LOCUS       NM_020132               6565 bp    mRNA    linear   PRI 11-DEC-2024
DEFINITION  Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 3
            (AGPAT3), transcript variant 1, mRNA.
ACCESSION   NM_020132 NM_031487
VERSION     NM_020132.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Malik,M.A., Saqib,M.A.N., Mientjes,E., Acharya,A., Alam,M.R.,
            Wallaard,I., Schrauwen,I., Bamshad,M.J., Santos-Cortez,R.L.P.,
            Elgersma,Y., Leal,S.M. and Ansar,M.
  TITLE     A loss of function variant in AGPAT3 underlies intellectual
            disability and retinitis pigmentosa (IDRP) syndrome
  JOURNAL   Eur J Hum Genet 31 (12), 1447-1454 (2023)
   PUBMED   37821758
  REMARK    GeneRIF: A loss of function variant in AGPAT3 underlies
            intellectual disability and retinitis pigmentosa (IDRP) syndrome.
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 6565)
  AUTHORS   Lemaitre,R.N., Tanaka,T., Tang,W., Manichaikul,A., Foy,M.,
            Kabagambe,E.K., Nettleton,J.A., King,I.B., Weng,L.C.,
            Bhattacharya,S., Bandinelli,S., Bis,J.C., Rich,S.S., Jacobs,D.R.
            Jr., Cherubini,A., McKnight,B., Liang,S., Gu,X., Rice,K.,
            Laurie,C.C., Lumley,T., Browning,B.L., Psaty,B.M., Chen,Y.D.,
            Friedlander,Y., Djousse,L., Wu,J.H., Siscovick,D.S.,
            Uitterlinden,A.G., Arnett,D.K., Ferrucci,L., Fornage,M., Tsai,M.Y.,
            Mozaffarian,D. and Steffen,L.M.
  TITLE     Genetic loci associated with plasma phospholipid n-3 fatty acids: a
            meta-analysis of genome-wide association studies from the CHARGE
            Consortium
  JOURNAL   PLoS Genet 7 (7), e1002193 (2011)
   PUBMED   21829377
REFERENCE   4  (bases 1 to 6565)
  AUTHORS   Prasad,S.S., Garg,A. and Agarwal,A.K.
  TITLE     Enzymatic activities of the human AGPAT isoform 3 and isoform 5:
            localization of AGPAT5 to mitochondria
  JOURNAL   J Lipid Res 52 (3), 451-462 (2011)
   PUBMED   21173190
  REMARK    GeneRIF: enzymatic properties, tissue distribution, and subcellular
            localization of human AGPAT3 and AGPAT5
REFERENCE   5  (bases 1 to 6565)
  AUTHORS   Schmidt,J.A., Yvone,G.M. and Brown,W.J.
  TITLE     Membrane topology of human AGPAT3 (LPAAT3)
  JOURNAL   Biochem Biophys Res Commun 397 (4), 661-667 (2010)
   PUBMED   20537980
  REMARK    GeneRIF: The data is consistent with a structural arrangement in
            which motif I is located in the cytoplasm and motif II is in the
            endoplasmic reticulum and Golgi lumen, suggesting a different model
            for AGPAT3/LPAAT3's enzymatic mechanism.
REFERENCE   6  (bases 1 to 6565)
  AUTHORS   Agarwal,A.K., Barnes,R.I. and Garg,A.
  TITLE     Functional characterization of human 1-acylglycerol-3-phosphate
            acyltransferase isoform 8: cloning, tissue distribution, gene
            structure, and enzymatic activity
  JOURNAL   Arch Biochem Biophys 449 (1-2), 64-76 (2006)
   PUBMED   16620771
  REMARK    GeneRIF: Of the two well conserved acyltransferase motifs, NHX(4)D
            is present in AGPAT8, whereas arginine in the EGTR motif is
            substituted by aspartate.
REFERENCE   7  (bases 1 to 6565)
  AUTHORS   Lu,B., Jiang,Y.J., Zhou,Y., Xu,F.Y., Hatch,G.M. and Choy,P.C.
