LOCUS       NM_020133               7923 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 4
            (AGPAT4), mRNA.
ACCESSION   NM_020133
VERSION     NM_020133.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 7923)
  AUTHORS   Zhang,D., Shi,R., Xiang,W., Kang,X., Tang,B., Li,C., Gao,L.,
            Zhang,X., Zhang,L., Dai,R. and Miao,H.
  TITLE     The Agpat4/LPA axis in colorectal cancer cells regulates antitumor
            responses via p38/p65 signaling in macrophages
  JOURNAL   Signal Transduct Target Ther 5 (1), 24 (2020)
   PUBMED   32296017
  REMARK    GeneRIF: The Agpat4/LPA axis in colorectal cancer cells regulates
            antitumor responses via p38/p65 signaling in macrophages.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7923)
  AUTHORS   Bradley,R.M., Marvyn,P.M., Aristizabal Henao,J.J., Mardian,E.B.,
            George,S., Aucoin,M.G., Stark,K.D. and Duncan,R.E.
  TITLE     Acylglycerophosphate acyltransferase 4 (AGPAT4) is a mitochondrial
            lysophosphatidic acid acyltransferase that regulates brain
            phosphatidylcholine, phosphatidylethanolamine, and
            phosphatidylinositol levels
  JOURNAL   Biochim Biophys Acta 1851 (12), 1566-1576 (2015)
   PUBMED   26417903
REFERENCE   4  (bases 1 to 7923)
  AUTHORS   Schosser,A., Butler,A.W., Uher,R., Ng,M.Y., Cohen-Woods,S.,
            Craddock,N., Owen,M.J., Korszun,A., Gill,M., Rice,J., Hauser,J.,
            Henigsberg,N., Maier,W., Mors,O., Placentino,A., Rietschel,M.,
            Souery,D., Preisig,M., Craig,I.W., Farmer,A.E., Lewis,C.M. and
            McGuffin,P.
  TITLE     Genome-wide association study of co-occurring anxiety in major
            depression
  JOURNAL   World J Biol Psychiatry 14 (8), 611-621 (2013)
   PUBMED   24047446
REFERENCE   5  (bases 1 to 7923)
  AUTHORS   Wu,C., Ma,M.H., Brown,K.R., Geisler,M., Li,L., Tzeng,E., Jia,C.Y.,
            Jurisica,I. and Li,S.S.
  TITLE     Systematic identification of SH3 domain-mediated human
            protein-protein interactions by peptide array target screening
  JOURNAL   Proteomics 7 (11), 1775-1785 (2007)
   PUBMED   17474147
REFERENCE   6  (bases 1 to 7923)
  AUTHORS   Lu,B., Jiang,Y.J., Zhou,Y., Xu,F.Y., Hatch,G.M. and Choy,P.C.
