatctt aaaaaacact cagacaggag ttaaggacag ctttaataat ttttttgttt
     5401 accacttaat ctttatcaac tttgatttta gttagggctt agctcatggt tgaagaaaag
     5461 tggaaaataa caagtgccca tttaggtttg aagaatccag aatgttttac ctttgacttt
     5521 atgagttata gaagcttggc tttgctctag gcttcatgtt cccaaatgag ccatctgtca
     5581 gtatattcac ttaattctta ctaatttcag tttcctgtaa aggaaacaac ttacatattg
     5641 atgcttaaat ccagaaagtg taaaaggttt tcttaaactg ttaaaatata gtgtctgata
     5701 taaaggatgt aagttactca gctttctgct ccctaagcca aacagtgttt gtcttcagtg
     5761 tgaaatttaa ctgtaaaaac tacttgtaat gtcaagagat tatagatcat attattaata
     5821 aaatggacct gggtctaata ttttttttct tcaaagggag tgtgaagctg tttattcttt
     5881 tatttgcata tgagtttttc ttttagtttt agtttttcat gttcatattt ccactgattt
     5941 gaaaacacaa aatttatttt tataattttg ttgcttgtag cataatcaag tgcaaaatca
     6001 agtgcaacct acttgtgact aaatctcttt ggatttatag ctgaggatat tgttagctag
     6061 gttttgtcaa tccctaatca ttttttaaaa tcaatgctgt attcccagga ggctttgaga
     6121 ctcctatgaa cctgaagcac tcccttttga accttggaag atcctcaaag agtgagcact
     6181 cttgtgctct tctaatcata attaattcct taacaaatca gagtaaggac cactgacata
     6241 tagaaccctc aagccaaaga accatcacag aatttagtaa ctttagttct gtgaacatga
     6301 aaagatttct tttttggttt ccaaaggaga ttatttattt ttccacaagg aaaaacatct
     6361 tgcagagact ctgaattcca aatctgagtg agaaagaaaa ggaaaaaaat ctgtttcatg
     6421 gtggatttct aagcaacata tatacatgtt tttaagacaa ccttttgttg gcagtgtttt
     6481 tcctgaacac catatgcacc ctaggaaaca tgtttttcct ttaatcttgt tcacatatca
     6541 gaaaaggaca tgaaatgctc atactggcat acaagtttct ataagtagtt attttttgag
     6601 ctacttatac tatatcccca aaagctcaca gtgccttgtt gggatttttt ttttccatga
     6661 actagccatt attttaaatg agtgggagag ggtaagtctt tagacagagg tcattcagca
     6721 gttcaagact attcctcttc aagaacatgt caggaattaa gcagttatat gtggaaacac
     6781 tggtaattct ttccacagtg ttgttttgga taatctgttc tagtatattt aactaattgt
     6841 atggtggacc tgtactctga tacacatcga atcagtcaat gggatttgta tgtgcttttc
     6901 cttcctacat tttcattcct actgccccaa cctagtagcc ctaagttgtg gcttggtggg
     6961 tttggcattg agcctcaaga cactcactac attctttctg gatgtttgct cacaggctcc
     7021 ctgaggagca gaacctgtgg agctggggag tgcgggtggt ttctattcct aatattgtgt
     7081 gtcagtcctt tttcgtgtga tggtgccttc agaataacga atatttctgt tctggacaat
     7141 gattaaatct tatgagaaat atatgtaggt tatattaatt cttgtccatt tttgctaagc
     7201 ctgtgaaata ctttggctct gatacaactt aacttgaata atatcttatc ctgagtcttc
     7261 ctccaaggca gtacatattc ctgccaggac tgtagtaatc ctttgggaac tacatgtgag
     7321 taagcacttg tatgtaagcc attatactgc tgtaactaat gcatacaaac aattcttctc
     7381 tttacattta tagtctaaaa ggggatatgc agtgggaagg aaaattcctg agagattcca
     7441 gcatagcttt gtcaattccg actgagccat actgttttcc acaagggctc aacacatagt
     7501 cagtattccg tgctttcagt gtgtgttcag attctcctct tactggtttt cttagactgt
     7561 attgttctat aagcaaatgc ccatgtgtga tttttaagat gtgctgggga aaaataaatc
     7621 aataccacaa cca
//