  TITLE     Cloning and characterization of murine 1-acyl-sn-glycerol
            3-phosphate acyltransferases and their regulation by PPARalpha in
            murine heart
  JOURNAL   Biochem J 385 (Pt 2), 469-477 (2005)
   PUBMED   15367102
REFERENCE   8  (bases 1 to 6565)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   9  (bases 1 to 6565)
  AUTHORS   Wistow,G., Bernstein,S.L., Ray,S., Wyatt,M.K., Behal,A.,
            Touchman,J.W., Bouffard,G., Smith,D. and Peterson,K.
  TITLE     Expressed sequence tag analysis of adult human iris for the NEIBank
            Project: steroid-response factors and similarities with retinal
            pigment epithelium
  JOURNAL   Mol Vis 8, 185-195 (2002)
   PUBMED   12107412
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 6565)
  AUTHORS   Leung,D.W.
  TITLE     The structure and functions of human lysophosphatidic acid
            acyltransferases
  JOURNAL   Front Biosci 6, D944-D953 (2001)
   PUBMED   11487472
  REMARK    Review article
            Publication Status: Online-Only
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP001053.1, CD672915.1,
            BC063552.1, AP001054.1 and AB001523.1.
            
            On Nov 22, 2018 this sequence version replaced NM_020132.4.
            
            Summary: The protein encoded by this gene is an acyltransferase
            that converts lysophosphatidic acid into phosphatidic acid, which
            is the second step in the de novo phospholipid biosynthetic
            pathway. The encoded protein may be an integral membrane protein.
            Two transcript variants encoding the same protein have been found
            for this gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 both encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.155937.1,
                                           SRR1803616.76462.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000291572.13/ ENSP00000291572.8
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-12                AP001053.1         107069-107080
            13-538              CD672915.1         1-526
            539-2076            BC063552.1         797-2334
            2077-5889           AP001054.1         89037-92849
            5890-6565           AB001523.1         1-676
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="21"
                     /map="21q22.3"
     gene            1..6565
                     /gene="AGPAT3"
                     /gene_synonym="1-AGPAT 3; LPAAT-GAMMA1; LPAAT3; LPLAT3"
                     /note="1-acylglycerol-3-phosphate O-acyltransferase 3"
                     /db_xref="GeneID:56894"
                     /db_xref="HGNC:HGNC:326"
                     /db_xref="MIM:614794"
     exon            1..123
                     /gene="AGPAT3"
                     /gene_synonym="1-AGPAT 3; LPAAT-GAMMA1; LPAAT3; LPLAT3"
                     /inference="alignment:Splign:2.1.0"
     exon            124..186
                     /gene="AGPAT3"
                     /gene_synonym="1-AGPAT 3; LPAAT-GAMMA1; LPAAT3; LPLAT3"
                     /inference="alignment:Splign:2.1.0"
     exon            187..412
                     /gene="AGPAT3"