  TITLE     Cloning and characterization of murine 1-acyl-sn-glycerol
            3-phosphate acyltransferases and their regulation by PPARalpha in
            murine heart
  JOURNAL   Biochem J 385 (Pt 2), 469-477 (2005)
   PUBMED   15367102
REFERENCE   7  (bases 1 to 7923)
  AUTHORS   Mungall,A.J., Palmer,S.A., Sims,S.K., Edwards,C.A., Ashurst,J.L.,
            Wilming,L., Jones,M.C., Horton,R., Hunt,S.E., Scott,C.E.,
            Gilbert,J.G., Clamp,M.E., Bethel,G., Milne,S., Ainscough,R.,
            Almeida,J.P., Ambrose,K.D., Andrews,T.D., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Barker,D.J.,
            Barlow,K.F., Bates,K., Beare,D.M., Beasley,H., Beasley,O.,
            Bird,C.P., Blakey,S., Bray-Allen,S., Brook,J., Brown,A.J.,
            Brown,J.Y., Burford,D.C., Burrill,W., Burton,J., Carder,C.,
            Carter,N.P., Chapman,J.C., Clark,S.Y., Clark,G., Clee,C.M.,
            Clegg,S., Cobley,V., Collier,R.E., Collins,J.E., Colman,L.K.,
            Corby,N.R., Coville,G.J., Culley,K.M., Dhami,P., Davies,J.,
            Dunn,M., Earthrowl,M.E., Ellington,A.E., Evans,K.A., Faulkner,L.,
            Francis,M.D., Frankish,A., Frankland,J., French,L., Garner,P.,
            Garnett,J., Ghori,M.J., Gilby,L.M., Gillson,C.J., Glithero,R.J.,
            Grafham,D.V., Grant,M., Gribble,S., Griffiths,C., Griffiths,M.,
            Hall,R., Halls,K.S., Hammond,S., Harley,J.L., Hart,E.A.,
            Heath,P.D., Heathcott,R., Holmes,S.J., Howden,P.J., Howe,K.L.,
            Howell,G.R., Huckle,E., Humphray,S.J., Humphries,M.D., Hunt,A.R.,
            Johnson,C.M., Joy,A.A., Kay,M., Keenan,S.J., Kimberley,A.M.,
            King,A., Laird,G.K., Langford,C., Lawlor,S., Leongamornlert,D.A.,
            Leversha,M., Lloyd,C.R., Lloyd,D.M., Loveland,J.E., Lovell,J.,
            Martin,S., Mashreghi-Mohammadi,M., Maslen,G.L., Matthews,L.,
            McCann,O.T., McLaren,S.J., McLay,K., McMurray,A., Moore,M.J.,
            Mullikin,J.C., Niblett,D., Nickerson,T., Novik,K.L., Oliver,K.,
            Overton-Larty,E.K., Parker,A., Patel,R., Pearce,A.V., Peck,A.I.,
            Phillimore,B., Phillips,S., Plumb,R.W., Porter,K.M., Ramsey,Y.,
            Ranby,S.A., Rice,C.M., Ross,M.T., Searle,S.M., Sehra,H.K.,
            Sheridan,E., Skuce,C.D., Smith,S., Smith,M., Spraggon,L.,
            Squares,S.L., Steward,C.A., Sycamore,N., Tamlyn-Hall,G., Tester,J.,
            Theaker,A.J., Thomas,D.W., Thorpe,A., Tracey,A., Tromans,A.,
            Tubby,B., Wall,M., Wallis,J.M., West,A.P., White,S.S.,
            Whitehead,S.L., Whittaker,H., Wild,A., Willey,D.J., Wilmer,T.E.,
            Wood,J.M., Wray,P.W., Wyatt,J.C., Young,L., Younger,R.M.,
            Bentley,D.R., Coulson,A., Durbin,R., Hubbard,T., Sulston,J.E.,
            Dunham,I., Rogers,J. and Beck,S.
  TITLE     The DNA sequence and analysis of human chromosome 6
  JOURNAL   Nature 425 (6960), 805-811 (2003)
   PUBMED   14574404
REFERENCE   8  (bases 1 to 7923)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   9  (bases 1 to 7923)
  AUTHORS   Leung,D.W.
  TITLE     The structure and functions of human lysophosphatidic acid
            acyltransferases
  JOURNAL   Front Biosci 6, D944-D953 (2001)
   PUBMED   11487472
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   10 (bases 1 to 7923)
  AUTHORS   Dominguez,O., Ashhab,Y., Sabater,L., Belloso,E., Caro,P. and
            Pujol-Borrell,R.
  TITLE     Cloning of ARE-containing genes by AU-motif-directed display
  JOURNAL   Genomics 54 (2), 278-286 (1998)
   PUBMED   9828130
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BQ072907.1, AF156776.1,
            AL109942.13 and AL122123.1.
            
            On Nov 22, 2018 this sequence version replaced NM_020133.2.
