LOCUS       NM_017629               7272 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens argonaute RISC component 4 (AGO4), transcript variant
            1, mRNA.
ACCESSION   NM_017629
VERSION     NM_017629.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Wang,L., Li,D., Su,X., Zhao,Y., Huang,A., Li,H., Li,J., Xia,W.,
            Jia,T., Zhang,H., Dong,J., Liu,X. and Shao,N.
  TITLE     AGO4 suppresses tumor growth by modulating autophagy and apoptosis
            via enhancing TRIM21-mediated ubiquitination of GRP78 in a
            p53-independent manner
  JOURNAL   Oncogene 42 (1), 62-77 (2023)
   PUBMED   36371565
  REMARK    GeneRIF: AGO4 suppresses tumor growth by modulating autophagy and
            apoptosis via enhancing TRIM21-mediated ubiquitination of GRP78 in
            a p53-independent manner.
REFERENCE   2  (bases 1 to 7272)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 7272)
  AUTHORS   Choi,E.J., Ren,J., Zhang,K., Wu,W., Lee,Y.S., Lee,I. and Bao,X.
  TITLE     The Importance of AGO 1 and 4 in Post-Transcriptional Gene
            Regulatory Function of tRF5-GluCTC, an Respiratory Syncytial
            Virus-Induced tRNA-Derived RNA Fragment
  JOURNAL   Int J Mol Sci 21 (22), 8766 (2020)
   PUBMED   33233493
  REMARK    GeneRIF: The Importance of AGO 1 and 4 in Post-Transcriptional Gene
            Regulatory Function of tRF5-GluCTC, an Respiratory Syncytial
            Virus-Induced tRNA-Derived RNA Fragment.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7272)
  AUTHORS   Hauptmann,J., Kater,L., Loffler,P., Merkl,R. and Meister,G.
  TITLE     Generation of catalytic human Ago4 identifies structural elements
            important for RNA cleavage
  JOURNAL   RNA 20 (10), 1532-1538 (2014)
   PUBMED   25114291
REFERENCE   5  (bases 1 to 7272)
  AUTHORS   Schurmann,N., Trabuco,L.G., Bender,C., Russell,R.B. and Grimm,D.
  TITLE     Molecular dissection of human Argonaute proteins by DNA shuffling
  JOURNAL   Nat Struct Mol Biol 20 (7), 818-826 (2013)
   PUBMED   23748378
  REMARK    GeneRIF: Chimeras with Ago4 N-terminal domains were defective in
            RISC activation & sometimes also in RNA loading. This highlights
            the pleiotropic role of the Ago N domain & emphasizes the unique
            position of human Ago4 within the Ago protein family.
REFERENCE   6  (bases 1 to 7272)
  AUTHORS   Pillai,R.S., Artus,C.G. and Filipowicz,W.
  TITLE     Tethering of human Ago proteins to mRNA mimics the miRNA-mediated
            repression of protein synthesis
  JOURNAL   RNA 10 (10), 1518-1525 (2004)
   PUBMED   15337849
REFERENCE   7  (bases 1 to 7272)
  AUTHORS   Meister,G., Landthaler,M., Patkaniowska,A., Dorsett,Y., Teng,G. and
            Tuschl,T.
  TITLE     Human Argonaute2 mediates RNA cleavage targeted by miRNAs and
            siRNAs
  JOURNAL   Mol Cell 15 (2), 185-197 (2004)
   PUBMED   15260970
REFERENCE   8  (bases 1 to 7272)
  AUTHORS   Sasaki,T., Shiohama,A., Minoshima,S. and Shimizu,N.
  TITLE     Identification of eight members of the Argonaute family in the
            human genome
  JOURNAL   Genomics 82 (3), 323-330 (2003)
   PUBMED   12906857
REFERENCE   9  (bases 1 to 7272)
  AUTHORS   Doi,N., Zenno,S., Ueda,R., Ohki-Hamazaki,H., Ui-Tei,K. and Saigo,K.
  TITLE     Short-interfering-RNA-mediated gene silencing in mammalian cells
            requires Dicer and eIF2C translation initiation factors
  JOURNAL   Curr Biol 13 (1), 41-46 (2003)
   PUBMED   12526743
REFERENCE   10 (bases 1 to 7272)
  AUTHORS   Carmell,M.A., Xuan,Z., Zhang,M.Q. and Hannon,G.J.