                     /gene_synonym="1-AGPAT 3; LPAAT-GAMMA1; LPAAT3; LPLAT3"
                     /inference="alignment:Splign:2.1.0"
     CDS             235..1365
                     /gene="AGPAT3"
                     /gene_synonym="1-AGPAT 3; LPAAT-GAMMA1; LPAAT3; LPLAT3"
                     /EC_number="2.3.1.51"
                     /note="isoform 1 is encoded by transcript variant 1;
                     1-acyl-sn-glycerol-3-phosphate acyltransferase gamma;
                     1-AGP acyltransferase 3; lysophosphatidic acid
                     acyltransferase-gamma1; lysophosphatidic acid
                     acyltransferase gamma; lysophospholipid acyltransferase 3"
                     /codon_start=1
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase
                     gamma isoform 1"
                     /protein_id="NP_064517.1"
                     /db_xref="CCDS:CCDS13703.1"
                     /db_xref="GeneID:56894"
                     /db_xref="HGNC:HGNC:326"
                     /db_xref="MIM:614794"
                     /translation="MGLLAFLKTQFVLHLLVGFVFVVSGLVINFVQLCTLALWPVSKQ
                     LYRRLNCRLAYSLWSQLVMLLEWWSCTECTLFTDQATVERFGKEHAVIILNHNFEIDF
                     LCGWTMCERFGVLGSSKVLAKKELLYVPLIGWTWYFLEIVFCKRKWEEDRDTVVEGLR
                     RLSDYPEYMWFLLYCEGTRFTETKHRVSMEVAAAKGLPVLKYHLLPRTKGFTTAVKCL
                     RGTVAAVYDVTLNFRGNKNPSLLGILYGKKYEADMCVRRFPLEDIPLDEKEAAQWLHK
                     LYQEKDALQEIYNQKGMFPGEQFKPARRPWTLLNFLSWATILLSPLFSFVLGVFASGS
                     PLLILTFLGFVGAASFGVRRLIGVTEIEKGSSYGNQEFKKKE"
     exon            413..582
                     /gene="AGPAT3"
                     /gene_synonym="1-AGPAT 3; LPAAT-GAMMA1; LPAAT3; LPLAT3"
                     /inference="alignment:Splign:2.1.0"
     exon            583..744
                     /gene="AGPAT3"
                     /gene_synonym="1-AGPAT 3; LPAAT-GAMMA1; LPAAT3; LPLAT3"
                     /inference="alignment:Splign:2.1.0"
     exon            745..898
                     /gene="AGPAT3"
                     /gene_synonym="1-AGPAT 3; LPAAT-GAMMA1; LPAAT3; LPLAT3"
                     /inference="alignment:Splign:2.1.0"
     exon            899..1001
                     /gene="AGPAT3"
                     /gene_synonym="1-AGPAT 3; LPAAT-GAMMA1; LPAAT3; LPLAT3"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1077
                     /gene="AGPAT3"
                     /gene_synonym="1-AGPAT 3; LPAAT-GAMMA1; LPAAT3; LPLAT3"
                     /inference="alignment:Splign:2.1.0"
     exon            1078..1276
                     /gene="AGPAT3"
                     /gene_synonym="1-AGPAT 3; LPAAT-GAMMA1; LPAAT3; LPLAT3"
                     /inference="alignment:Splign:2.1.0"
     exon            1277..6565
                     /gene="AGPAT3"
                     /gene_synonym="1-AGPAT 3; LPAAT-GAMMA1; LPAAT3; LPLAT3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3627..3632
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AGPAT3"
                     /gene_synonym="1-AGPAT 3; LPAAT-GAMMA1; LPAAT3; LPLAT3"
                     /note="hexamer: AATAAA"
     polyA_site      3646