            
            Summary: This gene encodes a member of the
            1-acylglycerol-3-phosphate O-acyltransferase family. This integral
            membrane protein converts lysophosphatidic acid to phosphatidic
            acid, the second step in de novo phospholipid biosynthesis.
            [provided by RefSeq, Jul 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.2059716.1,
                                           SRR1660803.159285.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000320285.9/ ENSP00000314036.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-56                BQ072907.1         15-70
            57-1815             AF156776.1         1-1759
            1816-7864           AL109942.13        20526-26574         c
            7865-7865           AL122123.1         5272-5272
            7866-7923           AL109942.13        20467-20524         c
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q26"
     gene            1..7923
                     /gene="AGPAT4"
                     /gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
                     LPLAT4"
                     /note="1-acylglycerol-3-phosphate O-acyltransferase 4"
                     /db_xref="GeneID:56895"
                     /db_xref="HGNC:HGNC:20885"
                     /db_xref="MIM:614795"
     exon            1..124
                     /gene="AGPAT4"
                     /gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
                     LPLAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            125..391
                     /gene="AGPAT4"
                     /gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
                     LPLAT4"
                     /inference="alignment:Splign:2.1.0"
     CDS             214..1350
                     /gene="AGPAT4"
                     /gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
                     LPLAT4"
                     /EC_number="2.3.1.51"
                     /note="1-AGP acyltransferase 4; lysophosphatidic acid
                     acyltransferase-delta (LPAAT-delta); 1-AGPAT 4;
                     lysophosphatidic acid acyltransferase delta;
                     1-acylglycerol-3-phosphate O-acyltransferase 4
                     (lysophosphatidic acid acyltransferase, delta);
                     lysophospholipid acyltransferase 4"
                     /codon_start=1
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase
                     delta"
                     /protein_id="NP_064518.1"
                     /db_xref="CCDS:CCDS5280.1"
                     /db_xref="GeneID:56895"
                     /db_xref="HGNC:HGNC:20885"
                     /db_xref="MIM:614795"
                     /translation="MDLAGLLKSQFLCHLVFCYVFIASGLIINTIQLFTLLLWPINKQ
                     LFRKINCRLSYCISSQLVMLLEWWSGTECTIFTDPRAYLKYGKENAIVVLNHKFEIDF
                     LCGWSLSERFGLLGGSKVLAKKELAYVPIIGWMWYFTEMVFCSRKWEQDRKTVATSLQ
                     HLRDYPEKYFFLIHCEGTRFTEKKHEISMQVARAKGLPRLKHHLLPRTKGFAITVRSL
                     RNVVSAVYDCTLNFRNNENPTLLGVLNGKKYHADLYVRRIPLEDIPEDDDECSAWLHK
                     LYQEKDAFQEEYYRTGTFPETPMVPPRRPWTLVNWLFWASLVLYPFFQFLVSMIRSGS