  TITLE     The Argonaute family: tentacles that reach into RNAi, developmental
            control, stem cell maintenance, and tumorigenesis
  JOURNAL   Genes Dev 16 (21), 2733-2742 (2002)
   PUBMED   12414724
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL354864.16, AB046787.1,
            AL359186.15 and CA419947.1.
            
            On Nov 22, 2018 this sequence version replaced NM_017629.3.
            
            Summary: This gene encodes a member of the Argonaute family of
            proteins which contain PAZ and PIWI domains and play an integral
            role in RNA interference and short-interfering-RNA-mediated gene
            silencing. This gene is located on chromosome 1 in a cluster of
            related family members. [provided by RefSeq, Mar 2017].
            
            Sequence Note: Sequence Note: This RefSeq record was created from
            transcript and genomic sequence data to make the sequence
            consistent with the reference genome assembly. The genomic
            coordinates used for the transcript record were based on transcript
            alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC152450.1, AB046787.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373210.4/ ENSP00000362306.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-211               AL354864.16        6024-6234           c
            212-6201            AB046787.1         1-5990
            6202-7057           AL359186.15        44571-45426
            7058-7272           CA419947.1         9-223               c
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p34.3"
     gene            1..7272
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /note="argonaute RISC component 4"
                     /db_xref="GeneID:192670"
                     /db_xref="HGNC:HGNC:18424"
                     /db_xref="MIM:607356"
     exon            1..420
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     CDS             402..2987
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /note="eukaryotic translation initiation factor 2C, 4;
                     argonaute 4, RISC catalytic component; argonaute RISC
                     catalytic component 4"
                     /codon_start=1
                     /product="protein argonaute-4"
                     /protein_id="NP_060099.2"
                     /db_xref="CCDS:CCDS397.1"
                     /db_xref="GeneID:192670"
                     /db_xref="HGNC:HGNC:18424"
                     /db_xref="MIM:607356"
                     /translation="MEALGPGPPASLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDV
                     YHYDVDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDR
                     VDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGHLNEVPDDSVQALDVITRHLP
                     SMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQ
                     PIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTR
                     RPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVC
                     NIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIV
                     VHNEMTELTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAP
                     QKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQ
                     LIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGI
                     NNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQ
                     TSRQEISQELLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVA
                     WPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDS
                     TITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRS
                     VSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTQH
                     TMYFA"
     exon            421..586
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            587..707
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            708..889
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            890..1026
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1161
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..1249
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            1250..1397
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            1398..1517
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            1518..1646
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            1647..1780
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            1781..1965
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            1966..2125
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            2126..2441
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            2442..2576
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            2577..2678
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            2679..2878
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            2879..7272
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7024..7029
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /note="hexamer: AATAAA"
     polyA_site      7049
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /note="major polyA site"
     regulatory      7251..7256
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /note="hexamer: AATAAA"
     polyA_site      7272
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /note="major polyA site"
ORIGIN      
        1 gttccccgac ccgcctcgcc gcctgccggg ctctgtggac cccgcgcccg gggccgcgca
       61 cgccccctcc gcccgccggg accctgggtc cgcgcacccc gcgccccctt ccctcccggc
      121 gggcgcgcgc gctggctccc gctcccgctc ccgttggcgg cggcggcggc ggcggcggcg
      181 gggattgttt ttgttgtcgc tgaggccgga agagccggag ccgggtccct gtccccgggc
      241 cgggcgccgc cgccgccccc tgcccagcgc ccgcgtctcc gcggcgccac cccagcgcca
      301 atattccgga gatcaagcgt tacgcggcgg cggcggcggc ggcggcgggg cccggagcgg
      361 gaggcgccgg ggaccggggc gaggcggccc ccgccgccgc catggaggcg ctgggacccg
      421 gacctccggc tagcctgttt cagccacctc gtcgtcctgg ccttggaact gttggaaaac
      481 caattcgact gttagccaat cattttcagg ttcagattcc taaaatagat gtgtatcact
      541 atgatgtgga tattaagcct gaaaaacggc ctcgtagagt caacagggag gtagtagata
      601 caatggtgcg gcacttcaag atgcaaatat ttggtgatcg gcagcctggg tatgatggca
      661 aaagaaacat gtacacagca catccactac caattggacg ggatagggtt gatatggagg
      721 tgactcttcc aggcgagggt aaagaccaaa catttaaagt gtctgttcag tgggtgtcag
      781 ttgtgagcct tcagttgctt ttagaagctt tggctgggca cttgaatgaa gtcccagatg
      841 actcagtaca agcacttgat gttatcacaa gacaccttcc ctccatgagg tacaccccag
      901 tgggccgttc ctttttctca cccccggaag gttactacca ccctctggga gggggcaggg
      961 aggtctggtt tggttttcat cagtctgtga gacctgccat gtggaatatg atgctcaaca
     1021 ttgatgtatc tgcaactgct ttctaccggg ctcagcctat cattgagttc atgtgtgagg
     1081 ttttagacat tcagaacatc aatgaacaga ccaaacctct aacagactcc cagcgtgtca
     1141 aatttaccaa agaaatcaga ggtctcaaag ttgaggtgac ccactgtgga cagatgaaac
     1201 gaaaataccg agtttgtaat gtgactagac ggccagccag tcatcaaact tttcctttgc
     1261 agctagaaaa cggtcaagct atggaatgta cagtagctca atattttaag caaaagtata
     1321 gtctgcaact gaaatacccc catcttccct gtctccaagt gggacaagaa caaaagcata
     1381 catacttgcc actcgaggtc tgtaatatag tggcaggaca gcgatgtatc aagaagctca