                     /gene="AGPAT3"
                     /gene_synonym="1-AGPAT 3; LPAAT-GAMMA1; LPAAT3; LPLAT3"
                     /note="major polyA site"
     regulatory      5481..5486
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AGPAT3"
                     /gene_synonym="1-AGPAT 3; LPAAT-GAMMA1; LPAAT3; LPLAT3"
                     /note="hexamer: ATTAAA"
     polyA_site      5509
                     /gene="AGPAT3"
                     /gene_synonym="1-AGPAT 3; LPAAT-GAMMA1; LPAAT3; LPLAT3"
                     /note="major polyA site"
     regulatory      6097..6102
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AGPAT3"
                     /gene_synonym="1-AGPAT 3; LPAAT-GAMMA1; LPAAT3; LPLAT3"
                     /note="hexamer: AATATA"
     polyA_site      6113
                     /gene="AGPAT3"
                     /gene_synonym="1-AGPAT 3; LPAAT-GAMMA1; LPAAT3; LPLAT3"
     regulatory      6540..6545
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AGPAT3"
                     /gene_synonym="1-AGPAT 3; LPAAT-GAMMA1; LPAAT3; LPLAT3"
                     /note="hexamer: AACAAA"
     polyA_site      6565
                     /gene="AGPAT3"
                     /gene_synonym="1-AGPAT 3; LPAAT-GAMMA1; LPAAT3; LPLAT3"
ORIGIN      
        1 actcgctgag gccccgacgc agggccgggc cgggcccagg gccgaggagc gcggcggcca
       61 gagcggggcc gcggaggcga cgccggggac gcccgcgcga cgagcaggtg gcggcggctg
      121 caggcttgtc cagccggaag ccctgagggc agctgttccc actggctctg ctgaccttgt
      181 gccttggacg gctgtcctca gcgaggggcc gtgcacccgc tcctgagcag cgccatgggc
      241 ctgctggcct tcctgaagac ccagttcgtg ctgcacctgc tggtcggctt tgtcttcgtg
      301 gtgagtggtc tggtcatcaa cttcgtccag ctgtgcacgc tggcgctctg gccggtcagc
      361 aagcagctct accgccgcct caactgccgc ctcgcctact cactctggag ccaactggtc
      421 atgctgctgg agtggtggtc ctgcacggag tgtacactgt tcacggacca ggccacggta
      481 gagcgctttg ggaaggagca cgcagtcatc atcctcaacc acaacttcga gatcgacttc
      541 ctctgtgggt ggaccatgtg tgagcgcttc ggagtgctgg ggagctccaa ggtcctcgct
      601 aagaaggagc tgctctacgt gcccctcatc ggctggacgt ggtactttct ggagattgtg
      661 ttctgcaagc ggaagtggga ggaggaccgg gacaccgtgg tcgaagggct gaggcgcctg
      721 tcggactacc ccgagtacat gtggtttctc ctgtactgcg aggggacgcg cttcacggag
      781 accaagcacc gcgttagcat ggaggtggcg gctgctaagg ggcttcctgt cctcaagtac
      841 cacctgctgc cgcggaccaa gggcttcacc accgcagtca agtgcctccg ggggacagtc
      901 gcagctgtct atgatgtaac cctgaacttc agaggaaaca agaacccgtc cctgctgggg
      961 atcctctacg ggaagaagta cgaggcggac atgtgcgtga ggagatttcc tctggaagac
     1021 atcccgctgg atgaaaagga agcagctcag tggcttcata aactgtacca ggagaaggac
     1081 gcgctccagg agatatataa tcagaagggc atgtttccag gggagcagtt taagcctgcc
     1141 cggaggccgt ggaccctcct gaacttcctg tcctgggcca ccattctcct gtctcccctc
     1201 ttcagttttg tcttgggcgt ctttgccagc ggatcacctc tcctgatcct gactttcttg
     1261 gggtttgtgg gagcagcttc ctttggagtt cgcagactga taggagtaac tgagatagaa
     1321 aaaggctcca gctacggaaa ccaagagttt aagaaaaagg aataattaat ggctgtgact
     1381 gaacacacgc ggccctgacg gtggtatcca gttaactcaa aaccaacaca cagagtgcag
     1441 gaaaagacaa ttagaaacta tttttcttat taactggtga ctaatattaa caaaacttga