                     SLTLASFILVFFVASVGVRWMIGVTEIDKGSAYGNSDSKQKLND"
     exon            392..561
                     /gene="AGPAT4"
                     /gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
                     LPLAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            562..723
                     /gene="AGPAT4"
                     /gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
                     LPLAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            724..877
                     /gene="AGPAT4"
                     /gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
                     LPLAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            878..980
                     /gene="AGPAT4"
                     /gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
                     LPLAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1056
                     /gene="AGPAT4"
                     /gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
                     LPLAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1255
                     /gene="AGPAT4"
                     /gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
                     LPLAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1256..7923
                     /gene="AGPAT4"
                     /gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
                     LPLAT4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1795..1800
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AGPAT4"
                     /gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
                     LPLAT4"
                     /note="hexamer: ATTAAA"
     polyA_site      1816
                     /gene="AGPAT4"
                     /gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
                     LPLAT4"
                     /note="major polyA site"
     regulatory      7839..7844
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AGPAT4"
                     /gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
                     LPLAT4"
                     /note="hexamer: AATAAA"
     polyA_site      7860
                     /gene="AGPAT4"
                     /gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
                     LPLAT4"
     regulatory      7891..7896
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AGPAT4"
                     /gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
                     LPLAT4"
                     /note="hexamer: AATAAA"
     polyA_site      7923
                     /gene="AGPAT4"
                     /gene_synonym="1-AGPAT4; dJ473J16.2; LPAAT-delta; LPAAT4;
                     LPLAT4"
ORIGIN      
        1 gctttttctt tccagtgttg gctgacttac agctcttata aactagtggc aatttctgaa
       61 cccagccggc tccatctcag cttctggttt ctaagtccat gtgccaaagg ctgccaggaa
      121 ggagacgcct tcctgagtcc tggatctttc ttccttctgg aaatctttga ctgtgggtag
      181 ttatttattt ctgaataaga gcgtccacgc atcatggacc tcgcgggact gctgaagtct
      241 cagttcctgt gccacctggt cttctgctac gtctttattg cctcagggct aatcatcaac