     1441 cagacaatca gacttccaca atgatcaaag ctacagcaag atctgctcct gacagacagg
     1501 aagagatcag tagactggtg aagagcaaca gtatggtggg tggacctgat ccatacctta
     1561 aagaatttgg tattgttgtc cacaatgaaa tgacagagct cacaggcagg gtacttccag
     1621 caccaatgct gcaatatgga ggccggaata aaacagtagc cacacccaac cagggtgtct
     1681 gggacatgcg aggaaagcag ttttatgctg gcattgaaat taaagtttgg gcagttgctt
     1741 gttttgcacc tcagaaacaa tgtagggaag atttactaaa gagtttcact gaccagctgc
     1801 gtaaaatctc taaggatgca ggaatgccca tccagggtca gccatgtttc tgcaagtatg
     1861 cacaaggtgc agacagtgtg gagcctatgt ttaaacatct gaaaatgact tatgtgggcc
     1921 tacagctaat agtggttatc ctgcctggaa agacaccagt atatgcggag gtgaaacgtg
     1981 ttggagatac ccttctaggt atggccacac agtgtgtcca ggtaaaaaat gtagtgaaga
     2041 cctcacctca aaccctttcc aatctttgcc tgaagataaa tgcaaaactt ggaggaatta
     2101 acaatgtgct tgtgcctcat caaaggccct cggtgttcca gcagcctgtc atcttcctgg
     2161 gagcggatgt cacacacccc ccagcagggg atgggaagaa accttccatt gctgctgtgg
     2221 ttggcagtat ggatggccac cccagccggt actgtgccac cgttcgggtg cagacttccc
     2281 ggcaggagat ctcccaagag ctcctctaca gtcaagaggt catccaggac ctgactaaca
     2341 tggttcgaga gctgctgatt cagttctaca aatccacacg cttcaaaccc actcggatca
     2401 tctattaccg tggaggggta tctgagggac aaatgaaaca ggtagcttgg ccagaactaa
     2461 tagcaattcg aaaggcatgt attagcttgg aagaagatta ccggccagga ataacttata
     2521 ttgtggtgca aaaaagacat cacacacgac tcttctgtgc agataaaaca gaaagggtag
     2581 ggaaaagtgg caatgtacca gcaggcacta cagtggatag taccatcaca catccatctg
     2641 agtttgactt ttacctctgt agtcatgcag gaattcaggg aaccagccgt ccctcacatt
     2701 accaggtctt gtgggatgac aactgcttca ctgcagatga actccagcta ctgacttacc
     2761 agctgtgtca cacctatgtg aggtgcactc gctcagtctc tattccagcc cctgcatatt
     2821 atgcccggct tgtagcattt agggcaaggt atcatctggt ggataaagat catgacagtg
     2881 cggaaggcag tcatgtgtca ggacagagca acggccggga tcctcaggcc ttggctaagg
     2941 ctgtgcaaat ccaccatgat acccagcaca cgatgtattt tgcctgagag tctcagaaaa
     3001 agaactcaac caatttggca ccccatgcag cctcaaaatg tttcaaatgc ctaccgcctc
     3061 tagatcgagc cacgttgact tcaggtggtc ttctaccagc agctcggaat agttgcactg
     3121 aatctatact ttgcagcact gtctgatgcg tcaacaaaat tgagccattt ttttaaagta
     3181 atagatacta atagattatc ttttctgatg cactggactg aatttttacc tcaatgtgca
     3241 aaggctgatc agcctgaact ttctaaagga ctttacaaga agggtttcta tggaatattt
     3301 tgctagctgt ggtgttcacc gcatccctct agtcttagaa gagaagatta ttcctttttt
     3361 ctgtagtcat tgagtggggt atatgcataa gtgggagaga aaaaccaaac aatctacttc
     3421 agttcagtgt aactatttaa ttgtgtgggt ccatagactt tttaatgaag atttctgact
     3481 ttgccactgt aaatgccttt aatgagcatt aatttttgaa agttttacag agttttatta
     3541 gttttactac tttatcttat tgatctctgc agacttgtgc tggtgcaagt acatgctgac
     3601 aggcaatttg cactatattt ggctgcagat tcaaataggc agttctctta tttggtcgac
     3661 ttttgtgaat gtgtgacata aacagatgta atgcatgtca cagtgacgga gataacactg
     3721 ccatgataaa agtactcatg tttcccctat ttggaaaaaa aaattgcttt tagtattttt
     3781 ccaagttttc aaaagtgccc attttatgct gctgtgtggt ttgttaaatc taaatgattt
     3841 ttaaactttt ctcatagaaa cttaactttg gcaataaaca ttttttaagg tctctcctct
     3901 tccttcccca acaatgtagt tttctagatg agatttcagt atgattttat cagctatttc
     3961 ttatatatca taatctggca atttctgaaa ccagtcagaa aagacatata tttccatgcc
     4021 tttttaaaga attattttta cctgttttat attcacagtt ggtgtcctgt cactttgttt
     4081 taaaataagc tagaaccagg atgcttcctt attggaaagg ctttgccagt ccttaaggta
     4141 caatgagtga tgctgcaact tgcaaatgta ccagcattta aaatttcttt tcctgggaat
     4201 caaggtaact cacacgacta cttgcagtac aaattttttg cttctctttc gacatgaaat
     4261 gtacttggtg ggttttcctt ccttttatta aactaaaact gtgtggaaaa ggttgatttt
     4321 tcttaataga ttctggattc cactcacagg gagatgggat tcctttcttg tccaaacaaa