     1501 gccaagagta aagaattcag aaggcctgtc aggtgaagtc ttcagcctcc cacagcgcag
     1561 ggtcccagca tctccacgcg cgcccgtggg aggtgggtcc ggccggagag gcctcccgcg
     1621 gacgccgtct ctccagaact ccgcttccaa gagggagcct ttggctgctt tctctcctta
     1681 aacttagatc aaattttttg gtttttaatc agttatcttg ggaacttaac ctggcccctc
     1741 acctcttctg caccccccgc ccccgaaact gtctcgtaat gaatttctgc tgtcctcctg
     1801 ggagtggacg gccgggtccc gtcccccggg agcatcgctc ggctcagcac cttggctccc
     1861 agtgggggcc ccgtggaggg cgcccgtagt gataagcaca ccggcacgaa cgtcaggtcc
     1921 attcctcgaa gtcggagccc tcactctgcc ctgtcctggg gctggctgag ggcgaacgcc
     1981 ccacctcact ttctagagcc ctgtctgtcc tagctcctat ctgaccttgt gtgtaaatac
     2041 gtacatctgt ttttaaagtg gatgggcccc tgagaactca gtgaaatgca gagttctcca
     2101 tgcacctaaa gctcctttgt cgctctcatg gctgtcagat cctggtccct ccacactggg
     2161 tgctggggag ggaggaccct cggggctacc gcgcgccccc ccatcccaca gatcaggagc
     2221 caaggaggga gaacagggca gcctgtggga ctctaggatg cttcagaaga agcgacggca
     2281 ccgtcaaccc tctgtttttt aaaggtggtt ggagactgtt aacactgagc tcattgactt
     2341 ctagagattt tatttttact ggttgatctc ttggtggttt tcaacttcct gctggaaact
     2401 agaggtgggg caccccccac cccccagcct cgcactgtgt ccttggggaa ggcccgcccc
     2461 catcctggcc ggtgtcactg tggcccggcc acccctgagc gcccagctcc ctacctcctg
     2521 gacgtctctg agagtccagg cagagcagag ggcagcgctc ggccggtcat gctggctccc
     2581 ttggccttgc agcgagcccc tggcccacgc cgagcgaggg atgcttctcc ctacagcatg
     2641 tccactcccc cggcatggcc aggtggggcc cctggggcaa tggcagtggt agaacgctca
     2701 acttggttgc ggtaccatca gcccacctgc atttggcttt cgacttgctt gttctaagtc
     2761 acagcgccct catcttttta gcaaggtaaa aaaaccaaaa tgggtgttat ctctgatatc
     2821 ttgaaaccag cgttctgaat agaggtaggt tgagttttct aggggaaaac aaatggagaa
     2881 aagaggcatg aagaaaagta aaccgagaac ataattaggc atcgggccta agtgtcctgg
     2941 ggagattgga ggggacggca gcgttctgca tgatggaggc gctgccgggc cccgggtctg
     3001 tgggggccgt gctctcaggg cgtgtgcggg acgccacctg tgcacacctg ctcagagcac
     3061 ggctcctcgc aggggtgaag gggcagacca acgaaaccag atgagaccaa cgacaccatg
     3121 cgagacacgc ttgcagacac tgttgttttg gaaatgtgct tccctccatc tgaaatctca
     3181 tccctccacc cgcccactcg ggcagctgtg ccgtgggcag ggcatgcgct cccctggctg
     3241 agcaccccag agattctcct gcaccttcct catgccgcac gctgctcatc cgtctccatg
     3301 tgtgtttaga tccatgccat tcactgactc actaacacct gcaaaatctt taaggaaaaa
     3361 agctgaaggg tacgaccatg cacatatgtg acctggaaaa tgcaaattta gatcttttat
     3421 gatttaatta ttattgtttc ccatagaagt tccctccctt tgaaattaat atataatgta
     3481 taaattctgc actgagccat ggcggagctg ggcagcccct aggttagagt ggagacggag
     3541 gcccaggcgc aggggtcaca cctcatctgg tttccttccc atctcacagc ttagcttgtg
     3601 cttctcaaca ccaagtcttt aagagcaata aaaactacac catgaatgtt tgaatttttt
     3661 ttttttgggg gggggagggt ggattttgct tttcatccag aaggaaaagg ggaggagagc
     3721 tcctttacat tttttaaatt aaattcataa atcccagaac agtctttttt ttttcttttt
     3781 cctttacacc ctacttctga gcttaattca gttgatgtta tgtccaaatt caggctgagt
     3841 gctcaggctg aagcaactct gtagcccaca gtccgtgctg gccactgtcg gggtgaggca
     3901 cattctaggc ctggcatcgc tgatgccctc tgcacccagt ccttgagcca ggccgaggac
     3961 aggaagggca ttgctggcct gtagctcctg ttacccaccc agagccaggc gccacactgg