      301 accattcagc tcttcactct cctcctctgg cccattaaca agcagctctt ccggaagatc
      361 aactgcagac tgtcctattg catctcaagc cagctggtga tgctgctgga gtggtggtcg
      421 ggcacggaat gcaccatctt cacggacccg cgcgcctacc tcaagtatgg gaaggaaaat
      481 gccatcgtgg ttctcaacca caagtttgaa attgactttc tgtgtggctg gagcctgtcc
      541 gaacgctttg ggctgttagg gggctccaag gtcctggcca agaaagagct ggcctatgtc
      601 ccaattatcg gctggatgtg gtacttcacc gagatggtct tctgttcgcg caagtgggag
      661 caggatcgca agacggttgc caccagtttg cagcacctcc gggactaccc cgagaagtat
      721 tttttcctga ttcactgtga gggcacacgg ttcacggaga agaagcatga gatcagcatg
      781 caggtggccc gggccaaggg gctgcctcgc ctcaagcatc acctgttgcc acgaaccaag
      841 ggcttcgcca tcaccgtgag gagcttgaga aatgtagttt cagctgtata tgactgtaca
      901 ctcaatttca gaaataatga aaatccaaca ctgctgggag tcctaaacgg aaagaaatac
      961 catgcagatt tgtatgttag gaggatccca ctggaagaca tccctgaaga cgatgacgag
     1021 tgctcggcct ggctgcacaa gctctaccag gagaaggatg cctttcagga ggagtactac
     1081 aggacgggca ccttcccaga gacgcccatg gtgccccccc ggcggccctg gaccctcgtg
     1141 aactggctgt tttgggcctc gctggtgctc taccctttct tccagttcct ggtcagcatg
     1201 atcaggagcg ggtcttccct gacgctggcc agcttcatcc tcgtcttctt tgtggcctcc
     1261 gtgggagttc gatggatgat tggtgtgacg gaaattgaca agggctctgc ctacggcaac
     1321 tctgacagca agcagaaact gaatgactga ctcagggagg tgtcaccatc cgaagggaac
     1381 cttggggaac tggtggcctc tgcatatcct ccttagtggg acacggtgac aaaggctggg
     1441 tgagcccctg ctgggcacgg cggaagtcac gacctctcca gccagggagt ctggtctcaa
     1501 ggccggatgg ggaggaagat gttttgtaat ctttttttcc ccatgtgctt tagtgggctt
     1561 tggttttctt tttgtgcgag tgtgtgtgag aatggctgtg tggtgagtgt gaactttgtt
     1621 ctgtgatcat agaaagggta ttttaggctg caggggaggg cagggctggg gaccgaaggg
     1681 gacaagttcc cctttcatcc tttggtgctg agttttctgt aacccttggt tgccagagat
     1741 aaagtgaaaa gtgctttagg tgagatgact aaattatgcc tccaagaaaa aaaaattaaa
     1801 gtgcttttct gggtcatctg tggtgtatgt tgacatggac cgctgcccct ccatcctgcc
     1861 cttgcccgtg gctttggtgt ttcagatcct actcacggga ggcagctgcc gcggaggtgt
     1921 gggcagctga gaggggtggg caggccagca cagctcctgg caggagacac aggtccagga
     1981 gcccgtgagc atttgtgaga gcagagatgg caagcacgtg cgtggagacc aacgaagcgt
     2041 gtccctggca cacagcaagg gagagtttcg ctcggtctct gcagtgcagt ctcctggcgt
     2101 aagtcttgaa gatggaccca actccctgaa gaaatggatc ttgaaattga acacaaacat
     2161 catgaacgta cagcctgggc attctgcagt gatttgtgag atgagcttgc atcgactgtt
     2221 cctctcagca gtgacagcca aagtcacccc tgataaaatg cagtttcact ttataaaatg
     2281 cctcgcccct tcccagcatg tagagcttgt ctgcagccct aaaggagcca ggtcctgctc
     2341 tgatgagggt caaatcagag ccgacatccc atgtacaaaa aacccccagg cttctgcctt
     2401 ccttccgggc tcctgtctgc tcccatttta ctccacggaa tagttctgcc ctgtagcatc
     2461 tccagcggcc tttgcagact gctgtcagcc aacaggtgcc aaaaatgcaa aagaagaaga
     2521 aactaaaagc agtgctattt ttgcagaaca aatgctagct attaaatcat cttaggccaa
     2581 gacaccaatg gcacttggac cgataggtat ttggagcatg agttaggaag gatatgtctt
     2641 tctccaggtg cgcagctgaa agttgctgag aaaagctgcc tgtgttgctg tggtaacatg
     2701 agaaggaatg aacagctcta tacaaaaact ggccacacat tataaggcct attctatgca
     2761 gttatgtttt tggctatttg ctcatttagt cattgtttat caaccaaact aggtaagcca
     2821 aggagttaca agacagctct agagaagctc cctctgctta caggacaata atgcattgtt