     4381 gggagttgct ctaaagcgag catcagttaa aatgtagggg aaaatgtatt ctgtatgtag
     4441 tgtagataat actttgtgaa tggacaacag ttacatagtg tcttggtagc accagccagt
     4501 gtttcccagc ccagagttta ctgacttgta attctctatc caggacatca ggagtgaggg
     4561 atggaaaagg ggataaaagg cctggagcca cactctatat ggctgaggaa ggagctaggg
     4621 aaagagatag acacaatggg gtgaaaacaa ttttgccgcc cctgcctgga aactgctggt
     4681 ggggaaatga tggtgaaggg tgtttgcttg aactcatgag acggtccaaa tctcataaac
     4741 tagccttctc aaaatcccag caatataaag cactgttttt cttcagtcgt ttaaataaac
     4801 ttgtaaatac attgaagttt acatttccat gtgctgttga tcaacggcgc cacctgtgtt
     4861 tgctgagact ggtctctgag agagttttat gtcgatcgag gcctgttgct cattaagctg
     4921 agtgcagacg tccttaatcc ctgcagatag tgggatgaaa gcaagttctg taaaatgaag
     4981 cacaacatag ccaggagtga agagatggca gatgttaatt tcttaaaatt ttcctttttt
     5041 tttttttttt ttgcaaatac ctcacttgga taatacaaat caggacatgt tggtgagggg
     5101 ctggctgctt ggctgctgct tttattcaca cttgttcagg gagagcacag agaataccct
     5161 tcagcatggc cactctggac ccaagaggag gaaaaaacgg aagctttttt aggagaagga
     5221 ggacttgctg gaatccaaga tgtgaagaac tcttggtgac tggctggctg gccagcgggc
     5281 accacacctg cacttgagtg cctcgggctt gcttattaat aacgcacaga agaagcatca
     5341 cttctcacca tctcctggca tgtatggagc agggagcaag gctatgttcc cgaaagccct
     5401 gattagcttg tcttgctcta cgctcgacct cgaacaatac agcttgtaag acctcagtca
     5461 gtcctggtac actggggtaa atcagtgtgg tggtggaaga aaagccggga ggcctatttt
     5521 tataggaaaa tagtaccatt aaaggtgcaa tgctgtcatt ttggacaaaa ggaaagaaat
     5581 ttggaagagc tgggattatt ggcaaaaact tgctgagggg tgaatcttta gagctaatca
     5641 ctgcagaatc agagttgcag caatatgatt gcaagcaagt ctgaatgcat agttttactt
     5701 taattcggct tgacaatttg aataggtaac cccctcggga ctgactgaat cgttatgcat
     5761 gtgtcaatca atgggaggtg tgcattctct agaagtagtt ttaggctgtt tggtttggtt
     5821 ttggttttgt tttttaaatg cagcctgttg aggcactgtc atgtggtgtg aaagatttaa
     5881 atgtagcaac gctaaagagt aataatttgg ggataagaag tggggacatt ggccgggcgc
     5941 ggtggctcac gcctgtaatt ccagcacttt gggaggccta ggtgggcgga tcagctgagg
     6001 tcgagagttc gaggccagtc tgaccaacat ggtgaaaccc catctctact aaaaatacag
     6061 aaaattagct gggtctggtg gtggatgcct gtaatcccag ctactcggga ggctgaggca
     6121 ggagaattgc ttgaacccag gaggtggacg gtgcagtgag ctattactcc agcctgggca
     6181 acaagagcga aactctgtct caaaaaaaaa aagggggggg ggggacatta agagagtggt
     6241 gatgacccaa atgttgaacc aattatgttt tggtggtggt gttcttagct gttgaatcct
     6301 gaatggttta taaagtgaac tagctggctt aatgcagcca gcgttctggg cagcagaaca
     6361 tattcattct tactgtaaat tctatttgct gcttccaaag gtgatgattt tcaagcagac
     6421 atgttctata tggtctgtgt tttaggatct ggtgcccagc ctctatcaga gcttgcctac
     6481 ctggcaaagc tgcctaccct tcaagtggga aaatataatc cactgtttaa caaggctcac
     6541 cctctccacc ctgtcctaac gaccttttgt gaatgtgctg tgatattttc ttgctcaata
     6601 gcaaggtggt agctctgctt tcattttaag aaagtggagg ctgagggcat tgtatcaata
     6661 ctgttgcaac tccaagaagt tttccttgta aaattaaagg aaagatcttg ttattgatta
     6721 accattttct tatgccttgc tattgacata ttcatgctct ttctacgtct agtggctgaa
     6781 aatgtttgca tttgttcatt tgactaatgg tgtgattttt gtttctatat tattagacct
     6841 gtaatgtttt aaaatgtatt ttattaaatt tggactggat gtatgtctct agcaatacga
     6901 ggtactttct aaactattaa gggaggggtt gtatcctcat gttgagataa gatgatggtc
     6961 gtttaaattt tgcaattttt tttggcctgc agggatattt tgtgtttatg tgtccaaaaa
     7021 aggaataaat tggcattctt gtgccaaaag ttgtttttcc tgtcaattgt ctaataagta
     7081 tgcagtacac tgtaatggca acatacatgg ttgctttata aaaacagttt cctcagtatg
     7141 agaaatttta caaagaacag tggaaaaact ttgtgttttt aactcttggg tctccctatt
     7201 tttaaaaatt gctatttggt atacaattat tatgtgtcaa ttaaaactaa aataaaactt
     7261 ttaaaaaaga aa
//