     4021 aggctcccag gcgggagggc ccaggcccac ccacgggcgt ctggccggtc aagctcccag
     4081 cctgggccgt ggtctcctgg ggacgccgct ggcctcccag cttaggccca ggtgccaggt
     4141 gtcatggggt ctcctgccca tcttcccaag gatgccattg ctgtcttcat cgtcttcccc
     4201 cgtgtgagac actggttgtc tggtactcgg cgacagtgta ccagacgcgc accctatgtg
     4261 aggtgcttta aggtccctgt cctcaggaag cgcagtctgg tggaagagat gagcgctgaa
     4321 caaatcacta aataacacaa aacaacaatg taagcagcac tttcttgtgt aaaaaaaaaa
     4381 aaaaagcacg tcctgtcgat gaattttgag tctctctgcc ttgcctgtcc cgactccctt
     4441 tcgcgcaccg tggcatgaga atctttcctc acgctgttcg ttgcggtatt gctgagcatt
     4501 gatgttgatt tgaaggaaaa gccgtggttg gacccagctg tgtgtttcac tcacgtgcaa
     4561 ttgagtagct gttgccaggc tgtccatgcc tgttgtgact accggttggg gtcagatttg
     4621 gggtgaagaa tgagtaagtt tctgcatgct ttgtctctac ctgagacact tctacaacaa
     4681 gccaagtata atctctccag ccctgcagat tttcacctga cttgttcagc cccatgcgta
     4741 gactcccgct gcaggcctct ggcctgtggc tcactgcatg cagcccctgg cgtgcaatac
     4801 tagtgctcca cggcgcgatg tgcttctagc ccttgcactg cacctaggct cagggttcaa
     4861 acggccagcc cgaaaagcct gcctgccttc tttctggaaa cagcacgtcc ccggccgtgt
     4921 gcctgcccct ttctctactg agctagtccc caaaccaaag gcaagccccc tcgggcctcg
     4981 ggggatgggg ccggccacac ccctgactcc gccctggctc tgccccatac ccctgccgtg
     5041 gggccgacct gggggatgca gacatccggc tccgtattcc tgcctatcgg ggccaggatg
     5101 caaaaacaat ttttgcgtaa aagatgtcac actgatctgc tggagtgggg tggacacatg
     5161 aattcagttt tatcatgaac actcgccact ggctgcttgt taattcaggg ataatggtgg
     5221 cattcttaca aactgctcgg gaaatagaat gacgggaaca cttttaggga gcccaggaag
     5281 ttaccaggga cattggtgtc gccggcccag gcaacagcag cgtacgcttt tcaaagatca
     5341 ttgagttgtc ttagaatttg aagctgtgta atgacaatgt cacctggagt tcgtctccat
     5401 ttcttaactt tttgttgcac aagtatttgg acagaagtcg aactgtgaat gagatactga
     5461 aatgcactaa attgtattac attaaactgg agttacttga tacaatgaag aaatgcatct
     5521 gatctacttg tatttattgt ctcagaattg tacgttgtga caatcaaatg ttacttgcct
     5581 aaatgccagg aaattcaact ggccttttaa tcagcaagat cgccagcaag tactgtaact
     5641 gtaaaggaaa atctctctct ctggagaacc cacacaaacc attgacctga gtgcgcatga
     5701 cagccactgg cttctttttc tatgattgaa aatctgccat cgctgactgt tggccagttt
     5761 caaagggacc cattgtatac agggtgcaaa tgtattatac ggatgtttcc ttttgtacac
     5821 ttcattttta caagttttgc tactcacaag ctttatgtag tggaggatag aggtattttt
     5881 ggtctttaga agcttgtcgg ggtgagggct gctaacttac acttcagagg cctgtgtccc
     5941 aaaggcctgg ctgcgtttgc cgtgctgtgc gaggacctgt gtacacaggc aggtgtgcgc
     6001 ctgcccgagc gcgagtagct cttgtgtagt ggtgaaatgc tgcaggcatc tgtttattaa
     6061 aattgctgct gtgaaagaca gggataaaat atccacaata taagagaaag cgactccgtg
     6121 cctccttctg tttcttcgtt tccagtctgc atagatgtct ccaaggggaa accagggcca
     6181 ccaggcactc ctgtcccatg gggccaccca ccttcagagc ccactcccac gggctctgtt
     6241 tccttggagg ccatcctgcc gatggcactt ttcaaggccc tcccttccct acgagttgct
     6301 ttctgggagg ggaaattgaa aaggagagcg gtcacctggc aaaaacacag gggaaatcag
     6361 ccagtccaca aaaatacgca aaatgacctg atgatgtcca aaaaagggtt ttaaaagcga
     6421 tcaaggacaa aaggttttcc taagtggcat gtatacatcc aaaactactc tttttttgta
     6481 aaaacattca tctgtggaga aaaccaagga acccctttct cttctttatt aaaaagcaaa
     6541 acaaaaactc ttgttagaac caaaa
//