     2881 tgtttgttga tacaaatgaa ttatttgaat ccacagaagt gaaaaaggga aaaaatcctg
     2941 gctgacttgt gggaagagcg tactgcagag cctagggcct gagttaactc gctggcggcc
     3001 ctgcaggcag gcttgatggg agcgcccagc acatctgccc accctgcaca gggcatgaag
     3061 caccgtgtgc agagatctgt gggcaaaagc ccaggacgtg accttaggca agaggggtcc
     3121 ttggagaagt ggaggtgtgc atggtgtgta gccttgcgtg tctgtgagca cgccctagtg
     3181 gacctgcgcc tggattatcg ggccagtctc tcggaggggt ccgtgatgct gagttacgcc
     3241 attcacctga actctgcagc tcatcggctg taggatttgg gtgagacact tttccttgct
     3301 gactcatttc cccatctgca aaatgggggt gacgatatct acctcttggg gtgtgtcaag
     3361 ggtaaaagag cacactttaa gaatcctaaa gcactaagcc aaggatgaaa gactagaaag
     3421 ctgcctctat gttgctactc tcaaaaatgc tcagaaattt tcttgtcgta ctaaaaatgt
     3481 gttatttttc tcattttcat tcttccattc aacacagaag taatctaagt aagttctaaa
     3541 aacatagatc cgggggccca cagacattca attttcttct gaattggctc tggtatcaca
     3601 caacacacaa gcaagcaaac agcctgcctg ctagggcctt gggttccagg ctggaggaat
     3661 gcaggcagaa atctcttagg aaataactaa gtgagcgcca agttttgaat tgccttagag
     3721 ccgaaagaga atgtggcgtg ctggggagag atctggacct ctttctcatc agtcatttga
     3781 cctgtccacg aagaacaatt ttgttatgca gcctgggaac ctggtttcct tacaccctgc
     3841 gcccttaatg acacagacaa ccacgggtac tcctggacgg aagctcatca cgcctcctca
     3901 aataccttca cccatgttca ggtcacacgg aggtcaccat ctcctcagca cacaacaact
     3961 gctttggttc ccagggttat tttttctacg agttgaaaac ccacggagcc taagccatct
     4021 ttttgtcccc taaggagagc cccatagact aagagctaca cttcctgtct gtctttccca
     4081 cctttccatc tcattctagc ttaaagctta tcacgctact gagagcctgt tctgtagttc
     4141 aaggtggcag agaaaaatac gaaccttgag tgaaatctgg agagcagtgc agggtcatcc
     4201 ccagacgtga cgggccatat cctaggcgac tcctgcagct tatagaagca gggaatttaa
     4261 tggacttctt ccaggaaagc cagcccacgg ggactcttaa aaagacacca accatttgga
     4321 agatgcagct tgaggtctgg gggactggac tgattctgcc accatatagt gaacacgatt
     4381 aatcgggagg aagaagtaag gcactctgtg agttagtcta ccaccctatt gactttgaca
     4441 gagagaaaac caactttttt catatgatca cacagtagta ctggaatatc tcgactgcaa
     4501 tatcctctaa acaaatgcat gcaaagcttt gtaaaagcag ctgatgatta aaaaaatttt
     4561 aaaacattag ggtttttcta atatatccat atgtgggaca gtgatttaaa tgtcattaat
     4621 tgtccagcca caacttgtaa cagtgatgtg aaagacacaa aaaaggcccc actggagtgg
     4681 aggagtggtc tggctggtcc ccaagcaaaa cagaatgggc ttcgcatagg ctttagaact
     4741 tattcataaa tattgaccaa agtgtgaagc aaggtatcca gtgaagattc tctagggtct
     4801 ctacttctgc tcaagagatt taatccagct ttctctaaag cagtggtcca gacttgccac
     4861 atactaggtt aaagataaag tgagaaaaac aaatactttg gtttgcacgt ctcttgaggg
     4921 gccctaggct gtatggagga atatttagag gaatatttgt gagctgccat ttgtgtttaa
     4981 aagaacatga aagcaagacg ggccctaacc cgcctgtgtc tgtagctgac caggaggcca
     5041 ctgcttcctt cccccagagc ttctaggaga gccccccaaa atgcaactct ccaaggtcct
     5101 gtggagtagt cccctcataa tgcctccacc acacctgtgt ctcacgatgc cttaactgac
     5161 gtagctccta agtgatgttc acagagaagg aaatacttag gcaagcccat gtgtaacatg
     5221 ctcacagatg tgctggcttt gccaatgctt tcggactcca ccatgctttc tgattcttct
     5281 ggtcctagtc acgccttggc acggggcaga agcggccgag ctatgctgac aggtgctgct
     5341 gagagccaac agccattcag ccgagaaggc gtttctaggt gagggcatga gagctggcat
     5401 tccaggctgc tgagtgcggc atctgcaggt gcaccacatt gctccacggt gtgttcagaa
     5461 gagaccagca tcttgaatct caggctctgg gctcattctt tgcctctgcc aagggaccat
     5521 tcaggggctt caagccagct gctgaaaatc ccccttttct ggatttttcc ccatctatat
     5581 aaggatatgg tggctgtgtg gacaagaaat tggtttcatc tcagaagggt gcttccctac
     5641 aaagatcttc agaaatttaa ttgaaaacaa ttttttgaac tttttttgtt tggccaggat
     5701 gaatgaagct gaagtcacat cctgaatgtg catgccagac aaagggtaag caagcagtct
     5761 ttacaaatgc caatccctct gcactccaaa accggagccc gtaatgaagg cccaggttcc
     5821 agtcatcaca ggatccccca agccagcaca gtgcctgatg cctccctagg cattagcaca
     5881 tcccagcact ctaattcaca tcctcagcat ccctgcctcc tgcatccagg gcttaggttg
     5941 agtgtttcca gagtgtcaag tacatttaaa cacaaaccaa atactccagg gttttgacct
     6001 gtcacaatgg cttttgaaaa caacagacgt acaatttgag agaaggactc gatggacttc
     6061 ttaggctgag gactaaggac atgttgctgt tcctcttggt ccaggacatt caataccatt
     6121 cacagcattc atcaccaact gacatagtat cgatcacgac attcaactgt ttgaaattac
     6181 tgctattttg gtcaaaaact aagaaatcag aaattgcacc tacatagtgt gtctcctcat
     6241 tggagtccca tctctctgag cacagggatg ggctcctgag ctcacagccc tgccctgccc
     6301 ccccagcagg gcctcaccct catggccctc agtgaatagt gactacatga agggggcact
     6361 tactagaggc ctgagtaatg gaatttcaat tagcttctgc ttcccttttc taaaaaagaa
     6421 ccacctttct taagaataat taggccaaaa taaacttgag taatttgtac agtcaatatt
     6481 ttgaagtttc ggagttacca gtgaaatagc aaagctgttc tccaggaaag gacatccagg
     6541 ttcccaccca ggccttcctt cctcagctcc tgcagcctca tcctagttta atatgaaggt
     6601 tgtgaatggg ttaaaaagca gttcttaagc acctacttag atcttttcag tatctggaaa
     6661 tagcacccag ggctgtgtta gtaaagcttt tcactgggta agagtcatac aatttttaca
     6721 tttaatttgt ttttttaaaa caaggcagtc ttgttttaca tattggaatg actagatatt
     6781 ttttgacatt tctggcacct ctggttctgc tgaattttaa gggaggtgag tggtatgtac
     6841 acaaagttaa gattgctgca taataattcc tttgcatttc caaaataaat tacacaaatg
     6901 tcacttttcc ttttctattc catagtacta gttttagcca gatctttgcc aaacattcta
     6961 ggacattcag aaaagtcatc catggaattt tggaatgcca ttctctctgt atctttgcag
     7021 atcatgagga aggaaagttg atgaactagt acgggatggc tcagacttgt aaagtgtcta
     7081 agtcacaacg ctgcagagcc agcttccggg tagcaatggc atggcctcag ctgtgccaag
     7141 gctacaactg gagcttcctt actcgcatct gaggcaaaca cagcaaggct ggcccaggcc
     7201 gcccacagtc aacagatgtt cacgggagat ggatcagccc ctcttttgct tgttctatca
     7261 aggaaagaga tctaaccatg ggcaagggtg gaacacaccc ggttcaggtc agggaatgag
     7321 ttgctctaag tgaacagtga attcttactt ctcagcctgt atcaattgta cccagggttc
     7381 atgattactg tgacccacta agggatgatc tttgaggaaa cagacctgga gggaggtaat
     7441 ttaaggaggg aaagcgagtg ggttttacag actgctttgt ggcgacattc aggaacagac
     7501 cattcaatgg atgacttttc agtacataaa agcaagcagt aatggccagg agagagcaaa
     7561 gatgccctaa gaatgacatc agactgacca aatgtgcctt ttcaaataag ttatgagaac
     7621 gaatgaattg gaattagagc aaggtattgg acagaaccat gctagtcttt gagagaacgt
     7681 gtggattggg tggatgtttg gaccgaatga atggtaactt tttaatgtga aacagagaac
     7741 tgatatatgg gctgtcatgt caccctaggg tactgtcgct ctgtcatgtg tagcaatctt
     7801 tccaaatttt tgttggtggg ctgaacaaag ataaaactaa taaagtatgt gtggatacca
     7861 ctagacagca cagcttactg gactggatgt aataaaactg actgaaactt taacttggaa
     7921 aaa
//
